CN101095814A - Liposoluble effective extracts from onion white and the extracting method thereof - Google Patents
Liposoluble effective extracts from onion white and the extracting method thereof Download PDFInfo
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- CN101095814A CN101095814A CNA2006100195378A CN200610019537A CN101095814A CN 101095814 A CN101095814 A CN 101095814A CN A2006100195378 A CNA2006100195378 A CN A2006100195378A CN 200610019537 A CN200610019537 A CN 200610019537A CN 101095814 A CN101095814 A CN 101095814A
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Abstract
The invention discloses a scallion stalk fat-soluble effective extract and the extraction method. The method comprises following steps: disintegrating one or the combination of dry scallion stalk or dry onion juice or dry onion, cold soaking or warm extracting with one of ether, ligarine, chloroform, acetic acid ethyl ester, butanol and ethanol for 1-24 hours, filtering extraction liquid, condensing extraction liquid to remove solvent and getting fat soluble effective extract. The ratio between said raw material and solvent is 1g: 6-15ml, the relative density of said fat soluble effective extract at 20 Deg. C is 0.8000-0.9000; the refractive index at 20 Deg. C is 1.2500-1.4500.The main component in said fat soluble extract comprises volatile oil, steroidal saponins compound, chromocor compound, vitamin and polysaccharide. The extract effect for treating cardiovascular disease is distinctive. The extraction process is simple, controllable and stable.
Description
Technical field
The invention belongs to plant extract and extracting method, being specifically related to a kind of is the extract and the extracting method of the very light blue liposoluble substance of raw material with very light blue or dried Sucus Allii Fistulosi or full Herba Alii fistulosi, specifically adopts solvent method to extract.
Background technology
Very light blue title is according to the medicinal custom of history, and so the dispersing wind-cold exterior syndrome is all with very light blue and name.Its source is the fresh bulb of liliaceous plant shallot Allium fisturosum L.var.caespitosum Makio or chive Allium schoenoprasum.The four seasons can gather, and clean using fresh herb.Herba Alii fistulosi beginning alpine rush or palm-bark rain cape be stated from { Newly Revised Canon of Materia Medica } " its human world food Herba Alii fistulosi has 2 kinds again, and the Herba Alii fistulosi of freezing is arranged, and is promptly not dead through the winter, divide stem to plant to transplant and loss of fecundity also; Chinese Herba Alii fistulosi is arranged again, and the winter is that leaf is withered ", the former is like shallot, and the latter is like chive.The Compendium of Material Medica cloud: " shallot is kind Herba Alii fistulosi ..., stem is soft and fine and fragrant, can be through the winter, wooden Herba Alii fistulosi of Chinese Herba Alii fistulosi, its stem is thick and stiff, so the wood name is arranged.The shallot loss of fecundity, Chinese Herba Alii fistulosi spring end is bloomed into clump, Bluish white, its sub-acrid in the mouth, darkish complexion has wrinkle, makes 3 flaps. describe according to it, the person like shallot, the latter is like Herba Alii fistulosi.Very light bluely be distributed widely in the Northern Hemisphere, the centuries, very light blue in the Middle East and the Far East Area extensively cultivate, very light blue abundant in china natural resources, be mainly used in vegetable and flavoring agent.And do not have the report of the effective extract in the fresh bulb of open shallot Allium fisturosum L.var.caespitosum Makio of document or chive Allium schoenoprasum at present.
Summary of the invention
The object of the present invention is to provide liposoluble effective extracts and extracting method thereof in the fresh bulb of a kind of shallot Allium fisturosum L.var.caespitosum Makio or chive Allium schoenoprasum, adopt solvent method to extract.
The technical scheme that realizes one of the object of the invention is: lipophilic effective extract of Chinese green onion, it is after one or more the combination in exsiccant very light blue or dried Sucus Allii Fistulosi or the dry full Herba Alii fistulosi is pulverized, adopt a kind of solvent cold soak or heat in ether, petroleum ether, petroleum ether, chloroform, ethyl acetate, butanols and the ethanol to carry 1～24 hour, filtering extracting solution, extracting solution concentrated remove solvent, promptly get fat-soluble live part extract; The w/v of described raw material and solvent is: 1g: 6～15ml, and described fat-soluble live part extract is 0.8000～0.9000 20 ℃ of relative densities; Index of refraction is 1.2500～1.4000 at D20 ℃.
