Summary of the invention
The purpose of this invention is to provide that a kind of mattress surface magnetic field intensity is confirmed and anti-oxidant index detects after a period of time, blood routine detects, biochemical indicator detects the magnetic bed mattress that all obviously improves at the magnetic bed mattress that uses this magnetic field intensity.
Realize magnetic bed mattress of the present invention, the Surface field intensity of mattress is 1550~1650 Gausses.Using Surface field intensity is that 1550~1650 Gausses' magnetic bed mattress is after 2 months; With use before compare; Use back serum superoxide dismutases rising 9.1 ± 30.0U/mL (P>0.05); Whole blood glutathione peroxidase rising 9.2 ± 4.5U/mL (P>0.05), the serum MDA reduces 1.321.57nmol/mL (P<0.01); Serum levels of triglyceride reduces by 0.18 ± 4.21mmol/L (P<0.05), and serum lipoprotein (a) reduces by 3.59 ± 14.14mg/L (P<0.01); The serum homocysteine reduces by 3.49 ± 5.35 μ mol/mL (P=0.096).Simultaneously, significant difference does not all appear in experimenter's each item safety indexes (each item biochemical indicator that comprises routine blood test index and reaction liver, renal function) and each item detection index relevant with glycometabolism before and after the test.Explain that bedding can reduce the level of body oxidative damage and the heart, cranial vascular disease hazards in 2 months, and safety indexes and glycometabolism index of correlation are not had obviously by trial product to influence.
As improvement, the Surface field intensity of mattress is 1600 Gausses.Use through contrast to show, find that surperficial magnetic field intensity is that 1600 Gausses' magnetic bed mattress is best to human body body action effect.
As further improvement; Evenly distributed in magnetic bed mattress have a soft bar magnet; Soft bar magnet is the cohesive composite permanent magnetic material that contains neodymium iron nitrogen, wherein contains percentage by weight and be the neodymium iron nitrogen permanent-magnet powder of 85~97% neodymium iron nitrogen system, 2~12% binding agent and 1~3% compounding ingredient; Described binding agent is rubber or haloflex, and compounding ingredient comprises coupling agent, reinforcing filler, age resistor and plasticising lubricant; Neodymium iron nitrogen is that permanent-magnet powder is for having molecular formula NdFe
12~
xM
xN
yThe permanent-magnet powder of series, wherein M is Ti, Mo or W, X=1~2, Y=3~7.Use the soft bar magnet of above-mentioned material, the hot strength of soft bar magnet, non-oxidizability, pliability, pliability are good.
As improvement, in magnetic bed mattress, be provided with Temalen magnetic fibre layer, the Temalen magnetic fibre component of Temalen magnetic fibre layer is made up of polymer, magnetic particle, Temalen superfine powder, coupling agent; Temalen superfine powder: magnetic particle: coupling agent: polymer is 6~10: 10~14: 0.12~0.3: 380; The composition of Temalen superfine powder is any one in iron Tourmaline, lithium Tourmaline, the magnesium Tourmaline, and through after the ultra-fine grinding, average grain diameter is 0.2~0.5 μ m; The magnetic particle composition is one or more in tri-iron tetroxide, strontium ferrite, barium ferrite, Conjugate ferrite and neodymium iron boron, the tweezer iron boron, is 0.2~0.5 μ m through superfine modified average grain diameter; Coupling agent is aluminium titanium complexing agent, γ-glycidoxypropyltrimewasxysilane, γ-methacryloxy trimethoxy silane, N-β-(amino-ethyl)-γ TSL 8330, a kind of in the γ aminopropyltriethoxywerene werene.Temalen magnetic fibre of the present invention does not have injury to human body, can strengthen neural regulating action, promotes the blood circulation of whole body; Can effectively improve the oxygen content of cell, strengthen cell viability, improve PFT; Myocardial function improves sleep, enhances metabolism.
