CN101190315A - Traditional Chinese medicine composition for treating melancholia and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating melancholia and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the Chinese traditional medicine field, in particular to a Chinese traditional medicine composition used for treating and fighting against depression, which is prepared by the raw materials with the following weight portions: 6-12 portions of Danshen root, 10-20 portions of lancelesf lily bulb, 10-20 portions of Chinese date, 4-8 portions of baked liquoric root, 20-40 portions of wheat and 8-16 potions of rehmanniae praeparatum. The Chinese traditional medicine composition of the invention has the function of treating depression as well as the function of treating both the symptoms and root causes, which can effectively cure depression. The invention also discloses the preparation method of the Chinese traditional medicine composition.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to the antidepressant Chinese medicine composition of a kind of treatment; In addition, the invention still further relates to the preparation method of this Chinese medicine composition.
Background technology
Depression is a kind of affective disorders that comprises the complexity of multiple mental symptom and somatization, mental state is low except that having in clinical manifestation, hebetude, retardation of thinking, self evaluation are crossed the mental symptom such as low, also comprise body not just as fatigue, insomnia, inappetence, hyposexuality, headache, dizziness, thirsty, pharyngeal discomfort, uncomfortable in chest, nervous, stomachache, abdominal distention or the like, severe patient can have from the fear sense, even suicidal tendency.
Along with the progress and the expanding economy of society, the aggravation of social competition, the quickening of rhythm of life, the rapid transition of social status, depression has become the commonly encountered diseases of modern society, high morbidity, and its sickness rate is soaring fast.It is reported that the homicide rate of China has reached 22.2/10 ten thousand populations, and wherein relevant with depression suicide accounts for about seventy percent.Because the various consumption of depressive disorder, the output value in whole world every year will lose 2%～5%, World Health Organization (WHO) predicts the year two thousand twenty, and depressive disorder will become the most serious disease burden of developing country, and severe depression can become dead and disabled second largest reason when the time comes.
The main at present employing Drug therapy of this disease, psychological transporting.In China based on Drug therapy.The clinical tricyclic antidepressants medicine depression that adopts is treated as imipramine, amitriptyline and the equality of worrying too much more, certain curative effect is arranged, but such medicine exists easily generation drug dependence and other side effect, uses and is subjected to certain limitation.In recent years along with people to the pathogenetic research that deepens continuously of depression, antidepressant drug has also had considerable progress, but the problem that depressed spectrum is narrow, relapse rate is high, side effect is big and the medicine valency is high thoroughly solution yet.
The traditional Chinese medical science adopts the dialectical thinking mode of logical reasoning to the diagnosis and treatment of disease, to show as the main depression of changing into focus with reactivity of organism with whole body the merit for the treatment of both the principal and secondary aspects of a disease can be arranged.Control according to differential diagnosis in tcm opinion, depressed card can be divided into following five types: liver qi stagnation type, stagnation of QI phlegm stagnancy type, melancholy overtax one's nerves type, type of deficiency of both the heart and spleen, type of hyperactivity of fire caused by deficiency of YIN.But antidepressant Chinese patent medicine in the market is few, and what have has also used comparatively expensive composition, does not reach gratifying effect.Therefore, still there is demand in people's Chinese medicine preparation that curative effect is better treated depression.
Summary of the invention
One of technical problem to be solved by this invention is to propose a kind of Chinese medicine composition that can effectively treat depression.
Two of technical problem to be solved by this invention is the preparation methoies that propose the Chinese medicine composition of above-mentioned treatment depression.
The present invention one of solves the problems of the technologies described above a kind of Chinese medicine composition for the treatment of depression that proposes, it is prepared from medicament by following bulk drugs, and this medicament is a said dosage form on any pharmaceutics: 6～12 parts of Radix Salviae Miltiorrhizaes, 10～20 parts of Bulbus Liliies, 10～20 parts in Fructus Jujubae, 4～8 parts of Radix Glycyrrhizae Preparatas, 20～40 parts of Huai wheats, 8～16 parts in the Radix Rehmanniae.
As the preferred technical solution of the present invention, above-mentioned pharmacy optimization is a granule, and the umber of each raw material is preferably: 9 parts of Radix Salviae Miltiorrhizaes, 15 parts of Bulbus Liliies, 15 parts in Fructus Jujubae, 6 parts of Radix Glycyrrhizae Preparatas, 30 parts of Huai wheats, 12 parts in the Radix Rehmanniae.
