CN101085191B - Pulse engendendering active component extraction and its preparation - Google Patents
Pulse engendendering active component extraction and its preparation Download PDFInfo
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- CN101085191B CN101085191B CN2006100142281A CN200610014228A CN101085191B CN 101085191 B CN101085191 B CN 101085191B CN 2006100142281 A CN2006100142281 A CN 2006100142281A CN 200610014228 A CN200610014228 A CN 200610014228A CN 101085191 B CN101085191 B CN 101085191B
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Abstract
The invention relates to a medicinal composition of effective positions for restoring pulse beat and its preparation comprising the following raw materials (by weight percent): ginseng saponin 15-90%, ophiopogon root total saponin 2-55%, schisandra fruit lignans 2-55%. The composition can be mixed with one or more pharmacological auxiliary materials to obtain various dose forms including injections, tablets, slow release capsules, dripping pills, granules, powder injection, capsules and fine granules.
Description
Technical field
The invention belongs to pharmaceutical product, specifically, relate to pulse engendendering active component extraction and preparation thereof.
Background technology
Present existing formulation of ' Sheng Mai ' all is to be that raw material is made by Radix Ginseng Rubra, Radix Ophiopogonis and Fructus Schisandrae Chinensis, this side comes from the SHENGMAI YIN of Jin Dynasty Li Gao " differentiation on endogenous ", the clinical syndrome that is widely used in deficiency of both QI and YIN all has good curative effect to acute stage, septic shock and other multiple critical illness such as the stable phase of patients with coronary heart disease, acute myocardial infarction, cardiogenic shock, arrhythmia.Also can be used as the paramedicines of treatment cancer, be used to alleviate the untoward reaction that chemotherapy, radiotherapy cause.But there is the extraction process imperfection in existing formulation of ' Sheng Mai ', extracts active ingredients is incomplete, the control of product quality difficulty is big, low and the imperfection of preparation quality standard, the Tween class cosolvent that has for example used the medication of painstaking effort tubing to add in the pulse restoring injection, drug level is low, and clinical consumption is big, and transports, stores also all inconvenient.Therefore, provide the preparation of convenient effective pulse engendendering active component extraction to have great importance.
Summary of the invention
The object of the present invention is to provide a kind of quality controllable, stable high pulse engendendering active component extraction and preparation thereof.
The present invention is implemented by following technical proposals.
Pulse engendendering active component extraction of the present invention, form with Radix Ginseng, Radix Ophiopogonis and Fructus Schisandrae Chinensis mixed extraction, contain Radix Ginseng total saponins 15%～90% by weight percentage, Radix Ophiopogonis total saponins 2%～55%, Fructus Schisandrae Chinensis total lignanoid 2%～55%, three's sum are 70%～99%; Be preferably the Radix Ginseng total saponins 30%～70% that contains by weight percentage, Radix Ophiopogonis total saponins 5%～35%, Fructus Schisandrae Chinensis total lignanoid 5%～30%, three's sum is 70%～99%; More preferably contain Radix Ginseng total saponins 45%～65% by weight percentage, Radix Ophiopogonis total saponins 7%～25%, Fructus Schisandrae Chinensis total lignanoid 7%～25%, three's sum is 70%～99%.
Pulse engendendering active component extraction of the present invention can obtain with prior art, but needs the refining content standard that reaches above-mentioned Radix Ginseng total saponins, Radix Ophiopogonis total saponins, Fructus Schisandrae Chinensis total lignanoid, but specific method also of the present invention is prepared.
The preparation of pulse engendendering active component extraction of the present invention:
Get Radix Ginseng or Folium Ginseng medical material, Radix Ophiopogonis medical material and Fructus Schisandrae Chinensis three flavor medical materials by a certain percentage, mix, adding water or 40～80% ethanol, is 4～6 with alkali regulator solution pH value, heating or supersound extraction 2～4 times, merge extractive liquid,, filter, the macroporous type alkali anion exchange column of having handled well on the filtrate washes with aqueous alkali earlier, pure water rinsing is used in the back, and flushing liquor discards; Reuse 50～95% ethanol carry out eluting, collect eluent, and eluent is concentrated into thick paste, and drying gets pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 15%～90% by weight percentage, Radix Ophiopogonis total saponins 2%～55%, Fructus Schisandrae Chinensis total lignanoid 2%～55%, wherein the mensuration of Radix Ginseng total saponins is referring to document (Anhui Chinese Medicine College journal, in August, 2003,22 (4): 51), the mensuration of Radix Ophiopogonis total saponins is referring to document (Chinese herbal medicine, in December, 2003,34 (12): 1090), the mensuration of Fructus Schisandrae Chinensis total lignanoid is referring to document (northwest pharmaceutical journal, in October, 1999,14 (5): 197).
