CN101209356B - Method for preparing medical knee joint semilunar cartilage - Google Patents
Method for preparing medical knee joint semilunar cartilage Download PDFInfo
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- CN101209356B CN101209356B CN 200710192209 CN200710192209A CN101209356B CN 101209356 B CN101209356 B CN 101209356B CN 200710192209 CN200710192209 CN 200710192209 CN 200710192209 A CN200710192209 A CN 200710192209A CN 101209356 B CN101209356 B CN 101209356B
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- meniscus
- knee joint
- silkworm silk
- silk
- fibroin fiber
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Abstract
The invention relates to a preparation method of a medical knee joint meniscus, the steps mainly include that: silk with the high quality is firstly selected to be crocheted into a silk meniscus whichtakes a shape of wedge, the outer edge of the silk meniscus is thicker than the inner edge, and the medical knee joint meniscus can be prepared by degumming, drying, silk protein fiber-collagen compound shaping and other steps. The invention has the advantages of convenient production, low cost and so on, when in use, the transplantation operation is simple, convenient, safe and reliable, the tolerance of patients is good and the immunogenicity is small. As the silk protein fiber after the removal of sericin has good mechanical properties and biocompatibility, the invention can bear the functions of knee joint stabilization, load conduction, articular cartilage protection and lubrication of the natural meniscus at the early stage of the transplantation, at the same time, the invention hasthe function of a tissue engineering scaffold, and can support the cell growth and blood vessel growth at the advanced stage of the transplantation and be gradually degraded and absorbed along with theregeneration of the real natural meniscus, so the invention provides a new choosing path for the treatment of the patients with the seriously damaged and apraxia knee joint meniscus.
Description
Technical field
The present invention relates to a kind of medical meniscal preparation method, be specifically related to a kind of medical degradable, the preparation method of absorbable meniscus of knee joint, belong to the prosthetic material field.
Background technology
Meniscus is the fibrous cartilage that is arranged in knee joint, has to absorb concussion, transfer charge, nutrition articular cartilage, lubricated and increase the joint contact area and keep critical functions such as the joint is stable.Meniscal outer rim plumpness, inner edge is sharp thin, and the blood supply of peripheral part is still abundant, and remainder blood lacks repairing regenerating ability for poor.Follow-up investigation finds that the size of meniscus cut-out and the onset risk of knee joint osseous arthritis are closely related.After the excision of meniscus major injury, meniscus quickens, causes developing of instability of knee and osteoarthritis to protective effect forfeiture, the articular cartilage degeneration of articular cartilage.Along with to the going deep into of first quarter moon plate structure and function cognition, the viewpoint that meniscus can not lack gains public acceptance.For can't keeping meniscal patient, meniscal transplanting or reproduce to repair and become the problem that people pay close attention to the most.Reproduce at present and repair the emphasis that the meniscal 26S Proteasome Structure and Function of severe injury has become Chinese scholars research.
At present, be used for the meniscal implantation tissue engineering material and comprise that natural biologic material and synthetic biomaterial mainly contain: 1. polyvinyl alcohol-hydrogel (PVA-H); 2. gelfoam; 3. collagen protein; 4. alginate; 5. polyglycolic acid (PGA); 6. polylactic acid (PLA); 7. small intestinal submucosa tissue; 8. polyglycolic acid and copolymer of poly lactic acid; 9. fibrin gel etc.; But the artificial meniscus of these materials histocompatibility, prosthese IA fixing, the problem aspect the degradation under material aging, wearing and tearing, the biomechanical property is appearring under the situation of the power that is squeezed, shears, extends for a long time, its application prospect fascination or limited.
Taking from soma, to rebuild meniscus be that new approach has been opened up in the meniscus injury treatment, and this method draws materials conveniently, need not sterilization, and right clinical effectiveness remains to be inquired into; And the allogeneic meniscal implantation exist the source not enough, for meniscus size and form between receptor there are differences, immunological rejection, potential pathophoresis risk etc., thereby also limited its clinical practice.
Summary of the invention
The objective of the invention is to damage apraxia, a kind of medical meniscal degradable of alternative damage, the preparation method of absorbable medical knee joint semilunar cartilage are provided for solving patient's meniscus of knee joint.