For solve the problems of the technologies described above two, the method for above-mentioned raw materials being made Chinese medicine composition of the present invention comprises the steps:
(a) take by weighing Radix Salviae Miltiorrhizae, Bulbus Lilii, Fructus Jujubae, Radix Glycyrrhizae Preparata, Huai wheat and the Radix Rehmanniae;
(b) decocting that adds 8 times of amounts boils twice, decocts 1 hour at every turn, merges decocting liquid twice, filters;
(c) filtrate being concentrated into relative density is 1.20, with 90%～95% ethanol precipitate with ethanol;
(d) reclaim ethanol and to be concentrated into relative density be 1.36, vacuum drying under 60 ℃～80 ℃ condition, dry extract, be used to prepare said dosage form on any pharmaceutics that comprises oral liquid and granule.Dry extract being pulverized, added dextrin and mix, is wetting agent with ethanol, granulate, and drying, granulate promptly makes granule.
With respect to prior art, Chinese medicine composition of the present invention selects to have resolving stagnation for tranquilization, Radix Salviae Miltiorrhizae, Bulbus Lilii, Fructus Jujubae, Radix Glycyrrhizae Preparata, Huai wheat and the Radix Rehmanniae of effect of relieving restlessness of clearing away heart-fire makes up, pharmacodynamics test and clinical observation on the therapeutic effect test prove that all Chinese medicine composition of the present invention all has antidepressant function, and have the merit for the treatment of both the principal and secondary aspects of a disease, can treat depression effectively.
The specific embodiment
Below in conjunction with embodiment the present invention is further described.
Used Radix Salviae Miltiorrhizae, Bulbus Lilii, Fructus Jujubae, Radix Glycyrrhizae Preparata, Huai wheat and the Radix Rehmanniae of the embodiment of the invention all meets the relevant every regulation under each medical material item of text of Chinese Pharmacopoeia version in 2005.Each medical material has passed through processed such as clean system, cutting, the process of preparing Chinese medicine, pulverizing, before feeding intake, is tested and appraised, and each medical material material object of distinguishing the flavor of conforms to title, and quality meets state-promulgated pharmacopoeia standard (concrete medical material evaluation way is carried out with reference to standards of pharmacopoeia).
Embodiment 1
Take by weighing Radix Salviae Miltiorrhizae 900 grams, Bulbus Lilii 1500 grams, Fructus Jujubae 1500 grams, Radix Glycyrrhizae Preparata 600 grams, Huai wheat 3000 grams, the Radix Rehmanniae 1200 grams;
The decocting that adds 8 times of amounts boils twice, decocts 1 hour at every turn, merges decocting liquid twice, filters;
It is 1.20 that filtrate is concentrated into relative density, with 93% ethanol precipitate with ethanol;
Reclaiming ethanol and being concentrated into relative density is 1.36, and vacuum drying under 70 ℃ condition gets dry extract;
Dry extract being pulverized, added an amount of dextrin and mix, is wetting agent with ethanol, granulate, and drying, granulate makes granule 1329.4 grams.
Embodiment 2
Take by weighing Radix Salviae Miltiorrhizae 600 grams, Bulbus Lilii 2000 grams, Fructus Jujubae 2000 grams, Radix Glycyrrhizae Preparata 600 grams, Huai wheat 3000 grams, the Radix Rehmanniae 1000 grams;
The decocting that adds 8 times of amounts boils twice, decocts 1 hour at every turn, merges decocting liquid twice, filters;
It is 1.20 that filtrate is concentrated into relative density, with 90% ethanol precipitate with ethanol;
Reclaiming ethanol and being concentrated into relative density is 1.36, and vacuum drying under 80 ℃ condition gets dry extract;
Dry extract being pulverized, added an amount of dextrin and mix, is wetting agent with ethanol, granulate, and drying, granulate makes granule 1364.3 grams.
Embodiment 3
Take by weighing Radix Salviae Miltiorrhizae 900 grams, Bulbus Lilii 1200 grams, Fructus Jujubae 1200 grams, Radix Glycyrrhizae Preparata 900 grams, Huai wheat 3500 grams, the Radix Rehmanniae 1200 grams;
The decocting that adds 8 times of amounts boils twice, decocts 1 hour at every turn, merges decocting liquid twice, filters;
It is 1.20 that filtrate is concentrated into relative density, with 95% ethanol precipitate with ethanol;
Reclaiming ethanol and being concentrated into relative density is 1.36, and vacuum drying under 60 ℃ condition gets dry extract;
Dry extract being pulverized, added an amount of dextrin and mix, is wetting agent with ethanol, granulate, and drying, granulate makes granule 1301.2 grams.
