CN101139550A

CN101139550A - Polymerase interlocking reaction system, power supply module used therefore, heating module and physical container

Info

Publication number: CN101139550A
Application number: CNA2006101290647A
Authority: CN
Inventors: 王敬顺; 王明中
Original assignee: Advanced Connectek Inc
Current assignee: Advanced Connectek Inc
Priority date: 2006-09-06
Filing date: 2006-09-06
Publication date: 2008-03-12

Abstract

The invention relates to a polymerase chain reaction (PCR) system, a power supplying module, heating module and a specimen container for the system. The PCR system comprises a circuit board and a controller that is electrically connected with the conductive path of the circuit board and for controlling the reaction condition for a DNA specimen to carry out PCR. The invention is characterized in that the power supplying module is electrically connected with the conductive path of the circuit board; the heating module is located on the power supplying module and receives the power supply from the power supplying module to generate heat energy; the specimen container is for holding DNA specimen and can be located removably on the heating module; at positioning, the heat energy from the heating module is received so that the DNA specimen carries out PCR; by the modular design of the PCR system, main components are easy to draw out or replace.

Description

Polymerase chain reaction system, the employed power supply module of system, heat module and physical container

[technical field]

The present invention relates to a kind of doctor's reactive system, the employed power supply module of system, heat module and physical container of giving birth to, particularly relate to a kind of modular polymerase chain reaction system, the employed power supply module of system, heat module and physical container.

[background technology]

In recent years,, in many different fields, significant development is arranged, especially give birth to doctor's check and analysis fast and also played the part of crucial pushing hands role at microminiaturization along with the maturation of micro electronmechanical process technique.Give birth to doctor's detection wafer by the microfluid that micro electronmechanical process technique is produced, it has high detection usefulness, jettisonable, portability, low corpse or other object for laboratory examination and chemical testing consumption, low power consuming, volume is little and low cost and other advantages.Especially to integrate the design of omnidistance microfluid system on same wafer, tool development potentiality and marketable value.

Polymerase chain reaction (Polymerase Chain Reaction is called for short PCR) is very important in recent years DNA (Deoxyribonucleic acid, DNA) multiplication technique.It is by temperature controlling with dna double spiral sex change (Denaturation), bind (Annealing), (Extension) three steps that extend, the dna sequence dna of demarcating (target sequence) is amplified to millions of times.

From beginning in 1993, for polymerase chain reaction give birth to doctor's reactive system in countries in the world existing considerable research deliver out.Majority is to utilize dual mode to carry out at present, and a kind of is corpse or other object for laboratory examination and chemical testing movable type, and it controls several FX temperature, cooperates loop parameter, and a corpse or other object for laboratory examination and chemical testing is moved in each temperature control district.Another kind is the temperature cycle formula, and it is that a corpse or other object for laboratory examination and chemical testing is fixed in the zone, cooperates loop parameter, changes the temperature of conversion zone.

The above-mentioned doctor's wafer of giving birth to is done proliferative response at the virus and the bacterium that with DNA are substrate mostly, yet, different corpse or other object for laboratory examination and chemical testing are subject to operate improperly or various pollution causes the reaction failure in the same system operation, therefore need a cover to settle and take out a corpse or other object for laboratory examination and chemical testing easily, and can optionally change and give birth to doctor's wafer and integral construction livings doctor simple, that safeguard easily system, allow the use that the personage of medical circles can feel at ease and the mortality of reduction biochemical reaction.

[summary of the invention]

Because above-mentioned problem, the inventor finds that physical container belongs to running stores, droppable, and structure should be simply, price should be cheap, and the placement before reaction and reacted taking-up should be very convenient; And the needed temperature of polymerase chain reaction system can be reached by power supply module and a module of heating, the power supply module can be played the leading role in by the electric connector of technology maturation, and the module of heating can be by electrically connecting through the electric connector of an electric connector with the power supply module with the made doctor's wafer of giving birth to of semiconductor technology.

A purpose of the present invention is that a kind of modular polymerase chain reaction system, the employed power supply module of system, heat module and physical container are being provided.

Polymerase chain reaction of the present invention system comprises the control device that a circuit card and electrically connects the conductive path on the circuit card, and control device is in order to control the reaction conditions that a DNA corpse or other object for laboratory examination and chemical testing carries out polymerase chain reaction (PCR) program.

It is characterized in that: described polymerase chain reaction system also comprises: a power supply module, be with circuit card on conductive path electrically connect; One module of heating is to be positioned to power on the module and to accept the power supply of power supply module to produce heat energy; And a physical container, be take up a DNA corpse or other object for laboratory examination and chemical testing and the module that is positioned removedly to heat on, be to accept to heat heat energy that module produced during the location so that a DNA corpse or other object for laboratory examination and chemical testing carries out polymerase chain reaction.

