CN101210054A - Method for extracting high transparent xanthan gum - Google Patents

Abstract

The invention discloses an extraction method for high transparent xanthan gum. Ethanol is added to the zymotic fluid of xanthan gum to carry out once extraction; dehydration is carried out by a filter to separate out the xanthan gum; the filtrate thereof is reclaimed in an alcohol distillation tower directly; the extracted xanthan gum is diluted with water, and then is added with alkali protease and lysozyme to carry out bienzymatic hydrolysis; next, the hydrolyzate solution is added with ethanol for second extraction after being processed by high-temperature enzyme removing treatment, the high transparent xanthan gum product is obtained by separation and purification.

Description

A kind of extracting method of high transparent xanthan gum

Technical field: the invention belongs to the xanthan gum production technical field, be specifically related to a kind of extracting method of high transparent xanthan gum.

Background technology: xanthan gum (Xanthan gum) is that the yellow unit cell of cabbage black rot bird rape (Xanthomonascampestris) is main raw material with the W-Gum, and mixed polysaccharide outside the acid born of the same parents of a kind of microorganism that heat aerobic organisms fermentation produces abbreviates XC usually as.Xanthan gum is the xanthan gum that present best performance is in the world got over, its solution is a kind of non-Newtonian fluid, compare with other high biological polymer, have unique physical and chemical properties and full functionality, be often used as thickening material, emulsifying agent, suspension agent, stablizer, be widely used in more than 20 fields such as food, medicine, daily-use chemical industry, oil recovery, weaving, pottery, printing and dyeing, have market potential.The research and the production of China's xanthan gum industry are started late, the later stage seventies just begins to study, begin suitability for industrialized production in the initial stage eighties, and production technique is backward relatively, therefore quality product is relatively weaker with the throughput of products such as the fine chemistry industry level xanthan gum with bigger gap, especially high added value of abroad going together, transparent type xanthan gum.Because the technology-intensive degree of xanthan gum is higher, the through engineering approaches difficulty is big, the present domestic large-scale xanthan gum manufacturer of several families that only has.

Xanthan gum is produced and is comprised fermentation and downstream processing two portions, comparatively speaking, less to the downstream processing aspect research that xanthan gum is produced, and downstream such as separation and purification processes required expense and accounts for the over half of production cost, the downstream processing charges of high-quality xanthan gum such as especially high transparent type xanthan gum and instantly-soluble xanthan gum is higher, and its import price is more than the twice of domestic food level xanthan gum price.Therefore the downstream processing technology has become the critical process that xanthan gum is produced, and processing technology is the diversified essential means of preparing product, also is the important factor of decision product quality and cost.

Except containing xanthan gum, also contain mycelium, residual sugar, inorganic salt and other impurity in the xanthan gum fermentation broth, different extraction and separation method will determine performance, quality and the purposes of xanthan gum.At present, the processing method of xanthan gum product separation and Extraction mainly contains direct drying method, ultra-filtration membrane partition method, organic solvent precipitation method and salt precipitation method etc., but on using, all be subjected to the restriction of condition separately, the specific aim that so how to improve product has become the key issue that present domestic xanthan gum industry is faced to improve product competitiveness, to increase economic efficiency.

Separation for xanthan gum is purified, traditional organic solvent deposit technical process (shown in Figure 1 as Figure of description): fermented liquid is after the pre-treatment dilution, carrying out ultrafiltration process concentrates, add a certain proportion of alcohol then and carry out washing precipitation, again through making finished product after solid-liquid separation, vacuum-drying, the pulverizing.But the xanthan gum of this kind method preparation has following shortcoming:

1, alcohol to oil ratio is big, causes extraction expense height after the xanthan gum;

2, in the preprocessing process, need dilute with water, cause the final xanthan gum wastewater flow rate that produces to increase, and xanthan gum waste water remains one of reluctant sewage so far;

3, the quality of finished product xanthan gum is low, thalline content height wherein, and impurity is more;

4, ultrafiltration effect is relatively good, but cost is very high;

Because traditional organic solvent deposit technology has above shortcoming, therefore cause the quality of domestic xanthan gum to be difficult to get a promotion.

