实施例2:ChimeriVax
TM
-JE和ChimeriVax
TM
-DEN1-4
背景和概述
在下面描述的试验中,我们用在无血清(SF)培养基中生长的非洲绿猴肾细胞制备并定性了新的ChimeriVaxTM-JE种病毒(seed virus),来消除对于疫苗可能被牛传播性脑病(bovine transmissible encephalopathy)的朊病毒因子(agent)污染的任何顾虑。在于SF培养物中增殖的过程中,未克隆的病毒累积了在含血清培养基中之前没有见到的突变,这些突变显示出对SF生长条件的适应性,即病毒复制速度提高。这些突变出现在E或M蛋白质(E-107的F到L或M-60的R到C突变)中,并提示M蛋白质在病毒复制过程中的功能性意义,这在病毒于SF条件下生长的过程中变得明显(见实施例1所示的M-蛋白质的60位氨基酸R(ChimeriVaxTM-WN)。定义了在M蛋白质中的突变(ChimeriVaxTM-JE中的M60,M5)或在E蛋白质中的突变(ChimeriVaxTM-JE中的E-107,ChimeriVaxTM-DEN1和-DEN3中的E202/204,和ChimeriVaxTM-DEN2中的E251)对于疫苗的生物性质的作用。所有这些嵌合病毒都已经在临床试验中经过了检测。
材料和方法
细胞和培养基
原来自美国典型培养物保藏中心(American Type Culture Collection)得到非洲绿猴肾细胞(ATCC;Manassas,VA;CCL81;非洲绿猴肾细胞)。这些细胞适合于在SF培养基中生长,并且从Baxter(Orth,Austria)获得第133代传代物,然后通过接种到烧瓶来直接使用或在136代传代物从细胞库开始接种。在所有的实验中,非洲绿猴肾细胞的传代水平不超过第149代。在7.5%CO2、36℃的条件使细胞和病毒生长。在SF条件增殖细胞。
ChimeriVax
TM
-JE变体
通过用体外RNA转录物(储存在-80℃)电穿孔SF非洲绿猴肾细胞来启动(initiate)病毒(P1代),该体外RNA转录物与以前用于制备在临床前和临床试验中受检测的非-SF ChimeriVaxTM-JE疫苗候选物相同(Monath等,Vaccine20:1004-1018,2002),其制备如前所述(Chambers等,J.Virol.73:3095-3101, 1999)。通常在MOI为0.001pfu/细胞时作扩增传代,并在感染后3-4天收集病毒收获物(当CPE为~10%),通过慢速离心来使其澄清,补充10%的山梨醇,并储藏在-80℃。在γ-照射的FBS(HyClone;使用FBS是因为在用SF培养基制备的琼脂中细胞未形成蚀斑)存在时,通过用标准的琼脂-中性红覆盖物方法作三次连续的蚀斑纯化,随后在SF条件扩增,从而在Baxter非洲绿猴肾细胞中制备克隆的变体。用单层甲基纤维素覆盖物方法,进行测定指定标本中的病毒滴度的蚀斑试验,并在感染后第5天通过结晶紫来目测蚀斑。
ChimeriVax
TM
-DEN病毒
通过用从病毒cDNA制备的RNA转录物电穿孔非洲绿猴肾细胞,来制备ChimeriVaxTM-DEN1-4疫苗病毒。对子代病毒进行三轮蚀斑纯化来产生第7代(P7)的Pre-Master Seed(PMS)病毒。用美国现行的食品良好制造规范(Good Manufacturing Practices,cGMP)再进行三次传代,来制备成批疫苗(vaccine lot)(P10病毒)。在于非洲绿猴肾细胞中多次传代后,在嵌合体E基因中显示出一些突变(Guirakhoo等,J.Virol.78:4761-4775,2004)。这些突变中的一种(ChimeriVaxTM-DEN1中的E204)显著降低了病毒在非人的灵长类动物中的趋内脏现象(Guirakhoo等,J.Viro1.78:9998-10008,2004)。
共有测序
如上所述进行指定病毒样品的共有测序(Pugachev等,Vaccine20:996-999,2003)。简而言之,用Titan One-Tube RT-PCR试剂盒(Roche),将用TRIZOL LS试剂(Life Technologies-Gibco BRL)提取的病毒体RNA在~2-3kb长的5个重叠的cDNA扩增子中扩增。用正向的和反向的JE-和YF-特异性寡核苷酸引物的汇集物(collection)以及CEQ Dye Terminator CycleSequencing试剂盒(Beckman)来测序扩增子。用CEQ2000XL自动测序仪(Beckman Coulter)解析(resolve)测序反应产物。用Sequencher4.1.4(GeneCodes)软件比对并分析数据。当在反映正链和负链测序反应的所有色谱图(chromatogram)中观察到异质性信号时,记录核苷酸异质性。对于一些病毒,五个cDNA扩增子中只有第一个(Fragment I)经测序显示包括结构基因。
在乳鼠中的神经毒力
对小鼠的饲养和护理符合美国国立卫生研究院(National Institutes ofHealth)对实验室动物的人道使用的指南。从Taconic Farms(Germantown,NY)购买怀孕的杂交繁殖的(outbred)ICR雌鼠。养育新生小鼠并在接种前6天混合成新组。通过脑内(IC)途径给分组的8日龄乳鼠接种0.02ml的指定病毒样品。在MEM-10%FBS中对用于接种的病毒进行1:10连续稀释。反向滴定未稀释的接种物并计算每份稀释物的精确剂量。记录21天中的死亡率。YF17D对照病毒是从购买的疫苗小瓶重建的(Aventis Pasteur,Swiftwater,PA)。
在猴中的安全性和有效性试验
试验1.根据在食蟹猴中的GLP标准,研究新克隆C(M-60)ChimeriVaxTM-JE Vaccine Master Viral Bank(MVB;P11)和Production ViralBank(PVB;P12)原种与对照(YF17D疫苗病毒)相比的神经毒力/毒性分布图。将三十三只(33)首次用于实验的(experimentally-)、黄病毒-血清反应阴性的食蟹猴(用HAI试验确定)分配到表9所示的处理组。所有猴子都在第1天通过单次IC注射给予剂量,观察30天,然后安乐死并尸检。评价猴的临床指征(每日两次),和摄食(每日)、体重(每周)、和临床病理指数(pathology indices)的改变。根据国际卫生组织(World Health Organization,WHO)对黄热疫苗的要求(WHO,Technical Report Series,No.872,1998),根据临床计分系统来评临床得分。在第1天接种前和在第3、5、7、15和31天收集血样作临床病理分析(血清化学和血液学参数)。在第1天(给予剂量前(pre-dose))和第2-11天收集其它血样作定量病毒血症测定,以及在第1天(给予剂量前)和第31天收集其它血样作中和抗体滴度分析。在第31天作完整的尸检并收集组织来储藏。制备组织作肝、脾、心、肾和肾上腺的组织病理。根据Levenbook等(J.Biol.Stand.15:305,1987)所述的方法来进行脑和脊髓的组织病理,并引入WHO对黄热疫苗的要求(WHO,1998)。
试验2.进行此试验来比较在将ChimeriVaxTM-JE疫苗[原来未克隆的疫苗P5,之前它在有FBS(BB-IND#9167,Serial#000)存在时于LS5非洲绿猴肾细胞中制备,它除了在E蛋白质疏水尾部的E491的L到F改变之外不含突变]和新的克隆C(M-60突变体)ChimeriVaxTM-JE纯化的成批疫苗制品(P13)根据GLP标准单次皮下(SC)施用到食蟹猴后,在30天的时间段中的病毒血 症、免疫应答和安全性。将十八只(18)首次用于实验的、黄病毒-血清反应阴性的(通过HAI试验测定)食蟹猴如表10所示分配为处理组。给所有猴在第1天在一只臂的单个位置经SC注射给予一次剂量。评价猴的毒性临床指征(每日两次)、体重(每周)、和血清化学、血液学和凝血参数(coagulation parameter)改变。在第1天(接种前)和在第4、7、15和31天收集血样作血清化学、血液学和凝血参数分析。在第1天(接种前)和第2-11天收集其它血样作定量病毒血症分析,并在第1天(接种前)和在第31天收集其它血样作日本脑炎病毒-特异性血清抗体滴度分析。
病毒灭活的pH阈值(间接融合试验)
将黄病毒暴露于低pH(细胞膜不存在时)的结果之一是对E蛋白质的不可逆构象改变和病毒灭活(失去潜能)的诱导。在细胞膜存在时,这些构象改变是病毒膜和那些细胞膜融合所必需的,导致病毒基因组释放到宿主细胞中。蚊媒病毒如WN、DEN、YF和JE融合的pH阈值可通过用蚊细胞系C6/36的内源性融合(fusion from within,FFWI)来测量(Guirakhoo等,Virology169(1):90-99,1989)。然而,所有我们的ChimeriVaxTM病毒都显示不出任何FFWI,这可能是由于缺乏这些病毒在蚊和蚊细胞系内的充分生长所致(Johnson等,Am.J.Trop.Med.Hyg.70(1):89-97,2004)。因此,我们尝试在这里命名为“间接融合试验”的试验中,测量在暴露于不同pH水平后病毒效力的丧失。此试验间接测定了病毒膜与那些细胞膜出现融合时的pH阈值。
在pH7.0、6.8、6.6、6.4、6.2、6.0、5.8、5.6、5.4和5.0,用补充了2mML-谷氨酰胺、2.7%碳酸氢钠、10%HI FBS和1%抗生素/抗真菌溶液[(100U/ml青霉素,0.1mg/ml链霉素,0.25μg/ml Amphotericin(Sigma)]并用MES(Sigma)调节到合适pH的1X MEM进行融合。将等量分份的每种病毒以1x104蚀斑形成单位(PFU)/ml在每个pH培养基中稀释(10-1稀释度)。在暴露于每个pH值10分钟之后,将50%热灭活的(HI)FBS加到每个小瓶中并用碳酸氢钠中和每份溶液的pH。用体积100μl的每个pH值的每种病毒感染非洲绿猴肾细胞单层(以9x105细胞/孔的密度接种,在6-孔板中)来测定其滴度。进行一式双份的感染,以产生50PFU/孔;每个板保留两个未感染细胞的孔并用作阴性对照。取pH7.0和6.8的样品作为参照物。用标准蚀斑试验分析滴度。在此试验中,用病毒的连续稀释物(10-1到10-6)感染非洲绿猴肾细胞的一式双 份的孔。在感染后,用补充了2mML-谷氨酰胺、2.7%碳酸氢钠、5%HI FBS、1%抗生素/抗真菌溶液[100U/ml青霉素,0.1mg/ml链霉素,0.25μg/mlAmphotericin(Sigma)]、和44%的0.6%琼脂糖(Sigma)的1X MEM(Sigma)覆盖非洲绿猴肾单层细胞。在37℃,5%CO2温育细胞4天,然后用含1X MEM的第二层覆盖物覆盖,所述1X MEM中补充了2mM L-谷氨酰胺、2.6%碳酸氢钠、2%HI FBS、1%抗生素/抗真菌溶液、44%的0.6%琼脂糖、和3%中性红溶液(Neutral red solution)(Sigma)。在加入第二种覆盖物后24小时计数蚀斑,来测定用每毫升的蚀斑形成单位(PFU)定义的病毒滴度。
根据Vlaycheva等(J.Virol.76:6172-6184,2002)的病毒穿透入试验
为证明M-60突变(和E-107突变)利于穿透入SF非洲绿猴肾细胞,用在SF培养基中适当稀释的克隆A,C,和I病毒感染SF非洲绿猴肾细胞5,10、20或60分钟,然后用0.1M甘氨酸,0.1M NaCl,pH3.0处理3分钟来灭活胞外病毒。用PBS洗涤孔两次,然后用甲基纤维素覆盖单细胞层,之后用结晶紫在第5天染色蚀斑。穿透有效性用在甘氨酸处理后观察到的蚀斑数占用PBS而不是甘氨酸处理的对照感染孔的百分比来显示。
ChimeriVax
TM
-JE的临床试验
进行临床研究(规程H-040-003)。施用给健康成年雄性和雌性受试者的疫苗在M60(精氨酸)有天然序列。健康成年受试者/组接受了ChimeriVaxTM-JE疫苗的分级剂量的皮下剂量,包括多个对照组在内。每个剂量组试验11到33个受试者。通过在非洲绿猴肾细胞单层细胞中的蚀斑试验每日测定病毒血症。相同的实验和实验室测定了在两个实验中的病毒血症水平。
安全性评价包括对负面事件(adverse event)、体温、身体检查和实验室试验(包括病毒血症水平的测定)的记录。在大部分接受ChimeriVaxTM-JE的受试者中可见病毒血症。
在健康成年雄性和雌性受试者中进行第二项研究(规程H-040-007),其中每组31或32名受试者接受含M60半胱氨酸突变的ChimeriVaxTM-JE的分级皮下剂量(3,4,或5log10PFU)。剂量范围与在前面研究中接受了2.8,3.8,和4.8log10PFU的受试者的剂量范围类似。
结果
在未克隆的SF ChimeriVaxTM-JE病毒中的适应性突变,和克隆的变体的制品
在此研究中制备的病毒样品的图如图6所示。在SF培养物中通过用体外RNA转录本转染细胞然后作另外的扩增传代来得到原来未克隆的2代传代物(P2)样品(Pre-Master Seed候选物;PMS),所述体外RNA转录本已经用于在含FBS的培养基中制备疫苗以便用于之前的研究(Monath等,Vaccine20:1004-1018,2002)。测序此病毒的全基因组,并显示不含任何可检测的突变(表7)(注意共有测序方法不检测少数亚种群(minor subpopulation);突变的检出限为~10%)。在T25烧瓶中进行从此P2病毒到P10水平的小规模传代,来分析其在SF培养物中延长繁殖时的遗传稳定性(g.s.)(图6;g.s.传代物)。g.s.P5和g.s.P10传代物的全基因组序列在935位核苷酸有从C到T的一个核苷酸改变,导致在M-60残基从R到C的氨基酸替代(表7)。此突变在g.s.P4传代物而不是g.s.P3首先被检测为异质性。
不管小规模遗传稳定性分析的结果,当在滚瓶中从未克隆的P2PMS进行三次大规模SF传代物的制备以产生候选的未克隆主种子(Master Seed)(P3)和制备种子(Production Seed)(P4),然后在100L生物反应器中制备成批疫苗(P5)时,累积了不同的突变,以50:50%异质性观察到,由于在核苷酸1301的T到C的改变而导致了在E-107残基的F到L的氨基酸改变(表7)。这是不可接受的突变,因为它是在关键的减毒残基的从SA14-14-2序列到野生型JE序列的回复突变(Arroyo等,J.Virol.75:934-942,2001),因此可能会减弱疫苗的安全性。
基于下面提到的考虑因素,然后通过蚀斑纯化产生克隆的PMS候选物,从而稳定SF疫苗并避免不需要的突变如E-107的累积。蚀斑纯化去除了在未克隆病毒中通过体外转录引入的随机突变,该随机突变的特征为RNA合成的保真度比用YF17D特异性RNA聚合酶的病毒RNA合成的保真度低(Pugachev等,J.Virol.78:1032-1038,2004)。从未克隆的P2PMS病毒开始,通过三次连续的蚀斑纯化随后两次在SF培养基中的扩增传代得到没有发生任何氨基酸替代的处于P7的生物克隆,克隆A病毒,它被命名为非-突变体P7克隆A PMS。其基因组在6952和7147位核苷酸包含两个沉默的核苷酸改变(表7)。这些改变是可接受的,因为它们不改变病毒蛋白质的氨基酸序 列,并且位于高效病毒复制所需的顺式作用RNA元件之外。从P5g.s.病毒开始类似地制备含M-60突变的克隆C P10病毒(命名为M-60P10克隆CPMS变体)(图6)。除了需要的M-60突变之外,它只在3616位核苷酸包含沉默的核苷酸改变(表7)。另外,后来也通过单次蚀斑纯化(选择大蚀斑)和在非洲绿猴肾细胞中的一次扩增传代,从未克隆的P5成批疫苗病毒分离了研究级别的克隆I和克隆E病毒。克隆I在E-107残基含有单个氨基酸改变,这是从氨基酸F到氨基酸L的向野生型的回复突变。因此,克隆I代表了E-107回复体的纯种群。克隆E在M蛋白质的N末端包含了单个氨基酸突变,在M-5残基从Q到P的氨基酸改变。
为确认克隆的PMS变体的遗传稳定性,在SF培养物中进行相当大规模的g.s.传代物模拟制备(图6)(在T-225烧瓶中制备命名为S的连续传代物,而在5或15L的生物反应器中制备命名为F的传代物,其中非洲绿猴肾细胞生长在Cytodex I微载体珠子上)。对于两种候选物的SSS和SSF样品(分别通过三次静态(static)传代,或两次静态的和一次发酵罐(Fermenter)的传代来获得)和M-60变体的FFF样品,进行仅prM-E区(cDNA的片段I)的测序。三份g.s.样品无一在病毒的prM或E蛋白质具有任何可检测的突变,除了在克隆C的M-60突变。没有E-107突变的任何痕迹(表7)。这表明通过蚀斑纯化实现了可接受水平的遗传稳定性。随后在制备新的Master(P11)和Production Virus(P12)Seeds和最终成批疫苗(P13)的过程中确认了M-60变体的高遗传稳定性,所述新的Master(P11)和Production Virus(P12)Seeds在细胞工厂中制备,而最终成批疫苗(P13)在50L生物反应器中制备,它们都保留了M-60突变,但通过共有测序在它们的完整基因组中没有检测到其它改变。
M-60和E-107突变对于SF非洲绿猴肾细胞中的病毒生长的作用
为比较非突变体、M-60突变体、和E-107突变体病毒在SF培养物中的生长动力学,在0.001pfu/ml的MOI(通过反向滴定确认)用未克隆的P2PMS、未克隆的P5g.s.样品(M-60突变体)、或未克隆的P5成批疫苗变体(含E-107突变)、以及也包含一定比例的E-107突变的未克隆的P3主种子和P4制备种子病毒来感染细胞。收获每日等量分份的含病毒培养基,并通过蚀斑试验来滴定。如图7所示,M-60病毒生长得比非突变体P2病毒迅速,并在 感染后第3和第4天产生显著高(超过10倍)的滴度。与M-60突变相似,E-107突变也会增加病毒复制。因此,M-60和E-107突变明显赋予在SF培养物中的生长优势。为支持此结论,每日收集来自非突变体克隆A和M-60突变体克隆C病毒的S,SSS,和SSF g.s.传代物的样品(见图6),并滴定分析生长动力学,结果是M-60突变体总是产生比非突变体高最多10倍的峰值滴度(接近8log10pfu/ml)。另外,此结论通过比较克隆A,C,和I病毒在小规模SF培养物中的生长曲线得以确认,因为克隆C(M-60)和I(E-107)总是生长到比克隆A(非突变体)高的滴度。
M-60和E-107突变对于ChimeriVax
TM
-JE在乳鼠中的神经毒力的影响
已经用小鼠神经毒力试验来确保ChimeriVaxTM疫苗候选物的神经毒力不超过YF17D载体的情况。在IC接种后,YF17D疫苗对于所有年龄的小鼠都是致命的。与此相比,ChimeriVaxTM疫苗是显著减毒的。因为成熟小鼠通常对检测神经毒力的细微差异不敏感,例如由于单个氨基酸改变导致的差异,使用存活分析的更敏感乳鼠模型可以用于此目的(Guirakhoo等,Virology 257:363-372,1999;Guirakhoo等,Virology 298:146-159,2002;Monath等,J.Viro1.76:1932-1943,2002)。
给八日龄乳鼠IC接种克隆A P7病毒、克隆C P10病毒(M-60突变)、未克隆的P5成批疫苗(E-107突变)、以及之前制备的含FBS的对照ChimeriVaxTM-JE病毒(P5质控参照标准病毒(Quality Control ReferenceStandard virus);无突变)、YF17D阳性对照()的连续稀释物,或用稀释剂模拟接种。在21天阶段的死亡率、中值IC50%致死剂量值(LD50)、和死亡小鼠平均存活时间(AST)见表8所示。如预期,的神经毒力高。接种2.4log10PFU的此种病毒导致100%的死亡率和8.8天的短AST。P7非突变体和P10M-60突变体克隆与原来的含FBS型的嵌合体一样是高度减毒的,它们的LD50值>5log10PFU并且AST较长。因此,M-60突变不改变在此动物模型中疫苗的高度减毒的表型。未克隆的P5成批疫苗病毒比所述克隆的毒力明显要强,IC LD50为3.1log10PFU,但它比毒力低。后来,于GLP条件在此试验中检查克隆的M-60疫苗的制备用传代物(manufacturing passages)(主种子(Master Seed),制备种子(Production Seed), 和成批疫苗)得到类似结果。这证实了通过蚀斑纯化获得的高遗传/表型稳定性和M-60突变的用途。
在非人灵长类动物中的安全性和有效性的分析
试验1
在此试验中,用病毒作为对照,在IC施用给食蟹猴后比较克隆C(M-60突变体)、ChimeriVaxTM-JE Vaccine MasterViral Bank(MVB)和Production Viral Bank(PVB)的神经毒力(表9)。
没有观察到在摄食、体重、或血清化学方面疫苗-相关的临床指征或改变,并观察血液学参数。在1-3组的11只猴中分别有9只、4只和8只可见淋巴样增生(Lymphoid hyperplasia),其表现为脾中淋巴结的体积和数目增加。尽管此发现是在食蟹猴中常见的背景(background)发现,这些猴的组发生率高于正常,并且认为这是继发于预期的由疫苗诱导的免疫应答。值得注意在ChimeriVaxTM-JE处理组和对照参照组出现了类似的改变。[所有三组中的一些猴出现了持续时间短的低水平接种后病毒血症,这在可接受范围内,并且所有动物对用于接种的病毒发生了血清转化(seroconverted)。在第31天,在LNI试验中-处理过的猴的黄热病毒-特异性中和型抗体的滴度范围为从2.07到>6.13,并且在PRNT50试验中-处理过的猴都没有对JE病毒的交叉反应抗体。所有ChimeriVaxTM-JE MVB疫苗-处理过的猴都有≥320(从320到>20480)的JE中和抗体滴度并且在LNI试验中无对YF病毒的交叉反应抗体。所有ChimeriVaxTM-JE PVB疫苗-处理过的猴都有≥160(从160到>20480)的JE中和型抗体滴度并且无对YF病毒的交叉反应抗体。在可检测病毒血症的量级或持续时间和JE-中和型抗体滴度诱导的量级之间无可辨别的关系]。
在此试验中ChimeriVaxTM-JE MVB和PVB制品显示了最弱的神经毒力。在对于黄病毒疫苗的猴神经毒力试验中,最复杂的神经毒力测定是联合的组平均损害计分(combined group mean lesion score),它代表了平均靶面积和平均分辨区计分的均值(average of the mean target area and mean discriminatorarea scores)。食蟹猴的靶区域是黑质(substantia nigra)和脊髓的颈部和腰部膨大,并且代表了所有黄病毒都会损伤的中枢神经系统(CNS)区域。分辨区域是苍白球(globus pallidus)、壳核(putamen)、丘脑前内侧核,和丘脑外侧核, 并且代表了被具有不同毒力特性的YF17D株(和据推测的其它黄病毒)选择性损害以及区分参照株和神经毒力增加的株的CNS区域。用ChimeriVaxTM-JE MVB和PVB制品处理的猴的联合的平均损害计分显著低于参照对照组(p<0.05)。用ChimeriVaxTM-JE MVB和PVB处理的两组猴的平均分辨中心计分(mean discriminator center score)也显著低于 参照对照组的情况(p<0.05)(表9)。接受了ChimeriVaxTM-JE疫苗制品的两组猴的均值得分无统计学显著差异,并且两种制品在猴神经毒力试验中都显示了类似的低神经毒力。
因此,猴神经毒力试验的结果显示出新的(M60,克隆C)蚀斑-纯化的MVB和PVB有满意的安全性分布图。被试物品(test articles)不显示临床毒性,并且在神经病理性研究中的分辨和联合的损害计分显著低于参照对照 。通过定量病毒血症的测定,被试物品与参照对照在趋内脏现象上并无不同。
试验2
进行此试验是为了比较在食蟹猴中单次皮下(SC)施用后,原来未克隆的P5ChimeriVaxTM-JE疫苗[之前在FBS存在时在非洲绿猴肾细胞中制备,除了在E蛋白质疏水尾部的E491的L到F改变外无突变,它在生物表型方面表现为无害突变,并且在临床试验中已经对它进行了试验(Monath等,J.Infect.Dis.188:1213-1230,2003;Monath等,Vaccine20:1004-1018,2002)]和新克隆C(M-60突变体)ChimeriVaxTM-JE纯化成批疫苗(P13)的病毒血症、免疫应答和安全性。在接种了初始未克隆的P5ChimeriVaxTM-JE疫苗的5只血清反应阴性猴中,5只(100%)的血清中检测到ChimeriVaxTM-JE病毒。病毒血症的持续时间为2-5天,滴度从20到790PFU/mL。平均峰值病毒血症(±SD)为244(±310)PFU/mL,并且发生病毒血症的平均天数为3.4(±1.34)(表10)。
在接种了新的纯化的P13JE成批疫苗的4只血清反应阴性的猴子中,4只(100%)的血清中检测到ChimeriVaxTM-JE病毒。病毒血症的持续时间为2-5天,滴度从50到290PFU/mL。平均峰值病毒血症(±SD)为160(±123)PFU/mL,并且病毒血症的平均天数为3.75(±1.26)天(表10)。平均峰值病毒血症和病毒血症的天数在两个处理组中都没有显著差别(p-值分别为0.6290和0.7016;ANOVA)。
在用最初的未克隆的P5ChimeriVaxTM-JE疫苗或纯化的P13JE成批疫苗处理后,所有血清反应阴性的猴子都发生了血清转化(表10)。在第31天,在接种了未克隆的P5疫苗的5只猴中的5只(100%)的血清中,JE病毒中和型抗体的滴度的范围为从640到5120(几何均值滴度=1689)。在接种了P13ChimeriVaxTM-JE纯化的成批疫苗的4只猴中的4只(100%)的血清中,JE病毒中和型抗体的滴度的范围为从320到2560(几何均值滴度=761)。抗体滴度在处理组间无显著差别(p=0.2986,ANOVA)。
因此,将新的M-60疫苗与原来未克隆的ChimeriVaxTM-JE疫苗(除E491外无突变)在安全性(病毒血症)和免疫原性上作比较。新疫苗的趋内脏现象稍弱(理想的特征),但仍具有高度免疫原性。病毒血症和免疫原性的量级的差异是统计学上不显著的。
M-5,M-60,和E-107突变对于病毒感染性的pH阈值的影响
通过将JE病毒的SA14-14-2株的prM和E基因插入YF17D病毒骨架来制备ChimeriVaxTM-JE疫苗。SA14-14-2病毒的包膜(在ChimeriVaxTM-JE中存在)有10个氨基酸与其亲本SA14病毒不同:E107L到F、E138E到K、E176I到V、E177T到A、E227P到S、E244E到G、E264Q到H、E279K到M、E315A到V,和E439K到R的改变(Guirakhoo等,Virology257:363-372,1999)。通过定点诱变显示,这些残基中的一些参与了ChimeriVaxTM-JE病毒得到减毒作用。选择ChimeriVaxTM-JE的突变体或回复体来鉴定是否突变已经改变了这些病毒的pH阈值。为测定M-60、E-107或M-5突变是否以pH-依赖性方式影响了病毒的感染性,如材料和方法部分所述进行感染性的pH阈值的标准试验。试验了如下病毒:(1)ChimeriVaxTM-JE非突变体(在E蛋白质中含有所有10个SA14-14-2突变的克隆A,P7);(2)ChimeriVaxTM-JE E107F到L的回复体(含9个E蛋白质突变的克隆I,P6);(3)ChimeriVaxTM-JE M60R到C的突变体(含所有10个E蛋白质突变的克隆C,P10),和(4)M-5Q到P的突变体(含所有10个E蛋白质突变的克隆E,P6)(表12)。
用一系列降低的pH处理非突变体克隆A P7病毒,M-60突变体克隆CP10病毒,M-5突变体克隆E,和含E-107突变的未克隆的P5病毒,随后滴定残余的病毒感染性。三种病毒(克隆A对照病毒,克隆C M60突变体,和克隆IE-107突变体)在pH6.0后感染性开始均一地下降,并在pH5.8失 去感染性(pH阈值5.9),但M5突变体克隆E病毒除外。M-5突变体的pH阈值(pH6.3)显著高于所有其它病毒(pH5.9)(图8A)。这是M蛋白质的胞外结构域在黄病毒感染细胞的过程中起实质作用的第一个直接证据。因此,在吸收并内化病毒后由在内体中的低pH引发的融合中,M蛋白质的N-末端可能起作用,这种作用是先前认为仅由于包膜E蛋白质所致的作用。
ChimeriVaxTM-JE病毒的融合pH阈值为5.9,其低于对于其它野生型(wt)黄病毒描述的情况(Guirakhoo等,J.Gen.Virol.72:1323-1329,1991),并且可能与该病毒的减毒作用有关。
这些数据证明,ChimeriVaxTM-JE的E区中的E-107突变不改变融合的pH阈值。通常,低pH阈值意味着E-蛋白质要出现的构象改变需要特定氨基酸的更多质子化,而该构象改变是从二聚体转变为三聚体必需的。可能一或多个SA14-14-2特异性突变(除E107突变之外,该突变位于保守的融合肽内)造成保留融合的低pH阈值(pH5.9),从而保留该病毒对于所述宿主的减毒表型。M-5突变明显能够将此阈值从5.9提高到6.3,这与wt黄病毒的阈值较接近(Guirakhoo等,Virology:169(1):90-99,1989;Guirakhoo等,J.Gen.Virol.72:1323-1329,1991)。融合的pH阈值升高从理论上应该降低病毒的减毒表型,因为病毒能在较高pH融合,转变成融合活性状态所需的质子化较低。这显示是正确的,因为以1.4log10PFU通过经大脑内途径接种到3-4日龄乳鼠的M5病毒的毒力显著强于以1.7log10PFU接种的对照病毒(无M5突变的ChimeriVaxTM-JE疫苗病毒)(p=0.056)(图8B)。然而,在3-4日龄乳鼠中M5突变病毒(以1.4log10PFU的剂量)的神经毒力仍明显低于(以0.9log10PFU的剂量)(图8C),表明在疫苗病毒的包膜蛋白内的SA14-14-2突变仍提供此病毒的足够水平的减毒。
影响融合pH阈值的在其它嵌合体中的突变
用每种ChimeriVaxTM-DEN疫苗病毒的两个组进行间接融合试验:不含E蛋白质突变的ChimeriVaxTM-DEN1-4P7和在E蛋白质中含有单个突变的ChimeriVaxTM-DEN1-4P10,除ChimeriVaxTM-DEN4P10之外。将病毒与不同pH的培养基在室温温育10分钟。用标准蚀斑试验,在将pH回复到中性pH后,测定滴度。如表13所示,ChimeriVaxTM-DEN2和DEN4病毒的P7和P10的病毒灭活(融合)阈值是类似的(pH6.4)。与此相比, ChimeriVaxTM-DEN1P10的pH阈值比ChimeriVaxTM-DEN1P7病毒低0.4个单位(pH6.0与pH6.4)。对于ChimeriVaxTM-DEN3P10病毒,pH阈值的差异不太大(pH6.4与pH6.2)。
