CN101226117B - Agent for preventing EDTA-K2 agent from crystallizing in vitro - Google Patents
Agent for preventing EDTA-K2 agent from crystallizing in vitro Download PDFInfo
- Publication number
- CN101226117B CN101226117B CN2007100507073A CN200710050707A CN101226117B CN 101226117 B CN101226117 B CN 101226117B CN 2007100507073 A CN2007100507073 A CN 2007100507073A CN 200710050707 A CN200710050707 A CN 200710050707A CN 101226117 B CN101226117 B CN 101226117B
- Authority
- CN
- China
- Prior art keywords
- edta
- agent
- copolyvidone
- reagent
- blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides an agent which can effectively prevent EDTA-K2 agent from crystallizing in glass and plastic tubes, wherein the agent is composed of EDTA-K2 and copolymer povidone which are rationed at 8-12:1-3, while the best embodiment is 7:2. The invention adds copolymer povidone into EDTA-K2 to keep the anticoagulation of diamine tetracetate and prevent single EDTA-K2 agent from crystallizing later, to reduce dissolution speed and avoid affecting blood anticoagulation, while EDTA-K2 crystal can generate some heat in dissolution to cause hemolysis to affect check result.
Description
Technical field
The present invention provides a kind of reagent that can effectively prevent EDTA-K2 reagent at glass and plastic test tube intercrystalline.
Background technology
EDTA-K2 is an edetate.Ethylenediamine tetraacetic acid has another name called edetic acid, Wei Er diluted acid, EDTA and EDTA acid etc., and ethylenediamine tetraacetic acid and salt thereof are that the important blood that becomes more meticulous is pieced together and sequestrant the English name Ethylenediaminetetraacetic of ethylenediamine tetraacetic acid, molecular formula: C
10H
16N
2O
8Molecular weight M=292.2, technical indicator GB the T1401-1998. ethylenediamine tetraacetic acid be a kind of white crystalline powder, 240 ℃ of fusing points (decomposition), solubleness is 0.5g/L (25 ℃) in the water, is insoluble to common organic solvents, can be dissolved in 160 parts of boiling water, be dissolved in alkaline solutions such as NaOH, just show the tendency of taking off carboxyl at 150 ℃.Blood routine checks that used anti-coagulants is generally EDTA-K2, recommends to use for international blood standard committee (ICSH), and this anti-coagulants does not influence white blood cell count(WBC) and size, and is also minimum to erythrocytic form influence, and can suppress hematoblastic gathering.At present, be added on the interior single EDTA-K2 reagent of glass and plastics and can separate out crystal gradually over time, dissolution velocity is slowed down, influence the blood anticoagulant effect.And the EDTA-K2 crystal can produce certain heat in course of dissolution, cause haemolysis, influences testing result.
Summary of the invention
In order to solve the problem of existing EDTA-K2 crystallization, now add a kind of reagent--copolyvidone is mixed use with EDTA-K2, thoroughly solve the problem of EDTA-K2 crystallization, guarantees blood clinical detection result's accuracy.
Technical scheme of the present invention realizes by following content:
A kind of reagent that prevents EDTA-K2 reagent at glass and plastic test tube intercrystalline is made of EDTA-K2 and copolyvidone, and wherein the ratio of EDTA-K2 and copolyvidone is 8～12: 1～3.
Preferably, the ratio of EDTA-K2 of the present invention and copolyvidone is 6～8: 2～3.
Most preferably, the ratio of EDTA-K2 of the present invention and copolyvidone is 7: 2.
EDTA-K2 is an edetate, and ethylenediamine tetraacetic acid and salt thereof are that the important blood that becomes more meticulous is pieced together and sequestrant, check used anti-coagulants for blood routine, can suppress hematoblastic gathering.
Copolyvidone has excellent biological compatibility, and it can prevent that EDTA-K2 from separating out crystal.And prevent in the blood dissolves process heat that can produce.
The present invention adds copolyvidone in EDTA-K2, the anticoagulation that had both kept ethylenediaminotetraacetate (edetate), prevented that again single EDTA-K2 reagent from can separate out crystal over time gradually, dissolution velocity is slowed down, influence the blood anticoagulant effect, and the EDTA-K2 crystal can produce certain heat in course of dissolution, cause haemolysis, influences the problem of testing result.
Embodiment
Embodiment 1
A kind of reagent that prevents EDTA-K2 reagent at glass and plastic test tube intercrystalline is made of EDTA-K2 and copolyvidone, and wherein the ratio of EDTA-K2 and copolyvidone is 8: 3.EDTA-K2 is an edetate, and ethylenediamine tetraacetic acid and salt thereof are that the important blood that becomes more meticulous is pieced together and sequestrant, check used anti-coagulants for blood routine, can suppress hematoblastic gathering.
Copolyvidone has excellent biological compatibility, and it can prevent that EDTA-K2 from separating out crystal.And prevent in the blood dissolves process heat that can produce.
