CN101181648A - Preparation of renovation bracket containing natural spider silk and type i collagen nano fibrous tissue - Google Patents
Preparation of renovation bracket containing natural spider silk and type i collagen nano fibrous tissue Download PDFInfo
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- CN101181648A CN101181648A CNA2007101716220A CN200710171622A CN101181648A CN 101181648 A CN101181648 A CN 101181648A CN A2007101716220 A CNA2007101716220 A CN A2007101716220A CN 200710171622 A CN200710171622 A CN 200710171622A CN 101181648 A CN101181648 A CN 101181648A
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Abstract
The invention relates to a method for preparing tissue repair bracket containing natural cobweb and I type collagen nanometer fiber, comprising the steps: (1) the I type collagen is dissolved in hexafluoroisopropanol(HFIP), and mixed to be dissolved completely so as to prepare the filature liquor A; (2) the natural cobweb is dissolved in hexafluoroisopropanol, and mixed to be dissolved completely by slight heating so as to prepare the filature liquor B; (3) the clear and transparent liquor A and B are mixed according to a certain proportion; (4) the injecting pump for controlling the filature liquor is set and applied with voltage, and a grounding aluminum foil or a roller accepts the fiber silk at the pin point of the spinneret so as to get the disorder nanometer fiber membrane or nanometer fiber bracket. The nanometer fiber has even diameter, moderate porosity and favorable mechanical performance and biological compatibility, which can be used as the tissue repair bracket for blood vessel, skin and nerve and so on.
Description
Technical field
The invention belongs to the preparation of electrostatic spinning nano fiber, particularly relate to a kind of preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support.
Background technology
Tissue and organ that the organizational project recovery technique is decreased for disease provide a kind of new approach.In organizational project, the cytothesis support plays effects such as supporting cell growth, guide tissue regeneration and control organizational structure, is that the decision organizational project is repaired one of key of success factor.American Formhals had introduced the method for utilizing electrostatic repulsion to obtain the polymer fiber silk first in one piece of patent in 1934.
Type i collagen is a kind of natural polypeptides material that is widely studied in the organizational project, owing to itself be exactly the main component of mammalian cell epimatrix, result of study shows for many years: collagen satisfies as culture matrixes such as epidermis cell, fibroblast, chondrocytes.And as medical material, collagen has also been obtained a lot of achievements [Biochem Biophys Res Commun 2006,344 (1): 362-369. in application facet such as degradable suture, artificial skin, corneal injury treatments; Surface andCoatings Technology, In Press, Corrected Proof, Available online 24 August 2006], but single collagen-based materials is as the intravascular tissue engineering support, [Biomaterials 2000,21:997-1003 all to be difficult to satisfy the needs of cell culture on mechanical strength and the degradation speed; China's Tissue Engineering Study and clinical rehabilitation 2007,11 (1): 11-13].
The natural spider silk main component is a kind of specific proteins that is called the Aranea element, alanine (accounting for 25%) and glycine (accounting for 40%) during this proteinic aminoacid is formed, other also has several seed amino acids such as glutamate, Glu, serine, leucine, proline and tyrosine.Discovering that the protein molecular that contains alanine is arranged in wrinkle structure closely, is lenticular, is to cause the unusual firm reason of spider silk.And the protein molecular that contains glycine is arranged and to be seemed disorderly and unsystematic, thereby makes fabulous elasticity and dilatancy are arranged spider silk [Nature 2001,410; Biomacromolecules 2005,6:1405-1413; Materials Science and Engineering 2002, (6): 30-32].Its outstanding mechanical performance is suitable as tissue engineering bracket material.But at present very few for its bio-safety Journal of Sex Research as timbering material, key constraints is its source.
Collagen and spider silk all are natural polypeptidess, compare other artificial polymeric material, have high-biocompatibility and low immunologic rejection.Up to now, static spins spider silk-collagen composite nano material and yet there are no report.By advantage in conjunction with Aranea and collagen, utilize both high-pressure electrostatic blending, be expected to filter out a kind of existing high-strength mechanical performance, the three-dimensional porous rack material of good biocompatibility is arranged again.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method that contains natural spider silk and type i collagen nano fibrous tissue recovery support, by with natural material and the compound novel nano fiber that obtains having shell-and-core structure of polymer material coaxial, this kind nano-fiber material has better biocompatibility and mechanical performance simultaneously, can be used as tissue engineering bracket material.