Embodiment 4
Take by weighing Radix Salviae Miltiorrhizae 1200 grams, Bulbus Lilii 2000 grams, Fructus Jujubae 1500 grams, Radix Glycyrrhizae Preparata 600 grams, Huai wheat 2500 grams, the Radix Rehmanniae 900 grams;
The decocting that adds 8 times of amounts boils twice, decocts 1 hour at every turn, merges decocting liquid twice, filters;
It is 1.20 that filtrate is concentrated into relative density, with 91% ethanol precipitate with ethanol;
Reclaiming ethanol and being concentrated into relative density is 1.36, and vacuum drying under 75 ℃ condition gets dry extract;
Dry extract being pulverized, added an amount of dextrin and mix, is wetting agent with ethanol, granulate, and drying, granulate makes granule 1261.0 grams.
Embodiment 5
Take by weighing Radix Salviae Miltiorrhizae 900 grams, Bulbus Lilii 1500 grams, Fructus Jujubae 1500 grams, Radix Glycyrrhizae Preparata 500 grams, Huai wheat 3000 grams, the Radix Rehmanniae 1500 grams;
The decocting that adds 8 times of amounts boils twice, decocts 1 hour at every turn, merges decocting liquid twice, filters;
It is 1.20 that filtrate is concentrated into relative density, with 93.5% ethanol precipitate with ethanol;
Reclaiming ethanol and being concentrated into relative density is 1.36, and vacuum drying under 65 ℃ condition gets dry extract;
Dry extract being pulverized, added an amount of dextrin and mix, is wetting agent with ethanol, granulate, and drying, granulate makes granule 1239.7 grams.
Below further set forth the beneficial effect of medicine of the present invention by testing example, these are tested examples and have comprised that the pharmacodynamics test of medicine of the present invention (to call the granule of soothing the spirit in the following text) and clinical observation on the therapeutic effect test.
Test example 1 (medicine of the present invention is to the influence of behavior in the mice forced swimming test)
(1) test material:
1. medicine
(prozac is in fluoxetine 20mg/ grain for fluoxetine hydrochloride capsules.Factory: Eli Lilly CompanyLimited.Batch number: A042238.Date of manufacture: 2003.09.11).
(per 15 balls weigh 1 gram to depression alleviating pills.Yumi Pharmaceutical Co., Ltd., Zhengzhou.Batch number: 20030602.Date of manufacture: 2003.06.25).
Soothing the spirit, (every gram granule is equivalent to crude drug 5.8 grams to granule.Self-control.Batch number: 20030519.Date of manufacture: 20030519).
2. experimental animal:
SPF level Male Kunming strain mice, body weight 18-22g before the experiment is provided by Chinese Academy of Sciences's Shanghai Experimental Animal Center.Room temperature is raised, free illumination and diet.
(2) test method:
With mice be divided into normal group at random, totally six groups of the administration groups of large, medium and small three dosage of the granule of soothing the spirit, Chinese medicine positive controls (depression alleviating pills), Western medicine positive controls (fluoxetine), 12 every group.Each treated animal is irritated stomach and is given relative medicine, and wherein normal group is given normal saline.
2 weeks of successive administration, after administration last day one hour with the single open glass cylinder of putting into of mice, observed 6 minutes, write down the swimming accumulative total dead time in back 4 minutes.
(3) result of the test:
Experimental result sees Table 1.Compare with normal group, big or middle dosage group of the granule of soothing the spirit and fluoxetine group have all shortened the mice non-swimming time, and difference is extremely remarkable.The depression alleviating pills group significantly shortens the mice non-swimming time, then there was no significant difference of the granule small dose group of soothing the spirit.
Table 1 medicine is to the influence of mice forced swimming test dead time
| Group | Dosage | Number of animals | Non-swimming time (s) |
| Normal group | 20mL/kg | 12 | 103.75±27.88 |
| The heavy dose of group of the granule of soothing the spirit | 3.9g/kg | 12 | 40.67±32.63** |
| The dosage group in the granule of soothing the spirit | 1.9g/kg | 12 | 65.58±37.14** |
| The granule small dose group of soothing the spirit | 1.0g/kg | 12 | 79.33±43.72 |
| Depression alleviating pills | 1.5g/kg | 12 | 74.25±30.44* |
| Fluoxetine | 2.5mg/kg | 12 | 45.00±33.95** |
Compare with normal group: * P＜0.05, * * P＜0.01.
