CN101208153B - Device and method for separating magnetic particles - Google Patents
Device and method for separating magnetic particles Download PDFInfo
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- CN101208153B CN101208153B CN2006800227729A CN200680022772A CN101208153B CN 101208153 B CN101208153 B CN 101208153B CN 2006800227729 A CN2006800227729 A CN 2006800227729A CN 200680022772 A CN200680022772 A CN 200680022772A CN 101208153 B CN101208153 B CN 101208153B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/035—Open gradient magnetic separators, i.e. separators in which the gap is unobstructed, characterised by the configuration of the gap
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/22—Details of magnetic or electrostatic separation characterised by the magnetical field, special shape or generation
Abstract
The invention relates to a method and device for separating magnetic particles, for separating magnetic particles from a sample housed in an inner space of the separating device. In accordance with the invention, the magnetic field is generated with a specific configuration of the magnets (3). This specific configuration enables devices of different sizes with a reduced number of magnets or types of magnets to be established.
Description
Technical field
The present invention relates to the technical field that magnetic particle separates.
Background technology
The separation of dissimilar particles has many application.For example, in pharmacy, biology and area of pharmacology, often need isolate the composition of determining (for example definite type of antibody) of for example sample, suspension or solution so that analyze the problem (for example so that diagnose the illness) relevant with these compositions.Traditional such separation method that is used for realizing composition, particle or molecule is separation method and the centrifugal process by affinity column (affinity columns).
The another kind of method that is widely used in recent years is based on the separation method that utilizes magnetic particle.This method can fast and be easy to be used for accurate and reliable component separation, be used for such as, specific proteins, inhereditary material and biomolecule (referring to, for example, Z M Saiyed, et al., " Application of Magnetic Techniques in the Field of DrugDiscovery and Biomedicine ", BioMagnetic Research and Technology 2003, I:2, published 18September 2003[available at http://www.biomagres.com/content/1/1/2]).This method is based on the utilization of magnetic particle, magnetic particle with will from sample, solution, suspension etc., isolated special composition in some acceptors (recipient) or analog, combine.By applying magnetic field, magnetic particle and remaining sample separation, or rather, magnetic particle is concentrated in a part of acceptor, when removing remaining sample (or the major part of remaining sample) at least, they are retained in there (for example, because the effect in the magnetic field that applies).Next, can make reserve part stand cleaning treatment, this cleaning treatment can comprise another separation of magnetic particle etc.
U.S. Pat 4910148A and International Patent Application WO 02/055206A disclose two kinds of piece-rate systems based on magnetic particle.These two kinds of systems utilize magnet to combine with sample to attract magnetic particle substantially, so as they can with remaining sample separation.
Two types of magnetic particles are arranged.First kind is those of permanent magnetization, for example magnet.These particles are characterised in that in fact they have and external magnetic induction (B) irrelevant constant magnetic moment (m).For this a series of particle, the power that is applied on them can be expressed as:
Second type particle has the intensity of magnetization that changes according to the external magnetic field.For middle high field, can suppose the magnetic susceptibility substantially constant.This serial particle comprises soft ferromagnetic, paramagnetic and superparamagnetic material.Utilize this approximation, the power that imposes on particle can be expressed as:
Wherein χ is a magnetic susceptibility, the relation between its expression external magnetic field and the magnetic moment.
From these expression formulas, as can be seen, have at least dual mode can improve the efficient (being applied to power on the particle) of separating magnetic particles method, that is: by increase
-by increasing magnetic susceptibility and/or magnetic moment; Or
-by producing big space varying magnetic field.
Other characteristics that are closely related with its biological function that increase magnetic susceptibility and/or magnetic moment and do not influence magnetic particle are not simple task.Yet, based on the system of the utilization of the non-uniform magnetic-field in the sample area or theoretic at least system realized good with effectively separate.
U.S. Pat 6361749B discloses a kind of separator with magnet of North and South direction distribution, and wherein the quantity of magnet equals the quantity of magnetic pole.But, this structure has shortcoming, because when the quantity of magnetic pole greater than four the time, in fact center at sample does not exist magnetic gradient, this is that the particle at sample acceptor center is not moving or (utilizing four magnets to produce under the situation of four magnetic poles to the moving reason of acceptor wall shift lentamente to the acceptor wall shift, though have gradient at the center, in zone distortion is arranged also near magnet, this is more detailed description below).
U.S. Pat 5705064A discloses the separator that a kind of cylinder that is formed by magnet ring constitutes, wherein on the cross section of cylinder, each magnet have two with adjoin or the respective side of adjacent magnet is parallel and the side by placing mutually.The direction of magnetization of magnet is followed the angular progression of Δ γ=2 Δ θ, and (wherein Δ γ is illustrated in the cross section of described cylinder the variation of the direction of magnetization between a magnet and the adjacent magnet, and Δ θ is illustrated in the cross section of described cylinder the variation of the angle position between a magnet and the adjacent magnet) (perhaps, describe in another way, the angular progression of γ=2 θ, here γ is illustrated in the cross section of described cylinder the magnetization angle direction with respect to the magnet of the dipole axis of reference, and θ is illustrated in the cross section of described cylinder the angle position with respect to the magnet of the dipole axis of reference); Like this, this system produces magnetic dipole.Therefore produce magnetic field relatively uniformly, be that it has very little magnetic gradient, when attempting fast and effectively during separating magnetic particles, have implicit shortcoming (because, as mentioned above, big magnetic field gradient can increase the power that acts on the particle, and can increase the speed of described particle localization in the desired region of sample or acceptor).