Summary of the invention: the purpose of this invention is to provide a kind of extracting method of high transparent xanthan gum, to overcome the deficiencies in the prior art.

The extracting method of high transparent xanthan gum of the present invention is finished by following processing step:

1) once extracts: in xanthan gum fermentation broth, add ethanol, alcohol concn in the control solution is 50～70%, after stirring 30～90min, dewater by filter and to isolate xanthan gum, it is a leaching process of xanthan gum, can reduce the foreign matter content in the subsequent handling, its filtrate directly goes the alcohol distillation tower to reclaim;

2) viscosity of xanthan gums after once extracting dilution process: because above-mentioned steps 1) is bigger, needs water to dilute, and control dissolving back xanthan gum concentration 0.5～3%;

3) the xanthan gum heating double enzymolysis method: with step 2) after the dilution also remains on 30～60 ℃, regulating the pH value is 7.5～8.0, add Sumizyme MP then, continue to stir 1～2 hour, stablized 1～5 hour, the enzymolysis solution of gained is cooled to 20 ℃, regulate pH7.0, add N,O-Diacetylmuramidase then, continued stir process 1～2 hour, because Sumizyme MP can generate polypeptide or amino acid by protein hydrolysate molecule peptide chain, ability with stronger decomposing protein, and N,O-Diacetylmuramidase claims muramidase, is a kind of lytic enzyme that acts on microorganism wall specially, because of it has bacteriolysis, so the called after N,O-Diacetylmuramidase and since bacteria cell wall to the susceptibility of N,O-Diacetylmuramidase than higher, therefore obtained to use widely, the double enzymolysis method can be removed insoluble substances such as tropina residual in the fermented liquid, makes xanthan gum have purity preferably, transparence and viscosity;

4) enzyme that goes out is handled: the solution through double enzymolysis in the step 3) is heated to 80～100 ℃, is incubated 20～60 minutes;

5) second extraction: the solution in the step 4) is cooled to 40 ℃, adds ethanol again, the alcohol concn in the control solution mixes to stir and carried out second extraction in 30～90 minutes 70%～90%;

6) separate purification: second extraction solution in the step 5) is carried out solid-liquid separation by filter, and filtrate is directly removed abstraction process one time, and solid drying, pulverizing back obtain the high transparent xanthan gum product.

The extracting method of high transparent xanthan gum of the present invention, preparation technology is simple, easy to operate, extraction cost is low, productive rate is high, transmittance is high, at present, the high transparent xanthan gum product of external preparation, content is that 1% the transmittance of xanthan gum solution when wavelength is 600nm is generally 85%, and the prepared high transparent xanthan gum product of the present invention, content is that 1% the transmittance of xanthan gum solution when wavelength is 600nm is higher than 87%.

Description of drawings: Fig. 1 is traditional xanthan gum extraction process schema;

Fig. 2 is a high transparent xanthan gum extraction process schema of the present invention.

Embodiment:

Embodiment 1, from xanthan gum fermentation broth, extract the high transparent xanthan gum method and finish by following processing step:

1) once extract: add ethanol in xanthan gum fermentation broth, the alcohol concn in the control solution is 50%, stir 30min after, dewater by filter and to isolate xanthan gum, its filtrate directly goes the alcohol distillation tower to reclaim;

2) dilution process: the xanthan gum water that step 1) is extracted dilutes, and control dissolving back xanthan gum concentration 0.5%;

3) xanthan gum double enzymolysis method: with step 2) after the dilution is heated to 40 ℃, and insulation, regulating the pH value is 7.5, add Sumizyme MP then, continue to stir 1 hour, stablized 1 hour, the enzymolysis solution of gained is cooled to 20 ℃, regulate pH7.0, add N,O-Diacetylmuramidase then, continued stir process 1 hour;

