CN101153032A - Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave - Google Patents
Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave Download PDFInfo
- Publication number
- CN101153032A CN101153032A CNA2006101525394A CN200610152539A CN101153032A CN 101153032 A CN101153032 A CN 101153032A CN A2006101525394 A CNA2006101525394 A CN A2006101525394A CN 200610152539 A CN200610152539 A CN 200610152539A CN 101153032 A CN101153032 A CN 101153032A
- Authority
- CN
- China
- Prior art keywords
- extraction
- ultrasonic
- meletin
- active ingredient
- ultrasound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to an extraction technology of effective components in Chinese medicine. In particular, the present invention relates to a novel method of using ultrasound to extract an effective component quercetin in ginkgo leaves. The method uses a design program of orthogonal experiment. Based on technical parameters of the generation method, ultrasonic intensity, functional duration, functional method, solvent type, temperature of the system, and the mixing method of ultrasound through screening and extraction process, the fresh ginkgo leaves can be prepared. The ginkgo leaves are dried at a temperature of 60 DEG C for 24 hours and crushed and screened (0.45 Mu m) to be a material. The ginkgo leaves are filled into ethanol solution of 45 percent in an ultrasonic extraction reactor; the material and solution are mixed according to a ratio (Kg: L) of 1 to from 14 to 15. The generation method of ultrasound comprises a simulated audible device of ultrasound and a radial cylindrical vibration transducer. The ultrasonic power is 100W, and the ultrasonic frequency is 28 kHz. In the extraction method, the ultrasound functions once with an interval of 5 minutes; the functional time of ultrasound is 3 minutes; the total extraction time is 32 minutes; the extraction temperature is 45 DEG C; the stirring method is an air-lift stirring way. With the methods, the extraction rate of the effective component quercetin in ginkgo leaves can reach 96.28 percent.
Description
One, technical field
The present invention relates to Chinese medicine extracts active ingredients new technology, be specifically related to utilize the novel method of active ingredient of meletin in the ultrasonic extraction Ginkgo Leaf.
Two, background technology
The chemical sense of extracting is usefulness The suitable solvent and appropriate means, with the molten leaching from specimen material as far as possible completely of needed component, avoids or reduce the proposition of unwanted component or other impurity.From plant, extract chemical ingredients as medicinal, existing nearly 5000 history in motherland's traditional medicine.Traditional extraction process can be divided into two big classes according to leaching process to the difference of energy requirement mode.One class is a heating extraction, mainly contains different modes such as decocting extraction method, reflux extraction and soxhlet extraction; Another kind of is to soak extraction method, mainly contains two kinds of dipping extraction method and diacolation extraction methods.Traditional extraction process exist extraction time long, technology is complicated, efficient is low, industrial pollution is big and target compound extracted defective such as non-selectivity.
The ultrasonic extraction technology is to utilize ultrasonic vibration to increase the perviousness that solvent enters vegetable cell, strengthens the rate of mass transfer of extract between two-phase medium.Ultrasonic vibration can also produce cavitation bubble, and bubble strenuous exercise pair cell forms powerful shearing force, forms local assault ripple and high-speed jet effect when particularly bubble collapses, and vegetable cell is broken, and quickens the release of entocyte.Ultrasonic extraction can carry out (20-60 ℃) in lower temperature range, can effectively prevent purpose composition degraded in the plant, and extract is had high selectivity, and extraction time is short, and extract purity and percentage extraction are all higher.The large-scale industrial production process is less to the investment of ultrasonic extraction process reforming equipment from traditional extraction technique.Ultrasonic extraction will become most important core technology means in the traditional Chinese medicine extraction process.
(Extraction Ginkgo biloga EGB) uses existing several centuries as treatment to Folium Ginkgo extract, has become one of the most normal ethical goods of opening of doctor in Germany and France.Quercetin is one of main effective constituent in the Semen Ginkgo extrac (structural formula is seen Fig.1), multiple biological activity is arranged, have preferably eliminate the phlegm, antitussive action, bring high blood pressure down, strengthen capillary resistance, reduce effects such as capillary fragility, reducing blood-fat, coronary artery dilator, coronary blood flow increasing, antitumor action, anticoagulant, the clinical chronic bronchitis that is used for the treatment of has the assisting therapy effect to coronary heart disease and hyperpietic.High efficiency extraction to Quercetin is that Ginkgo Leaf is as one of medicinal main approach.This patent is exactly the effective solution that proposes at this problem.
Three, summary of the invention
The present invention adopts the orthogonal experiment design, by the technical parameters such as ultrasonic intensity, ultrasonic occurring mode, action time, the mode of action, type of solvent, system temperature and alr mode of screening ultrasonic extraction process, obtain quick, accurate, efficient, economical from Ginkgo Leaf the method for extracting effective components Quercetin.