对于ChimeriVaxTM-DEN病毒的所有P7在pH6.2出现最大病毒灭活,只有ChimeriVaxTM-DEN4稍低(pH6.0)。显示出ChimeriVaxTM-DEN1P10需要显著较低的pH才能完全灭活(pH5.6)。ChimeriVaxTM-DEN1和-DEN3病毒都在E-204含有从K到R的氨基酸替代(DEN3的E-蛋白质比其它3个血清型少2个氨基酸,因此此病毒中的E-202残基与DEN1中的E-204同源)。对于DEN3嵌合体而言,融合阈值的不太大的差异可能是由于在P10病毒原种中存在WT(K)和突变的R氨基酸(E204K/R),如共同测序所显示(K:R=50:50)(Pugachev等,J.Virol.78:1032-1038,2004)。尽管存在E251突变,因为用DEN2P10嵌合体没有观察到病毒灭活的阈值有改变,可以得出结论在此残基的突变不参与病毒融合过程(图8D)。
为了确定在ChimeriVaxTM-DEN3的P10中存在K/R异质性是否会造成融合的pH阈值的不显著改变,用P7(无突变,E202K)、P10(50%突变,E202K/R)、和P15(完全突变,E202R)病毒进行间接融合测定。如图8E所示,ChimeriVaxTM-DEN3P10灭活(融合)的pH阈值是在pH6.2,介于ChimeriVaxTM-DEN3P7(pH6.4)和ChimeriVaxTM-DEN3P15(pH6.0)病毒的pH阈值之间。因为E202K到R的突变是在这些嵌合体的E-蛋白质中检测到的唯一氨基酸替代,很有可能此突变造成P15病毒融合的pH阈值发生0.4pH的偏移。
如上所述,在制备疫苗的细胞培养中出现的E204K到R的突变,会使融合的pH阈值降低0.4个pH单位。E204K到R的突变显示出产生了新的分子内H键和新的盐桥,这可能对于E二聚体的解离有显著影响。基于在存在去污剂n-辛基-B-D-葡糖苷时测定的DEN2E-蛋白质胞外结构域(S1株)的残基394的原子坐标,给ChimeriVaxTM-DEN1(PMS,P7)E蛋白质的结构建模(mode1ling)(Modis等,Proc.Natl.Acad.Sci.U.S.A.100:6986-6991,2003)。使204位的K残基变为R以模拟突变体病毒,并且重复建模来表示ChimeriVaxTM-DEN1(VL,P10)病毒的E-蛋白质结构(Guirakhoo等,J.Virol.78:9998-10008,2004)。在204位的K残基(204K)位于短环袢内,在已经显示影响神经毒力或融合的pH阈值的残基衬里(lined)的疏水袋内(Lee等, Virology 232:281-290,1997;Lindenbach等,2001 Flaviviridae:the viruses andtheir replication.Fields Virology,eds.Knipe D.M.,and Howley PM.[LippincottWilliams and Wilkins,Philadelphia],1,991-1004;Monath等,J.Virol.76:1932-1943,2002)。在图8F中比较了疫苗病毒(204R)与PMS(204K)病毒的E-同型二聚体结构的同源性模型。E单体之一的204K和261H的侧链显示出分别与在相对单体上的252V和253L残基的骨架原子形成H键。在204位,预测在疫苗病毒(VL P10)的E蛋白质中的R会自身再定向(reorient),从而会失去这些氢(H)键。然而,突变体R的侧链与261H和257E邻近,导致在204R和261H之间产生新的分子内H键,并可能在204R和257E之间产生新的盐桥。因为组氨酸的pk可能是大约6.0,稍微低于融合阈值(pH~6.4),Guirakhoo等,(J.Virol.78:9998-10008,2004)原来假设在204R和261H之间预测的新H键以及在204R和257E之间的盐桥可能会影响融合的pH阈值。此理论显示出是正确的,因为本文描述的试验显示ChimeriVaxTM-DEN1的融合阈值是大约6.0,比它的P7病毒(pH6.4)低0.4个pH单位。在残基204通过R引入的新的分子间键明显加强了E-二聚体的连接(association),因此转变成低pH需要合适残基(例如H 261)的更多质子化。较低的融合阈值影响了病毒在猴子中的趋内脏现象并降低了对通过i.c.途径接种的乳鼠的神经毒力(Guirakhoo等,J.Virol.78:9998-10008,2004)。
在ChimeriVaxTM-DEN3P10疫苗的E-蛋白质中的E202K到R的替代与ChimeriVaxTM-DEN1P10疫苗中的E204突变同源。与ChimeriVaxTM-DEN1P10一样,ChimeriVaxTM-DEN3P10(在含K和R残基的残基202上是异质的)显示了比P7低的融合pH阈值(~0.2pH单位)。当突变固定在ChimeriVaxTM-DEN3的P15时,融合的pH阈值进一步下降(0.4个pH单位,类似于ChimeriVaxTM-DEN1P10)。此数据显示残基202/204可以是所有登革病毒中减毒的通用决定簇。现在,ChimeriVaxTM-DEN3和-DEN4的P10疫苗病毒不含有此突变,而且这两种病毒在接种了四价疫苗配制剂的猴子中都诱导了较高的病毒血症水平(Guirakhoo等,J.Virol.78:4761-4775,2004)。仍需观察在ChimeriVaxTM-DEN3或ChimeriVaxTM-DEN4中的K到R突变是否会降低它们在它们的宿主中的趋内脏现象。
之前有报道WT-JE融合的pH阈值为6.4(Guirakhoo等,J.Gen.Virol.72:1323-1329,1991)。本研究中,ChimeriVaxTM-JE的所有变体的pH阈值都 是5.9。在这些实验中观察到的低pH阈值可能是由于在ChimeriVaxTM-JE包膜蛋白中存在10种减毒突变中的一或多个所致。此突变可能会加强E-蛋白质二聚体的连接,从而解离和转变为三聚体结构以及随后的融合需要较低的pH。这里的数据显示E107F到L的突变(位于E-蛋白质的结构域II的cd-环袢内)和E279M到K的突变(位于结构域II的疏水袋内)都不会造成pH阈值下降。可能在JE E蛋白质中的其它突变会影响融合的pH阈值。对十分类似JE E蛋白质的TBE病毒E蛋白质的晶体结构的分析,可有助于预测一经改变会改变融合的pH阈值的残基。基于此模型,可能是在残基E244G和/或E264H中的突变导致ChimeriVaxTM-JE病毒融合的pH阈值比WT JE低。
M-60和E-107突变对病毒穿透有效性的影响
用Chambers的方法(Vlaycheva等,J.Virol.76:6172-6184,2002)来检测M-60(克隆C病毒)和E-107(克隆I病毒)突变对病毒穿透SF非洲绿猴肾细胞的影响。在此试验中,用适当稀释的病毒(达到在每个时间点产生~50个蚀斑/孔)感染SF非洲绿猴肾细胞5,10,20,或60分钟。通过加入酸性甘氨酸溶液(silution)来灭活未内化的病毒,而用PBS(中性pH)处理对照的平行孔。用PBS洗涤细胞并用甲基纤维素覆盖物覆盖,随后在第5天目测并计数蚀斑。穿透的有效性表示为在甘氨酸-处理过的孔中的蚀斑平均数占对照(PBS处理过的孔)中蚀斑数的百分比。初步穿透试验结果如图9A所示。重要的是在5和10分钟的时间点,此时更可能检测到突变对穿透的作用,发生穿透的克隆C和克隆I病毒的百分比非突变体克隆A病毒高。结果不是如标准偏差条所显示的那样是统计学显著性的,并且需要在其它重复试验中予以确证。然而,此实验提示M-60和E-107突变都可以提高ChimeriVaxTM-JE病毒与在SF条件生长的细胞的膜融合有效性。M-60和E-107残基对膜融合过程起作用的可能机制见图9B所示。M-60残基位于病毒膜中,而E-107残基插入细胞膜中,并且在E蛋白质发生低pH-依赖性重排后这两个膜被迫融合(根据我们的数据,M蛋白质的胞外结构域可对其予以促进)。在这两个残基中任一个的更合适的氨基酸可以促进膜的融合。
因为我们的数据首次发现M蛋白质的胞外结构域及其跨膜结构域都有功能性意义,现在认为完整的M蛋白质是为了开发新的活减毒疫苗而作诱变来使黄病毒减毒的有吸引力的靶标。例如,可以将随机或特定的(在蛋白 质结构的进一步分析后)氨基酸改变、或长度渐增的例如1,2,3,4,5个氨基酸等等的缺失掺入整个蛋白质中,期待病毒的生物表型会改变,从而造成明显减毒。
临床试验的结果
表11A和B中比较了从上述临床试验获得的具有精氨酸和半胱氨酸M60残基的ChimeriVaxTM-JE的病毒血症图谱。与29-50%接受了ChimeriVaxTM-JE M60半胱氨酸的受试者相比,在接受了ChimeriVaxTM-JEM60精氨酸的受试者中,67-100%的受试者在一或多天是有病毒血症的。接受了ChimeriVaxTM-JE M60精氨酸的受试者的平均最高病毒血症水平在13到40PFU/ml的范围,而在ChimeriVaxTM-JE M60半胱氨酸的情况下,平均最高病毒血症水平为3.5-6.3PFU/ml。在ChimeriVaxTM-JE M60精氨酸的情况下,病毒血症的持续时间明显更长。
这些数据证明,在含M60突变的疫苗的情况下,病毒血症的水平明显较低。病毒血症是疫苗病毒的趋内脏现象(毒力)的指标。伴有低病毒血症的疫苗被认为是较安全的,因为支持病毒复制并造成病毒血症的器官的细胞损害和功能障碍下降,病毒会穿过血脑屏障并侵入中枢神经系统的可能性也下降。在其它试验中,显示M60突变体和非突变体一样对人类是高免疫原性的。
表1.小蚀斑(P10PMS)(P/N IT-0116;L/N I020504A)(从p5Run1疫苗批(lot)纯化的蚀斑)的共有序列。
位置 | 氨基酸改变 | NT位置 | NT改变 |
M(66) | 亮氨酸→脯氨酸 | 954 | CTA→CCA |
E(313) | 甘氨酸→精氨酸 | 1919 | GGG→AGG |
| 天冬酰胺(沉默) | 2926 | AAC→AAT |
| 甘氨酸(沉默) | 7126 | GGA→GGG |
表2.大蚀斑PMS(P10,PMS)(P/N IT-0117;L/N I030804A)(源自p5Run1疫苗批)的共有序列。
位置 | 氨基酸改变 | NT位置 | NT改变 |
E(313) | 甘氨酸→精氨酸 | 1919 | GGG→AGG |
表3.在无血清条件下在非洲绿猴肾细胞中对S蚀斑PMS(p10)作另外2次传代后分离的大蚀斑的序列。
表4.对于HepG2观察到的CPE。
感染后天数 | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
WN01 | 0% | 0% | 0% | 0% | 0% | 30% | 90% | ~100% | 100% |
WN02P5 | 0% | 0% | 0% | 5% | 30% | 50% | ~100% | 100% | |
WNL | 0% | 0% | 0% | 0% | 0% | 30% | 90% | ~100% | 100% |
WNS | 0% | 0% | 0% | 5% | 30% | 50% | ~100% | 100% | |
YF/17D | 0% | 0% | 0% | 20% | 50% | 70% | ~100% | 100% | |
表5.接种了ChimeriVaxTM-WN02疫苗或的猴的病毒血症。
*病毒血症用pfu/mL表示
**第1天:研究的第1天,猴在研究第1天接种
0PFU/mL表示在检出限以下,理论的试验截留值(assay cutoff)=10PFU/mL
表6.从蚀斑分离物直接获得的YF-WN嵌合体的M区序列,所述蚀斑分离物来自用WN02疫苗病毒接种的有病毒血症的猴。
猴# | 病毒血症天数 | 蚀斑分离物# | 可见蚀斑形态(挑取时) | M66存在? | 其它M突变 |
21205 | 4 | #4 | SP | 否 | 无 |
2808 | 3 | #8 | SP | 否 | 无 |
2808 | 3 | #9 | LP | 否 | M60(R到G) |
21191 | 2 | #10 | LP | 否 | 无 |
21191 | 1 | #14 | SP | 否 | M61(V到A) |
21191 | 1 | #15 | SP | 否 | 无 |
21191 | 1 | #16 | LP | 否 | M63(F到S) |
表7.未克隆的和克隆的SF ChimeriVaxTM-JE样品(见表6)的核苷酸和氨基酸序列。
a:从基因组的开始起b:从指定蛋白质N-末端起
表8.克隆A P7、克隆C P10、未克隆的P5、含FBS的标准品、和病毒在8日龄乳鼠中的神经毒力。
表9.M-60(克隆C)主种子及制备种子与对照相比对食蟹猴的神经毒力。
1PFU=蚀斑-形成单位
2第1、2和3组中分别有11只中的4只、11只中的2只和11只中的1只动物被排除在分数的计算之外,这是因为在第1天(接种前)在回顾性PRNT50试验中发现它们是JE-血清阳性的,PRNT50试验比用于预筛选的HAI试验更灵敏。
表10.食蟹猴的病毒血症和免疫原性的量级的比较,所述食蟹猴SC接种了在含FBS培养基中产生的原本未克隆的P5ChimeriVaxTM-JE疫苗(除E491外不含突变)和新的克隆C P13纯化的成批疫苗(M-60突变体)。
1第1、2和3组中分别有6只中的2只、6只中的1只、6只中的2只动物被排除在值的计算之外,这是因为在第1天(接种前)在回顾性PRNT50试验中发现它们是JE-血清阳性的,PRNT50试验比用于预筛选的HAI试验更灵敏。
表11A.参与了试验H-040-003的受试者的病毒血症图谱,其中给所述受试者施用了带有M60精氨酸的ChimeriVaxTM-JE。黑体的剂量范围与另一试验(H-040-007)中给予的类似,在所述另一试验中施用了突变体M-60半胱氨酸疫苗。
表11B.参与了试验H-040-007的受试者的病毒血症图谱,其中给所述受试者施用了带有M60半胱氨酸的ChimeriVaxTM-JE。
表12.通过每种ChimeriVaxTM-JE疫苗的融合试验发现的融合pH阈值
表13.通过每对ChimeriVaxTM-DEN P7和P10的间接融合试验发现的融合pH阈值
病毒 | 用于融合的pH阈值 |
ChimeriVaxTM-DEN1PMS P7 | 6.4 |
ChimeriVaxTM-DEN1VL P10 | 6.0 |
ChimeriVaxTM-DEN2PMS P7 | 6.4 |
ChimeriVaxTM-DEN2VL P10 | 6.4 |
ChimeriVaxTM-DEN3PMS P7 | 6.4 |
ChimeriVaxTM-DEN3VL P10 | 6.2 |
ChimeriVaxTM-DEN4PMS P7 | 6.4 |
ChimeriVaxTM-DEN4VL P10 | 6.4 |
表14
YF/黄病毒嵌合物的改造
病毒 嵌合的C/prM接合1 嵌合的E/NS1接合2 5′ 3′ 去除或(产
连接3 连接4 生)的位点5
YF/WN X-cactgggagagcttgaaggtc aaagccagttgcagccgcggtttaa AatII NsiI
(SEQ ID NO:1) (SEQ ID NO:2)
YF/DEN-1 X-aaggtagactggtgggctccc gatcctcagtaccaaccgcggtttaa AatII SphI DEN中的SphI
(SEQ ID NO:3) (SEQ ID NO:4)
YF/DEN-2 X-aaggtagattggtgtgcattg aaccctcagtaccacccgcggtttaa AatII SphI
(SEQ ID NO:5) (SEQ ID NO:6)
YF/DEN-3 X-aaggtgaattgaagtgctcta acccccagcaccacccgcggtttaa AatII SphI DEN中XhoI
(SEQ ID NO:7) (SEQ ID NO:8) (DEN中的SphI)
YF/DEN-4 X-aaaaggaacagttgttctcta acccgaagtgtcaaccgcggtttaa AatII NsiI
(SEQ ID NO:9) (SEQ ID NO:10)
YF/SLE X-aacgtgaatagttggatagtc accgttggtcgcacccgcggtttaa AatII SphI SLE中的AatII
(SEQ ID NO:11) (SEQ ID NO:12)
YF/MVE X-aatttcgaaaggtggaaggtc gaccggtgtttacagccgcggtttaa AatII AgeI (Y中的AgeI)
(SEQ ID NO:13) (SEQ ID NO:14)
YF/TBE X-tactgcgaacgacgttgccac actgggaacctcacccgcggtttaa AatII AgeI (YF中的AgeI)
(SEQ ID NO:15) (SEQ ID NO:16)
1,2:这两列显示了用于产生与C/prM或E/NS1接合对应的嵌合YF/黄病毒引物的寡核苷酸。(见正文)。X=YF衣壳的羧基末端编码序列。下划线区对应于紧邻NarI位点(反义-ccgcgg)上游的靶向异源序列。此位点允许将PCR产物插入产生全长cDNA模板所需的Yfm5.2(NarI)质粒。其它核苷酸对于异源病毒是特异的。寡核苷酸引物按5′到3′方向列举。
3,4:列举了用于产生限制片段的独特限制位点,所述限制片段可以被分离并体外连接以产生全长的嵌合cDNA模板。因为一些序列不包含方便的位点,有时需要对合适位点作改造(注脚5)。
5:括号中是必须在YF骨架或异源病毒中产生的限制性酶位点,从而允许高效的体外连接。必须去除不在括号中的位点。通过对各个克隆作cDNA沉默诱变来进行所有这些修饰。空白间隔表明不需要对cDNA克隆作修饰。
ChimerivaxWN02 改为装瓶终产物(Run1)L/N# 02H01; P/N#FP-0008
[链]
SEQ ID NOS:20与21
1 NGTAAATCCT GTGTGCTAAT TGAGGTGCAT TGGTCTGCAA
41 ATCGAGTTGC TAGGCAATAA ACACATTTGG ATTAATTTTA
81 ATCGTTCGTT GAGCGATTAG CAGAGAACTG ACCAGAACAT
M
121 GTCTGGTCGT AAAGCTCAGG GAAAAACCCT GGGCGTCAAT
S G R K A Q G K T L G V N
161 ATGGTACGAC GAGGAGTTCG CTCCTTGTCA AACAAAATAA
M V R R G V R S L S N K I
201 AACAAAAAAC AAAACAATT GGAAACAGAC CTGGACCTTC
K Q K T K Q I G N R P G P S
241 AAGAGGTGTT CAAGGATTTA TCTTTTTCTT TTTGTTCAAC
R G V Q G F I F F F L F N
281 ATTTTGACTG GAAAAAAGAT CACAGCCCAC CTAAAGAGGT
I L T G K K I T A H L K R
321 TGTGGAAAAT GCTGGACCCA AGACAAGGCT TGGCTGTTCT
L W K M L D P R Q G L A V L
361 AAGGAAAGTC AAGAGAGTGG TGGCCAGTTT GATGAGAGGA
R K V K R V V A S L M R G
401 TTGTCCTCAA GGAAACGCCG TTCCCATGAT GTTCTGACTG
L S S R K R R S H D V L T
441 TGCAATTCCT AATTTTGGGA ATGCTGTTGA TGACGGGTGG
V Q F L I L G M L L M T G G
481 AGTTACCCTC TCTAACTTCC AAGGGAAGGT GATGATGACG
V T L S N F Q G K V M M T
521 GTAAATGCTA CTGACGTCAC AGATGTCATC ACGATTCCAA
V N A T D V T D V I T I P
561 CAGCTGCTGG AAAGAACCTA TGCATTGTCA GAGCAATGGA
T A A G K N L C I V R A M D
601 TGTGGGATAC ATGTGCGATG ATACTATCAC TTATGAATGC
V G Y M C D D T I T Y E C
641 CCAGTGCTGT CGGCTGGTAA TGATCCAGAA GACATCGACT
P V L S A G N D P E D I D
ChimerivaxWN02 改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
681 GTTGGTGCAC AAAGTCAGCA GTCTACGTCA GGTATGGAAG
C W C T K S A V Y V R Y G R
721 ATGCACCAAG ACACGCCACT CAAGACGCAG TCGGAGGTCA
C T K T R H S R R S R R S
761 CTGACAGTGC AGACACACGG AGAAAGCACT CTAGCGAACA
L T V Q T H G E S T L A N
801 AGAAGGGGGC TTGGATGGAC AGCACCAAGG CCACAAGGTA
K K G A W M D S T K A T R Y
841 TTTGGTAAAA ACAGAATCAT GGATCTTGAG GAACCCTGGA
L V K T E S W I L R N P G
881 TATGCCCTGG TGGCAGCCGT CATTGGTTGG ATGCTTGGGA
Y A L V A A V I G W M L G
921 GCAACACCAT GCAGAGAGTT GTGTTTGTCG TGCTATTGCT
S N T M Q R V V F V V L L L
961 TTTGGTGGCC CCAGCTTACA GCTTCAACTG CCTTGGAATG
L V A P A Y S F N C L G M
1001 AGCAACAGAG ACTTCTTGGA AGGAGTGTCT GGAGCAACAT
S N R D F L E G V S G A T
1041 GGGTGGATTT GGTTCTCGAA GGCGACAGCT GCGTGACTAT
W V D L V L E G D S C V T I
1081 CATGTCTAAG GACAAGCCTA CCATCGACGT CAAGATGATG
M S K D K P T I D V K M M
1121 AATATGGAGG CGGCCAACCT GGCAGAGGTC CGCAGTTATT
N M E A A N L A E V R S Y
1161 GCTATTTGGC TACCGTCAGC GATCTCTCCA CCAAAGCTGC
C Y L A T V S D L S T K A A
1201 ATGCCCGACC ATGGGAGAAG CTCACAATGA CAAACGTGCT
C P T M G E A H N D K R A
1241 GACCCAGCTT TTGTGTGCAG ACAAGGAGTG GTGGACAGGG
D P A F V C R Q G V V D R
1281 GCTGGGGCAA CGGCTGCGGA TTTTTTGGCA AAGGATCCAT
G W G N G C G F F G K G S I
1321 TGACACATGC GCCAAATTTG CCTGCTCTAC CAAGGCAATA
D T C A K F A C S T K A I
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
1361 GGAAGAACCA TCTTGAAAGA GAATATCAAG TACGAAGTGG
G R T I L K E N I K Y E V
1401 CCATTTTTGT CCATGGACCA ACTACTGTGG AGTCGCACGG
A I F V H G P T T V E S H G
1441 AAATTACTCC ACACAGGTTG GAGCCACTCA GGCCGGCCGA
N Y S T Q V G A T Q A G R
1481 TTCAGCATCA CTCCTGCTGC GCCTTCATAC ACACTAAAGC
F S I T P A A P S Y T L K
1521 TTGGAGAATA TGGAGAGGTG ACAGTGGACT GTGAACCACG
L G E Y G E V T V D C E P R
1561 GTCAGGGATT GACACCAATG CATACTACGT GATGACTGTT
S G I D T N A Y Y V M T V
1601 GGAACAAAGA CGTTCTTGGT CCATCGTGAG TGGTTCATGG
G T K T F L V H R E W F M
1641 ACCTCAACCT CCCTTGGAGC AGTGCTGGAA GTACTGTGTG
D L N L P W S S A G S T V W
1681 GAGGAACAGA GAGACGTTAA TGGAGTTTGA GGAACCACAC
R N R E T L M E F E E P H
1721 GCCACGAAGC AGTCTGTGAT AGCATTGGGC TCACAAGAGG
A T K Q S V I A L G S Q E
1761 GAGCTCTGCA TCAAGCTTTG GCTGGAGCCA TTCCTGTGGA
G A L H Q A L A G A I P V E
1801 ATTTTCAAGC AACACTGTCA AGTTGACGTC GGGTCATTTG
F S S N T V K L T S G H L
1841 AAGTGTAGAG TGAAGATGGA AAAATTGCAG TTGAAGGGAA
K C R V K M E K L Q L K G
1881 CAACCTATGG CGTCTGTTCA AAGGCTTTCA AGTTTCTTAG
T T Y G V C S K A F K F L R
1921 GACTCCCGTG GACACCGGTC ACGGCACTGT GGTGTTGGAA
T P V D T G H G T V V L E
1961 TTGCAGTACA CTGGCACGGA TGGACCTTGC AAAGTTCCTA
L Q Y T G T D G P C K V P
2001 TCTCGTCAGT GGCTTCATTG AACGACCTAA CGCCAGTGGG
I S S V A S L N D L T P V G
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
2041 CAGATTGGTC ACTGTCAACC CTTTTGTTTC AGTGGCCACG
R L V T V N P F V S V A T
2081 GCCAACGCTA AGGTCCTGAT TGAATTGGAA CCACCCTTTG
A N A K V L I E L E P P F
2121 GAGACTCATA CATAGTGGTG GGCAGAGGAG AACAACAGAT
G D S Y I V V G R G E Q Q I
2161 CAATCACCAT TGGCACAAGT CTGGAAGCAG CATTGGCAAA
N H H W H K S G S S I G K
2201 GCCTTTACAA CCACCCTCAA AGGAGCGCAG AGACTAGCCG
A F T T T L K G A Q R L A
2241 CTCTAGGAGACACAGCTTGG GACTTTGGGAT CAGTTGGAGG
A L G D T A W D F G S V G G
2281 GGTGTTCACTAGTGTTCGGC GGGCTGTCCA TCAAGTGTTC
V F T S V G R A V H Q V F
2321 GGAGGAGCAT TCCGCTCACT GTTCGGAGGC ATGTCCTGGA
G G A F R S L F G G M S W
2361 TAACGCAAGG ATTGCTGGGG GCTCTCCTGT TGTGGATGG
I T Q G L L G A L L L W M G
2401 CATCAATGCT CGTGATAGGT CCATAGCTCT CACGTTTCTC
I N A R D R S I A L T F L
2441 GCAGTTGGAG GAGTTCTGCT CTTCCTCTDC GTGAACGTGG
A V G G V L L F L S V N V
2481 GCGCCGATCA AGGATGCGCC ATCAACTTTG GCAAGAGAGA
G A D Q G C A I N F G K R E
2521 GCTCAAGTGC GGAGATGGTA TCTTCATATT TAGAGACTCT
L K C G D G I F I F R D S
2561 GATGACTGCC TGAACAAGTA CTCATACTAT CCAGAAGATC
D D W L N K Y S Y Y P E D
2601 CTGTGAAGCT TGCATCAATA GTGAAAGCCT CTTTTGAAGA
P V K L A S I V K A S F E E
2641 AGGGAAGTGT GGCCTAAATT CAGTTGACTC CCTTGAGCAT
G K C G L N S V D S L E H
2681 GAGATGTGGA GAAGCAGGGC AGATGAGATC AATGCCATTT
E M W R S R A D E I N A I
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
2721 TTGAGGAAAA CGAGGTGGAC ATTTCTGTTG TCGTGCAGGA
F E E N E V D I S V V V Q D
2761 TCCAAAGAAT GTTTACCAGA GAGGAACTCA TCCATTTTCC
P K N V Y Q R G T H P F S
2801 AGAATTCGGG ATGGTCTGCA GTATGGTTGG AAGACTTGGG
R I R D G L Q Y G W K T W
2841 GTAAGAACCT TGTGTTCTCC CCAGGGAGGA AGAATGGAAG
G K N L V F S P G R K N G S
2881 CTTCATCATA GATGGAAAGT CCAGGAAAGA ATGCCCGTTT
F I I D G I S R K E C P F
2921 TCAAACCGGG TCTGGAATTC TTTCCAGATA GAGGAGTTTG
S N R V W N S F Q I E E F
2961 GGACGGGAGT GTTCACCACA CGCGTGTACA TGGACGCAGT
G T G V F T T R V Y M D A V
3001 CTTTGAATAC ACCATAGACT GCGATGGATC TATCTTGGGT
F E Y T I D C D G S I L G
3041 GCAGCGGTGA ACGGAAAAAA GAGTGCCCAT GGCTCTCCAA
A A V N G K K S A H G S P
3081 CATTTTGGAT GGGAAGTCAT GAAGTAAATG GGACATGGAT
T F W M G S H E V N G T W M
3121 GATCCACACC TTGGAGGCAT TAGATTACAA GGAGTGTGAG
I H T L E A L D Y K E C E
3161 TGGCCACTGA CACATACGAT TGGAACATCA GTTGAAGAGA
W P L T H T I G T S V E E
3201 GTGAAATGTT CATGCCGAGA TCAATCGGAG GCCCAGTTAG
S E M F M P R S I G G P V S
3241 CTCTCACAAT CATATCCCTG GATACAAGGT TCAGACGAAC
S H N H I P G Y K V Q T N
3281 GGACCTTGGA TGCAGGTACC ACTAGAAGTG AAGAGAGAAG
G P W M Q V P L E V K R E
3321 CTTGCCCAGG GACTAGCGTG ATCATTGATG GCAACTGTGA
A C P G T S V I I D G N C D
3361 TGGACGGGGA AAATCAACCA GATCCACCAC GGATAGCGGG
G R G K S T R S T T D S G
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
3401 AAAGTTATTC CTGAATGGTG TTGCCGCTCC TGCACAATGC
K V I P E W C C R S C T M
3441 CGCCTGTGAG CTTCCATGGT AGTGATGGGT GTTGGTATCC
P P V S F H G S D G C W Y P
3481 CATGGAAATT AGGCCAAGGA AAACGCATGA AAGCCATCTG
M E I R P R K T H E S H L
3521 GTGCGCTCCT GGGTTACAGC TGGAGAAATA CATGCTGTCC
V R S W V T A G E I H A V
3561 CTTTTGGTTT GGTGAGCATG ATGATAGCAA TGGAAGTGGT
P F G L V S M M I A M E V V
3601 CCTAAGGAAA AGACAGGGAC CAAAGCAAAT GTTGGTTGGA
L R K R Q G P K Q M L V G
3641 GGAGTAGTGC TCTTGGGAGC AATGCTGGTC GGGCAAGTAA
G V V L L G A M L V G Q V
3681 CTCTCCTTGA TTTGCTGAAA CTCACAGTGG CTGTGGGATT
T L L D L L K L T V A V G L
3721 GCATTTCCAT GAGATGAACA ATGGAGGAGA CGCCATGTAT
H F H E M N N G G D A M Y
3761 ATGGCGTTGA TTGCTGCCTT TTCAATCAGA CCAGGGCTGC
M A L I A A F S I R P G L
3801 TCATCGGCTT TGGGCTCAGG ACCCTATGGA GCCCTCGGGA
L I G F G L R T L W S P R E
3841 ACGCCTTGTG CTGACCCTAG GAGCAGCCAT GGTGGAGATT
R L V L T L G A A M V E I
3881 GCCTTGGGTG GCGTGATGGG CGGCCTGTGG AAGTATCTAA
A L G G V M G G L W K Y L
3921 ATGCAGTTTC TCTCTGCATC CTGACAATAA ATGCTGTTGC
N A V S L C I L T I N A V A
3961 TTCTAGGAAA GCATCAAATA CCATCTTGCC CCTCATGGCT
S R K A S N T I L P L M A
4001 CTGTTGACAC CTGTCACTAT GGCTGAGGTG AGACTTGCCG
L L T P V T M A E V R L A
4041 CAATGTTCTT TTGTGCCATG GTTATCATAG GGGTCCTTCA
A M F F C A M V I I G V L H
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
4081 CCAGAATTTC AAGGACACCT CCATGCAGAA GACTATACCT
Q N F K D T S M Q K T I P
4121 CTGGTGGCCC TCACACTCAC ATCTTACCTG GGCTTGACAC
L V A L T L T S Y L G L T
4161 AACCTTTTTT GGGCCTGTGT GCATTTCTGG CAACCCGCAT
Q P F L G L C A F L A T R I
4201 ATTTGGGCGA AGGAGTATCC CAGTGAATGA GGCACTCGCA
F G R R S I P V N E A L A
4241 GCAGCTGGTC TAGTGGGAGT GCTGGCAGGA CTGGCTTTTC
A A G L V G V L A G L A F
4281 AGGAGATGGA GAACTTCCTT GGTCCGATTG CAGTTGGAGG
Q E M E N F L G P I A V G G
4321 ACTCCTGATG ATGCTGGTTA GCGTGGCTGG GAGGGTGGAT
L L M M L V S V A G R V D
4361 GGGCTAGAGC TCAAGAAGCT TGGTGAAGTT TCATGGGAAG
G L E L K K L G E V S W E
4401 AGGAGGCGGA GATCAGCGGG AGTTCCGCCC GCTATGATGT
E E A E I S G S S A R Y D V
4441 GGCACTCAGT GAACAAGGGG AGTTCAAGCT GCTTTCTGAA
A L S E Q G E F K L L S E
4481 GAGAAAGTGC CATGGGACCA GGTTGTGATG ACCTCGCTGG
E K V P W D Q V V M T S L
4521 CCTTGGTTGG GGCTGCCCTC CATCCATTTG CTCTTCTGCT
A L V G A A L H P F A L L L
4561 GGTCCTTGCT GGGTGGCTGT TTCATGTCAG GGGAGCTAGG
V L A G W L F H V R G A R
4601 AGAAGTGGGG ATGTCTTGTG GGATATTCCC ACTCCTAAGA
R S G D V L W D I P T P K
4641 TCATCGAGGA ATGTGAACAT CTGGAGGATG GGATTTATGG
I I E E C E H L E D G I Y G
4681 CATATTCCAG TCAACCTTCT TGGGGGCCTC CCAGCGAGGA
I F Q S T F L G A S Q R G
4721 GTGGGAGTGG CACAGGGAGG GGTGTTCCAC ACAATGTGGC
V G V A Q G G V F H T M W
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
4761 ATGTCACAAG AGGAGCTTTC CTTGTCAGGA ATGGCAAGAA
H V T R G A F L V R N G K K
4801 GTTGATTCCA TCTTGGGCTT CAGTAAAGGA AGACCTTGTC
L I P S W A S V K E D L V
4841 GCCTATGGTG GCTCATGGAA GTTGGAAGGC AGATGGGATG
A Y G G S W K L E G R W D
4881 GAGAGGAAGA GGTCCAGTTG ATCGCGGCTG TTCCAGGAAA
G E E E V Q L I A A V P G K
4921 GAACGTGGTC AACGTCCAGA CAAAACCGAG CTTGTTCAAA
N V V N V Q T K P S L F K
4961 GTGAGGAATG GGGGAGAAAT CGGGGCTGTC GCTCTTGACT
V R N G G E I G A V A L D
5001 ATCCGAGTGG CACTTCAGGA TCTCCTATTG TTAACAGGAA
Y P S G T S G S P I V N R N
5041 CGGAGAGGGTG ATTGGGCTGT ACGGCAATGG CATCCTTGTC
G E V I G L Y G N G I L V
5081 GGTGACAACT CCTTCGTGTC CGCCATATCC CAGACTGAGG
G D N S F V S A I S Q T E
5121 TGAAGGAAGA AGGAAAGGAG GAGCTCCAAG AGATCCCGAC
V K E E G K E E L Q E I P T
5161 AATGCTAAAG AAAGGAATGA CAACTGTCCT TGATTTTCAT
M L K K G M T T V L D F H
5201 CCTGGAGCTG GGAAGACAAG ACGTTTCCTC CCACAGATCT
P G A G K T R R F L P Q I
5241 TGGCCGAGTG CGCACGGAGA CGCTTGCGCA CTCTTGTGTT
L A E C A R R R L R T L V L
5281 GGCCCCCACC AGGGTTGTTC TTTCTGAAAT GAAGGAGGCT
A P T R V V L S E M K E A
5321 TTTCACGGCC TGGACGTGAA ATTCCACACA CAGGCTTTTT
F H G L D V K F H T Q A F
5361 CCGCTCACGG CAGCGGGAGA GAAGTCATTG ATGCCATGTG
S A H G S G R E V I D A M C
5401 CCATGCCACC CTAACTTACA GGATGTTGGA ACCAACTAGG
H A T L T Y R M L E P T R
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
5441 GTTGTTAACT GGGAAGTGAT CATTATGGAT GAAGCCCATT
V V N W E V I I M D E A H
5481 TTTTGGATCC AGCCAGCATA GCCGCTAGAG GTTGGGCAGC
F L D P A S I A A R G W A A
5521 GCACAGAGCT AGGGCAAATG AAAGTGCAAC AATCTTGATG
H R A R A N E S A T I L M
5561 ACAGCCACAC CGCCTGGGAC TAGTGATGAA TTTCCACATT
T A T P P G T S D E F P H
5601 CAAATGGTGA AATAGAAGAT GTTCAAACGG ACATACCCAG
S N G E I E D V Q T D I P S
5641 TGAGCCCTGG AACACAGGGC ATGACTGGAT CCTGGCTGAC
E P W N T G H D W I L A D
5681 AAAAGGCCCA CGGCATGGTT CCTTCCATCC ATCAGAGCIG
K R P T A W F L P S I R A
5721 CAAATGTCAT GGCTGCCTCT TTGCGTAAGG CTGGAAAGAG
A N V M A A S L R K A G K S
5761 TGTGGTGGTC CTGAACAGGA AAACCTTTGA GAGAGAATAC
V V V L N R K T F E R E Y
5801 CCCACGATAA AGCAGAAGAA ACCTGAGTTT ATATTGGCCA
P T I K Q K K P D F I L A
5841 CTGACATAGC TGAAATGGGA GCCAACCTTT GCGTGGAGCG
T D I A E M G A N L C V E R
5881 AGTGCTGGAT TGCAGGACGG CTTTTAAGCC TGTGCTTGTG
V L D C R T A F K P V L V
5921 GATGAAGGGA GGAAGGTGGC AATAAAAGGG CCACTTCGTA
D E G R K V A I K G P L R
5961 TCTCCGCATC CTCTGCTGCT CAAAGGAGGG GGCGCATTGG
I S A S S A A Q R R G R I G
6001 GAGAAATCCC AACAGAGATG GAGACTCATA CTACTATTCT
R N P N R D G D S Y Y Y S
6041 GAGCCTACAA GTGAAAATAA TGCCCACCAC GTCTGCTGGT
E P T S E N N A H H V C W
6081 TGGAGGCCTC AATGCTCTTG GACAACATGG AGGTGAGGGG
L E A S M L L D N M E V R G
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
6121 TGGAATGGTC GCCCCACTCT ATGGCGTTGA AGGAACTAAA
G M V A P L Y G V E G T K
6161 ACACCAGTTT CCCCTGGTGA AATGAGACTG AGGGATGACC
T P V S P G E M R L R D D
6201 AGAGGAAAGT CTTCAGAGAA CTAGTGAGGA ATTGTGACCT
Q R K V F R E L V R N C D L
6241 GCCCGTTTGG CTTTCGTGGC AAGTGGCCAA GGCTGGTTTG
P V W L S W Q V A K A G L
6281 AAGACGAATG ATCGTAAGTG GTGTTTTGAA GGCCCTGAGG
K T N D R K W C F E G P E
6321 AACATGAGAT CTTGAATGAC AGCGGTGAAA CAGTGAAGTG
E H E I L N D S G E T V K C
6361 CAGGGCTCCT GGAGGAGCAA AGAAGCCTCT GCGCCCAAGG
R A P G G A K K P L R P R
6401 TGGTGTGATG AAAGGGTGTC ATCTGACCAG AGTGCGCTGT
W C D E R V S S D Q S A L
6441 CTGAATTTAT TAAGTTTGCT GAAGGTAGGA GGGGAGCTGC
S E F I K F A E G R R G A A
6481 TGAAGTGCTA GTTGTGCTGA GTGAACTCCC TGATTTCCTG
E V L V V L S E L P D F L
6521 GCTAAAAAAG GTGGAGAGGC AATGGATACC ATCAGTGTGT
A K K G G E A M D T I S V
6561 TCCTCCACTC TGAGGAAGGC TCTAGGGCTT ACCGCAATGC
F L H S E E G S R A Y R N A
6601 ACTATCAATG ATGCCTGAGG CAATGACAAT AGTCATGCTG
L S M M P E A M T I V M L
6641 TTTATACTGG CTGGACTACT GACATCGGGA ATGGTCATCT
F I L A G L L T S G M V I
6681 TTTTCATGTC TCCCAAAGGC ATCAGTAGAA TGTCTATGGC
F F M S P K G I S R M S M A
6721 GATGGGCACA ATGGCCGGCT GTGGATATCT CATGTTCCTT
M G T M A G C G Y L M F L
6761 GGAGGCGTCA AACCCACTCA CATCTCCTAT GTCATGCTCA
G G V K P T H I S Y V M L
ChimeriVaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
6801 TATTCTTTGT CCTGATGGTG GTTGTGATCC CCGAGCCAGG
I F F V L M V V V I P E P G
6841 GCAACAAAGG TCCATCCAAG ACAACCAAGT GGCATACCTC
Q Q R S I Q D N Q V A Y L
6881 ATTATTGGCA TCCTGACGCT GGTTTCAGCG GTGGCAGCCA
I I G I L T L V S A V A A
6921 ACGAGCTAGG CATGCTGGAG AAAACCAAAG AGGACCTCTT
N E L G M L E K T K E D L F
6961 TGGGAAGAAG AACTTAATTC CATCTAGTGC TTCACCCTGG
G K K N L I P S S A S P W
7001 AGTTGGCCGG ATCTTGACCT GAAGCCAGGA GCTGCCTGGA
S W P D L D L K P G A A W
7041 CAGTGTACGT TGGCATTGTT ACAATGCTCT CTCCAATGTT
T V Y V G I V T M L S P M L
7081 GCACCACTGG ATCAAAGTCG AATATGGCAA CCTGTCTCTG
H H W I K V E Y G N L S L
7121 TCTGGAATAG CCCAGTCAGC CTCAGTCCTT TCTTTCATGG
S G I A Q S A S V L S F M
7161 ACAAGGGGAT ACCATTCATG AAGATGAATA TCTCGGTCAT
D K G I P F M K M N I S V I
7201 AATGCTGCTG GTCAGTGGCT GGAATTCAAT AACAGTGATG
M L L V S G W N S I T V M
7241 CCTCTGCTCT GTGGCATAGG GTGCGCCATG CTCCACTGGT
P L L C G I G C A M L H W
7281 CTCTCATTTT ACCTGGAATC AAAGCGCAGC AGTCAAAGCT
S L I L P G I K A Q Q S K L
7321 TGCACAGAGA AGGGTGTTCC ATGGCGTTGC CAAGAACCCT
A Q R R V F H G V A K N P
7361 GTGGTTGATG GGAATCCAAC AGTTGACATT GAGAAGCTC
V V D G N P T V D I E E A
7401 CTGAAATGCC TGCCCTTTAT GAGAAGAAAC TGGCTCTATA
P E M P A L Y E K K L A L Y
7441 TCTCCTTCTT GCTCTCAGCC TAGCTTCTGT TGCCATGTGC
L L L A L S L A S V A M C
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
7481 AGAACGCCCT TTTCATTGGC TGAAGGCATT GTCCTAGCAT
R T P F S L A E G I V L A
7S21 CAGCTGCCTT AGGGCCGCTC ATAGAGGGAA ACACCAGCCT
S A A L G P L I E G N T S L
7561 TCTTTGGAAT GGACCCATGG CTGTCTCCAT GACAGGAGTC
L W N G P M A V S M T G V
7601 ATGAGGGGGA ATCACTATGC TTTTGTGGGA GTCATGTACA
M R G N H Y A F V G V M Y
7641 ATCTATGGAA GATGAAAACT GGACGCCGGG GGAGCGCGAA
N L W K M K T G R R G S A N
7681 TGGAAAAACT TTGGGTGAAG TCTGGAAGAG GGAACTGAAT
G K T L G E V W K R E L N
7721 CTGTTGGACA AGCGACAGT TGAGTTGTAT AAAAGGACCG
L L D K R Q F E L Y K R T
7761 ACATTGTGGA GGTGGATCGT GATACGGCAC GCAGGCATTT
D I V E V D R D T A R R H L
7801 GGCCGAAGGG AAGGTGGACA CCGGGGTGGC GGTCTCCAGG
A E G K V D T G V A V S R
7841 GGGACCGCAA AGTTAAGGTG GTTCCATGAG CGTGGCTATG
G T A K L R W F H E R G Y
7881 TCAAGCTGGA AGGTAGGGTG ATTGACCTGG GGTGTGGCCG
V K L E G R V I D L G C G R
7921 CGGAGGCTGG TGTTACTACG CTGCTGCGCA AAAGGAAGTG
G G W C Y Y A A A Q K E V
7961 AGTGGGGTCA AAGGATTTAC TCTTGGAAGA GACGGCCATG
S G V K G F T L G R D G H
8001 AGAAACCCAT GAATGTGCAA AGTCTGGGAT GGAACATCAT
E K P M N V Q S L G W N I I
8041 CACCTTCAAG GACAAAACTG ATATCCACCG CCTAGAACCA
T F K D K T D I H R L E P
8081 GTGAAATGTG ACACCCTTTT GTGTGACATT GGAGAGTCAT
V K C D T L L C D I G E S
8121 CATCGTCATC GGTCACAGAG GGGGAAAGGA CCGTGAGAGT
S S S S V T E G E R T V R V
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
8161 TCTTGATACT GTAGAAAAAT GGCTGGCTTG TGGGGTTGAC
L D T V E K W L A C G V D
8201 AACTTCTGTG TGAAGGTGTT AGCTCCATAC ATGCCAGATG
N F C V K V L A P Y M P D
8241 TTCTTGAGAA ACTGGAATTG CTCCAAAGGA GGTTTGGCGG
V L E K L E L L Q R R F G G
8281 AACAGTGATC AGGAACCCTC TCTCCAGGAA TTCCACTCAT
T V I R N P L S R N S T H
8321 GAAATGTACT ACGTGTCTGG AGCCCGCAGC AATGTCACAT
E M Y Y V S G A R S N V T
8361 TTACTGTGAA CCAAACATCC CGCCTCCTGA TGAGGAGAAT
F T V N Q T S R L L M R R M
8401 GAGGCGTCCA ACTGGAAAAG TGACCCTGGA GGCTGACGTC
R R P T G K V T L E A D V
8441 ATCCTCCCAA TTGGGACACG CAGTGTTGAG ACAGACAAGG
I L P I G T R S V E T D K
8481 GACCCCTGGA CAAAGAGGCC ATAGAAGAAA GGGTTGAGAG
G P L D K E A I E E R V E R
8521 GATAAAATCT GAGTACATGA CCTCTTGGTT TTATGACAAT
I K S E Y M T S W F Y D N
8561 GACAACCCCT ACAGGACCTG GCACTACTGT GGCTCCTATG
D N P Y R T W H Y C G S Y
8601 TCACAAAAAC CTCCGGAAGT GCGGCGAGCA TGGTAAATGG
V T K T S G S A A S M V N G
8641 TGTTATTAAA ATTCTGACAT ATCCATGGGA CAGGATAGAG
V I K I L T Y P W D R I E
8681 GAGGTCACAA GAATGGCAAT GACTGACACA ACCCCTTTTG
E V T R M A M T D T T P F
8721 GACAGCAAAG AGTGTTTAAA GAAAAAGTTG ACACCAGAGC
G Q Q R V F K E K V D T R A
8761 AAAGGATCCA CCAGCGGGAA CTAGGAAGAT CATGAAAGTT
K D P P A G T R K I M K V
8801 GTCAACAGGT GGCTGTTCCG CCACCTGGCC AGAGAAAAGA
V N R W L F R H L A R E K
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
8841 ACCCCAGACT GTGCACAAAG GAAGAATTTA TTGCAAAAGT
N P R L C T K E E F I A K V
8881 CCGAAGTCAT GCAGCCATTG GAGCTTACCT GGAAGAACAA
R S H A A I G A Y L E E Q
8921 GAACAGTGGA AGACTGCCAA TGAGGCTGTC CAAGACCCAA
E Q W K T A N E A V Q D P
8961 AGTTCTGGGA ACTGGGGAT GAAGAAAGGA AGCTGCACCA
K F W E L V D E E R K L H Q
9001 ACAAGGCAGG TGTCGGACTT GTGTGTACAA CATGATGGGG
Q G R C R T C V Y N M M G
9041 AAAAGAGAGA AGAAGCTGTC AGAGTTTGGG AAAGCAAAGG
K R E K K L S E F G K A K
9081 GAAGCCGTGC CATATGGTAT ATGTGGCTGG GAGCGCGGTA
G S R A I W Y M W L G A R Y
9121 TCTTGAGTTT GAGGCCCTGG GATTCCTGAA TGAGGACCAT
L E F E A L G F L N E D H
9161 TGGGCTTCCA GGGAAAACTC AGGAGGAGGA GTGGAAGGCA
W A S R E N S G G G V E G
9201 TTGGCTTACA ATACCTAGGA TATGTGATCA GAGACCTGGC
I G L Q Y L G Y V I R D L A
9241 TGCAATGGAT GGTGGTGGAT TCTACGCGGA TGACACCGCT
A M D G G G F Y A D D T A
9281 GGATGGGACA CGCGCATCAC AGAGGCAGAC CTTGATGATG
G W D T R I T E A D L D D
9321 AACAGGAGAT CTTGACTAC ATGAGCCCAC ATCACAAAAA
E Q E I L N Y M S P H H K K
9361 ACTGGCACAA GCAGTGATGG AAATGACATA CAAGAACAAA
L A Q A V M E M T Y K N K
9401 GTGGTGAAAG TGTTGAGACC AGCCCCAGGA GGGAAAGCCT
V V K V L R P A P G G K A
9441 ACATGGATGT CATAAGTCGA CGAGACCAGA GAGGATCCGG
Y M D V I S R R D Q R G S G
9481 GCAGGTAGTG ACTTATGCTC TGAACACCAT CACAACTTG
Q V V T Y A L N T I T N L
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
9521 AAAGTCCAAT TGATCAGAAT GGCAGAAGCA GAGATGGTGA
K V Q L I R M A E A E M V
9561 TACATCACCA ACATGTTCAA GATTGTGATG AATCAGTTCT
I H H Q H V Q D C D E S V L
9601 GACCAGGCTG GAGGCATGGC TCACTGAGCA CGGATGTGAC
T R L E A W L T E H G C D
9641 AGACTGAAGA GGATGGCGGT GAGTGGAGAC GACTGTGTGG
R L K R M A V S G D D C V
9681 TCCGGCCCAT CGATGACAGG TTCGGCCTGG CCCTGTCCCA
V R P I D D R F G L A L S H
9721 TCTCAACGCC ATGTCCAAGG TTAGAAGGA CATATCTGAA
L N A M S K V R K D I S E
9761 TGGCAGCCAT CAAAAGGGTG GAATGATTGG GAGAATGTGC
W Q P S K G W N D W E N V
9801 CCTTCTGTTC CCACCACTTC CATGAACTAC AGCTGAAGGA
P F C S H H F H E L Q L K D
9841 TGGCAGGAGG ATTGTGGTGC CTTGCCGAGA ACAGGACGAG
G R R I V V P C R E Q D E
9881 CTCATTGGGA GAGGAAGKGGT GTCTCCAGGA AACGGCTGGA
L I G R G R V S P G N G W
9921 TGATCAAGGA AACAGCTTGC CTCAGCAAAG CCTATGCCAA
M I K E T A C L S K A Y A N
9961 CATGTGGTCA CTGATGTATT TTCACAAAAG GGACATGAGG
M W S L M Y F H K R D M R
10001 CTACTGTCAT TGGCTGTTTC CTCAGCTGTT CCCACCTCAT
L L S L A V S S A V P T S
10041 GGGTTCCACA AGGACGCACA ACATGGTCGA TTCATGGGAA
W V P Q G R T T W S I H G K
10081A AGGGGAGTGG ATGACCACGG AAGACATGCT TGAGGTGTGG
G E W M T T E D M L E V W
10121A AACAGAGTAT GGATAACCAA CAACCCACAC ATGCAGGACA
N R V W I T N N P H M Q D
10161A AGACAATGGT GAAAAAATGG AGAGATGTCC CTTATCTAAC
K T M V K K W R D V P Y L T
ChimerivaxWN02改为装瓶终产物(Run1)L/N#02H01;P/N#FP-0008
[链]
10201 CAAGAGACAA GACAAGCTGT GCGGATCACT GATTGGAATG
K R Q D K L C G S L I G M
10241 ACCAATAGGG CCACCTGGGC CTCCCACATC CATTTAGTCA
T N R A T L A S H I H L V
10281 TCCATCGTAT CCGAACGCTG ATTG3ACAGG AGAAATACAC
I H R I R T L I G Q E K Y T
10321 TGACTACCTA ACAGTCATGG ACAGGTATTC TGTGGATGCT
D Y L T V M D R Y S V D A
10361 GACCTGCAAC TGGGTGAGCT TATCTGAAAC AC1TCTAAC
D L Q L G E L I
10401 AGGAATAACC GGGATACAAA CCACGGGTGG AGAACCGGAC
10441 TCCCCACAAC CTGAAACCGG GATATAAACC ACGGCTGGAG
10481 AACCGGACTC CGCACTTAAA ATGAAACAGA AACCGGGATA
20521 AAAACTACGG ATGGAGAACC GGACTCCACA CATTGAGACA
10561 GAAGAAGTTG TCAGCCCAGA ACCCCACACG AGTTTTGCCA
10601 CTGCTAAGCT GTGAGGCAGT GCAGGCTGGG ACAGCCGACC
10641 TCCAGGTTGC GAAAAACCTG GTTTCTGGGA CCTCCCACCC
20681 CAGAGTAAAA AGAACGGAGC CTCCGCTACC ACCCTCCCAC
10721 GTGGTGGTAG AAAGACGGGG TCTAGAGGTT AGAGGAGACC
10761 CTCCAGGGAA CAAATAGTGG GACCATATTG ACGCCAGGGA
10801 AAGACCGGAG TGGTTCTCTG CTTTTCCTCC AGAGGTCTGT
10841 GAGCACAGTT TGCTCAAGAA TAAGCAGACC TTTGGATGAC
10881 AAACACAAAA CCACAA
Chimerivax WN02M66变体
[链]
SEQ ID NOS:22与23
1 NGTAAATCCT GTGTGCTAAT TGAGGTGCAT TGGTCTGCAA
41 ATCGAGTTGC TAGGCAATAA ACACATTTGG ATTAATTTTA
81 ATCGTTCGTT GAGCGATTAG CAGAGAACTG ACCAGAACAT
M
121 GTCTGGTCGT AAAGCTCAGG GAAAAACCCT GGGCGTCAAT
S G R K A Q G K T L G V N
161 ATGGTACGAC GAGGAGTTCG CTCCTTGTCA AACAAAATAA
M V R R G V R S L S N K I
201 AACAAAAAAC AAAACAAATT GGAAACAGAC CTGGACCTTC
K Q K T K Q I G N R P G P S
241 AAGAGGTGTT CAAGGATTTA TCTTTTTCTT TTTGTTCAAC
R G V Q G F I F F F L F N
281 ATTTTGACTG GAAAAAAGAT CACAGCCCAC CTAAAGAGGT
I L T G K K I T A H L K R
321 TGTGGAAAAT GCTGGACCCA AGACAAGGCT TGGCTGTTCT
L W K M L D P R Q G L A V L
361 AAGGAAAGTC AAGAGAGTGG TGGCCAGTTT GATGAGAGGA
R K V K R V V A S L M R G
401 TTGTCCTCAA GGAAACGCCG TTCCCATGAT GTTCTGACTG
L S S R K R R S H D V L T
441 TGCAATTCCT AATTTTGGGA ATGCTGTTGA TGACGGGTGG
V Q F L I L G M L L M T G G
481 AGTTACCCTC TCTAACTTCC AAGGGAAGGT GATGATGACG
V T L S N F Q G K V M M T
521 GTAAATGCTA CTGACGTCAC AGATGTCATC ACGATTCCAA
V N A T D V T D V I T I P
561 CAGCTGCTGG AAAGAACCTA TGCATTGTCA GAGCAATGGA
T A A G K N L C I V R A M D
601 TGTGGGATAC ATGTGCGATG ATACTATCAC TTATGAATGC
V G Y M C D D T I T Y E C
641 CCAGTGCTGT CGGCTGGTAA TGATCCAGAA GACATCGACT
P V L S A G N D P E D I D
Chimerivax WN02M66变体
[链]
681 GTTGGTGCAC AAAGTCAGCA GTCTACGTCA GGTATGGAAG
C W C T K S A V Y V R Y G R
721 ATGCACCAAG ACACCCACT CAAGACGCCAG TCGGAGGTCA
C T K T R H S R R S R R S
761 CTGACAGTGC AGACACACGG AGAAAGCACT CTAGCGAACA
L T V Q T H G E S T L A N
801 AGAAGGGGGC TTGGATGGAC AGCACCAAGG CCACAAGGTA
K K G A W M D S T K A T R Y
841 TTTGGTAAA ACAGAATCAT GGATCTTGAG GAACCCTGGA
L V K T E S W I L R N P G
881 TATGCCCTGG TGGCAGCCGT CATTGGTTGG ATGCTTGGGA
Y A L V A A V I G W M L G
921 GCAACACCAT GCAGAGAGTT GTGTTTGTCG TGCCATTGCT
S N T M Q R V V F V V P L L
961 TTTGGTGGCC CCAGCTTACA GCTTCAACTG CCTTGGAATG
L V A P A Y S F N C L G M
1001 AGCAACAGAG ACTTCTTGGA AGGAGTGTCT GGAGCAACAT
S N R D F L E G V S G A T
1041 GGGTGGATTT GGTTCTCGAA GGCGACAGCT GCGTGACTST
W V D L V L E G D S C V T I
1081 CATGTCTAAG GACAAGCCTA CCATCGACGT CAAGATGATG
M S K D K P T I D V K M M
1121 AATATGGAGG CGGCCAACCT GGCAGAGGTC CGCAGTTATT
N M E A A N L A E V R S Y
1161 GCTATTTGGC TACCGTCAGC GATCTCTCCA CCAAAGCTGC
C Y L A T V S D L S T K A A
1201 ATGCCCGACC ATGGGAGAAG CTCACAATGA CAAACGTGCT
C P T M G E A H N D K R A
1241 GACCCAGCTT TTGTGTGCAG ACAAGGAGTG GTGGACAGGG
D P A F V C R Q G V V D R
1281 GCTGGGGCAA CGGCTGCGGA TTTTTTGGCA AAGGATCCAT
G W G N G C G F F G K G S I
1321 TGACACATGC GCCAAATTTG CCTGCTCTAC CAAGGCAATA
D T C A K F A C S T K A I
Chimerivax WNO2M66变体
[链]
1361 GGAAGAACCA TCTTGAAAGA GAATATCAAG TACGAAGTGG
G R T I L K E N I K Y E V
1401 CCATTTTTGT CCATGGACCA ACTACTGTGG AGTCGCACGG
A I F V H G P T T V E S H G
1441 AAATTACTCC ACACAGGTTG GAGCCACTCA GGCCGGCCGA
N Y S T Q V G A T Q A G R
1481 TTCAGCATCA CTCCTGCTGC GCCTTCATAC ACACTAAAGC
F S I T P A A P S Y T L K
1521 TTGGAGAATA TGGAGAGGTG ACAGTGGACT GTGAACCACG
L G E Y G E V T V D C E P R
1561 GTCAGGGATT GACACCAATG CATACTACGT GATGACTGTT
S G I D T N A Y Y V M T V
1601 GGAACAAAGA CGTTCTTGGT CCATCGTGAG TGGTTCATGG
G T K T F L V H R E W F M
1641 ACCTCAACCT CCCTTGGAGC AGTGCTGGAA GTACTGTGTG
D L N L P W S S A G S T V W
1681 GAGGAACA GAGAGACGTTAA TGGAGTTTGA GGAACCACAC
R N R E T L M E F E E P H
1721 GCCACGAAGC AGTCTGTGAT AGCATTGGGC TCACAAGAGG
A T K Q S V I A L G S Q E
1761 GAGCTCTGCA TCAAGCTTTG GCTGGAGCCA TTCCTGTGGA
G A L H Q A L A G A I P V E
1801 ATTTTCAAGC AACACTGTCA AGTTGACGTC GGGTCATTTG
F S S N T V K L T S G H L
1841 AAGTGTAGAG TGAAGATGGA AAAATTGCAG TTGAAGGGAA
K C R V K I E K L Q L K G
1881 CAACCTATGG CGTCTGTTCA AAGGCTTTCA AGTTTCTTAG
T T Y G V C S K A F K F L R
1921 GACTCCCGTG GACACCGGTC ACGGCACTGT GGTGTTGGAA
T P V D T G H G T V V L E
1961 TTGCAGTACA CTGGCACGGA TGGACCTTGC AAAGTTCCTA
L Q Y T G T D G P C K V P
2001 TCTCGTCAGT GGCTTCATTG AACGACCTAA CGCCAGTGGG
I S S V A S L N D L T P V G
Chimerivax WNO2 M66变体
[链]
2041 CAGATTGGTC ACTGTCAACC CTTTGTTTC AGTGGCCACG
R L V T V N P F V S V A T
2081 GCCAACGCTA AGGTCCTGAT TGAATTGGAA CCACCCTTTG
A N A K V L I E L E P P F
2121 GAGACTCATA CATAGTGGTG GGCAGAGGAG AACAACAGAT
G D S Y I V V G R G E Q Q I
2161 CAATCACCAT TGGCACAAGT CTGGAAGCAG CATTGGCAAA
N H H W H K S G S S I G K
2201 GCCTTTACAA CCACCCTCAA AGGAGCGCAG AGACTAGCCG
A F T T T L K G A Q R L A
2241 CTCTAGGAGA CACAGCTTGG GACTTTGGAT CAGTTGGAGG
A L G D T A W D F G S V G G
2281 GGTGTTCACT AGTGTTGGGC GGGCTGTCCA TCAAGTGTTC
V F T S V G R A V H Q V F
2321 GGAGGAGCAT TCCGCTCACT GTTCGGAGGC ATGTCCTGGA
G G A F R S L F G G M S W
2361 TAACGCAAGG ATTGCTGGGG GCTCTCCTGT TGTGGATGGG
I T Q G L L G A L L L W M G
2401 CATCAATGCT CGTGATAGGT CCATAGCTCT CACCTTTCTC
I N A R D R S I A L T F L
2441 GCAGTTGGAG GAGTTCTGCT CITCCICTCC GTGAACGTGG
A V G G V L L F L S V N V
2481 GCGCCGATCA AGGATGCGCC ATCAACTTTG GCAAGAGAGA
G A D Q G C A I N F G K R E
2521 GCTCAAGTGC GGAGATGGTA TCTTCATATT TAGAGACTCI
L K C G D G I F I F R D S
2562 GATGACTGGC TGAACAAGIA CICATACTAT CCAGAAGAIC
D D W L N K Y S Y Y P E D
2601 CTGTGAAGCT TACATCAATA GIGAAAGCCT CTTTTGAAGA
P V K L A S I V K A S F E E
2641 AGGGAAGTGT GGCCTAAATT CAGTTGACTC CCTTGAGCAT
C K C G L N S V D S L E H
2681 GAGATGTGGA GAAGCAGGGC AGATGAGATC AATGCCATTT
E M W R S R A D E I N A I
ChimerivaxWN02M66变体
[链]
2721 TTGAGGAAAA CGAGGTGGAC ATTTCTGTTG TCGTGCAGGA
F E E N E V D I S V V V Q D
2761 TCCAAAGAAT GTTTACCAGA GAGGAACTCA TCCATTTTCC
P K N V Y Q R G T H P F S
2801 AGAATTCGGG ATGGTCTGCA GTATGGTTGG AAGACTTGGG
R I R D G L Q Y G W K T W
2841 GTAAGAACCT TGTGTTCTCC CCAGGGAGGA AGAATGGAAG
G K N L V F S P G R K N G S
2881 CTTCATCATA GATGGAAAGT CCAGGAAAGA ATGCCCGTTT
F I I D G K S R K E C P F
2921 TCAAACCGGG TCTGGAATTC TTTCCAGATA GAGGAGTTTG
S N R V W N S F Q I E E F
2961 GGACGGGAGT GTTCACCACA CGCGTGTACA TGGACGCAGT
G T G V F T T R V Y M D A V
3001 CTTTGAATAC ACCATAGACT GCGATGGATC TATCTTGGGT
F E Y T I D C D G S I L G
3041 GCAGCGGTGA ACGGAAAAAA GAGTGCCCAT GGCTCTCCAA
A A V N G K K S A H G S P
3081 CATTTTGGAT GGGAAGTCAT GAAGTAAATG GGACATGGAT
T F W M G S H E V N G T W M
3121 GATCCACACC TTGGAGGCAT TAGATTACAA GGAGTGTGAG
I H T L E A L D Y K E C E
3161 TGGCCACTGA CACATACGAT TGGAACATCA GTTGAAGAGA
W P L T H T I G T S V E E
3201 GTGAAATGTT CATGCCGAGA TCAATCGGAG GCCCAGTTAG
S E M F M P R S I G G P V S
3241 CTCTCACAAT CATATCCCTG GATACAAGGT TCAGACGAAC
S H N H I P G Y K V Q T N
3281 GGACCTTGGA TGCAGGTACC ACTAGAAGTG AAGAGAGAAG
G P W M Q V P L E V K R E
3321 CTTGCCCAGG GACTAGCGTG ATCATTGATG GCAACTGTGA
A C P G T S V I I D G N C D
3361 TGGACGGGGA AAATCAACCA GATCCACCAC GGATAGCGGG
G R G K S T R S T T D S G
Chimerivax WN02M66变体
[链]
3401 AAAGTTATTC CTGAATGGTG TTGCCGCTCC TGCACAATGC
K V I P E W C C R S C T M
3441 CGCCTGTGAG CTTCCATGGT AGTGATGGGT GTTGGTATCC
P P V S F H G S D G C W Y P
3481 CATGGAAATT AGGCCAAGGA AAACGCATGA AAGCCATCTG
M E I R P R K T H E S H L
3521 GTGCGCTCCT GGGTTACAGC TGGAGAAATA CATGCTGTCC
V R S W V T A G E I H A V
3561 CTTTTGGTTT GGTGAGCATG ATGATAGCAA TGGAAGTGGT
P P G L V S M M I A M E V V
3601 CCTAAGGAAA AGACAGGGAC CAAAGCAAAT GTTGGTTGGA
L R K R Q G P K Q M L V G
3641 GGAGTAGTGC TCTTGGGAGC AATGCTGGTC GGGCAAGTAA
G V V L L G A M L V G Q V
3681 CTCTCCTTGA TTTGCTGAAA CTCACAGTGG CTGTGGGATT
T L L D L L K L T V A V G L
3721 GCATTTCCAT GAGATGAACA ATGGAGGAGA CGCCATGTAT
H F H E M N N G G D A M Y
3761 ATGGCGTTGA TTGCTGCCTT TTCAATCAGA CCAGGGCTGC
M A L I A A F S I R P G L
3802 TCATCGGCTT TGGGCTCAGG ACCCTATGGA GCCCTCGGGA
L I G F G L R T L W S P R E
3841 ACGCCTTGTG CTGACCCTAG GAGCAGCCAT GGTGGAGATT
R L V L T L G A A M V E I
3881 GCCTTGGGTG GCGTGATGGG CGGCCTGTGG AAGTATCTAA
A L G G V M G G L W K Y L
3921 ATGCAGTTTC TCTCTGCATC CTGACAATAA ATGCTGTTGC
N A V S L C I L T I N A V A
3961 TTCTAGGAAA GCATCAAATA CCATCTTGCC CCTCATGGCT
S R K A S N T I L P L M A
4001 CTGTTGACAC CTGTCACTAT GGCTGAGGTG AGACTTGCCG
L L T P V T M A E V R L A
4041 CAATGTTCTT TTGTGCCATG GTTATCATAG GGGTCCTTCA
A M F F C A M V I I G V L H
Chimerivax WN02M66变体
[链]
4081 CCAGAATTTC AAGGACACCT CCATGCAGAA GACTATACCT
Q N F K D T S M Q K T I P
4121 CTGGTGGCCC TCACACTCAC ATCTTACCTG GGCTTGACAC
L V A L T L T S Y L G L T
4161 AACCTTTTTT GGGCCTGTGT GCATTTCTGG CAACCCGCAT
Q P F L G L C A F L A T R I
4201 ATTTGGGCGA AGGAGTATCC CAGTGAATGA GGCACTCGCA
F G R R S I P V N E A L A
4241 GCAGCTGGTC TAGTGGGAGT GCTGGCAGGA CTGGCTTTTC
A A G L V G V L A G L A F
4281 AGGAGATGGA GAACTTCCTT GGTCCGATTG CAGTTGGAGG
Q E M E N F L G P I A V G G
4321 ACTCCTGATG ATGCTGGTTA GCGTGGCTGG GAGGGTGGAT
L L M M L V S V A G R V D
4361 GGGCTAGAGC TCAAGAAGCT TGGTGAAGTT TCATGGGAAG
G L E L K K L G E V S W E
4401 AGGAGGCGGA GATCAGCGGG AGTTCCGCCC GCTATGATGT
E E A E I S G S S A R Y D V
4441 GGCACTCAGT GAACAAGGGG AGTTCAAGCT GCTTTCTGAA
A L S E Q G E F K L L S E
4481 GAGAAAGTGC CATCGGACCA GGTTGTGATG ACCTCGCTGG
E K V P V D Q V V M T S L
4521 CCTTGGTTGG GGCTGCCCTC CATCCATTTG CTCTTCTGCT
A L V G A A L H P F A L L L
4561 GGTCCTTGCT GGGTGGCTGT TTCATGTCAG GGGAGCTAGG
V L A G W L F H V R G A R
4601 AGAAGTGGGG ATGTCTTGTG GGATATTCCC ACTCCTAAGA
R S G D V L W D I P T P K
4641 TCATCGAGGA ATGTGAACAT CTGGAGGATG GGATTTATGG
I I E E C E H L E D G I Y G
4681 CATATTCCAG TCAACCTTCT TGGGGGCCTC CCAGCGAGGA
I F Q S T F L G A S Q R G
4721 GTGGGAGTGG CACAGGGAGG GGTGTTCCAC ACAATGTGGC
V G V A Q G G V F H T M W
Chimerivax WN02M66变体
[链]
4761 ATGTCACAAG AGGAGCTTTC CTTGTCAGGA ATGGCAAGAA
H V T R G A F L V R N G K K
4801 GTTGATTCCA TCTTGGGCTT CAGTAAAGGA AGACCTTGTC
L I P S W A S V K E D L V
4841 GCCTATGGTG GCTCATGGAA GTTGGAAGGC AGATGGATG
A Y G G S W K L E G R W D
4881 GAGAGGAAGA GGTCCAGTTG ATCGCGGCTG TTCCAGGAAA
G E E E V Q L I A A V P G K
4922 GAACGTGGTC AACGTCCAGA CAAAACCGAG CTTGTTCAAA
N V V N V Q T K P S L F K
4961 GTGAGGAATG GGGGAGAAAT CGGGGCTGTC GCTCTTGACT
V R N G G E I G A V A L D
5001 ATCCGAGTGG CACTTCAGGA TCTCCTATTG TTAACAGGAA
Y P S G T S G S P I V N R N
5041 CGGAGAGGTG ATTGGGCTGT ACGGCAATGG CATCCTTGTC
G E V I G L Y G N G I L V
5081 GGTGACAACT CCTTCGTGTC CGCCATATCC CAGACTGAGG
G D N S F V S A I S Q T E
5121 TGAAGGAAGA AGGAAAGGAG GAGCTCCAAG AGATCCCGAC
V K E E G K E E L Q E I P T
5161 AATGCTAAAG AAAGGAATGA CAACTGTCCT TGATTTTCAT
M L K K G M T T V L D F H
5201 CCTGGAGCTG GGAAGACAAG ACGTTTCCTC CCACAGATCT
P G A G K T R R F L P Q I
5241 TGGCCGAGTG CGCACGGAGA CGCTTGCGCA CTCTTGTGTT
L A E C A R R R L R T L V L
5281 GGCCCCCACC AGGGTTGTTC TTTCTGAAAT GAAGGAGGCT
A P T R V V L S E M K E A
5321 TTTCACGGCC TGGACGTGAA ATTCCACACA CAGGCTTTTT
F H G L D V K F H T Q A F
5361 CCGCTCACGG CAGCGGGAGA GAAGTCATTG ATGCCATGTG
S A H G S G R E V I D A M C
5401 CCATGCCACC CTAACTTACA GGATGTTGGA ACCAACTAGG
H A T L T Y R M L E P T R
Chimerivax WN02M66变体
[链]
5441 GTTGTTAACT GGGAAGTGAT CATTATGGAT GAAGCCCATT
V V N W E V I I M D E A H
5481 TTTTGGATCC AGCCAGCATA GCCGCTAGAG GTTGGGCAGC
F L D P A S I A A R G W A A
5521 GCACAGAGCT AGGGCAAATG AAAGTGCAAC AATCTTGATG
H R A R A N E S A T I L M
5561 ACAGCCACAC CGCCTGGGAC TAGTGATGAA TTTCCACATT
T A T P P G T S D E F P H
5601 CAAATGGTGA AATAGAAGAT GTTCAAACGG ACATACCCAG
S N G E I E D V Q T D I P S
5641 TGAGCCCTGG AACACAGGGC ATGACTGGAT CCTGGCTGAC
E P W N T G H D W I L A D
5681 AAAAGGCCCA CGGCATGGTT CCTTCCATCC ATCAGAGCTG
K R P T A W F L P S I R A
5721 CAAATGTCAT GGCTGCCTCT TTGCGTAAGG CTGGAAAGAG
A N V M A A S L R K A G K S
5761 TGTGGTGGTC CTGAACAGGA AAACCTTTGA GAGAGAATAC
V V V L N R K T F E R E Y
5801 CCCACGATAA AGCAGAAGAA ACCTGACTTT ATATTGGCCA
P T I K Q K K P D F I L A
5841 CTGACATAGC TGAAATGGGA GCCAACCTTT GCGTGGAGCG
T D I A E M G A N L C V E R
5881 AGTGCTGGAT TGCAGGACGG CTTTTAAGCC TGTGCTTGTG
V L D C R T A F K P V L V
5921 GATGAAGGGA GGAAGGTGGC AATAAAAGGG CCACTTCGTA
D E G R K V A I K G P L R
5961 TCTCCGCATC CTCTGCTGCT CAAAGGAGGG GGCGCATTGG
I S A S S A A Q R R G R I G
6001 GAGAAATCCC AACAGAGATG GAGACTCATA CTACTATTCT
R N P N R D G D S Y Y Y S
6041 GAGCCTACAA GTGAAAATAA TGCCCACCAC GTCTGCTGGT
E P T S E N N A H H V C W
6081 TGGAGGCCTC AATGCTCTTG GACAACATGG AGGTGAGGGG
L E A S M L L D N M E V R G
Chimerivax WN02M66变体
[链]
6121 TGGAATGGTC GCCCCACTCT ATGGCGTTGA AGGAACTAAA
G M V A P L Y G V E G T K
6161 ACACCAGTTT CCCCTGGTGA AATGAGACTG AGGGATGACC
T P V S P G E M R L R D D
6201 AGAGGAAAGT CTTCAGAGAA CTAGTGAGGA ATTGTGACCT
Q R K V F R E L V R N C D L
6241 GCCCGTTTGG CTTTCGTGGC AAGTGGCCAA GGCTGGTTTG
P V W L S W Q V A K A G L
6281 AAGACGAATG ATCGTAAGTG GTGTTTTGAA GGCCCTGAGG
K T N D R K W C F E G P E
6321 AACATGAGAT CTTGAATGAC AGCGGTGAAA CAGTGAAGTG
E H E I L N D S G E T V K C
6361 CAGGGCTCCT GGAGGAGCAA AGAAGCCTCT GCGCCCAAGG
R A P G G A K K P L R P R
6401 TGGTGTGATG AAAGGGTGTC ATCTGACCAG AGTGCGCTGT
W C D E R V S S D Q S A L
6441 CTGAATTTAT TAAGTTTGCT GAAGGTAGGA GGGGAGCTGC
S E F I K F A E G R R G A A
6481 TGAAGTGCTA GTTGTGCTGA GTGAACTCCC TGATTTCCTG
E V L V V L S E L P D F L
6521 GCTAAAAAAG GTGGAGAGGC AATGGATACC ATCAGTGTGT
A K K G G E A M D T I S V
6561 TCCTCCACTC TGAGGAAGGC TCTAGGGCTT ACCGCAATGC
F L H S E E G S R A Y R N A
6601 ACTATCAATG ATGCCTGAGG CAATGACAAT AGTCATGCTG
L S M M P E A M T I V M L
6641 TTTATACTGG CTGGACTACT GACATCGGGA ATGGTCATCT
F I L A G L L T S G M V I
6681 TTTTCATGTC TCCCAAAGGC ATCAGTAGAA TGTCTATGGC
F F M S P K G I S R M S M A
6721 GATGGGCACA ATGGCCGGCT GTGGATATCT CATGTTCCTT
M G T M A G C G Y L M F L
6761 GGAGGCGTCA AACCCACTCA CATCTCCTAT GTCATGCTCA
G G V K P T H I S Y V M L
Chimerivax WN02M66变体
[链]
6801 TATTCTTTGT CCTGATGGTG GTTGTGATCC CCGAGCCAGG
I F F V L M V V V I P E P G
6841 GCAACAAAGG TCCATCCAAG ACAACCAAGT GGCATACCTC
Q Q R S I Q D N Q V A Y L
6881 ATTATTGGCA TCCTGACGCT GGTTTCAGCG GTGGCAGCCA
I I G I L T L V S A V A A
6921 ACGAGCTAGG CATGCTGGAG AAAACCAAAG AGGACCTCTT
N E L G M L E K T K E D L F
6961 TGGGAAGAAG AACTTAATTC CATCTAGTGC TTCACCCTGG
G K K N L I P S S A S P W
7001 AGTTGGCCGG ATCTTGACCT GAAGCCAGGA GCTGCCTGGA
S W P D L D L K P G A A W
7041 CAGTGTACGT TGGCATTGTT ACAATGCTCT CTCCAATGTT
T V Y V G I V T M L S P M L
7081 GCACCACTGG ATCAAAGTCG AATATGGCAA CCTGTCTCTG
H H W I K V E Y G N L S L
7121 TCTGGAATAG CCCAGTCAGC CTCAGTCCTT TCTTTCATGG
S G I A Q S A S V L S F M
7161 ACAAGGGGAT ACCATTCATG AAGATGAATA TCTCGGTCAT
D K G I P F M K M N I S V I
7201 AATGCTGCTG GTCAGTGGCT GGAATTCAAT AACAGTGATG
M L L V S G W N S I T V M
7241 CCTCTGCTCT GTGGCATAGG GTGCGCCATG CTCCACTGGT
P L L C G I G C A M L H W
7281 CTCTCATTTT ACCTGGAATC AAAGCGCAGC AGTCAAAGCT
S L I L P G I K A Q Q S K L
7321 TGCACAGAGA AGGGTGTTCC ATGGCGTTGC CAAGAACCCT
A Q R R V F H G V A K N P
7361 GTGGTTGATG GGAATCCAAC AGTTGACATT GAGGAAGCTC
V V D G N P T V D I E E A
7401 CTGAAATGCC TGCCCTTTAT GAGAAGAAAC TGGCTCTATA
P E M P A L Y E K K L A L Y
7441 TCTCCTTCTT GCTCTCAGCC TAGCTTCTGT TGCCATGTGC
L L L A L S L A S V A M C
Chimerivax WN02M66变体
[链]
7481 AGAACGCCCT TTTCATTGGC TGAAGGCATT GTCCTAGCAT
R T P F S L A E G I V L A
7521 CAGCTGCCTT AGGGCCGCTC ATAGAGGGAA ACACCAGCCT
S A A L G P L I E G N T S L
7561 TCTTTGGAAT GGACCCATGG CTGTCTCCAT GACAGGAGTC
L W N G P M A V S M T G V
7601 ATGAGGGGGA ATCACTATGC TTTTGTGGGA GTCATGTACA
M R G N H Y A F V G V M Y
7641 ATCTATGGAA GATGAAAACT GGACGCCGGG GGAGCGCGAA
N L W K M K T G R R G S A N
7681 TGGAAAAACT TTGGGTGAAG TCTGGAAGAG GGAACTGAAT
G K T L G E V W K R E L N
7721 CTGTTGGACA AGCCACAGTT TGAGTTGTAT AAAAGGACCG
L L D K R Q F E L Y K R T
7761 ACATTGTGGA GGTGGATCGT GATACGGCAC GCAGGCATTT
D I V E V D R D T A R R H L
7801 GGCCGAACGG AACGTGGACA CCGGGGTGGC GGTCTCCAGG
A E G K V D T G V A V S R
7841 GGGACCGCAA AGTTAAGGTG GTTCCATGAG CGTGGCTATG
G T A K L R W F H E R G Y
7881 TCAAGCTGGA AGGTAGGGTG ATTGACCTGG GGTGTGGCCG
V K L E G R V I D L G C G R
7921 CGGAGGCTGG TGTTACTACG CTGCTGCGCA AAAGGAAGTG
G G W C Y Y A A A Q K E V
7961 AGTGGGGTCA AAGGATTTAC TCTTGGAAGA GACGGCCATG
S G V K G F T L G R D G H
8001 AGAAACCCAT GAATGTGCAA AGICIGGGAT GGAACATCAT
E K P M N V Q S L G W N I I
8041 CACCTTCAAG GACAAAACTG ATATCCACCG CCTAGAACCA
T F K D K T D I H R L E P
8081 GTGAAATGTG ACACCCTTTT GTGTGACATT GGAGAGTCAT
V K C D T L L C D I G E S
8121 CATCGTCATC GGTCACAGAG GGGGAAAGGA CCGTGAGAGT
S S S S V T E G E R T V R V
Chimerivax WN02M66变体
[链]
8161 TCTTGATACT GTAGAAAAAT GGCTGGCTTG TGGGGTTGAC
L D T V E K W L A C G V D
8201 AACTTCTGTG TGAAGGTGTT AGCTCCATAC ATGCCAGATG
N F C V K V L A P Y M P D
8241 TTCTTGAGAA ACTGGAATTG CTCCAAAGGA GGTTTGGCGG
V L E K L E L L Q R R F G G
8281 AACAGTGATC AGGAACCCTC TCTCCAGGAA TTCCACTCAT
T V I R N P L S R N S T H
8321 GAAATGTACT ACGTGTCTGG AGCCCGCAGC AATGTCACAT
E M Y Y V S G A R S N V T
8361 TTACTGTGAA CCAAACATCC CGCCTCCTGA TGAGGAGAAT
F T V N Q T S R L L M R R M
8401 GAGGCGTCCA ACTGGAAAAG TGACCCTGGA GGCTGACGTC
R R P T G K V T L E A D V
8441 ATCCTCCCAA TTGGGACACG CAGTGTTGAG ACAGACAAGG
I L P I G T R S V E T D K
8481 GACCCCTGGA CAAAGAGGCC ATAGAAGAAA GGGTTGAGAG
G P L D K E A I E E R V E R
8521 GATAAAATCT GAGTACATGA CCTCTTGGTT TTATGACAAT
I K S E Y M T S W F Y D N
8561 GACAACCCCT ACAGGACCTG GCACTACTGT GGCTCCTATG
D N P Y R T W H Y C G S Y
8601 TCACAAAAAC CTCCGGAAGT GCGGCGAGCA TGGTAAATGG
V T K T S G S A A S M V N G
8641 TGTTATTAAA ATTCTGACAT ATCCATGGGA CAGGATAGAG
V I K I L T Y P W D R I E
8681 GAGGTCACAA GAATGGCAAT GACTGACACA ACCCCTTTTG
E V T R M A M T D T T P F
8721 GACAGCAAAG AGTGTTTAAA GAAAAAGTTG ACACCAGAGC
G Q Q R V F K E K V D T R A
8761 AAAGGATCCA CCAGCGGGAA CTAGGAAGAT CATGAAAGTT
K D P P A G T R K I M K V
8801 GTCAACAGGT GGCTGTTCCG CCACCTGGCC AGAGAAAAGA
V N R W L F R H L A R E K
Chimerivax WN02M66变体
[链]
8841 ACCCCAGACT GTGCACAAAG GAAGAATTTA TTGCAAAAGT
N P R L C T K E E F I A K V
8881 CCGAAGTCAT GCAGCCATTG GAGCTTACCT GGAAGAACAA
R S H A A I G A Y L E E Q
8921 GAACAGTGGA AGACTGCCAA TGAGGCTGTC CAAGACCCAA
E Q W K T A N E A V Q D P
8961 AGTTCTGGGA ACTGGTGGAT GAAGAAAGGA AGCTGCACCA
K F W E L V D E E R K L H Q
9001 ACAAGGCAGG TGTCGGACTT GTGTGTACAA CATGATGGGG
Q G R C R T C V Y N M M G
9041 AAAAGAGAGA AGAAGCTGIC AGAGTTTGGG AAAGCAAAGG
K R E K K L S E F G K A K
9081 GAAGCCGTGC CATATGGTAT ATGTGGCTGG GAGCGCGGTA
G S R A I W Y M W L G A R Y
9121 TCTTGAGTTT GAGGCCCTGG GATTCCTGAA TGAGGACCAT
L E F E A L G F L N E D H
9161 TGGGCTTCCA GGGAAAACTC AGGAGGAGGA GTGGAAGGCA
W A S R E N S G G G V E G
9201 TTGGCTTACA ATACCTAGGA TATGTGATCA GAGACCTGGC
I G L Q Y L G Y V I R D L A
9241 TGCAATGGAT GGTGGTGGAT TCTACGCGGA TGACACCGCT
A M D G G G F Y A D D T A
9281 GGATGGGACA CGCGCATCAC AGAGGCAGAC CTTGATGATG
G W D T R I T E A D L D D
9321 AACAGGAGAT CTTGAACTAC ATGAGCCCAC ATCACAAAAA
E Q E I L N Y M S P H H K K
9362 ACTGGCACAA GCAGTGATGG AAATGACATA CAAGAACAAA
L A Q A V M E M T Y K N K
9401 GTGGTGAAAG TGTTGAGACC AGCCCCAGGA GGGAAAGCCT
V V K V L R P A P G G K A
9441 ACATGGATGT CATAAGTCGA CGAGACCAGA GAGGATCCGG
Y M D V I S R R D Q R G S G
9481 GCAGGTAGTG ACTTATGCTC TGAACACCAT CACCAACTTG
Q V V T Y A L N T I T N L
Chimerivax WN02M66变体
[链]
9521 AAAGTCCAAT TGATCAGAAT GGCAGAAGCA GAGATGGTGA
K V Q L I R M A E A E M V
9561 TACATCACCA ACATGTTCAA GATTGTGATG AATCAGTTCT
I H H Q H V Q D C D E S V L
9601 GACCAGGCTG GAGGCATGGC TCACTGAGCA CGGATGTGAC
T R L E A W L T E H G C D
9641 AGACTGAAGA GGATGGCCGT GAGTGGAGAC GACTGTGTGG
R L K R M A V S G D D C V
9681 TCCGGCCCAT CGATGACAGG TTCGGCCTGG CCCTGTCCCA
V R P I D D R F G L A L S H
9721 TCTCAACGCC ATGTCCAAGG TTAGAAAGGA CATATCTGAA
L N A M S K V R K D I S E
9761 TGGCAGCCAT CAAAAGGGTG GAATGATTGG GAGAATGTGC
W Q P S K G W N D W E N V
9801 CCTTCTGTTC CCACCACTTC CATGAACTAC AGCTGAAGGA
P F C S H H F H E L Q L K D
9841 TGGCAGGAGG ATTGTGGTGC CTTGCCGAGA ACAGGACGAG
G R R I V V P C R E Q D E
9881 CTCATTGGGA GAGGAAGGGT GTCTCCAGGA AACGGCTGGA
L I G R G R V S P G N G W
9921 TGATCAAGGA AACAGCTTGC CTCAGCAAAG CCTATGCCAA
M I K E T A C L S K A Y A N
9961 CATGTGGTCA CTGATGTATT TTCACAAAAG GGAGATGAGG
M W S L M Y F H K R D M R
10001 CTACTGTCAT TGGCTGTTTC CTCAGCTGTT CCCACCTCAT
L L S L A V S S A V P T S
10041 GGGTTCCACA AGGACGCACA ACATGGTCGA TTCATGGGAA
W V P Q G R T T W S I H G K
10081 AGGGCAGTCG ATGACCACGG AAGACATGCT TGAGGTGTGG
G E W M T T E D M L E V W
10121 AACAGAGTAT GGATAACCAA CAACCCACAC ATGCAGGACA
N R V W I T N N P H M Q D
10161 AGACAATGGT GAAAAAATGG AGAGATGTCC CTTATCTAAC
K T M V K K W R D V P Y L T
Chimerivax WN02M66变体
[链]
10201 CAAGAGACAA GACAAGCTGT GCGGATCACT GATTGGAATG
K R Q D K L C G S L I G M
10241 ACCAATAGGG CCACCTGGGC CTCCCACATC CATTTAGTCA
T N R A T W A S H I H L V
10281 TCCATCGTAT CCGAACGCTG ATTGGACAGG AGAAATACAC
I H R I R T L I G Q E K Y T
10321 TGACTACCTA ACAGTCATGG ACAGGTATTC TGTGGATGCT
D Y L T V M D R Y S V D A
10361 GACCTGCAAC TGGGTGAGCT TATCTGAAAC ACCATCTAAC
D L Q L G E L I
10401 AGGAATAACC GGGATACAAA CCACGGGTGG AGAACCGGAC
10441 TCCCCACAAC CTGAAACCGG GATATAAACC ACGGCTGGAG
10481 AACCGGACTC CGCACTTAAA ATAAAACAGA AACCGGGATA
10521 AAAACTACGG ATGGAGAACC GGACTCCACA CATTGAGACA
10561 GAAGAAGTTG TCAGCCCAGA ACCCCACACG AGTTTTGCCA
10601 CTGCTAAGCT GTGAGGCAGT GCAGGCTGGG ACAGCCGACC
10641 TCCAGGTTGC GAAAAACCTG GTTTCTGGGA CCTCCCACCC
10681 CAGAGTAAAA AGAACGGAGC CTCCGCTACC ACCCTCCCAC
10721 GTGGTGGTAG AAAGACGGGG TCTAGAGGTT AGAGGAGACC
10761 CTCCAGGGAA CAAATAGTGG GACCATATTG ACGCCAGGGA
10801 AAGACCGGAG TGGTTCTCTG CTTTTCCTCC AGAGGTCTGT
10841 GAGCACAGTT TGCTCAAGAA TAAGCAGACC TTTGGATGAC
10881 AAACACAAAA CCACAA
###DNA StriderTM13f7 ###
WN02x M66变体DNA比对
DNA序列 10896 bp **G TAAATCCTGT...ACAAAACCACAA linear SEQ ID NOS:36与37
DNA序列 10896 bp **G TAAATCCTGT...ACAAAACCACAA linear SEQ ID NOS:36与37
Layout: Compacted
Metbod: Blooks(Martimez)
Mismatch penalty: Smaller(1)
Gap penalty: Mediom(2)
Translation: Off SEQ ID NOS:24与38
1 **G TAAATCCTGTGTGCTAATTGAGGTGCATTGGTCTGCAAATCGAGTTGCTAGGCAATAAACACATTTGGATTAATTTTA 80
1 ................................................................................ 80
81 ATCGTTCGTTGAGCGATTAGCAGAGAACTGACCAGACATGTCTGGTCGTAAAGCTCAGGGAAAAACCCTGGGCGTCAAT 160
81 ................................................................................ 160
161 ATCGTACGACGAGGAGTTCGCTCCTTGTCAAACAAAATAAAACAAAAAACAAAACAAATTGGAAACAGACCTGGACCTTC 240
161 ................................................................................ 240
241 AAGAGGTTGTCAAGGAATTTATCTTTTTCTTTTTGTTCAACATTTTGACTGGAAAAAAGATCACAGCCCACCTAAAGAGGT 320
241 ................................................................................ 320
321 TGTGGAAAATGCTGGGACCCAAGACAAGGCTTGGCTGTTCTAAGGAAAGTCAAGAGAGTGGTGGCCAGTTTGATGAGAGGA 400
321 ................................................................................ 400
401 TTGTCCTCAAGGAAACGCCGTTCCCATGATGTTCTGACTGTGCAATTCCTAATTTTGGGAATGCTGTTGATGACGGTGG 480
401 ................................................................................ 480
481 AGTTACCCTCTCTAACTTCCAAGGGAAGGTGATGATGACGGTAAATGCTACTGAGTCACAGATGTCATCACGATTCCAA 560
481 ................................................................................ 560
561 CAGCTGCTGGAAAGAACCTATGCATTGTCAGAGCAATGGATGTGGGATACATGTGCGATGATACTATCACTTATGAATGC 640
561 ................................................................................ 640
641 CCAGTGCTGTCGGCTGGTAATGATCCAGAAGACATCGACTGTTGGTGCACAAAGTCAGCAGTCTACGTCAGGTATGGAAG 720
641 ................................................................................ 720
721 ATGCACCAAGACACGCCACTCAGACGCAGTCGGAGGTCACTGACAGTGCAGACACACGGAGAAAGCACTCTAGCGAACA 800
721 ................................................................................ 800
801 AGAAGGGGGCTTGGATGGACAGCACCAAGGCCACAAGGTATTTGGTAAAAACAGAATCATGGATCTTGAGGAACCCTGGA 880
801 ................................................................................ 880
881 TATGCCCTGGTGGCAGCCGTCATTGGTTGGATGCTTGGGAGCAACACCATGCAGAGAGTTGTHTTTGTCGTGCTATTGCT 960
881 .........................................................................C...... 960
961 TTTGGTGGCCCCAGCTTACAGCTTCAACTGCCTTGGAATGAGCAACAGAGACTTCTTGGAAGGAGTGTCGGAGCAACAT 1040
961 ................................................................................ 1040
1041 GGGTGGATTTGGTTCTCGAAGGCGACAGCTGCGTGACTATCATGTCTAAGGACAAGCCTACCATCGACGTCAAGATGATG 1120
1041 ................................................................................ 1120
1121 AATATGGAGGCGGCCAACCTGGCAGAGGTCCGCAGTTATTGCTATTTGGCTACCGTCAGCGATCTCTCCACCAAAGCTGC 1200
1121 ............................................................................... 1200
1201 ATGCCCGACCATGGGAGAAGCTCACAATGACAAACGTGCTGACCCAGTTTTGTGTGCAGACAAGGAGTGGTGGACAGGG 1280
1201 ............................................................................... 1280
1281 GCTGGGCAACGGCTGCGGATTTTTTGGCAAAGGATCCATTGACACATGCGCCAAATTTGCCTGCTCTACCAAGGCAATA 1360
1281 ............................................................................... 1360
1361 GGAAGAACCATCTTGAAAGAGAATATCAAGTACGAAGTGGCCATTTTTGTCCATGGACCACTACTGTGGAGTCGCACGG 1440
1361 ............................................................................... 1440
WN02xM66变体DNA比对
1441 AAATTACTCCACACAGGTTGGAGCCACTCAGGCCGGCCGATTCAGCATCACTCCTGCTGCGCCTTCATACACACTAAAGC 1520
1441 ................................................................................ 1520
1521 TTGGAGAATATGGAGAGGTGACAGTGGACTGTGAACCACGGTCAGGGATTGACACCAATGCATACTACGTGATGACTGTT 1600
1521 ................................................................................ 1600
1601 GGAACAAAGACGTTCTTGGTCCATCGTGAGTGGTTCATGGACCTCAACCTCCCTTGGAGCAGTGCTGGAAGTACTGTGTG 1680
1601 ................................................................................ 1680
1681 GAGGAACAGAGAGACGTTAATGGAGTTTGAGGAACCACACGCCACGAAGCAGTCTGTGATAGCATTGGGCTCACAAGAGG 1760
1681 ................................................................................ 1760
1761 GAGCTCTGCATCAAGCTTTGGCTGGAGCCATTCCTGTGGAATTTTCAAGCAACACTGTCAAGTTGACGTCGGGTCATTTG 1840
1761 ................................................................................ 1840
1841 AAGTGTAGAGTGAAGATGGAAAAATTGCAGTTGAAGGGAACAACCTATGGCGTCTGTTCAAAGGCTTTCAAGTTTCTTAG 1920
1841 ................................................................................ 1920
1921 GACTCCCGTGGACACCGGTCACGGCACTGTGGTGTTGGAATTGCAGTACACTGGCACGGATGGACCTTGCAAAGTTCCTA 2000
1921 ................................................................................ 2000
2001 TCTCGTCAGTGGCTTCATTGAACGACCTAACGCCAGTGGGCAGATTGGTCACTGTCAACCCTTTTGTTTCAGTGGCCACG 2080
2001 ................................................................................ 2080
2081 GCCAACGCTAAGGTCCTGATTGAATTGGAACCACCCTTTGGAGACTCATACATAGTGGTGGGCAGAGGAGAACAACAGAT 2160
2081 ................................................................................ 2160
2161 CAATCACCATTGGCACAAGTCTGGAAGCAGCATTGGCAAAGCCTTTACAACCACCCTCAAAGGAGCGCAGAGACTAGCCG 2240
2161 ................................................................................ 2240
2241 CTCTAGGAGACACAGCTTGGGACTTTGGATCAGTTGGAGGGGTGTTCACTAGTGTTGGGCGGGCTGTCCATCAAGTGTTC 2320
2241 ................................................................................ 2320
2321 GGAGGAGCATTCCGCTCACTGTTCGGAGGCATGTCCTGGATAACGCAAGGATTGCTGGGGGCTCTCCTGTTGTGGATGGG 2400
2321 ................................................................................ 2400
2401 CATCAATGCTCGTGATAGGTCCATAGCTCTCACGTTTCTCGCAGTTGGAGGAGTTCTGCTCTTCCTCTCCGTGAACGTGG 2480
2401 ................................................................................ 2480
2481 GCGCCGATCAAGGATGCGCCATCAACTTTGGCAAGAGAGAGCTCAAGTGCGGAGATGGTATCTTCATATTTAGAGACTCT 2560
2481 ................................................................................ 2560
2561 GATGACTGGCTGAACAAGTACTCATACTATCCAGAAGATCCTGTGAAGCTTGCATCAATAGTGAAAGCCTCTTTTGAAGA 2640
2561 ................................................................................ 2640
2641 AGGGAAGTGTGGCCTAAATTCAGTTGACTCCTTGAGCATGAGATGTGGAGAAGCAAGGGCAGATGAGATCAATGCCATTT 2720
2641 ................................................................................ 2720
2721 TTGAGGAAAACGAGGTGGACATTTCTGTTGTCGTGGAGGATCCAAAGAATGTTTACCAGAGAGGAACTCATCCATTTTCC 2800
2721 ................................................................................ 2800
2801 AGAATTCGGGATGGTCTGCAGTATGGTTGGAAGACTTGGGGTAAGAACCTTGTGTTCTCCCCAGGGAGGAAGAATGGAAG 2880
2801 ................................................................................ 2880
2881 CTTCATCATAAGATGGAAAGTCCAGGAAAGAATGCCCGTTTTCAAACCGGGTCTGGAATTCTTTCCAGATAGAGGAGTTG 2960
2881 ................................................................................ 2960
2961 GGACGGGAGTGTTCACCACACGCGTGTACATGGACCCAGTCTTTGAATACACCATAGACTGCGATGGATCTATCTTGGGT 3040
2961 ................................................................................ 3040
3041 GCAGCGGTGAACGGAAAAAAGAGTGCCCATGGCTCTCCAACATTTTGGATGGGAAGTCATGAAGTAAATGGGACATGGAT 3120
3041 ................................................................................ 3120
3121 GATCCACACCTTGGAGGCATTAGATTACAAGGAGTGTGAGTGGCCACTGACACATACGATTGGAACATCAGTTGAAGAGA 3200
3121 ................................................................................ 3200
WN02x M66变体DNA比对
3201 GTGAAATGTTCATGCCGAGATCAATCGGAGGCCCAGTTAGCTCTCACAATCATATCCCTGGATACAAGGTTCAGACGAAC 3280
3201 ................................................................................ 3280
32B1 GGACCTTGGATGCAGGTACCACTAGAAGTGAAGAGAGAAGCTTGCCCAGGGACTAGCGTGATCATTGATGGCAACTGTGA 3360
281 ................................................................................ 3360
3361 TGGACGGGGAAAATCAACCAGATCCACCACGGATAGCGGGAAAGTTATTCCTGAATGGTGTTGCCGCTCCTGCACAATGC 3440
3361 ................................................................................ 3440
3441 CGCCTGTGAGCTTCCATGGTAGTGATGGGTGTTGGTATCCCATGGAAATTAGGCCAAGGAAAACGCATGAAAGCCATCTG 3520
3441 ................................................................................ 3520
3521 GTGCGCTCCTGGGTTACACCTGGAGAAATACATGCTGTCCCTTTTGGTTTGGTGAGCATGATGATAGCAATGGAAGTGGT 3600
3521 ................................................................................ 3600
3601 CCTAAGGAAAAGACAGGGACCAAAGCAAATGTTGGTTGGAGGAGTAGTGCTCTTGGGAGCAATGCTGGTCGGGCAAGTAA 3680
3601 ................................................................................ 3680
36B1 CTCTCCCTTGATTTGCTGAAACTCACAGTGGCTGTGGGATTGCATTCCATGAGATGAACAATGGAGGAGACGCCATGTAT 3760
3681 ................................................................................ 3760
3761 ATGGCGTTGATTGCTGCCTTTTCAATCAGACCAGGGCTGCTCATCGGCTTTGGGCTCAGGACCCTATGGAGCCCTCGGGA 3840
3761 ................................................................................ 3840
3841 ACGCCTTGTGCTGACCCTAGGAGCAGCCATGGTGGAGATTGCCTTGGGTGGCGTGATGGGCGGCCTGTGGAAGTATCTAA 3920
3841 ................................................................................ 3920
3921 ATGCAGTTTCTCTCTGCATCCTGACAATAAATGCTGTTGCTTCTAGGAAAGCATCAAATACCATCTTGCCCCTCATGGCT 4000
3921 ................................................................................ 4000
4001 CTGTTGACACCTGGTCACTATGGCTGAGGTTGAGCTGCCGCAATGTTCTTTTGTGCCATGGTTATCATAGGGTTCCTTCA 4080
4001 ................................................................................. 4080
4081 CCAGAATTTCAAGGACACCTCCATGCAGAAGACTATACCTCTGGTGGCCCTCACACTCACATCTTACCTGGGCTTGACAC 4160
4081 ................................................................................ 4160
4161 AACCTTTTTTGGGCCTGTGTGCATTTCTGGCAACCGCATATTTGGGCGAAGGAGTATCCCAGTGAATGAAGGCACTCGCA 4240
4161 ................................................................................ 4240
4241 GCAGCTGGTCTAGTGGGAGTGCTGGCAGGACTGGCTTTTCAGGAGATGGAGAACTTCCTTGGTCCGATTGCAGTTGGAGG 4320
4241 ................................................................................ 4320
4321 ACTCCTGATGATGCTGGTTAGCGTGGCTGGGAGGGTGGATGGGCTAGAGCTCAAGAAGCTTGGTGAAGTTTCATGGGAAG 4400
4321 ................................................................................ 4400
4401 AGGAGGCGGAGATCAGCGGGAGTTCCGCCCGCTATGATGTGGCACTCAGTGAACAAGGGGAGTTCAAGCTGCTTTCTGAA 4480
4401 ................................................................................ 4480
4481 GAGAAAGTGCCATGGGACCAGGTTGTGATGACCTCGCTGGCCTTGGTTGGGGCTGCCCTCCATCCATTTGCTCTTCTGCT 4560
4481 ................................................................................ 4560
4561 GGTCCTTGCTGGGTGGCTGTTTCATGTCAGGGGAGCTAGGAGAAGTGGGGATGTCTTGTGGGATATTCCCACTCCTAAGA 4640
4561 ................................................................................ 4640
4641 TCATCGAGGAATGTGAACATCTGGAGGATGGGATTTATGGCATATTCCAGTCAACCTTCTTGGGGGCCTCCCAGCGAGGA 4720
4641 ................................................................................ 4720
4721 GTGGGAGTGGCACAGGGAGGGGTGTTCCACACAATGTGGCATGTCACAAGAGGAGCTTTCCTTGTCAGGAATGGCAAGAA 4800
4721 ................................................................................ 4800
4801 GTTGATTCCATCTTGGGCTTCAGTAAAGGAAGACCTTGTCGCCTATGGTGGCTCATGGAAGTTGGAAGGCAGATGGGGATG 4880
4801 ................................................................................ 4880
4881 GAGAGGAAGAGGTCCAGTTGATCGCGGCTGTTCCAGGAAAGAACGTGGTCAACGTCCAGACAAAACCGAGCTTGTTCAAA 4960
4881 ................................................................................ 4960
WN02x M66变体DNA比对
4961 GTGAGGAATGGGGGAGAAATCGGGGCTGTCGCTCTTGACTATCCGAGTGGCACTTCAGGATCTCCTATTGTTAACAGGAA 5040
4961 ................................................................................ 5040
5041 CGGAGAGGTGATTGGGCTGTACGGCAATGGCATCCTTGTCGGTGACAACTCCTTCGTGTCCGCCATATCCCAGACTGAGG 5120
5041 ................................................................................ 5120
5122 TGAAGGAAGAAGGAAAGGAGGAGCTCCAAGAGATCCCGACAATGCTAAAGAAAGGAATGACAACTGTCCTTGATTTTCGT 5200
5121 ................................................................................ 5200
5201 CCTGGAGCTGGGAAGACAAGACGTTTCCTCCCACAGATCTTGGCCGAGTGCGCACGGAGACGCTTGCGCACTCTTGTGTT 5280
5201 ............................................................................... 5280
5281 GGCCCCCACCAGGGTTGTTCTTTCTGAAATGAAGGAGGCTTTTCACGGCCTGGACGTGAAATTCCACACACAGGCTTTTT 5360
5281 ................................................................................ 5360
5361 CCGCTCACGGCAGCGGGAGAGAAGTCATTTGATGCCATGTGCCATGCCACCCTAACTTACAGATGTTGGAACCAACTAGG 5440
5361 ................................................................................ 5440
5441 GTTGTTAACTGGGAAGTGATCATTATGGATGAAGCCCATTTTTTGGATCCAGCCAGCATAGCCGCTAGAGGTTGGGCAGC 5520
5441 ................................................................................ 5520
5521 GCACAGAGCTAGGGCAAATGAAAGTGCAACAATCTTGATGACAGCCACACCGCCTGGGACTAGTGATGAATTTCCACATT 5600
5521 ................................................................................ 5600
5601 CAAATGGTGAAATAGAAGATGTTCAAACGGACATACCCAGTGAGCCCTGGAACACAGGGCATGACTGCATCCTGGCTGAC 5680
5601 ................................................................................ 5680
5681 AAAAGGCCCACGGCATGGTTCCTTCCATCCATCAGAGCTGCAAATGTCATGGCTGCCTCTTTGCGTAAGGCTGGAAAGAG 5760
5681 ................................................................................ 5760
5761 TGTGGTGGTCCTGAACAGGAAAACCTTTGAGAGAGAATACCCCACGATAAAGCAGAAGAAACCTGACTTTATATTGGCCA 5840
5761 ................................................................................ 5840
5841 CTGACATAAGCTGAAATGGGAGCCAACCTTTGCGTGGAGCGAGTGCTGGATTGCAGGACGCTTTTAAGCCTGTGCTTGTG 5920
5841 ................................................................................ 5920
5921 GATGAAGGGAGGAAGGTGGCAATAAAAGGGCCACTTCGTATCTCCGCATCCTCTGCTGCTCAAAGGAGGGGGCGCATTGG 6000
5921 ................................................................................ 6000
6001 GAGAAATCCCAACAGAGATGGAGACTCATACTACTATTCTGAGCCTACAAGTGAAAATAATGCCCAACCAGTCTGCTGGT 6080
6001 ................................................................................ 6080
6081 TGGAGGCCTCAATGCTCTTGGACAACATGGAGGTGAGGGGTGGAATGGTCGCCCCACTCTATGGCGTTGAAGGAACTAAA 6160
6081 ................................................................................ 6160
6161 ACACCAGTTTCCCCTGGTGAAATGAGACTGAGGGACGACCAGAGGAAAGTCTTCAGAGAACTAGTGAGGAATTGTGACCT 6240
6161 ................................................................................ 6240
6241 GCCCGTTTGGCTTTCGTGGCAAGTGGCCAAGGCTGGTTTGAAGACGAATGATCGTAAGTGGTGTTTTGAAGGCCCTGAGG 6320
6241 ................................................................................ 6320
6321 AACATGAGATCTTGAATGACAGCGGTGAAACAGTGAAGTGCAGGGCTCCTGGAGGAGCAAAGAAGCCTCTGCGCCCAAGG 6400
6321 ................................................................................ 6400
6401 TGGTGTGATGAAAGGGTGTCATCTGACCAGAGTGCGCTGTCTGAATTTATTAAGTTTGCTGAAGGTAGGAGGGGAGCTGC 6480
5401 ................................................................................ 6480
6481 TGAAGTGCTAGTTGTGCTGAGTGAACTCCTGATTTCCTGGCTAAAAAAGGTGGAGAGGCAAATGGATACCATCAGTGTGT 6560
6481 ................................................................................ 6560
6561 TCCTCCACTCTGAGGAAGGCTCTAGGGCTTACCGCAATGCACTATCAATGATGCCTGAGGCAATGACAATAGTCATGCTG 6640
6561 ................................................................................ 6640
6641 TTTATACTGGCTGGACTACTGACATCGGGAATGGTCATCTTTTTCATGTCTCCCAAAGGCATCAGTAGAATGTCTATGGC 6720
6641................................................................................ 6720
WN02x M66变体DNA比对
6721 GATGGGCACAATGGCCGGCTGTGGATATCTCATGTTCCTTGGAGGCGTCAAACCCACTCACATCTCCTATGTCATGCTCA 6800
6721 ................................................................................ 6800
6801 TATTCTTTGTCCTGATGGTGGTTGTGATCCCCGAGCCAGGGCAACAAAGGTCCATCCAAGACAACCAAGTGGCATACCTC 6880
6801 ................................................................................ 6880
6881 ATTATTGGCATCCTGACGCTGGTTTCAGCGGTGGCAGCCAACGAGCTAGGCATGCTGGAGAAAACCAAAGAGGACCTCTT 6960
6881 ................................................................................ 6960
6961 TGGGAAGAAGAACTTAATTCCATCTAGTGCTTCACCCTGGAGTTGGCCGGATCTTGACCTGAAGCCAGGAGCTGCCTGGA 7040
6961 ................................................................................ 7040
7041 CAGTGTACGTTGGCATTGTTACAATGCTCTCTCCAATGTTGCACCACTGGATCAAAGTCGAATATGGCAACCTGTCTCTG 7120
7041 ................................................................................ 7120
7121 TCTGGAATAGCCCAGTCAGCCTCAGTCCTTTCTTTCATGGACAAGGGGATACCATTCATGAAGATGAATATCTCGGTCAT 7200
7121 ................................................................................ 7200
7201 AATGCTGCTGGTCAGTGGCTGGAATTCAATAACAGTGATGCCTCTGCTCTGTGGCATAGGGTGCGCCATGCTCCACTGGT 7280
7201 ................................................................................ 7280
7281 CTCTCATTTTACCTGGAATCAAAGCGCAGCAGTCAAAGCTTGCACAGAGAAGGGTGTTCCATGGCGTTGCCAAGAACCCT 7360
7281 ................................................................................ 7360
7361 GTGGTTGATGGGAATCCAACAGTTGACATTGAGGAAGCTCCTGAAATGCCTGCCCTTTATGAGAAGAAACTGGCTCTATA 7440
7361 ................................................................................ 7440
7441 TCTCCTTCTTGCTCTCAGCCTAGCTTCTGTTGCCATGTGCAGAACGCCCTTTTCATTGGCTGAAGGCATTGTCCTAGCAT 7520
7441 ................................................................................ 7520
7521 CAGCTTGCCTTAGGGCCGCTCATAGAGGGAAACACCAGCCTTCTTTGGAATGGACCATGGCTGTCTCCATGACAGGAGTC 7600
7521 ................................................................................ 7600
7601 ATGAGGGGGAATCACTATGCTTTTGTGGGAGTCATGTACAATCTATGGAAGATGAAAACTGGACGCCGGGGGAGCGCGAA 7680
7601 ................................................................................ 7680
7681 TGGAAAACTTTGGGTGAAGTCTGGAAGAGGGAACTGAATCTGTTGGACAAGCTGACAGTTTGCAGTTGTATAAAGGACCG 7760
7681 ................................................................................ 7760
7761 ACATTGTGGAGGTGGATCGTGATACGGCACGCAGGCATTTGGCCGAAGGGAAGGTGGACACCGGGGTGGCGGTCTCCAGG 7840
7761 ................................................................................ 7840
7841 GGGACCGCAAAGTTAAGGTGGTTCCATGAGCGTGGCTATGTCAAGCTGGAAGGTAGGGTGATTGACCTGGGGTGTGGCCG 7920
7841 ................................................................................ 7920
7921 CGGAGGCTGGTGTTACTACGCTGCTGCGCAAAAGGAAGTGAGTGGGGTCAAAGGATTTACTCTTTGGAGAGACGGCCATG 8000
7921 ................................................................................ 8000
8001 AGAAACCCATGAATGTGCAAAGTCTGGGATGGAACATCATCACCTTCAAGGACAAAACTGATATCCACCGCCTAGAACCA 8080
8001 ................................................................................ 8080
8081 GTGAAATGTGACACCCTTTTGTGTGACATTGGAGAGTCATCATCGTCATCGGTCACAGAGGGGGAAAGGACCGTGAGAGT 8160
8081 ................................................................................ 8160
8161 TCTTGATACTGTAGAAAAATGGCTGGCTTGTGGGGTTGACAACTTCTGTGTGAAGGTGTTAGCTCCATACATGCCAGATG 8240
8161 ................................................................................ 8240
8241 TTCTTGAGAAACTGGAATTGCTCCAAAGGAGGTTTGGCGGAACAGTGATCAGGAACCCTCTCTCCAGGAATTCCACTCAT 8320
8241 ................................................................................ 8320
8321 GAAATGTACTACGTGTCTGGAGCCCGCAGCAATGTCACATTTACTGTGAACCAAACATCCCGCCTCCTGATGAGGAGAAT 8400
8321 ................................................................................ 8400
8401 GAGGCGTCCAACTGGAAAAGTGACCCTGGAGGCTGACGTCATCCTCCCAATTGGGACACGCAGTGTTGAGACAGACAAGG 8480
8401 ................................................................................ 8480
WN02x M66变体CNA比对
8481 GACCCCTGGACAAAGAGGCCATAGAAGAAAGGGTTGAGAGGATAAAATCTGAGTACATGACCTCTTGGTTTTATGACAAT 8560
8481 ................................................................................ 8560
8561 GACAACCCCTACAGGACCTGGCACTACTGTGGCTCCTATGTCACAAAAACCTCCGGAAGTGCGGCGAGCATGGTAAATGG 8640
8561 ............................................................................... 8640
8641 TGT1TATTAAAATTTCCTGACATATCCATGGGACAGGATAGAGGAGGTCACAAGAATGGCAATGACTACAACCCCTTTTG 8720
8641 ................................................................................ 8720
8721 GACAGCAAAGAGTGTTTAAAGAAAAAGTTGACACCAGAGCAAAGGATCCACCAGCGGGAACTAGGAAGATCATGAAAGTT 8800
8721 ................................................................................ 8800
8801 GTCAACAGGTGGCTGTTCCGCCACCTGGCCAGAGAAAAGAACCCCAGACTGTGCACAAAGGAAGAATTTATTGCAAAAGT 8880
8801 ................................................................................ 8880
8881 CCGAAGTCATGCAGCCATTGGAGCTTACCTGGAAGAACAAGAACAGTGGAAGACTGCCAATGAGGCTGTCCAAGACCCAA 8960
8881 ................................................................................ 8960
8961 AGTTCTGGGAACTGGTGGATGAAGAAAGGAAGCTGCACCAACAAGGCAGGTGTCGGACTTGTGTGTACAACATGATGGGG 9040
8961 ................................................................................ 9040
9041 AAAAGAGAGAAGAAGCTGTCAGAGTTTGGGAAAGCAAAGGGAAGCCGTGCCATATGGTATATCTGGCTGGGAGCGCGGTA 9120
9041 ................................................................................ 9120
9121 TCTTGAGTTTGAGGCCCTGGGATTCCTGAATGAGGACCATTGGGCTTCCAGGGAAAACTCAGGAGGAGGAGTGGAAGGCA 9200
9121 ................................................................................ 9200
9201 TTGGCTTACAATACCTAGGATATGTGATCAGAGACCTGGCTGCAATGGATGGTGGTGGATTCTACGCGGATGACACCGCT 9280
9201 ................................................................................ 9280
9281 GGATGGGACACGCGCATCACAGAGGCAGACCTTGATGATGAACAGGAGATCTTGAACTACATGAGCCCACATCACAAAAA 9360
9281 ................................................................................ 9350
9361 ACTGGCACAAGCAGTGATGGAAATGACATACAAGAACAAAGTGGTGAAAGTGTTGAGACCAGCCCCAGGAGGGAAAGCCT 9440
9361 ................................................................................ 9440
9441 ACATGGATGTCATAAGTCGACGAGACCAGAGAGGATCCGGGCAGGTAGTGACTTATGCTCTGAACACCATCACCAACTTG 9520
9441 ................................................................................ 9520
9521 AAAGTCCAATTGATCAGAATGGCAGAAGCAGAGATCGTGATACATCACCAACATGTTCAAGATTGTGATGAATCAGTTCT 9600
9521 ............................................................................... 9600
9601 GACCAGGCTGGAGGCATGGCTCACTGAGCACGGATGTGACAGACTGAAGAGGATGGCGGTGAGTGGAGACGACTGTGTGG 9680
9601 ................................................................................ 9680
9681 TCCGGCCCATCGATGACAGGTTCGGCCTGGCCCTGTCCCATCTCAACGCCATGTCCAAGGTTAGAAAGGACATATCTGAA 9760
9681 ................................................................................ 9760
9761 TGGCAGCCATCAAAAGGGTGGAATGATTGGGAGAATGTGCCCTTCTGTTCCCACCACTTCCATGAACTACAGCTGAAGGA 9840
9761 ................................................................................ 9840
9841 TGGCAGGAGGATTGTGGTGCCTTGCCGAGAACAGGACGAGCTCATTGGGAGAGGAAGGGTGTCTCCAGGAAACGGCTGGA 9920
9841 ................................................................................ 9920
9921 TGATCAAGGAAACAGCTTGCCTCAGCAAAGCCTATGCCAACATGTGGTCACTGATGTATTTTCACAAAAGGGACATGAGG 10000
9921 ................................................................................ 10000
10001 CTACTGTCATTGGCTGTTTCCTCAGCTGTTCCCACCTCATGGGTTCCACAAGGACCCACAACATGGTCGATTCATGGGAA 20080
10001 ................................................................................ 10080
10081 AGGGGAGTGGATGACCACGGAAGACATGCTTGAGGTGTGGAACAGAGTATGGATAACCAACAACCCACACATGCAGGACA 10160
10081 ................................................................................ 10160
10161 AGACAATGGTGAAAAAATGGAGAGATGTCCCTTATCTAACCAAGAGACAAGACAAGCTGTGCGGATCACTGATTGGAATG 10240
10161 ................................................................................ 10240
### DNA StriderTM13f7###周四,2004年10月21日3:10:16PM
WN02M Prot.x M66 M Prot.蛋白质比对
蛋白质 序列 75 aa SLTVQTHGBSTL...VVLLLLVAPAY S SEQ ID NOS:25与26
蛋白质 序列 75 aa SLTVQTHGKSIL...VVPLLLVAPAY S SEQ ID NOS:25与27
Layout: Standard
Hetthod: Singla Block
Blocck Length s: 6-aa
Mismatch Penalty:Smaller(1)
Gap penalty: Medium(2) SEQ ID NOS:28-30
Weighting; BLOSOH62
. 20 . 40 . 60 .
1 SLTVQTHGESTLANKKGAWMIDSTKATRYLVKTESWILLRNPGYALAAVIGWMLGSNIMQRVVFVVLLLVAPAYS 75
SLTVQTHGESTLANKKGAWMDSTKATRYLVKTESWILRNPGYALVAAVIGWMLGSNTMQRVVFVV LLLVAPAYS
1 SLTVQTEGESTLANNKKGAWMDSTKATRYLVKTESWILRNPGYALVAAVIGWMLGSNTMQVVFVVPLLLVAPAYS 75
. 20 . 40 . 60 .
%同一性=98.7(74/75)
序列表
<110>阿卡姆比斯股份有限公司(Acambis Inc.)
<120>抗日本脑炎病毒及西尼罗病毒的疫苗
<130>06132/099WO3
<140>PCT/US05/37369
<141>2005-10-19
<150>US 60/718,923
<151>2005-09-19
<150>US 60/674,546
<151>2005-04-25
<150>US 60/674,415
<151>2005-04-24
<150>US 60/620,948
<151>2004-10-21
<150>US 60/620,466
<151>2004-10-20
<160>38
<170>PatentIn version 3.3
<210>1
<211>21
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒(Yellow Fever virus)和西尼罗病毒(West Nile virus)
<400>1
cactgggaga gcttgaaggt c 21
<210>2
<211>25
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和西尼罗病毒
<400>2
aaagccagtt gcagccgcgg tttaa 25
<210>3
<211>21
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和1型登革病毒(Dengue-1 virus)
<400>3
aaggtagact ggtgggctcc c 21
<210>4
<211>26
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和1型登革病毒
<400>4
gatcctcagt accaaccgcg gtttaa 26
<210>5
<211>21
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和2型登革病毒(Dengue-2 virus)
<400>5
aaggtagatt ggtgtgcatt g 21
<210>6
<211>26
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和2型登革病毒
<400>6
aaccctcagt accacccgcg gtttaa 26
<210>7
<211>21
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和3型登革病毒(Dengue-3 virus)
<400>7
aaggtgaatt gaagtgctct a 21
<210>8
<211>25
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和3型登革病毒
<400>8
acccccagca ccacccgcgg tttaa 25
<210>9
<211>21
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和4型登革病毒(Dengue-4 virus)
<400>9
aaaaggaaca gttgttctct a 21
<210>10
<211>25
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和4型登革病毒
<400>10
acccgaagtg tcaaccgcgg tttaa 25
<210>11
<211>21
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和圣路易士脑炎病毒(St.Louis Encephalitis virus)
<400>11
aacgtgaatag ttggatagt c 21
<210>12
<211>25
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和圣路易士脑炎病毒
<400>12
accgttggtc gcacccgcgg tttaa 25
<210>13
<211>21
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和墨累谷脑炎病毒(Murray Valley Encephalitis virus)
<400>13
aatttcgaaa ggtggaaggt c 21
<210>14
<211>26
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和墨累谷脑炎病毒
<400>14
gaccggtgtt tacagccgcg gtttaa 26
<210>15
<211>21
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和蜱传脑炎病毒(Tick-Borne Encephalitis virus)
<400>15
tactgcgaac gacgttgcca c 21
<210>16
<211>25
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和蜱传脑炎病毒
<400>16
actgggaacc tcacccgcgg tttaa 25
<210>17
<211>16
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒
<400>17
ggttagagga gaccct 16
<210>18
<211>39
<212>PRT
<213>人工序列
<220>
<223>衍生于西尼罗病毒
<400>18
Ser Leu Thr Val Gln Thr His Gly Glu Ser Thr Leu Ala Asn Lys Lys
1 5 10 15
Gly Ala Trp Met Asp Ser Thr Lys Ala Thr Arg Tyr Leu Val Lys Thr
20 25 30
Glu Ser Trp Ile Leu Arg Asn
35
<210>19
<211>36
<212>PRT
<213>人工序列
<220>
<223>衍生于西尼罗病毒
<400>19
Pro Gly Tyr Ala Leu Val Ala Ala Val Ile Gly Trp Met Leu Gly Ser
1 5 10 15
Asn Thr Met Gln Arg Val Val Phe Val Val Leu Leu Leu Leu Val Ala
20 25 30
Pro Ala Tyr Ser
35
<210>20
<211>10896
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和西尼罗病毒
<220>
<221>misc_feature
<222>(1)..(1)
<223>n是a,c,g,或t
<220>
<221>CDS
<222>(119)..(10384)
<400>20
ngtaaatcct gtgtgctaat tgaggtgcat tggtctgcaa atcgagttgc taggcaataa 60
acacatttgg attaatttta atcgttcgtt gagcgattag cagagaactg accagaac 118
atg tct ggt cgt aaa gct cag gga aaa acc ctg ggc gtc aat atg gta 166
Met Ser Gly Arg Lys Ala Gln Gly Lys Thr Leu Gly Val Asn Met Val
1 5 10 15
cga cga gga gtt cgc tcc ttg tca aac aaa ata aaa caa aaa aca aaa 214
Arg Arg Gly Val Arg Ser Leu Ser Asn Lys Ile Lys Gln Lys Thr Lys
20 25 30
caa att gga aac aga cct gga cct tca aga ggt gtt caa gga ttt atc 262
Gln Ile Gly Asn Arg Pro Gly Pro Ser Arg Gly Val Gln Gly Phe Ile
35 40 45
ttt ttc ttt ttg ttc aac att ttg act gga aaa aag atc aca gcc cac 310
Phe Phe Phe Leu Phe Asn Ile Leu Thr Gly Lys Lys Ile Thr Ala His
50 55 60
cta aag agg ttg tgg aaa atg ctg gac cca aga caa ggc ttg gct gtt 358
Leu Lys Arg Leu Trp Lys Met Leu Asp Pro Arg Gln Gly Leu Ala Val
65 70 75 80
cta agg aaa gtc aag aga gtg gtg gcc agt ttg atg aga gga ttg tcc 406
Leu Arg Lys Val Lys Arg Val Val Ala Ser Leu Met Arg Gly Leu Ser
85 90 95
tca agg aaa cgc cgt tcc cat gat gtt ctg act gtg caa ttc cta att 454
Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val Gln Phe Leu Ile
100 105 110
ttg gga atg ctg ttg atg acg ggt gga gtt acc ctc tct aac ttc caa 502
Leu Gly Met Leu Leu Met Thr Gly Gly Val Thr Leu Ser Asn Phe Gln
115 120 125
ggg aag gtg atg atg acg gta aat gct act gac gtc aca gat gtc atc 550
Gly Lys Val Met Met Thr Val Asn Ala Thr Asp Val Thr Asp Val Ile
130 135 140
acg att cca aca gct gct gga aag aac cta tgc att gtc aga gca atg 598
Thr Ile Pro Thr Ala Ala Gly Lys Asn Leu Cys Ile Val Arg Ala Met
145 150 155 160
gat gtg gga tac atg tgc gat gat act atc act tat gaa tgc cca gtg 646
Asp Val Gly Tyr Met Cys Asp Asp Thr Ile Thr Tyr Glu Cys Pro Val
165 170 175
ctg tcg gct ggt aat gat cca gaa gac atc gac tgt tgg tgc aca aag 694
Leu Ser Ala Gly Asn Asp Pro Glu Asp Ile Asp Cys Trp Cys Thr Lys
180 185 190
tca gca gtc tac gtc agg tat gga aga tgc acc aag aca cgc cac tca 742
Ser Ala Val Tyr Val Arg Tyr Gly Arg Cys Thr Lys Thr Arg His Ser
195 200 205
aga cgc agt cgg agg tca ctg aca gtg cag aca cac gga gaa agc act 790
Arg Arg Ser Arg Arg Ser Leu Thr Val Gln Thr His Gly Glu Ser Thr
210 215 220
cta gcg aac aag aag ggg gct tgg atg gac agc acc aag gcc aca agg 838
Leu Ala Asn Lys Lys Gly Ala Trp Met Asp Ser Thr Lys Ala Thr Arg
225 230 235 240
tat ttg gta aaa aca gaa tca tgg atc ttg agg aac cct gga tat gcc 886
Tyr Leu Val Lys Thr Glu Ser Trp Ile Leu Arg Asn Pro Gly Tyr Ala
245 250 255
ctg gtg gca gcc gtc att ggt tgg atg ctt ggg agc aac acc atg cag 934
Leu Val Ala Ala Val Ile Gly Trp Met Leu Gly Ser Asn Thr Met Gln
260 265 270
aga gtt gtg ttt gtc gtg cta ttg ctt ttg gtg gcc cca gct tac agc 982
Arg Val Val Phe Val Val Leu Leu Leu Leu Val Ala Pro Ala Tyr Ser
275 280 285
ttc aac tgc ctt gga atg agc aac aga gac ttc ttg gaa gga gtg tct 1030
Phe Asn Cys Leu Gly Met Ser Asn Arg Asp Phe Leu Glu Gly Val Ser
290 295 300
gga gca aca tgg gtg gat ttg gtt ctc gaa ggc gac agc tgc gtg act 1078
Gly Ala Thr Trp Val Asp Leu Val Leu Glu Gly Asp Ser Cys Val Thr
305 310 315 320
atc atg tct aag gac aag cct acc atc gac gtc aag atg atg aat atg 1126
Ile Met Ser Lys Asp Lys Pro Thr Ile Asp Val Lys Met Met Asn Met
325 330 335
gag gcg gcc aac ctg gca gag gtc cgc agt tat tgc tat ttg gct acc 1174
Glu Ala Ala Asn Leu Ala Glu Val Arg Ser Tyr Cys Tyr Leu Ala Thr
340 345 350
gtc agc gat ctc tcc acc aaa gct gca tgc ccg acc atg gga gaa gct 1222
Val Ser Asp Leu Ser Thr Lys Ala Ala Cys Pro Thr Met Gly Glu Ala
