CN101216489A - Test paper for rapidly detecting hog cholera antibody and method for making same - Google Patents
Test paper for rapidly detecting hog cholera antibody and method for making same Download PDFInfo
- Publication number
- CN101216489A CN101216489A CNA2008100004492A CN200810000449A CN101216489A CN 101216489 A CN101216489 A CN 101216489A CN A2008100004492 A CNA2008100004492 A CN A2008100004492A CN 200810000449 A CN200810000449 A CN 200810000449A CN 101216489 A CN101216489 A CN 101216489A
- Authority
- CN
- China
- Prior art keywords
- swine fever
- membrane
- test strips
- glass fibre
- nitrocellulose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a test paper for rapidly detecting swine fever antibody and a preparation method thereof. The inventive test paper consists of a sample pad (4), a glass fiber member (3), a cellulose nitrate membrane (2), a water absorbent pad (1) and a support (5); wherein the cellulose nitrate membrane contains a detection line (6) coated by swine fever virus E2 protein and a reference line (7) coated with rabbit anti swine fever antibody; and the glass fiber member is combined with swine fever virus E2 protein marked by colloidal gold. The test paper can rapidly detect possibly present swine fever antibody in a sample to achieve rapid detection and timely epidemic control, thus creating favorable conditions for further separation and identification. The test paper has the advantages of convenient, rapid and easy usage, clear result, easy generalization, and no need for special instrument and equipment as well as professionals, and is suitable for large-batch onsite detection for base layer and for sudden events. The test paper is suitable for epidemic inquisition and performs assistant effect on swine fever virus infection diagnosis.
Description
Technical field
The present invention relates to a kind of test strips that detects Animal diseases, relate in particular to a kind of test strips of fast detecting hog cholera antibody, the invention still further relates to preparation method's and methods for using them of this test strips, belong to the Animal diseases inspection and quarantine field.
Background technology
Swine fever is a kind of acute, hot, contact, the deadly infectious disease that is caused by CSFV, no matter the live pig of any kind, age in days all can be taken place, does not also have seasonality; This disease is in case take place, and the case fatality rate height loses hugely, and OIE (OIE) classifies it as category-A infectious disease, and it is that a class zoonosis manages that China receives it, and the anti-system work of this disease is attached great importance to.
At present, known hog cholera antibody quick detection kit (ELISA method) is the method that adopts hog cholera antibody in the Enzyme-multiplied immune technique test sample (serum).That is: adopt the weak poison of swine fever deactivation or attenuated vaccine strain is purified, concentrate the antigen coated microwell plate of making, in test, the control serum and the serum to be checked that add dilution, behind incubation, if contain the swine fever specific antibody in the sample, then will combine, after unconjugated antibody and other compositions are removed in washing with antigen on the microwell plate; Add ELIAS secondary antibody again, combine with antigen antibody complex generation specificity on the microwell plate; Remove unconjugated enzyme conjugates through washing again, in the hole, add TMB or OPD substrate solution, form coloured product with enzyme reaction, add the stop buffer reaction after, measure OD value in each reacting hole with microplate reader fixed wave length (450nm or 630nm).ELISA is fit to the detection of gross sample, becomes a kind of detection method of routine.But the preparation process of this method antigen must be through loaded down with trivial details patterns such as viral cellular incubation, viral purifications, and specificity is not high, poor stability, and the cost height; Need special instrument and equipment such as microplate reader to be used during detection, the detecting operation personnel need pass through professional training; Operating process is relatively complicated; It is long to detect the required time; Detect required expense height.
Summary of the invention
Technical matters to be solved by this invention is to overcome the problem that prior art exists, a kind of test strips that can the fast detecting hog cholera antibody is provided, this test strips does not need the technical professional to operate, method is simple and convenient and easy to study, need not carry out auxiliary detection by any instrument, testing result specificity height, good reproducibility.
Technical matters to be solved by this invention is achieved through the following technical solutions:
A kind of test strips of fast detecting hog cholera antibody is made up of sample pad, glass fibre membrane, nitrocellulose filter (NCM), adsorptive pads and holder; Described nitrocellulose filter contains a detection line that is formed by swine fever E2 albumen bag and the control line that is formed by the anti-hog cholera antibody bag of rabbit; Described glass fibre membrane is combined with the swine fever E2 albumen of colloid gold label; Glass fibre membrane, nitrocellulose membrane and adsorptive pads are connected and in the following sequence attached on the holder: glass fibre membrane is connected with a end near the nitrocellulose membrane detection line, and adsorptive pads is connected with an end of close nitrocellulose membrane control line; Sample pad is attached on the glass fibre membrane, and an end of sample pad is connected mutually with nitrocellulose filter.
