CN101181540B - Application of pharmaceutical composition in the preparation of medicine for prostatitis resistance - Google Patents
Application of pharmaceutical composition in the preparation of medicine for prostatitis resistance Download PDFInfo
- Publication number
- CN101181540B CN101181540B CN 200710056354 CN200710056354A CN101181540B CN 101181540 B CN101181540 B CN 101181540B CN 200710056354 CN200710056354 CN 200710056354 CN 200710056354 A CN200710056354 A CN 200710056354A CN 101181540 B CN101181540 B CN 101181540B
- Authority
- CN
- China
- Prior art keywords
- parts
- group
- medicine
- strain
- flos lonicerae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to an application of a medicine compound in the preparation of a medicine for treating prostatitis, belonging to the Chinese medicine field. The invention relates to the Chinese Medicine Preparation for Treating Heat Stranguria, namely a new usage of the medicine compound with the patent No. of 02146487.1. The medicine compound is made from the following Chinese medical raw materials in a formula with a certain weigh proportion: 125 to 375 parts of honeysuckle, 62 to 187 parts of herba scutellariae barbatae, 37 to 112 parts of herba dianthi, 37 to 112 parts of knotgrass, 37 to 112 parts of folium pyrrosiae, 19 to 56 parts of fiveleaf akebia, 37 to 112 parts of semen plantaginis, 37 to 112 of parts common lophatherum herb, 12 to 37 parts of rush and 62 to 187 parts of mistletoe. The invention can be used for treating prostatitis, and the Chinese native medicines in the formula is rich in source, low in cost, convenient in carrying and easy to be accepted by users, so the medicine compound is applicable for the sufferers and has a substantial social and economical benefit.
Description
Technical field
The invention belongs to technical field of Chinese medicines.
Background technology
Jilin Huakang Pharmaceutical Co., Ltd has Chinese invention patent " Chinese medicine preparation for the treatment of pyretic stranguria ", the patent No. is 02146487.1, Granted publication CN1168488C, it is characterized in that, per 1000 doses of raw material of Chinese medicine by following weight proportion are made, 125～375 parts of Flos Loniceraes, 62～187 parts of Herba Scutellariae Barbataes, 37～112 parts of Herba Dianthis, 37～112 parts of Herba Polygoni Avicularis, 37～112 parts of Folium Pyrrosiaes, 19～56 parts of Caulis Akebiaes, 37～112 parts of Semen Plantaginiss, 37～112 parts of Herba Lophatheris, 12～37 parts of Medulla Juncies, 62～187 parts of Herba Taxillis.Its function of preparation of the present invention cures mainly as: heat-clearing and toxic substances removing, dampness removing is treating stranguria.Be applicable to pyretic stranguria (syndrome of dampness-heat in lower jiao) and be equivalent to each disease of acute urinary system infection.
Summary of the invention
The invention provides the application of a kind of pharmaceutical composition in the medicine of preparation resistance prostatitis, " Chinese medicine preparation for the treatment of pyretic stranguria ", the patent No. is the new medicinal usage of 02146487.1 pharmaceutical composition, this pharmaceutical composition is made by the raw material of Chinese medicine of following weight proportion, 125～375 parts of Flos Loniceraes, 62～187 parts of Herba Scutellariae Barbataes, 37～112 parts of Herba Dianthis, 37～112 parts of Herba Polygoni Avicularis, 37～112 parts of Folium Pyrrosiaes, 19～56 parts of Caulis Akebiaes, 37～112 parts of Semen Plantaginiss, 37～112 parts of Herba Lophatheris, 12～37 parts of Medulla Juncies, 62～187 parts of Herba Taxillis.
Among the present invention, this pharmaceutical composition is made by the raw material of Chinese medicine of following weight proportion: 187～312 parts of Flos Loniceraes, 93～156 parts of Herba Scutellariae Barbataes, 56～93 parts of Herba Dianthis, 56～93 parts of Herba Polygoni Avicularis, 56～93 parts of Folium Pyrrosiaes, 28～47 parts of Caulis Akebiaes, 56～93 parts of Semen Plantaginiss, 56～93 parts of Herba Lophatheris, 19～31 parts of Medulla Juncies, 93～156 parts of Herba Taxillis.
Among the present invention, this pharmaceutical composition is made by the raw material of Chinese medicine of following weight proportion: 190～270 parts of Flos Loniceraes, 110～136 parts of Herba Scutellariae Barbataes, 65～85 parts of Herba Dianthis, 65～85 parts of Herba Polygoni Avicularis, 65～85 parts of Folium Pyrrosiaes, 65～85 parts of Caulis Akebiaes, 65～85 parts of Semen Plantaginiss, 65～85 parts of Herba Lophatheris, 12～28 parts of Medulla Juncies, 110～136 parts of Herba Taxillis.
Above medical material weight portion can be made into 1000 doses of medicaments if calculate in grams, as to make 1000 of capsule preparations, 1000 in tablet etc.
Chinese medicine preparation of the present invention, can add the medicine acceptable carrier when being prepared into medicament, described medicine acceptable carrier can be: starch, sucrose, lactose, mannitol, silicon derivative, cellulose family and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Chinese medicine preparation of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, peroral dosage form preferably, as: capsule, tablet, oral liquid, granule, pill, powder, sublimed preparation, unguentum, most preferably capsule.
The present invention has the effect of resistance prostatitis, the Chinese medicine in the pharmaceutical formulation, and its medicine source is abundant, and is cheap, easy to carry, easily accepted by the patient.Be applicable to extensive patients and use, so appreciable social benefit and economic benefit are arranged.Preparation of the present invention is determined usage and dosage according to patient's situation in use, but takes every day three times, each 1～10/or sheet.
Chinese medicine preparation of the present invention can prepare by the following method:
Half amount of Flos Lonicerae is used ethanol extraction, filters, and Recycled ethanol, concentrated for subsequent use, other gets 9 flavors such as Herba Scutellariae Barbatae, Herba Dianthi and decocts with water filtration, and is concentrated for subsequent use.Again half amount, decontamination, the coarse pulverization of extracting honeysuckle, with above two thick pastes for subsequent use, merging, drying is ground into fine powder, sieves, and obtains active component, and this active component can directly be made preparation, also can add the medicine acceptable carrier and make pharmaceutical preparation.
Further verify the present invention below by pharmacodynamic experiment.
Tested medicine
1. medicine of the present invention: hereinafter to be referred as: Flos Lonicerae is secreted scorching clever sheet (Yinhuamiyanling Pian), is provided lot number by Jilin Huakang Pharmaceutical Co., Ltd: 050903.Every crude drug content is 1.875g, and high performance liquid chromatography is carried out quantitative assay, and every of this product contains Flos Lonicerae, with chlorogenic acid (C
10H
18O
9) must not count and be less than 3.60mg.