The wavelength of the Temalen magnetic fibre of Temalen magnetic fibre layer is the 4-14 micron, and wavelength is that 4-14 micron far infrared improves the blood of human body circulation, enhances metabolism, and emissivity surpasses 90%; Temalen functional fibre that can transmitting anion, make sleep environment be full of the fresh air as the forest: the Temalen material can bring dry and comfortable, warm, comfortable impression.
As the common improvement of such scheme, the evenly distributed soft bar magnet that 135 milli teslas are arranged in magnetic bed mattress, the centre distance between bar magnet and the bar magnet is 50~120mm, and bar magnet is arranged along the length direction of mattress, and bar magnet is arranged in the zone beyond the human body head.Head zone at human body is not arranged bar magnet, when using the magnetic pillow, can not cause magnetic disturbance, influences result of use, or causes harmful effect for people's body.Centre distance between bar magnet and the bar magnet is 50~120mm, and the size that can control bar magnet makes its requirement of satisfying comfort level, and it is too close that bar magnet is arranged.
As a kind of improvement; Magnetic bed mattress comprises picture-weaving in silk layer of cloth, Temalen magnetic fibre layer, nylon layer of cloth, non-woven cloth layer, wool fiber layer, cotton environment-protection fiber layer, multipole permanet magnet layer, Japanese import bridge formation polyethylene foamed layer, wear-resisting type picture-weaving in silk layer of cloth successively, and permanent soft bar magnet is evenly distributed between Temalen magnetic fibre layer and the nylon layer of cloth; Centre distance between bar magnet and the bar magnet is 93mm.Cotton environment-protection fiber of high-tech G.T and wool fiber layer have insulation and the thin airway dysfunction of moisture absorption; The bridge formation polyethylene foamed material of the protrudent sphere design of Japan's import is the main position of support human body and Shiatsu massage effect is provided fifty-fifty.
As another improvement; Magnetic bed mattress comprises non-woven cloth layer, Temalen magnetic fibre layer, waveform layer of polyurethane, hemispherical coconut palm palm fibre layer, layer of polyurethane, the wear-resisting type picture-weaving in silk layer of cloth of layer of cloth, the soft bar magnet of fixing protection successively, and permanent soft bar magnet is evenly distributed between non-woven cloth layer and the Temalen magnetic fibre layer; Centre distance between bar magnet and the bar magnet is 66mm.The polyurethane GT layer that hardness is moderate, the support of mattress, ventilative hydrofuge, mildew-resistant moisture-resistant are formed with the coconut palm palm fibre in the main position of support human body fifty-fifty; The waveform polyurethane material has thin gas, massage functions; The hemispherical coconut palm palm fibre layer of tool massage effect can reach effect cool in summer and warm in winter.
Improve as another kind; Picture-weaving in silk layer of cloth, the evenly fixedly layer of cloth of bar magnet, wool fiber layer, Temalen magnetic fibre layer, permanent soft bar magnet layer, wear-resisting type picture-weaving in silk layer; Permanent soft bar magnet is evenly distributed between Temalen magnetic fibre layer and the wear-resisting type picture-weaving in silk layer, and the centre distance between bar magnet and the bar magnet is 56mm.Airway dysfunction is dredged in insulation of wool fiber layer tool and moisture absorption.
