CN101223946A - Stabilization of enzyme treated bee milk - Google Patents
Stabilization of enzyme treated bee milk Download PDFInfo
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- CN101223946A CN101223946A CNA2008100034712A CN200810003471A CN101223946A CN 101223946 A CN101223946 A CN 101223946A CN A2008100034712 A CNA2008100034712 A CN A2008100034712A CN 200810003471 A CN200810003471 A CN 200810003471A CN 101223946 A CN101223946 A CN 101223946A
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Abstract
The invention provides an enzyme-treated royaljelly, which can particularly prevent deteriorating along with brown changing. Before, while or after the royaljelly is treated by peptidase, at least one kind group selected from the groups constituted by galactomannan, polyose containing galactomanna and xanthan gum is added, if necessary freeze drying is further performed, thus the stable enzyme-treated royaljelly composition for preventing coloring is produced.
Description
Technical field
The present invention relates to a kind of painted repressed enzyme treated bee milk composition and manufacture method thereof, in addition, the present invention relates to a kind of suppress enzyme treated bee milk through the time method of colouring that produces.
Background technology
Fresh royal jelly (giving birth to ロ one ヤ Le ゼ リ one) is useful natural material, and but then, known its causes allergic reaction sometimes.
Royal jelly as hypoallergenic formerization, the royal jelly of known a kind of so-called hypoallergenic formerization (enzyme treated bee milk), it is handled and the protein decomposition enzyme processing by royal jelly being carried out carbohydrate-splitting enzyme, makes anaphylaxis be reduced to unexpressed in fact degree (with reference to patent documentation 1).In addition, disclose in the patent documentation 2, the anaphylaxis of royal jelly is reduced by using the neutral peptase of endo-type to handle royal jelly.
But, handle the enzyme treated bee milk carry out hypoallergenic formerization by peptase, compare with the royal jelly of handling without enzyme, be subject to the influence of temperature or humidity, thus heat or exacting terms such as humidified condition under painted generation brownization.Like this painted, the Maillard reaction (Maillard Reaction) that is considered to take place between the anomeric carbon of the peptide of the fragmentation owing to enzymolysis or amino acid whose amide groups and reduced sugar produces.
Patent documentation 1: TOHKEMY 2002-112715 communique
Patent documentation 2: TOHKEMY 2005-287411 communique
Summary of the invention
The object of the present invention is to provide and a kind ofly contain the enzyme treated bee milk of handling through peptase and suppress painted enzyme treated bee milk composition simultaneously, particularly cryodesiccated enzyme treated bee milk composition.The object of the present invention is to provide a kind of manufacture method of described painted repressed enzyme treated bee milk composition in addition.
And the present invention also aims to provide a kind of inhibition enzyme treated bee milk method of colouring.
Painted substantially the do not become problem of the royal jelly of handling without enzyme when preserving or during freeze drying, but the enzyme treated bee milk after peptase is handled are subject to the influence of temperature or humidity, particularly exist when preserving or freeze drying causes painted problem.
Be not bound by theory, but the inventor has following consideration about paintedization of enzyme treated bee milk, the i.e. reduction of stability.
When peptase was handled royal jelly, the proteolysis that wherein contains generated amino acid or peptide.Between the amino of amino acid or peptide and the anomeric carbon of reduced sugar non-enzyme reaction takes place, promptly so-called Maillard reaction produces brown material.Maillard reaction takes place in food processing or when storage, painted, the generation of fragrance component with goods, the relevant reactions such as generation of non-oxidizability composition in food industry, and good aspect and bad aspect all will be paid attention to.Even known this Maillard reaction also carries out at normal temperatures, but can carry out at short notice by heating.In addition, known in Maillard reaction, pH, moisture (humidity) also become participation factors with heat.
The inventor is in order to address the above problem, promptly painted in order to prevent the enzyme treated bee milk after peptase is handled, carried out research repeatedly, found that by with enzyme treated bee milk with galactomannans, the polysaccharide that contains galactomannans, or the xanthans coexistence is placed down, can significantly prevent its through the time painted or that freeze drying causes is painted, particularly pass through at galactomannans enzyme treated bee milk, the polysaccharide that contains galactomannans, or carry out freeze drying under the xanthans existence, can significantly prevent this freeze drying thing through the time painted, thereby finished the present invention.
Comprise following embodiment among the present invention.
(I) enzyme treated bee milk composition
(I-1). a kind of enzyme treated bee milk composition, wherein, contain at least a kind and the enzyme treated bee milk after peptase is handled being selected from the group that is made of galactomannans, the polysaccharide that contains galactomannans and xanthans.
(I-2). the enzyme treated bee milk composition described in (I-1), wherein, enzyme treated bee milk is the royal jelly of the endopeptidase effect by peptase after being hydrolyzed.
(I-3). the enzyme treated bee milk composition described in (I-1), wherein, enzyme treated bee milk is the royal jelly after being hydrolyzed by the endopeptidase effect of peptase and two kinds of effects of expeptidase effect.
(I-4). as (I-1)～(I-3) each described enzyme treated bee milk composition, wherein, the described polysaccharide that contains galactomannans, for being selected from least a kind in the group that constitutes by locust bean gum, guar gum, tara gum and fenugreek gum, being preferably at least a kind that is selected from the group that constitutes by locust bean gum, tara gum and fenugreek gum.