Beneficial effect of the present invention is: by the modularized design of aforesaid polymerase chain reaction system, the employed power supply module of system, heat module and physical container, make that major parts of the present invention is easy to extract or change.

[description of drawings]

Fig. 1 is a part of sectional view, and the preferred embodiment of polymerase chain reaction of the present invention system is described;

Fig. 2 is a system block diagrams, illustrates that preferred embodiment carries out the employed control device of polymerase chain reaction;

Fig. 3 is a three-dimensional exploded view, illustrates that the power supply module of preferred embodiment comprises two first electric connectors, and each first electric connector is being fixed on the circuit card separately;

Fig. 4 is a three-dimensional exploded view, illustrates that first electric connector of preferred embodiment has one first insulation shell and many first conducting terminals;

Fig. 5 is a three-dimensional exploded view, and the module of heating that preferred embodiment is described comprises cures wafer, one second electric connector and a metal base all one's life;

Fig. 6 is a three-dimensional exploded view, illustrates that second electric connector of preferred embodiment comprises one second insulation shell and many second conducting terminals;

Fig. 7 is a stereographic map, illustrates that the doctor's wafer of giving birth to of preferred embodiment has a substrate and forms four conversion zones that structure is identical;

Fig. 8 is a three-dimensional exploded view, illustrates that the physical container of preferred embodiment comprises a loam cake, a substrate and a sheet glass;

Fig. 9 is a three-dimensional exploded view, illustrates that preferred embodiment assembling physical container is on the module of heating;

Figure 10 is a stereographic map, illustrates that the physical container in the preferred embodiment and the module of heating finished the state of assembling;

Figure 11 is a sectional view, when the fastener that preferred embodiment is described is switched to erect position, and second conducting terminal electric connection of each first conducting terminal of first electric connector and second electric connector.

[embodiment]

The present invention is described in detail below in conjunction with drawings and Examples:

In advance explanation is, this preferred embodiment is be example explanation with polysaccharase chain (PCR) reaction, and right notion of the present invention is also applicable for other similar biochemical reaction, is not restriction with the polymerase chain reaction.

Consult Fig. 1, in the preferred embodiment of the present invention, polymerase chain reaction system 100 one carries out the system of polymerase chain reaction for a DNA corpse or other object for laboratory examination and chemical testing, its have a circuit card 10, be installed on power supply module 1 on the circuit card 10, one with control device 2, a physical container 3, a heat sink 4 and of the circuit card 10 electric connections module 5 of heating.

Power supply module 1 be with circuit card 10 on conductive path electrically connect; Control device 2 is the conductive paths that electrically connect on the circuit card 10, in order to control the reaction conditions that a DNA corpse or other object for laboratory examination and chemical testing carries out the polymerase chain reaction program; The module 5 of heating is to be positioned to power on the module 1 and to electrically connect with conductive path on the circuit card 10, and conductive path control power supply module 1 power supply that energising back control device 2 sees through on the circuit card 10 gives the module 5 of heating so that the module 5 of heating produces heat energy; Physical container 3 be take up a DNA corpse or other object for laboratory examination and chemical testing and the module 5 that is positioned removedly to heat on, be to accept to heat heat energy that module 5 produced during the location so that a DNA corpse or other object for laboratory examination and chemical testing carries out polymerase chain reaction.

Dna replication dna is polymerase chain reaction (the polymerasechain reaction that utilizes biotechnology, PCR), principle is to add designed introduction (primer) in a DNA corpse or other object for laboratory examination and chemical testing, the sequence of this introduction is that segmental two terminal sequences of the desired propagation of foundation design, and two ends toward each other, can be not bonding with the dna double stock.Add the ferment buffered soln (buffer) and DNA synthetic raw material of DNA polymerizing enzyme (polymerase) and proper concn, just can carry out the nucleic acid proliferative response.The propagation step comprises three temperature operation districts.The DNA that at first is double stranded helix can separate, and becomes sub-thread structure (95 degree Celsius approximately), then is that the introduction refining is closed, and introduction can combine with the DNA of sub-thread, prepares to duplicate (55 degree Celsius approximately); Final step then is that introduction extends, and just carries out the prolongation of introduction and synthetic (70 degree Celsius approximately) of another strand under the effect of DNA NOS.Noted earlier is the circulation of a temperature, and through a circulation, the number of DNA can double, if therefore through N circulation, then has 2 Nth power DNA number doubly.