A kind of preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support of the present invention comprises the following steps:
(1) natural spider silk is dissolved in the hexafluoroisopropanol (HFIP), and slight fever is stirred to dissolving fully, preparation spinning solution A;
(2) type i collagen is dissolved in the hexafluoroisopropanol (HFIP), is stirred to dissolving fully, spinning solution B;
(3) set the syringe pump of controlling blend spinning solution, apply voltage, ground connection aluminium foil or roller bearing are accepted cellosilk at spinning head needle point place, get unordered nano-fiber film or nano fiber scaffold.
Described spinning solution A concentration is 3-8%;
Described spinning solution B concentration is 4-10%;
The volume ratio of described blend spinning solution concentration A and B is 3: 1,5: 5 or 1: 3;
The propelling speed of described blend spinning solution is 0.4-1.0mL/h;
Described electrostatic pressure is 16-20KV;
Described spinning head and receiver sheet distance are 10.0-14.0cm;
Described nanofiber is the nanofiber of 200-260nm.
Beneficial effect of the present invention:
(1) the nanometer recovery support fiber that makes of the present invention is even, and porosity is moderate, keeps the mechanical performance of fiber high-strength degree, appropriate hydrophilic and excellent biological compatibility;
(2) nanofiber of the present invention can be used as novel organizational project renovating bracket material, is tissue recovery support developing fields such as preparation extracellular matrix, skin blood vessel, skin, nerve.
Description of drawings
Fig. 1 is embodiment 1 prepared composite nano fiber stereoscan photograph (SEM, 1000 *);
Fig. 2 is embodiment 2 prepared composite nano fiber tubular bracket photos;
Fig. 3 is that embodiment 3 prepared composite nano fiber recovery support hydrophilic are measured water contact angle (CA) photo;
Fig. 4 is that embodiment 4 prepared composite nano fiber recovery support biocompatibility detect, vascular endothelial cell (PIEC) growth HE dyeing photo.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
Take by weighing the 0.30g spider silk with electronic analytical balance and be dissolved in the 5mL hexafluoroisopropanol, slight fever is stirred to dissolving fully, and obtaining concentration is the spider silk solution A of 6% (grams per milliliter); 0.40g collagen is dissolved in the 5mL hexafluoroisopropanol, is stirred to dissolving fully under the room temperature, obtaining concentration is the collagen spinning solution B of 8% (grams per milliliter); Begin electrostatic spinning after spinning solution A and B mixed with 1: 3 volume ratio, the syringe pump fltting speed of setting the control mixed solution is 0.8mL/h, spinnerette diameters is 1.2mm, applying voltage is 20KV, accept cellosilk in the place with aluminium foil 11cm under needle point of ground connection, form unordered nano-fiber film behind the 2h on the aluminium foil by the about 255nm of average diameter.
Embodiment 2
Take by weighing the 0.30g spider silk with electronic analytical balance and be dissolved in the 5mL hexafluoroisopropanol, slight fever is stirred to dissolving fully, and obtaining concentration is the spider silk solution A of 6% (grams per milliliter); 0.40g collagen is dissolved in the 5mL hexafluoroisopropanol, is stirred to dissolving fully under the room temperature, obtaining concentration is the collagen spinning solution B of 8% (grams per milliliter); Begin electrostatic spinning after spinning solution A and B mixed with 5: 5 volume ratios, the syringe pump fltting speed of setting the control mixed solution is 0.6mL/h, spinnerette diameters is 0.6mm, applying voltage is 20KV, accept cellosilk in the place with roller bearing 13cm under needle point of ground connection, form internal diameter 5mm, wall thickness 0.3mm, long 8cm nano-fiber tubular scaffold after 8 hours.
Embodiment 3
Take by weighing the 0.30g spider silk with electronic analytical balance and be dissolved in the 5mL hexafluoroisopropanol, slight fever is stirred to dissolving fully, and obtaining concentration is the spider silk solution A of 6% (grams per milliliter); 0.30g collagen is dissolved in the 5mL hexafluoroisopropanol, is stirred to dissolving fully under the room temperature, obtaining concentration is the collagen spinning solution B of 6% (grams per milliliter); Begin electrostatic spinning after spinning solution A and B mixed with 5: 5 volume ratios, the syringe pump fltting speed of setting the control mixed solution is 0.6mL/h, spinnerette diameters is 1.2mm, applying voltage is 18KV, the place accept cellosilk with the aluminium foil of ground connection at 11cm under needle point, form unordered nano-fiber film behind the 2h on the aluminium foil by the about 240nm of average diameter.
