CN101172995A - Method for producing two functional soybean separation proteins with membrane separation - Google Patents
Method for producing two functional soybean separation proteins with membrane separation Download PDFInfo
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- CN101172995A CN101172995A CNA2007101633919A CN200710163391A CN101172995A CN 101172995 A CN101172995 A CN 101172995A CN A2007101633919 A CNA2007101633919 A CN A2007101633919A CN 200710163391 A CN200710163391 A CN 200710163391A CN 101172995 A CN101172995 A CN 101172995A
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Abstract
The invention discloses a method of using an ultrafiltration membrane to prepare soybean separation albumen with two functions, and the method crushes bean pulp in low temperature, and adds deionized water for lixiviating, and then albumen extracting solution is obtained after eliminating bean dregs by centrifugal separation. The ultrafiltration membrane with cutting molecular weight 200000 of Da to 300000 Da is used as a first-level ultrafiltration membrane and a concentrated solution is sterilized and dried by spray, and then the fist-level membrane separation albumen with high gel property is obtained. First-level ultrafiltration penetrating liquid directly enters a second-level ultrafiltration system with the cutting weight of 8000 Da to 20000 Da, and the second-level membrane separation albumen is obtained by a second-level ultrafiltration concentrated solution through sterilization, enzyme killing, fishy smell eliminating and spray drying. The invention can produce the soybean separation albumen with two functions. Moreover, the invention has continuous technique, and saves energy consumption with small acid and alkali using quantity.
Description
Technical field
The invention belongs to the soybean deep processing technology field, utilize membrane separation technique to realize the preparation of two kinds of functional soy protein isolates.
Background technology
It is a high-new isolation technique that develops rapidly nearly decades that ultra-filtration membrane separates, and it has realized the separating substances of molecular level, is widely used in industries such as chemical industry, environmental protection, metallurgy, food.
In the soybean deep processing technology field application is arranged also.Picture patent publication No. CN1615722A, the ultra-filtration membrane isolation technique prepares the method for soybean protein isolate, uses ultra-filtration technique to separate the soybean protein isolate that has prepared high resolution with low temperature soy meal.It can only prepare a kind of soybean protein isolate of high resolution.High molecular weight protein and small molecular protein are not carried out fractional separation, the functional product that draws is single.
Summary of the invention
The technical problem to be solved in the present invention is: because the non-constant width of soy-protein molecular weight distribution, from 8,000 Da-60, ten thousand Da, the difference of protein molecular weight size, the protein function that draws is different, the protein gel of macromolecule is good, and the small molecular weight protein solvability is good.Utilize ultra-filtration membrane molecular weight cut off difference, fractional separation goes out high gelation soybean protein isolate and high resolution soybean protein isolate respectively.
For reaching above purpose, technical scheme of the present invention is:
(1) defatted soybean meal is pulverized, and adds 16 times of deionized water dissolvings, transfers PH7.6 with sodium hydroxide (NaoH), temperature 45-50 ℃, stirring and leaching 40-60 minute, gets the soybean protein vat liquor.
(2) the soybean protein vat liquor is removed insoluble bean dregs by centrifugation, gets the soybean protein extracting solution.
(3) one-level uf processing: step (2) is got the soybean protein extracting solution pump into the one-level ultrafiltration system, one-level ultra-filtration membrane molecular weight cut off 200,000 Da-30 ten thousand Da, realize high molecular weight protein and small molecular protein, oligose, the separation of salt, get high molecular weight protein one-level ultrafiltration and concentration liquid, its solid concentration 12%-15%, and obtain the one-level ultrafiltration membrane permeate liquid.
(4) after one-level ultrafiltration and concentration liquid is transferred PH7 with hydrochloric acid (HCl), be extracted into disinfection system and be extracted into spray drying system by pressure pump after second, get one-level membrane sepn albumen after the drying at sterilization 8-13 under the 130-150 ℃ of condition.
