CN101132803A - Tissue engineering edible meat and method of manufacturing the same - Google Patents
Tissue engineering edible meat and method of manufacturing the same Download PDFInfo
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- CN101132803A CN101132803A CNA2004800440171A CN200480044017A CN101132803A CN 101132803 A CN101132803 A CN 101132803A CN A2004800440171 A CNA2004800440171 A CN A2004800440171A CN 200480044017 A CN200480044017 A CN 200480044017A CN 101132803 A CN101132803 A CN 101132803A
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Abstract
A non-human tissue engineered meat product and a method for producing such meat product are disclosed. The meat product comprises muscle cells that are grown ex vivo and is used for food consumption. The muscle cells may be grown and attached to a support structure and may be derived from any non-human cells. The meat product may also comprise other cells such as fat cells or cartilage cells, or both, that are grown ex vivo together with muscle cells.
Description
Technical field
The present invention relates to produce and obtain edible meat product.More specifically, the invention relates to tissue engineering edible meat (tissue engineered meat for consumption).
Background technology
Meat product such as beef, Carnis Sus domestica, Carnis caprae seu ovis, poultry or fish are welcome products in the food consumption.At present, meat product all is to take from animal, and this production method is poor efficiency very, because a big chunk of whole grains is used to letting animals feed in the agricultural production, rather than by human edible.For example, in the U.S., feed stripped accounts for 70% of whole Semen Tritici aestivis, corn and other grain-production.In addition, in order to produce one pound of beef, feed stripped is drunk and planted to animal needs thousands of pounds water.Simultaneously, according to statistics, the whole world surpasses 800,000,000 people's malnutrition, and 50,000 people is arranged by hungry to death every day.
Present meat production method also can endanger environment.Tropical rain forest reduces to cultivate the speed that one pound of beef approximately need consume 500 square feet of Tropical rain forests.Similarly, it is very effective to be used to fish for halobiontic state-of-the-art technology, so that ocean and lake are all by overfishing.Once very common species have frequently faced extinction now or have become extinct.
For addressing these problems, the emphasis of research work is to concentrate on the efficient that improves breeding or cultivation domestic animal at present.For example, use growth hormone to accelerate the domestic animal growth, can consume less grain and water like this.Growth hormone is injected into domestic animal usually, but has also developed new growth hormone introducing method, and it adopts technique for gene engineering such as transgenic animal or cloned animal.Yet present meat production method still needs water, grain and soil to come stock raising.
Another problem of present meat production method is the food pollution problem.On an average, the annual U.S. that causes because of some thing of taking in is once sick per capita and 9000 people's death are arranged.In order to control food pollution, the present strategy of government is to check meat in the course of processing.Yet the farm that United States Department of Agriculture (USDA) and Food and Drug Administration seldom regulate and control to produce this type of pathogen is because they lack the power of regulating and control the farm.Yet except dust cloth Salmonella (E.coli0156:H7), other dangerous antibacterial is thought " inherent " in the raw meat legally.Yet, wherein two kinds---arc bacillus and Salmonellas---in " intrinsic antibacterial " account for all diseases that cause by edible meat and poultry 80% and all death 75%.
For example it is reported that in the check of the Salmonella of domestic fowl farming, about 25% broiler and 45% garden chicken are positive.Center for Disease Control (CDC) estimates that the 70-90% of all chickens has infected the arc bacillus.The arc bacillus infection causes cramp, dysentery disease and fever.In the U.S., annual arc bacillus infection causes about 800 people's death.The infection of arc bacillus also may cause Green-barre syndrome, and this disease need be provided special care to several weeks of treatment.Along with the generation of more antibiotic resistant bacterial strain, will increase more by these bacterial serious diseases and dead occurrence frequency.This makes many scientists begin to use antibiotic to produce query as domestic animal feed additive to continuing.
Therefore, need provide a kind of, more healthy eating meat product production method more effective, safer than existing production method.