2. compound method: face the time spent and Flos Lonicerae is secreted scorching clever sheet go to be made into desired concn with distilled water behind the film-coat, the time spent shakes up, 4 ℃ of preservations.
3. dosage is selected: oral 16 of everyone every day of being grown up, every heavy 0.5g, every contains crude drug in whole 1.875g, the people presses 70kg and calculates, be equivalent to 0.428g crude drug/kg, press human body and animal body surface area equivalent dose ratio and calculate, selecting three kinds of dosages of white mice is 15.0g, 7.5g, 3.75g crude drug/kg; Rat is 10.8g, 5.4g, 2.7g crude drug/kg (being equivalent to respectively 4 times of dose,equivalent, 2 times and dose,equivalent), the equal gastric infusion of animal (ig).Route of administration is with clinical consistent.
Animal
1.Wistar rat, closed colony, male and female dual-purpose, animal quality certification SCXK (Ji) 2003--0004.
2. outbreeding system, Kunming kind white mice, closed colony, body weight 18-22g, the animal quality certification number: SCXK (lucky 2003-0004.
Wistar rat and Kunming kind white mice provide by Changchun High-technology Medical Animal Experiment Research Center.
The raising condition
Animal housing is regular grade, 26 ℃ of room temperatures, and relative humidity is 40%, the well-ventilated adopts standard feed to raise, white mice cage tool 26 * 16cm, male and female are divided cage, 5 in every cage.DABAI mouse cage tool 50 * 35cm, male and female are divided cage, 5 animals of every cage
The positive control medicine is selected
1. aspirin (enteric coatel tablets), clinical adult's every day oral this product 40mg, contain aspirin 25mg, face the time spent grinding, selective dose rat 0.2g/kg, white mice 0.3g/kg (determining with reference to the interrelated data test dose), Shijiazhuang Shineway Pharmaceutical Co., Ltd produces, lot number: 20050209.
2 FUSAIMI PIAN, four of clinical adult every days, every contains furosemide 20mg.Tianjin Lisheng Pharmaceutical Co., Ltd. produces, and presses human body and animal body surface area equivalent dose ratio and calculates.Selective dose rat 36mg/kg (5 times of dose,equivalents), lot number: 040811.
3 QIANLIETONG PIAN, it is basic identical that (the accurate word Z44022456 of traditional Chinese medicines number) and Flos Lonicerae are secreted scorching clever sheet therapeutical effect, and route of administration is consistent, so be chosen as positive reference substance.This product is coated tablet, remove behind the sugar-coat aobvious light brown to brown, the heavy 0.34g of substrate, per day for adults 3 times, is pressed human body and animal body surface area equivalent dose ratio and is calculated by each 6, selecting rat dosage is 2.75g/kg, be equivalent to 5 times of dose,equivalents, face time spent desaccharide clothing, grind, be made into desired concn with distilled water, lot number: 04507 is produced in the equal gavage of animal (ig) administration by Guangzhou Zhongyi Medicine Industry Co., Ltd.
4 amoxicillin, everyone 2000mg every day that is grown up, 2 times of dose,equivalent 520mg/kg of test and Selection, Heilungkiang three diligent pharmaceutical Co. Ltds produce lot number: 031010
Instrument
1, JN one A type precision torsion balance (Shanghai Second Balance Factory)
2, SARTORIUS GMBH GOTTINGEN precision balance (precision: 0.00001), Germany
3, L-301 type biological microscope (optical instrument factory, Guangzhou)
4, ultramicrotome LKB2088 (Sweden's manufacturing)
5Leitj1512 paraffin slicing machine (Germany makes)
6SW-CJ-113 standard type clean work station (Suzhou Decontamination Equipment Plant)
7WX-753 type microcirculation microscope (Xuzhou medical optical instrument factory)
8 biomedical image analyzers (CIMA-400)
The multiple star FORTUNE.IMS microbial identification in 9 Shanghai/Analysis of Drug Susceptibility system
10, constant-temperature shaking incubator, HZQ-X100, Taicang experimental facilities factory
11 double superclean benches, SW-CT-ZF type, Chinese Suzhou
12 electric drying oven with forced convections, 101A-2 type, Chinese Shanghai;
13 vacuum freeze driers, MRK, Japan;
14 domestic cold storage refrigerators, BCD-226 type, U.S. Pedicellus et Pericarpium Trapae, Chinese Hefei.
Reagent:
Carrageenin: provided by Shenyang Pharmacy College's department of Chinese materia medica, face the time spent and prepare with normal saline, without lot number; Glacial acetic acid, produced lot number by Beijing North fine chemical company limited: 020730,25% XIAOZHILING ZHUSHEYE, produced lot number: H0031 by Guangzhou Chinese medicine industry company limited; Sodium chloride (analytical pure), the Beijing Chemical Plant produces; Bacto Tryptone, OXOID, Britain; Bacto Yeast Extract, OXOID, Britain; Agar powder, Solution on Chemical Reagents in Shanghai purchasing and supply station chemical reagent work produces, lot number: 77-07-14.Albumen, the East Sea, Shanghai pharmaceutical factory produces.
Bacterial strain: reference culture (American Type Culture Collection ATCC): colon bacillus ATCC44102, Staphylococcus aureus ATCC 26003, shigella flexneri ATCC51571.Identify that by Chinese pharmaceutical biological product institute provides.
Clinical isolates strain (Clinical Isolated Bacterial Strain CIBS): staphylococcus aureus strain CIBS831, staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, deformed rod bacterial strain CIBS1072, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, fungus Candida albicans strain CIBS850 and 3 strain coli strain CIBS872, CIBS614, CIBS853.Staphylococcus aureus, bacterium number: 26003 (Attached Hospital, Changchun Chinese Medicinal College's Bacteriology Room provides).
Data statistical approach:
The test 1～10 result data all with
The t check is adopted in expression, the significance of group difference, and test 10,11 results adopt χ
2Check.