(I-5). as (I-1)～(I-4) each described enzyme treated bee milk composition, wherein, described enzyme treated bee milk composition is the freeze drying thing.
(II) manufacture method of enzyme treated bee milk composition
(II-1). each described enzyme treated bee milk method for compositions of a kind of manufacturing (I-1)～(I-5), wherein, before the peptase of royal jelly is handled, add at least a kind that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans simultaneously or afterwards.
(II-2). the manufacture method described in (II-1), wherein, peptase is treated to the processing by the endopeptidase effect hydrolysis royal jelly of peptase.
(II-3). the manufacture method described in (II-1), wherein, peptase is treated to the processing by the endopeptidase effect of peptase and two kinds of effects of expeptidase effect hydrolysis royal jelly.
(II-4). as (II-1)～(II-3) each described manufacture method, wherein, the described polysaccharide that contains galactomannans, for being selected from least a kind in the group that constitutes by locust bean gum, guar gum, tara gum and fenugreek gum, being preferably at least a kind that is selected from the group that constitutes by locust bean gum, tara gum and fenugreek gum.
(II-5). the enzyme treated bee milk method for compositions described in a kind of manufacturing (I-5), wherein, in the enzyme treated bee milk after peptase is handled, add at least a kind that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans, and carry out freeze drying and handle.
(II-6). the manufacture method described in (II-5), wherein, enzyme treated bee milk is the royal jelly of the endopeptidase effect by peptase after being hydrolyzed.
(II-7). the manufacture method described in (II-5), wherein, enzyme treated bee milk is the royal jelly after being hydrolyzed by the endopeptidase effect of peptase and two kinds of effects of expeptidase effect.
(II-8). as (II-6) or the manufacture method (II-7), wherein, the described polysaccharide that contains galactomannans, for being selected from least a kind in the group that constitutes by locust bean gum, guar gum, tara gum and fenugreek gum, being preferably at least a kind that is selected from the group that constitutes by locust bean gum, tara gum and fenugreek gum.
(III) coloring inhibiting method of enzyme treated bee milk
(III-1). a kind of coloring inhibiting method of enzyme treated bee milk, wherein, before the peptase of royal jelly is handled, add at least a kind that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans simultaneously or afterwards, with enzyme treated bee milk with the described at least a kind of coexistence that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans under place.
(III-2). the coloring inhibiting method described in (III-1), wherein, described peptase is treated to the processing by the endopeptidase effect hydrolysis royal jelly of peptase.
(III-3). the coloring inhibiting method described in (III-1), wherein, described peptase is treated to the processing by the endopeptidase effect of peptase and two kinds of effects of expeptidase effect hydrolysis royal jelly.
(III-4). as (III-1)～(III-3) each described coloring inhibiting method, wherein, the described polysaccharide that contains galactomannans, for being selected from least a kind in the group that constitutes by locust bean gum, guar gum, tara gum and fenugreek gum, being preferably at least a kind that is selected from the group that constitutes by locust bean gum, tara gum and fenugreek gum.
(III-5). a kind of coloring inhibiting method of enzyme treated bee milk, wherein, in the enzyme treated bee milk after peptase is handled, add at least a kind that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans, and under enzyme treated bee milk and the described at least a kind of coexistence that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans, carry out freeze drying and handle.
(III-6). the coloring inhibiting method described in (III-5), wherein, described enzyme treated bee milk is the royal jelly of the endopeptidase effect by peptase after being hydrolyzed.
(III-7). the coloring inhibiting method described in (III-5), wherein, described enzyme treated bee milk is the royal jelly after being hydrolyzed by the endopeptidase effect of peptase and two kinds of effects of expeptidase effect.
(III-8). as (III-6) or the coloring inhibiting method (III-7), wherein, the described polysaccharide that contains galactomannans, for being selected from least a kind in the group that constitutes by locust bean gum, guar gum, tara gum and fenugreek gum, being preferably at least a kind that is selected from the group that constitutes by locust bean gum, tara gum and fenugreek gum.
According to the present invention, can provide a kind of enzyme treated bee milk composition with and manufacture method, described enzyme treated bee milk composition, by reducing the raising that allergic reaction realizes security, suppress simultaneously by peptase decompose the stability that the amino that produces and carboxyl cause reduce, particularly the amino Maillard reaction that causes produce painted.
In addition according to the present invention, about handling and the enzyme treated bee milk of hypoallergenic formerization by peptase, can significantly suppress it since processing such as freeze drying cause painted or preservation causes through the time painted.
Description of drawings
Fig. 1 is expression about the enzyme treated bee milk (no interpolation group 2) of the enzyme treated bee milk composition (embodiment 2 compositions) of embodiment 2 and comparative example 2 " brightness reduction (the Δ L after preservation (40 ℃, 54%RH, 24 hours)
*) " figure (experimental example 2).
Fig. 2 is expression about embodiment 2 compositions and no interpolation group 2 " red recruitment (the Δ a after preservation (40 ℃, 54%RH, 24 hours)
*) " figure (experimental example 2).
Fig. 3 is expression about embodiment 2 compositions and no interpolation group 2 " yellow recruitment (the Δ b after preservation (40 ℃, 54%RH, 24 hours)
*) " figure (experimental example 2).