Medical knee joint semilunar cartilage of the present invention, its preparation method mainly may further comprise the steps:
A), braiding silkworm silk meniscus: select the high-quality silkworm silk of suitable length for use, the silkworm silk crocheting at middle part is become the outer rim silkworm silk meniscus that is the contract shape thicker than inner edge, the silkworm silk that two is reserved is antilift wire;
B), the drying of coming unstuck: the silkworm silk meniscus is placed 0.5% sodium carbonate (Na
2CO
3) be heated to 100 ℃ of lasting half an hour in the solution, take out with behind the water rinse, place 0.5% sodium carbonate (Na of preparation again again
2CO
3) be heated to 100 ℃ of lasting half an hour in the solution, take out with water rinse, drying, silkworm silk is come unstuck be the fibroin fiber meniscus;
C), fibroin fiber-collagen composite setting: place the meniscus of knee joint mould to inject type i collagen solution the fibroin fiber meniscus, place 4 ℃ of refrigerators to leave standstill a few hours earlier, type i collagen solution is fully immersed in the fibroin fiber meniscus, change over to again in-80 ℃ of refrigerators and leave standstill typing, take out the demoulding then, change over to again in the freezer dryer after the drying, be the compound meniscus of fibroin fiber-collagen;
D), packing and storing: the compound meniscus of fibroin fiber-collagen is medical knee joint semilunar cartilage with the individual packaging storage of ethylene oxide gas sterilization back.
Medical knee joint semilunar cartilage with method preparation of the present invention has the following advantages:
1, provides a kind of new selection approach for treating clinical meniscus injury, be particularly useful for the patient of the badly damaged apraxia of meniscus of knee joint, filled up the blank in this field.
2, the artificial meniscus prosthese of fibroin fiber-collagen-based composite is to integrate fibroin fiber and collagen, in line with the thought of learning from other's strong points to offset one's weaknesses, make the artificial meniscus of fibroin fiber-collagen-based composite on mechanics, satisfy the meniscus requirement, fibroin fiber after coming unstuck is as natural macromolecular material, possess the good mechanical performance, aspect biocompatibility, be better than traditional synthetic degradable high polymer material simultaneously again.Transplanting the early stage meniscus of just bearing to kneed protection and the lubrication of stablizing, loading conduction, articular cartilage.Possess simultaneously the effect of tissue engineering bracket again, transplant the later stage again can the sustenticular cell growth and blood vessel grow into, and be accompanied by the regeneration of genuine natural meniscus and degraded and absorbed gradually.
3, the proteic knitting arrangement of the artificial meniscus prosthese of fibroin fiber-collagen-based composite silk fiber can play tension, resistance to compression, anti-shearing effect, and collagen can be kept its form and be convenient to the histiocyte effect of growing into.When braiding, reserve antilift wire, fixing when being convenient to the prosthese transplanting.
4, also have prosthese transplant simple and convenient, safe and reliable, advantage such as patient tolerability is good, and immunogenicity is little and convenient for production, with low cost, be easy to popularize.
Description of drawings
Fig. 1 is a kind of structural representation of medical knee joint semilunar cartilage of the present invention;
Fig. 2 is a medical knee joint semilunar cartilage of the present invention structural representation when implanting knee joint.
The specific embodiment
The present invention is further illustrated for following structure accompanying drawing.
Referring to accompanying drawing 1,1 among the figure is the meniscal outer rim of silkworm silk, the 2nd, and the meniscal inner edge of silkworm silk, the 3rd, the meniscal antilift wire of silkworm silk.During making, select high-quality silkworm silk earlier for use, the silkworm silk crocheting at middle part is become the outer rim 1 silkworm silk meniscus that be contract shape thicker than inner edge 2, the silkworm silk that two is reserved is antilift wire 3.Then the silkworm silk meniscus is placed 0.5% sodium carbonate (Na
2CO
3) be heated to 100 ℃ of lasting half an hour in the solution, take out with behind the water rinse, place 0.5% sodium carbonate (Na of preparation again again
2CO
3) be heated to 100 ℃ of lasting half an hour in the solution, take out with water rinse, drying, silkworm silk is come unstuck be the fibroin fiber meniscus.Again the fibroin fiber meniscus is placed the mould identical to inject type i collagen solution with the meniscus of knee joint shape, place 4 ℃ of refrigerators to leave standstill a few hours earlier, type i collagen solution is fully immersed in the fibroin fiber meniscus, change over to again in-80 ℃ of refrigerators and leave standstill typing, take out the demoulding then, change over to again in the freezer dryer after the drying, be the compound meniscus of fibroin fiber-collagen.At last the compound meniscus of fibroin fiber-collagen is stored with ethylene oxide gas sterilization back individual packaging and be medical knee joint semilunar cartilage.