Test method and result:
1, Flos Lonicerae is secreted scorching clever sheet activates thing chronic prostatitis model on xiaozhiling injection impact
Make the nonbacterial prostatitis rat model according to prostate direct injections such as Zhang Shi.Get 50 of Wistar rat, male, body weight 350-400g, 3% chloral hydrate 10ml/kg anaesthetizes through lumbar injection, under aseptic condition, Ventral Midline disinfects in alcohol mentions skin afterwards with tweezers, successively cuts skin, through abdominal cavity, mention bladder and both sides spermatic cord, expose bladder dorsal part prostate dorsal part leaf, inject respectively 25% XIAOZHILING ZHUSHEYE 0.1ml, suture muscles, skin, the iodine disinfection wound.Postoperative was raised 7 days meticulously, in the 7th day rat is divided into 5 groups at random, the 1st, 2,3 groups is that Flos Lonicerae is secreted scorching clever sheet height, in, low dose group, dosage is respectively 10.8g, 5.4g, 2.7g crude drug/kg, the 4th group is QIANLIETONG PIAN positive drug group, and dosage is 2.75g/kg, and the 5th group is model group, other gets 10 and is Normal group, 1-4 organizes according to dosage gastric infusion (ig), and all the other give distilled water, successive administration 30 days, in the last administration after 1 hour, weigh, the sacrificed by decapitation rat, dissection is got prostate and is weighed, then respectively get prostatic half, place the 1ml normal saline, fully mixing is measured leukocyte count and lecithin number in the prostatic fluid in microscopically, all the other prostate are fixed with 10% formalin, do histopathology section procuratorial work.The results are shown in Table 1, table 2.
Table 1 Flos Lonicerae is secreted scorching clever sheet to the impact of rat chronic prostatitis model prostatic fluid
Medication group and model control group be * P＜0.05 relatively
Normal group and model group be ##P＜0.01###P＜0.001 relatively
Prostatic fluid is measured and is shown, model group and Normal group are relatively, leukocyte count obviously raises, and the lecithin number obviously reduces, confirm this model establishment, tested medicine Flos Lonicerae is secreted quantity of leucocyte and the apparent in view minimizing of model control group of scorching clever sheet height, middle dosage group, and the lecithin number has obvious rising, and learning by statistics to process has significant difference P＜0.05.
Table 2 Flos Lonicerae is secreted scorching clever sheet rat chronic prostatitis model is affected the sxemiquantitative classification
Annotate: grade scale:
0 grade: normal prostatic, a matter are without broadening and edema, without cell infiltration, without obviously proliferation of fibrous tissue;
1 grade: body of gland is arranged still neat, a light weight degree broadening, and edema is slighter, and a small amount of cell infiltration and proliferation of fibrous tissue are arranged;
2 grades: body of gland is arranged more neat, a matter moderate broadening, and edema is heavier, and more cell infiltration and proliferation of fibrous tissue are arranged;
3 grades: gland tissue has destruction, the obvious broadening of a matter, and edema is obvious, and visible a large amount of cell infiltration and more obvious proliferation of fibrous tissue have the inflammatory cell of infiltration under the tunicle.
The histopathology observed result:
(1), Normal group: prostate lumen of gland out-of-shape, the glandular epithelium lumen of gland is projecting inward, forms a plurality of pleats and stretches to lumen of gland.Glandular epithelium is column or cube, and epithelial proliferation is inactive, and the nipple of formation is few and lack, visible thin Yihong secretions of having a liking in the lumen of gland.Closely arrange between body of gland, a matter is narrow, and a matter composition obviously is less than body of gland, and a matter includes and a small amount of smooth muscle cell, lymphocyte and fibroblast.Be about 1/3 of body of gland, without obvious inflammatory cell.And marshalling,
(2), model group: body of prostate is arranged loose, and glandular epithelium is column.Glandular epithelium is without obvious hypertrophy, and a matter is obviously widened, edema, visible most lymphocytic infiltrations and fibroblast proliferation.Deposition of fibrous tissue compared with normal matched group is many.Some position deposition of fibrous tissue is than compared with normal matched group showed increased.
(3), positive control drug group: body of gland is arranged more loose, and glandular epithelium is shorter, and glandular epithelium does not form more prominent nipple to lumen of gland without obvious hypertrophy.As seen have a liking on a small quantity Yihong secretions in the lumen of gland.Closely arrange between body of gland.Between the light weight degree widen, the degree of lymphocytic infiltration and fibroblast proliferation obviously alleviates than model group, the interstitial fibers tissue deposition is not obvious.
(4), high dose group: body of gland is arranged and is tending towards the form of Normal group, and glandular epithelium is column or cube, and adenomatosis is not obvious, does not form most prominent nipples to lumen of gland.As seen have a liking on a small quantity Yihong secretions in the lumen of gland.Compare with model group, a matter width obviously narrows down, and edema obviously alleviates, and the inflammatory cell of infiltration is sparse.The interstitial fibers tissue deposition is not obvious.
(5), middle dosage group: glandular epithelium does not form more prominent mastoid process to lumen of gland without obvious hypertrophy, as seen has a liking on a small quantity Yihong secretions in the lumen of gland.Glands morphology and positive controls are close, and a matter widens degree and interstitial edema obviously alleviates, and the inflammatory cell quantity of infiltration reduces, sparse distribution, and the interstitial fibers tissue deposition is not obvious.
(6), low dose group: body of gland is arranged still neat, and glandular epithelium does not form more prominent mastoid process to lumen of gland without obvious hypertrophy, as seen has a liking on a small quantity Yihong secretions in the lumen of gland.Between matter widen with edema and alleviate to some extent than model group, a matter inflammatory cell quantity reduces, visible a small amount of interstitial fibers tissue deposition.
Conclusion:
The model group body of gland is arranged more loose, disorder, and the obvious edema of a matter is widened, as seen most cell infiltration, fibroblast proliferation and deposition of fibrous tissue occur, some position deposition of fibrous tissue showed increased shows that the chronic prostatitis model of foundation is successfully.
Flos Lonicerae is secreted scorching clever sheet height, middle dosage group and positive drug control group, the prostate body of gland is arranged and is tending towards normal, matter is widened between prostate, the edema degree obviously alleviates than model group, between the inflammatory cell that infiltrates of matter obviously reduce, the interstitial fibers tissue deposition obviously alleviates, and wherein the improvement effect of high dose is the most obvious.
2 Flos Lonicerae are secreted scorching clever sheet to the impact of rat nonbacterial prostatitis
Get 60 of rats, body weight 300-350g, after be divided at random 6 groups, 1-3 group is secreted the high, medium and low dosage group of scorching clever sheet for Flos Lonicerae, dosage is respectively 10.8g, 5.4g, 2.7g crude drug/kg, 4 groups of positive medicine QIANLIETONG PIAN groups, dosage is 2.75g/kg, and 5 groups is model control group, and 6 groups is Normal group, 1-4 organizes equal gavage (ig) administration, and model control group and Normal group give with the volume distilled water by the administration high dose.Every day 1 time, continuous 10 days, 1h improves a little by the Dai Shi method after the last administration, every Mus is cut the abdominal cavity with lumbar injection 3% chloral hydrate 10ml/kg anesthesia open under the aseptic condition, injects sterilization 1.5% carrageenin 0.05ml in every Mus prostate, (Normal group injection physiological saline solution), sew up 24h sacrificed by decapitation rat, (row is administered once again) therebetween, getting prostate weighs, after get half prostate in the 1ml normal saline, fully mixing is drawn the prostate suspension with capillary tube, splash in the blood countng chamber, move into microscopically, with the low power lens counting, press record leukocyte count and Lecithin corpusculum number under the leukocyte method mirror; Simultaneously prostatic fluid is inoculated on the common blood agar plate, cultivates 24h for 37 ℃.Inspection has or not colony growth.The residue prostate is fixed with formalin, gets rat prostate and does histopathologic examination.The results are shown in Table 3, table 4.