Summary of the invention
The method that the invention relates to the tissue engineering edible meat product and be used to produce this meat product.In a kind of specific embodiment, the muscle cell that meat product of the present invention comprises external (ex vivo) cultivates.These muscle cells can be cultivated and are attached on the supporting construction, and the myocyte can be derived from any non-human cell.In a kind of preferred specific embodiment, meat product of the present invention pollutes away from any harmful microorganism or parasite fully.The another kind of specific embodiment of the present invention relates to a kind of meat product that comprises muscle cell and other cell such as adipose cell and/or chondrocyte, these other cells with muscle cell in vitro cultivation.In another specific embodiment, meat product of the present invention comprises the muscle cell that once is exposed to electric current or oscillating current (electric or oscillatingcurrent).
The specific embodiment
Usually, meat product is taken from the muscle of animal.The butcher correspondingly cuts beef, poultry, Carnis caprae seu ovis, fish or Carnis Sus domestica so that as sales such as beefsteak, pigeon chest dried meat, sliced mutton, slice of fish, pork chops.Meat product also comprises the derivatives of meat product, as being processed into meat ball, hamburger patty, fish pill, sausage, salami (salami), boulogna sausage (bologna), Petaso etc.Meat product can also comprise muscular tissue or by the meat of air-dry or drying shrinkage, as dried beef.
A kind of specific embodiment of the present invention comprises a kind of method of producing edible meat product.This method can be included in external (in vitro) cultivates muscle stem cell, and at external (ex vivo) these cell differentiations is become concrete muscle cell type, as skeletal muscle cell or smooth muscle cell.Muscle cell can derive from any by human edible non-human animal, as mammal (for example cattle, Babalus bubalis L., pig, sheep, deer etc.), birds (for example chicken, duck, ostrich, turkey, pheasant etc.), Fish (for example sailfish, salmon, tuna, jewfish, Squaliobarbus ourriculus, Silurus asotus fish etc.), invertebrates (for example Lobster, Eriocheir sinensis, shrimp, clam, Concha Ostreae, mussel, Hemicentrotus seu Strongylocentrotus etc.), reptile (for example Serpentis, crocodile, Chelomia mydas (Linnaeus). etc.) and Amphibian (for example Rana nigromaculata).Preferably, muscle cell derives from the pluripotent embryonic mescenchymal stem cell (pluri-potent embryonic mesenchymal stem cells) that can bear muscle cell, adipose cell, osteocyte and chondrocyte.Muscle cell can also derive from totipotent embryos stem cell (toti-potent embryonic stem cells), as the cell from blastocyte (blastocyte stage), germ cell, Placenta Hominis or the umbilical cord of these animals.
Muscle cell can be cultivated attached to the muscular tissue on the supporting construction in culture medium, as two dimension or three-dimensional scaffold (scaffold) or supporting construction.Muscle cell can be on two-dimentional supporting construction forms the cell that multilamellar is cultivated in as petri diss, and these multi-layer cellulars can be peeled and processing and eating.Other example of two dimension supporting construction can comprise porous septum, and porous septum allows nutrient to be diffused into the opposite side of attached cell from the culture medium of barrier film one side.In this class condition of culture, the culture medium of cellular exposure in the barrier film both sides can be obtained more cellular layer, just, cell had both been accepted the nutrient that comes from the diffusion of a membranous side, also accepted to be covered with the nutrient in the culture medium of the cell of cultivating on the barrier film.
Muscle cell can also be cultivated on three-dimensional supporting construction or around three-dimensional supporting construction and cultivate or cultivate in three-dimensional supporting construction inside.As required, this class supporting construction can be designed to different sizes, profile and form, if desired, the muscle cell of being cultivated is formed and dissimilar corresponding profile of muscular tissue and forms, as beefsteak, (cattle, sheep etc.) tenderloin, shin, pigeon chest dried meat, drumsticks, sliced mutton, slice of fish, lobster tail etc.Supporting construction can be made by natural or synthetic biomaterial, preferably selects nontoxic material for use, even so that their can not bring harm when being eaten yet.For example, natural biologic material can comprise collagen protein, fibronectin (fibronectin), laminine (laminin) or other extracellular matrix.For example, the synthesising biological material can comprise hydroxyapatite, alginate, Polyethylene Glycol acid (polyglycolic acid), polylactic acid (polylactic acid) or their copolymer.Supporting construction of the present invention can be designed to solid or semi-solid the support.