4) enzyme that goes out is handled: the solution through double enzymolysis in the step 3) is heated to 80 ℃, is incubated 20 minutes;

5) second extraction: the solution in the step 4) is cooled to 40 ℃, adds ethanol again, the alcohol concn in the control solution mixes to stir and carried out second extraction in 30 minutes 70%%;

6) separate purification: second extraction solution in the step 5) is carried out solid-liquid separation by filter, and filtrate is directly removed abstraction process one time, promptly gets the high transparent xanthan gum product after solid drying, the pulverizing.

Embodiment 2, according to embodiment 1, from xanthan gum fermentation broth, extract the high transparent xanthan gum method and finish by following processing step:

1) once extract: add ethanol in xanthan gum fermentation broth, the alcohol concn in the control solution is 70%, stir 90min after, dewater by filter and to isolate xanthan gum, its filtrate directly goes the alcohol distillation tower to reclaim;

2) dilution process: the xanthan gum water that step 1) is extracted dilutes, and control dissolving back xanthan gum concentration 3%;

3) xanthan gum double enzymolysis method: with step 2) after the dilution is heated to 60 ℃, and insulation, regulating the pH value is 8.0, add Sumizyme MP then, continue to stir 2 hours, stablized 5 hours, the enzymolysis solution of gained is cooled to 20 ℃, regulate pH7.0, add N,O-Diacetylmuramidase then, continued stir process 2 hours;

4) enzyme that goes out is handled: the solution through double enzymolysis in the step 3) is heated to 100 ℃, is incubated 60 minutes;

5) second extraction: the solution in the step 4) is cooled to 40 ℃, adds ethanol again, the alcohol concn in the control solution mixes to stir and carried out second extraction in 90 minutes 90%%;

6) separate purification: second extraction solution in the step 5) is carried out solid-liquid separation by filter, and filtrate is directly removed abstraction process one time, promptly gets the high transparent xanthan gum product after solid drying, the pulverizing.

At present, Shandong Fufeng Fermentation Co.,Ltd produces 20000 tons of xanthan gum per year, after adopting novel technology for extracting of the present invention, the profit of xanthan gum per ton can increase by 4.5 ten thousand yuan, 900,000,000 yuan of year new profits, economic benefit is extremely obvious, and for improving our domestic product specific aim, the competitive power that strengthens xanthan gum product all is significant.

Claims (1)

1. the extracting method of a high transparent xanthan gum is characterized in that being finished by following processing step:

1) once extract: add ethanol in xanthan gum fermentation broth, the alcohol concn in the control solution is 50～70%, stir 30～90min after, dewater by filter and to isolate xanthan gum, its filtrate directly goes the alcohol distillation tower to reclaim;

2) dilution process: the xanthan gum water that step 1) is extracted dilutes, and control dissolving back xanthan gum concentration 0.5～3%;

3) the xanthan gum heating double enzymolysis method: with step 2) after the dilution also remains on 30～60 ℃, regulating the pH value is 7.5～8.0, add Sumizyme MP then, continue to stir 1～2 hour, stablized 1～5 hour, the enzymolysis solution of gained is cooled to 20 ℃, regulate pH7.0, add N,O-Diacetylmuramidase then, continued stir process 1～2 hour;

4) enzyme that goes out is handled: the solution through double enzymolysis in the step 3) is heated to 80～100 ℃, is incubated 20～60 minutes;

5) second extraction: the solution in the step 4) is cooled to 40 ℃, adds ethanol again, the alcohol concn in the control solution mixes to stir and carried out second extraction in 30～90 minutes 70%～90%;

6) separate purification: second extraction solution in the step 5) is carried out solid-liquid separation by filter, and filtrate is directly removed abstraction process one time, and solid drying, pulverizing back obtain the high transparent xanthan gum product.

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