Table 3 is on the impact of rat nonbacterial prostatitis model prostatic fluid test rating
Medication group and model control group are relatively
*P＜0.05
*P＜0.01
Normal group and model group are relatively
*P＜0.01
* *P＜0.001
The result shows by table 3, Flos Lonicerae is secreted scorching clever sheet 10.8g crude drug/kg, 5.4g crude drug/kg dosage group and rat carrageenan prostatitis model is had obvious inhibition leucocyte hyperplasia and improve the effect of Lecithin corpusculum density, relatively distinguishes P＜0.01, P＜0.05 with model control group.Prostatic fluid is showed no colony growth simultaneously.
Pathological study result shows, Normal group: the prostate lumen of gland is regular, closely arranges between body of gland, and a matter is narrow, without obvious cell infiltration.Model control group: body of prostate is arranged loose, disorder, and a matter is obviously widened, edema, visible most lymphocytic infiltrations and fibroblast proliferation.Deposition of fibrous tissue compared with normal matched group is many.Cell infiltration obvious (success of prostatitis model) under the visible capsule of prostate in some position.Flos Lonicerae is secreted scorching clever sheet 10.8g crude drug/kg dosage group: body of gland is arranged and is tending towards the Normal group degree, and adenomatosis is not obvious.Between the matter width than the obvious constriction of model group, edema is slight, the inflammatory cell of infiltration is sparse.Most of tunicle has no obvious cell infiltration.Flos Lonicerae is secreted scorching clever sheet 5.4g crude drug/kg dosage group: body of gland is arranged more loose, and the matter degree of widening obviously alleviates, and the inflammatory cell quantity that a matter infiltrates reduces, and edema alleviates, visible minority cell infiltration under the capsule of prostate.Positive drug QIANLIETONG PIAN group: Glands morphology and Flos Lonicerae secrete that scorching clever sheet 5.4g crude drug/kg dosage group is close, and the matter degree of widening alleviates than model control group, the inflammatory cell negligible amounts that a matter infiltrates.In sum, Flos Lonicerae is secreted scorching clever sheet to be had prostatitis rat and obviously alleviates effect, especially remarkable with effect of high dosage.
Table 4 Flos Lonicerae is secreted scorching clever sheet rat nonbacterial prostatitis model is affected the sxemiquantitative classification
Annotate: grade scale: (the sxemiquantitative grade scale sees Table 2 grade scales)
The result shows by table 4, and Flos Lonicerae is secreted scorching clever sheet height, middle dosage group all has the effect that alleviates the prostata tissue pathological changes, and the effect of wherein administration high dose group is the most obvious.
3, Flos Lonicerae is secreted scorching clever sheet to the impact of rat bacterial prostatitis due to the escherichia coli
Get 60 of rats, body weight 280-340g, after be divided at random six groups, press the Dai Shi method with 0.1ml colibacillus (1.4 * 10
7/ ml) cause inflammation (method is with 2), cause scorching front 5 days and begin by group shown in the table 4 and dosage grouping administration (ig), model group and Normal group give with the volume distilled water by the administration high dose.Cause the scorching rear administration 3 days that continues every day 1 time, 1h after the last administration, and the sacrificed by decapitation rat is got respectively prostate 50mg under aseptic condition, and in the 1ml normal saline, fully mixing records leukocyte count and Lecithin corpusculum number under the mirror; Simultaneously prostatic fluid is inoculated on the common blood agar plate, cultivates 48h for 37 ℃.The record clump count, each group is got prostate and is fixedly done histopathologic examination with formalin simultaneously.
Table 5 Flos Lonicerae is secreted scorching clever sheet causes rat bacterial prostatitis model prostatic fluid test rating on escherichia coli impact
Administration group and model control group are relatively
*P＜0.05
*P＜0.01
Normal group and model control group are relatively
*P＜0.01
* *P＜0.001
The result shows by table 5, Flos Lonicerae is secreted scorching clever sheet 10.8g crude drug/kg, 5.4g crude drug/kg dosage group causes the rat bacterial prostatitis model to escherichia coli and has obvious minimizing quantity of leucocyte and improve the effect of Lecithin corpusculum density, also can reduce simultaneously the bacterial population in the prostatitis rat prostate, with model control group statistical significance is arranged more all, P＜0.01, P＜0.05.
The impact of 4, the intraperitoneal mouse capillary permeability being increased
Choose 50 of healthy mices, body weight is 18-22g, and male and female half and half are divided into 5 groups at random, 10 of every treated animals.The 1st, 2,3 groups is that Flos Lonicerae is secreted scorching clever sheet height, in, low dose group, dosage is 15.0g respectively, 7.5g, 3.75g crude drug/kg, the 4th group of positive medicine aspirin, dosage is 0.3g/kg, the 5th group is matched group, the continuous gastric infusion of animal 5 days, tail vein injection 0.5% azovan blue solution 0.01mL/g behind last administration 1h, lumbar injection 0.6% acetic acid normal saline 0.2ml/ only takes off cervical vertebra and puts to death white mice behind the 20min immediately, dabs abdominal part 1min, cutting the abdominal cavity open washes repeatedly with an amount of normal saline, collect eluate, filter paper filters, and adjusts volume to 6mL with normal saline, with the centrifugal 15min of centrifuge (3000rPm), get supernatant (wavelength 590nm) in 752 type spectrophotometers and measure the OD value, difference of relatively respectively organizing, and directly calculate suppression ratio.The results are shown in Table 6.
