CN101177663A - DNA separating micro-fluidic chip - Google Patents
DNA separating micro-fluidic chip Download PDFInfo
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- CN101177663A CN101177663A CNA2007100562738A CN200710056273A CN101177663A CN 101177663 A CN101177663 A CN 101177663A CN A2007100562738 A CNA2007100562738 A CN A2007100562738A CN 200710056273 A CN200710056273 A CN 200710056273A CN 101177663 A CN101177663 A CN 101177663A
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- dna
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- hyperbolic line
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Abstract
The invention relates to a novel structure of a micro-fluidic chip used for DNA separation, pertaining to the field of micro-fluidic chip design for separating DNA or large molecule, etc. The structure comprises a sampling inlet tank (1), an entrance channel (2), a hyperbola micro-shrinkage structure (4), a slit channel (5), a sudden enlarging mouth (6), an outlet channel (7) and a waste liquor (8), which are communicated with each other in sequence; a cylinder-shaped barrier (3) is positioned inside the entrance channel (2) in front of the hyperbola micro-shrinkage structure (4); the cylinder-shaped barrier (3) is positioned on a central line of a micro-fluidic channel. By adopting the cylinder-shaped barrier as a pre-tensile structure, the radius of the cylinder-shaped barrier and the distance between the circle center and the hyperbola micro-shrinkage structure are adjustable according to the real requirements, so as to realize the tensile and the separation of a DNA-chain with different lengths and different original structures. The invention can be driven by flow field and electric field, and the object of the tensile and the separation can be spread to any polymer with a certain universal applicability.
Description
Technical field
The present invention relates to a kind of new texture of isolating micro-fluidic chip design fields such as DNA or biomacromolecule, particularly be specifically related to a kind of preposition right cylinder and hyperbolic line and shrink the coupling micro-fluidic structure.
Background technology
In the enforcement of the Human Genome Project, in order to draw gene mapping, the DNA that need stretch to greatest extent is to improve the order-checking precision.Various structures by micro-fluidic chip can make DNA chain generation distortion to a certain degree, thereby realize stretching or isolating purpose.
The flow passage structure of present DNA separating micro-fluidic chip is a straight channel, the DNA type of drive of this structure is single, only be suitable for adopting electric field driven, and in order to reach certain Di Bola said conditions, need to increase extra electric field intensity, excessive strength of electric field causes the distortion and the damage of micro-fluidic chip easily.And this structure can't effectively stretch to the DNA chain that twines configuration.
Summary of the invention
The objective of the invention is deficiency, a kind of DNA separating micro-fluidic chip with novel preposition right cylinder and hyperbolic line contraction coupling micro-fluidic structure is provided at existing microfluidic chip structure.
Above-mentioned purpose of the present invention is such realization, and accompanying drawings is as follows:
A kind of DNA separating micro-fluidic chip, it comprises the little contraction structure 4 of sample inlet pool 1, entrance channel 2, hyperbolic line, slit conduit 5, the unexpected amplification mouth 6 that is communicated with successively, outlet flow 7, waste liquid pool 8, be positioned at the little contraction structure 4 of hyperbolic line entrance channel 2 before and be provided with cylindrical obstacle 3, cylindrical obstacle 3 places on the fluid channel medullary ray.
The length l of described entrance channel 2
1=1.5～10mm, the width w of entrance channel 2
1=30～200 μ m, the length l of the little contraction structure 4 of hyperbolic line
c=10～180 μ m, the length l of slit conduit 5
2=0.01～1.54mm, the width w of slit conduit 5
2=0.01～3.8 μ m, the length l of outlet flow 7
3=1.5～10mm, the width w of outlet flow 7
3=30～200 μ m.
The equation of the little contraction structure 4 of described hyperbolic line is: the intersection point with fluid channel medullary ray and hyperbolic line ingress is a true origin,
Wherein, coefficient c=w
2l
c/ (2-2w
2/ w
1).
The radius of described preposition cylindrical obstacle 3 can be adjusted as required, change in radius scope 5 μ m～75 μ m.
The position of described preposition cylindrical obstacle 3 can be adjusted as required, and the variable in distance scope of the little contraction structure of its width between centers hyperbolic line 4 ingress is 10 μ m～50 μ m.
Described unexpected amplification mouth 6 is a structure of right angle tyoe.
The present invention is that the DNA chain enters micro-fluidic chip from sample inlet pool by under flow field or electrical forces driving effect, through entrance channel, contacts cylindrical obstacle preceding half when surperficial, and the DNA chain carries out stretching to a certain degree along cylindrical obstacle surface; When the DNA chain moves to back half when surface of cylindrical obstacle gradually, the DNA chain has contraction to a certain degree again, passed through pre-tension deformation after, DNA arrives slit conduit along the little contraction structure of hyperbola, enter outlet flow through the mouth that increases suddenly, flow into waste liquid pool at last.
The present invention adopts cylindrical obstacle as the preliminary draft structure, can adjust the distance etc. of the little contraction structure of the relative hyperbolic line in radius size, the center of circle of cylindrical obstacle as required, with the stretching and the separation of the DNA chain of realizing different lengths, different initial configuration.Structure of the present invention not only can adopt the flow field to drive but also can adopt electric field driven, and stretching and separate object can expand to any polymkeric substance, have certain universality.
Description of drawings
What Fig. 1 represented is that preposition right cylinder and hyperbolic line shrink coupling micro-fluidic structure synoptic diagram.
What Fig. 2 represented is the dynamic changing process mimic diagram of DNA chain through cylindrical obstacle.
What Fig. 3 represented is the stretching and contraction main shaft synoptic diagram of DNA chain through cylindrical obstacle.Among the figure, what the grey solid line was represented is the stretching main shaft of DNA, and solid black lines is represented the contraction main shaft of DNA.
What Fig. 4 represented is that initial configuration is to twine the dynamic changing process mimic diagram of the DNA chain of configuration through single hyperbolic configuration.
What Fig. 5 represented is that initial configuration is to twine the dynamic changing process mimic diagram of the DNA chain of configuration through preposition right cylinder and hyperbolic line contraction coupling micro-fluidic structure.
What Fig. 6 represented is 30 groups of not comparison diagrams of DNA average extensibility in single hyperbolic configuration and preposition right cylinder and hyperbolic line contraction coupling micro-fluidic structure of isomorphism type.
Embodiment
Further specify particular content of the present invention below in conjunction with the accompanying drawing illustrated embodiment:
Consult Fig. 1: the present invention includes: sample inlet pool 1, entrance channel 2, preposition cylindrical obstacle 3, the little contraction structure 4 of hyperbolic line, slit conduit 5, unexpected amplification mouth 6, outlet flow 7, waste liquid pool 8.
The setting channel size is as follows: the length l of entrance channel 2
1=1.5mm, the width w of entrance channel 2
1=200 μ m, the length l of the little contraction structure 4 of hyperbolic line
c=80 μ m, the length l of slit conduit 5
2=1.52mm, the width w of slit conduit 5
2=3.8 μ m, the length l of outlet flow 7
3=1.5mm, the width w of outlet flow 7
2=200 μ m, coefficient c=w
2l
c/ (2-2w
2/ w
1)=155 μ m
2
Under flow field or electrical forces driving effect, the DNA chain enters micro-fluidic chip from sample inlet pool 1, through entrance channel 2, arrive the surface 3 of cylindrical obstacle, preceding half zone at right cylinder obstacle 3, the DNA chain presents tensile state gradually, from original curling type structural changes to the stretching deployed configuration; In half zone, cylindrical back, the DNA chain presents the state that shrinks gradually, changes to curled configuration heavily again from the stretching configuration.The dynamic changing process mimic diagram of contraction-stretchings of DNA chain during-shrink again through cylindrical obstacle as shown in Figure 2, stretch with the contraction main shaft as shown in Figure 3.After having passed through pre-tension deformation, DNA arrives slit conduit along the little contraction structure of hyperbola, enters outlet flow through the mouth that increases suddenly, flows into waste liquid pool at last.
Initial configuration for the DNA chain that twines configuration through the dynamic changing process mimic diagram of single hyperbolic configuration as shown in Figure 4; The dynamic changing process mimic diagram that initial configuration is shunk the coupling micro-fluidic structure through preposition right cylinder and hyperbolic line for the DNA chain that twines configuration as shown in Figure 5.As can be seen, initial configuration is very little for DNA chain extensibility in single hyperbolic configuration of twining configuration, and can realize bigger effective stretching in preposition right cylinder and hyperbolic line contraction coupling micro-fluidic structure from the comparison diagram of Fig. 4 and Fig. 5.
30 groups not the DNA of isomorphism type shrink average extensibility in the coupling micro-fluidic structure at single hyperbolic configuration and preposition right cylinder and hyperbolic line comparison diagram as shown in Figure 6.Can find out significantly that from Fig. 6 preposition right cylinder of the present invention and hyperbolic line shrink the stretch validity of various configuration DNA chains of coupling micro-fluidic structure.
Claims (6)
1. DNA separating micro-fluidic chip, it is characterized in that comprising the sample inlet pool (1), entrance channel (2), the little contraction structure of hyperbolic line (4), slit conduit (5), the amplification suddenly mouthful (6) that are communicated with successively, outlet flow (7), waste liquid pool (8), be positioned at the little contraction structure of hyperbolic line (4) entrance channel (2) before and be provided with cylindrical obstacle (3), cylindrical obstacle (3) places on the fluid channel medullary ray.
2. DNA separating micro-fluidic chip according to claim 1 is characterized in that the length l of described entrance channel (2)
1=1.5～10mm, the width w of entrance channel (2)
1=30～200 μ m, the length l of the little contraction structure of hyperbolic line (4)
c=10～180 μ m, the length l of slit conduit (5)
2=0.01～1.54mm, the width w of slit conduit (5)
2=0.01～3.8 μ m, the length l of outlet flow (7)
3=1.5～10mm, the width w of outlet flow (7)
3=30～200 μ m.
3. DNA separating micro-fluidic chip according to claim 1 and 2 is characterized in that the equation of the little contraction structure of described hyperbolic line (4) is: the intersection point with fluid channel medullary ray and hyperbolic line ingress is a true origin,
Wherein, coefficient c=w
2l
c/ (2-2w
2/ w
1).
4. DNA separating micro-fluidic chip according to claim 1 is characterized in that the radius of described preposition cylindrical obstacle (3) can be adjusted as required, change in radius scope 5 μ m～75 μ m.
5. DNA separating micro-fluidic chip according to claim 1, the position that it is characterized in that described preposition cylindrical obstacle (3) can be adjusted as required, and the variable in distance scope of the little contraction structure of its width between centers hyperbolic line (4) ingress is 10 μ m～50 μ m.
6. DNA separating micro-fluidic chip according to claim 1 is characterized in that described unexpected amplification mouthful (6) is structure of right angle tyoe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100562738A CN101177663A (en) | 2007-11-05 | 2007-11-05 | DNA separating micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100562738A CN101177663A (en) | 2007-11-05 | 2007-11-05 | DNA separating micro-fluidic chip |
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| Publication Number | Publication Date |
|---|---|
| CN101177663A true CN101177663A (en) | 2008-05-14 |
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|---|---|---|---|
| CNA2007100562738A Pending CN101177663A (en) | 2007-11-05 | 2007-11-05 | DNA separating micro-fluidic chip |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102565171A (en) * | 2012-01-05 | 2012-07-11 | 厦门大学 | Microfluidic chip for isoelectric focusing separation |
| WO2013016873A1 (en) * | 2011-08-04 | 2013-02-07 | Zhang Hongpeng | Ship domestic sewage detection device |
| CN103212457A (en) * | 2013-05-20 | 2013-07-24 | 吉林大学 | Micro-fluidic chip for inhibiting electroosmotic flows through grafting polyelectrolyte brush on surface of micro-channel |
| CN104741157A (en) * | 2010-09-14 | 2015-07-01 | 加利福尼亚大学董事会 | Device for isolating cells from heterogeneous solution using microfluidic trapping vortices |
| CN110865113A (en) * | 2019-11-19 | 2020-03-06 | 复旦大学 | Method for modifying field effect transistor sensor interface based on DNA nano machine |
| CN113617402A (en) * | 2021-07-07 | 2021-11-09 | 福建农林大学 | Microfluidic chip and device for generating shearing and stretching combined flow field and application method thereof |
-
2007
- 2007-11-05 CN CNA2007100562738A patent/CN101177663A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104741157A (en) * | 2010-09-14 | 2015-07-01 | 加利福尼亚大学董事会 | Device for isolating cells from heterogeneous solution using microfluidic trapping vortices |
| WO2013016873A1 (en) * | 2011-08-04 | 2013-02-07 | Zhang Hongpeng | Ship domestic sewage detection device |
| CN102565171A (en) * | 2012-01-05 | 2012-07-11 | 厦门大学 | Microfluidic chip for isoelectric focusing separation |
| CN103212457A (en) * | 2013-05-20 | 2013-07-24 | 吉林大学 | Micro-fluidic chip for inhibiting electroosmotic flows through grafting polyelectrolyte brush on surface of micro-channel |
| CN103212457B (en) * | 2013-05-20 | 2015-01-07 | 吉林大学 | Micro-fluidic chip for inhibiting electroosmotic flows through grafting polyelectrolyte brush on surface of micro-channel |
| CN110865113A (en) * | 2019-11-19 | 2020-03-06 | 复旦大学 | Method for modifying field effect transistor sensor interface based on DNA nano machine |
| CN110865113B (en) * | 2019-11-19 | 2024-03-26 | 复旦大学 | Method for modifying field effect transistor sensor interface based on DNA nano machine |
| CN113617402A (en) * | 2021-07-07 | 2021-11-09 | 福建农林大学 | Microfluidic chip and device for generating shearing and stretching combined flow field and application method thereof |
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Open date: 20080514 |