In this preferred embodiment, control device 2 is reaction conditionss that a control DNA corpse or other object for laboratory examination and chemical testing carries out the polymerase chain reaction program, import module 23 design temperatures and cycle index and watching relevent information by the user at demonstration module 24, the lifting that can carry out differing temps by the variation of regulating and controlling temperature is to carry out sex change, bonding, extension three steps to DNA, with three steps is that a circulation is carried out repeatedly, sets cycle index and then can make the quantity of DNA be the geometric ratio increase.

Consult Fig. 2, control device 2 comprises that a control unit 21, an omnibus bar interface 22, an input module 23 and show module 24.

Input module 23 and demonstration module 24 be 22 electric connection control units 21 by the omnibus bar interface, and input module 23 is available for users to import information and sets its reaction conditionss (as: temperature, heating and cooling time etc.) with command control unit 21; Show that 24 of modules are in order to show relevant message.

Consult Fig. 1 and Fig. 3, power supply module 1 in this preferred embodiment, comprises two first electric connectors 11, being fixed on the circuit card 10 and electrically connecting with conductive path on the circuit card 10 separately.

Consult Fig. 3 and Fig. 4, first electric connector 11 has one first insulation shell 111 and many first conducting terminals 112, first insulation shell 111 is a hexahedron, comprise a roof 1111, a diapire 1112, an antetheca 1113, a rear wall 1114 and left and right sides wall 1115, between top, diapire 1111,1112, a plurality of the first terminal storage tanks 1110 that run through top, diapire 1111,1112 are arranged.Near the projecting upwards of rear wall 1114 place's one, baffle plate 113 one side forward is provided with the block 1131 of a wedge shape to one axial baffle plate 113 from the roof 1111 of first insulation shell 111.Two opposite side walls 1115 at first insulation shell 111 is provided with a lug 114 respectively near diapire 1112 places, and lug 114 has a through hole 1140.

Consult Fig. 3, many first conducting terminals 112, be located at respectively in the first terminal storage tank 1110, each root first conducting terminal 112 comprises a fixed part 1121, one from the weld part 1122 of fixed part 1121 to side extension, one from the elastic portion 1123 of fixed part 1121 to opposite side (away from weld part 1122) bending extension continuously (like as small intestine), and an own elasticity portion 1123 ends are to the contact part 1124 that extends away from weld part 1122 directions, when described first conducting terminal 112 is axial respectively when being fixed in the first terminal storage tank 1110 of a correspondence, the part of described contact part 1124 is stretched out (comprising head end) outside the roof 1111 of first insulation shell 111, and the part of described weld part 1122 can be stretched out (comprising tail end) outside the diapire 1112 of first insulation shell 111.

In this preferred embodiment, be provided with two groups, every group six roots of sensation in first electrical connection 11, amount to 12 first conducting terminals 112.Power supply module 1 has two first electric connectors 11, so power supply module 1 has 24 first conducting terminals 112 in respect of four group group, every group six roots of sensation, total.

Consult Fig. 4, the through hole 110 that corresponding respectively first conducting terminal, 112 positions are provided with a plurality of correspondences on the circuit card 10 penetrates for the weld part 1122 of one first conducting terminal 112 respectively, so that with welding process first conducting terminal 112 is produced electrically connects with circuit card 10, because this is a prior art, therefore repeat no more.For being familiar with the people of related art techniques, also first conducting terminal 112 can being changed and adopt surface mounting technique (SMT) and be electrically connected on the circuit card.Simultaneously, lug 114 through holes 1140 places of corresponding first insulation shell 111 also are provided with the screw 120 of a correspondence on the circuit card 10, when first electric connector 11 is installed on the circuit card 10, can pass the screw 120 of a lug 114 and a correspondence by two spiral shells 12 respectively, with being fixed on the circuit card 10 of first electric connector, 11 screw locks.

First electric connector 11 still can further comprise a fastener 13 that is hubbed on first insulation shell 111.Be respectively equipped with a protruded stigma 1116 on the two side 1115 of first insulation shell 111, fastener 13 is made by the insulation material, be U font body, intermediate plate 132 with two side arms 131 and a connection two side arms 131, be respectively equipped with the protruded stigma 1116 on the sidewall 1115 of a pivoted hole 1310 corresponding first insulation shells 111 on the two side arms 131, when the pivoted hole 1310 of fastener 13 when protruded stigma 1116 on the sidewall 1115 combines, fastener 13 can pivot between an obliquity and a upright position with respect to first electric connector 11.When fastener 13 during in obliquity, the intermediate plate 132 of fastener 13 is switched to rear wall 1114 rears of first electric connector 11, and the two side arms 131 of fastener 13 is support with lug 114, makes fastener 13 be unlikely over-tilting backward.The antetheca 1113 of first insulation shell 111 reaches left respectively near roof 1111 places and extends a stop block 1117 that exceeds sidewall 1115 to the right, when fastener 13 during in erect position, top and roof 1111 that the intermediate plate 132 of fastener 13 is switched to first electric connector, 11 roofs 1111 keep at a certain distance away, the two side arms 131 of fastener 13 is subjected to stopping of stop block 1117, fastener 13 is unlikely continues to dump forward again.