Table 6 Flos Lonicerae is secreted scorching clever sheet to the impact of intraperitoneal mouse capillary permeability (X ± SD)
Compare with matched group
*P＜0.05
*P＜0.01
Table 6 result shows, Flos Lonicerae secretes that scorching clever sheet is high, middle dosage Dichlorodiphenyl Acetate cause the intraperitoneal mouse capillary permeability increase and have obvious inhibitory action (with matched group relatively, P＜0.05), but its effect is not as positive drug (P＜0.01)
5, on the impact of rat thoracic cavity leukoplania
Get 50 of rat, male and female half and half are divided into 5 groups at random, 10 of every treated animals, the 1-3 group is secreted the high, medium and low dosage group of scorching clever sheet for Flos Lonicerae, and dosage is respectively 10.8g, 5.4g, 2.7g/kg, 4 groups of positive medicine aspirin groups, dosage is 0.2g/kg, 5 groups is matched group, and 1-4 organizes according to dosage gavage (ig) administration, every day 1 time during experiment, continuous 5 days, matched group gave with the volume distilled water by the administration high dose group.Behind last administration 1h, with rat ether light anaesthesia, face upward the position and be fixed on the operating-table.Inject 0.5% carrageenin 0.5ml in Rat Right pleurobranch chamber, sacrificed by decapitation rat after 5 hours is opened the thoracic cavity at the tabula periphery, and with syringe sucking-off pleural fluid, record seepage discharge and leukocyte count the results are shown in Table 7.
Table 7 Flos Lonicerae is secreted scorching clever sheet to the impact of rat thoracic cavity leukoplania (X ± SD)
Compare with matched group
*P＜0.05
*P＜0.01
As shown in Table 7, Flos Lonicerae is secreted scorching clever sheet height, the equal rat of middle dosage thoracic cavity leukoplania has certain inhibitory action, but its effect is weaker than positive control drug aspirin.
6, on the impact of rat chondrus ocellatus Holmes colloidality foot swelling
Get 50 of rat, male, be divided at random five groups, 10 of every treated animals, the 1-3 group is secreted the high, medium and low dosage group of scorching clever sheet for Flos Lonicerae, and dosage is respectively 10.8g, 5.4g, 2.7g/kg, 4 groups of positive medicine aspirin groups, dosage is 0.2g/kg, 5 groups is matched group, and 1-4 organizes according to dosage gavage (ig) administration, every day 1 time during experiment, continuous 7 days, matched group gave with the volume distilled water by the administration high dose group.Measure the left back ankle of rat joint girth with self-control arrowband chi.Behind last administration 30min, respectively at the right back sufficient subcutaneous injection 1% carrageenin suspension 0.1ml/ of rat only.Per hour survey right back ankle joint girth 1 time, altogether 6h.From as contrast, take the difference of left and right sides metapedes ankle joint girth as inflammation swelling (mm), the results are shown in Table 8 with the left back foot of every Mus
Table 8 Flos Lonicerae is secreted scorching clever sheet to the impact of rat chondrus ocellatus Holmes colloidality foot swelling (X ± SD)
Compare with matched group
*P＜0.05
*P＜0.01
* *P＜0.001
The result shows by table 8, and Flos Lonicerae is secreted scorching clever sheet 10.8g/kg group, and foot swelling has obvious inhibitory action to 1～5h rat chondrus ocellatus Holmes colloidality; Flos Lonicerae secretes scorching clever sheet 10.8g crude drug/kg group and the 5.4g crude drug/kg group 1～2h has inhibitory action, but its effect illustrates that not as the positive drug aspirin this product has certain antiinflammatory action.
7, on the impact of white mice writhing response incidence rate
Get 50 of white mice, male and female half and half are divided into five groups at random by dosage shown in the table 9,10 of every treated animals, animal ig administration, every day 1 time, continuous 5 days (positive drug administration on the same day), matched group is given with the volume distilled water, 1h after the last administration, every Mus is begun behind the 5min to observe and the interior white mice writhing response number of times of record 10min by lumbar injection 0.6% acetum 0.1ml/10g (body weight), the results are shown in Table 9.
Computing formula:
Table 9 Flos Lonicerae is secreted scorching clever sheet to the impact of white mice writhing response (X ± SD)
Compare * * * P＜0.001 with matched group
The result shows by table 9, and Flos Lonicerae is secreted high, medium and low three dosage of scorching clever sheet and all had obvious analgesic activity, and suppression ratio is respectively 47.21%, 42.49%, 51.07%.But effect is not as positive drug aspirin group.
8, to the diuresis of rat
Get 50 of rat, be divided at random 5 groups, the 1st, 2,3 group is that Flos Lonicerae is secreted the high, medium and low dosage group of scorching clever sheet, the 4th group of positive medicine FUSAIMI PIAN group, and the 5th group is matched group.Each administration group is gastric infusion according to dosage, continuous 5 days, matched group is given with the volume distilled water, after the last administration 0.5 hour, each Mus was pressed the normal saline lumbar injection of 2.5ml/100g, and gently depressed abdominal part and make bladder emptying (test fasting the previous day is water 18hr not only), immediately rat is put into metabolic cage, a cage is put 1 of animal, collects the urine amount totally 3 times of each treated animal after 60 minutes, each 60 minutes.Relatively, take statistics to learn and process between the average of each the group urine amount of rat in the corresponding time organized, the results are shown in Table 10.
Table 10 Flos Lonicerae is secreted scorching clever sheet to the impact of Urine of Rats amount (X ± SD)
Compare with matched group
*P＜0.05
* *P＜0.001
By upper table result show Flos Lonicerae secrete scorching clever sheet high dose group to the diuresis of white mice significantly (
*P＜0.05), but the effect not as the positive drug group (
* *P＜0.005), middle dosage group and low dose group action effect are not obvious.
9, Flos Lonicerae is secreted scorching clever sheet to the impact of rat granuloma hamartoplasia
Get 50 of rats, every Mus is with lumbar injection 3% chloral hydrate 10ml/kg anesthesia anesthesia, under aseptic condition, with strange section iodine disinfection about rat, after 75% ethanol took off iodine, each cut the long osculum of 1-1.5cm, with ophthalmic tweezers 20mg (sterilization) cotton balls is imbedded subcutaneous, skin suture immediately, animal was stablized one day, be divided at random 5,10 every group, press dosage shown in the table 11, the equal ig administration of animal, every day 1 time, continuous 7 days, matched group was given with the volume distilled water.In the 7th day rat is put to death, open former otch, take out cotton balls, reject fatty tissue, put 70 ℃ of oven dry of baking oven, weigh.The results are shown in Table 11.
Computing formula:
Granuloma weight=(two granuloma weight/2)-20mg
Table 11 Flos Lonicerae is secreted scorching clever sheet to the impact of rat granuloma hamartoplasia
Compare * P＜0.05**P＜0.01***P＜0.001 with matched group
As shown in Table 11, Flos Lonicerae is secreted scorching clever sheet 10.8g crude drug/kg, two kinds of dosage of 5.4g crude drug/kg have obvious inhibitory action to cott on pellet-induced granuloma formation, wherein 10.8g crude drug/kg group is learned by statistics and is processed P＜0.01, but effect is not as positive control drug aspirin.
