CN101185464A - Method for extracting coconut oil from coconut bran - Google Patents
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Abstract
The invention discloses a method used for extracting coconut oil from a coconut cake, and the steps are: one or more than two of cellulose, hemicellulase or papain are combined to make into enzyme water solution with which the coconut cake is mixed and stirred, kept warm, stirred with warm water and further grinded by a colloid mill; serous fluid obtained obtains thin coconut cream after going through tailings separation; the thin coconut cream goes through layers separation after placed stilly; a free oil layer, an emulsion layer, a serum layer and a precipitation bed are taken out respectively; the obtained free oil layer and the emulsion layer are mixed together to obtain oil emulsion mixture in which water and salt are added, and then the papain is added for continuing zymolysis and the salt is added for supplementing; the mixture is placed stilly to lead the free oil to float upward to get the coconut oil; the coconut oil is extract out, washed by water and filtered, and high-quality coconut oil with the water content less than or equal to 0.1 percent is obtained after going through vacuum drying. The invention has simple technique process, low production cost, high oil production rate, little pollution, high product quality and high comprehensive utilization value of byproducts.
Description
Technical field
The present invention relates to a kind of method of extracting coconut oil, be specifically related to a kind ofly from coconut cake, extract coconut oil and utilize the physicochemical properties of salt to improve the enzymatic extracting method of coconut oil by enzyme hydrolysis.
Background technology
Existing coconut oil extracts the preparation method and comprises dry process technology and wet method processing technology.Dry process technology is earlier coconut meat to be exposed to high temperature or following a period of time of sunlight, makes copra until removing most moisture, with oil press the copra squeezing is obtained coconut oil again.The technology of copra oil expression has developed into the method that cold press combines with solvent extraction in recent years, can significantly improve oil yield.The press apparatus that uses, then hydraulic oil press from rotary oil press to the modern times and continuous screw oil press etc., but these equipment investment cost height, power consumption is more, and is also higher to the requirement of Operation and maintenance technology, uses solvent extraction can cause environmental pollution simultaneously.In addition, because copra is often contaminated and send putrefactive odor, the thick cocounut oil poor quality who obtains often has peculiar smell, therefore generally need to handle with production refining, bleaching and deodorizing coconut oil (RBD) through refining, bleaching and deodorizing, and RBD experiences machinery, chemistry and high-temperature process several times, has significant yellow, and is salty slightly, almost there is not the coconut aromatic odor, the nutritional labeling loss of product is bigger, and a kind of offending smell is also arranged usually, so the quality of gained coconut oil is not high.
Modern wet method processing technology extracts the method for coconut oil, be to be raw material with fresh coconut meat, squeezing obtains coconut milk (coconut juice), with the coconut milk heating, separate then, coconut milk is divided into components such as coconut milk junket, degreasing coconut milk and sludge with liquid-centrifuge of fluid, again with heating of coconut milk junket and inspissation, separate with the solid-liquid centrifuge then, isolated muddy coconut oil with the liquid-liquid centrifuge clarification, makes the final products coconut oil more after drying again.The coconut oil quality that wet method processing technology is made is better than the coconut oil of dry process gained, but high-speed centrifuge costs an arm and a leg, and equipment investment is big, is unfavorable for applying.
Also have at present with coconut milk spontaneous fermentation more than one day reusable heat handle and centrifugal separation is extracted coconut oil, but this method oil yield is low, and separates other accessory substances after the coconut oil because the rancid utilization that is difficult to of going bad.
The patent No. is that 98110506.8 " a kind of method of extracting cocounut oil and byproduct from fresh coconut " is coconut milk to be sent in the air drier moisture by the high temperature evaporation coconut milk separate and obtain coconut oil, because hot blast temperature is between 135 ~ 165 ℃, can damage the thermal sensitivity composition in the coconut oil, caramelization takes place simultaneously, produces caramel.
In laboratory scope, be widely used in the oil of carrying of the multiple oilseed that comprises coconut with the pretreated method of enzyme, and in commerical test, obtained some effects.Though existing enzyme method for pretreating can improve oil yield, but because the mild condition that enzyme is handled, enzymolysis time is long, efficient is low, various microorganisms are easy to growth and breeding in the enzyme processing procedure, particularly putrid microorganism is easy to growth and breeding and makes zymolyte rotten smelly under neutral and subalkaline condition, even can cause becoming sour of grease, and this is that the major reason that oil process seldom drops into industrialized production is put forward in present enzyme processing.
