CN101225396A - 利用基因工程生产限制性内切酶的方法 - Google Patents
利用基因工程生产限制性内切酶的方法 Download PDFInfo
- Publication number
- CN101225396A CN101225396A CNA2008100576595A CN200810057659A CN101225396A CN 101225396 A CN101225396 A CN 101225396A CN A2008100576595 A CNA2008100576595 A CN A2008100576595A CN 200810057659 A CN200810057659 A CN 200810057659A CN 101225396 A CN101225396 A CN 101225396A
- Authority
- CN
- China
- Prior art keywords
- enzyme
- gene
- restriction
- carrier
- described method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2008100576595A CN101225396B (zh) | 2008-02-04 | 2008-02-04 | 利用基因工程生产限制性内切酶的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2008100576595A CN101225396B (zh) | 2008-02-04 | 2008-02-04 | 利用基因工程生产限制性内切酶的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101225396A true CN101225396A (zh) | 2008-07-23 |
CN101225396B CN101225396B (zh) | 2011-11-30 |
Family
ID=39857601
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2008100576595A Active CN101225396B (zh) | 2008-02-04 | 2008-02-04 | 利用基因工程生产限制性内切酶的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101225396B (zh) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106480076A (zh) * | 2016-11-25 | 2017-03-08 | 淮海工学院 | 一种利用甲基化酶的保护高效重组表达限制性内切酶的方法 |
CN106755002A (zh) * | 2016-11-25 | 2017-05-31 | 江苏愚公生命科技有限公司 | 利用甲基化酶的保护高效重组表达限制性内切酶的方法 |
CN106967743A (zh) * | 2017-05-02 | 2017-07-21 | 通用生物系统(安徽)有限公司 | 一种重组NotI限制性内切酶在大肠杆菌中的生产方法 |
CN107267545A (zh) * | 2017-07-26 | 2017-10-20 | 通用生物系统(安徽)有限公司 | 重组Bsa I限制性内切酶的制备方法 |
CN107267544A (zh) * | 2017-07-26 | 2017-10-20 | 通用生物系统(安徽)有限公司 | 重组EcoRV限制性内切酶的制备方法 |
CN107557372A (zh) * | 2017-08-30 | 2018-01-09 | 江苏愚公生命科技有限公司 | 用于表达限制性内切酶SacI的甲基化保护菌株的筛选方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3751923T2 (de) * | 1986-06-06 | 1997-04-03 | New England Biolabs Inc | Verfahren zur Klonierung von Restriktions-Modifikationssystemen |
-
2008
- 2008-02-04 CN CN2008100576595A patent/CN101225396B/zh active Active
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106480076A (zh) * | 2016-11-25 | 2017-03-08 | 淮海工学院 | 一种利用甲基化酶的保护高效重组表达限制性内切酶的方法 |
CN106755002A (zh) * | 2016-11-25 | 2017-05-31 | 江苏愚公生命科技有限公司 | 利用甲基化酶的保护高效重组表达限制性内切酶的方法 |
CN106480076B (zh) * | 2016-11-25 | 2020-01-24 | 莫纳(连云港)生物科技有限公司 | 一种利用甲基化酶的保护高效重组表达限制性内切酶的方法 |
CN106967743A (zh) * | 2017-05-02 | 2017-07-21 | 通用生物系统(安徽)有限公司 | 一种重组NotI限制性内切酶在大肠杆菌中的生产方法 |
CN107267545A (zh) * | 2017-07-26 | 2017-10-20 | 通用生物系统(安徽)有限公司 | 重组Bsa I限制性内切酶的制备方法 |
CN107267544A (zh) * | 2017-07-26 | 2017-10-20 | 通用生物系统(安徽)有限公司 | 重组EcoRV限制性内切酶的制备方法 |
CN107557372A (zh) * | 2017-08-30 | 2018-01-09 | 江苏愚公生命科技有限公司 | 用于表达限制性内切酶SacI的甲基化保护菌株的筛选方法 |
Also Published As
Publication number | Publication date |
---|---|
CN101225396B (zh) | 2011-11-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101225396B (zh) | 利用基因工程生产限制性内切酶的方法 | |
US11130955B2 (en) | Applications of CRISPRi in high throughput metabolic engineering | |
US11098305B2 (en) | Modular universal plasmid design strategy for the assembly and editing of multiple DNA constructs for multiple hosts | |