In order to obtain best cell and tissue cultivating, preferably, supporting construction of the present invention has higher porosity, so that the surf zone of maximum is provided for cell attachment.In a kind of three-dimensional supporting construction, it can be molded into and comprise a branch vascular network (branched vascular network), so that send nutrient and the metabolism product of these cells is discharged to the cell of meat product inside.In this specific embodiment, it is edible adopting above-mentioned nontoxic branch vascular network natural or that the synthesising biological material is made.In addition, this supporting construction also may comprise adherent peptide (adhesionpeptides), cell adhesion molecule or with this supporting construction other somatomedin covalently or non-covalently to connect.The example of peptide comprises the aminoacid sequence as Arg-Gly-Asp or Arg-Glu-Asp-Val.See also Niklason, people such as L exist
Transplantation immunology5 (4): deliver among the 303-306 (1997): the organizational project progress of blood vessel or its hetero-organization.Herein as a reference with the full text of the document.
On the other hand, the condition of culture of these muscle cells can comprise static, condition of culture that stir or dynamic flow.In order to improve output significantly, preferable methods is to adopt bioreactor, and it can produce a large amount of cells, and can control nutrient, gas, metabolism product preferably and regulate flowing of molecule.In addition, bioreactor can provide physics and signal machinery, as compression, so that irritation cell and produce specific biomolecule.See Vacanti, people such as J exist
Lancet354 Suppl.1 deliver among the pSI32-34 (1999): organizational project: the design and the structure of the live body displacement apparatus that remakes and transplant of being used to perform the operation.Herein as a reference with the full text of the document.
In another embodiment of the invention, meat product derives from the muscle cell of in vitro cultivation, and it can comprise the adipose cell that derives from any non-human animal.The fertile common taste of meat is relatively good, but it has higher fat content, and dangers to health are bigger, as heart disease.Therefore, the ratio of muscle cell and adipose cell can be regulated in vessel, so that produce the meat product with optimum taste and health effect.This adjusting can be finished with the ratio of adipose cell by the muscle cell of the initial plantation of control (seeded) in culture medium, and/or the concentration of somatomedin that muscle cell or adipose cell is worked by change as required or differentiation factor (differentiation factors) and ratio change and finish.
In another embodiment of the invention, the cartilage that derives from chondrocyte can at first form end supporting layer or the structure that combines with supporting construction.Then, muscle cell and/or adipose cell can be planted on the chondrocyte layer.The interaction of muscle cell and chondrocyte can further provide tissue to form required necessary conditioning signal.Example with meat product of muscle cell and chondrocyte comprises pigeon chest dried meat or pork chop.
In a kind of preferred implementation of the present invention, can adopt aseptic technique to cultivate the muscle cell of final formation meat product, so that meat product is substantially free of harmful microorganism, as antibacterial, fungus, virus, Protein virus, protozoacide or their combination.Detrimental microorganisms can comprise the pathological form microorganism, as Salmonella, arc bacillus, dust cloth Salmonella E.coli_0156:H7 etc.In addition, the muscle cell of cultivating in culture medium can be substantially free of parasite, and such as cestode, it can infect the muscle of all animals, and when the edible meat that does not fully boil of the mankind and be transferred to the mankind.When leaving the biological production line, packing when meat product, also can aseptic technique.Such quality assurance can adopt the standard test method at microorganism or chemicals well known in the prior art to monitor." be substantially free of " and be meant that microorganism or parasitic concentration are lower than pollutant clinical meaning level, just, even be lower than in case take in the level that will cause disease or health risk.