Two first electric connectors 11 being installed on the circuit card 10 separately, after the installation, the configuration of two first electric connectors 11 is that the antetheca 1113 of two insulation shells 111 is face-to-face.11 of two first electric connectors a heat sink 4 is set, in present embodiment, heat sink 4 is one to be fixed in the scatterer (heat sink) on the circuit card 10, and scatterer can use commercially available pattern.

Consult Fig. 5, the module 5 of heating comprises cures wafer 51, one second electric connector 52 and a metal base 53 all one's life.

Consult Fig. 6, second electric connector 52 comprises one second insulation shell 521 and many second conducting terminals 523, second insulation shell 521 is a rectangular body, has a length to reaching a short, second insulation shell 521 is provided with a middle hollow bulb 5210, the both sides of hollow bulb 5210 connect with two parallel mouldings 524 to being respectively a body 522, two bodies 522 along long, form the hollow bulb 5210 that is interposed in 522 of two bodies.

The same side of each body 522 is provided with a plurality of along the long second terminal storage tank 5220 to configuration, can ccontaining one second conducting terminal 523 in each second terminal storage tank 5220, each root second conducting terminal 523 comprises a fixed part 5231, one from the touching portion 5232 of fixed part to side extension, reach one from the contact part 5233 of fixed part to opposite side (away from touching portion 5232) extension, when described second conducting terminal 523 is individually fixed in the second terminal storage tank 5220 of a correspondence, the part (comprising free end) of the touching portion 5232 of each second conducting terminal 523 and the part (comprising free end) of contact part 5233 expose to outside the second terminal storage tank of being stayed in 5220, contiguous second insulation shells 521 of the touching portion of exposing 5232 long to ora terminalis (minor face of rectangular body), contact part 5233 contiguous second insulation shell, 521 hollow bulbs 5210 that expose.

Consult Fig. 3 and Fig. 6, in present embodiment, second conducting terminal, 523 numbers that are arranged in the body 522 are equal to first conducting terminal, 112 numbers set in one first electric connector 11, second conducting terminal 523 in one body 522 is provided with two groups, every group six roots of sensation, amounts to 12, in respect of four group group, every group six roots of sensation, total 24 second conducting terminals 523 is arranged in second electric connector 52.Each root second conducting terminal 523 corresponding one first conducting terminal 112, in other words when power supply module 1 electrically connects second electric connector 52, the touching portion 5232 of each root second conducting terminal 523 can contact with contact part 1124 head ends of corresponding first conducting terminal 112, after this names a person for a particular job and is described in.

Consult Fig. 7, the doctor's wafer 51 of giving birth to of present embodiment has one by glass or quartz or the made substrate 511 of macromolecular material.Contact part 5233 for corresponding four second conducting terminal 523 groups, give birth on doctor's wafer 51 and form four conversion zones 541～544 that structure is identical, in each conversion zone 541～544, deposit six platinum (Pt) layers separately on prior to substrate, on platinum layer, deposit the pin 511～516 of six gold (Au) material then, pin 512,513 is connected, pin 515,516 is connected, and all the other do not address what be connected is spaced apart.The resistance of gold is little, and the effect of transmission electrical signal is good, and the resistance of platinum is big, and is splendid as heating material.Be provided with a sensing unit 520 in each conversion zone 541～544 simultaneously.

Pin 512,513 constitutes one first galvanic circle, and pin 515,516 constitutes one second galvanic circle, and pin 511 and pin 514 are connected with two ends of sensing unit 520 respectively.

Second galvanic circle that first galvanic circle that pin 512,513 constitutes and pin 515,516 constitute is in order to feed electric current, and each galvanic circle has one respectively and is relative semicircular heating unit 530, each heating unit 530 is platinum layer zones (but not depositing gold layer) of initial deposition, because the heats of platinum is good, feeds electric current and just can produce heat energy rapidly.

Sensing unit 520 is a superfine platinum wire between two heating units 530, is when initial deposition, to make (no gold layer), and mainly be to utilize resistance-type temperature sensor (Resistance Temperature Detectors; RTD) principle, principle are to utilize metallic resistance variation meeting along with the proportional relation of temperature variation, its formula Δ R=α Δ T, and the α value is the standard temperature coefficient of platinum, the change Δ R of resistance value is proportional along with temperature variation Δ T.

