CN101975850A - Diluent for blood cell analyzer - Google Patents
Diluent for blood cell analyzer Download PDFInfo
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- CN101975850A CN101975850A CN2010102786909A CN201010278690A CN101975850A CN 101975850 A CN101975850 A CN 101975850A CN 2010102786909 A CN2010102786909 A CN 2010102786909A CN 201010278690 A CN201010278690 A CN 201010278690A CN 101975850 A CN101975850 A CN 101975850A
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Abstract
The invention provides a diluent for a blood cell analyzer. The diluent consists of edentate, sodium sulfate, sodium chloride, dimethylol urea, biological buffer, a betain type nonionic surfactant, sodium azide and water. The diluent solves the problems that the osmotic pressure of a bottom layer is higher than that of an upper layer and the solution is not uniform or stable when the diluent for the blood cell analyzer stands for a long time.
Description
Technical field
The invention belongs to the blood cell analysis field, particularly relate to the dilution that a kind of cellanalyzer is used.
Background technology
Dilution is the most frequently used a kind of reagent of cellanalyzer in the clinical examination, is the key reagents of finishing blood cell differential or counting.Before carrying out blood cell analysis, cleaning fluid and dilution cooperatively interact the container of instrument detecting unit and pipeline are cleaned up, and with this background value that comes lowering apparatus, avoid the interference of last detection sample to this detection.In addition, dilution also plays keeps leucocyte, the original form of blood platelet, guarantees the effect that instrument is accurately classified to leucocyte.
At present there is following shortcoming in the dilution that uses: place component in the dilution for a long time easily because of the action of gravity sedimentation, thereby the osmotic pressure that causes the dilution bottom is apparently higher than the upper strata, the solution heterogeneity is stable, and then cause that leucocyte, HRD of RBC showed move to left, phenomenons such as the false rising of lymphocyte percentage.
Summary of the invention
Long-time placement the purpose of this invention is to provide a kind of cellanalyzer dilution, can overcome above shortcoming, even also can keep the solution stable homogeneous.
To achieve these goals, the technical scheme that the present invention proposes is: the cellanalyzer dilution comprises following composition: edetate, sodium sulphate, sodium chloride, dihydroxy methyl urea, biological buffer, betaine type non-ionic surfactant, Sodium azide, water.Described biological buffer is N-(2-acetamide) iminodiacetic acid or hydroxyethyl piperazine second thiosulfonic acid.Described betaine type non-ionic surfactant is dodecyl sulfobetaines or cocounut oil acyl propyl group sulfobetaines.Cellanalyzer with the content of each composition of dilution is: (1) edetate, content are 0.1～1g/L; (2) sodium sulphate, content are 4～10g/L; (3) sodium chloride, content are 1～6g/L; (4) dihydroxy methyl urea, content are 1～5g/L; (5) biological buffer, content are 5～10g/L; (6) betaine type non-ionic surfactant, content are 0.1～1g/L; (7) Sodium azide, content are 0.1～1g/L; All the other are water.
In the technical scheme of the present invention, edetate has anticoagulant effect, can prevent that blood from solidifying in testing process.The osmotic pressure that sodium sulphate, sodium chloride and dihydroxy methyl urea are used to control dilution is in 290～350mOsm (milliosmol), and haemocyte can be kept normal volume and form in this osmotic pressure scope.The pH value that biological buffer is used to control dilution is 6.8～7.4, to keep the normal physiological state of haemocyte.It is constant that the betaine type non-ionic surfactant is used to keep dilution osmotic pressure, makes the dilution stable homogeneous, avoids component because long-time the placement interacts and sedimentation.Sodium azide has the corrosion-resistant effect.Biological buffer is N-(2-acetamide) iminodiacetic acid or hydroxyethyl piperazine second thiosulfonic acid, the betaine type non-ionic surfactant is dodecyl sulfobetaines or cocounut oil acyl propyl group sulfobetaines, cellanalyzer better effects if when the content of each composition of dilution is in aforementioned range.
From the above advantage of the present invention as can be known is to overcome the prior art shortcoming, even make the long-time placement of dilution also can keep the solution stable homogeneous.
Embodiment
The cellanalyzer dilution of embodiment comprises following composition: (1) edetate, content are 0.5g/L; (2) sodium sulphate, content are 6g/L; (3) sodium chloride, content are 4g/L; (4) dihydroxy methyl urea, content are 2g/L; (5) biological buffer is a hydroxyethyl piperazine second thiosulfonic acid, and content is 6g/L; (6) the betaine type non-ionic surfactant is the dodecyl sulfobetaines, and content is 0.5g/L; (7) Sodium azide, content is: 0.5g/L; All the other are water.