Number of patent application be CN's 01807991.1 " producing the method for enzymatically treating of the coconut meat of edible product ", by pectase, cellulase, hemicellulase and the carbohydrase coconut meat of degrading, again by the centrifugal coconut wet goods composition that obtains of centrifuge, this technology needs the high speed centrifugation separation equipment equally, employed enzyme dosage is bigger than normal, the production cost height, used more chemical reagent in the production process, and the control microbial growth breeding of also not adopting an effective measure in the enzymolysis process has harmful effect to product quality.
In the coconut process, with shell, peel, coconut meat after the fragmentation is squeezed the juice the acquisition coconut milk, is used for the production of coconut food such as coconut milk, coconut powder, coconut magma, candy and cocoanut jam.In suitability for industrialized production, even adopt the high performance equipment of squeezing the juice, the wet coconut cake of the accessory substance after squeezing the juice still accounts for about 40% (weight in wet base) of raw material coconut meat, and wet coconut cake moisture is about 40%, fat (coconut oil) content 40%～50% (butt meter).Though wet coconut cake is sold through can be used as the shredded coconut stuffing of squeezing the juice behind the boiling and drying, it is cheap, and added value is low.The physicochemical property of the coconut oil in the coconut oil in the fresh wet coconut cake and the bright coconut meat is identical, can be used as the high-quality coconut oil raw material that extracts high added value.
Summary of the invention
The purpose of this invention is to provide a kind of method of from coconut cake, extracting coconut oil, its used enzyme preparation consumption is few, equipment is simple, oil yield is high, technology is polluted less, superior product quality, byproduct comprehensive value height, production cost are low, is that a kind of coconut cake with cheapness is the process that raw material extracts the high-quality coconut oil of preparation.
The present invention obtains according to following principle:
One, because the byproduct coconut cake of coconut meat after squeezing the juice has very strong water imbibition, mixes with coconut cake after enzyme aqueous solution is made in a kind of or its combination of cellulase, hemicellulase, papain, enzyme can by coconut cake rapidly absorption enter interior tissue.Because cellulase is difficult to purify, commercially available cellulase also contains the hemicellulase enzyme relevant with other usually, as amylase, protease etc.; Hemicellulase comprises zytase, 1,4 beta-glucanase, Galactanase and mannase or its mixture; Papain yet contains cellulase, cysteine proteinase etc. except that containing papain.These enzymes are absorbed and enter hemicellulose, cellulose, protein and the starch that the coconut cake internal coordination decomposes cell tissue, can improve the yield of extracting rare coconut palm slurry from coconut cake very significantly.
The present invention can adjust the combination of enzyme according to the target purposes of the accessory substance that will obtain when handling coconut cake:
1, obtains to be rich in the edible cocoanut fiber of protein if desired, do not need to add protease at enzyme aqueous solution;
2, improve the degreasing coconut milk that protein is rich in rare coconut palm slurry recovery rate and acquisition if desired to greatest extent, in enzyme aqueous solution, add protease.
Discover through the inventor that two, the coconut palm slurry emulsification system that papain and salt synergy are applied to handle oil-containing and protein has the incomparable superiority of other protease to extract coconut oil:
1, but the pH sphere of action of papain catalytic reaction is 3.5～9.0, and optimum pH 5.0～7.0 is consistent with natural pH (pH5.8～6.3) scope of coconut palm slurry, need not to add acid, alkali or cushioning liquid and adjusts pH, avoids using chemical additive.
2, papain also contains cellulase, cysteine proteinase (decompose the cysteine that produces papain is had activation) etc. except that containing papain, can destroy the emulsification system of coconut palm slurry effectively, accelerates separating out of free oil.
3, papain also contains lysozyme, and some bacteriums are had killing action.
4, the papain maximum temperature that can tolerate is 70 ℃, can handle the coconut palm slurry under higher temperature, has not only improved the efficient of enzymolysis but also can suppress the growth and breeding of corruptibility bacterium, mould and yeast, has guaranteed the freshness of coconut oil and degreasing coconut milk.