According to aforementioned principles, in the resistance change of pin 511 and pin 514 monitoring sensing unit 520 (platinum wire) after wherein a pin feeds electric current, can be with the variation of perception actual temperature as the follow-up basis for estimation that heats or stop to heat of whether continuing.

Mandatory declaration be, in other embodiments, sensing unit 520 can be a thermistor also, being not limited to is platinum wire, knows related art techniques person when coming in addition conversion according to above-mentioned principle.

Consult Fig. 5 again, metab 53 is a U-shaped body in this example, can be fixed on the long limit of second electric connector 52 by dual-side 531, and holds/support living doctor's wafer 51 with the inner face of bottom 532.When heating module 5 combinations, be that life doctor wafer 51 is placed on the metab 53, metab 53 is fixed on second electric connector 52 again, make living doctor's wafer 51 each conversion zone 541～544 be positioned at hollow bulb 5210 places of second electric connector 52, and each pin 511～516 of living doctor's wafer 51 and second conducting terminal, 523 contact parts 5233 of second electric connector 52 are electrically connected.Because giving birth to doctor's wafer 51 can the temperature adjustment when System Operation, for shortening the time of cooling, in this example metab 53 is arranged on the heat sink 4, will give birth to the temperature reduction of doctor's wafer 51 by metab 53 and contacting of heat sink 4 (Fig. 4).Yet, metab 53 and if giving birth to of being supported 51 of doctor's wafers are gapped can to lower heat conducting effects, be this kind situation of preventing, can use heat radiation paster or thermal grease between wafer 51 and the metab 53, give birth to the space of curing wafer 51 and metab 53 to fill up living curing.

Consult Fig. 8, physical container 3 comprises a loam cake 31, by polydimethylsiloxane (PDMS) made a room 32 and a sheet glass 33.

PDMS is a hydrophobic polymer material, but plasticity-is high and high-temperature sterilization, and light transmission is good, and has excellent biological compatibility and elasticity.

Consult Fig. 8 and Fig. 9, loam cake 31 is inverted U-shaped, have an end face 311, two plumbing arms 312 and a bottom surface 313, bottom surface 313 is arranged with a storage tank 314 that can hold room 32, loam cake 31 and room 32 correspondences offer conversion zone 541～544 on four through holes 310,320 and the corresponding respectively doctor's of the giving birth to wafer 51 respectively, combine to the bottom surface of room 32 and sheet glass 33 watertightnesses so that reaction soln splashes into through hole 320 interior unlikely outflows; During physical container 3 combinations, sheet glass loam cake 31 covers on room 32 (this moment room 32 and sheet glass 33 driving fits), and room 32 proper frames are fixed in the storage tank 314 of loam cake 31 bottom surfaces 313.

Consult Fig. 9,10 and 11, during assembling, to heat earlier power supply module 1 combination on module 5 and the circuit card is fastened on by the block 1131 of first electric connector 11 on the button hole 5230 of body 522 of second electric connector 52, and module 1 can be positioned the module 5 of heating to power.

After the button hole 5230 of each body 522 of the block 1131 of each first electric connector 11 and second electric connector 52 fastens, each block 1131 can be executed a downward reactive force, so each second conducting terminal, 523 touching portion 5232 of second electric connector 52 just with each contact part 1124 electric connection of outer corresponding first conducting terminal 112 of the roof 1111 that stretches out first insulation shell 111, this moment, the elastic portion 1123 of first conducting terminal 112 can provide appropriate elastic force, made the conducting terminal 112 of winning keep excellent electrical property to be connected each other with second conducting terminal 523.

In addition, the contact part 5233 of second conducting terminal 523 is when finishing the assembling of the module 5 of heating, electrically connect with the pin 511～516 (Fig. 7) of giving birth on doctor's wafer 51, the electrical signal that each pin 511～516 is required, for example: a wherein pin of pin 511 and pin 514 feeds electric current and finishes a loop through another pin of pin 511 and pin 514 again through sensing unit 520 (platinum wire), can monitor the resistance change of sensing unit 520, pin 512,513 wherein a pin feed electric current and pass through pin 512 again, another pin of 513 is finished first galvanic circle, and pin 515,516 wherein a pin feed electric current and pass through pin 515 again, another pin of 516 is finished second galvanic circle and is produced heat energy or the like in order to make heating unit 530, all is to transmit 511～516 palpuses of each pin through first conducting terminal 112 and second conducting terminal 523.

