CN101100643A - Gloeostereum incarnatum plant culture liquid bacterial culturing method - Google Patents
Gloeostereum incarnatum plant culture liquid bacterial culturing method Download PDFInfo
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- CN101100643A CN101100643A CNA2006100896344A CN200610089634A CN101100643A CN 101100643 A CN101100643 A CN 101100643A CN A2006100896344 A CNA2006100896344 A CN A2006100896344A CN 200610089634 A CN200610089634 A CN 200610089634A CN 101100643 A CN101100643 A CN 101100643A
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Abstract
A method for culturing liquid bacterium from carneus glue ligament bacterium is carried out by transferring elm slant into first-level liquid seed shake flask, fermenting, culturing, transferring first-level liquid seed into second-level liquid seed shake flask according to 5-20% seed volume, fermenting, culturing, transferring second-level liquid seed into fermenting device, culturing to obtain seed liquid bacterium, transferring it into solid bag and artificial culturing.
Description
Technical field
The invention belongs to field of edible fungus culture, particularly relate to Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) cultivation strain cultivation method.
Background technology
Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) [Gloeostereum incarnatum], another name elm ear, elm mill.Belong to Basidiomycotina, Hymenomycetes, Aphyllophorales, photovoltaicing leather bacteria section, gleoesterum incarnatum genus.Wild Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) mainly is distributed in China the Northeast and Hokkaido, Japan, the Tieling in Liaoning Province, former clearly, new guest, Benxi and Fushun, the Tonghua in Jilin Province, Huijiang, Fusong, long white and Antu etc., the Eastern Mountain Area of Heilongjiang Province is the main producing region of Gloeostereum incarnatum S.Ito etI mai (G.L C F8511), and also there is a small amount of distribution in Xinjiang.
Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) is famous dietotherapeutic fungi.It introduces the existing long history of Chinese medicine.The famous medical scholar's LI Shi-Zhen of the Ming Dynasty is shown in the Compendium of Material Medica and to be carried: " the elm ear is adopted it August ", " August, the elm ear exposed to the sun with the good wine stain, and same foxtail millet seed, real the cooking of purple amaranth are the end.Every clothes a dose of powder just as much as the amount can be taken up with three fingers, under the wine, it is not hungry to make us warding off paddy." Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) sporophore nutritive ingredient is very abundant.Analyze according to Chinese Academy Of Preventive Medicine Research Institute Of Nutrition And Food Hygiene, Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) mainly contains protein, carbohydrate, vitamin-E, vitamins B
1, vitamins B
2, necessary each seed amino acid of human body such as trace element such as calcium, phosphorus, iron, zinc and L-glutamic acid, Methionin.
The Jilin Tang Yu of special product higher junior college qin, Zhao Yitao have delivered " elm ear high-yield culture technique " article on " agricultural technology service " magazine of 2003 the 7th phases, this piece article discloses some Pleurotus ulmarius (Bullex Franch) Quel fungus artificial cultivation techniques.
At present, mainly based on wild and artificial culture, tame bacterial classification still adopts the solid culture mode in the production of China's Gloeostereum incarnatum S.Ito etI mai (G.L C F8511), and the production cycle is longer.
Summary of the invention:
The subject matter that invention solves provides Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) batch production production or artificial culture strain cultivation technology.
The present invention adopts the strain cultivation technology generation to replace the solid spawn preparation method who adopts in the edible fungus culturings such as traditional elm ear, technological overview is as follows: elm ear inclined-plane bacterial strain is transferred to the level liquid seed and shakes fermentation culture in the bottle, then the level liquid seed is transferred to the secondary liquid seeds according to the inoculum size of 5-20% again and shakes fermentation culture in the bottle, the secondary liquid seeds is transferred to fermentor cultivation, obtains inoculation and use liquid spawn; Liquid spawn inserted carry out artificial culture in the solid Bag Material.