During use, medical knee joint semilunar cartilage is installed on the correspondence position of the natural meniscus of damaging apraxia of taking-up, meniscal outer rim 1 thicker knee joint 6 outsides that place, thin knee joint 6 inboards that place of meniscal inner edge 2, as shown in Figure 2, after the antilift wire 3 at meniscal two passes default osseous tunnel 4 and tension, with can upwards screwing in the osseous tunnel 4 along the osseous tunnel lower port by absorption screw 5, antilift wire 3 extruding can be fixed in the osseous tunnel 4, the also available wire discontinuous sewing of meniscus outer rim 1 can be finished the replacement of medical knee joint semilunar cartilage on joint capsule.
Medical knee joint semilunar cartilage with this method makes has the advantages such as convenient for production, with low cost, and during use, transfer operation is simple and convenient, and is safe and reliable, and patient tolerability is good, and immunogenicity is little. Silk after owing to come unstuck is as natural macromolecular material; have good mechanical property and biocompatibility; transplanting early stage protection and the lubrication of stablizing, loading conduction, articular cartilage kneed of just bearing; transplant the later stage again can the supportint cell growth and blood vessel grow into, and be accompanied by the regeneration of natural meniscus and be degraded and absorbed gradually. For the patient's of the badly damaged appraxia of meniscus of knee joint treatment provides a kind of new selection approach.
Claims (1)
1. the preparation method of a medical knee joint semilunar cartilage is characterized in that may further comprise the steps:
A), braiding silkworm silk meniscus: select the high-quality silkworm silk of suitable length for use, the silkworm silk crocheting at middle part is become the outer rim silkworm silk meniscus that is the contract shape thicker than inner edge, the silkworm silk that two is reserved is antilift wire;
B), the drying of coming unstuck: the silkworm silk meniscus is placed 0.5% sodium carbonate (Na
2CO
3) be heated to 100 ℃ of lasting half an hour in the solution, take out with behind the water rinse, place 0.5% sodium carbonate (Na of preparation again again
2CO
3) be heated to 100 ℃ of lasting half an hour in the solution, take out with water rinse, drying, silkworm silk is come unstuck be the fibroin fiber meniscus;
C), fibroin fiber-collagen composite setting: place the meniscus of knee joint mould to inject type i collagen solution the fibroin fiber meniscus, place 4 ℃ of refrigerators to leave standstill a few hours earlier, type i collagen solution is fully immersed in the fibroin fiber meniscus, change over to again in-80 ℃ of refrigerators and leave standstill typing, take out the demoulding then, change over to again in the freezer dryer after the drying, be the compound meniscus of fibroin fiber-collagen;
D), packing and storing: the compound meniscus of fibroin fiber-collagen is medical knee joint semilunar cartilage with the individual packaging storage of ethylene oxide gas sterilization back.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710192209 CN101209356B (en) | 2007-12-21 | 2007-12-21 | Method for preparing medical knee joint semilunar cartilage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710192209 CN101209356B (en) | 2007-12-21 | 2007-12-21 | Method for preparing medical knee joint semilunar cartilage |
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| CN101209356A CN101209356A (en) | 2008-07-02 |
| CN101209356B true CN101209356B (en) | 2010-04-21 |
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| CN 200710192209 Expired - Fee Related CN101209356B (en) | 2007-12-21 | 2007-12-21 | Method for preparing medical knee joint semilunar cartilage |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8771353B2 (en) | 2011-03-08 | 2014-07-08 | DePuy Synthes Products, LLC | Method and implant for replacing damaged meniscal tissue |
| CN107669373B (en) * | 2017-10-24 | 2019-11-01 | 北京航空航天大学 | A kind of artificial Meniscus scaffold and its weaving method based on fiber weave structure |
| CN110354309A (en) * | 2019-07-15 | 2019-10-22 | 北京大学第三医院（北京大学第三临床医学院） | A kind of method of the bionical meniscus of 3D printing |
| CN113633827A (en) * | 2021-08-16 | 2021-11-12 | 中国人民解放军总医院第四医学中心 | Silk woven meniscus implant and preparation method thereof |
| CN114343925A (en) * | 2022-01-07 | 2022-04-15 | 中国科学院长春应用化学研究所 | Artificial meniscus and manufacturing method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895687A (en) * | 2006-06-09 | 2007-01-17 | 浙江大学 | Netted tissue-engineering stand |
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2007
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895687A (en) * | 2006-06-09 | 2007-01-17 | 浙江大学 | Netted tissue-engineering stand |
Non-Patent Citations (1)
| Title |
|---|
| 江莹.组织工程韧带的初步研究.中国优秀硕士学位论文全文数据库.2007,全文. * |
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