U.S. Patent Application Publication No. US2003/0015474A discloses another kind of separator, it is equally based on cylinder, this cylinder is formed by 8 magnets, and wherein on the cross section of cylinder, each magnet has two sides and adjoins or the respective side of adjacent magnet is parallel and mutually by placing.The angular progression that the direction of magnetization of magnet is followed Δ γ=3 Δ θ (wherein, the magnetization angle direction that Δ γ is illustrated in the cross section of described cylinder between a magnet and the adjacent magnet changes, and Δ θ is illustrated in the cross section of described cylinder the variation of the angle position between a magnet and the adjacent magnet) (perhaps, describe in another way, follow the angular progression of γ=3 θ, here γ is illustrated in the cross section of described cylinder the magnetization angle direction with respect to the magnet of the dipole axis of reference, and θ is illustrated in the cross section of described cylinder the angle position with respect to the magnet of the dipole axis of reference); Therefore, this system produces the magnetic quadrapole.
Magnetic particle separator based on disclosed structure among the US5705064A produces strong magnetic field, and produces almost constant magnetic field gradient based on the separator of disclosed structure among the US2003/0015474A.These structures are based on the Halbach theorem, this theorem shows, if the magnetization perpendicular to its unlimited linear magnet that is magnetized centers on this rotation, the pattern of magnetic field in whole space is constant, and its direction is rotated along the direction opposite with direction of rotation with identical angle in whole space.Utilize this principle, can develop at the inner dipole source of uniform field that produces of cylinder chamber (for example, referring to H.A.Leupold, " Static Applications " in " Rare Earth Permanent Magnets ", J.M.D.Coey (Editor), 1996, pages 401-405).In addition, can realize that it has the advantage of secure context near zero magnetic field in the outside of cylinder.These structures are also referred to as " Halbach cylinder ".This principle can easily be used on the multipole Source, under the situation of quadrupole source, can realize constant gradient.
Usually, the magnetic particle separator is used for separating the magnetic particle of small size, normally 50ml or littler volume.But the technology of separating magnetic particles has important use equally, and wherein for technology and/or commercial object, it can be used to handle big volume (for example sample, solution, suspension), for example, and the volume of several upgradings.The volume of handling may change.Therefore, can easily expand if produce the structure of the system in magnetic field, this is favourable so.
Disclosed structure is based on the Halbach cylinder that magnet arranged side by side is formed among US5705064A and the US2003/0015474A, thus the side of each magnet with adjoin or the lateral magnets of adjacent magnets is parallel and mutually by placement.In the accompanying drawing of these two pieces of documents, be how to utilize magnet to realize this structure as can be seen, wherein in the cross section of this structure in the magnetic field that produces separator, the geometric configuration of this magnet (geometric configuration) is for having roughly trapezoidal by the less inboard and bigger outside of two engage sides, these two sides are corresponding to the side of this magnet, its with the side of magnet and adjacent magnets mutually by placing.Like this, this structure that produces magnetic field has inner surface and outer surface, and the cross section of this inner surface be the regular polygon that is formed by minor face, the serve as reasons regular polygon of long limit formation of the cross section of this outer surface.
Though these structures are the good great technical problem that do not exist in theory, (being applied to the acceptor of several ml level volumes) is no problem when being the system when the magnetic particle that separates small size at least, but can prove existing problems aspect extensibility and manufacture component.
For example, if attempt to increase the diameter of the inboard free space of cylinder, promptly increase the diameter in the space of placing the object (sample, suspension, solution, acceptor etc.) that stands the magnetic particle separating treatment, therefore described object must be exposed to magnetic field, and the size that must change magnet is can keep above-mentioned project organization.In other words, the magnet that is used in the separator that inside has definite diameter free space can not be used in the structure in another internal freedom space, at least be when the Halbach cylindrical structure is kept in hope can not, disclosed as US5705064A and US2003/0015474A.In addition, when increasing magnet size, because the increase of repulsive force between magnet, become more and more difficult such as the location of the magnet in those structures of describing among US5705064A and the US2003/0015474A.
On the other hand, can find that it is (in the cross section of this structure as seen) that changes that the geometric configuration of the magnet in the cross section of this structure and the pass between the direction of magnetization tie up between different magnets.For example, in US2003/0015474A, have at least three types relation between the magnetization of magnet and the geometric configuration:
-in two magnets, the direction of magnetization or orientation (S → N) point to less limit (inboard) from bigger limit (outside);
-in two magnets, the direction of magnetization (S → N) point to bigger limit (outside) from less limit (inboard);
-in four magnets, the direction of magnetization (S → N) be roughly parallel to bigger and less limit (from the outside, two in them is from left to right, and in addition two with opposite direction).
This means, when setting up a kind of structure as US2003/0015474A, must utilize at least three kinds of dissimilar magnets.The element of supposing the magnetic material that is used for this type of magnet has the preferred or easy direction of magnetization (corresponding to " easy magnetizing axis " of magnetic material), will obtain these three kinds of dissimilar magnets so and may process original magnetic material based on three kinds of different templates.In logic, may make that like this to obtain this structure more complicated and more expensive, under the situation of production small lot separator, especially be a problem, and may be more general when hope is the separator of particular customer and/or demands of applications and particular design.
Summary of the invention
For this reason, consider such fact, wish to set up the magnetic particle separator based on a kind of structure, this structure has extensibility, and more particularly, it can be used for some magnetic part of determining or magnet producing the structure in the magnetic field of different size.