Embodiment 4
Take by weighing the 0.25g spider silk with electronic analytical balance and be dissolved in the 5mL hexafluoroisopropanol, slight fever is stirred to dissolving fully, and obtaining concentration is the spider silk solution A of 5% (grams per milliliter); 0.30g collagen is dissolved in the 5mL hexafluoroisopropanol, is stirred to dissolving fully under the room temperature, obtaining concentration is the collagen spinning solution B of 6% (grams per milliliter); Begin electrostatic spinning after spinning solution A and B mixed with 3: 1 volume ratios, the syringe pump fltting speed of setting the control mixed solution is 0.6mL/h, spinnerette diameters is 1.2mm, applying voltage is 18KV, the place accept cellosilk with the aluminium foil of ground connection at 11cm under needle point, form unordered nano-fiber film behind the 2h on the aluminium foil by the about 220nm of average diameter.
Claims (8)
1. a preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support comprises the following steps:
(1) natural spider silk is dissolved among the hexafluoroisopropanol HFIP, is stirred to dissolving fully, preparation spinning solution A;
(2) type i collagen protein solid granule is dissolved among the hexafluoroisopropanol HFIP, and slight fever is stirred to dissolving fully, preparation spinning solution B;
(3) clear solution A and B are mixed;
(4) set the syringe pump of controlling blend spinning solution, apply voltage, ground connection aluminium foil or roller bearing are accepted cellosilk at spinning head needle point place, get unordered nano-fiber film or nano fiber scaffold.
2. a kind of preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support according to claim 1 is characterized in that: described spinning solution A concentration is 3-8%.
3. a kind of preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support according to claim 1 is characterized in that: described spinning solution concentration B is 4-10%.
4. a kind of preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support according to claim 1, it is characterized in that: the volume ratio of described spinning solution concentration A and B is 3: 1,5: 5 or 1: 3.
5. a kind of preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support according to claim 1, it is characterized in that: the propelling speed of described blend spinning solution is 0.4-1.0mL/h.
6. a kind of preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support according to claim 1, it is characterized in that: described electrostatic pressure is 16-20KV.
7. a kind of preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support according to claim 1 is characterized in that: described spinning head and receiver sheet distance are 10.0-14.0cm.
8. a kind of preparation that contains natural spider silk and type i collagen nano fibrous tissue recovery support according to claim 1, it is characterized in that: described nanofiber is the nanofiber of 200-260nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101716220A CN101181648A (en) | 2007-11-30 | 2007-11-30 | Preparation of renovation bracket containing natural spider silk and type i collagen nano fibrous tissue |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101716220A CN101181648A (en) | 2007-11-30 | 2007-11-30 | Preparation of renovation bracket containing natural spider silk and type i collagen nano fibrous tissue |
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| CN101181648A true CN101181648A (en) | 2008-05-21 |
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| CNA2007101716220A Pending CN101181648A (en) | 2007-11-30 | 2007-11-30 | Preparation of renovation bracket containing natural spider silk and type i collagen nano fibrous tissue |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103046225A (en) * | 2012-01-19 | 2013-04-17 | 苏州达普生物技术有限公司 | Preparation method of collagen membrane |
| CN110129997A (en) * | 2019-06-05 | 2019-08-16 | 安徽迈德普斯医疗科技有限公司 | A kind of non-woven fabrics and processing method of quick water suction networking partition |
-
2007
- 2007-11-30 CN CNA2007101716220A patent/CN101181648A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103046225A (en) * | 2012-01-19 | 2013-04-17 | 苏州达普生物技术有限公司 | Preparation method of collagen membrane |
| CN103046225B (en) * | 2012-01-19 | 2015-12-02 | 苏州景卓生物技术有限公司 | A kind of preparation method of collagen film |
| CN110129997A (en) * | 2019-06-05 | 2019-08-16 | 安徽迈德普斯医疗科技有限公司 | A kind of non-woven fabrics and processing method of quick water suction networking partition |
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Open date: 20080521 |