Fig. 4 is about the composition of embodiment 2,4,6,8 and comparative example 2 and 3 " brightness reduction (the Δ L after preserving (40 ℃, 54%RH, 24 hours)
*) " comparison diagram (experimental example 2).
Fig. 5 is about the composition of embodiment 2,4,6,8 and comparative example 2 and 3 " red recruitment (the Δ a after preserving (40 ℃, 54%RH, 24 hours)
*) " comparison diagram (experimental example 2).
Fig. 6 is about the composition of embodiment 2,4,6,8 and comparative example 2 and 3 " yellow recruitment (the Δ b after preserving (40 ℃, 54%RH, 24 hours)
*) " comparison diagram (experimental example 2).
The specific embodiment
(1) enzyme treated bee milk composition and manufacture method thereof
Enzyme treated bee milk composition of the present invention is characterised in that, contains at least a kind and the enzyme treated bee milk after peptase is handled being selected from the group that is made of galactomannans, the polysaccharide that contains galactomannans and xanthans.This enzyme treated bee milk composition can suppress the generation of enzyme treated bee milk toffee material, thus the carrying out of control brownization, on this meaning through the time stable.
Royal jelly is that 3～12 days worker bee of age in days in the honeybee is mixed and the milky colloid substance that is made into from the secretion of hypopharynx cephalic gland and big jaw glandular secretion.As the major physiological active component in the royal jelly, for example, can enumerate protein based on organic acids such as distinctive 10-hydroxyl caproleic acids in the royal jelly (below be designated as " decylenic acid "), lipid, carbohydrate, vitamins such as vitamin(e) B group, folic acid, nicotinic acid, pantothenic acid, various mineral matter classes etc.As the physiologically active or the pharmacological action of this royal jelly, known antibacterial action arranged, strengthen immunization, antidepressant effect, antitumor action, antiinflammatory action, the effect of increase CBF etc.In addition, the side effect of reduction anticarcinogen and the effect of the long-living life of radioactive ray injury time delay have also been reported.
As making the royal jelly that uses in the enzyme treated bee milk, without limits, can use fresh royal jelly or make the dry also royal jelly powder of powdered of fresh royal jelly.The place of production of royal jelly can be any one in Japan, China, Brazil, European various countries, various countries, Oceania, the U.S. etc. without limits.
As the enzyme treated bee milk of object of the present invention, be the material that obtains with protein decomposition enzyme (peptase) processing royal jelly.Preferably, peptase is suppressed the hypoallergenic formerization enzyme treated bee milk that forms because handling the caused allergic reaction of protein that contains in the royal jelly.Therefore, in the enzyme treated bee milk of the present invention, the peptase analyte of the protein that in royal jelly, contains, can contain above-mentioned organic acids such as decylenic acid, lipid, carbohydrate, vitamins and various mineral matter class.
As the enzyme that royal jelly is carried out use in the enzyme processing, can preferably enumerate peptase.The peptase that uses, as long as have at least a of endopeptidase effect and expeptidase effect, but the preferred peptase that has the endopeptidase effect at least, more preferably these two kinds of peptases that effect all has.
Enzyme treated bee milk of the present invention, the protein that contains in the selective hydrolysis royal jelly and the material of hypoallergenic formerization.Therefore, preferably, use the peptase (endopeptidase) that has the endopeptidase effect at least, the preferred peptide enzyme hydrolysis royal jelly that uses with endopeptidase effect and two kinds of effects of expeptidase effect.At this, as peptase with endopeptidase effect and two kinds of effects of expeptidase effect, can use the peptase that has these two kinds of effects simultaneously separately, perhaps also can make up peptase (endopeptidase) and the peptase (expeptidase) with expeptidase effect used with endopeptidase effect.
As operable endopeptidase among the present invention,, then can be any if having the protein decomposition enzyme of endopeptidase activity at least.For example extensively illustration from animal (for example, trypsase, chymotrypsin etc.), from plant (for example, papain etc.) or from the endopeptidases of microorganism (for example, lactic acid bacteria, yeast, mould, withered grass bacterium, actinomyces etc.).
As expeptidase,, then can be any if having the protein decomposition enzyme of expeptidase activity at least.Can illustration for example carboxypeptidase, aminopeptidase or from the expeptidase of microorganism (for example, lactic acid bacteria, aspergillus bacterium, rhizopus) or also have pancreatin, pepsin of endopeptidase activity etc. concurrently.
In fact only have the expeptidase of expeptidase effect, in fact only have the endopeptidase of endopeptidase effect and have the expeptidase effect and the peptase of two kinds of effects of endopeptidase effect but exist in the peptase.The enzyme that has expeptidase effect and two kinds of effects of endopeptidase effect in these, when the endopeptidase effect is strong, can be used as " endopeptidase " uses, when the expeptidase effect is strong, can be used as " expeptidase " uses, when expeptidase effect and endopeptidase effect quite or almost quite the time, can be used as the peptase that has endopeptidase effect and expeptidase effect simultaneously and use.
