CN101153032B - Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave - Google Patents
Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave Download PDFInfo
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- CN101153032B CN101153032B CN2006101525394A CN200610152539A CN101153032B CN 101153032 B CN101153032 B CN 101153032B CN 2006101525394 A CN2006101525394 A CN 2006101525394A CN 200610152539 A CN200610152539 A CN 200610152539A CN 101153032 B CN101153032 B CN 101153032B
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Abstract
The present invention relates to an extraction technology of effective components in Chinese medicine. In particular, the present invention relates to a novel method of using ultrasound to extract an effective component quercetin in ginkgo leaves. The method uses a design program of orthogonal experiment. Based on technical parameters of the generation method, ultrasonic intensity, functional duration, functional method, solvent type, temperature of the system, and the mixing method of ultrasound through screening and extraction process, the fresh ginkgo leaves can be prepared. The ginkgo leaves are dried at a temperature of 60 DEG C for 24 hours and crushed and screened (0.45 Mu m) to be a material. The ginkgo leaves are filled into ethanol solution of 45 percent in an ultrasonic extraction reactor; the material and solution are mixed according to a ratio (Kg: L) of 1 to from 14 to 15. The generation method of ultrasound comprises a simulated audible device of ultrasound and a radialcylindrical vibration transducer. The ultrasonic power is 100W, and the ultrasonic frequency is 28 kHz. In the extraction method, the ultrasound functions once with an interval of 5 minutes; the functional time of ultrasound is 3 minutes; the total extraction time is 32 minutes; the extraction temperature is 45 DEG C; the stirring method is an air-lift stirring way. With the methods, the extraction rate of the effective component quercetin in ginkgo leaves can reach 96.28 percent.
Description
One, technical field
The present invention relates to Chinese medicine extracts active ingredients new technology, be specifically related to utilize the novel method of effective constituent Mongolian oak skin rope in the ultrasonic extraction Ginkgo Leaf.
Two, background technology
The chemical sense of extracting is usefulness The suitable solvent and appropriate means, with the molten leaching from specimen material as far as possible completely of needed component, avoids or reduce the proposition of unwanted component or other impurity.From plant, extract chemical ingredients as medicinal, existing nearly 5000 history in motherland's traditional medicine.Traditional extraction process can be divided into two big classes according to leaching process to the difference of energy requirement mode.One class is a heating extraction, mainly contains different modes such as decocting extraction method, reflux extraction and soxhlet extraction; Another kind of is to soak extraction method, mainly contains two kinds of dipping extraction method and diacolation extraction methods.Traditional extraction process exist extraction time long, technology is complicated, efficient is low, industrial pollution is big and target compound extracted defective such as non-selectivity.
The ultrasonic extraction technology is to utilize ultrasonic vibration to increase the perviousness that solvent enters vegetable cell, strengthens the rate of mass transfer of extract between two-phase medium.Ultrasonic vibration can also produce cavitation bubble, and bubble strenuous exercise pair cell forms powerful shearing force, forms local assault ripple and high-speed jet effect when particularly bubble collapses, and vegetable cell is broken, and quickens the release of entocyte.Ultrasonic extraction can carry out (20-60 ℃) in lower temperature range, can effectively prevent purpose composition degraded in the plant, and extract is had high selectivity, and extraction time is short, and extract purity and percentage extraction are all higher.The large-scale industrial production process is less to the investment of ultrasonic extraction process reforming equipment from traditional extraction technique.Ultrasonic extraction will become most important core technology means in the traditional Chinese medicine extraction process.
(Extraction Ginkgo biloga EGB) uses existing several centuries as treatment to Folium Ginkgo extract, has become one of the most normal ethical goods of opening of doctor in Germany and France.Quercetin is one of main effective constituent in the Semen Ginkgo extrac (structural formula is seen Fig.1), multiple biological activity is arranged, have preferably eliminate the phlegm, antitussive action, bring high blood pressure down, strengthen capillary resistance, reduce effects such as capillary fragility, reducing blood-fat, coronary artery dilator, coronary blood flow increasing, antitumor action, anticoagulant, the clinical chronic bronchitis that is used for the treatment of has the assisting therapy effect to coronary heart disease and hyperpietic.High efficiency extraction to Quercetin is that Ginkgo Leaf is as one of medicinal main approach.This patent is exactly the effective solution that proposes at this problem.
Three, summary of the invention
The present invention adopts the orthogonal experiment design, by the technical parameters such as ultrasonic intensity, ultrasonic occurring mode, action time, the mode of action, type of solvent, system temperature and alr mode of screening ultrasonic extraction process, obtain quick, accurate, efficient, economical from Ginkgo Leaf the method for extracting effective components Quercetin.
A kind of method of utilizing active ingredient of meletin in the ultrasonic-assisted extraction Ginkgo Leaf, it is characterized in that step is as follows: with fresh puerarin, through 60 ℃ of oven dry 24h, pulverize, cross 0.45 μ m sieve and be material, in the ultrasonic extraction still of packing into, add 45% ethanol, solid-liquid ratio 1: 14-15Kg/L mixes, under the air lift type agitation condition in 45 ℃ of supersound extraction 32min, ultrasonic occurring mode: the ultrasonic sound-producing device of independent-excited, radial vibration column transverter, ultrasonic power are 100W, and ultrasonic frequency is 28kHz, extracting mode is that 5min is once ultrasonic at interval, each 3min, quercetin content adopts high-efficient liquid phase chromatogram technique analysis in the extracting solution, and chromatographic column is Varian, Microsorb-MV100C18, detect wavelength 360nm, moving phase is methanol=55: 45, and phosphoric acid is transferred pH=2.5; Column temperature is 25 ℃, and flow velocity is 1.0ml/min, and sample size is 10 μ l, and the reference material external standard method is quantitative.