The present invention adopts method to compare with existing extractive technique, has the following advantages and distinguished effect:
1. do not need high temperature, energy consumption is low: the optimum temps of ultrasonic extraction Chinese medicinal materials is 20-60, and effective constituent in the medicinal material of case of thermal instability, facile hydrolysis or oxidation is had provide protection, can not destroy the physiologically active of thermo-sensitivity medicine.Simultaneously, can cut down the consumption of energy greatly.
2. shorten extraction time: the intensified by ultrasonic wave traditional Chinese medicine extraction can obtain the optimum extraction rate at 20-40min usually, and extraction time can shorten more than 8-10 times than traditional method, has improved device processes amount and efficient.
3. wide adaptability: the ultrasonic extraction Chinese medicinal materials is not subjected to the restriction of medicinal material effective constituent polarity, molecular weight size etc., is applicable to the extraction of many types Chinese medicinal materials effective constituent.
4. it is few to extract soup impurity, and effective constituent is easy to separate, purifying.
5. operation is simple, and equipment maintenance is convenient.The large-scale industrial production process is less to the investment of ultrasonic extraction process reforming equipment from traditional extraction technique.
Four, embodiment
The best-of-breed technology scheme that obtains is:
With the fresh gingko blade, through 60 ℃ of oven dry 24h, pulverize, sieve (0.45 μ m) is material.Pack into and add 45% aqueous ethanolic solution in the ultrasonic extraction still, (Kg: L) 1: 14-15 mixes solid-liquid ratio.Ultrasonic occurring mode: the ultrasonic sound-producing device of independent-excited, radial vibration column transverter; Ultrasonic power: 100W; Ultrasonic frequency: 28kHz; Extracting mode: 5min at interval, ultrasonication once, ultrasonication time 3min, total extraction time 32min; Extract temperature: 45 ℃; Alr mode: air lift type stirs; According to aforesaid method, the active ingredient of meletin extraction yield can reach 96.28% in the Ginkgo Leaf.
Claims (5)
1. utilize the method for active ingredient of meletin in the ultrasonic-assisted extraction Ginkgo Leaf to have the extraction efficiency height, solvent load is few, quick and stable characteristics.Its principle is to utilize ultrasonic vibration to increase the perviousness that solvent enters vegetable cell, strengthens the rate of mass transfer of extract between vegetable cell and extraction medium.In addition, ultrasonic vibration produces cavitation bubble and forms shearing force, and formation local assault ripple and high-speed jet can make vegetable cell break during the bubble collapse, quicken the release of entocyte.By the orthogonal experiment design, select and optimize ultrasonic intensity, occurring mode, action time, type of solvent and composition, system temperature and alr mode etc. to extract parameters among the present invention, improved target compound extraction efficiency and selectivity.Present method operation steps is: with the fresh gingko blade, through 60 ℃ of oven dry 24h, pulverize, sieve (0.45 μ m) is material.Packing in the ultrasonic extraction still, (ultrasonic wave traditional Chinese medicine extraction instrument: the ningbo of china state pharmaceutical equipment Manufacturing Co., Ltd of shaking, model: TCLX200), add 45% ethanol, (Kg: L) 1: 14-15 mixes solid-liquid ratio.Ultrasonic occurring mode: the ultrasonic sound-producing device of independent-excited, radial vibration column transverter; Ultrasonic power: 100W; Ultrasonic frequency: 28kHz; Extracting mode: 5min at interval, ultrasonication once, ultrasonication time 3min, total extraction time 32min; Extract temperature: 45 ℃; Alr mode: air lift type stirs; Quercetin content adopts high performance liquid chromatography (high performance liquid chromatograph: German KNAUER, model: SY-400K) analyze in the extracting solution.Chromatographic column Varian, Microsorb-MV100C18 (4.6 * 250nm, 5 μ m); Detect wavelength 360nm; Moving phase: methanol-water=55: 45, phosphoric acid is transferred pH=2.5; Column temperature: 25 ℃, flow velocity: 1.0ml/min; Sample size: 10 μ l; The reference material external standard method is quantitative, retention time 6.438min.According to aforesaid method, the active ingredient of meletin extraction yield can reach 96.28% in the Ginkgo Leaf.
2. the method for utilizing active ingredient of meletin in the ultrasonic extraction Ginkgo Leaf according to claim 1, its ultrasonic occurring mode condition flag is the ultrasonic sound-producing device of independent-excited, radial vibration column transverter.
3. the method for utilizing active ingredient of meletin in the ultrasonic extraction Ginkgo Leaf according to claim 1, it extracts the material condition and is characterized as fresh puerarin, through 60 ℃ of oven dry 24h, pulverizes, and (0.45 μ m) sieves.
4. the method for utilizing active ingredient of meletin in the ultrasonic extraction Ginkgo Leaf according to claim 1, its extracting method condition flag is ultrasonic power: 100W; Ultrasonic frequency: 28kHz; Extracting mode: 5min at interval, ultrasonication once, ultrasonication time 3min, total extraction time 32min; Extract temperature: 45 ℃; Alr mode: air lift type stirs.