5, papain has salt tolerance and can be activated by sodium ion, chlorion, adds salt when handling the coconut palm slurry, both can be used as the activator of papain, can suppress the growth and breeding of corruptibility bacterium again.Especially meaningfully, in the system that contains oil phase, water and solid phase, add salt, can increase the density contrast between oil phase and other phases, accelerate the separating rate of oil phase and water, and, because the salting out of protein, energy promotion oil separates with protein, improves the recovery rate of coconut oil.
6, coconut protein is not produced the enzymolysis product that contains bitter taste by the papain decomposition, and gained degreasing coconut milk better flavor can be as the raw material of food industry.
Three, the hemicellulose in the coconut cake, protein are decomposed into the enzymolysis product of biologically actives such as oligosaccharide, monose, little peptide and amino acid and health care respectively under the effect of hemicellulase and protease, from coconut cake, discharge and enter in rare coconut palm slurry, with rare coconut palm slurry standing demix, obtain being rich in the whey layer and the beds of precipitation of these nutriments, its merging is become the degreasing coconut milk, can be used as the raw material of functional food industry.
The designed method of extracting coconut oil from coconut cake of the present invention is:
1, enzyme aqueous solution is made in one or more combinations in cellulase, hemicellulase or the papain,, then added the activator of salt simultaneously in enzyme aqueous solution as papain if papain is arranged; Coconut cake and enzyme aqueous solution that coconut meat is obtained after squeezing coconut milk mix, and insulation adds warm water and stirs, and further pulverizes homogenate with colloid mill, and the insulation of gained slurries is carried out the screenings separation then and obtained rare coconut palm slurry; Can be used as edible cocoanut fiber after the coconut palm slag drying; Rare coconut palm slurry left standstill be divided into 4 layers: free oil layer, emulsion layer, whey layer and the beds of precipitation.Free oil layer, emulsion layer (being rich in grease and protein), whey layer and the beds of precipitation are taken out respectively, and the whey layer and the beds of precipitation merge becomes the degreasing coconut milk, can be used as the raw material of food industry.Described hemicellulase is one or more mixing in zytase, 1,4 beta-glucanase, Galactanase or the mannase.
2, free oil layer and the emulsion layer merging that obtains obtained oily newborn mixture, in the newborn mixture of oil, add entry, add salt, add papain then and continue enzymolysis, then add salt again, the protein condenses precipitation leaves standstill and makes the limpid coconut oil of free oil come-up acquisition.The proteins precipitate that cohesion is separated out can further be processed into protein powder after reclaiming.
3, the upper strata coconut oil is taken out, add the water washing coconut oil, remove by filter suspension, obtain the high-quality coconut oil of moisture≤0.1% again through vacuum drying to remove residual salinity, enzyme and other impurity.
Can be used as edible cocoanut fiber after the coconut palm slag drying.
The whey layer and the beds of precipitation merge becomes the degreasing coconut milk, can be used as the raw material of food industry.
The proteins precipitate that cohesion is separated out can further be processed into protein powder after reclaiming.
It is provided by the present invention that the method technical process of extraction coconut oil is simple from coconut cake, production cost is low, equipment is simple, oil yield is high, prepared coconut oil does not need to handle through refining, bleaching and deodorizing, can farthest keep the nutritional labeling of natural coconut oil and natural flavour mountaineous, have the character more superior than refining coconut oil, technology is polluted less, superior product quality, byproduct comprehensive value height.
The specific embodiment
Below the invention will be further described.
The method of extracting coconut oil from coconut cake provided by the present invention is:
1, by the coconut cake mass ratio, one or more combinations in cellulase with 0.02%～1.00%, 0.02%～1.00% hemicellulase, 0.01%～0.50% the papain are got 0.5～3 times water by the coconut cake mass ratio then and are made enzyme aqueous solution; If papain arranged, then add 0.5%～10% salt activator by the coconut cake mass ratio simultaneously as papain in enzyme aqueous solution.Coconut cake and enzyme aqueous solution are mixed, 40～60 ℃ of insulations 2～6 hours, adding 1～10 times of temperature by the coconut cake mass ratio again is that 40～60 ℃ warm water stirs, further pulverize homogenate with colloid mill, the gained slurries carry out the screenings separation then and obtain rare coconut palm slurry 40～60 ℃ of insulations 0.5～5 hour; Can be used as edible cocoanut fiber after the coconut palm slag drying; Rare coconut palm slurry left standstill be divided into 4 layers from top to bottom after 1～4 hour: free oil layer, emulsion layer, whey layer and the beds of precipitation, free oil layer, emulsion layer (being rich in grease and protein), whey layer and the beds of precipitation are taken out respectively, the whey layer and the beds of precipitation merge becomes the degreasing coconut milk, can be used as the raw material of food industry.Described hemicellulase is one or more mixing in zytase, 1,4 beta-glucanase, Galactanase or the mannase.