Pulse engendendering active component extraction of the present invention, the various dosage forms that can be mixed and made into adjuvant on any or more than one pharmaceuticss such as starch, dextrin, lactose, microcrystalline Cellulose, hydroxypropyl methylcellulose, Polyethylene Glycol, magnesium stearate, micropowder silica gel, xylitol, lactose, glucose, glycine, mannitol, glycine, HP-etc., for example, can be made into injection, tablet, slow releasing tablet, drop pill, granule, injectable powder, capsule, microgranule.Preferred dosage form is injectable powder, drop pill, capsule.
Clinical diseases such as heart source property, septic shock, cardiomyopathy, coronary heart disease, the more remarkable treatment effect of being used for the treatment of of the present invention.Pharmacological evaluation shows: compare with existing formulation of ' Sheng Mai ', the Acute Myocardial Ischemia in Rats that pulse engendendering active component extraction of the present invention and preparation thereof cause pituitrin has tangible antagonism (P＜0.01), non-specific antiinflammatory action is higher, the cellular immunization and the humoral immune function of obvious enhancing body, obviously improve body to anoxybiotic tolerance, and toxicity is little, does not have tangible irritative response.
Raw material sources of the present invention are easy to get, and are easy to industrialization; Can make various dosage forms as required, for clinical provide convenient, more effectively, the more controlled modern Chinese medicine of quality, for the patient brings more benefits, thereby produce the huge social benefit.
The specific embodiment
To be easier to understand the present invention with reference to the following example, and provide embodiment and be in order to illustrate the present invention, rather than in order to limit the scope of the invention.
The preparation of embodiment arteries and veins effective part extract in all one's life
Method 1:
Ratio in 1: 0.9: 0.1 weight portion takes by weighing Folium Ginseng, Radix Ophiopogonis and schisandra chinensis medicinal material, mix homogeneously, add water boil extraction three times and control acid-base value, for the first time be the Na2CO3 of the 6 times of water yields and the 1.1% medical material amount of medical material amount, for the second time being the Na2CO3 of the 4 times of water yields and the 0.7% medical material amount of medical material amount, is 4 times of water yields of medical material amount for the third time; The side circulated in countercurrent is extracted, and keeps respectively after boiling 1.5,1,1 hours; The medicinal liquid merging is chilled to room temperature, filters, and the macroporous type weakly-basic anion D392 exchange column of having handled well on the medicinal liquid filtrate (Chemical Plant of Nankai Univ.), with the 2%NaOH solution flushing of 2 times of resin bed volumes, flushing liquor discards; 3 times of pure water rinsing again, aqueous rinse solution discards; With 70% ethanol elution of 7 times of resin bed volumes, collect eluent, be concentrated into thick paste (50 ℃ of following relative densities are 1.28～1.33), with dynamic belt drying equipment drying, get pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 77.3% by weight percentage, Radix Ophiopogonis total saponins 6.4%, Fructus Schisandrae Chinensis total lignanoid 2.4%.
Method 2:
Ratio in 1: 2.2: 0.3 weight portion takes by weighing Radix Ginseng Rubra, Radix Ophiopogonis and schisandra chinensis medicinal material, mix homogeneously, add water boil extraction three times and control acid-base value, for the first time be the Na2CO3 of the 5 times of water yields and the 1.1% medical material amount of medical material amount, for the second time being the Na2CO3 of the 4 times of water yields and the 0.8% medical material amount of medical material amount, is 4 times of water yields of medical material amount for the third time; The side circulated in countercurrent is extracted, and keeps respectively after boiling 1.5,1,1 hours; The medicinal liquid merging is chilled to room temperature, filters, and the macroporous type weakly-basic anion D380 exchange column of having handled well on the medicinal liquid filtrate (Chemical Plant of Nankai Univ.), with the 2%NaOH solution flushing of 2 times of resin bed volumes, flushing liquor discards; 3 times of pure water rinsing again, aqueous rinse solution discards; With 80% ethanol elution of 7 times of resin bed volumes, collect eluent, be concentrated into thick paste (50 ℃ of following relative densities are 1.28～1.33), with dynamic belt drying equipment drying, get pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 64.8% by weight percentage, Radix Ophiopogonis total saponins 13.8%, Fructus Schisandrae Chinensis total lignanoid 6.3%.