After two first electric connectors, 11 each button hole 5230 with block 1131 and each body 522 fasten, physical container 3 is placed on the module 5 of heating, four through holes 310 of loam cake 31 and four through holes 320 of room 32 are in alignment with each other, and two plumbing arms, 312 inboards of physical container 3 are cross-placed on the two long sides outside of second electric connector 52.The user can be switched to erect position with fastener 13, makes the intermediate plate 132 of each fastener 13 snap onto on the end face 311 of loam cake 31 to finish all assemblings.Because room 32 is that the PDMS material has elasticity, can bear the pressure that each fastener 13 is applied to loam cake 31, and and then the sheet glass 33 of bottom is fitted with giving birth to doctor's wafer 51, help giving birth to the thermal energy conduction that the heating unit 530 of doctor's wafer 51 produced and give a DNA corpse or other object for laboratory examination and chemical testing (figure does not show).

The principle of operation and the step of polymerase chain reaction process are described as follows:

Consult Fig. 9 and Figure 10, before carrying out polymerase chain reaction, finish the assembling of all parts earlier, add the DNA corpse or other object for laboratory examination and chemical testing of archaeal dna polymerase and introduction (primer) then, archaeal dna polymerase is to be used for the carrying out of catalytic dna replication reaction, makes reaction rate accelerates; Introduction is one section specific few nucleotide sequence, with certain fragment complementation in the dna sequence dna, is used for starting the building-up reactions of DNA; A DNA corpse or other object for laboratory examination and chemical testing is that the through hole 310 through loam cake 31 splashes into the through hole 320 of room 32 and stopped by sheet glass 33.

Consult Fig. 2, control principle with a circuit block diagram explanation control device 2 of simplifying: the user sees through input module 23 and sets controlled variable (temperature, time and predetermined cycle index), be transferred to control unit 21 via omnibus bar interface 22, control unit 21 shows that just relevent information is showing on the module 24, and the controlled variable that control unit 21 configures according to the user begins, and outward current (can be with reference to figure 7 to the heating unit 530 of giving birth to doctor's wafer 51 stage by stage, Fig. 2 only shows one) begin to heat, and why the sensor signal of reception sensing unit 520 (can be with reference to figure 7, Fig. 2 only shows) is to judge actual temperature.When control unit 21 reception sensor signals judged that temperature is lower than preset temperature, then control unit 21 outward currents produced heat energy to make heating unit 530; On the other hand, when control unit 21 receives sensor signal judgement temperature above preset temperature, then outward current is not stopping heating for control unit 21, and heat sink 4 can make and give birth to doctor's wafer 51 fast coolings to save another temperature cycle required cooling time this moment.

Conclude above-mentionedly, polymerase chain reaction of the present invention system, the employed power supply module of system, heat module and physical container have following advantage:

1. design of modularization makes that major parts of the present invention is easy to extract or change.

2. physical container 3 can remove easily or be installed in the polymerase chain reaction system 100, makes things convenient for user's operation when experiment.

3. because physical container 3 is to take up a corpse or other object for laboratory examination and chemical testing to be infected with easily, therefore can be along with each experiment is abandoned after finishing, avoid reducing the mortality of experiment because of being polluted by a DNA corpse or other object for laboratory examination and chemical testing, reusable as for remaining component because not contacting a DNA corpse or other object for laboratory examination and chemical testing so free of contamination anxiety, allow the reliability of experiment greatly increase.

4. first electric connector 11 is chimeric with the button hole 5230 of each body 522 of the block 1131 and second electric connector 52, cooperate room 32 to provide appropriate elastic force order to give birth to doctor's wafer 51 and can fit each other, help to give birth to the thermal energy conduction that the heating unit 530 of doctor's wafer 51 produced and give a DNA corpse or other object for laboratory examination and chemical testing with the room 32 that the bottom is provided with sheet glass 33.

Claims

1. a polymerase chain reaction system comprises the control device that a circuit card and electrically connects the conductive path on the circuit card, and control device may command one a DNA corpse or other object for laboratory examination and chemical testing carries out the reaction conditions of polymerase chain reaction (PCR) program; It is characterized in that: described polymerase chain reaction system also comprises:

One power supply module is with the conductive path electric connection on the circuit card;

One module of heating electrically connects on the module that is positioned to power and with conductive path on the circuit card, accepts the power supply of power supply module to produce heat energy; And

One physical container, in order to take up a DNA corpse or other object for laboratory examination and chemical testing and the module that is positioned removedly to heat on, be to accept to heat heat energy that module produced during the location so that a DNA corpse or other object for laboratory examination and chemical testing carries out polymerase chain reaction.