Particular content comprises:
(1) first order seed is cultivated: access of gleoesterum incarnatum slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of substratum and cultivate culture condition: 80～180 rev/mins of rotary shaking tables, cultivated about 48～80 hours for 22～28 ℃;
(2) secondary seed is cultivated: the one-level shake-flask seed is inserted 3 liters that 1 liter of substratum is housed with 5～20% inoculum sizes shake in the bottle and cultivate culture condition: 80～180 rev/mins of rotary shaking tables, cultivated about 48～72 hours for 22～28 ℃;
(3) seed tank culture: it is 150 liters seeding tank that the inoculum size with 5～20% inserts the cubic capacity that 100 liters of fermention mediums are housed to secondary seed.Culture condition is: 22～28 ℃ of culture temperature, and 80～180 rev/mins of stirring velocitys, ventilation (V/V) 1: 0.3～0.8, tank pressure 0.03～0.1Mpa cultivated 36～56 hours;
Fermentor cultivation finishes liquid spawn inserted in the sacked material after the sterilization according to the inoculum size of 1-10% by aseptic flexible pipe and adopts the factory culture mode to cultivate.
Adopting technology of the present invention to cultivate bacterial classification can be fast and the mass-producing cultivation, compares with the conventional solid vaccination ways, has shortened the spawn culture time greatly, has greatly reduced expending of personnel, has reduced the production cost of factory; Since adopt same cell age for the strong bacterial classification of logarithmic phase, thereby guaranteed to send out in the bacterium operation quality homogeneous and be difficult for bacteria infection in the later stage, the incubation time of sporophore and solid vaccination mode also shorten dramatically.
The gleoesterum incarnatum bacterial classification is purchased in Chinese agriculture microorganism center among the present invention, bacterium number: ACCC50314, ACCC50363, ACCC50365, ACCC50394, ACCC50395, ACCC50414, ACCC50423, bacterial classifications such as ACCC50471.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
Seed culture medium consists of among the present invention: starch 1%, sucrose 3%, glucose 2%, peptone 0.5%, defatted soybean protein powder 0.1%, potassium primary phosphate 0.2%, sal epsom 0.1%, vitamins B
12PPm, PH6.0.
Fermention medium consists of: starch 3%, sucrose 3%, glucose 3%, peptone 0.5%, defatted soybean protein powder 1.5%, potassium primary phosphate 0.2%, sal epsom 0.1%, vitamins B
12PPm, PH6.0.
Embodiment
Embodiment 1
(1) first order seed is cultivated: access of gleoesterum incarnatum slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of substratum and cultivate culture condition: 80 rev/mins of rotary shaking tables, cultivated about 48 hours for 25 ℃;
(2) secondary seed is cultivated: the one-level shake-flask seed is inserted 3 liters that 1 liter of substratum is housed with 10% inoculum size shake in the bottle and cultivate culture condition: 100 rev/mins of rotary shaking tables, cultivated about 48 hours for 25 ℃;
(3) seed tank culture: it is 150 liters seeding tank that the inoculum size with 5% inserts the cubic capacity that 100 liters of fermention mediums are housed to secondary seed.Culture condition is: 25 ℃ of culture temperature, and 120 rev/mins of stirring velocitys, ventilation (V/V) 1: 0.4, tank pressure 0.05Mpa cultivated 48 hours;
Fermentor cultivation finishes liquid spawn inserted in the sacked material after the sterilization according to 2% inoculum size by aseptic flexible pipe and adopts the factory culture mode to cultivate.
The gleoesterum incarnatum bacterial classification is purchased in Chinese agriculture microorganism center among the present invention, bacterium number: ACCC50314.
Seed culture medium consists of among the present invention: starch 1%, sucrose 3%, glucose 2%, peptone 0.5%, defatted soybean protein powder 0.1%, potassium primary phosphate 0.2%, sal epsom 0.1%, vitamins B
12PPm, PH6.0.
Fermention medium consists of: starch 3%, sucrose 3%, glucose 3%, peptone 0.5%, defatted soybean protein powder 1.5%, potassium primary phosphate 0.2%, sal epsom 0.1%, vitamins B
12PPm, PH6.0.