2, free oil layer and emulsion layer merging are obtained oily newborn mixture, the water that in the newborn mixture of oil, adds 0～2 times by the newborn mixture weight of oil, adding salt makes the brine concentration of aqueous phase reach 2%～10%, add 0.01%～0.50% papain by the newborn mixture total weight of oil, continued enzymolysis 6～24 hours at 45～65 ℃, adding salt then makes the brine concentration of aqueous phase reach 10%～30%, the protein condenses precipitation leaves standstill and made the limpid coconut oil of free oil come-up acquisition in 3～24 hours.The proteins precipitate that cohesion is separated out can further be processed into protein powder after reclaiming.
3, the upper strata coconut oil is taken out, add 0.5～3 times water washing coconut oil 1～3 time to remove residual salinity, enzyme and other impurity by the total restatement of the coconut oil that obtains, remove by filter suspension, obtain the high-quality coconut oil of moisture≤0.1% again through vacuum drying.
Can be used as edible cocoanut fiber after the coconut palm slag drying.
The whey layer and the beds of precipitation merge becomes the degreasing coconut milk, can be used as the raw material of food industry.
The proteins precipitate that cohesion is separated out can further be processed into protein powder after reclaiming.
The invention will be further described with non-limiting embodiment below.Those skilled in the art can make amendment to technical parameter of the present invention as the case may be, as adjust the consumption of enzyme according to the enzyme activity index of commercial enzyme preparation.
Example one, be that the cellulase of 10000U/g is dissolved in the 10kg water and makes enzyme aqueous solution with the 15g enzyme activity unit, mix squeezing fresh wet coconut cake of the 10kg that obtains after the coconut milk and enzyme aqueous solution, 50 ℃ of insulations 3 hours, the warm water of adding 50 ℃ of 30kg then stirs, further pulverize homogenate with colloid mill, the gained slurries carry out the screenings separation with milling process and obtain coconut palm slag and rare coconut palm slurry 50 ℃ of insulations 2 hours.Rare coconut palm slurry left standstill be divided into 4 layers after 2 hours: free oil layer, emulsion layer, whey layer and the beds of precipitation.Free oil layer, emulsion layer and whey layer and the beds of precipitation are taken out respectively.Free oil layer and emulsion layer merging are obtained the newborn mixture of about 4kg oil, in the newborn mixture of oil, add 2kg water, add salt and make the brine concentration of aqueous phase reach 5%, the papain of adding the 8g enzyme activity unit and be 1000000U/g continued enzymolysis 24 hours at 55 ℃.Add salt then and make the brine concentration of aqueous phase reach 20%, the protein condenses precipitation leaves standstill and made the limpid coconut oil of free oil come-up acquisition in 6 hours.The upper strata coconut oil is taken out, and the water washing coconut oil 2 times that adds 1 times removes by filter suspension to remove residual salinity, enzyme and other impurity.Vacuum drying obtains the high-quality coconut oil of 1.5kg moisture≤0.1% under 60 ℃, the condition of vacuum 0.07～0.08MPa.
