CN101167747A - Bull semen diluent and its configuration method and adding method - Google Patents
Bull semen diluent and its configuration method and adding method Download PDFInfo
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- CN101167747A CN101167747A CNA2007100470914A CN200710047091A CN101167747A CN 101167747 A CN101167747 A CN 101167747A CN A2007100470914 A CNA2007100470914 A CN A2007100470914A CN 200710047091 A CN200710047091 A CN 200710047091A CN 101167747 A CN101167747 A CN 101167747A
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Abstract
The invention discloses a collocation process of cattle sperm dilution fluid, the cattle sperm dilution fluid gained from the collocation process and additive process of the cattle sperm dilution fluid. The collocation process is that a. weigh trishydroxymethylaminomethane, citric acid and fructose are weighed up accurately and charged with distilled water; b. 50,000-100,000 units of penicillin and streptomycin is charged into 100ml solution; c. the solution is stirred evenly until completely liquefying, and divide into liquid A and liquid B; d. incommensurate glycerol and distilled water are charged into the liquid A separately; e. vitelline are charged into the liquid A and the liquid B separately and stirred fully; f. the liquid A is covered and conserved into water bath vessel with 32 DEG C, the liquid B is covered and conserved into low temperature tank with 4 DEG C. When using, the liquid A is added in advance to dilute half of the gross and after the temperature is dropped to temperature equilibrium, the liquid B is charged for three times. The preparing process and the diluting process of the cattle sperm dilution fluid can better improve sperm activity and reduce the deformation rate of sperm.
Description
Technical field
The present invention relates to a kind of animal semen diluent and collocation method and adding method, relate in particular to a kind of bull semen diluent and collocation method thereof and adding method.
Background technology
The configuration selection chemical reagent of bull semen diluent in the prior art: trisodium citrate, fructose, lactose, sucrose, glycerol, egg, distilled water, penicillin, streptomycin.
Prescription one:
Yolk diluent: A liquid: 2.9% sodium citrate liquid 100.0ml, yolk 10.0%.
B liquid: get A liquid 41.75ml and add fructose 2.5 grams, glycerol 7.0ml.
Prescription two:
Milk diluent: skim milk 83.0ml, yolk 10.0ml, glycerol 7.0ml.
Add each 100,000 unit of penicillin and streptomycin among the every 100.0ml of all diluents, now with the current.
Wherein yolk is the main nutrition part of ovum, and yolk is the nutrient substance of oviparous animal in its egg, mainly is moisture, protein, nucleic acid.Alkaloid, mineral ions etc. are also arranged. coated outside vitellinae membrana, and there is a discous small particles in yolk surface central authorities, are called blastodisc, and nucleus is contained in the inside.Effect: for fetal development provides nutrition, reduce the consumption of sperm self-energy, so that prolong sperm life.
Diluent: mainly enlarge the seminal fluid capacity.Therefore the dosage of this class material must have identical osmotic pressure with seminal fluid, generally adopt isoosmotic sodium chloride, glucose, really, sucrose and some saline solution.
Protective agent: for the electrolyte concentration that reduces in the seminal fluid plays cushioning effect, generally being used for buffered material has sodium citrate, sodium hydrogen phosphate, potassium dihydrogen phosphate etc.Glycerol can prevent the effect of sperm cold shock
Antibiotic: to stop bacterial growth, common antibiotics has penicillin, streptomycin.
The semen dilution adding method:
Adopt double dilution method: with adding diluent be divided into secondary and add, at first add not glycerinated A liquid, add to half of dilution total amount, fill up the B liquid that contains glycerol again after cooling, the balance.
Summary of the invention
Technical problem to be solved by this invention is that a kind of motility of sperm that more helps improving will be provided, and reduces the collocation method of the bull semen diluent of rate of teratosperm; And a kind of bull semen diluent that obtains with the said method configuration; And the adding method of above-mentioned bull semen diluent.