In another preferred embodiment of the present invention, the meat product that derives from the muscle cell of in vitro cultivation can be exposed to electric current or oscillating current.Be different from from the muscular tissue on the animal health muscular tissue that external or vessel are cultivated never to move (for example always not being used to mobile lower limb).Thereby, the muscle cell in the vessel, muscular tissue or meat product are exposed to electric current or oscillating current, can echokinesis, make that the meat of in vitro cultivation is more similar on quality to the meat on the animal health.Electric current or oscillating current can also improve muscle cell growth in vitro speed.The muscle cell that electric current or oscillating current can be applied in muscle stem cell or go out from differentiation of stem cells.
In another embodiment of the invention, can add other nutrient that meat product lacked that comes from the animal health such as vitamin etc., to improve the nutritive value of meat product of the present invention.This both can realize by directly increase nutrient in cultivating base, also can realize by genetic engineering.For example, can be in the muscle cell of cultivating transfection be used for the enzyme gene of synthetic specified vitamin (as vitamin D, A or different vitamin B complexs), to generate specified vitamin.
In another embodiment of the invention, regulatory factor, somatomedin or other gene prod also can be incorporated in the muscle cell by gene technology.Known these are called as myogenicity regulatory factor (myogenic regulatory factors, abbreviation MRFs) the factor, can stimulate and regulate the growth of muscle in the organism, but generally, in the organism or the muscle cell in the vessel do not produce these factors.Therefore, by in the muscle cell of cultivating, expressing the myogenicity regulatory factor, can improve the output of muscle cell in the vessel.
In another embodiment of the invention, derive from that the meat product of muscle cell comprises different meat product derivants in the vessel.For example, these derivants can be by making meat paste or chopping with the muscular tissue of cultivating in the vessel, and mix suitable flavoring agent, makes meat ball, fish pill, hamburger patty etc.For example, this derivant can also be made dried beef, Petaso, boulogna sausage, salami etc. by muscle cell being cut stratification and adding spice.Therefore, meat product of the present invention can be used for being processed to form the food of any kind that animal flesh can be made into.
Following embodiment has disclosed those skilled in the art and how to have used the present invention to produce meat product in vessel.Cytobiology, cell culture and immunohistochemistry technology are not described in this article clearly, because they were reported in scientific and technical literature fully.
Embodiment 1
How present embodiment has described the separating multipotent mescenchymal stem cell, to be used for producing meat product at vessel.Mescenchymal stem cell can produce muscle cell, adipose cell, osteocyte and chondrocyte.Mescenchymal stem cell can be separated from the section of the embryonal tissue of any inhuman animal embryo.For example, on one's body cattle, the embryo's mesenchymal tissue that is rich in the multipotency muscle stem cell is preferably at the 30th day to 40 days or earlier separate from the embryo.After cutting apart or cutting into slices, embryonal tissue can be chopped into the small pieces that size is approximately 1mm * 1mm in pH value is 7.45 phosphate-buffered salt (being called for short PBS).Five to ten chopped being organized among the 300 μ l PBS that contain 0.25% insulin and 0.1%EDTA were hatched 30 minutes in 37 ℃ of mild down stirrings.Then, this tissue can be deposited on the bottom of test tube by gravity or slight centrifugalize.Then, take the supernatant that comprises insulin/EDTA solution away, and be replaced into the 300 μ l PBS that contain 0.1% collagenase, handled 10 to 30 minutes at 37 ℃ again.As required, collagenase digesting can be repeated to circulate several times.Depend on the viscosity (being derived from the DNA that ruined cell discharges) of solution, between twice circulation, can in collagenase solution, add the PBS that 40 μ l contain 1mg/ml DNase (deoxyribonuclease).
This reaction can stop by adding medium, as DMEM or Ham ' s F-12, perhaps both mixture of 1: 1 are (available from Life Technologies, Inc., the Rockville, the Maryland State), and the L-glutaminate of the Hepes of additional 10mM, 2mM (Sigma-Aldrich), the heat-inactivated cattle fetal blood of 10-20% are clear or Ox blood serum (Hyclone laboratory, Lip river root, Utah), the penicillin of 100 units/ml and the streptomycin (" perfect dielectric ") of 100 μ g/ml.Inhale up and down lightly and move (pipetting) these tissues, adopt centrifugal separator in perfect dielectric, to wash these cells once or twice then, cell can be dissociated fully.Then cell can be arranged to suitable size, can apply in natural biologic material (for example collagen, fibronectin, laminine or other extracellular matrix) or synthesising biological material (for example hydroxyapatite, alginate, Polyethylene Glycol acid, polylactic acid or their copolymer) or both petri disses, cultivate and with 5% CO at 37 ℃
2Carry out balance.