In such peptase, preferred example as peptase with expeptidase effect and two kinds of effects of endopeptidase effect, for example can enumerate: the peptase (trade name: ア Network チ Na one ゼ AS) that streptomyces griseus (Streptomycesgriseus) produces, peptase (the trade name: プ ロ テ ア one ゼ A that aspergillus oryzae (Aspergillusorizae) produces, Off レ one バ one ザ イ system), peptase (the trade name: プ ロ テ ア one ゼ P) that honey aspergillus (Aspergillus melleus) produces, in addition, as the preferred example of enzyme, for example can enumerate: the peptase (trade name: ウ マ ミ ザ イ system G that aspergillus oryzae (Aspergillus orizae) produces with expeptidase effect, Promod192P, Promod 194P, ス ミ チ one system FLAP), peptase (the trade name: Sternzyme B15024) that Aspergillus sojae (Aspergillus sojae) produces, peptase (the trade name: コ Network ラ one ゼ P) that aspergillus produces, peptase (the trade name: ペ プ チ ダ one ゼ R) that Rhizopus oryzae (Rhizopus oryzae) produces.
As the preferred example of peptase, for example can enumerate: the peptase (trade name: オ リ エ Application one ゼ 22BF that hay bacillus (Bacillus subtilis) produces with endopeptidase effect, ヌ Network レ イ シ Application), peptase (the trade name: ア Le カ ラ one ゼ) that bacillus licheniformis (Bacillus licheniformis) produces, peptase (the trade name: プ ロ テ ア one ゼ S) that bacillus stearothermophilus (Bacillus stearothermophilus) produces, peptase (the trade name: ニ ユ one ト ラ one ゼ) that bacillus amyloliquefaciens (Bacillusamyloliquefaciens) produces, peptase (the trade name: プ ロ メ Star Network ス) that bacillus produces.
With respect to the use amount of the peptase of royal jelly, according to the royal jelly concentration, the enzyme that use tire, reaction temperature and reaction time and difference, but the every 1g of protein that contains in the royal jelly usually is preferably with the ratios use peptase of 50～10000 effect units.In addition, at this moment, peptase adds in the royal jelly, can once add, and also gradation is added on a small quantity.
The pH of royal jelly when peptase is handled, the corresponding optimal pH that uses enzyme is from pH2～12, preferred pH7.5～10, more preferably the scope of pH7.8～9 is selected.Particularly, in above-mentioned royal jelly, add peptase before, according to the kind of using enzyme, by adding the pH that acid, alkali or buffer are adjusted to expectation, with reach pH2～12, preferred pH7.5～10, more preferably in the scope of pH7.8～9.At this moment, as acid, can illustration hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, acetic acid etc.; As alkali, can illustration NaOH, potassium hydroxide, potash etc.; In addition as buffer, can the illustration phosphoric acid buffer agent, citric acid buffer agent etc.
The temperature that peptase is handled is not particularly limited, can from can be for the scope of practical application, promptly be generally 30～70 ℃ the scope and select, this scope comprise the peptase effect that shows, preferably show the endopeptidase effect, more preferably show the optimum temperature range of endopeptidase effect and two kinds of effects of expeptidase effect.In the temperature low or higher, for example in 50～60 ℃ the scope, can prevent addling in the peptase processing procedure by holding temperature than the optimum temperature of peptase (preferred about 40～about 50 ℃).The time that peptase is handled depends on reaction conditions such as the kind of using enzyme, reaction temperature, pH, is not particularly limited.
What peptase was handled stops, by making the peptide enzyme deactivation or removing peptase and carry out.Inactivation operation can be passed through heat treated (for example, 85 ℃, 15 minutes etc.) and carry out.
Enzyme treated bee milk among the present invention, it can be the foregoing at least royal jelly of handling through peptase, also comprise not only through peptase handle and through with the royal jelly of the combined treatment of other enzymes, for example when peptase is handled, carry out the royal jelly that carbohydrate-splitting enzyme is handled.
The galactomannans that uses among the present invention, broad sense are meant the polysaccharide that is made of D-galactolipin and D-mannose.Particularly, galactomannans has the structure of α-1,6 key at the last galactose residue of the main chain (mannosan) of the mannose residue of β-1,4 key as side chain.The ratio of mannose residue and galactose residue is not particularly limited.
In addition, among the present invention, can use the polysaccharide that contains galactomannans to replace galactomannans, or be used in combination the polysaccharide that contains galactomannans with it.As this polysaccharide, preferably illustration is the polysaccharide of main component with the galactomannans, is specially locust bean gum, guar gum, tara gum and fenugreek gum etc., preferred locust bean gum, tara gum and fenugreek gum, more preferably locust bean gum.These polysaccharides can use separately or be used in combination arbitrarily more than 2 kinds.
In the polysaccharide that contains galactomannans, except galactomannans and constitute sugared D-galactolipin and the D-mannose, comprise and contain for example other sugared polysaccharides such as D-glucose as object of the present invention.Contain above-mentioned galactomannans with about ratio more than 50% this moment, as remaining composition, can be the polysaccharide that comprises D-galactolipin and D-mannose and sugar in addition thereof.
In addition, among the present invention, can use xanthans to replace above-mentioned galactomannans or contain the polysaccharide of galactomannans, perhaps be used in combination xanthans with galactomannans or at least a kind of polysaccharide that contains galactomannans.