One object of the present invention relates to a kind of device of separating magnetic particles, and this device comprises the non-uniform magnetic-field generator with cross section, and described cross section has the inner space that is used to place the object that will stand the magnetic particle separating treatment.
This generator comprises the supporting construction that is used for magnet and is positioned at a plurality of magnets of described supporting construction.In the cross section of the generator in comprising the plane of a plurality of described magnets, this magnet is polygonal structure with a plurality of limits (magnet equally may be for oval, round-shaped etc., because, for example circular polygon that can think to have countless limits).A plurality of magnets distribute angledly, form at least one magnet ring around the inner space, have the magnetic field that number of magnetic poles is P thereby produce in described inner space, and P is the even number greater than 2.
Each magnet has the direction of magnetization at the cross section of described generator, the magnet of described at least one ring is set so that the direction of magnetization of magnet is followed the angular progression of Δ γ=((P/2)+1) * Δ θ, wherein, Δ γ is illustrated in the cross section of described generator the variation of the direction of magnetization between a magnet and the adjacent magnet, and Δ θ is illustrated in the cross section of described generator the variation (and P is aforesaid number of magnetic poles) of the angle position between a magnet and the adjacent magnet.
Described at least one ring comprises that (that is, it has a large amount of magnets of Duoing than the number of magnetic poles in the magnetic field more than P magnet; Like this, can realize that entire inner space has the big and constant magnetic field of magnetic gradient, well-known, increase along with the magnet number, (for example, if only utilize 4 magnets, the place of close magnet can produce gradient " distortion " twisting minimizing near the distribution of the field in the zone of field source; But if utilize a large amount of magnets, then gradient almost is perfectly, does not promptly have big distortion, except very near in the zone of magnet surface).
According to this purpose of the present invention, in the cross section of generator, has N type magnet.In the cross section of generator, every kind of magnet all has definite geometric configuration, and has definite relation between its direction of magnetization and the described geometric configuration.According to this purpose of the present invention, N=1 or N=2.
This is favourable, because use a kind of or two kinds of magnets at the most, wherein every kind of magnet all has its oneself geometric configuration and magnetization/geometric configuration relation, owing to have the magnet kind number (1 or 2) of minimizing, make it have sizable flexibility, has advantage in logic, particular importance when it relates to manufacturing small lot separator.The present invention only uses one or both magnets just can make up the separator of different size and characteristic.This means that for example, magnet is depended in the production of separator, this magnet can by utilize a kind of or at the most two kinds of different templates a kind of magnetic material cutting is obtained (considering the preferred magnetization direction of this material).
Generator can be constructed by this way, in the cross section of described generator, magnet a plurality of limits in do not have with in described ring, be positioned at them angularly before or after the limit of magnet mutually by the limit of placing (still, each magnet can contact with each other and their face is formed by the magnet block of placement each other mutually by several).This distribution of magnet structurally has very big flexibility, and it can utilize identical magnet formation to have the structure of different size, and does not change the shape or the size of magnet, and uses the magnet of simple geometry configuration.According to this form of the present invention, the magnet that forms described ring for example can not contact each other, perhaps can contact other magnets in this ring, but contact point is only being located (leaning on mutually with the angle or the limit of another magnet) corresponding to the angle between the both sides of at least one described magnet.
Another object of the present invention relates to a kind of device of separating magnetic particles, and this device comprises the non-uniform magnetic-field generator with cross section, and described cross section has the inner space that is used to place the object that will stand the magnetic particle separating treatment.
Generator comprises the supporting construction that is used for magnet and is positioned at a plurality of magnets of described supporting construction, in the cross section of the generator in comprising the plane of a plurality of described magnets, the polygonal structure that described magnet has a plurality of limits (is included as the possibility of ellipse, circle etc., because, for example circular polygon that can think to have countless limits etc.).
A plurality of magnets distribute angledly, form at least one magnet ring around the inner space, have the magnetic field that number of magnetic poles is P so that produce in described inner space, and P is the even number greater than 2.
Each magnet has the direction of magnetization at the cross section of described separator, magnet to described at least one ring is provided with, so that the angular progression of Δ γ=((P/2)+1) * Δ θ is followed in the direction of magnetization of magnet or orientation, wherein Δ γ is illustrated in the cross section of described generator the variation of the direction of magnetization between a magnet and the adjacent magnet, and Δ θ is illustrated in the cross section of described generator the variation of the angle position between a magnet and the adjacent magnet, and described at least one ring has, and (that is, it has a large amount of magnets of Duoing than the field pole number that produces more than P magnet; Like this, can realize that entire inner space has the big and constant magnetic field of magnetic gradient, well-known, along with the increase of magnet number, twisting minimizing near the distribution of the field in the zone of field source; For example,, have sizable " distortion " near the magnetic gradient of magnet, yet if use a large amount of magnets, gradient there is not this big distortion basically, except unusual zone) near magnet surface if having only 4 magnets.
According to this purpose of the present invention, generator is constructed, so that in the cross section of described generator, in a plurality of limits of magnet the limit do not have with in described ring, be positioned at them angularly before or after the limit (though each magnet can be made of by the magnet block of placement each other mutually several faces) by placing mutually, limit of magnet.
This being configured in has bigger flexibility when designing magnet structure, it can utilize the magnet formation of single kind to have the structure (perhaps reducing number of magnets at least) of different size.In view of magnet do not contact each other or at least their face be not mutually by placing each other, can realize different magnet configurations, and not spend shape or the size that changes magnet or change the geometric configuration direction of magnetization with respect to magnet.