(5) two-stage ultrafiltering is handled: step (3) obtains the one-level ultrafiltration membrane permeate liquid and directly pumps into the two-stage ultrafiltering system, two-stage ultrafiltering film molecular weight cut off 8,000 Da-2 ten thousand Da, add 45-50 ℃ of deionized water to original volume after concentrating 10-15 times, need not transfer pH value, ultrafiltration and concentration is realized small molecular protein and oligose to solid content 10%-12% again, the separation of salt, get the secondary protein concentrated solution, and obtain the two-stage ultrafiltering film, enter post-order process and extract soybean oligosaccharide through liquid.
(6) the secondary protein concentrated solution is extracted into sterilization, enzyme goes out, take off the raw meat system, keep 4-8 second in 125-145 ℃ of temperature, feed liquid sprays in the vacuum tank then, and water vapour is taken beany flavor away, and after feed temperature reduced to 50-65 ℃ fast, by clarifixator, get to high-pressure pump behind the homogeneous and enter spray drying system, get secondary membrane sepn albumen after the drying.
Advantage of the present invention is:
1. one-level ultra-filtration membrane molecular weight cut off 200,000 Da-30 ten thousand Da, one-level ultrafiltration and concentration liquid mainly is the macromole sphaeroprotein like this, so one-level ultra-filtration membrane protein isolate gelation height, secondary membrane sepn albumen are that its solvability is fine like this based on floating small molecular protein clearly.Two kinds of functional products have been drawn synchronously.
2. the present invention uses the secondary continuous ultrafiltration, and separating technology is obtaining a kind of functional product step by step, also played simultaneously the pre-treatment effect of back one-level membrane process, make technology non-stop run in confined conditions, middle without temperature adjustment degree, pH value, the soda acid consumption is few, heating power consumption is few, saves running cost, is easy to realize automatization control, reduce labor intensity the cleaning procedure environment.
3. the continuous two-stage ultrafiltering of the present invention, the protein more than 98% all is trapped, the production process that second stage ultrafiltration membrane permeate liquid can directly enter soybean oligosaccharide in the operation (5).
The protein product performance index:
| Level membrane sepn albumen | Secondary membrane sepn albumen | |
| Protein: NSI: gel-strength (g/cm 2): robust fibre: ash content: | ≥90％ ≥85％ 4.56 0.82％ 2.31％ | ≥88％ ≥99％ 1.27 0.001％ 4.15％ |
Embodiment
Below embodiments of the invention are done and described in further detail.
Embodiment 1:
(1) gets defatted soybean meal 10kg, pulverize, add the 160kg deionized water dissolving, transfer PH7.6, temperature 45-50 ℃, stirring and leaching 40-60 minute, get the soybean protein vat liquor with sodium hydroxide (NaoH).
(2) vat liquor is removed insoluble bean dregs by centrifugation, gets soybean protein extracting solution 164.92kg.
(3) one-level uf processing: step (2) is got the soybean protein extracting solution pump into the one-level ultrafiltration system, one-level ultra-filtration membrane molecular weight cut off 300,000 Da, realize high molecular weight protein and small molecular protein, oligose, the separation of salt, get high molecular weight protein one-level ultrafiltration and concentration liquid, its solid concentration 12.27%, and obtain the one-level ultrafiltration membrane permeate liquid.
(4) after one-level ultrafiltration and concentration liquid is transferred PH7 with hydrochloric acid (HCl), be extracted into disinfection system, be extracted into spray drying system by pressure pump after second, get one-level membrane sepn albumen 2.9kg (protein butt 91.12%) after the drying at sterilization 8-13 under the 130-150 ℃ of condition.
(5) two-stage ultrafiltering is handled: step (3) obtains the one-level ultrafiltration membrane permeate liquid and directly pumps into the two-stage ultrafiltering system, two-stage ultrafiltering film molecular weight cut off 20,000 Da, add 45-50 ℃ of deionized water to original volume after concentrating 12 times, need not transfer pH value, ultrafiltration and concentration is realized small molecular protein and oligose, the separation of salt to solid content 10%-12% again, get the secondary protein concentrated solution, and obtain the two-stage ultrafiltering film through liquid.