Description of drawings
Fig. 1 is the structural representation of the embodiment of the invention 1;
Fig. 2 is the structural representation of the embodiment of the invention 2;
Fig. 3 is the structural representation of the embodiment of the invention 3;
Fig. 4 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse blood phase (WBC);
Fig. 5 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse blood phase (HGB);
Fig. 6 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse blood phase (PLT);
Fig. 7 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mouse body weight;
Fig. 8 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mouse spleen index;
Fig. 9 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mouse thymus index;
Figure 10 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse lymphocyte subpopulation (CD3);
Figure 11 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse lymphocyte subpopulation (CD19):
Figure 12 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse lymphocyte subpopulation (CD4/CD8);
Figure 13 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to bone marrow cells in mice PI;
Figure 14 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse spleen cell PI;
Figure 15 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse chest cell P1;
Figure 16 is that the present invention's 1600 Gauss's magnetic bed mattress are to the biochemical influence of mice serum (K);
Figure 17 is that the present invention's 1600 Gauss's magnetic bed mattress are to the biochemical influence of mice serum (Na);
Figure 18 is that the present invention's 1600 Gauss's magnetic bed mattress are to the biochemical influence of mice serum (Cl);
Figure 19 is that the present invention's 1600 Gauss's magnetic bed mattress are to the biochemical influence of mice serum (Mg);
Figure 20 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse blood sugar;
Figure 21 is that the present invention's 1600 Gauss's magnetic bed mattress are to the biochemical influence of mouse urea ammonia;
Figure 22 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mice serum liver flesh;
Figure 23 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mouse glutamic-pyruvic transaminase;
Figure 24 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mouse glutamic-oxalacetic transaminease;
Figure 25 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mice serum total protein;
Figure 26 is that the present invention's 1600 Gauss's magnetic bed mattress are to the albuminous influence of mice serum;
Figure 27 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mice serum globulin:
Figure 28 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mice serum alkaline phosphatase;
Figure 29 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mouse lactate dehydrogenase isoenzyme;
Figure 30 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to the mouse lactic dehydrogenase;
Figure 31 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse 'alpha '-hydroxy acids dehydrogenase:
Figure 32 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse creatine creatase isodynamic enzyme;
Figure 33 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse creatine creatase:
Figure 34 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse brain neurotransmitter (Asp ug/ml);
Figure 35 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse brain neurotransmitter (Glu ug/ml);
Figure 36 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse brain neurotransmitter (Gly ug/ml);
Figure 37 is the influences of the present invention's 1600 Gauss's magnetic bed mattress to mouse brain neurotransmitter (Glu ug/ml);
Figure 38 is normal mouse frame ball pathological section figure (HEx400);
Figure 39 is according to back two months mouse testis pathological section figure (HEx400);
Figure 40 is according to back four months mouse testis pathological section figure (HEx400);
Figure 41 is according to back one month mouse testis pathological section figure (HEx400);
Figure 42 is according to back three months mouse testis pathological section figure (HEx400);
Figure 43 is according to back five months mouse testis pathological section figure (HEx400);
Figure 44 is according to back six months mouse testis pathological section figure (HEx400);
Figure 45 is according to back seven months mouse testis pathological section figure (HEx400);
Figure 46 is according to back eight months mouse testis pathological section figure (HEx400).
The specific embodiment
Embodiment 1
As shown in Figure 1; A kind of magnetic bed mattress; Comprise picture-weaving in silk layer of cloth 1, Temalen magnetic fibre layer 2, nylon layer of cloth 3, non-woven cloth layer 4, wool fiber layer 5, cotton environment-protection fiber layer 6, multipole permanet magnet layer 7, Japanese import bridge formation polyethylene foamed layer 8, wear-resisting type picture-weaving in silk layer of cloth 9 successively, permanent soft bar magnet 10 is evenly distributed between Temalen magnetic fibre layer 2 and the nylon layer of cloth 3.Permanent soft bar magnet is the soft bar magnet of 135 milli teslas, and the centre distance between bar magnet and the bar magnet is 96mm, and the Surface field intensity of mattress is 1500 Gausses.
Soft bar magnet 10 is 91% neodymium iron nitrogen titanium permanent-magnet powder for containing the cohesive composite permanent magnetic material of neodymium iron nitrogen, wherein containing percentage by weight, and 7% rubber and 2% compounding ingredient, compounding ingredient comprise coupling agent, reinforcing filler, age resistor and plasticising lubricant.The Temalen magnetic fibre component of Temalen magnetic fibre layer 2 is made up of polymer, magnetic particle, Temalen superfine powder, coupling agent; Temalen superfine powder: magnetic particle: coupling agent: polymer is 8: 12: 0.21: 380; The composition of Temalen superfine powder is the iron Tourmaline, and through after the ultra-fine grinding, average grain diameter is 0.35 μ m: the magnetic particle composition is a tri-iron tetroxide, is 0.35 μ m through superfine modified average grain diameter; Coupling agent is an aluminium titanium complexing agent.The wavelength of the Temalen magnetic fibre of agate grain magnetic fibre layer is 9 microns.