The present invention adds copolyvidone in EDTA-K2, the anticoagulation that had both kept ethylenediaminotetraacetate (edetate), prevented that again single EDTA-K2 reagent from can separate out crystal over time gradually, dissolution velocity is slowed down, influence the blood anticoagulant effect, and the EDTA-K2 crystal can produce certain heat in course of dissolution, cause haemolysis, influences the problem of testing result.
Embodiment 2
A kind of reagent that prevents EDTA-K2 reagent at glass and plastic test tube intercrystalline is made of EDTA-K2 and copolyvidone, and wherein the ratio of EDTA-K2 and copolyvidone is 12: 1.EDTA-K2 is an edetate, and ethylenediamine tetraacetic acid and salt thereof are that the important blood that becomes more meticulous is pieced together and sequestrant, check used anti-coagulants for blood routine, can suppress hematoblastic gathering.Copolyvidone has excellent biological compatibility, and it can prevent that EDTA-K2 from separating out crystal.And prevent in the blood dissolves process heat that can produce.The present invention adds copolyvidone in EDTA-K2, the anticoagulation that had both kept ethylenediaminotetraacetate (edetate), prevented that again single EDTA-K2 reagent from can separate out crystal over time gradually, dissolution velocity is slowed down, influence the blood anticoagulant effect, and the EDTA-K2 crystal can produce certain heat in course of dissolution, cause haemolysis, influences the problem of testing result.
Embodiment 3
A kind of reagent that prevents EDTA-K2 reagent at glass and plastic test tube intercrystalline is made of EDTA-K2 and copolyvidone, and wherein the ratio of EDTA-K2 and copolyvidone is 6: 3.EDTA-K2 is an edetate, and ethylenediamine tetraacetic acid and salt thereof are that the important blood that becomes more meticulous is pieced together and sequestrant, check used anti-coagulants for blood routine, can suppress hematoblastic gathering.Copolyvidone has excellent biological compatibility, and it can prevent that EDTA-K2 from separating out crystal.And prevent in the blood dissolves process heat that can produce.The present invention adds copolyvidone in EDTA-K2, the anticoagulation that had both kept ethylenediaminotetraacetate (edetate), prevented that again single EDTA-K2 reagent from can separate out crystal over time gradually, dissolution velocity is slowed down, influence the blood anticoagulant effect, and the EDTA-K2 crystal can produce certain heat in course of dissolution, cause haemolysis, influences the problem of testing result.
Embodiment 3
A kind of reagent that prevents EDTA-K2 reagent at glass and plastic test tube intercrystalline is made of EDTA-K2 and copolyvidone, and wherein the ratio of EDTA-K2 and copolyvidone is 8: 2.EDTA-K2 is an edetate, and ethylenediamine tetraacetic acid and salt thereof are that the important blood that becomes more meticulous is pieced together and sequestrant, check used anti-coagulants for blood routine, can suppress hematoblastic gathering.Copolyvidone has excellent biological compatibility, and it can prevent that EDTA-K2 from separating out crystal.And prevent in the blood dissolves process heat that can produce.The present invention adds copolyvidone in EDTA-K2, the anticoagulation that had both kept ethylenediaminotetraacetate (edetate), prevented that again single EDTA-K2 reagent from can separate out crystal over time gradually, dissolution velocity is slowed down, influence the blood anticoagulant effect, and the EDTA-K2 crystal can produce certain heat in course of dissolution, cause haemolysis, influences the problem of testing result.
Embodiment 3
A kind of reagent that prevents EDTA-K2 reagent at glass and plastic test tube intercrystalline is made of EDTA-K2 and copolyvidone, and wherein the ratio of EDTA-K2 and copolyvidone is 7: 2.EDTA-K2 is an edetate, and ethylenediamine tetraacetic acid and salt thereof are that the important blood that becomes more meticulous is pieced together and sequestrant, check used anti-coagulants for blood routine, can suppress hematoblastic gathering.Copolyvidone has excellent biological compatibility, and it can prevent that EDTA-K2 from separating out crystal.And prevent in the blood dissolves process heat that can produce.The present invention adds copolyvidone in EDTA-K2, the anticoagulation that had both kept ethylenediaminotetraacetate (edetate), prevented that again single EDTA-K2 reagent from can separate out crystal over time gradually, dissolution velocity is slowed down, influence the blood anticoagulant effect, and the EDTA-K2 crystal can produce certain heat in course of dissolution, cause haemolysis, influences the problem of testing result.
Claims (2)
1. reagent that prevents the crystallization in vitro of EDTA-K2 reagent, it is characterized in that: described reagent is made of EDTA-K2 and copolyvidone, and wherein the ratio of EDTA-K2 and copolyvidone is 8～12: 1～3.