For the collocation method of bull semen diluent of the present invention, above-mentioned technical problem is solved like this:
A. accurately take by weighing Tris 12.1 gram, citric acid 6.7 grams, fructose 5.0 and restrain in the 500ml beaker, adding distil water is to 250ml;
B. the penicillin and streptomycin that adds 5-10 ten thousand units in every 100ml solution; (penicillin and streptomycin are human)
C. wait to stir, after medicine dissolves fully, on average pour in two beakers and (be divided into A liquid and B liquid);
D. in A liquid, add 6ml glycerol, and add distilled water to 200ml; In B liquid, add 26ml glycerol, and adding distil water is to 200ml;
E. in A liquid, B liquid, add 50ml yolk and fully stirring respectively;
Put into 32 ℃ of water-baths after A liquid added a cover and preserve, put into 4 ℃ of low temperature cabinets after B liquid is added a cover and preserve.
For bull semen diluent of the present invention, obtain by above-mentioned collocation method configuration, include fructose, glycerol, egg, distilled water, penicillin, streptomycin, but also include Tris and citric acid.
For the adding method of bull semen diluent of the present invention, above-mentioned technical problem is solved like this:
At first add A liquid, add to half of dilution total amount; Add B liquid again after the cooling balance, B liquid divides three interpolations, adds 1/6 of dilution total amount at every turn.
Adopt Tris (Tris) as buffer reagent in the cattle seminal fluid thin liquid of the present invention, semen dilution there is certain buffer protection function, help improving the motility of sperm after the dilution, because Tris is a kind of ealkaline buffer, sperm metabolism poisoning and the movable reaction of enzyme had good cushioning effect; Replace sodium citrate with citric acid, can reduce the content of sodium ion in the diluent, help improving motility of sperm, reduce rate of teratosperm; Add suitable glycerol in the A liquid, in the cooling equilibrium process, play certain low-temperature protection effect, improve sperm and freeze the back vigor.
Except that the glycerol content difference, all the other compositions are all identical in A, B two liquid, so just can make the formulation operations of diluent easier.
And the adding method of diluent provided by the invention, B liquid divides three interpolations in dilution interpolation process, can reduce like this because of the osmotic pressure flip-flop to cause sperm death.
Bull semen diluent and dilution adding method thereof according to method configuration of the present invention more helps improving motility of sperm, reduces rate of teratosperm in a word.
The specific embodiment
The present invention is described further below in conjunction with specific embodiment.
Used chemical reagent: Tris, citric acid, fructose, glycerol, egg, distilled water, penicillin, streptomycin
Compound method:
1. accurately take by weighing Tris12.1 gram, citric acid 6.7 grams, fructose 5.0 and restrain in the 500ml beaker, adding distil water is to 250ml.
2. the penicillin and streptomycin that adds 5-10 ten thousand units in every 100ml solution.(penicillin and streptomycin are human)
3. wait to stir, after medicine dissolves fully, on average pour in two beakers and (be divided into A liquid and B liquid).
4. in A liquid, add 6ml glycerol, and add distilled water to 200ml; In B liquid, add 26ml glycerol, and adding distil water is to 200ml.
5. in A liquid, B liquid, add 50ml yolk and fully stirring respectively.
6. put into 32 ℃ of water-baths after A liquid being added a cover and preserve, put into 4 ℃ of low temperature cabinets after B liquid is added a cover and preserve
Adding method:
Double dilution method: with adding diluent be divided into secondary and add, at first add A liquid, add to half of dilution total amount; Add B liquid again after the cooling balance, B liquid divides three interpolations, adds 1/6 of dilution total amount at every turn.