Embodiment 2
As another improvement; Magnetic bed mattress comprises non-woven cloth layer 12, Temalen magnetic fibre layer 13, waveform layer of polyurethane 14, hemispherical coconut palm palm fibre layer 15, layer of polyurethane 16, the wear-resisting type picture-weaving in silk layer of cloth 17 of layer of cloth 11, the soft bar magnet of fixing protection successively, and permanent soft bar magnet 18 is evenly distributed between non-woven cloth layer 12 and the Temalen magnetic fibre layer 13.Permanent soft bar magnet is the soft bar magnet of 135 milli teslas, and the centre distance between bar magnet and the bar magnet is 66mm, and the Surface field intensity of mattress is 1600 Gausses.
Soft bar magnet 18 is that 85% neodymium iron nitrogen tungsten is permanent-magnet powder for containing the cohesive composite permanent magnetic material of neodymium iron nitrogen, wherein containing percentage by weight, and 12% rubber and 3% compounding ingredient, compounding ingredient comprise coupling agent, reinforcing filler, age resistor and plasticising lubricant.
The Temalen magnetic fibre component of Temalen magnetic fibre layer 13 is made up of polymer, magnetic particle, Temalen superfine powder, coupling agent; Temalen superfine powder: magnetic particle: coupling agent: polymer is 6: 10: 0.12: 380; The composition of Temalen superfine powder is the lithium Tourmaline, and through after the ultra-fine grinding, average grain diameter is 0.5 μ m; The magnetic particle composition is the mixture of strontium ferrite, Conjugate ferrite and neodymium iron boron, is 0.5 μ m through superfine modified average grain diameter; Coupling agent is a kind of of γ-glycidoxypropyltrimewasxysilane.The wavelength of the Temalen magnetic fibre of agate grain magnetic fibre layer is 14 microns.
Embodiment 3
A kind of magnetic bed mattress; Comprise picture-weaving in silk layer of cloth 20, the evenly fixedly layer of cloth 21 of bar magnet, wool fiber layer 22, Temalen magnetic fibre layer 23, permanent soft bar magnet layer 24, wear-resisting type picture-weaving in silk layer 25 successively, the permanent soft bar magnet of permanent soft bar magnet layer 24 is evenly distributed between Temalen magnetic fibre layer 23 and the wear-resisting type picture-weaving in silk layer 25.Permanent soft bar magnet is the soft bar magnet of 135 milli teslas, and the centre distance between bar magnet and the bar magnet is 56mm, and the Surface field intensity of mattress is 1650 Gausses.
Soft bar magnet is the cohesive composite permanent magnetic material that contains neodymium iron nitrogen; Wherein contain percentage by weight and be the neodymium iron nitrogen permanent-magnet powder of 97% neodymium iron nitrogen tungsten system; 2% haloflex and 1% compounding ingredient, compounding ingredient comprise coupling agent, reinforcing filler, age resistor and plasticising lubricant.
The Temalen magnetic fibre component of Temalen magnetic fibre layer 23 is made up of polymer, magnetic particle, Temalen superfine powder, coupling agent; Temalen superfine powder: magnetic particle: coupling agent: polymer is 10: 14: 3: 380; The composition of Temalen superfine powder is any one in the magnesium Tourmaline, and through after the ultra-fine grinding, average grain diameter is 0.2 μ m; The magnetic particle composition is the mixture in barium ferrite, Conjugate ferrite and neodymium iron boron, the tweezer iron boron, is 0.2 μ m through superfine modified average grain diameter; Coupling agent is the γ aminopropyltriethoxywerene werene.The wavelength of the Temalen magnetic fibre of agate grain magnetic fibre layer is 4 microns.