Embodiment 2
After mescenchymal stem cell was separated, in order to obtain into muscle cell (myoblasts) or muscle stem cell, they can carry out enrichment in culture medium.At first, as embodiment 1 described dissociate and wash after, cell can be carried on respectively in the different petri disses.Adopt 60 millimeters petri diss, cell can at first be hatched two to four hours in perfect dielectric.In this process, epithelial cell will often tend to be attached on the petri diss apace, and sarcoplast still is retained in the supernatant.Collect supernatant then, sarcoplast can be carried on the different petri diss that is coated with the natural or synthesising biological material of apposition, those biomaterials of mentioning as embodiment 1.Sarcoplast can have somatomedin such as the Skeletal Muscle Growth factor by replenishing, prostaglandin F
2 α(be called for short PGF
2 α) and the culture medium of insulin-like growth factor I (be called for short IGF-I) and by enrichment.
Further, sarcoplast can by in perfect dielectric, cultivate or in less medium (as lacking fetal bovine serum) than perfect dielectric cultivate and specific growth or the differentiation factor of additional muscle, be the PGF of 24pg/ml as concentration range to 28pg/ml
2 αAnd 10
-6M to 10
-5The insulin of M, and be divided into specific muscle cell.In order the most closely to imitate the intravital muscle cell of animal, it is arranged by neurocyte usually, can also replenish suitable neurotransmitters in the culture medium, as acetylcholine.
Embodiment 3
Perhaps, sarcoplast can carry out enrichment from the totipotent embryos stem cell.Totipotent cell can be taken from the stem cell in fertilised non-human eggs, umbilical cord or the Placenta Hominis that adopts the external fertilization technology in the vessel or take from the embryonic stem cell of separating (ES) from the blastocyst stage cell.For example, collect the ES cell, leniently dissociate with insulin, and with reconstitution cell leukaemia inhibitory factor (Chemicon, Santiago, California) and feeder cells be detained embryonic fiber archeocyte (growth arrestedembryonic fibroblasts cells) as growth and in vessel, cultivate.These totipotent cells can be handled with somatomedin, as PGF
2 αOr IGF-I, become different sarcoplasts so that lure these cell differentiations into.
Embodiment 4
The immunohistochemistry of employing standard or original position (in-situ) hybridization technique is identified as myocyte or muscle cell.Say that simply sarcoplast of cultivating or muscle cell are transferred on the microscope slide that is coated with the suitable foregoing extracellular matrix of apposition in culture medium.Adopt foregoing condition, these cells are cultivated into required quantity and are broken up.In abundant growth with after differential period, these cells can mix 4% formaldehyde.If adopt intrabody labelling or nucleotide probe, this cell barrier film can permeate 1% trinitrophenol-40 (NP-40) or trinitrotoluene-X (Triton-X).The antibody of sarcoplast or muscle cell specific marker is as can be from Sigma
The myosin, titin, the Alpha-actinine that obtain can be used to discern these cells by the immunohistochemistry's technology that adopts standard.Selectively, RNA or the dna probe that is used for the strand of these labellings also can be used in situ hybridization.
In addition, when muscle cell had been attached on the described three dimensional support structure in back, they can be frozen, cut apart and adopt antibody labeling to discern, as can be from Sigma
The antibody that obtains at myosin, titin, 12101, TnT, Alpha-actinine.