Two the technical scheme that realizes the object of the invention is: the extracting method of lipophilic effective extract of Chinese green onion, it is after one or more the combination in exsiccant very light blue or dried Sucus Allii Fistulosi or the dry full Herba Alii fistulosi is pulverized, adopt a kind of solvent cold soak or heat in ether, petroleum ether, chloroform, ethyl acetate, butanols and the ethanol to carry 1～24 hour, filtering extracting solution, extracting solution concentrated remove solvent, promptly get fat-soluble live part extract; The w/v of described raw material and solvent is: 1g: 6～15ml.
It is to adopt petroleum ether (30～60 ℃) to extract.
It is to adopt a kind of solvent in petroleum ether (60～90 ℃), chloroform, ethyl acetate, butanols and the ethanol to extract.
Described extracting solution concentrates and to remove solvent and be: be evaporated to no above-mentioned solvent at 25～60 ℃ of temperature, pressure 0.08～0.095Mpa and distinguish the flavor of.
Main component contains volatile oil, steroid saponin compound, chromocor compound, vitamin, polysaccharide etc. in the described liposoluble effective extracts.Nature and flavor suffering, temperature; Function is for delivering, activate yang, detoxifying.Be used for the headache of typhoid fever cold and heat, YIN-cold stomachache, the malnutrition due to parasitic infestation internal resistance, constipation and anuresis, dysentery, pain is swollen.Aspects such as cardiovascular disease there is significant curative effect.Its extracting method is simple, and is easy to control, stable.
The specific embodiment
This extract is the liposoluble effective extracts of shallot Allium fisturosum L.var.caespi-tosumMakio or chive Allium schoenoprasum L., and it can be above-mentioned fresh bulb or bright Sucus Allii Fistulosi or fresh full Herba Alii fistulosi, after drying, passes through CO again
2The supercritical extraction separation and Extraction.Drying wherein very light blue or Sucus Allii Fistulosi or full Herba Alii fistulosi can adopt micro-wave drying method; The temperature that is microwave drying is 20～60 ℃; The microwave drying time is 15min～5h, and vacuum is 0.085～0.095Mpa.Also can adopt: freeze-drying; Promptly at-30～-50 ℃ of pre-freeze 24h, dividing plate to 0 ℃ dry 12h is heightened then with-30～-50 ℃ of dry 12h in the back, is warming up to 10 ℃ of dry 6h after finishing again, gives birth to 20 ℃ of dry 6h at last.
After one or more combination in above-mentioned exsiccant very light blue or dried Sucus Allii Fistulosi or the dry full Herba Alii fistulosi pulverized, extract with solvent.
Embodiment 1
Use ether 60～150ml merceration after 1～24 hour above-mentioned powder 10g, filtering extracting solution is evaporated to no above-mentioned solvent flavor with extracting solution at 25～60 ℃ of temperature, pressure 0.08～0.095Mpa, promptly gets fat-soluble live part extract.
The consumption of above-mentioned solvent ether can be according to production requirement, can select between 60～150ml to select arbitrarily any interval value within some points such as 60ml, 80ml, 88ml, 95.6ml, 160ml or the 60～150ml, as the consumption between 65～130ml, the 60～100ml etc.; The described merceration time can select some points or the interval value within 1～24 hour to select arbitrarily between 1～24 hour.
Embodiment 2
After above-mentioned powder 10g extracted 1～24 hour with petroleum ether 60～150ml of 30～60 ℃, filtering extracting solution, extracting solution is evaporated to no above-mentioned solvent flavor at 25～60 ℃ of temperature, pressure 0.08～0.095Mpa, promptly gets fat-soluble live part extract.
A kind of conventional requirement during said extracted can adopt merceration or seepage or heat to carry to wait is carried out; The consumption of petroleum ether can be selected any some points between 60～150ml, as 60ml, 80ml, 88ml, 95.6ml, 160ml etc. or be chosen in any interval value within 60～150ml, and as 60～120ml, 70～140ml etc.
Embodiment 3
After above-mentioned powder 10g extracted 1～24 hour with a kind of 60～150ml in the petroleum ether of ethanol, chloroform, ethyl acetate, butanols and 60～90 ℃, filtering extracting solution, extracting solution is evaporated to no above-mentioned solvent flavor at 25～60 ℃ of temperature, pressure 0.08～0.095Mpa, promptly gets fat-soluble live part extract.
A kind of conventional requirement during said extracted can adopt merceration or seepage or heat to carry to wait is carried out, the consumption of solvent can be selected any some points between 60～150ml, as 65ml, 75ml, 90ml, 100ml, 150ml or be chosen in interval value within 60～150ml, as 70～100ml, 76～160ml etc.