Method 3:
Ratio in 1: 3.0: 1.5 weight portion takes by weighing Radix Ginseng Rubra, Radix Ophiopogonis and schisandra chinensis medicinal material, mix homogeneously, add water boil extraction three times and control acid-base value, for the first time be the NaHCO3 of the 6 times of water yields and the 1.2% medical material amount of medical material amount, for the second time being the NaHCO3 of the 4 times of water yields and the 0.7% medical material amount of medical material amount, is 4 times of water yields of medical material amount for the third time; The side circulated in countercurrent is extracted, and keeps respectively after boiling 1.5,1,1 hours; The medicinal liquid merging is chilled to room temperature, filters, and the macroporous type strong alkalinity anion D201 exchange column of having handled well on the medicinal liquid filtrate (Chemical Plant of Nankai Univ.), with the 2%NaOH solution flushing of 2 times of resin bed volumes, flushing liquor discards; 3 times of pure water rinsing again, aqueous rinse solution discards; With 65% ethanol elution of 7 times of resin bed volumes, collect eluent, be concentrated into thick paste (50 ℃ of following relative densities are 1.28～1.33), with dynamic belt drying equipment drying, get pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 44.5% by weight percentage, Radix Ophiopogonis total saponins 12.8%, Fructus Schisandrae Chinensis total lignanoid 23.7%.
The preparation of embodiment two pulse engendendering active component extractions
Method 1:
Ratio in 1: 1.4: 0.7 weight portion takes by weighing Radix Ginseng Rubra, Radix Ophiopogonis and schisandra chinensis medicinal material, mix homogeneously, add water supersound extraction four times and control acid-base value, for the first time be the Na2CO3 of the 5 times of water yields and the 1.5% medical material amount of medical material amount, for the second time being the Na2CO3 of the 4 times of water yields and the 0.7% medical material amount of medical material amount, is 3 times of water yields of medical material amount with the 4th time for the third time; The medicinal liquid merging is chilled to room temperature, filters, and the macroporous type weakly-basic anion D382 exchange column of having handled well on the medicinal liquid filtrate (Chemical Plant of Nankai Univ.), with the 2.5%NaOH solution flushing of 1.5 times of resin bed volumes, flushing liquor discards; 4 times of pure water rinsing again, aqueous rinse solution discards; With 75% ethanol elution of 8 times of resin bed volumes, collect eluent, be concentrated into thick paste (50 ℃ of following relative densities are 1.28～1.33), with dynamic belt drying equipment drying, get pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 59.9% by weight percentage, Radix Ophiopogonis total saponins 8.2%, Fructus Schisandrae Chinensis total lignanoid 15.0%.
Method 2:
Ratio in 1: 4.8: 0.2 weight portion takes by weighing Folium Ginseng, Radix Ophiopogonis and schisandra chinensis medicinal material, mix homogeneously, add water supersound extraction four times and control acid-base value, for the first time be the NaHCO3 of the 5 times of water yields and the 1.6% medical material amount of medical material amount, for the second time being the NaHCO3 of the 4 times of water yields and the 0.8% medical material amount of medical material amount, is 3 times of water yields of medical material amount with the 4th time for the third time; The medicinal liquid merging is chilled to room temperature, filters, and the macroporous type strong alkalinity anion D201 exchange column of having handled well on the medicinal liquid filtrate (Chemical Plant of Nankai Univ.), with the 2.5%NaOH solution flushing of 1.5 times of resin bed volumes, flushing liquor discards; 4 times of pure water rinsing again, aqueous rinse solution discards; With 85% ethanol elution of 8 times of resin bed volumes, collect eluent, be concentrated into thick paste (50 ℃ of following relative densities are 1.28～1.33), with dynamic belt drying equipment drying, get pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 55.1% by weight percentage, Radix Ophiopogonis total saponins 25.5%, Fructus Schisandrae Chinensis total lignanoid 3.9%.
Method 3:
Ratio in 1: 4.1: 0.7 weight portion takes by weighing Radix Ginseng Rubra, Radix Ophiopogonis and schisandra chinensis medicinal material, mix homogeneously, add water supersound extraction four times and control acid-base value, for the first time be the NaHCO3 of the 5 times of water yields and the 1.5% medical material amount of medical material amount, for the second time being the NaHCO3 of the 4 times of water yields and the 0.8% medical material amount of medical material amount, is 3 times of water yields of medical material amount with the 4th time for the third time; The medicinal liquid merging is chilled to room temperature, filters, and the macroporous type weakly-basic anion D371 exchange column of having handled well on the medicinal liquid filtrate (Chemical Plant of Nankai Univ.), with the 2.5%NaOH solution flushing of 1.5 times of resin bed volumes, flushing liquor discards; 4 times of pure water rinsing again, aqueous rinse solution discards; With 70% ethanol elution of 8 times of resin bed volumes, collect eluent, be concentrated into thick paste (50 ℃ of following relative densities are 1.28～1.33), with dynamic belt drying equipment drying, get pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 51.2% by weight percentage, Radix Ophiopogonis total saponins 20.0%, Fructus Schisandrae Chinensis total lignanoid 11.8%.The preparation of embodiment three lives arteries and veins effective part extract
Method 1:
Ratio in 1: 6.9: 0.2 weight portion takes by weighing Folium Ginseng, Radix Ophiopogonis and schisandra chinensis medicinal material, mix homogeneously, add 50% alcohol reflux three times and control acid-base value, for the first time be the Na2CO3 of 5 times of 50% amount of alcohol and the 1.0% medical material amount of medical material amount, for the second time being the Na2CO3 of 4 times of 50% amount of alcohol and the 0.6% medical material amount of medical material amount, is 4 times of 50% amount of alcohol of medical material amount for the third time; Side circulated in countercurrent reflux, extract, kept respectively 1.5,1,1 hours after the beginning that refluxes; Medical filtration and merging filtrate, be evaporated under 70 ℃ of the filtrates do not have the alcohol flavor after, concentrated solution thin up to medical material medicinal liquid ratio is 1: 1.5, the macroporous type weakly-basic anion D382 exchange column of having handled well on the diluent (Chemical Plant of Nankai Univ.), with the 2%NaOH solution flushing of 2 times of resin bed volumes, flushing liquor discards; 3 times of pure water rinsing again, aqueous rinse solution discards; With 70% ethanol elution of 7 times of resin bed volumes, collect eluent, be concentrated into thick paste (50 ℃ of following relative densities are 1.28～1.33), with dynamic belt drying equipment drying, get pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 48.3% by weight percentage, Radix Ophiopogonis total saponins 31.5%, Fructus Schisandrae Chinensis total lignanoid 3.9%.
Method 2:
Ratio in 1: 2.2: 3.7 weight portion takes by weighing Radix Ginseng Rubra, Radix Ophiopogonis and schisandra chinensis medicinal material, mix homogeneously, add 60% ethanol ultrasonic extraction three times and control acid-base value, for the first time be the Na2CO3 of 5 times of 50% amount of alcohol and the 1.1% medical material amount of medical material amount, for the second time being the Na2CO3 of 4 times of 60% amount of alcohol and the 0.7% medical material amount of medical material amount, is 4 times of 60% amount of alcohol of medical material amount for the third time; The medicinal liquid merging is chilled to room temperature, filter, be evaporated under 70 ℃ of the filtrates do not have the alcohol flavor after, concentrated solution thin up to medical material medicinal liquid ratio is 1: 1.5, the macroporous type weakly-basic anion D392 exchange column of having handled well on the diluent (Chemical Plant of Nankai Univ.), with the 2%NaOH solution flushing of 2 times of resin bed volumes, flushing liquor discards; 3 times of pure water rinsing again, aqueous rinse solution discards; With 80% ethanol elution of 7 times of resin bed volumes, collect eluent, be concentrated into thick paste (50 ℃ of following relative densities are 1.28～1.33), with dynamic belt drying equipment drying, get pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 30.9% by weight percentage, Radix Ophiopogonis total saponins 6.4%, Fructus Schisandrae Chinensis total lignanoid 40.2%.
Method 3:
Ratio in 1: 25.6: 2.7 weight portion takes by weighing Radix Ginseng Rubra, Radix Ophiopogonis and schisandra chinensis medicinal material, mix homogeneously, add 65% alcohol reflux three times and control acid-base value, for the first time be the NaHCO3 of 5 times of 65% amount of alcohol and the 1.5% medical material amount of medical material amount, for the second time being the NaHCO3 of 4 times of 65% amount of alcohol and the 0.7% medical material amount of medical material amount, is 4 times of 50% amount of alcohol of medical material amount for the third time; Side circulated in countercurrent reflux, extract, kept respectively 1.5,1,1 hours after the beginning that refluxes; Medical filtration and merging filtrate, be evaporated under 70 ℃ of the filtrates do not have the alcohol flavor after, concentrated solution thin up to medical material medicinal liquid ratio is 1: 1.5, the macroporous type weakly-basic anion D380 exchange column of having handled well on the diluent (Chemical Plant of Nankai Univ.), with the 2%NaOH solution flushing of 2 times of resin bed volumes, flushing liquor discards; 3 times of pure water rinsing again, aqueous rinse solution discards; With 75% ethanol elution of 7 times of resin bed volumes, collect eluent, be concentrated into thick paste (50 ℃ of following relative densities are 1.28～1.33), with dynamic belt drying equipment drying, get pulse engendendering active component extraction.This pulse engendendering active component extraction contains Radix Ginseng total saponins 19.3% by weight percentage, Radix Ophiopogonis total saponins 47.4%, Fructus Schisandrae Chinensis total lignanoid 14.2%.
Embodiment four pulse engendendering active component injectable powder
Get pulse engendendering active component extraction (embodiment one method 1) 9g, add 5 times of waters for injection, after heating makes dissolving under stirring, first while hot coarse filtration, filtrate for later use; HP-225g adds an amount of water for injection, after heating makes dissolving, filters while hot; Filtrate is mixed with medicinal liquid, and 60 ℃ are stirred after 60 minutes down, stop heating; Add mannitol 115g, stir and make dissolving, adjust pH to 6.5～7.0 add the injection water to cumulative volume, continue to stir after 10 minutes, are chilled to room temperature, aseptic filtration.The filtrate fill is in cillin bottle, and lyophilization, is added a cover, promptly at tamponade.
Embodiment five pulse engendendering active component injectable powder
Get pulse engendendering active component extraction (embodiment two methods 1) 9g, add lecithin and each 115g of mannitol, an amount of water for injection, after heating made dissolving, adjust pH to 6.5～7.0 added the injection water to cumulative volume, stir 10 minutes, be chilled to room temperature after, aseptic filtration; The filtrate fill is in cillin bottle, and lyophilization, is added a cover, promptly at tamponade.
Embodiment six pulse engendendering active component injectable powder
Get pulse engendendering active component extraction (embodiment one method 3) 9g, add lecithin and each 115g of mannitol, an amount of water for injection, after heating made dissolving, adjust pH to 6.5～7.0 added the injection water to cumulative volume, stir 10 minutes, be chilled to room temperature after, aseptic filtration; The filtrate fill is in cillin bottle, and lyophilization, is added a cover, promptly at tamponade.
Embodiment seven pulse engendendering active component injections
Get pulse engendendering active component extraction (embodiment two methods 2) 9g, add 5 times of waters for injection, after heating makes dissolving under stirring, first while hot coarse filtration, filtrate for later use; HP-225g adds an amount of water for injection, after heating makes dissolving, filters while hot; Filtrate is mixed with medicinal liquid, and 60 ℃ are stirred after 60 minutes down, stop heating; Add mannitol 115g, stir and make dissolving, adjust pH to 6.5～7.0 add the injection water to cumulative volume, continue to stir after 10 minutes, are chilled to room temperature, and aseptic filtration promptly gets injection.
Embodiment eight pulse engendendering active component capsules
Get pulse engendendering active component extraction (embodiment two methods 3) 9g,, add 3% polyvidone alcoholic solution system soft material, cross 18 mesh sieve system granules with 40g microcrystalline Cellulose mix homogeneously, 600 ℃ of dryings 30～45 minutes, granulate adds the 4g Pulvis Talci, mixing fills in capsule, promptly.
Embodiment nine pulse engendendering active component tablets
Get pulse engendendering active component extraction (embodiment three methods 1) 9g,, add 3% polyvidone alcoholic solution system soft material, cross 18 mesh sieve system granules with 40g microcrystalline Cellulose mix homogeneously, 600 ℃ of dryings 30～45 minutes, granulate adds the 4g Pulvis Talci, mixing, mixing, tabletting, promptly.
Embodiment ten pulse engendendering active component granules
Get pulse engendendering active component extraction (embodiment three methods 2) 9g,, add 3% polyvidone alcoholic solution system soft material, cross 18 mesh sieve system granules with 40g microcrystalline Cellulose mix homogeneously, 600 ℃ of dryings 30～45 minutes, granulate adds the 4g Pulvis Talci, mixing, granulate, pack, promptly.
Embodiment arteries and veins effective site drop pill in ten all one's life
Pulse engendendering active component extraction (embodiment three methods 3) 9g, with 700g Polyethylene Glycol-6000 mix homogeneously, fusion splashes in the cryogenic liquid paraffin, selects ball, removes liquid paraffin, promptly.
Embodiment 12 pulse engendendering active component drop pills
Get pulse engendendering active component extraction (embodiment one method 2) 9g, with 250g Polyethylene Glycol-4000 and 450g Polyethylene Glycol-6000 mix homogeneously, fusion splashes in the low temperature methyl-silicone oil, selects ball, removes methyl-silicone oil, promptly.
Claims (7)
1. pulse engendendering active component extraction, it is to use Radix Ginseng, Radix Ophiopogonis and Fructus Schisandrae Chinensis mixed extraction form, contain Radix Ginseng total saponins 15%～90% by weight percentage, Radix Ophiopogonis total saponins 2%～55%, Fructus Schisandrae Chinensis total lignanoid 2%～55%, three's sum is 70%～99%, it is characterized in that, it is to be prepared from by following method: get Radix Ginseng or Folium Ginseng medical material by a certain percentage, Radix Ophiopogonis medical material and Fructus Schisandrae Chinensis three flavor medical materials, mix, adding water or 40～80% ethanol, is to extract after 4～6 with alkali regulator solution pH value again, and extracting solution filters, the macroporous type alkali anion exchange column of having handled well on the filtrate, with the aqueous alkali flushing, pure water rinsing is used in the back earlier, and flushing liquor discards; Reuse 50～95% ethanol carry out eluting, collect eluent, and eluent is concentrated into thick paste, drying, promptly.
2. extract according to claim 1 is characterized in that, it contains Radix Ginseng total saponins 30%～70% by weight percentage, Radix Ophiopogonis total saponins 5%～35%, and Fructus Schisandrae Chinensis total lignanoid 5%～30%, three's sum is 70%～99%.
3. extract according to claim 1 is characterized in that, it contains Radix Ginseng total saponins 45%～65% by weight percentage, Radix Ophiopogonis total saponins 7%～25%, and Fructus Schisandrae Chinensis total lignanoid 7%～25%, three's sum is 70%～99%.
4. according to claim 1,2 or 3 described extracts, it is characterized in that described is with Na2CO3 or NaHCO3 regulator solution pH value with alkali regulator solution pH value.
5. extract according to claim 4 is characterized in that, described heating or supersound extraction 2～4 times, the merge extractive liquid, of being extracted as.
6. extract according to claim 4, it is characterized in that described macroporous type alkali anion exchange column is macroporous type weakly-basic anion D392 exchange column, macroporous type weakly-basic anion D380 exchange column, macroporous type weakly-basic anion D382 exchange column, macroporous type weakly-basic anion D371 exchange column or macroporous type strong alkalinity anion D201 exchange column.
7. extract according to claim 4 is characterized in that, the concentration of alcohol of described ethanol elution is 60%～85%.
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| CN101744989B (en) * | 2008-12-05 | 2014-01-29 | 天津天士力之骄药业有限公司 | Method for extracting schisandra chinensis, ginseng and dwarf lilyturf root and preparation thereof |
| CN101744988B (en) * | 2008-12-05 | 2013-10-16 | 天津天士力之骄药业有限公司 | Method for extracting ginseng, dwarf lilyturf tuber and schisandra chinensis and preparation thereof |
| CN106074866A (en) * | 2016-07-05 | 2016-11-09 | 江苏苏南药业实业有限公司 | The Chinese medicine preparation of a kind of resisting cardiovascular disease and application |
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| CN1265911A (en) * | 1999-03-09 | 2000-09-13 | 中国药科大学 | Medicine for treating cardiovascular disease and preparation process thereof |
| CN1593621A (en) * | 2004-06-22 | 2005-03-16 | 张晴龙 | Orally disintegrating tablet of 'Shengmai' and its preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1265911A (en) * | 1999-03-09 | 2000-09-13 | 中国药科大学 | Medicine for treating cardiovascular disease and preparation process thereof |
| CN1593621A (en) * | 2004-06-22 | 2005-03-16 | 张晴龙 | Orally disintegrating tablet of 'Shengmai' and its preparation |
| CN1739735A (en) * | 2005-09-23 | 2006-03-01 | 张文芳 | Chinese medicine effective part composition |
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| 岳磊等.生脉散制剂中总皂苷含量的测定.《中成药》.2004,第26卷(第11期),897-900. * |
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