Embodiment 2
(1) first order seed is cultivated: access of gleoesterum incarnatum slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of substratum and cultivate culture condition: 120 rev/mins of rotary shaking tables, cultivated about 70 hours for 23 ℃;
(2) secondary seed is cultivated: the one-level shake-flask seed is inserted 3 liters that 1 liter of substratum is housed with 10% inoculum size shake in the bottle and cultivate culture condition: 140 rev/mins of rotary shaking tables, cultivated about 72 hours for 23 ℃;
(3) seed tank culture: it is 150 liters seeding tank that the inoculum size with 10% inserts the cubic capacity that 100 liters of fermention mediums are housed to secondary seed.Culture condition is: 24 ℃ of culture temperature, and 130 rev/mins of stirring velocitys, ventilation (V/V) 1: 0.8, tank pressure 0.05Mpa cultivated 48 hours;
Fermentor cultivation finishes liquid spawn inserted in the sacked material after the sterilization according to 4% inoculum size by aseptic flexible pipe and adopts the factory culture mode to cultivate.
The gleoesterum incarnatum bacterial classification is purchased in Chinese agriculture microorganism center, bacterium ACCC50363 among the present invention
Seed culture medium consists of among the present invention: starch 1%, sucrose 3%, glucose 2%, peptone 0.5%, defatted soybean protein powder 0.1%, potassium primary phosphate 0.2%, sal epsom 0.1%, vitamins B
12PPm, PH6.0.
Fermention medium consists of: starch 3%, sucrose 3%, glucose 3%, peptone 0.5%, defatted soybean protein powder 1.5%, potassium primary phosphate 0.2%, sal epsom 0.1%, vitamins B
12PPm, PH6.0.
Claims (3)
1. Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) factory culture strain cultivation method, it is characterized in that comprising the steps: that Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) inclined-plane bacterial strain is transferred to the level liquid seed and shakes fermentation culture in the bottle, then the level liquid seed is transferred to the secondary liquid seeds and shakes fermentation culture in the bottle, the secondary liquid seeds is transferred to fermentor cultivation, obtains inoculation and use liquid spawn.
2. according to the described a kind of Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) factory culture strain cultivation method of claim 1, wherein said cultural method is as follows: (1) first order seed is cultivated: access of gleoesterum incarnatum slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of substratum and cultivate, culture condition: 80～180 rev/mins of rotary shaking tables, cultivated about 48～80 hours for 22～28 ℃; (2) secondary seed is cultivated: the one-level shake-flask seed is inserted 3 liters that 1 liter of substratum is housed with 5～20% inoculum sizes shake in the bottle and cultivate culture condition: 80～180 rev/mins of rotary shaking tables, cultivated about 48～72 hours for 22～28 ℃; (3) seed tank culture: it is 150 liters seeding tank that the inoculum size with 5～20% inserts the cubic capacity that 100 liters of fermention mediums are housed to secondary seed.Culture condition is: 22～28 ℃ of culture temperature, and 80～180 rev/mins of stirring velocitys, ventilation (V/V) 1: 0.3～0.8, tank pressure 0.03～0.1Mpa cultivated 36～56 hours.
3. according to the described a kind of Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) factory culture strain cultivation method of claim 1, wherein said Gloeostereum incarnatum S.Ito etI mai (G.L C F8511) is ACCC50314, ACCC50363, ACCC50365, ACCC50394, ACCC50395, ACCC50414, ACCC50423 or ACCC50471.
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| CNA2006100896344A CN101100643A (en) | 2006-07-07 | 2006-07-07 | Gloeostereum incarnatum plant culture liquid bacterial culturing method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081723A (en) * | 2013-02-17 | 2013-05-08 | 哈尔滨伟平科技开发有限公司 | Preparation method for Gloeostereum incarnatum powder |
| CN103141300A (en) * | 2013-02-21 | 2013-06-12 | 北京绿科天成生物科技有限公司 | Stereum hirsutum fermentation liquor, sesquiterpene and applications thereof |
-
2006
- 2006-07-07 CN CNA2006100896344A patent/CN101100643A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081723A (en) * | 2013-02-17 | 2013-05-08 | 哈尔滨伟平科技开发有限公司 | Preparation method for Gloeostereum incarnatum powder |
| CN103141300A (en) * | 2013-02-21 | 2013-06-12 | 北京绿科天成生物科技有限公司 | Stereum hirsutum fermentation liquor, sesquiterpene and applications thereof |
| CN103141300B (en) * | 2013-02-21 | 2015-12-02 | 北京绿科天成生物科技有限公司 | Stereum hirsutum fermentation and sequiterpene and application thereof |
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