The magnet that forms ring can for example not contact each other, if perhaps have some contacts between two of described ring continuous angled magnets, this contact can be only corresponding to the angle between the both sides of at least one described magnet (leaning on the angle or the limit of another magnet) mutually.
In the cross section of described generator, for example, N kind magnet can be arranged, every kind of magnet has definite geometric configuration and definite its direction of magnetization and the relation between the described geometric configuration, at the cross section of described generator, N=1 or N=2.Use a kind of or two kinds of magnets at the most, every kind of magnet all has its geometric configuration and magnetization/geometric configuration relation, and the minimizing of magnet type number, makes it have quite big flexibility, from seeing that in logic this is favourable,, it is even more important when making the small lot separator that is used for specific purposes when relating to; The present invention can only use one or both magnets, and therefore can make up the separator of many different sizes and characteristic, and this makes and can obtain all magnets based on one or both template cutting magnetic materials.
In above-mentioned two purposes of the present invention, each can both be according to implemented in many forms.For example, in cross section, magnet can have essentially rectangular or hexagonal polygonal structure.
Another object of the present invention relates to a kind of device of separating magnetic particles, this device comprises the non-uniform magnetic-field generator, this generator comprises the cross section with inner space, this inner space is used to place the object that will stand the magnetic particle separating treatment, and described generator comprises the supporting construction that is used for magnet and is positioned at a plurality of magnets of described supporting construction.In the cross section of this generator in comprising the plane of a plurality of described magnets, described magnet is the polygonal structure with a plurality of limits.Magnet distributes angledly, forms at least one magnet ring around the inner space, has the magnetic field that number of magnetic poles is P so that produce in described inner space, and P is the even number greater than 2.
According to this purpose of the present invention, polygonal structure is a hexagonal configuration.Hexagonal configuration is very favorable, because it allows to utilize between the direction of magnetization of magnet and the geometric configuration relation seldom to set up the structure that is easy to expand, has implicit advantage (referring to above-mentioned explanation).For example, these structures can be expanded at an easy rate by removing a magnet ring.Magnet contacts with each other, and the limit of magnet forms honeycomb or similar type by being provided with mutually with the adjacent magnets limit, can make up these extendible magnet structures equally or have the structure of easy increase inner space.
Each magnet has the direction of magnetization at the cross section of described separator, and the magnet of described at least one ring is positioned, so that the direction of magnetization of magnet is followed the angular progression of Δ γ=((P/2)+1) * Δ θ, wherein, Δ γ is illustrated in the variation of the direction of magnetization between the magnet of the magnet of cross section of described generator and adjacency, and Δ θ is illustrated in the magnet of cross section of described generator and the variation of the angle position between the adjacent magnet.
Described at least one ring can comprise more than P magnet (that is, the magnet number is more than the number of magnetic poles in the magnetic field).Like this, can realize that entire inner space has the magnetic field of constant magnetic gradient, particularly when P=4 (when P＞4, gradient is non-constant, for example, works as P=6, and gradient is linear to rise, and the center gradient is zero, and its implicit separative efficiency is low).A large amount of magnets that utilization is Duoed than the number of magnetic poles in the magnetic field, can obtain the big and substantially invariable magnetic field of magnetic gradient in entire inner space, as everyone knows, increase along with the magnet number, (for example twisting minimizing near the distribution of the field in the zone of field source, if only utilize 4 magnets, then can produce gradient " distortion " near the magnet place; But if utilize a large amount of magnets, then gradient almost is perfectly-promptly not distortion basically, except very the zone near magnet surface).
In the cross section of described generator, N kind magnet can be arranged, every kind of magnet has definite geometric configuration and definite its direction of magnetization and the relation between the described geometric configuration, and at the cross section of described generator, for example, N may be 1 or 2.Use a kind of or two kinds of magnets at the most, every kind of magnet all has its geometric configuration and magnetization/geometric configuration relation, and the minimizing of magnet kind, allows to have sizable flexibility, from this is very favourable in logic, when relating to manufacturing small lot separator, it is even more important; The present invention allows only to use one or both magnets, and therefore can make up the separator of many different sizes and characteristic.
Generator can be provided with like this, in the cross section of described generator, do not have in a plurality of limits of magnet with in described ring, be positioned at them angularly before or after the limit (though each magnet can be made of by the placement magnet block each other mutually several faces) by placing mutually, limit of magnet.So structurally have big flexibility, it can utilize the magnet of same magnet or type to form the structure of different size, and does not change the shape or the size of magnet.According to this form of the present invention, can select the arrangement of magnet, so that forming the magnet of described ring does not contact each other, perhaps some magnet or all magnet contacts, but just contact by this way, be contact between the angled continuous magnet of in the described ring two corresponding to the angle between the both sides of at least one described magnet, lean on mutually with the angle or the limit of another magnet.
According to different forms, can be to arbitrary purpose structure of foregoing invention, it can comprise following optional feature some or all:
In cross section, the magnet of forming magnet ring has the direction of the geometric configuration of the angular progression of following Δ γ=((P/2)+1) * Δ θ, wherein Δ γ is illustrated in the cross section of described generator, the variation of the angle direction of the geometric configuration between magnet and the adjacent magnets, and Δ θ is illustrated in the magnet of cross section of described generator and the variation of the angle position between the adjacent magnets.In other words, magnet distributes like this, so that can change the angle direction of the geometric configuration of magnet, and need not change the magnetization with respect to described geometric configuration.This is favourable, because it allows to utilize single template cutting original magnetic material, promptly makes magnet block, and all magnet block have identical magnetization and the relation between the geometric configuration.