2. the reagent that prevents the crystallization in vitro of EDTA-K2 reagent according to claim 1 is characterized in that: the best proportion of described EDTA-K2 and copolyvidone is 7: 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100507073A CN101226117B (en) | 2007-12-05 | 2007-12-05 | Agent for preventing EDTA-K2 agent from crystallizing in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100507073A CN101226117B (en) | 2007-12-05 | 2007-12-05 | Agent for preventing EDTA-K2 agent from crystallizing in vitro |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101226117A CN101226117A (en) | 2008-07-23 |
| CN101226117B true CN101226117B (en) | 2010-08-11 |
Family
ID=39858237
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100507073A Active CN101226117B (en) | 2007-12-05 | 2007-12-05 | Agent for preventing EDTA-K2 agent from crystallizing in vitro |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101226117B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101559244B (en) * | 2009-05-21 | 2012-07-11 | 安徽信灵检验医学科技有限公司 | Ethylene diamine tetraacetic acid sylvite metacrystalline excipient |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1222677A (en) * | 1997-10-31 | 1999-07-14 | 中国人民武装警察部队江苏省总队医院 | Diluent and blood solvent cleaning liquid for blood cell counter and their preparation |
| CN1238455A (en) * | 1998-06-04 | 1999-12-15 | 梁建华 | Diluent and cleaning liquid for blood analysis counter |
-
2007
- 2007-12-05 CN CN2007100507073A patent/CN101226117B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1222677A (en) * | 1997-10-31 | 1999-07-14 | 中国人民武装警察部队江苏省总队医院 | Diluent and blood solvent cleaning liquid for blood cell counter and their preparation |
| CN1238455A (en) * | 1998-06-04 | 1999-12-15 | 梁建华 | Diluent and cleaning liquid for blood analysis counter |
Non-Patent Citations (2)
| Title |
|---|
| JP特开2007-302560A 2007.11.22 |
| 周卫东.关注聚维酮应用细节.中国医药报.2006,A8. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101226117A (en) | 2008-07-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Benedict | The detection and estimation of glucose in urine | |
| CN101226117B (en) | Agent for preventing EDTA-K2 agent from crystallizing in vitro | |
| CN105062024B (en) | High transparency high-temperature resistant lactic acid composite material and preparation method thereof | |
| CN103063661A (en) | Tetramethylbenzidine (TMB) coloration solution and preparation method thereof | |
| CN101975850A (en) | Diluent for blood cell analyzer | |
| Weichselbaum et al. | Determination of hippuric acid in urine | |
| WO2023201840A1 (en) | Universal sample diluent for immunochromatography detection and preparation method therefor | |
| RU2551570C2 (en) | Solution for fixing biological cells | |
| Harkness et al. | The equilibrium of para and ortho hydrogen | |
| WO2023246254A1 (en) | Detection kit of sperm sialidase 1/3 and preparation method therefor, and method for detecting expression level of sperm sialidase 1/3 | |
| CN101029872A (en) | Liquid for testing hydrogen sulfide and its colorimetric testing tube | |
| CN102645530B (en) | Preparation method of enzyme conjugate diluent in thyroxine enzyme-linked immune body in-vitro diagnostic reagent kit | |
| Gaitskell et al. | Drinking and urine production in the European eel (Anguilla anguilla L.) | |
| CN109401691A (en) | High bond strength composite board binder and preparation method thereof | |
| CN101838676B (en) | Quick detection method of coliforms in seasonings | |
| WO2018040820A1 (en) | Process for measuring degree of deacetylation of chitosan oligosaccharide using acid-base indicator method | |
| CN104459110A (en) | Sing-component TMB color development solution | |
| JP5335288B2 (en) | Glass electrode and its response glass | |
| CN206714762U (en) | Add the vacuum blood collection tube of anti-EDTA dependence syndromes reagent | |
| CN103728409A (en) | Method for measuring content of calcium ions in solution | |
| CN114403130B (en) | Liquid-based cell preservation solution and preparation method and application thereof | |
| CN103018432A (en) | Sheath fluid for hematology analyzer and preparation method of sheath fluid | |
| TH14498A3 (en) | Magnesium Field Test Kit in Latex | |
| CN104032407B (en) | A kind of preparation method of high oil absorption fibrous material | |
| CN203324170U (en) | Analysis instrument capable of fast measuring available chlorine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: CHENGDU RICH SCIENCE INDUSTRY CO., LTD. Free format text: FORMER NAME: CHENGDU RUIQI SCIENCE AND TECHNOLOGY INDUSTRIAL CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: 611731, 66, Tian Qin East Street, Chengdu hi tech Zone (West District, Sichuan) Patentee after: CHENGDU RICH SCIENCE INDUSTRY CO., LTD. Address before: 611731, No. 21, West core road, hi tech West District, Sichuan, Chengdu Patentee before: Chengdu Ruiqi Science and Technology Industrial Co., Ltd. |
|
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 610000, 66, Tian Qin East Street, Chengdu hi tech Zone (West District, Sichuan) Patentee after: Chengdu Ruiqi Medical Technology Co., Ltd Address before: 611731, 66, Tian Qin East Street, Chengdu hi tech Zone (West District, Sichuan) Patentee before: CHENGDU RICH SCIENCE INDUSTRY CO., LTD. |