The spissated temperature of extracting solution can be selected some points arbitrarily in the foregoing description between 25～60 ℃, and pressure can be selected some points arbitrarily between 0.08～0.095Mpa.
The filtration of extracting solution can be adopted one or many, after the multiple times of filtration, the filtering filtrate of each time is mixed the back reconcentration remove solvent.
Use it for anything else fully through extracting the filtrate of filtering gained in the foregoing description.
The said extracted thing is measured:
The physicochemical constant of fat-soluble effective site is through the repeated detection analysis:
(1) extracts percentage rate (g/g): 1.20%～6%
(2) color and luster: yellowish-brown
(3) relative density (20 ℃): 0.8000～0.9000 interval interior any value.
(4) index of refraction (D20 ℃): 1.2500～1.4500 interval interior any values.
Fat-soluble effective site volatile oil contents is measured
Adopt the assay method of Chinese Pharmacopoeia volatile oil to measure
Sample determination: precision is measured quantity of sample, presses 57 pages of mensuration of 2005 editions appendix of Chinese Pharmacopoeia, and volatile oil content is 0.01～0.05%.
The assay of steroid sapogenin
Adopt colorimetry to measure sapogenin constituents content, the reaction of main at present employing anisaldehyde is carried out quantitatively, is that standard substance are found out sample and standard substance maximum absorption band by UV scanning with the Sarsasapogenin.And, make standard curve with the Sarsasapogenin standard substance at the maximum absorption band place, come the content of steroid sapogenin constituents in the working sample with this.
Key instrument and reagent
CARY 50 Scan UV-Visible Spectrophotometer；
Anisaldehyde, methanol, concentrated hydrochloric acid, concentrated sulphuric acid, petroleum ether (60～90 ℃) and dehydrated alcohol are analytical pure;
The Sarsasapogenin reference substance
The mensuration of maximum absorption band
Accurately draw a certain amount of Sarsasapogenin standard solution, volatilize solvent, and adding 0.5ml anisaldehyde solution (anisaldehyde: concentrated sulphuric acid: dehydrated alcohol=0.1: 1: 18.9), boiling water bath 15min, ice bath 10min adds methanol constant volume to 10ml.Thereby in 200～700nm scope, carry out UV scanning by ultraviolet scanner and determine its maximum absorption band.In kind sample solution is done chromogenic reaction; Compare then, sample and standard substance all are equipped with absorption maximum in 420nm place identical bits.
The assay of steroid sapogenin constituents
The preparation of reference substance solution: precision takes by weighing 2mg Sarsasapogenin standard substance, uses dissolve with methanol, and is fixed molten to the 2ml volumetric flask, standby.
The preparation of standard curve: draw 0.15ml, 0.20ml, 0.25ml, 0.30ml, 0.35ml standard solution respectively, add methanol constant volume to 10ml, measure as stated above, get standard curve linear equation Y=0.7946x+0.1229 R2=0.9980, the result shows: Sarsasapogenin is good in 0.015～0.035mg scope internal linear relation.
| Sample volume (ml) | 0.15 | 0.20 | 0.25 | 0.30 | 0.35 |
| Concentration (mg/ml) | 0.015 | 0.020 | 0.025 | 0.030 | 0.035 |
| Absorbance | 0.239 | 0.286 | 0.322 | 0.361 | 0.400 |
The assay of sample
Precision is measured quantity of sample solution, measures its absorbance as stated above, and the standard curve by Sarsasapogenin calculates sample size, and steroid sapogenin contains 15～30% in the sample
Lipophilic effective extract of Chinese green onion has high medical value.
Claims (10)
1, a kind of extracting method of lipophilic effective extract of Chinese green onion, it is after one or more the combination in exsiccant very light blue or dried Sucus Allii Fistulosi or the dry full Herba Alii fistulosi is pulverized, adopt a kind of solvent cold soak or heat in ether, petroleum ether, petroleum ether, chloroform, ethyl acetate, butanols and the ethanol to carry 1～24 hour, filtering extracting solution, extracting solution concentrated remove solvent, promptly get fat-soluble live part extract; The w/v of described raw material and solvent is: 1g: 6～15ml.
2, the extracting method of lipophilic effective extract of Chinese green onion according to claim 1, it is characterized in that described extracting solution concentrates removes solvent and is: be evaporated to no above-mentioned solvent at 25～60 ℃ of temperature, pressure 0.08～0.095Mpa and distinguish the flavor of.