Test example 2 (medicine of the present invention is not to predicting chronic stress and the lonely foster influence that combines rat model)
(1) test material:
1. medicine: (prozac is in fluoxetine 20mg/ grain for fluoxetine hydrochloride capsules.Factory: Eli LillyCompany Limited.Batch number: A042238.Date of manufacture: 2003.09.11).
(per 15 balls weigh 1 gram to depression alleviating pills.Yumi Pharmaceutical Co., Ltd., Zhengzhou.Batch number: 20030602.Date of manufacture: 2003.06.25).
Soothing the spirit, (every gram granule is equivalent to crude drug 5.8 grams to granule.Self-control.Batch number: 20030519.Date of manufacture: 20030519).
2. laboratory animal:
SPF level SD male rat, body weight 180-220g before the experiment is provided by Chinese Academy of Sciences's Shanghai Experimental Animal Center.Except that the modeling time, room temperature is raised, free illumination and diet.
(2) test method:
With rat be divided into normal group, model group at random, the administration group of large, medium and small three dosage of the granule of soothing the spirit, Chinese medicine positive controls (depression alleviating pills), Western medicine positive controls (fluoxetine), totally seven groups, 10 every group.Except that 5 in the every cage of normal rats, all the other each treated animals all give chronic stress and combine modeling, 1 in every cage with lonely supporting.Modeling is gastric infusion simultaneously, and wherein normal group and model group are given normal saline, and all the other each groups are given relative medicine, carry out official testing after continuous 3 weeks.
Adopt and do not predict chronic stress and the lonely foster depression model that combines.Normal rats will not any stimulation.
The behavior of observing animal with the Open-Field method changes.After administration last day one hour, adapt to one minute after animal put into the spacious case of cube, be determined at horizontal anomalous movement score and vertical activity score in 3 minutes then.
(3) experimental result
Experimental result sees Table 2.Model group and normal group compare, and the horizontal movement and time number average that moves both vertically obviously reduce, and difference is extremely remarkable; The horizontal movement of the heavy dose of group of granule of soothing the spirit and the horizontal movement of fluoxetine group, the number of times that moves both vertically compare with model group, and utmost point significant difference is all arranged; The horizontal movement of the moving both vertically of the heavy dose of group of the granule of soothing the spirit, middle dosage group and the horizontal movement of depression alleviating pills, move both vertically number of times and model group relatively have significant difference; The horizontal movement and the number of times and the model group comparison there was no significant difference of moving both vertically of the moving both vertically of the dosage group in the granule of soothing the spirit, small dose group.
Table 2 medicine combines the influence that rat model behavioristics measures to chronic stress with lonely supporting
| Group | Dosage | Number of animals | Horizontal movement (inferior) | (inferior) moves both vertically |
| Normal group | 20mL/kg | 8 | 52.12±15.83** | 23.63±12.73** |
| Model group | 20mL/kg | 8 | 21.62±14.28 | 8.13±6.73 |
| The heavy dose of group of the granule of soothing the spirit | 2.7g/kg | 8 | 49.25±18.70** | 21.00±13.03* |
| The dosage group in the granule of soothing the spirit | 1.3g/kg | 8 | 40.50±17.74* | 17.00±9.94 |
| The granule small dose group of soothing the spirit | 0.68g/kg | 8 | 30.37±14.12 | 14.37±7.54 |
| Depression alleviating pills | 1.1g/kg | 8 | 39.00±17.62* | 18.13±8.97* |
| Fluoxetine | 1.8mg/kg | 8 | 51.00±21.81** | 20.63±8.98** |
Compare with model group: * P＜0.05, * * P＜0.01.
Test example 3 (influence of the motionless state behavior that medicine of the present invention causes electricity irritation mice cornea)
(1) test material:
1. medicine:
(prozac is in fluoxetine 20mg/ grain for fluoxetine hydrochloride capsules.Factory: Eli Lilly CompanyLimited.Batch number: A042238.Date of manufacture: 2003.09.11).
(per 15 balls weigh 1 gram to depression alleviating pills.Yumi Pharmaceutical Co., Ltd., Zhengzhou.Batch number: 20030602.Date of manufacture: 2003.06.25).