Wherein, as long as described holder has certain rigidity, with sample pad, glass fibre membrane, nitrocellulose filter and adsorptive pads load thereon, the purpose that reaches support and load promptly can be used as holder of the present invention, can select for use various materials as holder of the present invention, for example plastic plate (being preferably PVC), cardboard, aluminium sheet etc.
Described swine fever E2 albumen can be prepared according to the gene engineering method of routine or express, and these methods all are that those skilled in the art can grasp or understand thoroughly.
The anti-hog cholera antibody of described rabbit can prepare with reference to following method: adopt 3 of the negative rabbit of multi-point injection method immunity with the weak poison of swine fever, every 2 all booster immunizations 1 time, carry out last immunity blood sampling after 10 days altogether 3 times, separation of serum gets the anti-swine fever IgG of rabbit behind the purifying.
Detection line on the described nitrocellulose filter (this nitrocellulose filter also can be replaced by nylon membrane) and the interval between the control line are preferably 3-5mm, more preferably 4mm.
A kind of method for preparing the test strips of detection hog cholera antibody of the present invention comprises:
(1) be sprayed on the glass fibre membrane with the swine fever E2 albumen composition of Membrane jetter colloid gold label, standby;
(2) with swine fever E2 albumen and the anti-hog cholera antibody IgG of rabbit successively at interval 3-5mm be sprayed on the nitrocellulose membrane, respectively as detection line and control line, will bag by good all the other protein binding sites of cellulose nitrate membrane closure, washing, drying, standby;
(3) stick on nitrocellulose membrane, glass fibre membrane, sample pad, adsorptive pads on the support plate in the following sequence: glass fibre membrane is connected a end near the nitrocellulose membrane detection line, and the edge is attached on the nitrocellulose filter; Sample pad is connected with nitrocellulose filter attached on the glass fibre membrane; The absorbent filter plate is connected the other end of nitrocellulose filter, and the edge is attached on the nitrocellulose filter;
(4) the support plate material that glues is cut into the test strips that 60mm is long, 4mm is wide, promptly.
Test strips of the present invention detects the method for hog cholera antibody:
1 method of operating: before detection, earlier sample and test strips are placed under the room temperature condition and place a period of time (10 minutes), make it restore to room temperature; From aluminium foil bag, take out test strip; In oval well, add 3-5 and drip (100-200 μ l) pig blood to be checked or blood serum sample; Test strips is kept flat on the table, at room temperature leave standstill 20 minutes result of determination;
2 results judge:
Invalid: as colo(u)r streak not occur at control line and detection line;
Negative: as a colo(u)r streak to occur at control line, colo(u)r streak do not occur at detection line;
Weak positive: as the purplish red colo(u)r streak that color is darker to occur at control line, and occur the purplish red colo(u)r streak of a very slight color at detection line;
Positive: a purplish red colo(u)r streak that color is darker respectively occurs at control line and detection line, the antibody expression level in the sample is high more, and detection line colo(u)r streak color is dark more.
The present invention utilizes technique for gene engineering to express swine fever E2 albumen, adopts enzyme linked immunological principle and rete to analyse the test strips that technology is made the hog cholera antibody in fast detecting pig blood or the serum.Compare with IFA with ELISA, test strips of the present invention has following remarkable advantages: security is good, need not to cultivate virus itself, has avoided because of operating the virus diffusion that virus causes; Can prepare in enormous quantities, technology is simple, low production cost; The antigenic component stable uniform, easy and simple to handle laborsaving, without instrument, testing result specificity height, good reproducibility.Whole experiment only needs 15 minutes.Easy and simple to handle, quick, accurate, highly sensitive, directly perceived, result judges easily.
Association colloid gold labelling technique of the present invention and rete are analysed technology, but the hog cholera antibody that may exist in the fast detecting sample, reach fast detecting, in time control the purpose of epidemic situation, for next step isolation identification has been created advantage, save lot of manpower and material resources, easily and fast, easy, do not needed special instruments and equipment, do not need professional training, the result is clear easily to be distinguished; Mass field detection simple to operate, as to be easy to promote, be fit to basic unit and accident is fit to epidemiological survey, and booster action is played in diagnosis to swine fever virus infection.