Beneficial effect
One, Temalen health care magnetic bed mattress effect property evaluation
1 materials and methods;
1.1 receive trial product: Surface field is 1600 Gauss's Temalen magnetic fibre health care bed mattress.
1.2 the experimenter includes standard in: press the principle of voluntariness and select 45-77 year, physical condition is better, does not have obvious brain, the heart, liver, lung, kidney, blood illness, does not have the history of taking medicine for a long time, the crowd that aspiration is tried.
1.3 eliminator's standard:
1.3.1 the artificial or electronic heart pacemaker person is installed in the body
1.3.2 hemorrhagic disease person is arranged
1.3.3 be the operator with interior in three months
1.3.4 metallics (like joint prosthesis) person has been installed in the body
1.3.5 the hyperfunction patient of thyroid gland
1.3.6 serious heart, kidney, liver diseases patient
1.3.7 carry out coronary bypass, placing rack person
1.3.8 severe arteriosclerotic
1.3.9 malignant tumor patient
1.3.10 high fever patient
1.3.11 active tuberculosis patient
1.3.12 epileptic
1.3.13 glaucoma patient
1.4 method for using and time: require the experimenter when sleep (comprising afternoon nap and nighttime sleep), to use a complete set of product during being tried.Used altogether 2 months.Former life during being tried, eating habit are constant.
1.5. main detecting instrument: Hitachi's 7600 automatic clinical chemistry analyzers, MD continuous spectrum plate reading, the full-automatic blood counting instrument of Japanese photoelectricity.
2.. observation index: each checks once each item index before and after test.
2.1 ordinary circumstance: understand experimenter's sleep, spirit, diet, stool and urine situation.
2.2 anti-oxidant index detects: serum mda content (MDA), serum superoxide dismutases (SOD), whole blood glutathione peroxidase (GSH-px).
2.3 blood routine detects: red blood cell count(RBC), blood red egg oneself, white blood cell count(WBC).
2.4 biochemical indicator detects:
Serum glutamic pyruvic transminase (GPT); Glutamic-oxalacetic transaminease (GOT); Urea nitrogen (BUN); Creatinine (CRE); Total protein (TP); Albumin (ALB) blood sugar (GLUC); Saccharification haemocyanin (GSP); C-peptide (C PEPTID); Insulin (INSULIN); Triglyceride (TG); T-CHOL (TC); HDL-C (HDL-C); LDL-C (LDL-C); APoA 1 (ApoA1); Apolipoprotein B (ApoB); Lipoprotein (a) (LP (a)); Homocysteine (HCY); C-reactive protein (CRP).
3. result of the test:
3.1 general situation:
Observe experimenter's 30 examples altogether, the male sex's 11 examples wherein, women's 19 examples, minimum 45 years old of age, maximum 77 years old, average 61.9 ± 9.5 years old.Mental status was better during the experimenter was tried, and diet, stool and urine are normal, 90% experimenter think use receive trial product after sleep quality improve, particularly original sleep state gastrointestinal disease patient thinks that sleep improves obviously.
3.2 anti-oxidant index testing result: (seeing table 1)
The influence of table 1 health one hundred healthy bedroom series of products antagonism oxidation index
Annotate: * *, with use before compare: P<0.01.
Visible by the listed result of table 1, before serum MDA concentration was starkly lower than use after using, through statistical test, difference had conspicuousness, explains and uses these series of products can reduce body oxidative damage level in 2 months.In addition, compare rising to some extent before using back activity of SOD in serum and whole blood GSH-px activity and using, but difference there is not conspicuousness yet.
3.3 routine blood test index testing result: (seeing table 2)
Table 2 health one hundred healthy bedroom series of products are to the influence of routine blood test index
Visible by the listed result of table 2, all experimenter's hemoglobins before and after the test, red blood cell count(RBC), from indexs such as cell count, leukocyte differential counts all in range of normal value, explain that these series of products of use 2 months do not have obvious influence to the routine blood test index.