The present invention adopts method to compare with existing extractive technique, has the following advantages and distinguished effect:
1. do not need high temperature, energy consumption is low: the optimum temps of ultrasonic extraction Chinese medicinal materials is 20 ℃-60 ℃, and effective constituent in the medicinal material of case of thermal instability, facile hydrolysis or oxidation is had provide protection, can not destroy the physiologically active of thermo-sensitivity medicine.Simultaneously, can cut down the consumption of energy greatly.
2. shorten extraction time: the intensified by ultrasonic wave traditional Chinese medicine extraction can obtain the optimum extraction rate at 20-40min usually, and extraction time can shorten more than 8-10 times than traditional method, has improved device processes amount and efficient.
3. wide adaptability: the ultrasonic extraction Chinese medicinal materials is not subjected to the restriction of medicinal material effective constituent polarity, molecular weight size etc., is applicable to the extraction of many types Chinese medicinal materials effective constituent.
4. it is few to extract soup impurity, and effective constituent is easy to separate, purifying.
5. operation is simple, and equipment maintenance is convenient.The large-scale industrial production process is less to the investment of ultrasonic extraction process reforming equipment from traditional extraction technique.
Four, embodiment
The best-of-breed technology scheme that obtains is:
With the fresh gingko blade, through 60 ℃ of oven dry 24h, pulverize, sieve (0.45 μ m) is material.Pack into and add 45% aqueous ethanolic solution in the ultrasonic extraction still, (Kg: L) 1: 14-15 mixes solid-liquid ratio.Ultrasonic occurring mode: the ultrasonic sound-producing device of independent-excited, radial vibration column transverter; Ultrasonic power: 100W; Ultrasonic frequency: 28kHz; Extracting mode: 5min at interval, ultrasonication once, ultrasonication time 3min, total extraction time 32min; Extract temperature: 45 ℃; Alr mode: air lift type stirs; According to aforesaid method, the active ingredient of meletin extraction yield can reach 96.28% in the Ginkgo Leaf.
Claims (1)
1. denomination of invention: a kind of method of utilizing Quercetin in the ultrasonic extraction Ginkgo Leaf
The present invention relates to Chinese medicine extracts active ingredients new technology, be specifically related to utilize the novel method of active ingredient of meletin in the ultrasonic extraction Ginkgo Leaf.This method is to adopt the orthogonal experiment design, on the basis by technical parameters such as ultrasonic wave occurring mode, ultrasound intensity, action time, the mode of action, type of solvent, system temperature and alr mode in the screening extraction process, obtain with the fresh gingko blade, through 60 ℃ of oven dry 24h, pulverize, sieve (0.45 μ m) is material.Pack into and add 45% aqueous ethanolic solution in the ultrasonic extraction still, (Kg: L) 1: 14-15 mixes solid-liquid ratio.Ultrasonic occurring mode: the ultrasonic sound-producing device of independent-excited, radial vibration column transverter; Ultrasonic power: 100W; Ultrasonic frequency: 28kHz; Extracting mode: 5min at interval, ultrasonication once, ultrasonication time 3min, total extraction time 32min; Extract temperature: 45 ℃; Alr mode: air lift type stirs; According to aforesaid method, the active ingredient of meletin extraction yield can reach 96.28% in the Ginkgo Leaf.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2006101525394A CN101153032B (en) | 2006-09-28 | 2006-09-28 | Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave |
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| CN2006101525394A CN101153032B (en) | 2006-09-28 | 2006-09-28 | Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave |
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| Publication Number | Publication Date |
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| CN101153032A CN101153032A (en) | 2008-04-02 |
| CN101153032B true CN101153032B (en) | 2010-09-08 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985439B (en) * | 2010-11-18 | 2012-05-09 | 桂林市三棱生物制品有限公司 | Process for separating and extracting 98% quercetin from aboveground part of pubescent holly root |
| CN103374053A (en) * | 2012-04-12 | 2013-10-30 | 新疆农业大学 | Method for extracting cyclic adenosine monophosphate from red dates by ultrasonic waves |
| CN104402854A (en) * | 2014-11-12 | 2015-03-11 | 重庆市畜牧科学院 | Method for extracting quercetin from mulberry leaves by utilizing ultrasonic waves |
| CN109010267B (en) * | 2018-09-17 | 2019-06-11 | 海南卓泰制药有限公司 | A kind of paclitaxel injection |
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2006
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Non-Patent Citations (4)
| Title |
|---|
| 刘海鹏.超声波/回流法银杏叶总黄酮提取的研究.化学工程师2005 8.2005,2005(8),49-50. * |
| 张玉祥.银杏叶超声波提取工艺研究.时珍国医国药17 5.2006,17(5),784.-785. * |
| 王延峰.超声法提取银杏叶黄酮的研究.食品科学23 8.2002,23(8),166-167. * |
| 钱大玮.邳州银杏叶黄酮类成分分析.时珍国医国药15 7.2004,15(7),387-388. * |
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