Cellanalyzer of the present invention is placed one-year age with dilution, and the dilution bottom is identical with top layer osmotic pressure, and solution keeps the stable homogeneous state.
Claims (5)
1. the cellanalyzer dilution is characterized in that comprising following composition: edetate, sodium sulphate, sodium chloride, dihydroxy methyl urea, biological buffer, betaine type non-ionic surfactant, Sodium azide, water.
2. according to the described cellanalyzer dilution of claim 1, it is characterized in that described biological buffer is N-(2-acetamide) iminodiacetic acid or hydroxyethyl piperazine second thiosulfonic acid.
3. according to claim 1 or 2 described cellanalyzer dilutions, it is characterized in that described betaine type non-ionic surfactant is dodecyl sulfobetaines or cocounut oil acyl propyl group sulfobetaines.
4. according to claim 1 or 2 described cellanalyzer dilutions, it is characterized in that cellanalyzer with the content of each composition of dilution is: (1) edetate, content are 0.1～1g/L; (2) sodium sulphate, content are 4～10g/L; (3) sodium chloride, content are 1～6g/L; (4) dihydroxy methyl urea, content are 1～5g/L; (5) biological buffer, content are 5～10g/L; (6) betaine type non-ionic surfactant, content are 0.1～1g/L; (7) Sodium azide, content are 0.1～1g/L; All the other are water.
5. according to the described cellanalyzer dilution of claim 3, it is characterized in that cellanalyzer with the content of each composition of dilution is: (1) edetate, content are 0.1～1g/L; (2) sodium sulphate, content are 4～10g/L; (3) sodium chloride, content are 1～6g/L; (4) dihydroxy methyl urea, content are 1～5g/L; (5) biological buffer, content are 5～10g/L; (6) betaine type non-ionic surfactant, content are 0.1～1g/L; (7) Sodium azide, content are 0.1～1g/L; All the other are water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102786909A CN101975850A (en) | 2010-09-13 | 2010-09-13 | Diluent for blood cell analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102786909A CN101975850A (en) | 2010-09-13 | 2010-09-13 | Diluent for blood cell analyzer |
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| CN101975850A true CN101975850A (en) | 2011-02-16 |
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| CN2010102786909A Pending CN101975850A (en) | 2010-09-13 | 2010-09-13 | Diluent for blood cell analyzer |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103502813A (en) * | 2011-05-09 | 2014-01-08 | 积水医疗株式会社 | Method for immunologically measuring soluble LR11 |
| CN103743616A (en) * | 2013-12-19 | 2014-04-23 | 深圳市雷诺华科技实业有限公司 | Diluent for blood analyzer |
| WO2014187013A1 (en) * | 2013-05-23 | 2014-11-27 | 北京博晖创新光电技术股份有限公司 | Reagent for processing trace whole blood and application thereof |
| CN107014664A (en) * | 2017-03-22 | 2017-08-04 | 安徽民泰医药科技有限公司 | Blood cell analysis dilution |
| CN107991151A (en) * | 2017-11-22 | 2018-05-04 | 中山市创艺生化工程有限公司 | A kind of reagent for hemocyte analyzers |
| CN110018299A (en) * | 2013-03-12 | 2019-07-16 | 雅培实验室 | For analyzing reagent, system and the method for red blood cell and blood platelet |
| CN111413176A (en) * | 2020-05-09 | 2020-07-14 | 河北艾驰生物科技有限公司 | Quality control substance for red blood cells and white blood cells for analyzing urine visible components and preparation method thereof |
| CN111521789A (en) * | 2020-04-20 | 2020-08-11 | 山东第一医科大学(山东省医学科学院) | Immunofluorescence kit and detection method for detecting expression of peripheral blood circulating tumor cells CA199 of pancreatic cancer patient |
| CN112903408A (en) * | 2021-01-26 | 2021-06-04 | 金华市鑫科医疗器械有限公司 | Diluent for blood cell analyzer |
| CN112985966A (en) * | 2021-02-08 | 2021-06-18 | 桂林优利特医疗电子有限公司 | Diluent for analyzing urine visible components and preparation method thereof |