Soothing the spirit, (every gram granule is equivalent to crude drug 5.8 grams to granule.Self-control.Batch number: 20030519.Date of manufacture: 20030519).
2. experimental animal:
SPF level Male Kunming strain mice, body weight 18-24g before the experiment is provided by Chinese Academy of Sciences's Shanghai Experimental Animal Center.Free diet, illumination in 12 hours (early 7 up to late 7 o'clock), 20 ± 2 ℃ of room temperatures.
(2) test method:
Measure autonomic activities in the mice 5 minutes first day morning, filter out the higher mice of autonomic activities number and be divided into 6 groups at random, be respectively normal group, model group, western medicine group, Chinese medicine matched group, the heavy dose of group of the granule of soothing the spirit, the granule small dose group of soothing the spirit, 12 every group.From second day continuous irrigation stomach two weeks, to give respectively and relative medicine, normal group and model group are given commensurability normal saline.
Measure autonomic activities number in the mice 5 minutes morning last day, administration then, carrying out cornea in 6 hours stimulates.After the stimulation mice is placed in the observation ward, observes and write down the accumulative total dead time of bringing out in the 5min.
(3) result of the test:
Experimental result sees Table 3, table 4.Model group and normal group compare, the dead time significant prolongation that the electricity irritation cornea brings out; The heavy dose of group of the granule of soothing the spirit compares with model group, can obviously shorten the dead time, and difference is extremely remarkable; Soothe the spirit granule small dose group, fluoxetine group and model group relatively, significantly shorten the dead time; Depression alleviating pills group and model group be there was no significant difference relatively.The autonomic activities of each administration group all with the model group there was no significant difference.
Table 3 medicine is to the influence of mice autonomic activities
| Group | Dosage | Number of animals | Autonomic activities number before the administration | Autonomic activities number after the administration |
| Normal group | 20mL/kg | 12 | 157.67±22.35 | 179.00±26.90 |
| Model group | 20mL/kg | 12 | 155.83±23.41 | 179.80±23.76 |
| The heavy dose of group of the granule of soothing the spirit | 3.9g/kg | 12 | 158.75±21.88 | 156.90±17.83 |
| The granule small dose group of soothing the spirit | 1.9g/kg | 12 | 156.00±23.33 | 171.30±20.98 |
| Depression alleviating pills | 1.5g/kg | 12 | 158.00±21.51 | 169.80±13.55 |
| Fluoxetine | 2.5mg/kg | 12 | 157.17±21.79 | 170.30±29.01 |
Table 4 medicine brings out the influence of dead time to electricity irritation mice cornea
| Group | Dosage | Number of animals | Dead time (s) |
| Normal group | 20mL/kg | 12 | 120.75±60.33* |
| Model group | 20mL/kg | 12 | 182.17±50.96 |
| The heavy dose of group of the granule of soothing the spirit | 3.9g/kg | 12 | 120.25±44.81** |
| The granule small dose group of soothing the spirit | 1.9g/kg | 12 | 138.83±35.98* |
| Depression alleviating pills | 1.5g/kg | 12 | 150.83±53.03 |
| Fluoxetine | 2.5mg/kg | 12 | 129.33±65.10* |
Compare with model group: * P＜0.05, * * P＜0.01.
Test example 4 (medicine of the present invention is learnt the influence of helpless behavior to rat)
(1) test material:
1. medicine:
(prozac is in fluoxetine 20mg/ grain for fluoxetine hydrochloride capsules.Factory: Eli Lilly CompanyLimited.Batch number: A042238.Date of manufacture: 2003.09.11).
(per 15 balls weigh 1 gram to depression alleviating pills.Yumi Pharmaceutical Co., Ltd., Zhengzhou.Batch number: 20030602.Date of manufacture: 2003.06.25).
Soothing the spirit, (every gram granule is equivalent to crude drug 5.8 grams to granule.Self-control.Batch number: 20030519.Date of manufacture: 20030519).Get an amount of granule and increase the weight of to steam water and be mixed with the suspension that concentration is 135.00mg/mL, 67.70mg/mL respectively, as large and small dosage sample liquid, refrigerator is preserved.
2. laboratory animal:
SPF level SD male rat, body weight 150-170g before the experiment is provided by Chinese Academy of Sciences's Shanghai Experimental Animal Center.Free diet, illumination in 12 hours (early 7 up to late 7 o'clock), 20 ± 2 ℃ of room temperatures.