10, Flos Lonicerae is secreted scorching clever sheet to the protective effect of staphylococcus aureus In vivo infection white mice
With the staphylococcus aureus dried frozen aquatic products, be inoculated in the fluid medium after the recovery, in incubator, cultivated 18 hours for 37 ℃, use normal saline with 10 times of serial dilutions after taking out, add simultaneously the bacteria suspension that 5% concentration gastron (to strengthen virulence) is made different gradients.Through trial test, lumbar injection staphylococcus aureus minimum lethal dose (MLD) is 10 under this experimental condition
-1Formal test, get 75 of mices, male and female half and half, body weight 18-20g, be divided at random 5 groups, every group 15,1st, 2,3 groups is that Flos Lonicerae is secreted scorching clever sheet group, and dosage is respectively 31.2g, 15.6g, 7.8g crude drug/kg (be equivalent to respectively mice dose,equivalent 8 times, 4 times, 2 times), the 4th group of positive medicine Amoxicillin Capsules group, dosage is 0.52g/kg (being equivalent to 2 times of dose,equivalents), the 5th group be Normal group to distilled water 10ml/kg, every day, the ig administration was 2 times, the upper and lower noon each once, successive administration 5 days was in administration pneumoretroperitoneum injection 10 in the 5th day
-1The staphylococcus aureus suspension, every 0.5ml, and continue administration, and observe 24h dead animal number, calculate mortality rate and carry out x
2Statistical procedures the results are shown in Table 12.
Table 12 Flos Lonicerae is secreted scorching clever sheet to the protective effect of staphylococcus aureus In vivo infection mice
Table 12 is the result show; Flos Lonicerae is secreted scorching clever sheet 31.2g crude drug/kg dosage group and staphylococcus aureus is caused the mice In vivo infection relatively P＜0.05 of significant protective effect and matched group is arranged; other two dosage also have inhibition trend in various degree, but statistical procedures is without explicitly difference.Illustrate that this product has obvious bacteriostatic activity.
11, Flos Lonicerae is secreted scorching clever sheet to the protective effect of escherichia coli In vivo infection white mice
With the escherichia coli dried frozen aquatic products, be inoculated in the fluid medium after the recovery, in incubator, cultivated 18 hours for 37 ℃, use normal saline with 10 times of serial dilutions after taking out, add simultaneously the bacteria suspension that 5% concentration gastron (to strengthen virulence) is made different gradients.Through trial test, lumbar injection staphylococcus aureus minimum lethal dose (MLD) is bacterium stock solution under this experimental condition.Formal test, get 75 of mices, male and female half and half, body weight 18-20g, be divided at random 5 groups, every group 15,1st, 2,3 groups is that Flos Lonicerae is secreted scorching clever sheet group, and dosage is respectively 31.2g, 15.6g, 7.8g crude drug/kg (be equivalent to respectively mice dose,equivalent 8 times, 4 times, 2 times), the 4th group of positive medicine Amoxicillin Capsules group, dosage is 0.52g/kg (being equivalent to 2 times of dose,equivalents), the 5th group be Normal group to distilled water 10ml/kg, every day, the ig administration was 2 times, the upper and lower noon each once, successive administration 5 days was in administration pneumoretroperitoneum injection 10 in the 5th day
-1The staphylococcus aureus suspension, every 0.5ml, and continue administration, and observe 24h dead animal number, calculate mortality rate and carry out x
2Statistical procedures the results are shown in Table 13.
Table 13 Flos Lonicerae is secreted scorching clever sheet to the protective effect of escherichia coli In vivo infection mice
Table 13 is the result show; Flos Lonicerae is secreted scorching clever sheet 31.2g crude drug/kg dosage group and staphylococcus aureus is caused the mice In vivo infection relatively P＜0.01 of significant protective effect and matched group is arranged; other two dosage also have inhibition trend in various degree, but statistical procedures is without explicitly difference.Illustrate that this product has obvious bacteriostatic activity.
12, Flos Lonicerae is secreted the external bacteriostatic test of scorching clever sheet and bactericidal assay
1. minimum inhibitory concentration (MIC) is measured: with Flos Lonicerae secrete scorching clever sheet medicated powder with the LB fluid medium be mixed with respectively 300,250,200,150,100,80,60,40,20,10mgml
-1Experiment is established 21 groups, and the 1-10 group adds respectively above-mentioned 300mgml
-1-10mgml
-1The medicinal liquid of 10 concentration, every group of 3 parallel control pipes; The 11-20 group does not add bacterium liquid group for only adding the clever sheet medicinal liquid of the secret inflammation of above-mentioned same concentrations Flos Lonicerae, as the medicine matched group whether the observation medicine pollutes, every group of 3 parallel control pipes; Do not secrete the matched group that scorching clever sheet medicinal liquid only adds antibacterial for not adding Flos Lonicerae for 21 groups, establish simultaneously 3 parallel control pipes.To be coated with flat board behind the suitable multiple of inoculated bacteria dilution, overnight incubation in 37 ℃ of incubators, colony counting is also taken pictures.With each the group developmental tube put cultivate 16～18 hours in 37 ℃ of constant-temperature shaking incubators after, above each drug component of lowest concentration of drug that observation is had no bacterial growth (comparing with the medicinal liquid matched group) is not coated with flat board, overnight incubation in 37 ℃ of incubators with the suitable multiple of liquid in pipe dilution.Colony counting is also taken pictures.Bacteriostasis rate reaches 99.99% lowest concentration of drug and is minimum inhibitory concentration (MIC).Measurement result table 14 and table 15.