Claims (3)
1. the collocation method of a bull semen diluent,
A. accurately take by weighing Tris 12.1 gram, citric acid 6.7 grams, fructose 5.0 and restrain in the 500ml beaker, adding distil water is to 250ml;
B. the penicillin and streptomycin that adds 5-10 ten thousand units in every 100ml solution;
C. wait to stir, after medicine dissolves fully, on average pour in two beakers and (be divided into A liquid and B liquid);
D. in A liquid, add 6ml glycerol, and add distilled water to 200ml; In B liquid, add 26ml glycerol, and adding distil water is to 200ml;
E. in A liquid, B liquid, add 50ml yolk and fully stirring respectively;
F. put into 32 ℃ of water-baths after A liquid being added a cover and preserve, put into 4 ℃ of low temperature cabinets after B liquid is added a cover and preserve.
2. the bull semen diluent that makes in accordance with the method for claim 1 includes fructose, glycerol, egg, distilled water, penicillin, streptomycin, it is characterized in that: described bull semen diluent also includes Tris and citric acid.
3. the adding method of a bull semen diluent that makes in accordance with the method for claim 1 at first adds A liquid, adds to half of dilution total amount; Add B liquid again after the cooling balance, B liquid divides three interpolations, adds 1/6 of dilution total amount at every turn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA2007100470914A CN101167747A (en) | 2007-10-16 | 2007-10-16 | Bull semen diluent and its configuration method and adding method |
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| CNA2007100470914A CN101167747A (en) | 2007-10-16 | 2007-10-16 | Bull semen diluent and its configuration method and adding method |
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| CN101167747A true CN101167747A (en) | 2008-04-30 |
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| CNA2007100470914A Pending CN101167747A (en) | 2007-10-16 | 2007-10-16 | Bull semen diluent and its configuration method and adding method |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999342A (en) * | 2010-10-08 | 2011-04-06 | 内蒙古蒙牛繁育生物技术股份有限公司 | Novel preparation method of yolk diluent |
| CN102318596A (en) * | 2011-07-12 | 2012-01-18 | 青岛康大食品有限公司 | Diluent for preserving rabbit sperms at normal temperature and preparation method thereof |
| CN102948415A (en) * | 2012-11-20 | 2013-03-06 | 新疆天山畜牧生物工程股份有限公司 | Diluent for frozen cattle semens and preparation method of diluent |
| CN103004750A (en) * | 2012-11-27 | 2013-04-03 | 云南省畜牧兽医科学院 | Livestock semen freezing diluent as well as preparation method and application thereof |
| CN103704203A (en) * | 2014-01-03 | 2014-04-09 | 甘肃省畜牧兽医研究所 | Preparation method and using method for GL and TCM199 serving as bovine semen diluent and bovine semen refrigerating fluid |
| CN103766325A (en) * | 2014-01-02 | 2014-05-07 | 甘肃省畜牧兽医研究所 | Preparation and use methods for EG (ethylene glycol) and TCM199 serving as bovine semen diluent and bovine semen freezing liquid |
| CN104012520A (en) * | 2014-05-23 | 2014-09-03 | 山西省生态畜牧产业管理站 | Anti-oxidative cattle frozen semen diluent and preparation method thereof |
| CN106538511A (en) * | 2016-09-30 | 2017-03-29 | 马伟 | A kind of manufacture method of antioxidative sheep freezing seminal fluid dilution |
| CN106818709A (en) * | 2017-02-06 | 2017-06-13 | 潘越博 | Cattle freezing seminal fluid dilution preparation method |
| CN107212945A (en) * | 2017-06-20 | 2017-09-29 | 金寨县胜华娃娃鱼开发有限公司 | Preparation and application for the high-efficiency agent for dilution liquid of giant salamander seminal fluid |
| CN107396918A (en) * | 2016-11-15 | 2017-11-28 | 青海省家畜改良中心 | It is a kind of to be used to preserve dilution for hiding sheep seminal fluid and preparation method thereof for a long time |
| CN110463688A (en) * | 2019-07-10 | 2019-11-19 | 安徽农业大学 | A kind of dilution and store method of sheep sperm |
| CN112640886A (en) * | 2020-12-18 | 2021-04-13 | 洛阳市洛瑞牧业有限公司 | Cattle frozen semen diluent and preparation method thereof |
| CN114208813A (en) * | 2021-12-23 | 2022-03-22 | 北京奶牛中心 | Cow frozen semen diluent, preparation method thereof and semen freezing method |
-
2007
- 2007-10-16 CN CNA2007100470914A patent/CN101167747A/en active Pending
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999342B (en) * | 2010-10-08 | 2012-11-07 | 内蒙古赛科星繁育生物技术股份有限公司 | Novel preparation method of yolk diluent |
| CN101999342A (en) * | 2010-10-08 | 2011-04-06 | 内蒙古蒙牛繁育生物技术股份有限公司 | Novel preparation method of yolk diluent |
| CN102318596A (en) * | 2011-07-12 | 2012-01-18 | 青岛康大食品有限公司 | Diluent for preserving rabbit sperms at normal temperature and preparation method thereof |
| CN102948415A (en) * | 2012-11-20 | 2013-03-06 | 新疆天山畜牧生物工程股份有限公司 | Diluent for frozen cattle semens and preparation method of diluent |
| CN103004750A (en) * | 2012-11-27 | 2013-04-03 | 云南省畜牧兽医科学院 | Livestock semen freezing diluent as well as preparation method and application thereof |
| CN103004750B (en) * | 2012-11-27 | 2014-07-02 | 云南省畜牧兽医科学院 | Livestock semen freezing diluent as well as preparation method and application thereof |
| CN103766325B (en) * | 2014-01-02 | 2017-01-18 | 甘肃省畜牧兽医研究所 | Preparation and use methods for EG (ethylene glycol) and TCM199 serving as bovine semen diluent and bovine semen freezing liquid |
| CN103766325A (en) * | 2014-01-02 | 2014-05-07 | 甘肃省畜牧兽医研究所 | Preparation and use methods for EG (ethylene glycol) and TCM199 serving as bovine semen diluent and bovine semen freezing liquid |
| CN103704203A (en) * | 2014-01-03 | 2014-04-09 | 甘肃省畜牧兽医研究所 | Preparation method and using method for GL and TCM199 serving as bovine semen diluent and bovine semen refrigerating fluid |
| CN103704203B (en) * | 2014-01-03 | 2017-01-18 | 甘肃省畜牧兽医研究所 | Preparation method and using method for GL and TCM199 serving as bovine semen diluent and bovine semen refrigerating fluid |
| CN104012520A (en) * | 2014-05-23 | 2014-09-03 | 山西省生态畜牧产业管理站 | Anti-oxidative cattle frozen semen diluent and preparation method thereof |
| CN106538511A (en) * | 2016-09-30 | 2017-03-29 | 马伟 | A kind of manufacture method of antioxidative sheep freezing seminal fluid dilution |
| CN107396918A (en) * | 2016-11-15 | 2017-11-28 | 青海省家畜改良中心 | It is a kind of to be used to preserve dilution for hiding sheep seminal fluid and preparation method thereof for a long time |
| CN106818709A (en) * | 2017-02-06 | 2017-06-13 | 潘越博 | Cattle freezing seminal fluid dilution preparation method |
| CN107212945A (en) * | 2017-06-20 | 2017-09-29 | 金寨县胜华娃娃鱼开发有限公司 | Preparation and application for the high-efficiency agent for dilution liquid of giant salamander seminal fluid |
| CN110463688A (en) * | 2019-07-10 | 2019-11-19 | 安徽农业大学 | A kind of dilution and store method of sheep sperm |
| CN110463688B (en) * | 2019-07-10 | 2021-07-27 | 安徽农业大学 | Diluent of sheep semen and preservation method |
| CN112640886A (en) * | 2020-12-18 | 2021-04-13 | 洛阳市洛瑞牧业有限公司 | Cattle frozen semen diluent and preparation method thereof |
| CN114208813A (en) * | 2021-12-23 | 2022-03-22 | 北京奶牛中心 | Cow frozen semen diluent, preparation method thereof and semen freezing method |
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Application publication date: 20080430 |