| WO2022001825A1 (en) * | 2020-07-01 | 2022-01-06 | 天津市肿瘤医院（天津医科大学肿瘤医院） | Kit for detecting e-cadherin expression of peripheral blood circulating tumor cells of pancreatic cancer patient and detection method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1238455A (en) * | 1998-06-04 | 1999-12-15 | 梁建华 | Diluent and cleaning liquid for blood analysis counter |
| CN1548942A (en) * | 2003-05-09 | 2004-11-24 | 上海捷瑞医用试剂有限公司 | Diluent for blood cell analysis instrument |
| CN1834612A (en) * | 2006-04-29 | 2006-09-20 | 北京索通医疗技术有限公司 | Multifunction dilutent for blood cell analyzer and prepn. method |
| CN101451120A (en) * | 2008-12-31 | 2009-06-10 | 厦门大学 | Abalone blood cell anticoagulation protecting agent and preparation method thereof |
-
2010
- 2010-09-13 CN CN2010102786909A patent/CN101975850A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1238455A (en) * | 1998-06-04 | 1999-12-15 | 梁建华 | Diluent and cleaning liquid for blood analysis counter |
| CN1548942A (en) * | 2003-05-09 | 2004-11-24 | 上海捷瑞医用试剂有限公司 | Diluent for blood cell analysis instrument |
| CN1834612A (en) * | 2006-04-29 | 2006-09-20 | 北京索通医疗技术有限公司 | Multifunction dilutent for blood cell analyzer and prepn. method |
| CN101451120A (en) * | 2008-12-31 | 2009-06-10 | 厦门大学 | Abalone blood cell anticoagulation protecting agent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 刘军等: "十二烷基磺基甜菜碱与其它类型表面活性剂的相互作用", 《山东大学学报（理学版）》 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103502813B (en) * | 2011-05-09 | 2016-10-12 | 积水医疗株式会社 | The immunological assay method of solubility LR11 |
| US9541550B2 (en) | 2011-05-09 | 2017-01-10 | Sekisui Medical Co., Ltd. | Method for immunologically measuring soluble LR11 |
| CN103502813A (en) * | 2011-05-09 | 2014-01-08 | 积水医疗株式会社 | Method for immunologically measuring soluble LR11 |
| US11378578B2 (en) | 2013-03-12 | 2022-07-05 | Abbott Laboratories | Reagents, systems and methods for analyzing erythrocytes and platelets |
| CN110018299A (en) * | 2013-03-12 | 2019-07-16 | 雅培实验室 | For analyzing reagent, system and the method for red blood cell and blood platelet |
| CN110018299B (en) * | 2013-03-12 | 2020-09-15 | 雅培实验室 | Reagent, system and method for analyzing red blood cells and platelets |
| WO2014187013A1 (en) * | 2013-05-23 | 2014-11-27 | 北京博晖创新光电技术股份有限公司 | Reagent for processing trace whole blood and application thereof |
| CN103743616A (en) * | 2013-12-19 | 2014-04-23 | 深圳市雷诺华科技实业有限公司 | Diluent for blood analyzer |
| CN107014664A (en) * | 2017-03-22 | 2017-08-04 | 安徽民泰医药科技有限公司 | Blood cell analysis dilution |
| CN107991151A (en) * | 2017-11-22 | 2018-05-04 | 中山市创艺生化工程有限公司 | A kind of reagent for hemocyte analyzers |
| CN111521789A (en) * | 2020-04-20 | 2020-08-11 | 山东第一医科大学(山东省医学科学院) | Immunofluorescence kit and detection method for detecting expression of peripheral blood circulating tumor cells CA199 of pancreatic cancer patient |
| CN111413176A (en) * | 2020-05-09 | 2020-07-14 | 河北艾驰生物科技有限公司 | Quality control substance for red blood cells and white blood cells for analyzing urine visible components and preparation method thereof |
| CN111413176B (en) * | 2020-05-09 | 2023-05-16 | 河北艾驰生物科技有限公司 | Erythrocyte and leucocyte quality control substance for urine component analysis and preparation method thereof |
| WO2022001825A1 (en) * | 2020-07-01 | 2022-01-06 | 天津市肿瘤医院（天津医科大学肿瘤医院） | Kit for detecting e-cadherin expression of peripheral blood circulating tumor cells of pancreatic cancer patient and detection method |
| CN112903408A (en) * | 2021-01-26 | 2021-06-04 | 金华市鑫科医疗器械有限公司 | Diluent for blood cell analyzer |
| CN112985966A (en) * | 2021-02-08 | 2021-06-18 | 桂林优利特医疗电子有限公司 | Diluent for analyzing urine visible components and preparation method thereof |
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Application publication date: 20110216 |