3.4 biochemical indicator testing result:
3.4.1 liver, renal function index of correlation: (seeing table 3)
Table 3 health one hundred healthy bedroom series of products are to the influence of liver, renal function index of correlation
Visible by the listed result of table 3; Experimenter's total serum protein (TP), white egg be from (ALB), glutamic-pyruvic transaminase (GPT), glutamic-oxalacetic transaminease (GOT), urea nitrogen (BUN), the creatinine equal no significant differences of index such as (CRE) before and after using, and explain that these series of products of use 2 months do not have obvious influence to liver, renal function.
3.4.2 glycometabolism index of correlation: (seeing table 4)
Table 4 health one hundred healthy bedroom series of products are to the influence of glycometabolism index of correlation
Visible by the listed result of table 4; Experimenter's blood sugar (GLUC), saccharification haemocyanin (GSP), C-peptide (C PEPTID), insulin index no significant differences such as (INSULIN) before and after using explains that these series of products of use 2 months do not have obvious influence to the glycometabolism index of correlation.
3.4.3 fat metabolism index of correlation: (seeing table 5)
Visible by the listed result of table 5; Before using back serum TG, LP (a) to be starkly lower than use; Through statistical test, difference has conspicuousness (P<0.05 or P<0.01), explains and uses these series of products can reduce serum TG and LP two fat metabolism indexs of correlation such as (a) in 2 months.Significant change does not appear in other index.
Table 5 health one hundred healthy bedroom series of products are to the influence of fat metabolic index
Annotate: *, with use before compare: P<0.05; *, with use before compare: P<0.01.
3.4.4 homocysteine (HCY), C-reactive protein (CRP) testing result: (seeing table 6)
Table 6 health one hundred healthy bedroom series of products are to the influence of serum homocysteine and C-reactive protein
Visible by the listed result of table 6, before serum homocysteine is lower than use after using, through statistical test; Difference does not have conspicuousness (P=0.096); But, it should be noted that HCY after 77.7% experimenter uses is lower than use before; Simultaneously, HCY surpasses the HCY that 17 people are arranged among 18 experimenters of normal reference value (15.0 μ mol/L) and decline in various degree occurs; Significant change does not appear in CRP.
4 conclusions:
30 routine experimenters used health one hundred healthy bedroom series of products (the Temalen health care is at bedding, mattress) 2 months on request; With use before compare; Use back serum superoxide dismutases rising 9.1 ± 30.0U/mL (P>0.05); Whole blood glutathione peroxidase rising 9.2 ± 4.5U/mL (P>0.05), the serum MDA reduces by 1.32 ± 1.57nmol/mL (P<0.01); Serum levels of triglyceride reduces by 0.18 ± 1.21mmol/L (P<0.05), and serum lipoprotein (a) reduces by 3.59 ± 14.14mg/L (P<0.01); The serum homocysteine reduces by 3.49 ± 5.35 μ mol/mL (P=0.096).Simultaneously, significant difference does not all appear in experimenter's each item safety indexes (each item biochemical indicator that comprises routine blood test index and reaction liver, renal function) and each item detection index relevant with glycometabolism before and after the test.The level of using health one hundred healthy bedroom series of products (Temalen health care bedding, mattress) can reduce body oxidative damage and the heart, cranial vascular disease hazards in 2 months is described, and safety indexes and glycometabolism index of correlation are not had obviously by trial product to influence.
Two, Surface field is that 1600 Gausses' magnetic bed mattress influences body
For long-term use Magnetotherapeutic health product, whether can produce harmful effect to body, be a still unsolved major issue that perplexs manufacturer and consumer for a long time.Be the test whether magnetic bed mattress that uses 1600 Gausses is influenced body below.