Description of drawings
The front schematic view of Fig. 1 test strips of the present invention;
The side schematic view of Fig. 2 test strips of the present invention;
Fig. 3 testing result synoptic diagram: be followed successively by from left to right: detection line and control line colour developing are positive; The control line colour developing is negative; Detection line and control line two bands do not develop the color for invalid.
Description of reference numerals: 1-adsorptive pads; (6: bag is by the swine fever E2 albumen of gene engineering expression for the 2-nitrocellulose membrane; 7: bag is by the anti-hog cholera antibody IgG of rabbit); 3-contains the glass fibre membrane of colloid gold label antigen; The 4-sample pad; 5-reacts holder.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall within the scope of protection of the present invention the details of technical solution of the present invention and form.
Embodiment 1 the present invention detects the preparation of hog cholera antibody test strips
The preparation of 1 swine fever E2 albumen
1. material source
(1) viral source.The weak poison of swine fever (Harbin Veterinary Medicine Inst., China Academy of Agriculture's preparation).
(2) carrier, recipient bacterium and reagent source.Recipient bacterium BL21: Harbin Veterinary Medicine Inst., China Academy of Agriculture's diagnosis and epidemiology center; PET-30a, restriction enzyme and various modification enzyme are purchased the precious biotech firm in Dalian; IPTG, kanamycins: the worker is given birth in Shanghai.
(3) primer is synthetic.Amplification contains the used primer of genetic fragment of coding B/C antigenic region: P upstream: (5 ' tcgaattcatgcgtctagcctgca 3 '); The P downstream: (5 ' tggtgagtgagtaaagcccccttat 3 '), upstream and downstream primer comprise respectively and are designed to EcoRI, PstI restriction enzyme site, the 1st amino acids to the 76 amino acids of amplification raq gene.
(4) reagent source.Taq archaeal dna polymerase, T
4Dna ligase and various restriction endonuclease: Takara company; Other reagent is homemade.
2. swine fever E2 induction expression of protein
(1) extraction of viral RNA and Gene RT-PCR amplification: the extraction of RNA is undertaken by Trizol kit instructions; According to CSFV design a pair of Auele Specific Primer at the B/C gene (on seeing) among the GeneBank, reverse transcription is undertaken by reverse transcription kit instructions.Get 5 μ l cDNA products as the PCR reaction.
(2) structure of expression vector
After the recovery of PCR product glue, be connected with the PMD18-T carrier, obtain positive recombinant plasmid after the conversion, called after TB/C.Utilize the restriction enzyme site of upstream primer and the restriction enzyme site on the carrier, the prokaryotic expression carrier PET30a that reclaims B/C gene and same processing behind the double digestion carries out T
4Dna ligase connects, and transforms the competence of BL21 strain preparation.Extract plasmid in a small amount.
(3) abduction delivering of PCR positive strain
The BL21 bacterial classification that will contain recombinant plasmid PETB/C is rule containing on the LB flat board of kanamycins, 37 ℃ of overnight incubation, the single bacterium colony of picking, when 37 ℃ of shaking tables are cultured to OD600 and are 0.6-0.7 in containing that antibiotic LB of card, add derivant IPTG to final concentration 1mM, continue jolting and cultivated 3-6 hour, the results bacterium.After centrifugal thalline is washed twice with 0.5M NaCl, 20mM TrisCl (pH=7.6), use the PBS suspension cell then.
(4) purifying of swine fever E2 albumen
Thalline behind the ultrasonic degradation is centrifugal with 10000r/min, behind the 0.22um filtering with microporous membrane, add 1mL nickel affinity chromatography substrate, dress post, 4 ℃ of effect 30min.Successively with 10～20 times of bed volumes, contain 10mM imidazoles and 20mM imidazoles, the phosphate buffer of 500mM NaCl washing 3 times; Contain the reorganization E2 albumen of His2tag again with the phosphate buffer wash-out that contains 100～500mM imidazoles, be in charge of collection, elution flow rate is controlled at 2～3mL/min.
The anti-swine fever hyper-immune serum of 2 preparation rabbits
Adopt 3 of the negative rabbit of multi-point injection method immunity with poison (Harbin Veterinary Medicine Inst., China Academy of Agriculture's productions) a little less than the swine fever,, carry out altogether 3 times every 2 all booster immunizations 1 time, last immunity blood sampling after 10 days, separation of serum gets the anti-swine fever IgG of rabbit behind the purifying.