(6) the secondary protein concentrated solution is extracted into sterilization, enzyme goes out, take off the raw meat system, in 125-145 ℃ of temperature, keep 4-8 second, feed liquid sprays in the vacuum tank then, and water vapour is taken beany flavor away, and after feed temperature reduced to 50-65 ℃ fast, enter spraying drying matter system by getting to high-pressure pump behind the clarifixator homogeneous, get secondary membrane sepn albumen 1.13kg (protein butt 88.5%) after the drying.
Embodiment 2:
(1) gets defatted soybean meal 10kg, pulverize, add the 160kg deionized water dissolving, transfer PH7.6, temperature 45-50 ℃, stirring and leaching 40-60 minute, get the soybean protein vat liquor with sodium hydroxide (NaoH).
(2) vat liquor is removed insoluble bean dregs by centrifugation, gets soybean protein extracting solution 164.92kg.
(3) one-level uf processing: step (2) is got the soybean protein extracting solution pump into the one-level ultrafiltration system, one-level ultra-filtration membrane molecular weight cut off 200,000 Da realize high molecular weight protein and small molecular protein, oligose, and the separation of salt gets high molecular weight protein one-level ultrafiltration and concentration liquid.Its solid concentration 13.1%, and obtain the one-level ultrafiltration membrane permeate liquid.
(4) after one-level ultrafiltration and concentration liquid is transferred PH7 with hydrochloric acid (HCl), be extracted into disinfection system, be extracted into spray drying system by pressure pump after second, get one-level membrane sepn albumen 3.1kg (protein butt 90.8%) after the drying at sterilization 8-13 under the 130-150 ℃ of condition.
(5) two-stage ultrafiltering is handled: step (3) obtains the one-level ultrafiltration membrane permeate liquid and directly pumps into the two-stage ultrafiltering system, two-stage ultrafiltering film molecular weight cut off 8,000 Da, add 45-50 ℃ of deionized water to original volume after concentrating 13 times, need not transfer pH value, ultrafiltration and concentration is realized small molecular protein and oligose, the separation of salt to solid content 10%-12% again, get the secondary protein concentrated solution, and obtain the two-stage ultrafiltering film through liquid.
(6) the secondary protein concentrated solution is extracted into sterilization, enzyme goes out, take off the raw meat system, in 125-145 ℃ of temperature, keep 4-8 second, feed liquid sprays in the vacuum tank then, and water vapour is taken beany flavor away, and after feed temperature reduced to 50-65 ℃ fast, enter spraying drying matter system by getting to high-pressure pump behind the clarifixator homogeneous, get secondary membrane sepn albumen 1.08kg (protein butt 88.2%) after the drying.
Claims (1)
1. the method for a producing two functional soybean separation proteins with membrane separation is characterized in that, is undertaken by following step:
(1) defatted soybean meal is pulverized, and adds 16 times of deionized water dissolvings, transfers PH7.6 with sodium hydroxide (NaoH), temperature 45-50 ℃, stirring and leaching 40-60 minute, gets the soybean protein vat liquor;
(2) the soybean protein vat liquor is removed insoluble bean dregs by centrifugation, gets the soybean protein extracting solution;
(3) one-level uf processing: step (2) is got the soybean protein extracting solution pump into the one-level ultrafiltration system, one-level ultra-filtration membrane molecular weight cut off 200,000 Da-30 ten thousand Da, realize high molecular weight protein and small molecular protein, oligose, the separation of salt, get high molecular weight protein one-level ultrafiltration and concentration liquid, its solid concentration 12%-15%, and obtain the one-level ultrafiltration membrane permeate liquid;