Embodiment 5
Two-dimentional or three-dimensional scaffold or support can be made by natural biomaterial (for example collagen, fibronectin, laminine or other extracellular matrix) or synthetic biomaterial (for example hydroxyapatite, alginate, polyglycolic acid, polylactic acid and their copolymer), are perhaps made with the synthesising biological material by natural biologic material.Preferably, three-dimensional scaffold is manufactured with individual path, to be used to making nutrient and culture medium arrive the muscular tissue inside of this formation.The raw material of these scaffolds and the example of building method are disclosed by following United States Patent (USP): the 5th, 686, No. 091, name is called " the biodegradable foamed materials that is used for cell transplantation "; The 5th, 863, No. 984, name is called " the porous seepage material that comprises the Biostatic of compound bio high polymer "; The 5th, 770, No. 417, name is called " the three-dimensional fiber scaffold that comprises attached cell that is used for producing vascular tissue on animal body "; And the 5th, 916, No. 265, name is called " production method that is used as the biological cell epimatrix of cell seeding scaffold and implantation device ".These patents are here quoted in full with for referencial use.
Supporting construction preferably be manufactured into different sizes, form and form in case the growth that makes muscular tissue corresponding to dissimilar meat products, such as beefsteak, (cattle, sheep etc.) tenderloin, shin, pigeon chest dried meat, drumsticks, sliced mutton, slice of fish, lobster tail etc.
Embodiment 6
Adipose cell, chondrocyte and osteoblast can both break up from multipotency mescenchymal stem cell or totipotent embryos stem cell.These stem cell can be as separated as described in embodiment 1 or 3.These stem cell can DMEM or Ham ' s F12 or above-mentioned both in mixture cultivate at 1: 1.Culture medium can replenish thyroxin, siderophillin, insulin and other somatomedin, as insulin like growth factor (being called for short IGF), basic fibroblast somatomedin and cultivation hormone.
For adipose cell, differentiation can realize by using bone morphogenetic protein (being called for short BMP) to handle stem cell, as BMP-4 and BMP-2, known they be used to bring out adipose cell lines (lineages).See that people such as Ahrens exist
The DNA cytobiologyDeliver among the 12:871-880 (1993): differentiation is introduced in the expression in the leaf origin C 3H10T1/2 cell between Muridae of human bone morphogen protein-2 or protein-4 in visibly different mesenchymal cell blood lineage; People such as Wang exist
SomatomedinDeliver among the 9:57 (1993): bone morphogen protein-2 brings out obligation and differentiation in C3H10T1/2 and 3T3 cell.The full text of quoting these documents here is for referencial use.
Except BMP, the differentiation of adipose cell can also adopt the robust and sturdy anti-agent (agonist of peroxisome proliferator-activated receptorgamma is called for short PPAR gamma) of the active proliferin receptor of peroxisome gamma to strengthen as BRL 49653 (rosiglitazone).See that Sottile and Seuwen exist
FEBS Lett475 (3): deliver among the 201-204 (2000): the fat-based of leaf precursor was because of (adipogenic) differentiation between the bone morphogen protein-2 of cooperating with BRL 49653 (rosiglitazone) stimulated.Herein as a reference with the full text of the document.
In some cases, by adopting long-chain fatty acid (being called for short LCFA) or thiazolidinedione or both to be processed into myocyte or muscle satellite cell together, sarcoplast even can be induced and change differentiation (trans-differentiate) one-tenth lipoblast (adipose cell precursor).People such as Grimaldi, sarcoplast breaks up to the commentaries on classics of adipoblasts: the triggering effect of fatty acid and thiazolidinediones,
Prostaglandin Leukot Essent fatty acid57 (1): 71-75 (1997); People such as Teboul, Thiazolidinediones and fatty acid convert the myosinogen cell to the class adipose cell,
J.Biol. Chem.270 (47): 28183-28187 (1995).The full text of quoting these documents here is for referencial use
Therefore, by with a certain ratio plantation and co-cultivation muscle cell and adipose cell, can produce meat product with desired fat content.Selectively, stem cell allows to be divided at first sarcoplast, and after a while, LCFA or thiadolidinediones were added with variable concentrations and different open-assembly times, so that according to required mode the sarcoplast commentaries on classics is divided into adipose cell.In addition, the growth of muscle cell and adipose cell can be regulated by control growing concentration and differentiation factor.For example,, less BMP factor concentration can be in culture medium, added, the PGF of higher concentration can be added simultaneously if need less adipose cell in the final meat product
2 αAnd/or insulin is to promote the muscle cell growth.