5. the method for utilizing active ingredient of meletin in the ultrasonic extraction Ginkgo Leaf according to claim 1, its extraction efficiency are characterized as that the active ingredient of meletin extraction yield can reach 96.28% in the Ginkgo Leaf.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101525394A CN101153032B (en) | 2006-09-28 | 2006-09-28 | Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101525394A CN101153032B (en) | 2006-09-28 | 2006-09-28 | Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101153032A true CN101153032A (en) | 2008-04-02 |
| CN101153032B CN101153032B (en) | 2010-09-08 |
Family
ID=39254938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006101525394A Expired - Fee Related CN101153032B (en) | 2006-09-28 | 2006-09-28 | Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101153032B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985439A (en) * | 2010-11-18 | 2011-03-16 | 桂林市三棱生物制品有限公司 | Process for separating and extracting 98% quercetin from aboveground part of pubescent holly root |
| CN103374053A (en) * | 2012-04-12 | 2013-10-30 | 新疆农业大学 | Method for extracting cyclic adenosine monophosphate from red dates by ultrasonic waves |
| CN104286034A (en) * | 2014-09-26 | 2015-01-21 | 浙江大学宁波理工学院 | Preparation method of hickory nut epicarp extract |
| CN104402854A (en) * | 2014-11-12 | 2015-03-11 | 重庆市畜牧科学院 | Method for extracting quercetin from mulberry leaves by utilizing ultrasonic waves |
| CN109010267A (en) * | 2018-09-17 | 2018-12-18 | 海南卓泰制药有限公司 | A kind of paclitaxel injection |
-
2006
- 2006-09-28 CN CN2006101525394A patent/CN101153032B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985439A (en) * | 2010-11-18 | 2011-03-16 | 桂林市三棱生物制品有限公司 | Process for separating and extracting 98% quercetin from aboveground part of pubescent holly root |
| CN103374053A (en) * | 2012-04-12 | 2013-10-30 | 新疆农业大学 | Method for extracting cyclic adenosine monophosphate from red dates by ultrasonic waves |
| CN104286034A (en) * | 2014-09-26 | 2015-01-21 | 浙江大学宁波理工学院 | Preparation method of hickory nut epicarp extract |
| CN104402854A (en) * | 2014-11-12 | 2015-03-11 | 重庆市畜牧科学院 | Method for extracting quercetin from mulberry leaves by utilizing ultrasonic waves |
| CN109010267A (en) * | 2018-09-17 | 2018-12-18 | 海南卓泰制药有限公司 | A kind of paclitaxel injection |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101153032B (en) | 2010-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101205232A (en) | Technical new method for extracting artemisinin from sweet wormwood plants by ultrasonic assistance | |
| CN101597296B (en) | Novel method for efficiently extracting and producing artemisinin by ionic liquid | |
| CN110240580B (en) | A method for preparing Saviae Miltiorrhizae radix extract with eutectic solvent | |
| CN101152267B (en) | Method of preparing anticold grain based on ultrasound wave auxiliary extraction technology | |
| CN101585885A (en) | Method for preparing polygonatum odoratum polysaccharide | |
| CN102517144A (en) | Method for extracting oil and procyanidin from grape seeds | |
| CN102432582A (en) | Preparation method of proanthocyanidin | |
| CN107353183A (en) | A kind of method that high-content of resveratrol is extracted from giant knotweed | |
| CN101153032B (en) | Method for abstracting active ingredient of meletin in folium ginkgo with ultrasound wave | |
| CN102048655B (en) | Method for extracting asparagus polysaccharides from discarded asparagus and application of asparagus polysaccharides | |
| CN101152222A (en) | Method for extracting total flavone from folium ginkgo with ultrasound wave assisted | |
| CN100425247C (en) | Refining ganoderam lucidum spore oil and method for preparing same | |
| CN104844584A (en) | Method for extracting puerarin from residue of radix puerariae | |
| Liu et al. | Ultrasonic extraction and purification of scutellarin from Erigerontis Herba using deep eutectic solvent | |
| CN101153033B (en) | Method for abstracting active ingredient of kaempferol in folium ginkgo with ultrasound wave | |
| CN101153034B (en) | Method for abstracting active ingredient of Isorhamnetin in folium ginkgo with ultrasound wave | |
| CN102614342B (en) | Extract of Lemai Keli pharmaceutical materials and method for producing same | |
| CN101757105B (en) | Extraction method capable of enriching fat soluble and water soluble parts of salvia simultaneously | |
| CN103058979B (en) | Method for extracting procyanidine in ginkgo leaves | |
| CN104987341A (en) | Method for extracting artemisinin from sweet wormwood | |
| CN101773528B (en) | Preparation method of total terpene lactones with low phenolic acid | |
| CN107652258A (en) | Quercetin, the method for isoquercitrin are extracted from beggar-ticks | |
| CN107556277A (en) | Quercetin, the method for isoquercitrin are extracted from hyperici,herba | |
| CN107582593A (en) | The method that general flavone is extracted from beggar-ticks | |
| CN101780213B (en) | Method for preparing ophiopogan total saponies |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100908 Termination date: 20100928 |