CN101223855A - Method for predicting differentiation ability of rubber tree secondary milk tube in seedling stage - Google Patents
Method for predicting differentiation ability of rubber tree secondary milk tube in seedling stage Download PDFInfo
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- CN101223855A CN101223855A CNA2008100033527A CN200810003352A CN101223855A CN 101223855 A CN101223855 A CN 101223855A CN A2008100033527 A CNA2008100033527 A CN A2008100033527A CN 200810003352 A CN200810003352 A CN 200810003352A CN 101223855 A CN101223855 A CN 101223855A
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Abstract
The invention relates to a method for predicting the differentiation capacity of the secondary milk vessels of rubber plants in a seedling stage which is characterized in following aspects: a) in October and November every year, sawing the branches of the rubber plant seeds that are stored in a seedling nursery, leading residue stumps to sprout branches which are stump shoots, and selecting the newborn stump shoots with 3 to 5 stretch units as the material; b) mechanically injuring a stem which lies in the first stretch unit of the stump shoot and has scale leaves; c) the mechanical injury processing is implemented in the season between March - July, and the processing day is sunny; d) the stump shoot biotemperature is the time when the leaves of the first stretch unit grow ripe and terminal bud lies in an inactive state. The mechanical injury method uses a single-side or double-side knife for scraping the epidermis and part of the cortical layers, and the scraped surfaces are directly exposed in air for 2 to 10 hours. The invention has the advantages that the invention well predicts the differentiation capacity of secondary milk vessels of Amazon wild species and Wickham species in the seedling stage.
Description
Technical field
The invention belongs to the method for predicting differentiation ability of rubber tree secondary milk tube a kind of seedling stage.
Background technology
Para rubber tree is a kind of woody economic crops of cross pollination height heterozygosis, traditional crossbreeding cycle is long, begin generally to need for 30 years for the kind that produce to go up the large-scale promotion plantation from the artificial pollination seedling to selecting 1, also need expend a large amount of soils and labour (Venkatachal am P.Priya P.Amma therebetween, C.K.S.Thulaseedharan, A.Identification, cloning and sequence analysisof a dwarf genome-specific RAPD marker in rubber tree (Hevea brasiliensisMuell.Arg.Plant Cell Rep.2004,23,327-332.).For shortening the breeding cycle, improve seed selection efficient, reduce the waste of soil, human and material resources, the research that Jiao Guodou pays much attention to the output method for early prediction is respectively planted in the world, reported the multiple method that is used for the output early prediction (Zhou Zhong gives birth. external Para rubber tree output method for early prediction research overview. the tropical agriculture science, 1993, the 2 phases, 77-83; Guo Qiyan, Zhou Zhongyu, Zhan Sairong. bamboo grows output early prediction is assessment report (1982-1994) for the second time. the tropical crop journal, 1995,15:9-13.).These output method for early prediction can be divided into two classes: a class is directly to detect output seedling stage, and a class is to detect other factor relevant with output seedling stage.The method that directly detects output seedling stage has vein glue method and the petiolule glue method and external thorn inspection method, test tapping method and the petiole gel content determination method of China.Detect seedling stage other factor relevant with output comprise rubber particles quantity, the physiological parameter (as Phos, mercaptan, pH value, cane sugar content, rupture index and silt index etc.) of latex, vein in growing situation (stem girth), latex dust columns, latex dust diameter, screen casing diameter, pore quantity, the latex dust examine and determine angle, cotyledon oil content, to the physiological parameter-acc oxidase of the reaction of output stimulant-ethrel and the physiological parameter-PSII activity of photosynthetic efficiency etc.