Example two, be that the zytase of 10000U/g, papain, 50g salt that the 5g enzyme activity unit is 1000000U/g are dissolved in the 10kg water and make enzyme aqueous solution with the 10g enzyme activity unit, mix squeezing fresh wet coconut cake of the 10kg that obtains after the coconut milk and enzyme aqueous solution, 55 ℃ of insulations 3 hours, the warm water of adding 50 ℃ of 30kg then stirs, further pulverize homogenate with colloid mill, the gained slurries carry out the screenings separation with milling process and obtain coconut palm slag and rare coconut palm slurry 45 ℃ of insulations 1 hour.Rare coconut palm slurry left standstill be divided into 4 layers after 1 hour: free oil layer, emulsion layer, whey layer and the beds of precipitation.Free oil layer, emulsion layer and whey layer and the beds of precipitation are taken out respectively.Free oil layer and emulsion layer merging are obtained the newborn mixture of about 4.5kg oil, in the newborn mixture of oil, add 4kg water, adding salt makes the brine concentration of aqueous phase reach 5%, adding the 5g enzyme activity unit is that the 1000000U/g papain continued enzymolysis 24 hours at 55 ℃, adding salt then makes the brine concentration of aqueous phase reach 20%, the protein condenses precipitation leaves standstill and made the limpid coconut oil of free oil come-up acquisition in 6 hours.The upper strata coconut oil is taken out, and the water washing coconut oil 2 times that adds 1 times removes by filter suspension to remove residual salinity, enzyme and other impurity.Vacuum drying obtains the high-quality coconut oil of 1.65kg moisture≤0.1% under 60 ℃, the condition of vacuum 0.07～0.08MPa.
Example three, with the 10g enzyme activity unit is the cellulase of 10000U/g, the 10g enzyme activity unit is the zytase of 10000U/g, the 5g enzyme activity unit is the 1,4 beta-glucanase of 10000U/g, the 5g enzyme activity unit is the papain of 1000000U/g, 40g salt is dissolved in the 10kg water makes enzyme aqueous solution, mix squeezing fresh wet coconut cake of the 10kg that obtains after the coconut milk and enzyme aqueous solution, 50 ℃ of insulations 2 hours, the warm water of adding 50 ℃ of 30kg then stirs, further pulverize homogenate with colloid mill, the gained slurries carry out the screenings separation with milling process and obtain coconut palm slag and rare coconut palm slurry 45 ℃ of insulations 1 hour.Rare coconut palm slurry left standstill be divided into 4 layers after 1 hour: free oil layer, emulsion layer, whey layer and the beds of precipitation.Free oil layer and emulsion layer merging are obtained the newborn mixture of about 4.8kg oil, in the newborn mixture of oil, add 4kg water, adding salt makes the brine concentration of aqueous phase reach 5%, adding the 5g enzyme activity unit is that the 1000000U/g papain continued enzymolysis 24 hours at 50 ℃, adding salt then makes the brine concentration of aqueous phase reach 20%, the protein condenses precipitation leaves standstill and made the limpid coconut oil of free oil come-up acquisition in 6 hours.The upper strata coconut oil is taken out, and the water washing coconut oil 2 times that adds 1 times removes by filter suspension to remove residual salinity, enzyme and other impurity.Vacuum drying obtains the high-quality coconut oil of 1.9kg moisture≤0.1% under 60 ℃, the condition of vacuum 0.07～0.08MPa.
Example four, the 10g enzyme activity unit is the zytase of 10000U/g, 1,4 beta-glucanase that the 5g enzyme activity unit is 10000U/g, the 5g enzyme activity unit is 1000000U/g papain, 30g salt is dissolved in the 10kg water makes enzyme aqueous solution, dried coconut cake of 5kg and enzyme aqueous solution are mixed, 50 ℃ of insulations 3 hours, the warm water of adding 50 ℃ of 10kg then stirs, further pulverize homogenate with colloid mill, the gained slurries carry out the screenings separation with milling process and obtain coconut palm slag and rare coconut palm slurry 45 ℃ of insulations 1 hour.Rare coconut palm slurry left standstill be divided into 4 layers after 1 hour: free oil layer, emulsion layer, whey layer and the beds of precipitation.Free oil layer, emulsion layer and whey layer and the beds of precipitation are taken out respectively.Free oil layer and emulsion layer merging are obtained the newborn mixture of about 4.7kg oil, in the newborn mixture of oil, add 4kg water, adding salt makes the brine concentration of aqueous phase reach 5%, adding the 5g enzyme activity unit is that the 1000000U/g papain continued enzymolysis 24 hours at 55 ℃, adding salt then makes the brine concentration of aqueous phase reach 20%, the protein condenses precipitation leaves standstill and made the limpid coconut oil of free oil come-up acquisition in 6 hours.The upper strata coconut oil is taken out, and the water washing coconut oil 3 times that adds 2 times removes by filter suspension to remove residual salinity, enzyme and other impurity.Vacuum drying obtains the high-quality coconut oil of 1.8kg moisture≤0.1% under 60 ℃, the condition of vacuum 0.07～0.08MPa.