The ratio of the above-mentioned galactomannans that contains in the enzyme treated bee milk composition of the present invention, the polysaccharide that contains galactomannans and xanthans is, per 100 weight portions of the enzyme treated bee milk that contains in the enzyme treated bee milk composition, the total amount of the above-mentioned material that contains is generally about 1～about 20 weight portions, preferred about 2～about 10 weight portions, more preferably from about 2～about 5 weight portions.When the combined amount of these compositions was very few, the stability of enzyme treated bee milk, particularly painted inhibition descended.
Enzyme treated bee milk composition of the present invention can be by handling front and back at the peptase of royal jelly or adding above-mentioned at least a kind of being selected from the group that is made of galactomannans, the polysaccharide that contains galactomannans and xanthans simultaneously and make.Preferably by handling afterwards at the peptase of royal jelly, promptly in enzyme treated bee milk, adding to mix and make.
Enzyme treated bee milk composition of the present invention, its form is not particularly limited, and can be that liquid condition also can be a solid state, but preferably have freeze-dried.This freeze drying thing can by before and after the peptase of royal jelly is handled or add simultaneously mix above-mentioned be selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans at least a kind, preferably add in the enzyme treated bee milk after peptase is handled and mix, use it for freeze drying afterwards and handle and prepare.Need to prove that freeze drying processing method is according to the rules carried out.
Zhi Bei enzyme treated bee milk composition of the present invention like this, can be the form of liquid dosage forms such as health drink, syrup, also can make it become forms such as freeze drying thing (for example freeze-dried powder) form or the tablet of making to wherein adding proper additive, chewable tablets, capsule, particle.
(2) coloring inhibiting method of enzyme treated bee milk
The coloring inhibiting method of enzyme treated bee milk of the present invention can followingly be implemented: before the peptase of royal jelly is handled, simultaneously or add above-mentioned at least a kind of being selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans afterwards, with enzyme treated bee milk with the above-mentioned at least a kind of coexistence that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans under place.
At this, about galactomannans, the polysaccharide that contains galactomannans and the xanthans of handling and using as the peptase kind or the peptase of the royal jelly of object, use, described in (1).
In addition, the coloring inhibiting method of enzyme treated bee milk, also can followingly implement: in the enzyme treated bee milk after peptase is handled, add at least a kind that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans, and make enzyme treated bee milk with the above-mentioned at least a kind of coexistence that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans under carry out freeze drying and handle.By the freeze drying thing that this method obtains, be to contain preferred enzyme treated bee milk composition among the present invention of enzyme treated bee milk of painted holddown.Freeze drying is handled and also can be implemented according to method described in (1).
Embodiment
Below, describe the present invention in detail according to embodiment and experimental example.But the present invention is not limited to these embodiment.
The fresh royal jelly 200g of measuring China's production adds ion exchange water 100mL in the 500mL beaker, be stirred to evenly preparation royal jelly dilution.To the NaOH aqueous solution that wherein adds 2N, the pH that adjusts the royal jelly dilution is 8.7～8.9.The solution that ア Network チ Na one ゼ AS (scientific research pharmacy) 2g is dissolved in behind the ion exchange water 20mL joins in the above-mentioned royal jelly dilution afterwards, and adding ion exchange water again, to make the ion-exchange water inventory be 200mL.Use the propeller agitation reactant mixture, reacted 4 hours down at 50 ℃ (Water Tanks with Temp.-controlled) simultaneously, be hydrolyzed.The temperature to 80 of Water Tank with Temp.-controlled of raising afterwards ℃ makes enzyme deactivation, cools off in water then, obtains enzyme treated bee milk (solution).According to prescription shown below, in this enzyme treated bee milk 50g (solid conversion), add locust bean gum 2g and avicel cellulose 4g, be stirred to evenly.With its freeze drying, obtain enzyme treated bee milk composition (freeze drying thing).
(prescription)
Enzyme treated bee milk 50g
Locust bean gum 2g
Avicel cellulose 4g
The fresh royal jelly 500g of measuring China's production adds ion exchange water 250mL in the 2000mL beaker, be stirred to evenly preparation royal jelly dilution.To the NaOH aqueous solution that wherein adds 2N, the pH that adjusts the royal jelly dilution is 8.6～8.9.The solution that ア Network チ Na one ゼ AS (scientific research pharmacy) 5g is dissolved in behind the ion exchange water 50mL joins in the above-mentioned royal jelly dilution afterwards, and adding ion exchange water again, to make the ion-exchange water inventory be 500mL.Use the propeller agitation reactant mixture, reacted 4 hours down at 50 ℃ (Water Tanks with Temp.-controlled) simultaneously, be hydrolyzed.The temperature to 80 of Water Tank with Temp.-controlled of raising afterwards ℃ makes enzyme deactivation, cools off in water then, obtains enzyme treated bee milk (solution).Measure this enzyme treated bee milk 100mL in the 300mL beaker,, add locust bean gum 0.64g and avicel cellulose 1.27g therein, be stirred to evenly according to prescription shown below.With its freeze drying, obtain enzyme treated bee milk composition (freeze drying thing).