Bamboo grows output early prediction will reach the purpose of " early ", " province " and " standard "." early " and " province " accompany, the time of prediction is saved soil and manpower more more.Vein glue method and petiolule glue method have the advantage of " early " and " province ", but for the shortcoming (Ceng Xia that predicts that Amazon wild rubber seeds matter has not " standard ", Hu Yanshi, Huang Huasun, appraisal report---field planting material in 1987 of Fan family woods .1981 ' IRRDB bamboo grows germplasm. the tropical crop journal, 2006,27:34-38.).Its reason of censuring be the latex that detects from the nascent latex dust in the blade, can only reflect the product glue ability of nascent latex dust.In the bamboo grows process of growth, original nascent latex dust is finally squeezed and is ruined gradually to the outer passing of bark.Therefore, nascent latex dust in the trunk bark that becomes the bamboo grows in age, only from the secondary latex dust of vascular cambium, and such latex dust is only and the closely-related latex dust of natural rubber output.Thorn inspection method also has the advantage of " early ", " province " and the shortcoming of " standard " not.Though the position of its detection is at the stem of plant, but, because plant has only 12-18 monthly age, the position of secondary latex dust differentiation is few in stem, nascent latex dust also accounts for quite great proportion (HaoB.-Z.Wu J.-L.Laticifer differentiation in Hevea brasiliensis:Inducedby exogenous jasmonic acid and linolenic acid.Ann.Bot.2000,85:37-43.), so, the same with vein glue method with petiolule glue method, the latex that thorn inspection method is detected is also mainly from nascent latex dust.Because the choosing of this method is low to rate, on this basis, proposed simulation and produced the test tapping method that goes up rubber tapping, promptly 2-3 is set rubber tapping continuously age, measure latex output.The test tapping method does not have the advantage of " early " and " province ", but than other Forecasting Methodology all " standard ", and all the more accurate along with the increase of the prediction age of tree, this is obviously increasing relevant with the shared proportion of secondary latex dust.At present, because the improvement of breed improvement and culture technique, the age of tree that begins to tap rubber in the natural rubber production is the 5-6 after the field planting ahead of time, so, if postpone predicted time simply, just lost the value of early prediction.Detect the shortcoming that other factor relevant with output all has not " standard " seedling stage, and the latex dust of indication is actually nascent latex dust.
Latex dust is the tissue of the synthetic and storing natural rubber of bamboo grows.Producing closely-related latex dust with natural rubber is secondary latex dust in the trunk bark.Admitted facts are into the quantity and the natural rubber output significant correlation of the secondary latex dust row in the rubbery trunk bark in age, be other factor incomparable (Gomez J.B.Anatomy of Hevea and its influence on latex production.Kuala Lumpur:Malaysia Rubber Research and Development Board, 1982.).The ability that how much depends on vascular cambium differentiation latex dust of secondary latex dust number of columns.But, because stage and organ specificity that secondary latex dust is grown, in the newborn coppice shoot of bamboo grows, there is not secondary latex dust, and the difference of secondary latex dust column number on 2-3 treelet in age, can't show (Zhou Zhong gives birth. external Para rubber tree output method for early prediction research overview. the tropical agriculture science, 1993, the 2 phases, 77-83), so, never find a kind of method of predicting secondary latex dust differentiation capability in seedling stage.
Summary of the invention
The purpose of this invention is to provide the method for predicting differentiation ability of rubber tree secondary milk tube a kind of seedling stage.
The technical solution adopted for the present invention to solve the technical problems is:
A) Mei Nian 10-11 month, the bamboo grows germplasm saw bar with preserving in the nursery makes stub germinating branch, and promptly coppice shoot is a material with the newborn coppice shoot with 3-5 elongation unit; B) the mechanical wounding position is that the 1st of coppice shoot extends the stem that unit living scaly leaf; C) be 3-7 month the season of mechanical wounding processing, and it is fine handling the same day; D) phenology of coppice shoot is the blade maturation of the 1st elongation unit, and terminal bud is inactive.
Described mechanical wounding method is, uses the single or double blade to scrape off epidermis and part cortex, and the surface of scratch is directly exposed in the air 2-10 hour.
The invention has the beneficial effects as follows: the secondary latex dust differentiation capability of predicting Amazon wild germplasm and Brunswick Chinese germplasm well in seedling stage.
Embodiment
The present invention is further described below in conjunction with embodiment.
1, material: in annual October, the bamboo grows germplasm saw bar with preserving in the nursery makes stub germinating branch, and promptly coppice shoot is a material with the newborn coppice shoot with 3 elongation units;
2, handle season: annual May;
3, into treatment sites: the 1st elongation unit of coppice shoot the stem of giving birth to scaly leaf;
4, the coppice shoot phenophase: the blade maturation of the 1st elongation unit, terminal bud is inactive;
5, weather condition: fine day.
Described mechanical wounding method is, uses the single or double blade to scrape off epidermis and part cortex, and the surface of scratch is directly exposed in the air 6 hours.
Claims (2)
1. method of predicting differentiation ability of rubber tree secondary milk tube seedling stage is characterized in that:
A) Mei Nian 10-11 month, the bamboo grows germplasm saw bar with preserving in the nursery makes stub germinating branch, and promptly coppice shoot is a material with the newborn coppice shoot with 3-5 elongation unit; B) the mechanical wounding position is that the 1st of coppice shoot extends the stem that unit living scaly leaf; C) be 3-7 month the season of mechanical wounding processing, and it is fine handling the same day; D) phenology of coppice shoot is the blade maturation of the 1st elongation unit, and terminal bud is inactive.
2. predict the method for differentiation ability of rubber tree secondary milk tube a kind of seedling stage according to claim 1, it is characterized in that: described mechanical wounding method is, use the single or double blade to scrape off epidermis and part cortex, the surface of scratch is directly exposed in the air 2-10 hour.
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| CN2008100033527A CN101223855B (en) | 2008-01-14 | 2008-01-14 | Method for predicting differentiation ability of rubber tree secondary milk tube in seedling stage |
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| CN2008100033527A CN101223855B (en) | 2008-01-14 | 2008-01-14 | Method for predicting differentiation ability of rubber tree secondary milk tube in seedling stage |
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| CN101223855B CN101223855B (en) | 2011-05-18 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103798069A (en) * | 2014-02-13 | 2014-05-21 | 中国热带农业科学院橡胶研究所 | Method for screening rubber tree yield conditioning agents |
| CN105160430A (en) * | 2015-08-26 | 2015-12-16 | 中国热带农业科学院橡胶研究所 | Prediction method for superior plants in seedling stage of rubber trees |
| CN105928759A (en) * | 2016-04-28 | 2016-09-07 | 江苏农林职业技术学院 | Manufacturing method of epidermis for Tillandsia epidermis structure observation |
| CN110432141A (en) * | 2019-08-29 | 2019-11-12 | 中国热带农业科学院橡胶研究所 | A kind of method of the late-maturing high yield rubber tree germplasm of Seedling selection |
| CN113846177A (en) * | 2021-07-30 | 2021-12-28 | 中国热带农业科学院橡胶研究所 | SNP molecular marker for rubber tree secondary emulsion tube array number and application thereof |
-
2008
- 2008-01-14 CN CN2008100033527A patent/CN101223855B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103798069A (en) * | 2014-02-13 | 2014-05-21 | 中国热带农业科学院橡胶研究所 | Method for screening rubber tree yield conditioning agents |
| CN103798069B (en) * | 2014-02-13 | 2015-05-13 | 中国热带农业科学院橡胶研究所 | Method for screening rubber tree yield conditioning agents |
| CN105160430A (en) * | 2015-08-26 | 2015-12-16 | 中国热带农业科学院橡胶研究所 | Prediction method for superior plants in seedling stage of rubber trees |
| CN105928759A (en) * | 2016-04-28 | 2016-09-07 | 江苏农林职业技术学院 | Manufacturing method of epidermis for Tillandsia epidermis structure observation |
| CN110432141A (en) * | 2019-08-29 | 2019-11-12 | 中国热带农业科学院橡胶研究所 | A kind of method of the late-maturing high yield rubber tree germplasm of Seedling selection |
| CN113846177A (en) * | 2021-07-30 | 2021-12-28 | 中国热带农业科学院橡胶研究所 | SNP molecular marker for rubber tree secondary emulsion tube array number and application thereof |
| CN113846177B (en) * | 2021-07-30 | 2023-04-25 | 中国热带农业科学院橡胶研究所 | SNP molecular marker for number of secondary emulsion tubes of rubber tree and application of SNP molecular marker |
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| CN101223855B (en) | 2011-05-18 |
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