Embodiment 7
Chondrocyte can also obtain from animal knee or thorax separation.Adopt technology similar to Example 1, the tissue of cutting apart from knee or thorax can be by chopping, wash by collagenase digesting and by perfect dielectric.These cells are carried respectively then, so that increase the purity of chondrocyte.
Known chondrocyte can respond mechanical stress and break up.Therefore, preferably, these cells can be accepted the effect of shear flow pressure, as United States Patent (USP) the 5th, 928, No. 945 described, its name is called " applying shear flow pressure to produce cartilage to chondrocyte or cartilage stem cell ", quotes this full patent texts here as a reference.
Chondrocyte can form ground floor at first and support cell in three-dimensional scaffold.Then, sarcoplast and/or adipose cell can be planted on the chondrocyte layer, and are cultivated into required size.Like this, the chondrocyte layer can provide extra support or somatomedin to muscle cell.
Embodiment 8
The muscle cell of cultivating in the vessel is different from the muscle cell of growing up in the animal body, because the intravital muscle cell of animal is used in the process of activity or limb motion.Because the muscle of animal body is used, muscle cell, the muscle cell in the extremity for example is retracted and loosens according to the motion of extremity.Therefore, in order to imitate the growth of the muscle cell on the animal body more approx, the cell of cultivating in vessel can be exposed to electric current or oscillating current, and the perhaps pulse of electric current or oscillating current is so that contract muscles cell.Electron probe can be dipped in the culture medium, so that send light and slow electric current.Selectively, supporting construction can coated conductive material.The example of conductive material and the coating method on supporting construction thereof is by United States Patent (USP) the 5th, 843, discloses for No. 741, and its name is called " changing the method that depends on cell differentiation that supports on conductive polymer ", and the full text of quoting this patent here as a reference.
Aforesaid embodiment has described the method at the produced in vitro meat product.These embodiment just are used to illustrate, rather than limitation of the present invention.Be appreciated that modification and the combination on the basis of these embodiment, carried out, all do not depart from spirit of the present invention.
Claims (23)
1. edible inhuman meat product, it comprises the inhuman muscle cell of in vitro cultivation.
2. inhuman meat product as claimed in claim 1, it further comprises a supporting construction, wherein, described inhuman muscle cell is attached on this supporting construction.
3. inhuman meat product as claimed in claim 1, wherein, described inhuman muscle cell is the skeletal muscle cell.
4. inhuman meat product as claimed in claim 1, wherein, described inhuman muscle cell derives from the animal that is selected from as in next group: mammal, birds, Fish, invertebrates, reptile and Amphibian.
5. inhuman meat product as claimed in claim 1, wherein, described inhuman meat product is substantially free of harmful microorgranic contaminant.
6. inhuman meat product as claimed in claim 1, wherein, described inhuman muscle cell derives from pluripotent cell or totipotent cell.
7. inhuman meat product as claimed in claim 1, wherein, described inhuman muscle cell is exposed in the electric current to be handled.
8. inhuman meat product as claimed in claim 1, it further comprises the inhuman adipose cell of in vitro cultivation.
9. inhuman meat product as claimed in claim 8, wherein, described inhuman adipose cell obtains from inhuman sarcoplast transfer differentiation.
10. inhuman meat product as claimed in claim 8, wherein, described inhuman adipose cell derives from multipotency or all-round inhuman stem cell.
11. inhuman meat product as claimed in claim 1, it further comprises the inhuman chondrocyte of in vitro cultivation.
12. inhuman meat product as claimed in claim 10, wherein, described inhuman chondrocyte is between a supporting construction and described inhuman muscle cell.
13. inhuman meat product as claimed in claim 10, wherein, described inhuman chondrocyte is exposed in the mechanical stress to be handled.