(prescription)
Enzyme treated bee milk 100mL
Locust bean gum 0.64g
Avicel cellulose 1.27g
Handle fresh royal jelly similarly to Example 1, in the enzyme treated bee milk 50g that obtains (solid conversion), use xanthans 2g to replace locust bean gum, operation similarly to Example 1, preparation enzyme treated bee milk composition (freeze drying thing).
Handle fresh royal jelly similarly to Example 2, in the enzyme treated bee milk that obtains (solution) 100mL, use xanthans 0.64g to replace locust bean gum, operation similarly to Example 2, preparation enzyme treated bee milk composition (freeze drying thing).
Embodiment 5
Handle fresh royal jelly similarly to Example 1, in the enzyme treated bee milk 50g that obtains (solid conversion), use tara gum 2g to replace locust bean gum, operation similarly to Example 1, preparation enzyme treated bee milk composition (freeze drying thing).
Handle fresh royal jelly similarly to Example 2, in the enzyme treated bee milk that obtains (solution) 100mL, use tara gum 0.64g to replace locust bean gum, operation similarly to Example 2, preparation enzyme treated bee milk composition (freeze drying thing).
Handle fresh royal jelly similarly to Example 1, in the enzyme treated bee milk 50g that obtains (solid conversion), use fenugreek gum 2g to replace locust bean gum, operation similarly to Example 1, preparation enzyme treated bee milk composition (freeze drying thing).
Handle fresh royal jelly similarly to Example 2, in the enzyme treated bee milk that obtains (solution) 100mL, use fenugreek gum 0.64g to replace locust bean gum, operation similarly to Example 2, preparation enzyme treated bee milk composition (freeze drying thing).
Comparative example 1
Handle fresh royal jelly similarly to Example 1, in the enzyme treated bee milk 50g that obtains (solid conversion), do not add locust bean gum and avicel cellulose, carry out freeze drying similarly to Example 1 and handle, preparation enzyme treated bee milk (freeze drying thing) (below be referred to as " no interpolation group 1 ").
Comparative example 2
Handle fresh royal jelly similarly to Example 2, in the enzyme treated bee milk that obtains (solution) 100mL, do not add locust bean gum and avicel cellulose, carry out freeze drying similarly to Example 2 and handle, preparation enzyme treated bee milk (freeze drying thing) (below be referred to as " no interpolation group 2 ").
Comparative example 3
Handle fresh royal jelly similarly to Example 2, in the enzyme treated bee milk that obtains (solution) 100mL, do not add locust bean gum, only add avicel cellulose 1.27g, carry out freeze drying similarly to Example 2 and handle, preparation enzyme treated bee milk composition (freeze drying thing) (below be referred to as " cellulose group ").
Experimental example 1
With the enzyme treated bee milk (freeze drying thing) (no interpolation group 1) for preparing in the enzyme treated bee milk composition (freeze drying product) of adding locust bean gum of preparation among the embodiment 1 and the comparative example 1, under " 37 ℃, air tight condition " and " 37 ℃, humidity 75%RH condition " two kinds of conditions, placed 28 days respectively, by visual through the time observe painted degree, estimate stability (painted inhibition).Need to prove that in " 37 ℃, air tight condition ", the sample bottle cap of confining zone screw-cap becomes airtight conditions, adjusts the temperature to 37 ℃, in addition, in " 37 ℃, humidity 75%RH condition ", utilize Constant Temperature and Humidity Chambers to regulate.
The result is as shown in table 1.As shown in table 1, its result is clear and definite to make it become the enzyme treated bee milk composition that adds locust bean gum by mix locust bean gum in enzyme treated bee milk, can suppress the painted of enzyme treated bee milk.
| 37 ℃, air tight condition | 37℃、75％RH | |||
| Enzyme is handled RJ (no interpolation group 1) | Enzyme is handled RJ composition (embodiment 1) | Enzyme is handled RJ (no interpolation group 1) | Enzyme is handled RJ composition (embodiment 1) | |
| The 0th day | - | - | - | - |
| The 7th day | - | - | ++ | + |
| The 14th day | ± | - | +++ | + |
| The 28th day | + | - | ++++ | ++ |
RJ: royal jelly
-: milky, ±: milk yellow ,+: tenne, ++: brown, +++: pitchy, ++ ++: black
The color that will show above-mentioned degree of staining, by be widely used in printing industry, when estimating with the colorimeter of colour chart (CMYK figure) based on printing, as described below.
Color indicia is carried out for 4 types with blue-green (C), carmetta (M), yellow (Y), black (BL), expression site concentration ratio (%) separately.
-(milky): C0+M0+Y20+BL0～C0+M5+Y20+BL0
± (milk yellow): C0+M20+Y50+BL20
+ (tenne): C0+M30+Y50+BL10～C0+M40+Y70+BL10
++ (brown): C0+M60+Y50+BL20～C0+M60+Y70+BL20
+++(pitchy): C70+M70+Y50+BL30
++ ++ (black): C100+M100+Y50+BL30
Experimental example 2
With the enzyme treated bee milk composition (cellulose group) for preparing in the enzyme treated bee milk (no interpolation group 2) of preparation in the enzyme treated bee milk composition, comparative example 2 of preparation in embodiment 2,4,6 and 8 and the comparative example 3 (more than be the freeze drying thing), be placed on according to following method under the condition of " 40 ℃, humidity 54%RH " of setting, measure the tone of above-mentioned each freeze drying thing after 24 hours, judge the variation of tone, estimate stability (painted inhibition) thus.
＜40 ℃, humidity 54%RH condition 〉
With adding the saturated solution that purified water 100mL makes in the sodium bromide (150g), be encased in the desiccator, desiccator is put into 40 ℃ thermostat and heated 48 hours.Humidity uses simple and easy Hygrothermograph to be adjusted to 54%RH.
The tone of each freeze drying thing is measured and is judged, by carrying out with lower device, condition determination and method.
(1) determinator
UV, visible light extinction photometer: Japanese beam split V-660
Integrating sphere device: Japanese beam split PSC-718
Use software: Japanese beam split color calculation procedure
(2) condition determination
Light source: deuterium lamp, halogen lamp
Measure wavelength: 800-200nm
Data break: 1nm
(3) determination methods of tone variations
(180～800nm) measure, and are converted to L with bundled software (color calculation procedure) to carry out the ultraviolet spectrum with UV, visible light extinction photometer (Japanese beam split V-660+ integrating sphere device PSC-718) under the pulverulence
*a
*b
*Color specification system (with reference to the source JISZ8729-2004 of calculating formula).To brightness reduction (the Δ L that obtains like this
*), red recruitment (Δ a
*) and yellow recruitment (Δ b
*) 3 key elements, judge tone variations.Calculate painted inhibiting rate (%) according to following formula, carry out the judgement of tone variations.
Painted inhibiting rate (%)=[1-(value of enzyme treated bee milk composition)/(value of no interpolation group 2)] * 100
Result about the enzyme treated bee milk composition of embodiment 2 and the enzyme treated bee milk of comparative example 2 (no interpolation group 2) is as shown in table 2, and is as shown in table 3 about the result of the enzyme treated bee milk (no interpolation group 2) of the enzyme treated bee milk composition (cellulose group) of comparative example 1 and comparative example 2.In addition about table 2, brightness reduction (Δ L
*), red recruitment (Δ a
*) and yellow recruitment (Δ b
*) 3 key elements map respectively, as Fig. 1, Fig. 2 and shown in Figure 3.Brightness reduction (Δ L
*), red recruitment (Δ a
*) and yellow recruitment (Δ b
*) all little, mean and suppressed painted.
Table 2
| |
No |
Painted inhibiting rate (%) | |
| Brightness reduction (Δ L *) | 19.03 | 30.55 | 37.7 |
| Red recruitment (Δ a *) | 3.19 | 6.15 | 48.1 |
| Yellow recruitment (Δ b *) | 11.33 | 13.91 | 18.5 |
Table 3
| The cellulose group | No |
Painted inhibiting rate (%) | |
| Brightness reduction (Δ L *) | 33.06 | 30.55 | -8.22 |
| Red recruitment (Δ a *) | 7.93 | 6.15 | -28.94 |
| Yellow recruitment (Δ b *) | 12.56 | 13.91 | 9.71 |
By these results clear and definite in enzyme treated bee milk, mix locust bean gum and make it become the enzyme treated bee milk composition that adds locust bean gum, can suppress the painted of enzyme treated bee milk.
In addition, about the enzyme treated bee milk composition (cellulose group) for preparing in the enzyme treated bee milk (no interpolation group 2) of preparation in the enzyme treated bee milk composition, comparative example 2 of preparation in embodiment 2,4,6 and 8 and the comparative example 3 (more than be the freeze drying product), by brightness reduction (Δ L
*), red recruitment (Δ a
*) and yellow recruitment (Δ b
*) 3 key elements, calculate aberration (Δ E according to following formula
*Ab), obtain painted inhibiting rate (%), estimate tone variations (painted inhibiting rate) by this aberration.
Aberration (Δ E
*Ab)=[(Δ L
*)
2+ (Δ a
*)
2+ (Δ b
*)
2]
1/2
Painted inhibiting rate (%)=[1-(aberration of enzyme treated bee milk composition)/(aberration of no interpolation group 2)] * 100
The result is shown in table 4, Fig. 4～6.
Table 4
| Embodiment/comparative example | Brightness reduction (Δ L *) | Red recruitment (Δ a *) | Yellow recruitment (Δ b *) | Aberration (Δ E*ab) | Inhibiting rate (%) |
| Embodiment 2: locust bean gum | 19.03 | 3.19 | 11.33 | 22.38 | 34.43 |
| Embodiment 4: xanthans | 22.64 | 5.17 | 13.97 | 27.10 | 20.60 |
| Embodiment 6: tara gum | 25.27 | 4.15 | 11.42 | 28.04 | 17.84 |
| Embodiment 8: fenugreek gum | 25.48 | 2.69 | 11.18 | 27.95 | 18.11 |
| Comparative example 3: cellulose group | 33.06 | 7.93 | 12.56 | 36.24 | -6.21 |
| Comparative example 2: no |
30.55 | 6.15 | 13.91 | 34.13 | - |
By these results clear and definite in enzyme treated bee milk, mix polysaccharide that contains galactomannans or the xanthans be called as locust bean gum, tara gum and fenugreek gum, make it become the enzyme treated bee milk composition that adds polysaccharide, can suppress the painted of enzyme treated bee milk.In addition, by this result as can be known, be called as the polysaccharide that contains galactomannans of locust bean gum, tara gum and fenugreek gum and xanthans for suppressing the painted effectively same of enzyme treated bee milk, that is, galactomannans and xanthans for suppress enzyme treated bee milk painted effectively.
Claims (8)
1. an enzyme treated bee milk composition wherein, contains at least a kind and the enzyme treated bee milk after peptase is handled being selected from the group that is made of galactomannans, the polysaccharide that contains galactomannans and xanthans.
2. the enzyme treated bee milk composition described in claim 1, wherein, enzyme treated bee milk is the royal jelly after being hydrolyzed by endopeptidase effect and two kinds of effects of expeptidase effect.
3. the enzyme treated bee milk composition described in claim 1 or 2, wherein, the described polysaccharide that contains galactomannans is for being selected from least a kind in the group that is made of locust bean gum, tara gum and fenugreek gum.
4. as each described enzyme treated bee milk composition of claim 1～3, wherein, described composition is the freeze drying thing.
5. make each described enzyme treated bee milk method for compositions of claim 1～4 for one kind, wherein, before the peptase of royal jelly is handled, add at least a kind that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans simultaneously or afterwards.
6. make the enzyme treated bee milk method for compositions described in the claim 4 for one kind, wherein, in the enzyme treated bee milk after peptase is handled, add at least a kind that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans, and carry out freeze drying and handle.
7. the coloring inhibiting method of an enzyme treated bee milk, wherein, before the peptase of royal jelly is handled, add at least a kind that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans simultaneously or afterwards, with enzyme treated bee milk with the described at least a kind of coexistence that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans under place.
8. the coloring inhibiting method of an enzyme treated bee milk, wherein, in the enzyme treated bee milk after peptase is handled, add at least a kind that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans, and under enzyme treated bee milk and the described a kind of coexistence that is selected from the group that constitutes by galactomannans, the polysaccharide that contains galactomannans and xanthans, carry out freeze drying at least and handle.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250233A (en) * | 2011-07-05 | 2011-11-23 | 北京师范大学 | Royal jelly polypeptide and application thereof |
| CN102251005A (en) * | 2011-07-05 | 2011-11-23 | 北京师范大学 | Preparation method of royal jelly polypeptide |
| CN106620746A (en) * | 2015-11-02 | 2017-05-10 | 中国石油化工股份有限公司 | Method used for reducing inhibition effect of Maillard reaction on microorganism growth and fermentation |
| CN110582289A (en) * | 2017-03-16 | 2019-12-17 | 株式会社山田养蜂场本社 | Enhancer of genome stability |
| CN111902052A (en) * | 2018-03-28 | 2020-11-06 | 株式会社山田养蜂场本社 | Stabilization of enzymatically decomposed royal jelly |
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| TW201336429A (en) * | 2012-03-03 | 2013-09-16 | Vitalon Foods Co Ltd | Royal jelly solution and manufacturing method thereof |
| JP6985666B2 (en) * | 2016-12-27 | 2021-12-22 | アピ株式会社 | Manufacturing method of royal jelly material, royal jelly material, food and drink containing royal jelly, and cosmetics containing royal jelly |
| CN110075128A (en) * | 2018-01-26 | 2019-08-02 | 拜特克有限公司 | A kind of pharmaceutical compositions for being used to enhance immune function comprising enzyme treated bee milk powder as effective component |
| WO2019221078A1 (en) * | 2018-05-18 | 2019-11-21 | 株式会社山田養蜂場本社 | Stabilization of enzyme-degraded royal jelly |
| WO2020196065A1 (en) * | 2019-03-26 | 2020-10-01 | 株式会社山田養蜂場本社 | Method for producing royal jelly enzymatic decomposition product, and royal jelly enzymatic decomposition product |
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2008
- 2008-01-10 JP JP2008002747A patent/JP4182366B2/en active Active
- 2008-01-17 CN CN2008100034712A patent/CN101223946B/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250233A (en) * | 2011-07-05 | 2011-11-23 | 北京师范大学 | Royal jelly polypeptide and application thereof |
| CN102251005A (en) * | 2011-07-05 | 2011-11-23 | 北京师范大学 | Preparation method of royal jelly polypeptide |
| CN102251005B (en) * | 2011-07-05 | 2013-06-19 | 北京师范大学 | Preparation method of royal jelly polypeptide |
| CN106620746A (en) * | 2015-11-02 | 2017-05-10 | 中国石油化工股份有限公司 | Method used for reducing inhibition effect of Maillard reaction on microorganism growth and fermentation |
| CN106620746B (en) * | 2015-11-02 | 2019-06-14 | 中国石油化工股份有限公司 | A method of it reducing Maillard reaction and microorganism growth and fermentation inhibitory is acted on |
| CN110582289A (en) * | 2017-03-16 | 2019-12-17 | 株式会社山田养蜂场本社 | Enhancer of genome stability |
| CN111902052A (en) * | 2018-03-28 | 2020-11-06 | 株式会社山田养蜂场本社 | Stabilization of enzymatically decomposed royal jelly |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2008194031A (en) | 2008-08-28 |
| CN101223946B (en) | 2011-11-16 |
| JP4182366B2 (en) | 2008-11-19 |
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