(2) experimental technique:
Rat is divided into 6 groups at random, is respectively normal group, model group, western medicine group, Chinese medicine matched group, the heavy dose of group of the granule of soothing the spirit, the granule small dose group of soothing the spirit, 8 every group.Irritate stomach respectively and give and relative medicine, normal group and model group are given commensurability normal saline.
Carry out the galvanic shock of inevitability in first day and promptly make preceding shock animals.All chronic administration methods are taked in beginning administration in second day.The 24h condition of carrying out is avoided experiment after the last administration.Testing index is the incubation period of avoiding number of success and escape.
(3) result of the test:
Experimental result sees Table 5.Model group and normal group compare, and post-stimulatory escape latency obviously prolongs, and difference is extremely remarkable, avoid the number of success there was no significant difference; Fluoxetine group and model group compare, and escape latency obviously shortens, and difference is extremely remarkable; Large and small dosage group of the granule of soothing the spirit and model group compare, and escape latency has significance to shorten; Depression alleviating pills and model group be there was no significant difference relatively.Each administration group and model group are relatively avoided number of success there are no significant difference.
Table 5 medicine is learnt the influence of helpless state to rat
| Group | Dosage | Number of animals | Avoid number of success | Escape latency (s) |
| Normal group | 20mL/kg | 8 | 1.25±1.03 | 7.61±2.75** |
| Model group | 20mL/kg | 8 | 0.63±0.74 | 16.76±7.46 |
| The heavy dose of group of the granule of soothing the spirit | 2.7g/kg | 8 | 1.13±0.83 | 8.28±3.84* |
| The granule small dose group of soothing the spirit | 1.3g/kg | 8 | 0.75±0.71 | 9.65±2.77* |
| Depression alleviating pills | 1.1g/kg | 8 | 0.88±0.83 | 10.19±7.07 |
| Fluoxetine | 1.8mg/kg | 8 | 0.88±0.83 | 8.18±2.96** |
Compare with model group: * P＜0.05, * * P＜0.01.
Test example 5 (medicine of the present invention is to the influence of monoamine neurotransmitter in the brain)
(1) test material:
(Serotonin hydrochloride is available from sigma company, lot number: 112K7002) for 5-HT, 5-Hydroxytryptamine for 5-hydroxy tryptamine.
Norepinephrine (NA, NE, (±) Arterenol, (±) Norepinephrine-hydrogentartrate bitartrate salt is available from sigma company, lot number: 013K1281).
(DA, Dopamine hydrochloride is available from sigma company, lot number: 033K1192) for dopamine.
0.1mol/L phosphate buffer, normal heptane, n-butyl alcohol, 0.1mol/L iodine reagent, alkaline sodium sulfite solution, 0.1mol/L EDTA solution (pH7.0), OPT (o-phthalaldehyde(OPA)) hydrochloric acid solution.
(2) test method:
Draw NA, DA, 5-HT standard curve, in order to quantitatively.
The rat of finishing the helpless experiment of study is breaked end, gets brain rapidly, remove meninges and blood vessel on the ice platform, tell frontal cortex.Adopt solvent extraction method to separate NA, DA and 5-HT.Draw phosphate buffer and measure NA and DA content with the iodine reagent oxidizing process; Inhaling HCl solution contracts and method mensuration 5-HT with OPT.
(3) experimental result:
Medicine sees Table 6 to the influence of neurotransmitter content in the rat frontal cortex layer.Model group and normal group compare, and NA, DA, the equal significance of 5-HT content reduce.Each medication group and model group compare, and NA, DA, 5-HT content all have in various degree to be increased.The heavy dose of 5-HT content of organizing of the granule of wherein soothing the spirit, the DA and the 5-HT content of fluoxetine group all are significantly increased than model group, and difference is extremely remarkable.The NA content of the 5-HT content of the DA content of the heavy dose of group of granule, the granule small dose group of soothing the spirit of soothing the spirit, the 5-HT content of depression alleviating pills group, fluoxetine group all has significance to increase than model group.The NA and the DA content of the NA of the NA content of the heavy dose of group of granule, the granule small dose group of soothing the spirit of soothing the spirit and DA content, depression alleviating pills group all have increase than model group, but not statistically significant.