The preparation of 3 colloid gold particles
(Shanghai chemical reagents corporation of Chinese Medicine group) is mixed with 0.01% aqueous solution with chlorauride, after getting 100ml and boiling 2min, adds trisodium citrate (1%) 2ml while stirring, boil become claret to solution colour after, continue to boil to suitable concentration (OD
535=0.9312), the cooling back adds DDW and returns to original volume, puts 4 ℃ of preservations.
4 colloid gold label E2 protein Preparation and purifying
With E2 albumen doubling dilution to be marked, get 100 μ l respectively and add in the 1ml collaurum, add 10%NaCl 100 μ l, 4 ℃ of static 1h behind the 10min.The highly diluted multiple of getting that the collaurum color do not change is as the criterion, and adds 30% on this basis and is the optimum mark amount.Get a certain amount of deployed collaurum 0.2mol/L K
2CO
3Transfer to pH=9.0, add antigen expressed E2 albumen by the optimum mark amount, room temperature effect 20min, (making final concentration is to use behind 1%, 4 ℃ of placement 2h for pH8.0,20mmol/L) Pei Zhi BSA to add Tris-HCl.Gold is marked the centrifugal 30min of antigen 3000r/min, get supernatant, the centrifugal 60min of 60000g, precipitation returns to original volume with the PBS dissolving that 0.02mol/L pH7.2 contains 0.1%BSA.Super again from 1 time, precipitation makes OD with a little above-mentioned PBS dissolving
535nm=1.5.0.22 μ m membrane filtration, 4 ℃ of preservations are standby.
5, the assembling of test strips
(1) be sprayed on the glass fibre membrane with the swine fever E2 albumen composition of Membrane jetter colloid gold label, standby;
(2) with swine fever E2 albumen and the anti-swine fever IgG of rabbit successively at interval 3-5mm be sprayed on the nitrocellulose membrane, respectively as detection line and control line, will bag by good all the other protein binding sites of cellulose nitrate membrane closure, washing, drying, standby;
(3) be bonded at nitrocellulose membrane, glass fibre membrane, sample pad, adsorptive pads on the support plate in the following sequence: glass fibre membrane is connected a end near the nitrocellulose membrane detection line, and the edge is attached on the nitrocellulose filter; Sample pad is connected with nitrocellulose filter attached on the glass fibre membrane; The absorbent filter plate is connected the other end of nitrocellulose filter, and the edge is attached on the nitrocellulose filter;
(4) the support plate material that glues is cut into the test strips that 60mm is long, 4mm is wide, promptly.
The correlation test of test example 1 hog cholera antibody quick detection test paper bar of the present invention
1 material source
CSFV antigen: Harbin Veterinary Medicine Inst., China Academy of Agriculture provides;
The preparation of swine fever hyper-immune serum: the attenuated vaccine of producing with Harbin Veterinary Medicine Inst., China Academy of Agriculture, with 4 of the negative health pig of multi-point injection method immunity, every 2 all booster immunizations 1 time, carry out last 1 immunity blood sampling 10 day after tomorrow 3 times altogether; Separation of serum.
Reference serum: pig circular ring virus (PCV) positive serum, pig flow pattern diarrhea virus (PEDV) positive serum, transmissible gastro-enteritis virus (TGEV) positive serum, porcine rotavirus (PRoV) positive serum, PRV (PRV) positive serum, pig parvoviral (PPV) positive serum.
Serum to be checked: pick up from 1285 part serum on 36 pig farms, all parts of the country such as Heilungkiang, Tianjin, Fujian and whole blood (wherein 460 adopted pig whole blood for the hypothesis health pig), respectively 15 parts of chicken serum, duck serum, goose serum, sheep blood serums amount to 825 parts of serum and 460 parts of whole bloods.And number above-mentioned serum, whole blood to be checked;
The contrast agents box: swine fever ELISA kit is provided by Harbin Veterinary Medicine Inst., China Academy of Agriculture; Blood clotting method kit is provided by Lanzhou Veterinary Inst., Chinese Acedemy of Agaricultural Sciences.
One, test strips sensitivity tests of the present invention
With the positive hyper-immune serum of a swine fever, use test strips of the present invention (embodiment 1 is prepared) and indirect ELISA reagent kit to detect simultaneously;
Test findings: limit of identification, ELISA kit are 1: 1920-3840, test strips of the present invention (GICA) is 1: 1920.Sensitivity similar.
Respectively above-mentioned 1285 parts of serum are detected with test strips of the present invention, hog cholera antibody ELISA kit and hog cholera indirect hemagglutination method antigenic reagent box, as a result basically identical.Wherein, in 825 parts of porcine blood serum, positive 738 parts, negative 147 parts.The coincidence rate of result who detects with test strips of the present invention and ELISA, two kinds of method testing results of indirect hemagglutination method is respectively 99.86% and 98.92%.
Two, test strips specificity of the present invention and coincidence rate test
With test strips of the present invention pig circular ring virus (PCV) positive serum, pig flow pattern diarrhea virus (PEDV) positive serum, transmissible gastro-enteritis virus (TGEV) positive serum, porcine rotavirus (PRoV) positive serum, PRV (PRV) positive serum, pig parvoviral (PPV) positive serum are detected, punctation does not all appear in testing result, punctation then occurs with positive hyper-immune serum of the CSFV of purifying and unpurified CSFV positive serum.Respectively above-mentioned 1285 parts of serum are detected with test strips of the present invention, hog cholera antibody ELISA kit and hog cholera indirect hemagglutination method antigenic reagent box, basically identical as a result, wherein, and in 885 parts of porcine blood serum positive 738 parts, negative 147 parts; And chicken, duck, goose, rabbit, sheep blood serum totally 75 parts all negative.When making above-mentioned pig indirect hemagglutination test, in 885 parts of porcine blood serum, the blood clotting titre is in 616 parts in the sample more than 1: 16, and the blood clotting titre is in 122 parts in the sample below 1: 16.75 parts of chickens, duck, goose, sheep blood serums fail to detect.
Three, criticize in and batch between replica test
(1), replica test in (1) batch: 3 parts of negative and positive blood serum samples every duplicate samples in same batch of test strips test is parallel establishes 6 repetitions; (2) criticize between replica test: at 6 batches of products of 5 different tests day replications;
(2) test findings: repeating the coefficient of variation in batch is between the 3.1-8.6%; Repeating the coefficient of variation between batch is between the 4.2-10.3%.
Four, storage life test
Since in January, 2003 the paper slip kit of preserving has been tested, the result shows that the kit storage life is all more than 2 years.The suggestion kit is at-20 ℃, and the term of validity is 12-18 month.
Claims (7)
1. the test strips of a fast detecting hog cholera antibody is characterized in that: comprise sample pad (4), glass fibre membrane (3), nitrocellulose filter (2), adsorptive pads (1) and holder (5); Described nitrocellulose filter contains a detection line (6) that is formed by swine fever E2 albumen bag and the control line (7) that is formed by the anti-hog cholera antibody bag of rabbit; Described glass fibre membrane is combined with the swine fever E2 albumen of colloid gold label; Glass fibre membrane, nitrocellulose membrane and adsorptive pads are connected and in the following sequence attached on the holder: glass fibre membrane is connected with a end near nitrocellulose membrane detection line (6), and adsorptive pads is connected with an end of close nitrocellulose membrane control line (7); Sample pad is attached on the glass fibre membrane, and an end of sample pad is connected mutually with nitrocellulose filter.
2. according to the described test strips of claim 1, it is characterized in that: described holder (5) is plastic plate, cardboard or aluminium sheet.
3. according to the described test strips of claim 1, it is characterized in that: the anti-hog cholera antibody of described rabbit prepares in accordance with the following methods: adopt 3 of the negative rabbit of multi-point injection method immunity with the weak poison of swine fever, every 2 all booster immunizations 1 time, carry out altogether 3 times, last immunity blood sampling after 10 days, separation of serum gets the anti-swine fever IgG of rabbit behind the purifying.
4. according to the test strips of claim 1, it is characterized in that: described nitrocellulose filter can be replaced by nylon membrane.
5. according to the test strips of claim 1, it is characterized in that: be spaced apart 3-5mm between detection line on the described nitrocellulose filter and the control line.
6. according to the test strips of claim 5, it is characterized in that: be spaced apart 4mm between detection line on the described nitrocellulose filter and the control line.
7. method for preparing the test strips of the described detection hog cholera antibody of claim 1 comprises:
(1) be sprayed on the glass fibre membrane with the swine fever E2 albumen composition of Membrane jetter colloid gold label, standby;
(2) with swine fever E2 albumen and the anti-hog cholera antibody IgG of rabbit successively at interval 3-5mm be sprayed on the nitrocellulose membrane, respectively as detection line and control line, will bag by good all the other protein binding sites of cellulose nitrate membrane closure, washing, drying, standby;
(3) be bonded at nitrocellulose membrane, glass fibre membrane, sample pad, adsorptive pads on the support plate in the following sequence: glass fibre membrane is connected a end near the nitrocellulose membrane detection line, and the edge is attached on the nitrocellulose filter; Sample pad is connected with nitrocellulose filter attached on the glass fibre membrane; The absorbent filter plate is connected the other end of nitrocellulose filter, and the edge is attached on the nitrocellulose filter;
(4) the support plate material that glues is cut into the test strips that 60mm is long, 4mm is wide, promptly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100004492A CN101216489A (en) | 2008-01-10 | 2008-01-10 | Test paper for rapidly detecting hog cholera antibody and method for making same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100004492A CN101216489A (en) | 2008-01-10 | 2008-01-10 | Test paper for rapidly detecting hog cholera antibody and method for making same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101216489A true CN101216489A (en) | 2008-07-09 |
Family
ID=39622967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100004492A Pending CN101216489A (en) | 2008-01-10 | 2008-01-10 | Test paper for rapidly detecting hog cholera antibody and method for making same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101216489A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102532281A (en) * | 2012-01-17 | 2012-07-04 | 江苏省农业科学院 | Classical swine fever virus recombinant E2 protein and IgM (immune globulin M) antibody ELISA (enzyme-linked immunosorbent assay) test kit thereof |
| CN105548535A (en) * | 2016-01-21 | 2016-05-04 | 成都微瑞生物科技有限公司 | Classical swine fever antibody detection card and preparation method thereof |
| CN105572381A (en) * | 2015-03-25 | 2016-05-11 | 江苏艾维迪生物科技有限公司 | Preparation of classical swine fever virus antigen colloidal gold test strip |
| CN105974116A (en) * | 2016-07-12 | 2016-09-28 | 郑州中道生物技术有限公司 | Multi-linked rapid detection test strip for pig diseases and preparation method thereof |
| CN108061800A (en) * | 2017-12-08 | 2018-05-22 | 重庆市畜牧科学院 | Hog cholera antibody colloidal-gold detecting-card and preparation method thereof |
| CN109187968A (en) * | 2018-09-19 | 2019-01-11 | 郑州大学 | A kind of swine fever virus and the bigeminy gold mark detection test paper of porcine pseudorabies virus and preparation method thereof |
| CN116359498A (en) * | 2023-02-23 | 2023-06-30 | 兰州兽研生物科技有限公司 | Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1403814A (en) * | 2001-12-15 | 2003-03-19 | 河南省农业科学院生物技术研究所 | Fast clenbuterol hydrochloride detecting test paper strip |
-
2008
- 2008-01-10 CN CNA2008100004492A patent/CN101216489A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1403814A (en) * | 2001-12-15 | 2003-03-19 | 河南省农业科学院生物技术研究所 | Fast clenbuterol hydrochloride detecting test paper strip |
Non-Patent Citations (3)
| Title |
|---|
| 周铁忠 等: "应用猪瘟抗体免疫金标试纸检测", 《现代畜牧兽医》 * |
| 张永国 等: "猪瘟病毒E2基因抗原结构域A、B、C、D区在大肠杆菌中的表达", 《畜牧兽医学报》 * |
| 徐学清 等: "猪瘟病毒E2蛋白B/C抗原区基因在毕赤酵母中的表达与鉴定", 《中国生物工程杂志》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102532281A (en) * | 2012-01-17 | 2012-07-04 | 江苏省农业科学院 | Classical swine fever virus recombinant E2 protein and IgM (immune globulin M) antibody ELISA (enzyme-linked immunosorbent assay) test kit thereof |
| CN102532281B (en) * | 2012-01-17 | 2014-03-05 | 江苏省农业科学院 | Classical swine fever virus recombinant E2 protein and IgM (immune globulin M) antibody ELISA (enzyme-linked immunosorbent assay) test kit thereof |
| CN105572381A (en) * | 2015-03-25 | 2016-05-11 | 江苏艾维迪生物科技有限公司 | Preparation of classical swine fever virus antigen colloidal gold test strip |