2. polymerase chain reaction as claimed in claim 1 system is characterized in that: described polymerase chain reaction system also comprises:

One heat sink, it is fixed on the circuit card and with the module bottom of heating and contacts.

3. polymerase chain reaction as claimed in claim 1 system, it is characterized in that: described power supply module comprises two first electric connectors and is electrically connected at a circuit card at intervals, and each first electric connector comprises:

One first insulation shell comprises a roof, a diapire, an antetheca, a rear wall, left and right sides wall, and a plurality of the first terminal storage tank that runs through top, diapire; And

Many first conducting terminals, be located at respectively in the described the first terminal storage tank, each root first conducting terminal comprises a fixed part, one from the weld part of fixed part to side extension, one from fixed part to the elastic portion that bends extension away from the opposite side of weld part continuously, and one own elasticity portion end to the contact part that extends away from the weld part direction, when each first conducting terminal is axial respectively when being fixed in the corresponding the first terminal storage tank, described contact part comprises that the part of head end stretches out outside the roof of first insulation shell, and described weld part comprises that the part of tail end stretches out outside the diapire of first insulation shell.

4. polymerase chain reaction as claimed in claim 3 system is characterized in that:

Described first electric connector is provided with an axial baffle plate at first insulation shell,

Near the projecting upwards of rear wall place one, and baffle plate is provided with the block of a wedge shape to baffle plate towards the one side of antetheca from the roof of first insulation shell.

5. polymerase chain reaction as claimed in claim 3 system is characterized in that:

Described first electric connector is provided with a lug in the two opposite side walls of first insulation shell respectively near the diapire place, lug has a through hole, the lug through hole place of corresponding first insulation shell also is provided with the screw of a correspondence on the circuit card, when first electric connector is installed on the circuit card, can pass lug and corresponding screw respectively by two-screw, with the circuit card that is fixed on of the first electric connector screw lock.

6. polymerase chain reaction as claimed in claim 3 system is characterized in that:

Described first electric connector also has a fastener that is hubbed on first insulation shell.

7. polymerase chain reaction as claimed in claim 6 system is characterized in that:

Described first electric connector also is respectively equipped with a protruded stigma on the two side of first insulation shell, fastener has the intermediate plate that two side arms and connects two side arms, be respectively equipped with the protruded stigma on the sidewall of corresponding first insulation shell of a pivoted hole on the two side arms, when the pivoted hole of fastener when protruded stigma on the sidewall combines, fastener can pivot between an obliquity and a upright position with respect to first electric connector.

8. polymerase chain reaction as claimed in claim 7 system is characterized in that:

Described first electric connector reaches left respectively near the roof place at the antetheca of first insulation shell and extends a stop block that exceeds sidewall to the right, when fastener during in erect position, top and roof that the intermediate plate of fastener is switched to the first electric connector roof keep at a certain distance away, the two side arms of fastener is subjected to stopping of stop block, fastener is unlikely dumps forward.

9. polymerase chain reaction as claimed in claim 1 system, it is characterized in that: the described module of heating comprises:

Cure wafer all one's life, has a substrate, forms at least one conversion zone on the substrate;

One second electric connector comprises:

One second insulation shell can hold and give birth to doctor's wafer, has a length to reaching a short, and the edge is long to the second terminal storage tank that is provided with a plurality of configurations; And

Many second conducting terminals, comprise that respectively a fixed part, is from the touching portion of fixed part to side extension, reach one from the contact part of fixed part away from the opposite side extension of touching portion, in the time of in it is placed in one second terminal storage tank, each touching portion, contact part all expose; And

One metab is located at a side of second junctor.

10. polymerase chain reaction as claimed in claim 9 system is characterized in that:

Second insulation shell of described second electric connector is provided with a middle hollow bulb, the both sides of hollow bulb are along growing to being respectively a body, the same side of each body is provided with the described second terminal storage tank, two bodies connect with two parallel mouldings, form the hollow bulb that is interposed between two bodies.

11. polymerase chain reaction as claimed in claim 10 system is characterized in that:

The second conducting end subnumber of described second electric connector is equal to the first set conducting end subnumber in the power supply module, when the power supply module electrically connected second electric connector, the touching portion of each root second conducting terminal can contact with corresponding first conducting terminal.

12. polymerase chain reaction as claimed in claim 10 system is characterized in that:

Described second conducting terminal of described second electric connector is individually fixed in the second terminal storage tank of a correspondence, the touching portion of each second conducting terminal comprises that a free-ended part and contact part comprise that a free-ended part exposes to outside the second terminal storage tank of being stayed in, the contiguous second insulation shell length of the touching portion of exposing to ora terminalis, the contiguous second insulation shell hollow bulb of the contact part that exposes.