Table 6 medicine is to the influence of neurotransmitter content in the rat frontal cortex layer
| Group | Number of animals | NA content | DA content | 5-HT content |
| Normal group | 8 | 241.85±65.09* | 2480.57±314.95* | 1.52±0.22* |
| Model group | 8 | 157.40±59.06 | 1991.33±458.87 | 1.23±0.27 |
| The heavy dose of group of the granule of soothing the spirit | 8 | 216.84±55.88 | 2644.90±470.29* | 1.59±0.14** |
| The granule small dose group of soothing the spirit | 8 | 212.97±97.96 | 2518.46±1100.36 | 1.49±0.13* |
| Depression alleviating pills | 8 | 187.62±107.34 | 2151.89±575.57 | 1.58±0.31* |
| Fluoxetine | 8 | 219.72±51.57* | 2897.17±692.40** | 1.68±0.33** |
Compare with model group: * P＜0.05, * * P＜0.01.
Pharmacodynamic test of active extract shows that (3.9g/kg 1.9g/kg) can significantly shorten the mice non-swimming time to the granule of soothing the spirit; Soothing the spirit, (2.7g/kg 1.3g/kg) can significantly increase chronic stress and support the level of combination model rat and move both vertically with lonely granule; The granule (3.9g/kg, 1.9g/kg) of soothing the spirit can significantly shorten the motionless state time that electricity irritation mice cornea causes; Soothing the spirit, (2.7g/kg 1.3g/kg) can significantly shorten the escape latency of learning helpless rat model to granule; The granule (2.7g/kg) of soothing the spirit significantly to raise DA in the rat frontal cortex, 5-HT content, the 1.3g/kg 5-HT content that can significantly raise.Has antidepressant effect.
Test example 6 (clinical observation of Drug therapy depression of the present invention)
1. diagnostic criteria
Depressed continuing has four in following nine can be diagnosed as primary disease more than two weeks: hebetude, and energy goes down, and behavior is blunt, from light self-accusation, slowness of thinking, passiveness is committed suiside, sleep disorder, inappetence, hyposexuality.Depressive emotion heavy night in morning gently is an important feature.
Can be divided into following five types according to the differentiation of tcm primary disease: liver qi stagnation type, stagnation of QI phlegm stagnancy type, the melancholy type of overtaxing one's nerves, type of deficiency of both the heart and spleen, type of hyperactivity of fire caused by deficiency of YIN.
We cure mainly: the melancholy type of overtaxing one's nerves.
(1) mental symptom aspect: mental derangement is indefinite, the language and behavior sensory disorder, and as crouch unable to lie in bed, tending to walk but failing to do it of desire speech, desire quietly, or sad without reason, intemperance of emotions, vexed uneasiness, night sleep not good enough, or dimly the change irregularly border of parade of dream.
(2) as if subjective symptoms is many, and narration condition of illness is intact, and the whole body is all sick, no coldness but like cold syndrome, no fever but like febrile disease, or body such as ant foot sample, worries in the extreme, but the health check-up disease is few, does not have remarkable pathological changes.
(3) diet aspect: inappetence, stomach is received excellently sometimes, detests feed sometimes again, dry mouth with bitter taste, or constipation, it is red to urinate.
(4) arteries and veins tongue aspect: thready and rapid pulse, the few tongue of red tongue, or the little Huang of thin fur.Wherein, the 1st～2 is main dialectical foundation, and the 3rd～4 is accessory dialectical foundation, as long as 1 main, 1 less important is available we when facing card.
2. criterion of therapeutical effect:
The metering scoring:
4 minutes: spirit depressing, irritable emotion, worrying too much proof such as was difficult to resolve, forgetful insomnia, tongue arteries and veins are unusual and shows, and continued to occur cisco unity malfunction and give birth to the reviver.
3 minutes: proofs such as spirit depressing, forgetful insomnia, tongue arteries and veins are unusual showed, and often occurred, and influenced work and gave birth to the reviver.
2 minutes: heavy when light during cards such as irritable emotion, forgetful insomnia, tongue arteries and veins are unusual, be interrupted and occur, do not influence work and give birth to the reviver.
1 minute: cards such as depressed, sighing frequently, tongue arteries and veins are unusual were lighter, occur once in a while, do not influence work and give birth to the reviver.
0 minute: no syndrome or syndrome disappearance person.
Efficacy evaluation:
Produce effects: syndrome all disappears, integration be 0 or treatment before and after poor 〉=70% of syndrome integration.
Effectively: poor 〉=50% of syndrome integration before and after the treatment and＜70%.
Invalid: poor＜50% of syndrome integration before and after the treatment.
Increase the weight of: treatment back syndrome integration surpasses treats the former.