The number of magnetic poles P of entire inner space can be 4, has big in the magnetic field that obtains like this and constant gradient.
In the cross section of the described generator of magnet in comprising the described plane of a plurality of described magnets, magnet can have equilateral polygonal structure.
Magnet can be a parallelepiped.
In cross section, magnet can be distributed in the structure that comprises a plurality of concentric magnet rings.
This structure can comprise a plurality of magnet rings that distribute along the longitudinal axis that is approximately perpendicular to described cross section of device.
One or more magnets can be made up of at least two juxtaposed magnet block.
Supporting construction can comprise a plurality of support components of placing successively along the longitudinal axis of this device (for example aluminium loop type), and each support component has a plurality of holes that are used to place magnet, and the geometric configuration in this hole and the geometric configuration of magnet are complementary.
Magnet can be by for example NdFeB, SmCo, Ni makes, and perhaps more generally, magnet can be the magnet with magnetic anisotropy, for example, magnet with magnetocrystalline anisotropy (and does not have this characteristic, the risk of magnet degaussing can occur causing owing to the magnetic field that adjacent magnet produces, for example, if material is steel or AlNiCo, just may take place).
Another object of the present invention relates to the method for the magnetic particle in a kind of separating objects (for example, comprise the container of fluid, fluid for example is the liquid that has magnetic particle in the suspension).According to this purpose of the present invention, this method comprises object is placed on step according to the inner space of the device of above-mentioned arbitrary method.
Description of drawings
In order to remark additionally book and better understand feature of the present invention, according to the preferred example of practical embodiments of the present invention, provide one group of accompanying drawing, as the part of the whole of described specification, these accompanying drawings are expressed as follows with illustrative and nonrestrictive mode:
Shown in Fig. 1 is perspective illustration according to the supporting construction of the separator of possible embodiments of the present invention.
Shown in Fig. 2 is cross-sectional view according to the separator of possible embodiments of the present invention.
Shown in Fig. 3 and 4 is according to the location of the lateral magnets of the separator of two interchangeable embodiment of the present invention and the schematic diagram of their direction of magnetization.
Shown in Fig. 5 and 6 is supporting construction perspective views according to two of possible embodiments of the present invention assembling stages.
Fig. 7-9 shows the schematic diagram that is provided with based on the magnet structure in the separator sectional view among three embodiment of hexagon magnet.
Shown in Figure 10 is perspective view according to the complete separator of possible embodiments of the present invention.
The specific embodiment
Fig. 1 has illustrated the preferred embodiment that the present invention is feasible, and more particularly, supporting construction 2 is placed on the top of bearing or pedestal 24, and this supporting construction 2 comprises a plurality of support rings, and for example the aluminium ring is represented as ring 21,22,23 among the figure.Free space 1 in the ring is used to place will be by the sample of magnetic particle separating treatment or object.
As shown in the ring 21 (it has and other ring 22 and 23 identical or roughly the same structures), support ring has a series of hole or passage 2B that are provided with magnet in it, so although have between magnet and attract or repulsive force, magnet is maintained fixed motionless.Illustrated structure can also have the lid (not shown), is used to stop vertical the moving (the moving of the longitudinal axis that promptly is parallel to supporting construction) of magnet.Among Fig. 1, can see hole 2A equally, be used for bar is arranged in it with the connection of retaining ring that this bar can be made by brass or stainless steel.Described bar and aluminium ring 21,22,23, pedestal 24 and lid (not shown) form supporting construction together basically.
Magnet is arranged among passage or the hole 2B.Each magnet may be made up of two or polylith magnet, and these two or polylith magnet are juxtaposed so that form a magnet, and its cross section is corresponding to the cross section of hole or passage 2B, so magnet is can not move or quite limited mobile remaining in the described hole.
Fig. 2 has illustrated in this class supporting construction 2 that Fig. 1 represents, utilizes a plurality of brass or similar bar 25 to fix, and this bar passes the support ring of this structure, and a plurality of magnets 3 are placed in the 2B of hole, and each magnet has a plurality of limits.Especially, Fig. 2 has shown the cross section of separator, and has shown that at described cross section, magnet 3 has the polygon cross section, is rectangle in particular, perhaps more particularly is square.Do not contact each other between the magnet.Especially, the limit of magnet or face 3a, 3b, 3c and 3d not with the face of adjacent magnets or limit mutually by placing (though the angle that might magnet and the angle or the edge joint of adjacent magnets touch, it does not exceed scope of the present invention yet).As can be known from Fig. 2, a plurality of magnets 3 are set up and form magnet ring 4, needn't make limit between magnet each other mutually by placing, mean the magnet of ring on 4 and the variation of the direction of magnetization between adjacent magnets, can set up by changing physical piece and the relation between the supporting construction of forming magnet, not need to use the magnet block that between the direction of magnetization (direction of magnetization in the cross section of separator) and geometric configuration, has different relations.
Can more easily understand this principle referring to Fig. 3, Fig. 3 shows the distribution of the magnet 3 in the separator cross section in possible embodiments of the present invention.As shown in the figure, for all magnets, the arrow 5 in the indication direction of magnetization or orientation has identical relation with respect to the geometric configuration of the magnet in the separator cross sectional planes.
Especially, all magnets all have and are parallel to its both sides and perpendicular to the other direction of magnetization on both sides.This means that along the direction of easy axis that is parallel and perpendicular to described material the direction of the what is called " easy magnetizing axis " of material (promptly corresponding to), all magnets can both obtain based on magnetic material of same template cutting.
As shown in Figure 3, wherein shown the distribution that produces magnet in the inner space of separator with quadripolar magnetic field, the direction of magnetization 5 of the magnet 3 of magnet ring 4 is followed the angular progression of γ=3* Δ θ, wherein, Δ γ is illustrated in the cross section of described separator the variation of the direction of magnetization 5 between a magnet 3 and the adjacent magnet, and Δ θ is illustrated in the cross section of described separator the variation of the angle position between a magnet 3 and the adjacent magnet.Yet, according to the present invention, be not to realize with respect to the relation between the geometric configuration of magnet, but magnet realizes with respect to the geometric configuration direction of supporting construction by changing by the direction of magnetization that changes magnet; Especially, as shown in Figure 3, the a plurality of magnets 3 that form magnet ring 4 have the geometric configuration direction of the angular progression of following Δ γ=3 Δ θ, wherein, Δ γ is illustrated in the variation of the angle direction of the magnet 3 of cross section of described separator and the geometric configuration between the adjacent magnet, and Δ θ is illustrated in the magnet of cross section of described separator and the variation of the angle position between the adjacent magnet.In other words because the limit of magnet not with the limit of adjacent magnet mutually by placing, produce the angular progression of the direction of magnetization so the respective angles of the direction of physical unit that can be by forming magnet is gone forward one by one.
In being similar to shown in Figure 3 the setting, the inductive mode of the magnetic field of generation (B) radially increases; It is responded to from the height that zero induction that encircles 4 centers (center in internal freedom space 1) changes to edge (near magnet ring), has the gradient of constant, and typically, this gradient can be several T/m.This constant gradient causes being placed on the magnetic particle that is occurred in the sample of inner space, moves to chamber wall on the cross section of separator at least, and for example, described sample is placed in the container that occupies most of described inner space.Among Fig. 3, be the direction that arrow in " inner space " 1 of profile is represented magnetic gradient, also be on the magnetic particle that is applied in the sample therefore and make the direction of the power that they move to the wall of a container that comprises this sample to encircle 4.Among Fig. 3, the line of sub-circular is represented equipotential line, i.e. the line that is formed by the point of the magnetic field intensity with identical value (this is equally applicable to illustrate other figure of such line and arrow).
Fig. 4 shows the distribution according to another possible embodiments magnet of the present invention.In this embodiment, magnet 3 distributes along two rings; The angular progression of their direction of magnetization 5 is identical with being provided with shown in Fig. 3, but in this case, utilizes two magnet rings, one has 22 magnets, and another promptly the outside be 30 magnets, and magnet type is identical with type during Fig. 3 is provided with, the magnetic field of having realized bigger gradient.
According to feasible preferred embodiment of the present invention, shown in Fig. 5 be the assembling in supporting construction.Especially, three rings the 21,22, the 23rd have been shown, how to be fixed on the substrate 24 that described ring for example is the aluminium ring with about 10mm height.Described ring can utilize laser cutting to make by the thick aluminium sheet of for example 10mm.
Above-mentioned ring interfixes by fixed system, and this fixed system comprises bar 25, and this bar for example is brass or nonmagnetic stainless steel.Bar 25 is threaded, and utilizes bolt 26 that the aluminium ring is fixed on desirable height, and this bolt for example is a plastic bolt.Illustrated also among the figure each magnet 3 is how to be made up of two parts 31,32, and these two parts constitute magnet 3 together.
Fig. 6 shows another assembling stage of separator, wherein, has increased another aluminium ring 20, and has comprised all magnet 3, and each magnet all is made up of two parts 31,32.Structure shown in Fig. 6 has three layers of magnet.Magnet can be the magnet of NdFeB magnet or any other suitable material for example, and this depends on that expection wants the concrete property that obtains.
Fig. 7 has illustrated another feasible embodiment of the present invention, and wherein used magnet 3 has hexagonal cross-section, and is placed in the ring of inner space 1, and this inner space is used to place processed sample or object.In this structure, utilized magnet with hexagonal cross-section, when keep to the side to place in the magnet limit (both sides of same magnet and the corresponding edge of adjacent magnets are mutually by placing), has single relation between the geometric configuration of the cross section of the direction of magnetization and magnet, can realize the suitable angular progression of the direction of magnetization 5, have the advantage of implicit configuration aspects.
Fig. 8 shows another setting based on two hexagon magnet rings, and two magnet rings encircle and outer shroud promptly, and all magnets have such side, and this side leans against the side of the adjacent magnets of same and another magnet ring.In this case, all magnets have identical geometric configuration, but have two kinds of relations between magnetization and the geometric configuration: as shown in the figure, some magnet 3A have the direction of magnetization 5 perpendicular to two faces of magnet, and other magnets 3B has the direction at the edge between two faces.
Fig. 9 shows the another kind setting based on the hexagonal cross-section magnet; Inner space 1 has shown direction (having arrow) and some equipotential lines of magnetic gradient, and equipotential line is promptly formed by the point of the magnetic field cross sectional strength with identical value.
From these figure, be easy to find out, this " honeycomb " shape of different hexagon magnets " ring " is provided with very important advantage, because it can easily be designed to extendible system: for example, for the diameter of the inner space 1 of the separator that increases structure shown in Figure 8,6 magnets in can easily removing in the ring etc.
In Figure 10, shown complete separator, it is based on the design shown in Fig. 5 and 6, but has shell 29 and lid 27; Lid utilizes screw 28 to be fixed on the bar 25 (not shown among Figure 10).
In this article, word " comprises " and is not out of shape and should be understood as that eliminating, that is to say, they do not get rid of the possibility of parts that described term comprises other, step etc.
In addition, in the scope that claim is inferred, the invention is not restricted to above-described specific embodiment, and cover the variation that those skilled in the art for example may the make selection of (for example about material, size, component, structure or the like) equally.
Claims (39)
1. the device of a separating magnetic particles comprises:
Non-uniform magnetic-field generator with cross section, described cross section have the inner space (1) that is used to place the object that will stand the magnetic particle separating treatment,
Described generator comprises supporting construction (2) and a plurality of magnet (3) that is positioned at described supporting construction (2) that is used for support magnet,
In the cross section of the described generator in comprising the plane of described a plurality of magnets, described magnet (3) is the polygonal structure with a plurality of limits,
Described a plurality of magnet (3) distributes angledly, forms at least one magnet ring (4) around described inner space, has the magnetic field that number of magnetic poles is P so that produce in described inner space (1), and P is the even number greater than 2,
Each magnet, (3) in the cross section of described generator, has the direction of magnetization, (5), described at least one ring, (4) a plurality of magnets, (3) be positioned, so that the direction of magnetization of described magnet, (5) follow Δ γ=, (, (P/2)+1) angular progression of * Δ θ, wherein, Δ γ is illustrated in the cross section of described generator the variation of the direction of magnetization between a magnet and the adjacent magnet, and Δ θ is illustrated in the cross section of described generator the variation of the angle position between a magnet and the adjacent magnet, and described at least one ring comprises more than P magnet
It is characterized in that:
In the cross section of described generator, described a plurality of magnets have N type, every type magnet all have definite geometric configuration with and the direction of magnetization and described geometric configuration between definite relation, in described cross section, N=1 or N=2.
2. device according to claim 1 is characterized in that: described generator is configured, so that in the cross section of described generator, described magnet (3a, 3b, 3c, do not have in a plurality of limits 3d) with described ring in be positioned at the magnet before or after them angularly the limit by placing mutually, limit.
3. device according to claim 2 is characterized in that: the described magnet (3) that forms described ring does not contact each other.
4. device according to claim 2 is characterized in that: if having contact between the magnet (3) that two are provided with angularly continuously in the described ring, then described contact is only corresponding to an angle between the both sides of at least one described magnet.
5. device according to claim 1 is characterized in that: number of magnetic poles P=4.
6. device according to claim 1 is characterized in that: in the cross section of described generator, comprise that the magnet (3) in the described plane of a plurality of described magnets has the equilateral polygon structure.
7. device according to claim 1 is characterized in that: described magnet is the parallelepiped structure.
8. device according to claim 1 is characterized in that: in described cross section, described magnet distributes with the structure that comprises a plurality of concentric magnet rings.
9. device according to claim 1 is characterized in that: described generator comprises a plurality of magnet rings that distribute along the longitudinal axis that is approximately perpendicular to described cross section of described device.
10. device according to claim 1 is characterized in that: at least one described magnet is made up of at least two juxtaposed magnet block.
11. device according to claim 1, it is characterized in that: described supporting construction (2) comprises a plurality of support components (21 of placing successively along the longitudinal axis of described device, 22,23), each support component all has a plurality of holes (2B) that are used to place described magnet, and the geometric configuration of the geometric configuration in described hole and described magnet (3) is complementary.
12. the device of a separating magnetic particles comprises:
Non-uniform magnetic-field generator with cross section, described cross section have the inner space (1) that placement will stand the object of magnetic particle separating treatment,
Described generator comprises supporting construction (2) and a plurality of magnet (3) that is positioned at described supporting construction (2) that is used for support magnet,
In the cross section of the described generator in comprising the plane of a plurality of described magnets, described magnet (3) is the polygonal structure with a plurality of limits,
Described a plurality of magnet (3) distributes angledly, forms at least one magnet ring (4) around described inner space, has the magnetic field that number of magnetic poles is P so that produce in described inner space (1), and P is the even number greater than 2,
Each magnet, (3) cross section at described generator has the direction of magnetization, (5), described at least one ring, (4) a plurality of magnets, (3) be positioned, so that the direction of magnetization of described magnet, (5) follow Δ γ=, (, (P/2)+1) angular progression of * Δ θ, wherein Δ γ is illustrated in the cross section of described generator the variation of the direction of magnetization between a magnet and the adjacent magnet, and Δ θ is illustrated in the cross section of described generator the variation of the angle position between a magnet and the adjacent magnet, and described at least one ring comprises more than P magnet
It is characterized in that:
Described generator is configured so that in the cross section of described generator, described magnet (3a, 3b, 3c, do not have in a plurality of limits 3d) with described ring in be positioned at the magnet before or after them angularly the limit by placing mutually, limit.
13. device according to claim 12 is characterized in that: the described magnet (3) that forms described ring does not contact each other.
14. device according to claim 12 is characterized in that: if having contact between two continuous angularly magnets (3) in the described ring, then described contact is only corresponding to an angle between the both sides of at least one described magnet.
15. according to the described device of each claim among the claim 12-14, it is characterized in that: in described cross section, described magnet has the polygonal structure of rectangle.
16. according to the described device of each claim among the claim 12-14, it is characterized in that: in described cross section, described magnet has hexagonal polygonal structure.
17. device according to claim 12 is characterized in that: number of magnetic poles P=4.
18. device according to claim 12 is characterized in that: in the cross section of described generator, comprise that the magnet (3) in the described plane of a plurality of described magnets has the equilateral polygon structure.
19. device according to claim 12 is characterized in that: described magnet is the parallelepiped structure.