2. minimum bactericidal concentration (MBC) is measured: with Flos Lonicerae secrete scorching clever sheet medicated powder with the LB fluid medium be mixed with respectively 300,250,200,150,100,80,60,40,20,10mgml
-1Experiment is established 21 groups, and the 1-10 group adds respectively above-mentioned 300mgml
-1-10mgml
-1The medicinal liquid of 10 concentration, every group of 3 parallel control pipes; The 11-20 group does not add bacterium liquid group for only adding the clever sheet medicinal liquid of the secret inflammation of above-mentioned same concentrations Flos Lonicerae, as the medicine matched group whether the observation medicine pollutes, every group of 3 parallel control pipes; Do not secrete the matched group that scorching clever sheet medicinal liquid only adds antibacterial for not adding Flos Lonicerae for 21 groups, establish simultaneously 3 parallel control pipes.Behind inoculation bacterium liquid, put in 37 ℃ of constant-temperature shaking incubators and cultivated 16～18 hours.Observe each above developmental tube culture of minimal inhibitory concentration of drug (MIC), draw respectively 0.1ml, move on the ordinary flat agar culture medium, smoothen bacterium liquid with Glass rod, put 37 ℃ of overnight incubation, in the plating medium, counting is less than 5 bacterium colony persons as the minimum bactericidal concentration (MBC) of this medicine.Measurement result sees Table 15
3. results and analysis: patina is secreted scorching clever sheet to colon bacillus strain ATCC44102, staphylococcus aureus strain ATCC26003, shigella flexneri strain ATCC51571; Clinical SEPARATION OF GOLD Staphylococcus aureus strain CIBS831, staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, deformed rod bacterial strain CIBS1072, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, fungus Candida albicans strain CIBS850 and 3 strain coli strain CIBS872, CIBS614, the experiment of CIBS853 In Vitro Bacteriostasis all have preferably antibacterial and bactericidal effect.Patina is secreted scorching clever sheet the MIC of coli strain CIBS872, CIBS614, CIBS853, staphylococcus epidermidis strain CIBS 2972, deformed rod bacterial strain CIBS 1072, enterococcus faecalis strain CIBS 859, colon bacillus strain ATCC44102, staphylococcus aureus strain ATCC26003 is 80mgml
-1The MIC of staphylococcus aureus strain CIBS 831 is 100mgml
-1The MIC of klebsiella spp strain CIBS 861 is 150mgml
-1The MIC of staphylococcus saprophyticus strain CIBS 3586 and bacillus pyocyaneus strain CIBS 868 is 60mgml
-1The MIC of fungus candida albicans strain CIBS 861 and shigella flexneri ATCC51571 is 40mgml
-1The MBC of staphylococcus aureus strain CIBS 831 and colon bacillus strain ATCC44102 is 300mgml
-1The MBC of coli strain CIBS872, deformed rod bacterial strain CIBS1072 and Staphylococcus aureus ATCC 26003 is 250mgml
-1The MBC of coli strain CIBS614, CIBS853, staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, fungus candida albicans strain CIBS861 is 200mgml
-1The MBC of shigella flexneri ATCC51571 is 150mgml
-1, the MBC of bacillus pyocyaneus strain CIBS868 is 80mgml
-1Can find out from top result, Flos Lonicerae is secreted scorching clever sheet to e. coli strains CIBS872, CIBS614, CIBS853, and staphylococcus epidermidis strain CIBS2972, deformed rod bacterial strain CIBS1072, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, fungus candida albicans strain CIBS850 and colon bacillus strain ATCC44102, staphylococcus aureus strain ATCC26003, shigella flexneri strain ATCC51571 all have certain bacteriostasis; Flos Lonicerae is secreted scorching clever sheet to e. coli strains CIBS614, CIBS853, and staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, shigella flexneri strain ATCC5 1571 and fungus candida albicans strain CIBS861 all have certain bactericidal action.
Above-mentioned result of the test shows that Flos Lonicerae is secreted scorching clever sheet to reference culture antibacterial colon bacillus strain ATCC44102, staphylococcus aureus strain ATCC26003, shigella flexneri strain ATCC51571; Clinical SEPARATION OF GOLD Staphylococcus aureus strain CIBS831, staphylococcus epidermidis strain CIBS2972, staphylococcus saprophyticus strain CIBS3586, deformed rod bacterial strain CIBS1072, klebsiella spp strain CIBS861, enterococcus faecalis strain CIBS859, bacillus pyocyaneus strain CIBS868, fungus Candida albicans strain CIBS850 and 3 strain coli strain CIBS872, CIBS614, CIBS853 have certain inhibitory action.
[0151]?
[0153]Conclusion
Show that by the animal experiment result of study Flos Lonicerae is secreted scorching clever sheet various dose and all the chronic prostatitis animal model had obvious therapeutical effect.Simultaneously bacillary, two kinds of acute prostatitis models of non-bacterial also there are obvious inhibitory action, prove that this product has obvious therapeutic action to prostatitis.Test has also confirmed the antiphlogistic effects of this medicine, and the experimenter thinks that its anti-inflammatory activity may be the pharmacological basis of this product.This product has obvious inhibitory action to intraperitoneal mouse capillary permeability, the colloidality foot swelling of rat chondrus ocellatus Holmes and rat cott on pellet-induced granuloma formation.White mice In vivo infection staphylococcus aureus and escherichia coli are had significant protective effect, and In Vitro Bacteriostasis, bactericidal assay result show: to the strain of reference culture antibacterial colon bacillus, staphylococcus aureus strain, shigella flexneri strain; The bacterial strains such as the staphylococcus aureus strain of clinical separation, staphylococcus epidermidis strain, staphylococcus saprophyticus strain all have certain antibacterial and bactericidal action.Show that this product has antiinflammatory, antibacterial, bactericidal action; In addition, this product also has the effects such as obvious diuresis and minimizing white mice writhing response incidence rate.
In sum, Flos Lonicerae is secreted scorching clever sheet and is had and treat preferably acute and chronic prostatitis (new indication) effect, and this medicine also has antiinflammatory, pain relieving, improves many-sided pharmacological actions such as microcirculation and diuresis simultaneously.
The specific embodiment
Embodiment 1: tablet
Prescription
Flos Lonicerae 270g, Herba Scutellariae Barbatae 136, Herba Dianthi 85g Herba Polygoni Avicularis 85g, Folium Pyrrosiae 85g, Caulis Akebiae 85g, Semen Plantaginis 85g, Herba Lophatheri 85g, Medulla Junci 28g, Herba Taxilli 136g makes 1000.
Method for making
80% of Flos Lonicerae, with 70% alcohol reflux 2 times, each 3 hours, add amount of alcohol and be followed successively by 8 times, 6 times of crude drug amount, merge extractive liquid,, filter, Recycled ethanol, for subsequent use when being concentrated into relative density and being 1.2～1.5 (60 ℃), other gets 9 flavors such as Herba Scutellariae Barbatae, Herba Dianthi and decocts with water and extract 3 times, and decocting time is followed successively by 1,5 hours, 1.5 hours, 1 hour, amount of water is followed successively by 10 times, 8 times, 6 times merge extractive liquid, of crude drug amount, filters, and is for subsequent use when being concentrated into relative density and being 1.10～1.35 (60 ℃).Again half amount, decontamination, the coarse pulverization of extracting honeysuckle merges with above two thick pastes for subsequent use, and drying is ground into fine powder, sieve, and granulation, drying is pressed into 1000, sugar coating, and get final product.
Embodiment 2: capsule
Prescription
Flos Lonicerae 270g, Herba Scutellariae Barbatae 136g, Herba Dianthi 85g, Herba Polygoni Avicularis 85g, Folium Pyrrosiae 85g, Caulis Akebiae 85g, Semen Plantaginis 85g, Herba Lophatheri 85g, Medulla Junci 28g, Herba Taxilli 136g makes 1000 capsules.
