CN101213980A - 用补骨脂定防治植物真菌病害的生物农药 - Google Patents
用补骨脂定防治植物真菌病害的生物农药 Download PDFInfo
- Publication number
- CN101213980A CN101213980A CNA2008100100165A CN200810010016A CN101213980A CN 101213980 A CN101213980 A CN 101213980A CN A2008100100165 A CNA2008100100165 A CN A2008100100165A CN 200810010016 A CN200810010016 A CN 200810010016A CN 101213980 A CN101213980 A CN 101213980A
- Authority
- CN
- China
- Prior art keywords
- ethyl acetate
- psoralidin
- control plant
- psoralidine
- biological pesticide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229930030856 Psoralidin Natural products 0.000 title claims abstract description 17
- 208000031888 Mycoses Diseases 0.000 title claims description 7
- 239000000575 pesticide Substances 0.000 title abstract description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 18
- 241000196324 Embryophyta Species 0.000 claims abstract description 16
- 239000000284 extract Substances 0.000 claims abstract description 6
- 238000003810 ethyl acetate extraction Methods 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 150000001875 compounds Chemical class 0.000 claims abstract description 4
- 239000000469 ethanolic extract Substances 0.000 claims abstract description 4
- 238000010828 elution Methods 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 3
- 230000000853 biopesticidal effect Effects 0.000 claims description 7
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 6
- HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 claims description 4
- 244000226566 Psoralea corylifolia Species 0.000 claims description 2
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 claims description 2
- 230000002829 reductive effect Effects 0.000 claims description 2
- 238000010898 silica gel chromatography Methods 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 240000008067 Cucumis sativus Species 0.000 abstract description 12
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 abstract description 12
- 201000010099 disease Diseases 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 7
- 241000223218 Fusarium Species 0.000 abstract description 6
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 abstract description 5
- 244000052769 pathogen Species 0.000 abstract description 5
- 230000001717 pathogenic effect Effects 0.000 abstract description 5
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 3
- 235000009566 rice Nutrition 0.000 abstract description 3
- 235000021307 Triticum Nutrition 0.000 abstract description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 4
- 239000003208 petroleum Substances 0.000 abstract 3
- 241000233866 Fungi Species 0.000 abstract 1
- 241001547796 Macrophoma Species 0.000 abstract 1
- 241001344131 Magnaporthe grisea Species 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 241000233629 Phytophthora parasitica Species 0.000 abstract 1
- 206010039509 Scab Diseases 0.000 abstract 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 1
- 241000209140 Triticum Species 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- SRCZQMGIVIYBBJ-UHFFFAOYSA-N ethoxyethane;ethyl acetate Chemical compound CCOCC.CCOC(C)=O SRCZQMGIVIYBBJ-UHFFFAOYSA-N 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 244000000003 plant pathogen Species 0.000 abstract 1
- 239000000741 silica gel Substances 0.000 abstract 1
- 229910002027 silica gel Inorganic materials 0.000 abstract 1
- 241001512566 Valsa mali Species 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- 241000220225 Malus Species 0.000 description 7
- 230000002401 inhibitory effect Effects 0.000 description 7
- 241000233616 Phytophthora capsici Species 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 241000223195 Fusarium graminearum Species 0.000 description 5
- 241001330975 Magnaporthe oryzae Species 0.000 description 5
- 241000813090 Rhizoctonia solani Species 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 4
- 239000005842 Thiophanate-methyl Substances 0.000 description 4
- 239000006013 carbendazim Substances 0.000 description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 4
- 239000000287 crude extract Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 4
- 241000209094 Oryza Species 0.000 description 3
- GAMFEMAXLMWCRG-UHFFFAOYSA-N bis(dimethylcarbamothioylsulfanyl)arsanyl n,n-dimethylcarbamodithioate Chemical compound CN(C)C(=S)S[As](SC(=S)N(C)C)SC(=S)N(C)C GAMFEMAXLMWCRG-UHFFFAOYSA-N 0.000 description 3
- 239000000645 desinfectant Substances 0.000 description 3
- 239000000470 constituent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000012055 fruits and vegetables Nutrition 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 241001530056 Athelia rolfsii Species 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000222235 Colletotrichum orbiculare Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241001115351 Physalospora Species 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 150000002611 lead compounds Chemical class 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 235000010603 pastilles Nutrition 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
用补骨脂定防治植物真菌病害的生物农药，本发明涉及一种植物用生物农药，特别是涉及一种用补骨脂定防治植物真菌病害的生物农药。化合物是干燥补骨脂种子5kg，用70％工业乙醇冷浸提取四次，浸出液减压浓缩得乙醇提取物，加水混溶，依次用石油醚、乙酸乙酯萃取；取乙酸乙酯萃取部位浸膏100g，经反复硅胶柱层析，用石油醚－乙酸乙酯梯度洗脱，分离得到补骨脂定(50mg)(石油醚∶乙酸乙酯＝4∶1部分)。抑制常见植物病原菌，如苹果腐烂病菌、苹果轮纹病菌、水稻稻瘟病菌、水稻纹枯病菌、黄瓜枯萎病菌、小麦赤霉病菌、黄瓜炭疽病菌、辣椒疫霉病菌等。
Description
技术领域
本发明涉及一种植物用生物农药，特别是涉及一种用补骨脂定防治植物真菌病害的生物农药。
背景技术
据统计我国水稻种植面积2882万公顷(2001)，小麦种植面积2199万公顷(2003)，苹果面积230万公顷，蔬菜面积逐年增加。苹果腐烂、苹果轮纹、水稻稻瘟、水稻纹枯、黄瓜枯萎、小麦赤霉、黄瓜炭疽、辣椒疫霉是上述作物、果蔬生产中常见的植物病害，对于上述病害的防治主要采用化学农药，比如：50％多菌灵、70％甲基托布津、银泰等。但因一些常规品种的长期使用使病原菌产生抗药性，致使田间用药量逐年加大，给人类的生存环境及健康带来了不可逆转的负面影响。因此，高效、低毒、低残留、与环境友善同时具有自主知识产权新农药品种的创制迫在眉睫。从豆科植物补骨脂中提取的天然有效成分补骨脂定(Psoralidin)对作物、果蔬生产中常见的植物病害，具有优异的防治效果，因此，补骨脂定(Psoralidin)可以开发为具有广阔前景的植物源生物农药，同时，可以作为先导化合物创制新药。
发明内容
本发明的目的在于提供一种用补骨脂定(Psoralidin)抑制常见植物病原菌，如苹果腐烂病菌、苹果轮纹病菌、水稻稻瘟病菌、水稻纹枯病菌、黄瓜枯萎病菌、小麦赤霉病菌、黄瓜炭疽病菌、辣椒疫霉病菌等。
本发明的目的是通过以下技术方案实现的：
用补骨脂定防治植物真菌病害的生物农药，其化合物是干燥补骨脂种子5Kg，用70％工业乙醇冷浸提取四次，浸出液减压浓缩得乙醇提取物，加水混溶，依次用石油醚、乙酸乙酯萃取；取乙酸乙酯萃取部位浸膏100g，经反复硅胶柱层析，用石油醚一乙酸乙酯梯度洗脱，分离得到补骨脂定(50mg)(石油醚∶乙酸乙酯＝4∶1部分)。
所述的用补骨脂定防治植物真菌病害的生物农药，所述的补骨脂定，无色针晶，mp 290～292℃；TLC检查在紫外下为蓝荧光斑点；ESIMS：m/z 337.4，[M+H]+，335.3[M-H]；HNMR(COCD3+CDCl3)：1.83(3H，s，CH3)，1.82(3H，s，CH3)，3.42(2H，d，J＝7.2Hz，H-1′)，5.41(1H，m，H-2′)，6.92(1H，s，H-4)，6.96(1H，dd，J＝1.9，8.4Hz，H-8)，7.13(1H，d，J＝1.8Hz，H-10)，7.68(1H，s，H-1)，7.82(1H，d，J＝8.4Hz，H-7)；
补骨脂定结构式：
本发明的优点与效果是：
补骨脂定(Psoralidin)作为农用杀菌剂具有以下优势：
1.补骨脂定(Psoralidin)是天然有效成分，本身作为农用杀菌剂具有高效、低毒、低残留、不易产生抗药性等特点。
2.药效高。补骨脂定、福美胂、银泰、甲基托布津、多菌灵5种原药对苹果腐烂病菌菌丝生长的抑制作用EC50分别为1.35、8.93、26.18、9.16、1.47mg/L，EC90分别为15.33、128.98、93.37、147.85、26.86mg/L。可见，补骨脂定的抑菌活性高于防治苹果腐烂病菌的其它药剂。
3.杀菌谱广。补骨脂定对苹果腐烂病菌、苹果轮纹病菌、水稻稻瘟病菌、水稻纹枯病菌、黄瓜枯萎病菌、小麦赤霉病菌、黄瓜炭疽病菌、辣椒疫霉病菌等多种常见植物病害均具有很好的抑制活性。
具体实施方式
补骨脂定(Psoralidin)可以作为农用杀菌剂用于防治苹果腐烂病菌(Valsa mali)，苹果轮纹病菌(Physalospora piricola)，水稻稻瘟病菌(Pyricularia oryzae)，水稻纹枯病菌(Rhizoctonia solani Kuhn)，黄瓜枯萎病菌(Fusarium oxysporum)，小麦赤霉病菌(Gibberella zeae)，黄瓜炭疽病菌(Colletotrichum orbiculare)辣椒疫霉病菌(Phytophthoracapsici)等引起的多种植物病害。
实例1：用丙酮或二甲基亚砜将补骨脂定(Psoralidin)溶解配成10g/L的粗提物母液。加入适量粗提物母液于50℃的PDA培养基中，充分混匀，配成粗提物终浓度分别为0.12mg/L、0.6mg/L、3mg/L、15mg/L、75mg/L的含药培养基，以等体积的丙酮或二甲基亚砜加入到PDA培养基中作对照，然后倒入直径90mm培养皿内制成平板，备用。用直径6mm的打孔器从事先在PDA平板上28℃下培养7～12d的各供试病菌菌落边缘处切取菌饼，并移植于上述含不同浓度的粗提物平板和对照平板中央，每次处理重复3次，置28℃下培养7～12d，测量菌落直径。每菌落取相交垂直线方向测其直径，以其均值减去菌饼直径即得菌落净生长直径(CK)，相对抑制率(％)＝(对照净生长量一处理净生长量)/对照净生长量×100。根据病菌抑制率等有关指标，采用相应的数据分析软件，计算出EC50值及EC90值限。
补骨脂定对几种常见植物病原菌菌丝生长的抑制作用
| 抑制作用 | 苹果腐烂病菌 | 苹果轮纹病菌 | 水稻稻瘟病菌 | 水稻纹枯病菌 | 黄瓜炭疽病菌 | 黄瓜枯萎病菌 | 小麦赤霉病菌 | 辣椒疫霉病菌 |
| EC50(mg/L)EC90(mg/L) | 1.3515.33 | 112.56339.00 | 15.1148.36 | 115.88345.6. | 53.18176.66 | 95.32220.78 | 6.8528.69 | 87.22345.12 |
实例2：试验方法同实例1，补骨脂定、福美胂、银泰、甲基托布津、多菌灵6种原药对苹果腐烂病菌菌丝生长的抑制作用如下：
5种药剂对苹果腐烂病菌菌丝生长的抑制作用
| 抑制作用 | 补骨脂定 | 福美胂 | 银泰 | 甲基托布津 | 多菌灵 |
| EC50(mg/L)EC90(mg/L) | 1.3515.33 | 8.93128.98 | 26.1893.37 | 9.16147.85 | 1.4726.86 |
Claims (2)
1.用补骨脂定防治植物真菌病害的生物农药，其特征在于化合物是干燥补骨脂种子5Kg，用70％工业乙醇冷浸提取四次，浸出液减压浓缩得乙醇提取物，加水混溶，依次用石油醚、乙酸乙酯萃取；取乙酸乙酯萃取部位浸膏100g，经反复硅胶柱层析，用石油醚一乙酸乙酯梯度洗脱，分离得到补骨脂定(50mg)(石油醚∶乙酸乙酯＝4∶1部分)。
2.根据权利要求1所述的用补骨脂定防治植物真菌病害的生物农药，其特征在于所述的补骨脂定，无色针晶，mp 290～292℃；TLC检查在紫外下为蓝荧光斑点；ESIMS：m/z 337.4，[M+H]+，335.3[M-H]；HNMR(COCD3+CDCl3)：1.83(3H，s，CH3)，1.82(3H，s，CH3)，3.42(2H，d，J＝7.2Hz，H-1′)，5.41(1H，m，H-2′)，6.92(1H，s，H-4)，6.96(1H，dd，J＝1.9，8.4Hz，H-8)，7.13(1H，d，J＝1.8Hz，H-10)，7.68(1H，s，H-1)，7.82(1H，d，J＝8.4Hz，H-7)；
补骨脂定结构式：
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100100165A CN101213980B (zh) | 2008-01-02 | 2008-01-02 | 用补骨脂定防治植物真菌病害的生物农药 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100100165A CN101213980B (zh) | 2008-01-02 | 2008-01-02 | 用补骨脂定防治植物真菌病害的生物农药 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101213980A true CN101213980A (zh) | 2008-07-09 |
| CN101213980B CN101213980B (zh) | 2011-05-18 |
Family
ID=39620527
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100100165A Active CN101213980B (zh) | 2008-01-02 | 2008-01-02 | 用补骨脂定防治植物真菌病害的生物农药 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101213980B (zh) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067850A (zh) * | 2011-03-02 | 2011-05-25 | 江苏省中国科学院植物研究所 | 补骨脂素作为农业杀菌剂或杀虫剂的用途 |
| CN105061197A (zh) * | 2015-08-28 | 2015-11-18 | 林天样 | 一种新的三萜化合物及其制备方法和医药用途 |
| CN105294180A (zh) * | 2014-06-25 | 2016-02-03 | 于海峰 | 一种天然植物营养液及其应用 |
| CN106135281A (zh) * | 2016-06-24 | 2016-11-23 | 叶松 | 一种防治葡萄虎蛾的组合物及其制备方法 |
| CN106804629A (zh) * | 2017-01-17 | 2017-06-09 | 长江大学 | 对节白蜡中具有杀菌活性的提取物及其杀菌组合物 |
| CN110710538A (zh) * | 2019-11-04 | 2020-01-21 | 江苏丘陵地区镇江农业科学研究所 | 含补骨脂种子提取物和丙硫菌唑的复合杀菌组合物、杀菌剂及应用 |
| CN110973167A (zh) * | 2019-11-30 | 2020-04-10 | 陕西康禾立丰生物科技药业有限公司 | 一种含补骨脂种子提取物的农药组合物 |
| CN110999922A (zh) * | 2019-12-25 | 2020-04-14 | 陕西康禾立丰生物科技药业有限公司 | 一种含补骨脂种子提取物的农药组合物 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843127A (zh) * | 2006-05-16 | 2006-10-11 | 沈阳化工学院 | 一种植物源农用杀菌剂 |
-
2008
- 2008-01-02 CN CN2008100100165A patent/CN101213980B/zh active Active
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067850A (zh) * | 2011-03-02 | 2011-05-25 | 江苏省中国科学院植物研究所 | 补骨脂素作为农业杀菌剂或杀虫剂的用途 |
| CN102067850B (zh) * | 2011-03-02 | 2013-07-17 | 江苏省中国科学院植物研究所 | 补骨脂素作为农业杀菌剂或杀虫剂的用途 |
| CN105294180A (zh) * | 2014-06-25 | 2016-02-03 | 于海峰 | 一种天然植物营养液及其应用 |
| CN105294180B (zh) * | 2014-06-25 | 2018-11-13 | 于海峰 | 一种天然植物营养液及其应用 |
| CN105061197A (zh) * | 2015-08-28 | 2015-11-18 | 林天样 | 一种新的三萜化合物及其制备方法和医药用途 |
| CN106135281A (zh) * | 2016-06-24 | 2016-11-23 | 叶松 | 一种防治葡萄虎蛾的组合物及其制备方法 |
| CN106804629A (zh) * | 2017-01-17 | 2017-06-09 | 长江大学 | 对节白蜡中具有杀菌活性的提取物及其杀菌组合物 |
| CN110710538A (zh) * | 2019-11-04 | 2020-01-21 | 江苏丘陵地区镇江农业科学研究所 | 含补骨脂种子提取物和丙硫菌唑的复合杀菌组合物、杀菌剂及应用 |
| CN110710538B (zh) * | 2019-11-04 | 2021-06-25 | 江苏丘陵地区镇江农业科学研究所 | 含补骨脂种子提取物和丙硫菌唑的复合杀菌组合物、杀菌剂及应用 |
| CN110973167A (zh) * | 2019-11-30 | 2020-04-10 | 陕西康禾立丰生物科技药业有限公司 | 一种含补骨脂种子提取物的农药组合物 |
| CN110999922A (zh) * | 2019-12-25 | 2020-04-14 | 陕西康禾立丰生物科技药业有限公司 | 一种含补骨脂种子提取物的农药组合物 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101213980B (zh) | 2011-05-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101213980B (zh) | 用补骨脂定防治植物真菌病害的生物农药 | |
| Marques et al. | Crop irrigation (soybean, bean, and corn) with enrofloxacin-contaminated water leads to yield reductions and antibiotic accumulation | |
| Kalhoro et al. | Fungicidal properties of ginger (Zingiber officinale) essential oils against Phytophthora colocasiae | |
| CN101215229A (zh) | 抗植物真菌病害的补骨脂查尔酮类化合物 | |
| Behiry et al. | Urtica dioica and Dodonaea viscosa leaf extracts as eco-friendly bioagents against Alternaria alternata isolate TAA-05 from tomato plant | |
| CN101215278B (zh) | 抗植物真菌活性补骨脂二氢黄酮 | |
| CN101219932B (zh) | 补骨脂酚用于防治植物病害的用途 | |
| CN101703053B (zh) | 一种含有噁唑菌酮的杀菌组合物 | |
| CN102464624A (zh) | 一种用于防治植物病原线虫的化合物及方法 | |
| CN101703057B (zh) | 一种含噁唑菌酮的农用杀菌组合物 | |
| CN110742076B (zh) | 甘草苷在防治作物卵菌及真菌病害中的用途 | |
| Bibi et al. | Effect of culture filtrates of Alternaria alternata on seed germination and seedling growth of sesame | |
| CN107136073A (zh) | 一种含有大黄素甲醚与甲氧基丙烯酸酯类杀菌剂组合物 | |
| CN104351180A (zh) | 一种用于防治水稻稻曲病的微胶囊悬浮剂 | |
| CN107771866A (zh) | 一种茼蒿抑菌活性单体及其使用方法 | |
| Nadeem et al. | Novel development of nutritive, enhanced bioactivities, antioxidant, antimicrobial, antiparasitic, DNA protection and new insights | |
| CN105191997A (zh) | 一种含有咪鲜胺和黄芩的杀菌剂 | |
| CN100425141C (zh) | 一种氯胺磷化合物的应用 | |
| Moradi et al. | Biosynthesized silver nanoparticles using Trichoderma harzianum reduce charcoal rot disease in bean | |
| CN104488951A (zh) | 一种防治爪哇根结线虫的药物组合物 | |
| Harjan et al. | Antifungal Activity of Crude and Phenolic Extract to Rice Crusts and Chemical Pesticide (Blitinute) in Inhibition of Fungi Isolate from Rice Seeds | |
| Bloch et al. | Nanobiotechnology of the plant microbiome | |
| KR101104863B1 (ko) | 하고초 추출물을 유효성분으로 함유하는 식물병 방제용 조성물 및 이를 이용한 식물병 방제방법 | |
| CN107041369A (zh) | 一种防治马铃薯晚疫病的杀菌组合物 | |
| CN105309461A (zh) | 一种含有咪鲜胺和肉桂醛的杀菌剂 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 110142 Shenyang economic and Technological Development Zone, Liaoning, No. 11 Patentee after: SHENYANG University OF CHEMICAL TECHNOLOGY Address before: 110142 Shenyang economic and Technological Development Zone, Liaoning, No. 11 Patentee before: Shenyang Institute of Chemical Technology |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20160418 Address after: Xinghua street Tiexi District of Shenyang City, Liaoning Province, No. 110021 58-3 Patentee after: Shenyang Feng Yiyuan Technology Co.,Ltd. Address before: 110142 Shenyang economic and Technological Development Zone, Liaoning, No. 11 Patentee before: Shenyang University of Chemical Technology |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170524 Address after: 110027 -6, No. eight East 9 Shen Shen road, Shenyang economic and Technological Development Zone, Liaoning, China Patentee after: Shenyang same Agricultural Chemical Co.,Ltd. Address before: Xinghua street Tiexi District of Shenyang City, Liaoning Province, No. 110021 58-3 Patentee before: Shenyang Feng Yiyuan Technology Co.,Ltd. |
|
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 110027 -6, eight East Road, Shen Shen Xi, Shenyang economic and Technological Development Zone, Liaoning, 9 Patentee after: Shenyang with the same biological pesticide Co.,Ltd. Address before: 110027 -6, No. eight East 9 Shen Shen road, Shenyang economic and Technological Development Zone, Liaoning, China Patentee before: Shenyang same Agricultural Chemical Co.,Ltd. |