3, the extracting method of lipophilic effective extract of Chinese green onion according to claim 1 is characterized in that it is to adopt 30～60 ℃ petroleum ether to extract.
4, the extracting method of lipophilic effective extract of Chinese green onion according to claim 1 is characterized in that it is to adopt 60～90 ℃ petroleum ether solvent to extract.
5, a kind of lipophilic effective extract of Chinese green onion, it is after one or more the combination in exsiccant very light blue or dried Sucus Allii Fistulosi or the dry full Herba Alii fistulosi is pulverized, the combination solvent merceration or the heat of one or more in employing ether, petroleum ether, petroleum ether, chloroform, ethyl acetate, butanols and the ethanol are carried 1～24 hour, filtering extracting solution, extracting solution concentrated remove solvent, promptly get fat-soluble live part extract; The w/v of described raw material and solvent is: 1g: 6～15ml, and described fat-soluble live part extract is 0.8000～0.9000 20 ℃ of relative densities; Index of refraction is 1.2500～1.4500 at D20 ℃.
6, as lipophilic effective extract of Chinese green onion as described in the claim 5, it is characterized in that: described extracting solution concentrates and removes solvent and be: be evaporated to no above-mentioned solvent at 25～60 ℃ of temperature, pressure 0.08～0.095Mpa and distinguish the flavor of.
7,, it is characterized in that it is to adopt 30～60 ℃ petroleum ether to extract to obtain as lipophilic effective extract of Chinese green onion as described in the claim 5.
8,, it is characterized in that it is to adopt 60～90 ℃ petroleum ether solvent to extract to obtain as lipophilic effective extract of Chinese green onion as described in the claim 5.
9, the extracting method of lipophilic effective extract of Chinese green onion or as lipophilic effective extract of Chinese green onion as described in the claim 5 according to claim 1 is characterized in that the drying means of described exsiccant very light blue or dried Sucus Allii Fistulosi or dry full Herba Alii fistulosi is: micro-wave drying method; The temperature of described micro-wave drying method is 20～60 ℃; The microwave drying time is 15min～5h, and vacuum is 0.085～0.095Mpa.
10, the extracting method of lipophilic effective extract of Chinese green onion or as lipophilic effective extract of Chinese green onion as described in the claim 5 according to claim 1 is characterized in that the drying means of described exsiccant very light blue or dried Sucus Allii Fistulosi or dry full Herba Alii fistulosi is: freeze-drying; Described freeze-drying is: at-30～-50 ℃ of pre-freeze 24h, dividing plate to 0 ℃ dry 12h is heightened then with-30～-50 ℃ of dry 12h in the back, is warming up to 10 ℃ of dry 6h after finishing again, gives birth to 20 ℃ of dry 6h at last.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100195378A CN101095814A (en) | 2006-06-30 | 2006-06-30 | Liposoluble effective extracts from onion white and the extracting method thereof |
| PCT/CN2007/001947 WO2008003226A1 (en) | 2006-06-30 | 2007-06-21 | Caulis allii fistulosum extract and the use in modulating blood-lipids and lowering cholesterols thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100195378A CN101095814A (en) | 2006-06-30 | 2006-06-30 | Liposoluble effective extracts from onion white and the extracting method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN101095814A true CN101095814A (en) | 2008-01-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2006100195378A Pending CN101095814A (en) | 2006-06-30 | 2006-06-30 | Liposoluble effective extracts from onion white and the extracting method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919437A (en) * | 2010-09-26 | 2010-12-22 | 福建农林大学 | Scallion microwave vacuum drying technology |
| CN103181401A (en) * | 2013-02-26 | 2013-07-03 | 安徽师范大学 | Algistat |
| CN103229857A (en) * | 2013-04-25 | 2013-08-07 | 西华大学 | Quenching and extracting process of onion oil and garlic oil |
-
2006
- 2006-06-30 CN CNA2006100195378A patent/CN101095814A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101919437A (en) * | 2010-09-26 | 2010-12-22 | 福建农林大学 | Scallion microwave vacuum drying technology |
| CN103181401A (en) * | 2013-02-26 | 2013-07-03 | 安徽师范大学 | Algistat |
| CN103229857A (en) * | 2013-04-25 | 2013-08-07 | 西华大学 | Quenching and extracting process of onion oil and garlic oil |
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Open date: 20080102 |