3. therapeutic outcome
Observe patient's 28 examples
Produce effects: 28.6%
Effectively: 53.6%
Invalid: 17.8%
Claims (8)
1. Chinese medicine composition for the treatment of depression, it is characterized in that it is prepared from medicament by following bulk drugs: 6～12 parts of Radix Salviae Miltiorrhizaes, 10～20 parts of Bulbus Liliies, 10～20 parts in Fructus Jujubae, 4～8 parts of Radix Glycyrrhizae Preparatas, 20～40 parts of Huai wheats, 8～16 parts in the Radix Rehmanniae.
2. according to the Chinese medicine composition of the treatment depression of claim 1, it is characterized in that it is the medicament that the feedstock production by following weight parts forms: 9 parts of Radix Salviae Miltiorrhizaes, 15 parts of Bulbus Liliies, 15 parts in Fructus Jujubae, 6 parts of Radix Glycyrrhizae Preparatas, 30 parts of Huai wheats, 12 parts in the Radix Rehmanniae.
3. the Chinese medicine composition of treatment depression according to claim 1 and 2 is characterized in that, described medicament is a said dosage form on any pharmaceutics.
4. the Chinese medicine composition of treatment depression according to claim 3 is characterized in that, described medicament is granule or oral liquid.
5. the preparation method of the Chinese medicine composition of claim 1 or 2 described treatment depressions is characterized in that, comprises the following steps:
(a) take by weighing Radix Salviae Miltiorrhizae, Bulbus Lilii, Fructus Jujubae, Radix Glycyrrhizae Preparata, Huai wheat and the Radix Rehmanniae;
(b) decocting that adds 8 times of amounts boils twice, decocts 1 hour at every turn, merges decocting liquid twice, filters;
(c) filtrate being concentrated into relative density is 1.20, with 90%～95% ethanol precipitate with ethanol;
(d) reclaim ethanol and to be concentrated into relative density be 1.36, vacuum drying under 60 ℃～80 ℃ condition, dry extract.
6. according to the preparation method of the Chinese medicine composition of the described treatment depression of claim 5, it is characterized in that the baking temperature of step (d) is 70 ℃.
7. treat the preparation method of the Chinese medicine composition of depression according to claim 5, it is characterized in that, the dry extract that wherein step (d) is made is pulverized, and add dextrin and mix, be wetting agent with ethanol, granulate, drying, granulate promptly makes granule.
8. the described Chinese medicine composition of claim 1-2 is in the application in the antidepressant medicine of preparation treatment.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013013498A1 (en) * | 2011-07-23 | 2013-01-31 | 江西食方食坊中药食品有限公司 | Chinese medicine beverage for nourishing the heart to calm the mind |
| CN103212019A (en) * | 2012-12-10 | 2013-07-24 | 宋爱民 | Chinese materia medica preparation for treating depression |
| CN103705774A (en) * | 2013-12-30 | 2014-04-09 | 江苏省中医院 | Compound composition with effect of treating depression as well as preparation method and application thereof |
| CN108323656A (en) * | 2018-01-23 | 2018-07-27 | 佛山科学技术学院 | Ganmai Chinese date beverage and preparation method thereof |
| CN110433253A (en) * | 2018-05-03 | 2019-11-12 | 杭州市妇产科医院 | A kind of liquorice-wheat-jujube particle and its preparation method and application |
-
2006
- 2006-11-22 CN CN200610118617A patent/CN100586465C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013013498A1 (en) * | 2011-07-23 | 2013-01-31 | 江西食方食坊中药食品有限公司 | Chinese medicine beverage for nourishing the heart to calm the mind |
| CN103212019A (en) * | 2012-12-10 | 2013-07-24 | 宋爱民 | Chinese materia medica preparation for treating depression |
| CN103212019B (en) * | 2012-12-10 | 2014-10-22 | 宋爱民 | Chinese materia medica preparation for treating depression |
| CN103705774A (en) * | 2013-12-30 | 2014-04-09 | 江苏省中医院 | Compound composition with effect of treating depression as well as preparation method and application thereof |
| CN108323656A (en) * | 2018-01-23 | 2018-07-27 | 佛山科学技术学院 | Ganmai Chinese date beverage and preparation method thereof |
| CN110433253A (en) * | 2018-05-03 | 2019-11-12 | 杭州市妇产科医院 | A kind of liquorice-wheat-jujube particle and its preparation method and application |
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| Publication number | Publication date |
|---|---|
| CN100586465C (en) | 2010-02-03 |
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