Experiment purpose
With the BALB/C mouse that grows up is model, inquires into long-term (8 months) the 1600 Gauss's magnetic bed mattress that use whether the body related system is produced adverse effect, and the theoretical foundation of science is provided for design, technology and the scope of application of health one hundred magnetic bed mattress.
Materials and methods
Animal used as test and grouping
160 of adult BALB/C mouse (pure lines) (female, hero half and half), body weight 20 ± 2 grams are provided and unified raising 21~23 ℃ of indoor temperatures, relative humidity 60% by Military Medical Science Institute's Experimental Animal Center.Animal used as test is divided into 2 groups at random: normal control group (80 female, heros half and half), experimental group (80 female, heros half and half).
Illuminating method
The soft bar-shaped permanet magnet of 1600 Gausses (hereinafter to be referred as soft bar magnet) that adopts the healthy bedroom of Guangzhou health one hundred Co., Ltd to provide, magnetic mattress is used in the self-control experiment.It is 1600 Gauss's magnetic mattress irradiation 12 hours that the experimental group animal places magnetic field intensity every day.
Draw materials
Respectively at after the treatment with irradiation 1,2,3,4,5,6,7,8 month, the tissues such as peripheral blood, brain tissue, marrow, thymus gland, spleen, testis of getting normal group (10 female, male half and half) and treatment with irradiation group (10 female, male half and half) experimental group animal carried out coherent detection.
Observation index
1. ordinary circumstance: existing outward, movable, ingest, the drainage situation.
2. blood routine detects: white blood cell count(WBC), blood red egg counting, platelet count.
3. body weight and organ index: body weight, index and spleen index, thymus index.
4. lymphocyte subpopulation: CD3, CD19, CD4/CD8.
5. marrow, spleen and thymocyte proliferation index: bone marrow cell PI, spleen cell PI, thymocyte PI.
6 serum biochemistries detect: serum K, serum N a, change of serum C l, serum Mg, blood sugar, urea nitrogen, serum flesh liver, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease, total serum protein, seralbumin, serum globulins, serum alkaline phosphatase, lactate dehydrogenase isoenzyme, lactic dehydrogenase, AHB, creatine creatase isodynamic enzyme, creatine creatase.
7. neurotransmitter detects: aspartic acid, glutamic acid, glycine, GABA.
8. testis pathological section
Main detecting instrument
The full-automatic blood counting instrument of Japanese photoelectricity, U.S. company BD FACSC flow cytometer, Hitachi's 7600 automatic clinical chemistry analyzers, Hewlett-Packard's high performance liquid chromatograph, Hp1050 pump, Hp1046A fluorescence detector etc.
Experimental result
Ordinary circumstance: between the animal used as test survival period outer existing, movable, ingest, all no abnormal situation such as drainage.
Blood routine testing result: (seeing table 7)
Table 7 1600Gs stationary magnetic field is to the influence
of mouse blood phase
By table 7 and visible like the listed result of Fig. 4-6, through statistical procedures, the conventional value of each irradiation group peripheral blood is compared there was no significant difference with control group.1600 gauss magnetic field long term bodies are described, PBL, blood red egg, blood platelet are not had obvious influence.
Body weight and organ index testing result: (seeing table 8)
Table 8 1600Gs stationary magnetic field is to the influence
of mouse body weight and organ index
Visible by table 8 and the listed result of Fig. 7-9, the animal used as test body weight increases with the prolongation of life span.Organ index is one of important indicator of this organ dysfunction of reflection, and through statistical procedures, each irradiation group index and spleen index value is compared there was no significant difference with control group.Though thymus index is on a declining curve with the prolongation of life span, compare there was no significant difference with control group, belong to normal degenerative change.Explain that 1600 gauss magnetic field long term bodies do not have obvious influence to thymus gland, spleen.