20. device according to claim 12 is characterized in that: in described cross section, described magnet distributes with the structure that comprises a plurality of concentric magnet rings.
21. device according to claim 12 is characterized in that: described generator comprises a plurality of magnet rings that distribute along the longitudinal axis that is approximately perpendicular to described cross section of described device.
22. device according to claim 12 is characterized in that: at least one described magnet is made up of at least two juxtaposed magnet block.
23. device according to claim 12, it is characterized in that: described supporting construction (2) comprises a plurality of support components (21 of placing successively along the longitudinal axis of described device, 22,23), each support component all has a plurality of holes (2B) that are used to place described magnet, and the geometric configuration of the geometric configuration in described hole and described magnet (3) is complementary.
24. the device of a separating magnetic particles comprises:
Non-uniform magnetic-field generator with cross section, described cross section have the inner space (1) that placement will stand the object of magnetic particle separating treatment,
Described generator comprises supporting construction (2) and a plurality of magnet (3) that is positioned in the described supporting construction (2) of support magnet,
In the cross section of the described generator on the plane that comprises a plurality of described magnets, described magnet (3) is the polygonal structure with a plurality of limits,
Described a plurality of magnet (3) distributes angledly, forms at least one magnet ring (4) around described inner space, has the magnetic field that number of magnetic poles is P so that produce in described inner space (1), and P is the even number greater than 2,
It is characterized in that: described polygonal structure is a hexagonal configuration.
25. device according to claim 24, it is characterized in that: each magnet (3) has the direction of magnetization (5) in the cross section of described generator, and a plurality of magnets (3) of described at least one ring (4) are positioned, so that the direction of magnetization of described magnet (5) is followed the angular progression of Δ γ=((P/2)+1) * Δ θ, wherein Δ γ is illustrated in the cross section of described generator the variation of the direction of magnetization between a magnet and the adjacent magnet, and Δ θ is illustrated in the cross section of described generator the variation of the angle position between a magnet and the adjacent magnet.
26. according to claim 24 or 25 described devices, it is characterized in that: described at least one ring (4) comprises more than P magnet.
27. according to claim 24 or 25 described devices, it is characterized in that: in the cross section of described generator, described a plurality of magnet has N type, every type magnet all have definite geometric configuration with and the direction of magnetization and described geometric configuration between definite relation, in described cross section, N=1 or N=2.
28. according to claim 24 or 25 described devices, it is characterized in that: described generator is configured, so that in the cross section of described generator, described magnet (3a, 3b, 3c, do not have in a plurality of limits 3d) with described ring in be positioned at the magnet before or after them angularly the limit by placing mutually, limit.
29. device according to claim 28 is characterized in that: the described magnet (3) that forms described ring does not contact each other.
30. device according to claim 28 is characterized in that: if having contact between the magnet (3) that two are provided with angularly continuously in the described ring, then described contact is only corresponding to an angle between the both sides of at least one described magnet.
31. device according to claim 24, it is characterized in that: in described cross section, the described magnet (3) of forming magnet ring has the direction of the geometric configuration of the angular progression of following Δ γ=((P/2)+1) * Δ θ, wherein Δ γ is illustrated in the cross section of described generator the variation of the angle direction of the described geometric configuration between a magnet and the adjacent magnet, and Δ θ is illustrated in the cross section of described generator the variation of the angle position between a magnet and the adjacent magnet.
32. device according to claim 24 is characterized in that: number of magnetic poles P=4.
33. device according to claim 24 is characterized in that: in the cross section of described generator, comprise that the magnet (3) in the described plane of a plurality of described magnets has the equilateral polygon structure.
34. device according to claim 24 is characterized in that: described magnet is the parallelepiped structure.
35. device according to claim 24 is characterized in that: in described cross section, described magnet distributes with the structure that comprises a plurality of concentric magnet rings.
36. device according to claim 24 is characterized in that: described generator comprises a plurality of magnet rings that distribute along the longitudinal axis that is approximately perpendicular to described cross section of described device.
37. device according to claim 24 is characterized in that: at least one described magnet is made up of at least two juxtaposed magnet block.
38. device according to claim 24, it is characterized in that: described supporting construction (2) comprises a plurality of support components (21 of placing successively along the longitudinal axis of described device, 22,23), each support component all has a plurality of holes (2B) that are used to place described magnet, and the geometric configuration of the geometric configuration in described hole and described magnet (3) is complementary.
39. the method for the magnetic particle in the separating objects is characterized in that: it comprises the step of inner space of object being put into the described device of aforementioned each claim.
Applications Claiming Priority (3)
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| ESP200501550 | 2005-06-24 | ||
| PCT/EP2006/004363 WO2006136237A1 (en) | 2005-06-24 | 2006-05-10 | Device and method for separating magnetic particles |
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| CN101208153A CN101208153A (en) | 2008-06-25 |
| CN101208153B true CN101208153B (en) | 2010-09-22 |
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| JP (2) | JP5763875B2 (en) |
| CN (1) | CN101208153B (en) |
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| EP1904237B1 (en) | 2013-08-14 |
| WO2006136237A1 (en) | 2006-12-28 |
| JP5763875B2 (en) | 2015-08-12 |
| JP2014054633A (en) | 2014-03-27 |
| CN101208153A (en) | 2008-06-25 |
| EP1904237A1 (en) | 2008-04-02 |
| JP2008543548A (en) | 2008-12-04 |
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Effective date of registration: 20120821 Address after: E08290 Valley, Spain Patentee after: I MAG System Co. Ltd. Address before: E-08290 Valley, Spain Patentee before: Analisi Tecnologica Innovadora |