Williams | Restriction endonuclease: Classification, properties, and applications | |
Bair et al. | A type IV modification dependent restriction nuclease that targets glucosylated hydroxymethyl cytosine modified DNAs | |
US10196645B2 (en) | Expression vector | |
CN106480076A (zh) | 一种利用甲基化酶的保护高效重组表达限制性内切酶的方法 | |
WO2020206202A1 (en) | Methods for integrating a donor dna sequence into the genome of bacillus using linear recombinant dna constructs and compositions thereof | |
US20230220424A1 (en) | Rapid removal of a self-replicating fungal plasmid for efficient marker cycling | |
KR100244066B1 (ko) | D-N-카르바모일-α-아미노산의 제조법 | |
US5759839A (en) | Cloned SstI/SacI restriction-modification system | |
JP3953578B2 (ja) | 耐熱性ジアホラーゼ遺伝子 | |
CN118139979A (zh) | 具有hepn结构域的酶 | |
Liu et al. | A universal mini-vector and an annealing of PCR products (APP)-based cloning strategy for convenient molecular biological manipulations | |
EP1629100B1 (en) | Archaeon expression system | |
JPH0698779A (ja) | NaeI制限エンドヌクレアーゼ及びメチラーゼをクローン化し産生する方法 | |
CN118256532A (zh) | 一种在环状载体上引入目的基因的构建方法 | |
CN110643560A (zh) | 用于重组表达限制性内切酶的工程菌株及其构建方法 | |
US5516678A (en) | Method for producing the SSPI restriction endonuclease and methylase | |
WO2023046163A1 (zh) | 核酸文库构建和测序的方法和试剂盒 | |
US20230183662A1 (en) | Novel dna methyltransferase | |
US5731185A (en) | Isolated DNA encoding the hphi restriction endonuclease and related methods for producing the same | |
Xu et al. | Type II restriction of 2-aminoadenine (dZ) modified DNA and production of dZ-modified plasmid in E. coli | |
US20190048325A1 (en) | Bacteriophage engineering via semi-synthesis | |
Kumar et al. | Restriction Site Finder: A Tool for Finding Restriction Sites in Genome Using Perl |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: GPROAN BIOTECHNOLOGIES (SUZHOU) CO., LTD. Free format text: FORMER OWNER: GPROAN PROTEIN ENGINEERING (BEIJING) INC. Effective date: 20121015 |
|
C41 | Transfer of patent application or patent right or utility model | ||
COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 100085 HAIDIAN, BEIJING TO: 215425 SUZHOU, JIANGSU PROVINCE |
|
TR01 | Transfer of patent right |
Effective date of registration: 20121015 Address after: 215425 bio pharmaceutical industry park, Shaxi Town, Suzhou, Jiangsu, Taicang Patentee after: Kim Puno Biotechnology (Suzhou) Co., Ltd. Address before: 100085, D building 807L, 2 information road, Beijing, Haidian District Patentee before: Gproan Protein Engineering (Beijing) Inc. |
|
CP01 | Change in the name or title of a patent holder | ||
CP01 | Change in the name or title of a patent holder |
Address after: 215425 biological medicine industrial park, Shaxi Town, Taicang City, Suzhou City, Jiangsu Province Patentee after: Jiangsu jinpunuoan Biotechnology Co.,Ltd. Address before: 215425 biological medicine industrial park, Shaxi Town, Taicang City, Suzhou City, Jiangsu Province Patentee before: GPROAN BIOTECHNOLOGIES (SUZHOU), Inc. |