Lymphocyte subpopulation testing result: (seeing table 9)
Table 9 1600Gs stationary magnetic field is to the influence
of mouse lymphocyte subpopulation
With the ratio of Flow cytometry CD3, CD19 and CD4/CD8, be the present popular accurate judgement cellular immunity and the method for humoral immune function.Visible by table 9 and the listed result of Figure 10-12, through statistical procedures, the ratio of each irradiation group CD3, CD19 and CD4/CD8 is compared there was no significant difference with control group.1600 gauss magnetic field long terms are described, body's immunity is not had obvious influence.
Marrow, spleen and thymocyte proliferation index testing result; (seeing table 10)
Table 1600Gs stationary magnetic field is to the influence
of mouse bone marrow cells, spleen and thymocyte proliferation index (PI)
With Flow cytometry marrow, spleen and thymocyte proliferation index (PI), be the method for hematopoiesis of a kind of accurate judgement body and immunologic function.Visible by table 10 and the listed result of Figure 13-15, through statistical procedures, each irradiation group marrow, spleen and thymocyte proliferation exponential quantity are compared there was no significant difference with control group.1600 gauss magnetic field long terms are described, body hematopoiesis and immunologic function are not had obvious influence.
1 serum biochemistry test result: (seeing table 11)
Table 11 1600Gs stationary magnetic field is to the biochemical influence
of mice serum
Continuous table 11 1600GS stationary magnetic field is to the biochemical influence
of mice serum
Continuous table 11 1600Gs stationary magnetic field is to the biochemical influence
of mice serum
Continuous table 11 1600Gs stationary magnetic field is to the biochemical influence
of mice serum
Continuous table 11 1600GS stationary magnetic field is to the biochemical influence
of mice serum
Continuous table 11 1600Gs stationary magnetic field is to the biochemical influence
of mice serum
Above-mentioned biochemical indicator reflected the 1600Gs stationary magnetic field to liver, kidney, myocardial function with to and the influence of aspects such as glycometabolism, fat metabolism.By table 11 and continuous table thereof with the listed result of Figure 16-33 it is thus clear that, through statistical procedures, irradiation group each item biochemical indicator numerical value is compared there was no significant difference with control group.1600 gauss magnetic field long terms are described, to body liver, kidney, myocardial function with to and aspects such as glycometabolism, fat metabolism do not have obvious influence.
The neurotransmitter testing result:
According to back 1 month (μ g/ml)
According to back 2 months (μ g/ml)
According to back 3 months (μ g/ml)
According to back 4 months (μ g/ml)
According to back 5 months (μ g/ml)
According to back 6 months (μ g/ml)
According to back 7 months (μ g/ml)
According to back 8 months (μ g/ml)
We have detected the influence of 1600 Gauss stationary magnetic fields to excitability and inhibiting nerve mediator (aspartic acid Asp, glutamic acid Glu, glycine Gly and γ one reanal GABA) with high pressure lipuid chromatography (HPLC) (HPLC), and the result adopts in groups data to get the t check.Through statistical procedures, irradiation group each item numerical value is compared otherness with control group not remarkable.1600 gauss magnetic field long terms are described, the function aspects of body central nervous system is not had obvious influence.
The testis pathological section
Like Figure 38 to 46 diagram, visible by the testis pathological section: each irradiation group testes histological structure is normal basically, seminiferous tubule queueing discipline, tight.Each phase spermatid is not seen obvious abnormal change on form, most of androgone gets quantity and also do not see remarkable change.Spermatoblast degeneration necrosis in few part tubule.Testis phase matter is not seen remarkable change.
Conclusion
Carrying out for a long time to 160 animals, the observed result of (8 months), system proves: animal used as test is accepted 1600 gauss magnetic field effects for a long time, and hematopoiesis, immunity, reproduction, the central nervous system of body do not produced adverse effect.
The above results is also pointed out us: use 1600 Gauss's magnetic bed mattress also can not produce adverse effect to hematopoiesis, immunity, reproduction, the nervous system of human body for a long time.
Do experiment with 1550 gaussian sums, 1650 Gauss's magnetic bed mattress,, also can not produce harmful effect function of human body to the health care of human body magnetic bed mattress near 1600 Gausses.