| CN105548535A (en) * | 2016-01-21 | 2016-05-04 | 成都微瑞生物科技有限公司 | Classical swine fever antibody detection card and preparation method thereof |
| CN105974116A (en) * | 2016-07-12 | 2016-09-28 | 郑州中道生物技术有限公司 | Multi-linked rapid detection test strip for pig diseases and preparation method thereof |
| CN108061800A (en) * | 2017-12-08 | 2018-05-22 | 重庆市畜牧科学院 | Hog cholera antibody colloidal-gold detecting-card and preparation method thereof |
| CN109187968A (en) * | 2018-09-19 | 2019-01-11 | 郑州大学 | A kind of swine fever virus and the bigeminy gold mark detection test paper of porcine pseudorabies virus and preparation method thereof |
| CN109187968B (en) * | 2018-09-19 | 2022-01-28 | 郑州大学 | Bivalent gold-labeled test paper for detecting classical swine fever virus and porcine pseudorabies virus and preparation method thereof |
| CN116359498A (en) * | 2023-02-23 | 2023-06-30 | 兰州兽研生物科技有限公司 | Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies |
| CN116359498B (en) * | 2023-02-23 | 2024-06-11 | 兰州兽研生物科技有限公司 | Immune chromatography test paper card for joint inspection of foot-and-mouth disease, african swine fever, swine fever and porcine reproductive and respiratory syndrome virus antibodies |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101216489A (en) | Test paper for rapidly detecting hog cholera antibody and method for making same | |
| CN101216488A (en) | Test paper for detecting pig breeding and respiration syndrome antibody and method for making same | |
| CN101216492A (en) | Test paper for rapidly detecting pseudorabies antibody and method for making same | |
| CN101762705A (en) | Colloidal gold immunochromatographic test strip for detecting wild-type classical swine fever virus | |
| CN103360472B (en) | Acian Metapneumovirus antibody ELISA detection kit | |
| CN105753947A (en) | Infectious bovine rhinotracheitis virus gD protein and application thereof | |
| CN101363858B (en) | Test paper strip for detecting PRRSV antibody colloidal gold, method for making same and applications | |
| CN102174109A (en) | Acquired immune deficiency syndrome virus and treponema pallidum fusion protein as well as preparation method and application thereof | |
| CN105277693A (en) | Human parainfluenza virus quantum dot immunochromatography typing detection card, preparation method and applications | |
| CN110257276A (en) | A kind of inert carrier Escherichia coli and its potential application | |
| CN106636004A (en) | TMV-CMV-PVY three-virus (tobacco mosaic virus, cucumber mosaic virus and potato virus) colloidal gold rapid test strip | |
| CN103235119A (en) | A diagnosis antigen for Toxoplasma gondii infection and a preparation method and applications thereof | |
| CN103995136B (en) | Rapid colloidal gold detection test strip for pig porcine reproductive and respiratory syndrome virus | |
| CN101363856B (en) | Test paper strip for rapidly detecting morbilli and rubella virus IgG antibody colloidal gold | |
| CN108333369B (en) | A kind of porcine reproductive and respiratory syndrome virus antibody detection method and its dedicated test card | |
| CN101706499A (en) | FLAG fusion tag colloidal gold test strip and preparation method thereof | |
| CN105838682A (en) | Specific monoclonal antibody cell strain for bovine viral diarrhea virus and applications of specific monoclonal antibody cell strain | |
| CN104569425A (en) | Antigen protein specifically bound with tyrosine phosphatase antibody | |
| CN111505289A (en) | Peste des petits ruminants detection kit | |
| CN101833003A (en) | Vesicular stomatitis virus antibody colloidal goldtest paper tape and preparation method thereof | |
| CN101363857B (en) | Colloidal gold test paper strip for detecting morbilli and rubella virus IgM antibody, method for making same and applications | |
| CN101363865A (en) | Test paper strip for detecting PRRSV antibody colloidal gold, method for making same and applications | |
| CN108957002A (en) | Antibody against swine fever virus quantitative testing test paper card with double antigens sandwich and double detection lines | |
| Vesikari | Immune response in rubella infection | |
| CN103983780A (en) | Colloidal gold immunochromatograohic assay test strip for detecting pigeon I-type paramyxovirus and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20080709 |