Claims (3)
1. method of extracting coconut oil from coconut cake is characterized in that comprising following process:
1), by the coconut cake mass ratio, one or more combinations in cellulase with 0.02%～1.00%, 0.02%～1.00% hemicellulase, 0.01%～0.50% the papain are got 0.5～3 times water by the coconut cake mass ratio then and are made enzyme aqueous solution; Coconut cake and enzyme aqueous solution are mixed, 40～60 ℃ of insulations 2～6 hours, adding 1～10 times of temperature by the coconut cake mass ratio again is that 40～60 ℃ warm water stirs, further pulverize homogenate with colloid mill, the gained slurries carry out the screenings separation then and obtain rare coconut palm slurry 40～60 ℃ of insulations 0.5～5 hour; Rare coconut palm slurry left standstill be divided into free oil layer, emulsion layer, whey layer and the beds of precipitation from top to bottom after 1～4 hour, free oil layer, emulsion layer, whey layer and the beds of precipitation are taken out respectively, the whey layer and the beds of precipitation merge becomes the degreasing coconut milk, can be used as the raw material of food industry;
2), free oil layer and emulsion layer merging are obtained oily newborn mixture, the water that in the newborn mixture of oil, adds 0～2 times by the newborn mixture weight of oil, adding salt makes the brine concentration of aqueous phase reach 2%～10%, add 0.01%～0.50% papain by the newborn mixture total weight of oil, continued enzymolysis 6～24 hours at 45～65 ℃, adding salt then makes the brine concentration of aqueous phase reach 10%～30%, the protein condenses precipitation leaves standstill and made the limpid coconut oil of free oil come-up acquisition in 3～24 hours;
3), the upper strata coconut oil is taken out, add 0.5～3 times water washing coconut oil 1～3 time to remove residual salinity, enzyme and other impurity by the total restatement of the coconut oil that obtains, remove by filter suspension, obtain the high-quality coconut oil of moisture≤0.1% again through vacuum drying.
2. the method for extracting coconut oil from coconut cake according to claim 1, it is characterized in that: described hemicellulase is one or more mixing in zytase, 1,4 beta-glucanase, Galactanase or the mannase.
3. the method for extracting coconut oil from coconut cake according to claim 1 is characterized in that: when in the enzyme aqueous solution papain being arranged, then add 0.5%～10% the salt activator as papain by the coconut cake mass ratio simultaneously.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204648A (en) * | 2011-04-11 | 2011-10-05 | 中国热带农业科学院椰子研究所 | Method for preparing modified coconut dietary fiber chewable tablets by using coconut bran as raw material |
| CN103205309A (en) * | 2013-03-22 | 2013-07-17 | 西北师范大学 | Method for preparing sorbifolia seed kernel oil by enzyme-coupled salt method |
| CN104194914A (en) * | 2014-07-01 | 2014-12-10 | 中国热带农业科学院椰子研究所 | Method of preparing natural coconut oil from solid coconut cream powder |
| CN105238543A (en) * | 2015-10-30 | 2016-01-13 | 海南大学 | Extraction method for coconut oil |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100548150C (en) * | 2007-03-09 | 2009-10-14 | 伍曾利 | The preparation method of coconut extractive |
-
2007
- 2007-10-25 CN CN200710184890A patent/CN100579377C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204648A (en) * | 2011-04-11 | 2011-10-05 | 中国热带农业科学院椰子研究所 | Method for preparing modified coconut dietary fiber chewable tablets by using coconut bran as raw material |
| CN102204648B (en) * | 2011-04-11 | 2013-06-05 | 中国热带农业科学院椰子研究所 | Method for preparing modified coconut dietary fiber chewable tablets by using coconut bran as raw material |
| CN103205309A (en) * | 2013-03-22 | 2013-07-17 | 西北师范大学 | Method for preparing sorbifolia seed kernel oil by enzyme-coupled salt method |
| CN104194914A (en) * | 2014-07-01 | 2014-12-10 | 中国热带农业科学院椰子研究所 | Method of preparing natural coconut oil from solid coconut cream powder |
| CN105238543A (en) * | 2015-10-30 | 2016-01-13 | 海南大学 | Extraction method for coconut oil |
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