Method for making
80% of Flos Lonicerae, with 70% alcohol reflux 2 times, each 3 hours, add amount of alcohol and be followed successively by 8 times, 6 times of crude drug amount, merge extractive liquid,, filter, Recycled ethanol, for subsequent use when being concentrated into relative density and being 1.2～1.5 (60 ℃), other gets Herba Scutellariae Barbatae, Herba Dianthi etc. 9 flavor and decocts with water and extract 3 times, and decocting time is followed successively by 1.5 hours, 1.5 hours, 1 hour, and amount of water is followed successively by 10 times, 8 times, 6 times merge extractive liquid, of crude drug amount, filter, for subsequent use when being concentrated into relative density and being 1.10～1.35 (60 ℃).Again half amount, decontamination, the coarse pulverization of extracting honeysuckle merges with above two thick pastes for subsequent use, and drying is ground into fine powder, sieves granulation, drying, 1000 capsules of packing into and get final product.
Claims (1)
1. the application of pharmaceutical composition in the medicine of preparation resistance prostatitis, this pharmaceutical composition is made by the raw material of Chinese medicine of following weight proportion, 125 ~ 375 parts of Flos Loniceraes, 62 ~ 187 parts of Herba Scutellariae Barbataes, 37 ~ 112 parts of Herba Dianthis, 37 ~ 112 parts of Herba Polygoni Avicularis, 37 ~ 112 parts of Folium Pyrrosiaes, 19 ~ 56 parts of Caulis Akebiaes, 37 ~ 112 parts of Semen Plantaginiss, 37 ~ 112 parts of Herba Lophatheris, 12 ~ 37 parts of Medulla Juncies, 62 ~ 187 parts of Herba Taxillis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710056354 CN101181540B (en) | 2007-11-27 | 2007-11-27 | Application of pharmaceutical composition in the preparation of medicine for prostatitis resistance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710056354 CN101181540B (en) | 2007-11-27 | 2007-11-27 | Application of pharmaceutical composition in the preparation of medicine for prostatitis resistance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101181540A CN101181540A (en) | 2008-05-21 |
| CN101181540B true CN101181540B (en) | 2013-01-23 |
Family
ID=39447053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710056354 Active CN101181540B (en) | 2007-11-27 | 2007-11-27 | Application of pharmaceutical composition in the preparation of medicine for prostatitis resistance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101181540B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101342216B (en) * | 2008-07-23 | 2012-07-18 | 孙庆敏 | Medicine for curing prostatitis and preparation method thereof |
| CN102552784B (en) * | 2012-02-05 | 2013-06-19 | 王永刚 | Preparation method of traditional Chinese medicine for treating prostatitis caused by fire excess from yin deficiency |
| CN104042957A (en) * | 2014-06-25 | 2014-09-17 | 姜丽莎 | Traditional Chinese medicine (TCM) composition for treating prostatitis |
| CN112043808A (en) * | 2020-10-27 | 2020-12-08 | 禹州济德医院 | Traditional Chinese medicine preparation for treating prostatitis and preparation method thereof |
| CN114569680B (en) * | 2022-04-11 | 2023-03-24 | 吉林华康药业股份有限公司 | Composition for treating heat stranguria and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1404868A (en) * | 2002-11-12 | 2003-03-26 | 吉林华康药业股份有限公司 | Chinese medicinal preparation for treating pyretic stranguria |
-
2007
- 2007-11-27 CN CN 200710056354 patent/CN101181540B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1404868A (en) * | 2002-11-12 | 2003-03-26 | 吉林华康药业股份有限公司 | Chinese medicinal preparation for treating pyretic stranguria |
Non-Patent Citations (4)
| Title |
|---|
| 中国性学会.中国新世纪性与健康展望 1.中国人口出版社,2001,274-275. |
| 中国性学会.中国新世纪性与健康展望 1.中国人口出版社,2001,274-275. * |
| 国家药典委员会编.国家药品标准 新药转正标准 第50册 1.人民卫生出版社,2006,50-4. |
| 国家药典委员会编.国家药品标准 新药转正标准 第50册 1.人民卫生出版社,2006,50-4. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101181540A (en) | 2008-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102114044A (en) | Artificially processed bear bile powder and preparation method thereof | |
| CN1762967B (en) | Enoxolone derivative, preparation method and uses | |
| CN101181540B (en) | Application of pharmaceutical composition in the preparation of medicine for prostatitis resistance | |
| CN101890030B (en) | Composite capable of preventing bacteria, viruses, oxidation and pigment deposition | |
| CN101698066B (en) | Chinese medicine preparation capable of diminishing inflammation, relieving pains, resisting bacteria, expelling stone and benefiting gallbladder, and preparation method and applications thereof | |
| CN101664446A (en) | Relinging extractum and preparation method and application | |
| CN100353953C (en) | Traditional Chinese medicina preparation for treating oral cavity, throat disease and its preparation method | |
| CN103494928B (en) | Preparation method for compound houttuynia cordata preparation | |
| CN101314004A (en) | Medicament for treating gynaecologic urinary system infection contamination and preparation method thereof | |
| CN1709302A (en) | Chinese medicine formulation for treating chronic pelvic inflammation and its preparing method | |
| CN102302505B (en) | Medicinal composition for treating pelvic inflammatory disease and application thereof | |
| CN101229301B (en) | Chinese traditional medicine compounds for treating urinary system infection and prostatitis | |
| CN101347588B (en) | Medicament for treating gynecology disease and preparation thereof | |
| CN105012826A (en) | Alpinia oxyphylla leaf extract and preparation method and application thereof | |
| CN101455715B (en) | Suppository for treating gynecologic diseases and preparation method thereof | |
| CN103622955A (en) | Application of ellagic acid to preparation of drugs for treating and preventing fungal infection of human body | |
| CN100563677C (en) | A kind of moistening and cleaning throat Chinese medicine preparation | |
| CN109470788A (en) | A kind of method of quality control of FUKE QIANJIN PIAN | |
| CN109364148A (en) | A kind of FUKE QIANJIN PIAN and preparation method thereof | |
| CN101816762A (en) | Medicinal preparation for treating colpitis and preparation method thereof | |
| CN102240328B (en) | Traditional Chinese medicine for treating cold and preparation method thereof | |
| CN105232759A (en) | Pharmaceutical composition for rhinitis treatment and preparation method thereof | |
| CN101066410B (en) | Chinese medicine preparation for treating women's chronic pelvic inflammation and its preparation process | |
| CN100486597C (en) | A pharmaceutical composition made from Chinese traditional medicine for treating snuffle, headache and preparation method thereof | |
| CN100406030C (en) | Chinese medicinal preparation for treating ulcerative stomatitis and method for preparing same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |