CN101156879A - Application of hippophae rhamnoides fruit oil in the preparation of promoting mankind spermatozoon generation and both sexes aphoria medicine - Google Patents
Application of hippophae rhamnoides fruit oil in the preparation of promoting mankind spermatozoon generation and both sexes aphoria medicine Download PDFInfo
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Abstract
The invention relates to an application of seabuckthorn fruit oil and/or whole fruit oil in preparing medicine promoting the formation of male sperms, preventing the apoptosis of spermatogenic cells or promoting the formation of female ovarian follicles and the maturation of germ cells and treating male and female sterility. By taking the preparation, kidney deficiency and infirmity and various chronic diseases can be recovered to the health status, more effectively, the invention has the functions of promoting the formation of the male sperms and protecting the quantity of the spermatogenic cells, and preventing the apoptosis of spermatogenic cells, and can obviously increase the quantity and the motor ability of the sperms, and increase the weight of spermary, and simultaneously has the functions of promoting the formation of the female ovarian follicles and the maturation of the germ cells, thereby being used as the medicine for the clinical treatment of male and female sterility. Compared with other medicines, the medicine has simple ingredients, belongs to target organ excitant, and has the reversible automatic regulation function of a second messenger, thereby being the medicine with high curative effect, security, and simplification.
Description
Technical field
The invention belongs to the medicinal application of plant extract, be used to recuperate under medical treatment men and women's sexual reproduction dysfunction.Both but promoting mankind spermatozoon generates, prevents male's spermatogenesis daughter cell atrophy apoptosis, also can urge women's follicle and generate, urgees maturation of ovum.Can be used for treating men and women's sterility.
Background technology
Progress and prosperity along with society, the ratio of male infertility is also progressively rising among the crowd. through in December, 2003 China andrology magazine report, the male infertility ratio may will be higher than more than the 12-16% in the future. and the male infertility ratio rises, decay has appearred in the reproductive performance that the male has been described, the competition (mental pressure) of this and social environment, (sleep is few for environmental pollution and self custom, smoke, insobriety, motion is few) direct association all arranged. the stimulation of extraneous factor usually can cause the imbalance of male endocrine, be one of inducement of male infertility. the main clinical condition of illness of infertility is atrophy of male's spermatogenic cell or apoptosis, even the few nothing of sperm quantity, motility of sperm is poor, and spermary is in light weight etc. and Therapeutic Method clinically is many according to the spermatogenic cell function, indexs such as the cause of disease relation of sub-ability of spermatogenesis (level) and infertility and patient's levels of reproductive hormones and semen quality are selected.Adopt the isolated natural 10 peptide ammino acid preparation for treating male sterilities of pig hypothalamus as external Schwarz stein.Or adopt human gonadotropin's medicine etc., some effects can be arranged.Sexual disorder is then main lowly relevant with hypogonadism or gonad function.Clinical method commonly used is hormone therapy, to promote manlike growth and acquired function.But it is inoperative to reproductive function or spermatogenesis.Also has estrogen antagonist medicine treatment etc.In a word, the male can not fundamentally improve its reproductive function because apoptosis of spermatogenic cells (few sperm), the infertility that the external environment factor causes the endocrine disturbance to cause all can not really be improved the spermatogenesis subfunction with androgen or anti-estrogens medicine merely.Particularly said medicine all has side effect in various degree, depression, visual deterioration, nauseating, dizzy, allergic dermatitis and alopecia etc. can occur.Also there are some Chinese medicine preparation to be used for auxiliary therapy infertility (short spermatogenesis) clinically.Commonly used have a kidney-brain-nourishing tablet (the version pharmacopeia was recorded in 2005).But really from morphology, can confirm the rare so far report of medicine that the promotion spermatid generates.Identical with the male genetic obstacle is that female reproductive function obstacle such as infertility, follicular dysplasia etc. also exist similar problem.
Summary of the invention
The objective of the invention is to overcome the deficiency of existing Chinese and western drugs Therapeutic Method, but provide a kind of both promoting mankind spermatozoons to generate, prevent male's spermatogenesis daughter cell atrophy apoptosis, the medicine that women's follicle generates, urgees maturation of ovum be can urge again, treatment man, female infertility can be used for.
For this reason, the present invention proposes Oleum Hippophae and/or the application of full fruit oil in the generation of preparation promoting mankind spermatozoon, prevention apoptosis of spermatogenic cells or the generation of short women's follicle, short maturation of ovum and both sexes aphoria medicine.Can treat no sperm, diseases such as few sperm (apoptosis of spermatogenic cells .) or follicular dysplasia.
Test shows that the real oily single medicinal material of sea-buckthorn whole fruit, Oleum Hippophae single medicinal material and the two can both obtain better effects by the blended preparation of arbitrary proportion.Wherein with the real oily blending constituent best results of sea-buckthorn whole fruit.The real oil of the said sea-buckthorn whole fruit of the present invention is meant the mixed oil (main component be fatty acid mixt) of full fruit (comprising sarcocarp, seed, peel) with organic solvent extraction.General with organic solvent chloroform, own gastral cavity etc. just, under temperature 30-70 ℃, pressure 0.3MPa-1.0MPa, from the full sea buckthorn fruit of drying and crushing, extract and make.Oleum Hippophae is meant the fruit juice oil that extracts or exempts from the fruit oil that heating is extracted through the physics centrifugalize from puree (the fresh fruit bits after fresh fruit presses fruit for cider) from the seabuckthorn plant fresh fruit.The fruit juice oil that the physics method is extracted is close with the puree oil component, has similar therapeutic effect in the present invention.The two can mix use by arbitrary proportion, all belongs to the category of fruit oil.
For ease of oral and carry, can be prepared into multiple dosage forms such as soft capsule, oral liquid and solid hard capsule.
Independent Oleum Hippophae, full fruit oil and the various mixing formulas of the two confirm through the animal pharmacodynamics: have the manifest function of regulating reproductive endocrine, can pass through to regulate polyceptor and the indirect regulation reproductive endocrine function, have short spermatogenesis (increasing spermatogenic cell quantity, short spermatogenesis and vigor strengthens) and stop the effect of spermatogenic cell atrophy or apoptosis, and then can treat many male infertilitys.Also can improve male's sexual function.
The specific embodiment
27 of the aged male rats of SD, (30 monthly age), blue or green 8 of rats in age (4 monthly age) are adopted in this experiment.Random packet is aged blank group, methyl testis group, calcium gluconate group, Fructus Hippophae line of oils, blank blue or green age group.Molten hydrops body such as be mixed with, under same environment, gavage suitable medicine 1.5-8 gram/kilogram to animal subject respectively./ 70 kilograms of human trial 1.5-8 grams.Detailed content is omitted.
Below the real oil of sea-buckthorn whole fruit, rat, 3 gram/kilograms are adopted in test.
Drug effect morphology result
One, testis and epididymis are organized drug effect morphology test
(illustrate: following A, B, D group all have aged male rat (30 monthly age) testis, epididymis organizational structure photo; The C group has blue or green male rat in age (4 monthly age) testis, epididymis organizational structure photo; E group has the take medicine aged male mouse testis of group, epididymis organizational structure photo of the real oil of sea-buckthorn whole fruit for reference, not attached, can offer at any time as required)
(1) A group-aged male rat blank group:
1, testis tissue is 6, and wherein 3 routine albuginea testises thicken, and the spermatogenic cell of convoluted seminiferous tubule reduces in various degree, and part spermatogenic epithelium cell is short, is monolayer alignment; The part convoluted seminiferous tubule diminishes, basement membrane thickened, between the part qualitative change wide, present atrophy and change.Sperm significantly reduces in the obvious degeneration of minority convoluted seminiferous tubule spermatogenic cell, tube chamber.Do not see connective tissue proliferation and cell infiltration in the interstitial tissue of testis.Other 3 testes histological structure are normal substantially.
2, the epididymis tissue is 7, and the epididymal duct epithelium is the low column of monolayer, and lumen of gland is bigger, and the part lumen of gland contains a large amount of sperms.Part epididymal duct epithelium is the cilium columnar epithelium.Light weight degree limitation hypertrophy between the minority epididymis, but do not see cell infiltration.Matter is not seen proliferation of fibrous tissue and cell infiltration between most epididymises.Outlet tube is seldom seen.(blank organizational structure picture A 1-A2, not attached)
(2) the male aged group of B group-clothes Methyl testosterone
1, testis tissue is 6, wherein has the interior spermatogenic cells at different levels of convoluted seminiferous tubule of 3 testis tissues to reduce in various degree, and cell is little, and is short, and the part convoluted seminiferous tubule diminishes.The obvious degeneration of minority convoluted seminiferous tubule spermatogenic cell, the cellular swelling, loose light the dying of endochylema is abortive haul shape structure.Sperm obviously reduces in the tube chamber; Tunica albuginea obviously thickens; Do not see connective tissue proliferation and cell infiltration in the interstitial tissue of testis.All the other 3 testes histological structure are normal substantially.(control tissue of taking medicine structure photo B1-B2, not attached).
2, the epididymis tissue is 6, and organizational structure is normal substantially.Epididymal duct is made of the low columnar epithelium of monolayer, and the chamber is bigger, and a large amount of sperms are arranged in the part tube chamber.Part epididymal duct epithelium is the cilium columnar epithelium, and outlet tube is seldom seen.Matter is not seen cell infiltration and connective tissue proliferation between epididymis.
(3) C group-male young blank group (4 monthly age)
1, testis tissue is 8, and organizational structure is normal substantially.Visible spermatogenic cells at different levels in the Qu Jingguan, its quantity, form and arrangement are normal substantially.Do not see connective tissue proliferation and cell infiltration in the interstitial tissue of testis.
2, the epididymis tissue is 7, and organizational structure is normal substantially.Epididymal duct is made of the low columnar epithelium of monolayer, and the chamber is bigger, and tube chamber contains a large amount of sperms.Part epididymal duct epithelium is the cilium columnar epithelium, and matter is not seen cell infiltration and connective tissue proliferation between epididymis.(blank Mus epididymis organizational structure photo C1-C2 in blue or green age, not attached)
(4) the male old rats group of D group-clothes calcium gluconate
1, testis tissue is 7, and wherein 4 routine testes histological structure are normal substantially, visible spermatogenic cells at different levels in the Qu Jingguan, and its quantity, form and arrangement are normal substantially, do not see connective tissue proliferation and cell infiltration in the interstitial tissue of testis.The spermatogenic cell of other 3 blocks of convoluted seminiferous tubules reduces in various degree, and wherein a routine spermatogenic cell almost disappears; The part necrocytosis, the Interstitial cell kitchen range shape hypertrophy of a routine testis, a matter small artery obviously thickens, and structural pipe wall is unclear, and connective tissue proliferation companion inflammatory cell is invaded profit around the blood vessel, and the tunica albuginea stage thickens.
2, the epididymis tissue is 7, and organizational structure is normal substantially.Light cell hypertrophy in the part epididymal duct is comparatively obvious, and the intracavity sperm is less.Part sperm tube epithelium is the cilium columnar epithelium, and a matter is not seen cell infiltration and connective tissue proliferation.(the male old rats testis of calcium gluconate, epididymis organizational structure picture B shows 1-D2-D3, not attached).
(5) E group--the real oily aged male rat of sea-buckthorn whole fruit is for the reagent group
1, testis tissue is 7, and structure is normal substantially.Visible spermatogenic cells at different levels in the Qu Jingguan, its quantity and form and arrangement are normal substantially, do not see connective tissue proliferation and cell infiltration in the interstitial tissue of testis.(the real oily aged male rat testes histological structure photo E1 of sea-buckthorn whole fruit, not attached)
2, the epididymis tissue is 7, and minority epididymis light cell slightly increases, and epithelial structure is normal substantially, and a large amount of sperms is arranged in the tube chamber.Part epididymal duct epithelium is the cilium columnar epithelium, and a matter is not seen fibrous connective tissue hypertrophy and cell infiltration. (same with green grass or young crops Mus in age, not attached photo)
Two, short spermatogenesis and prevention apoptosis result of the test
Above-mentioned result of study shows that the effect of the real oily medicine group of sea-buckthorn whole fruit is better than aged male rats blank group, Methyl testosterone matched group and calcium gluconate matched group greatly.The drug action of this group closely is same as the blue or green effect that age, Mus was organized.Experimentation confirms: 1, the real oil of sea-buckthorn whole fruit can stop the atrophy and the apoptosis of senile rat spermatogenic cell; and have function .2 that old male Mus is developed to young Mus function attitude direction, the real oil of sea-buckthorn whole fruit has old male mouse spermatogenic cell function of protection and short spermatogenesis, the function of short motility of sperm.3. above-mentioned result of study shows: 1) the downright bad degeneration of aged blank Mus testis and epididymis spermatogenic cell reaches 50%, and matter all has kitchen range shape inflammation between the kidney of the aged Mus of this group. and (seeing A3); 2) the spermatogenic cell obstacle appears in the matched group aged Mus of obeying 50% ratio that accounts for behind the Methyl testosterone merely, and promptly simple androgen can not be urged spermatogenesis.3) replenish the calcium aged Mus result shows: aged Mus is replenished the calcium merely, can cause the damage of spermatogenesis daughter cell and the spermatogenesis daughter cell is downright bad and the extinction of spermatogenesis daughter cell, and can cause the necrosis of kidney cell.4) the take medicine drug action of aged Mus of the real oil of sea-buckthorn whole fruit closely is same as blue or green age group, effect to testis and epididymis is all similar to green grass or young crops mouse in age, can repair the renal function of aged Mus, and organize identical (seeing E2) with the blue or green Ren Mus coagulation of YIN-cold in ZANG-organ in age structure. comparative study shows: the real oily drug action of sea-buckthorn whole fruit is not to act directly on the reproduction target organ to play a role, but medicine is brought into play the effect of the integrally-regulated function of courier's thing in vivo.
Three, the observation of human body curative effect (100 example): with the medicine of the real oil of sea-buckthorn whole fruit as the clinical treatment male infertility, few or the person of nothing for sperm quantity, can improve motility of sperm, improve spermary weight, change male's kidney deficiency and bodily weakness condition of illness, reach the effect of short spermatogenesis and short smart vigor, receive remarkable result.Method: take the real oil of 6 gram sea-buckthorn whole fruits every day, 46% obtain fertility among the infertility experimenter clinically.Other 58 obvious improvers of experimenter's self-induction function have 33 people, and it is about 20% that the sexual life number of times on average increases, and life quality generally improves.All the other people also have to a certain degree improvement.9 people's numbnesss only.Take continuously more than 3 years, do not find any side effect.Genus is simplified, safe drugs.
This research is write out a prescription design in addition together to the experiment at (22 monthly age) of female aged Mus with the real oil of sea-buckthorn whole fruit.(controlled trial group: green grass or young crops months old rats in ages 4, the aged Mus group of climacteric health medication, aged Mus blank group, the aged Mus group of estradiol, Oleum Hippophae medication old-age group Mus group) result is as follows:
Histological observation:
1, ovary
The follicle of visible most different developmental phases in the blue or green age Mus ovary, and see Table the corpus luteum that differs in size of face projection, the lutein cell structure is normal.
Blank aged matched group ovary basic structure with blue or green age the Mus group similar, but follicle quantity at different levels obviously reduces, as seen corpus luteum and lean type, cellularity is normal.
Estradiol group and aged Mus blank group compare, follicle number showed increased at different levels, and especially big growing follicle is seen more, but the apparent in view minimizing of the blue or green Mus in age of corpus luteum number.
The blank group of Fructus Hippophae line of oils and aged Mus compares, and follicle numbers at different levels increase, and big follicle increases, but it is many to be not so good as the estradiol group. and the interstitial gland that forms after the follicular atresia increases to some extent.Corpus luteum number and cellularity thereof are similar to green grass or young crops mouse matched group in age.
2, uterus
Green grass or young crops Mus in age group endometrial epithelium is the monolayer column, and intrinsic bed thickness contains uterine glands single or in heaps, and seeing in the lamina propria has many oxyphil cells; Two layers of the many branches of flesh layer, adventitia is thin.
Old-age group's blank group endometrium and Uterus wall thickness and the relatively attenuation of blue or green Mus in age, uterine glands is less in the lamina propria, also visible oxyphil cell, each constituent is similar to green grass or young crops Mus in age group.
The estradiol group compares with green grass or young crops Mus in age group, and the uterus increases slightly, Uterus wall, and endometrium and myometrium all obviously thicken, and cavity of uterus enlarges, and uterine glands increases, and is irregular as the chamber enlarges.
The oily in fact aged Mus group of sea-buckthorn whole fruit compares with green grass or young crops Mus in age after taking medicine, and can see Uterus wall and obviously thicken, and uterine glands increases, and can see the identical more oxyphil cell that has with green grass or young crops Mus in age in the lamina propria, and lumen of gland is rule.
Above result of the test all has photo in kind, can enclose at any time when needing.
More than studies confirm that: all kinds of Oleum Hippophaes really can make aged female Mus reproductive function to blue or green mouse direction conversion in age.The present invention has same identical effect to improving female reproductive function in addition, can obviously promote the generation of women's follicle and have the function of short maturation of ovum, therefore can reach the ability of improvement, treatment women reproductive function obstacle, raising women 's fertility function.