14. a method of producing edible inhuman meat product, it comprises the steps:
The inhuman muscle stem cell of In vitro culture;
Described inhuman muscle stem cell is planted on the supporting construction; And
Cultivate described inhuman muscle stem cell, to produce inhuman meat product.
15. method as claimed in claim 13, wherein, the step of the inhuman muscle stem cell of described cultivation comprises:
Described inhuman muscle stem cell is divided into dissimilar inhuman muscle cells.
16. method as claimed in claim 14, it further comprises:
Described inhuman muscle cell is exposed in electric current or the oscillating current handles.
17. method as claimed in claim 13, it further comprises:
Add nutrient, so that it is incorporated in the described inhuman meat product.
18. method as claimed in claim 13, wherein, described inhuman muscle cell derives from the animal that is selected from as next group: mammal, birds, Fish, invertebrates, reptile and Amphibian.
19. method as claimed in claim 13, wherein, described inhuman meat product is substantially free of harmful microorgranic contaminant.
20. a method of producing edible inhuman meat product, it comprises the steps:
Inhuman muscle cell of external co-cultivation and inhuman adipose cell;
Described inhuman muscle cell and described inhuman adipose cell are planted on the supporting construction; And
Cultivate described inhuman muscle cell and described inhuman adipose cell, to produce inhuman meat product.
21. a method of producing edible inhuman meat product, it comprises the steps:
The inhuman muscle stem cell of In vitro culture;
Described inhuman muscle stem cell is planted on the supporting construction;
Adopt the described inhuman muscle stem cell of fatty acid treatment, so that described inhuman muscle stem cell commentaries on classics is divided into adipose cell; And
Cultivate described adipose cell, to produce inhuman meat product.
22. a method of producing edible inhuman meat product, it comprises the steps:
The inhuman chondrocyte of In vitro culture;
Described inhuman chondrocyte is planted on the supporting construction;
On described supporting construction or around it, inhuman muscle cell is cultivated with described inhuman chondrocyte; And
Cultivate described inhuman muscle cell, to produce inhuman meat product.
23. method as claimed in claim 20, wherein, described inhuman chondrocyte is exposed in the mechanical stress to be handled.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103747693A (en) * | 2011-07-26 | 2014-04-23 | 密苏里大学董事会 | Engineered comestible meat |
| CN106282093A (en) * | 2016-10-08 | 2017-01-04 | 上海生乐康生物技术发展有限公司 | A kind of production method of edible cell |
| CN110730615A (en) * | 2017-04-09 | 2020-01-24 | 超级肉类肉本质有限公司 | Mixed food containing cultured meat |
| CN112189050A (en) * | 2018-05-03 | 2021-01-05 | 莫萨肉类有限公司 | Device and method for producing tissue from cells |
| CN114554866A (en) * | 2019-09-10 | 2022-05-27 | 必要肉制品公司 | Avian stem cells for the production of food products |
-
2004
- 2004-09-17 CN CNA2004800440171A patent/CN101132803A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103747693A (en) * | 2011-07-26 | 2014-04-23 | 密苏里大学董事会 | Engineered comestible meat |
| CN103747693B (en) * | 2011-07-26 | 2017-08-01 | 密苏里大学董事会 | Edible engineering meat |
| CN106282093A (en) * | 2016-10-08 | 2017-01-04 | 上海生乐康生物技术发展有限公司 | A kind of production method of edible cell |
| CN110730615A (en) * | 2017-04-09 | 2020-01-24 | 超级肉类肉本质有限公司 | Mixed food containing cultured meat |
| US11589598B2 (en) | 2017-04-09 | 2023-02-28 | Supermeat The Essence Of Meat Ltd. | Cultured meat-containing hybrid food |
| CN112189050A (en) * | 2018-05-03 | 2021-01-05 | 莫萨肉类有限公司 | Device and method for producing tissue from cells |
| CN112189050B (en) * | 2018-05-03 | 2024-02-13 | 莫萨肉类有限公司 | Apparatus and method for producing tissue from cells |
| CN114554866A (en) * | 2019-09-10 | 2022-05-27 | 必要肉制品公司 | Avian stem cells for the production of food products |
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