(4) after one-level ultrafiltration and concentration liquid is transferred PH7 with hydrochloric acid (HCl), be extracted into disinfection system and be extracted into spray drying system by pressure pump after second, get one-level membrane sepn albumen after the drying at sterilization 8-13 under the 130-150 ℃ of condition;
(5) two-stage ultrafiltering is handled: step (3) obtains the one-level ultrafiltration membrane permeate liquid and directly pumps into the two-stage ultrafiltering system, two-stage ultrafiltering film molecular weight cut off 8,000 Da-2 ten thousand Da, add 45-50 ℃ of deionized water to original volume after concentrating 10-15 times, need not transfer pH value, ultrafiltration and concentration is realized small molecular protein and oligose to solid content 10%-12% again, the separation of salt, get the secondary protein concentrated solution, and obtain the two-stage ultrafiltering film, enter post-order process and extract soybean oligosaccharide through liquid;
(6) the secondary protein concentrated solution is extracted into sterilization, enzyme goes out, take off the raw meat system, keep 4-8 second in 125-145 ℃ of temperature, feed liquid sprays in the vacuum tank then, and water vapour is taken beany flavor away, and after feed temperature reduced to 50-65 ℃ fast, by clarifixator, get to high-pressure pump behind the homogeneous and enter spray drying system, get secondary membrane sepn albumen after the drying.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101633919A CN101172995A (en) | 2007-10-24 | 2007-10-24 | Method for producing two functional soybean separation proteins with membrane separation |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210371A (en) * | 2010-04-06 | 2011-10-12 | 赤壁市赤壁红口口乐有限公司 | Process for extracting plant collagen |
| CN103053786A (en) * | 2012-12-17 | 2013-04-24 | 华南理工大学 | Preparation method for low-phytic acid calcium-tolerant soybean protein |
| CN103202384A (en) * | 2013-04-16 | 2013-07-17 | 华东师范大学 | Preparation method of acid soluble soy protein |
| CN105432934A (en) * | 2014-08-07 | 2016-03-30 | 中粮营养健康研究院有限公司 | Method for preparing soybean protein isolate |
| CN105949274A (en) * | 2016-07-08 | 2016-09-21 | 刘新旗 | Novel soybean peptide separating and refining method |
| CN108935915A (en) * | 2018-06-27 | 2018-12-07 | 山东禹王生态食业有限公司 | A kind of preparation method of smellless soybean separated protein powder |
| CN109734773A (en) * | 2019-03-06 | 2019-05-10 | 康哲(湖南)制药有限公司 | A kind of preparation method of protein hydrolysate concentrate |
| CN110574823A (en) * | 2019-09-27 | 2019-12-17 | 烟台双塔食品股份有限公司 | process method and device for extracting pea albumin from water |
-
2007
- 2007-10-24 CN CNA2007101633919A patent/CN101172995A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210371A (en) * | 2010-04-06 | 2011-10-12 | 赤壁市赤壁红口口乐有限公司 | Process for extracting plant collagen |
| CN103053786A (en) * | 2012-12-17 | 2013-04-24 | 华南理工大学 | Preparation method for low-phytic acid calcium-tolerant soybean protein |
| CN103202384A (en) * | 2013-04-16 | 2013-07-17 | 华东师范大学 | Preparation method of acid soluble soy protein |
| CN105432934A (en) * | 2014-08-07 | 2016-03-30 | 中粮营养健康研究院有限公司 | Method for preparing soybean protein isolate |
| CN105949274A (en) * | 2016-07-08 | 2016-09-21 | 刘新旗 | Novel soybean peptide separating and refining method |
| CN108935915A (en) * | 2018-06-27 | 2018-12-07 | 山东禹王生态食业有限公司 | A kind of preparation method of smellless soybean separated protein powder |
| CN109734773A (en) * | 2019-03-06 | 2019-05-10 | 康哲(湖南)制药有限公司 | A kind of preparation method of protein hydrolysate concentrate |
| CN110574823A (en) * | 2019-09-27 | 2019-12-17 | 烟台双塔食品股份有限公司 | process method and device for extracting pea albumin from water |
| CN110574823B (en) * | 2019-09-27 | 2022-04-22 | 烟台双塔食品股份有限公司 | Process method and device for extracting pea albumin from water |
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