About Oleum Hippophae and with the mix preparation of full fruit oil test data to female male rat and men and women's property, all proved good result, repeat no more.
Except that the full fruit oil of Fructus Hippophae, Oleum Hippophae and the mix preparation of the two can both obtain similar effect.The leaf of seabuckthorn plant and other extract in addition, RUGEN, stem etc. also have certain curative effect through test.All belong to protection scope of the present invention.As space is limited, can not give unnecessary details one by one.Relevant further detailed pharmacological action and drug efficacy study data all can be enclosed at any time, and be for reference.
In a word, the present invention not only has the function of short spermatogenesis, and short follicle generates and the function of short maturation of ovum but also have.Have and make aged Mus reproductive function to the blue or green trend that age, direction transformed.Short really spermatogenesis, the maintenance ovary, short follicle generates, and treatment men and women's property is sterile, the active drug of infertility.
This patent is on the basis to the research of seabuckthorn plant effective ingredient, finds its another novel medical use.
Seabuckthorn plant belongs to Elaeangnaceae, machaka or dungarunga.The used Fructus Hippophae kind of the present invention is Chinese sea buckthorn (Hippophae rhamnoides L sinesp sinensis Rousi), generally is distributed in provinces and regions such as northwest, northeast, Gansu, Shanxi and Inner Mongol.
The extraction process of Oleum Hippophae adopts the physical extraction method.Its method is: place dull and stereotyped squeezer to squeeze sea-buchthorn fresh fruit, juice is by filling formula filter, the fine straining 4 times of pressurizeing continuously, and pressurization at last enters newborn ester formula centrifuge and separates, and can obtain Oleum Hippophae.
The real oil of sea-buckthorn whole fruit, puree oil, the preparation of leaf wet goods also all adopts conventional method to make, and the professional person all can implement, and does not give unnecessary details.
Up to now, still do not have employing Oleum Hippophae or full fruit oil treatment male's spermatogenic cell atrophy and sexual function degeneration both at home and abroad and change the Drugdoc report that the spermatogenic cell that causes reduces, testis is bred deterioration.Seemingly also there is not the comparatively ideal medicine that prevents that atrophy of elderly men spermatogenic cell or function degeneration from changing on the market.To the women also is same.The Fructus Hippophae soft capsule dosage form that utilizes the present invention to prepare, empirical tests really can be treated both sexes aphoria and protectiveness function degeneration diseases of aging.Genus is simplified, safe drugs.Be the valuable new invention of field of medicaments, for finding first both at home and abroad and disclosing.
Claims (3)
1. Oleum Hippophae and/or the sea-buckthorn whole fruit application of oil in the generation of preparation promoting mankind spermatozoon, prevention apoptosis of spermatogenic cells or the generation of short women's follicle, short maturation of ovum and both sexes aphoria medicine in fact.
2. Oleum Hippophae as claimed in claim 1 generates, stops apoptosis of spermatogenic cells or short women's follicle to generate, urge the application in maturation of ovum and the both sexes aphoria medicine at the preparation promoting mankind spermatozoon, it is characterized in that said Oleum Hippophae is to be become by fruit juice oil and/or puree line of oils.
3. the sea-buckthorn whole fruit as claimed in claim 1 application of oil in the generation of preparation promoting mankind spermatozoon, prevention apoptosis of spermatogenic cells or the generation of short women's follicle, short maturation of ovum and both sexes aphoria medicine in fact is characterized in that the real oil of said sea-buckthorn whole fruit comprises fruit juice oil, puree oil and seed oil.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114246887A (en) * | 2021-12-31 | 2022-03-29 | 宁夏医科大学总医院 | Application of plant seed oil in protecting testicle function |
| CN114288329A (en) * | 2021-12-31 | 2022-04-08 | 宁夏医科大学总医院 | Function and application of plant fruit pulp in protecting male fertility |
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2006
- 2006-12-30 CN CNA2006101053622A patent/CN101156879A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114246887A (en) * | 2021-12-31 | 2022-03-29 | 宁夏医科大学总医院 | Application of plant seed oil in protecting testicle function |
| CN114288329A (en) * | 2021-12-31 | 2022-04-08 | 宁夏医科大学总医院 | Function and application of plant fruit pulp in protecting male fertility |
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