13. the employed power supply module of polymerase chain reaction system comprises two first electric connectors and is electrically connected at a circuit card at intervals, it is characterized in that: described each first electric connector comprises:

14. the employed power supply module of polymerase chain reaction system as claimed in claim 13,

It is characterized in that:

Described first electric connector is provided with an axial baffle plate at first insulation shell, and near the projecting upwards of rear wall place one, and baffle plate is provided with the block of a wedge shape to baffle plate towards the one side of antetheca from the roof of first insulation shell.

15. the employed power supply module of polymerase chain reaction system as claimed in claim 13,

It is characterized in that:

16. the employed power supply module of polymerase chain reaction system as claimed in claim 13,

It is characterized in that:

17. the employed power supply module of polymerase chain reaction system as claimed in claim 16,

It is characterized in that:

18. the employed power supply module of polymerase chain reaction system as claimed in claim 17,

It is characterized in that:

Described first electric connector reaches left respectively near the roof place at the antetheca of first insulation shell and extends a stop block that exceeds sidewall to the right, when fastener during in erect position, top and roof that the intermediate plate of fastener is switched to the first electric connector roof keep at a certain distance away, the two side arms of fastener is subjected to stopping of stop block, fastener is unlikely continues to dump forward again.

19. the employed module of heating of polymerase chain reaction system, it is characterized in that: the described module of heating comprises:

One second electric connector comprises one second insulation shell, can hold and give birth to doctor's wafer, has a length to reaching a short, and the edge is long to the second terminal storage tank that is provided with a plurality of configurations; And many second conducting terminals, comprise that respectively a fixed part, is from the touching portion of fixed part to side extension, reach one from the contact part of fixed part away from the opposite side extension of touching portion, in the time of in it is placed in one second terminal storage tank, each touching portion, contact part all expose; And

One metab is located at a side of second junctor.

20. the employed module of heating of polymerase chain reaction system as claimed in claim 19,

It is characterized in that:

21. the employed module of heating of polymerase chain reaction system as claimed in claim 20,

It is characterized in that:

22. the employed module of heating of polymerase chain reaction system as claimed in claim 20,

It is characterized in that:

23. the employed module of heating of polymerase chain reaction system as claimed in claim 19,

It is characterized in that:

The described contact part that is provided with described second conducting terminal of a sensing unit and corresponding second electric connector in the conversion zone of curing wafer of giving birth to is formed with a plurality of pins.

24. the employed module of heating of polymerase chain reaction system as claimed in claim 23,

It is characterized in that:

It is prior to depositing a plurality of platinum layers separately on the substrate, deposit the pin of a plurality of gold materials afterwards on platinum layer that the described pin of the described doctor's of giving birth to wafer is made.

25. the employed module of heating of polymerase chain reaction system as claimed in claim 24,

It is characterized in that:

Totally six of the described pins of described living doctor's wafer, wherein two constitute one first galvanic circles, and other two pins constitute one second galvanic circle, remain two pins and then are connected with two ends of sensing unit respectively.

26. the employed module of heating of polymerase chain reaction system as claimed in claim 25,

It is characterized in that:

Described first and second galvanic circle of giving birth to doctor's wafer has one respectively and is relative semicircular heating unit, and sensing unit is arranged between the two semicircular heating units.

27. the employed module of heating of polymerase chain reaction system as claimed in claim 26,

It is characterized in that:

Described sensing unit of giving birth to doctor's wafer feeds the resistance change of electric current with the monitoring sensing unit at two termination pin of sensing unit between two heating units, with the variation of perception actual temperature as the follow-up basis for estimation that heats or stop to heat of whether continuing.

28. the employed module of heating of polymerase chain reaction system as claimed in claim 19,

It is characterized in that:

Described metab is a U-shaped body, can be fixed on the long limit of second electric connector by dual-side, and holds living doctor's wafer with the inner face of bottom.

29. the employed physical container of polymerase chain reaction system, it is characterized in that: described physical container comprises:

One loam cake;

One room, (PDMS) is made by polydimethylsiloxane, is positioned at a side of loam cake, and room and loam cake offer at least one corresponding through hole simultaneously; And

One sheet glass, with the side watertightness ground that does not contact loam cake of room in conjunction with so that splash into the unlikely outflow of at least one through hole internal reaction solution.

30. the employed physical container of polymerase chain reaction system as claimed in claim 29 is characterized in that:

Described loam cake is inverted U-shaped, has an end face, two plumbing arms and a bottom surface, and the bottom surface is arranged with a storage tank that can hold room.