355 360 365
cac aat gac aaa cgt gct gac cca gct ttt gtg tgc aga caa gga gtg 1270
His Asn Asp Lys Arg Ala Asp Pro Ala Phe Val Cys Arg Gln Gly Val
370 375 380
gtg gac agg ggc tgg ggc aac ggc tgc gga ttt ttt ggc aaa gga tcc 1318
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Phe Phe Gly Lys Gly Ser
385 390 395 400
att gac aca tgc gcc aaa ttt gcc tgc tct acc aag gca ata gga aga 1366
Ile Asp Thr Cys Ala Lys Phe Ala Cys Ser Thr Lys Ala Ile Gly Arg
405 410 415
acc atc ttg aaa gag aat atc aag tac gaa gtg gcc att ttt gtc cat 1414
Thr Ile Leu Lys Glu Asn Ile Lys Tyr Glu Val Ala Ile Phe Val His
420 425 430
gga cca act act gtg gag tcg cac gga aat tac tcc aca cag gtt gga 1462
Gly Pro Thr Thr Val Glu Ser His Gly Asn Tyr Ser Thr Gln Val Gly
435 440 445
gcc act cag gcc ggc cga ttc agc atc act cct gct gcg cct tca tac 1510
Ala Thr Gln Ala Gly Arg Phe Ser Ile Thr Pro Ala Ala Pro Ser Tyr
450 455 460
aca cta aag ctt gga gaa tat gga gag gtg aca gtg gac tgt gaa cca 1558
Thr Leu Lys Leu Gly Glu Tyr Gly Glu Val Thr Val Asp Cys Glu Pro
465 470 475 480
cgg tca ggg att gac acc aat gca tac tac gtg atg act gtt gga aca 1606
Arg Ser Gly Ile Asp Thr Asn Ala Tyr Tyr Val Met Thr Val Gly Thr
485 490 495
aag acg ttc ttg gtc cat cgt gag tgg ttc atg gac ctc aac ctc cct 1654
Lys Thr Phe Leu Val His Arg Glu Trp Phe Met Asp Leu Asn Leu Pro
500 505 510
tgg agc agt gct gga agt act gtg tgg agg aac aga gag acg tta atg 1702
Trp Ser Ser Ala Gly Ser Thr Val Trp Arg Asn Arg Glu Thr Leu Met
515 520 525
gag ttt gag gaa cca cac gcc acg aag cag tct gtg ata gca ttg ggc 1750
Glu Phe Glu Glu Pro His Ala Thr Lys Gln Ser Val Ile Ala Leu Gly
530 535 540
tca caa gag gga gct ctg cat caa gct ttg gct gga gcc att cct gtg 1798
Ser Gln Glu Gly Ala Leu His Gln Ala Leu Ala Gly Ala Ile Pro Val
545 550 555 560
gaa ttt tca agc aac act gtc aag ttg acg tcg ggt cat ttg aag tgt 1846
Glu Phe Ser Ser Asn Thr Val Lys Leu Thr Ser Gly His Leu Lys Cys
565 570 575
aga gtg aag atg gaa aaa ttg cag ttg aag gga aca acc tat ggc gtc 1894
Arg Val Lys Met Glu Lys Leu Gln Leu Lys Gly Thr Thr Tyr Gly Val
580 585 590
tgt tca aag gct ttc aag ttt ctt agg act ccc gtg gac acc ggt cac 1942
Cys Ser Lys Ala Phe Lys Phe Leu Arg Thr Pro Val Asp Thr Gly His
595 600 605
ggc act gtg gtg ttg gaa ttg cag tac act ggc acg gat gga cct tgc 1990
Gly Thr Val Val Leu Glu Leu Gln Tyr Thr Gly Thr Asp Gly Pro Cys
610 615 620
aaa gtt cct atc tcg tca gtg gct tca ttg aac gac cta acg cca gtg 2038
Lys Val Pro Ile Ser Ser Val Ala Ser Leu Asn Asp Leu Thr Pro Val
625 630 635 640
ggc aga ttg gtc act gtc aac cct ttt gtt tca gtg gcc acg gcc aac 2086
Gly Arg Leu Val Thr Val Asn Pro Phe Val Ser Val Ala Thr Ala Asn
645 650 655
gct aag gtc ctg att gaa ttg gaa cca ccc ttt gga gac tca tac ata 2134
Ala Lys Val Leu Ile Glu Leu Glu Pro Pro Phe Gly Asp Ser Tyr Ile
660 665 670
gtg gtg ggc aga gga gaa caa cag atc aat cac cat tgg cac aag tct 2182
Val Val Gly Arg Gly Glu Gln Gln Ile Asn His His Trp His Lys Ser
675 680 685
gga agc agc att ggc aaa gcc ttt aca acc acc ctc aaa gga gcg cag 2230
Gly Ser Ser Ile Gly Lys Ala Phe Thr Thr Thr Leu Lys Gly Ala Gln
690 695 700
aga cta gcc gct cta gga gac aca gct tgg gac ttt gga tca gtt gga 2278
Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly
705 710 715 720
ggg gtg ttc act agt gtt ggg cgg gct gtc cat caa gtg ttc gga gga 2326
Gly Val Phe Thr Ser Val Gly Arg Ala Val His Gln Val Phe Gly Gly
725 730 735
gca ttc cgc tca ctg ttc gga ggc atg tcc tgg ata acg caa gga ttg 2374
Ala Phe Arg Ser Leu Phe Gly Gly Met Ser Trp Ile Thr Gln Gly Leu
740 745 750
ctg ggg gct ctc ctg ttg tgg atg ggc atc aat gct cgt gat agg tcc 2422
Leu Gly Ala Leu Leu Leu Trp Met Gly Ile Asn Ala Arg Asp Arg Ser
755 760 765
ata gct ctc acg ttt ctc gca gtt gga gga gtt ctg ctc ttc ctc tcc 2470
Ile Ala Leu Thr Phe Leu Ala Val Gly Gly Val Leu Leu Phe Leu Ser
770 775 780
gtg aac gtg ggc gcc gat caa gga tgc gcc atc aac ttt ggc aag aga 2518
Val Asn Val Gly Ala Asp Gln Gly Cys Ala Ile Asn Phe Gly Lys Arg
785 790 795 800
gag ctc aag tgc gga gat ggt atc ttc ata ttt aga gac tct gat gac 2566
Glu Leu Lys Cys Gly Asp Gly Ile Phe Ile Phe Arg Asp Ser Asp Asp
805 810 815
tgg ctg aac aag tac tca tac tat cca gaa gat cct gtg aag ctt gca 2614
Trp Leu Asn Lys Tyr Ser Tyr Tyr Pro Glu Asp Pro Val Lys Leu Ala
820 825 830
tca ata gtg aaa gcc tct ttt gaa gaa ggg aag tgt ggc cta aat tca 2662
Ser Ile Val Lys Ala Ser Phe Glu Glu Gly Lys Cys Gly Leu Asn Ser
835 840 845
gtt gac tcc ctt gag cat gag atg tgg aga agc agg gca gat gag atc 2710
Val Asp Ser Leu Glu His Glu Met Trp Arg Ser Arg Ala Asp Glu Ile
850 855 860
aat gcc att ttt gag gaa aac gag gtg gac att tct gtt gtc gtg cag 2758
Asn Ala Ile Phe Glu Glu Asn Glu Val Asp Ile Ser Val Val Val Gln
865 870 875 880
gat cca aag aat gtt tac cag aga gga act cat cca ttt tcc aga att 2806
Asp Pro Lys Asn Val Tyr Gln Arg Gly Thr His Pro Phe Ser Arg Ile
885 890 895
cgg gat ggt ctg cag tat ggt tgg aag act tgg ggt aag aac ctt gtg 2854
Arg Asp Gly Leu Gln Tyr Gly Trp Lys Thr Trp Gly Lys Asn Leu Val
900 905 910
ttc tcc cca ggg agg aag aat gga agc ttc atc ata gat gga aag tcc 2902
Phe Ser Pro Gly Arg Lys Asn Gly Ser Phe Ile Ile Asp Gly Lys Ser
915 920 925
agg aaa gaa tgc ccg ttt tca aac cgg gtc tgg aat tct ttc cag ata 2950
Arg Lys Glu Cys Pro Phe Ser Asn Arg Val Trp Asn Ser Phe Gln Ile
930 935 940
gag gag ttt ggg acg gga gtg ttc acc aca cgc gtg tac atg gac gca 2998
Glu Glu Phe Gly Thr Gly Val Phe Thr Thr Arg Val Tyr Met Asp Ala
945 950 955 960
gtc ttt gaa tac acc ata gac tgc gat gga tct atc ttg ggt gca gcg 3046
Val Phe Glu Tyr Thr Ile Asp Cys Asp Gly Ser Ile Leu Gly Ala Ala
965 970 975
gtg aac gga aaa aag agt gcc cat ggc tct cca aca ttt tgg atg gga 3094
Val Asn Gly Lys Lys Ser Ala His Gly Ser Pro Thr Phe Trp Met Gly
980 985 990
agt cat gaa gta aat ggg aca tgg atg atc cac acc ttg gag gca tta 3142
Ser His Glu Val Asn Gly Thr Trp Met Ile His Thr Leu Glu Ala Leu
995 1000 1005
gat tac aag gag tgt gag tgg cca ctg aca cat acg att gga aca 3187
Asp Tyr Lys Glu Cys Glu Trp Pro Leu Thr His Thr Ile Gly Thr
1010 1015 1020
tca gtt gaa gag agt gaa atg ttc atg ccg aga tca atc gga ggc 3232
Ser Val Glu Glu Ser Glu Met Phe Met Pro Arg Ser Ile Gly Gly
1025 1030 1035
cca gtt agc tct cac aat cat atc cct gga tac aag gtt cag acg 3277
Pro Val Ser Ser His Asn His Ile Pro Gly Tyr Lys Val Gln Thr
1040 1045 1050
aac gga cct tgg atg cag gta cca cta gaa gtg aag aga gaa gct 3322
Asn Gly Pro Trp Met Gln Val Pro Leu Glu Val Lys Arg Glu Ala
1055 1060 1065
tgc cca ggg act agc gtg atc att gat ggc aac tgt gat gga cgg 3367
Cys Pro Gly Thr Ser Val Ile Ile Asp Gly Asn Cys Asp Gly Arg
1070 1075 1080
gga aaa tca acc aga tcc acc acg gat agc ggg aaa gtt att cct 3412
Gly Lys Ser Thr Arg Ser Thr Thr Asp Ser Gly Lys Val Ile Pro
1085 1090 1095
gaa tgg tgt tgc cgc tcc tgc aca atg ccg cct gtg agc ttc cat 3457
Glu Trp Cys Cys Arg Ser Cys Thr Met Pro Pro Val Ser Phe His
1100 1105 1110
ggt agt gat ggg tgt tgg tat ccc atg gaa att agg cca agg aaa 3502
Gly Ser Asp Gly Cys Trp Tyr Pro Met Glu Ile Arg Pro Arg Lys
1115 1120 1125
acg cat gaa agc cat ctg gtg cgc tcc tgg gtt aca gct gga gaa 3547
Thr His Glu Ser His Leu Val Arg Ser Trp Val Thr Ala Gly Glu
1130 1135 1140
ata cat gct gtc cct ttt ggt ttg gtg agc atg atg ata gca atg 3592
Ile His Ala Val Pro Phe Gly Leu Val Ser Met Met Ile Ala Met
1145 1150 1155
gaa gtg gtc cta agg aaa aga cag gga cca aag caa atg ttg gtt 3637
Glu Val Val Leu Arg Lys Arg Gln Gly Pro Lys Gln Met Leu Val
1160 1165 1170
gga gga gta gtg ctc ttg gga gca atg ctg gtc ggg caa gta act 3682
Gly Gly Val Val Leu Leu Gly Ala Met Leu Val Gly Gln Val Thr
1175 1180 1185
ctc ctt gat ttg ctg aaa ctc aca gtg gct gtg gga ttg cat ttc 3727
Leu Leu Asp Leu Leu Lys Leu Thr Val Ala Val Gly Leu His Phe
1190 1195 1200
cat gag atg aac aat gga gga gac gcc atg tat atg gcg ttg att 3772
His Glu Met Asn Asn Gly Gly Asp Ala Met Tyr Met Ala Leu Ile
1205 1210 1215
gct gcc ttt tca atc aga cca ggg ctg ctc atc ggc ttt ggg ctc 3817
Ala Ala Phe Ser Ile Arg Pro Gly Leu Leu Ile Gly Phe Gly Leu
1220 1225 1230
agg acc cta tgg agc cct cgg gaa cgc ctt gtg ctg acc cta gga 3862
Arg Thr Leu Trp Ser Pro Arg Glu Arg Leu Val Leu Thr Leu Gly
1235 1240 1245
gca gcc atg gtg gag att gcc ttg ggt ggc gtg atg ggc ggc ctg 3907
Ala Ala Met Val Glu Ile Ala Leu Gly Gly Val Met Gly Gly Leu
1250 1255 1260
tgg aag tat cta aat gca gtt tct ctc tgc atc ctg aca ata aat 3952
Trp Lys Tyr Leu Asn Ala Val Ser Leu Cys Ile Leu Thr Ile Asn
1265 1270 1275
gct gtt gct tct agg aaa gca tca aat acc atc ttg ccc ctc atg 3997
Ala Val Ala Ser Arg Lys Ala Ser Asn Thr Ile Leu Pro Leu Met
1280 1285 1290
gct ctg ttg aca cct gtc act atg gct gag gtg aga ctt gcc gca 4042
Ala Leu Leu Thr Pro Val Thr Met Ala Glu Val Arg Leu Ala Ala
1295 1300 1305
atg ttc ttt tgt gcc atg gtt atc ata ggg gtc ctt cac cag aat 4087
Met Phe Phe Cys Ala Met Val Ile Ile Gly Val Leu His Gln Asn
1310 1315 1320
ttc aag gac acc tcc atg cag aag act ata cct ctg gtg gcc ctc 4132
Phe Lys Asp Thr Ser Met Gln Lys Thr Ile Pro Leu Val Ala Leu
1325 1330 1335
aca ctc aca tct tac ctg ggc ttg aca caa cct ttt ttg ggc ctg 4177
Thr Leu Thr Ser Tyr Leu Gly Leu Thr Gln Pro Phe Leu Gly Leu
1340 1345 1350
tgt gca ttt ctg gca acc cgc ata ttt ggg cga agg agt atc cca 4222
Cys Ala Phe Leu Ala Thr Arg Ile Phe Gly Arg Arg Ser Ile Pro
1355 1360 1365
gtg aat gag gca ctc gca gca gct ggt cta gtg gga gtg ctg gca 4267
Val Asn Glu Ala Leu Ala Ala Ala Gly Leu Val Gly Val Leu Ala
1370 1375 1380
gga ctg gct ttt cag gag atg gag aac ttc ctt ggt ccg att gca 4312
Gly Leu Ala Phe Gln Glu Met Glu Asn Phe Leu Gly Pro Ile Ala
1385 1390 1395
gtt gga gga ctc ctg atg atg ctg gtt agc gtg gct ggg agg gtg 4357
Val Gly Gly Leu Leu Met Met Leu Val Ser Val Ala Gly Arg Val
1400 1405 1410
gat ggg cta gag ctc aag aag ctt ggt gaa gtt tca tgg gaa gag 4402
Asp Gly Leu Glu Leu Lys Lys Leu Gly Glu Val Ser Trp Glu Glu
1415 1420 1425
gag gcg gag atc agc ggg agt tcc gcc cgc tat gat gtg gca ctc 4447
Glu Ala Glu Ile Ser Gly Ser Ser Ala Arg Tyr Asp Val Ala Leu
1430 1435 1440
agt gaa caa ggg gag ttc aag ctg ctt tct gaa gag aaa gtg cca 4492
Ser Glu Gln Gly Glu Phe Lys Leu Leu Ser Glu Glu Lys Val Pro
1445 1450 1455
tgg gac cag gtt gtg atg acc tcg ctg gcc ttg gtt ggg gct gcc 4537
Trp Asp Gln Val Val Met Thr Ser Leu Ala Leu Val Gly Ala Ala
1460 1465 1470
ctc cat cca ttt gct ctt ctg ctg gtc ctt gct ggg tgg ctg ttt 4582
Leu His Pro Phe Ala Leu Leu Leu Val Leu Ala Gly Trp Leu Phe
1475 1480 1485
cat gtc agg gga gct agg aga agt ggg gat gtc ttg tgg gat att 4627
His Val Arg Gly Ala Arg Arg Ser Gly Asp Val Leu Trp Asp Ile
1490 1495 1500
ccc act cct aag atc atc gag gaa tgt gaa cat ctg gag gat ggg 4672
Pro Thr Pro Lys Ile Ile Glu Glu Cys Glu His Leu Glu Asp Gly
1505 1510 1515
att tat ggc ata ttc cag tca acc ttc ttg ggg gcc tcc cag cga 4717
Ile Tyr Gly Ile Phe Gln Ser Thr Phe Leu Gly Ala Ser Gln Arg
1520 1525 1530
gga gtg gga gtg gca cag gga ggg gtg ttc cac aca atg tgg cat 4762
Gly Val Gly Val Ala Gln Gly Gly Val Phe His Thr Met Trp His
1535 1540 1545
gtc aca aga gga gct ttc ctt gtc agg aat ggc aag aag ttg att 4807
Val Thr Arg Gly Ala Phe Leu Val Arg Asn Gly Lys Lys Leu Ile
1550 1555 1560
cca tct tgg gct tca gta aag gaa gac ctt gtc gcc tat ggt ggc 4852
Pro Ser Trp Ala Ser Val Lys Glu Asp Leu Val Ala Tyr Gly Gly
1565 1570 1575
tca tgg aag ttg gaa ggc aga tgg gat gga gag gaa gag gtc cag 4897
Ser Trp Lys Leu Glu Gly Arg Trp Asp Gly Glu Glu Glu Val Gln
1580 1585 1590
ttg atc gcg gct gtt cca gga aag aac gtg gtc aac gtc cag aca 4942
Leu Ile Ala Ala Val Pro Gly Lys Asn Val Val Asn Val Gln Thr
1595 1600 1605
aaa ccg agc ttg ttc aaa gtg agg aat ggg gga gaa atc ggg gct 4987
Lys Pro Ser Leu Phe Lys Val Arg Asn Gly Gly Glu Ile Gly Ala
1610 1615 1620
gtc gct ctt gac tat ccg agt ggc act tca gga tct cct att gtt 5032
Val Ala Leu Asp Tyr Pro Ser Gly Thr Ser Gly Ser Pro Ile Val
1625 1630 1635
aac agg aac gga gag gtg att ggg ctg tac ggc aat ggc atc ctt 5077
Asn Arg Asn Gly Glu Val Ile Gly Leu Tyr Gly Asn Gly Ile Leu
1640 1645 1650
gtc ggt gac aac tcc ttc gtg tcc gcc ata tcc cag act gag gtg 5122
Val Gly Asp Asn Ser Phe Val Ser Ala Ile Ser Gln Thr Glu Val
1655 1660 1665
aag gaa gaa gga aag gag gag ctc caa gag atc ccg aca atg cta 5167
Lys Glu Glu Gly Lys Glu Glu Leu Gln Glu Ile Pro Thr Met Leu
1670 1675 1680
aag aaa gga atg aca act gtc ctt gat ttt cat cct gga gct ggg 5212
Lys Lys Gly Met Thr Thr Val Leu Asp Phe His Pro Gly Ala Gly
1685 1690 1695
aag aca aga cgt ttc ctc cca cag atc ttg gcc gag tgc gca cgg 5257
Lys Thr Arg Arg Phe Leu Pro Gln Ile Leu Ala Glu Cys Ala Arg
1700 1705 1710
aga cgc ttg cgc act ctt gtg ttg gcc ccc acc agg gtt gtt ctt 5302
Arg Arg Leu Arg Thr Leu Val Leu Ala Pro Thr Arg Val Val Leu
1715 1720 1725
tct gaa atg aag gag gct ttt cac ggc ctg gac gtg aaa ttc cac 5347
Ser Glu Met Lys Glu Ala Phe His Gly Leu Asp Val Lys Phe His
1730 1735 1740
aca cag gct ttt tcc gct cac ggc agc ggg aga gaa gtc att gat 5392
Thr Gln Ala Phe Ser Ala His Gly Ser Gly Arg Glu Val Ile Asp
1745 1750 1755
gcc atg tgc cat gcc acc cta act tac agg atg ttg gaa cca act 5437
Ala Met Cys His Ala Thr Leu Thr Tyr Arg Met Leu Glu Pro Thr
1760 1765 1770
agg gtt gtt aac tgg gaa gtg atc att atg gat gaa gcc cat ttt 5482
Arg Val Val Asn Trp Glu Val Ile Ile Met Asp Glu Ala His Phe
1775 1780 1785
ttg gat cca gec agc ata gcc gct aga ggt tgg gca gcg cac aga 5527
Leu Asp Pro Ala Ser Ile Ala Ala Arg Gly Trp Ala Ala His Arg
1790 1795 1800
gct agg gca aat gaa agt gca aca atc ttg atg aca gcc aca ccg 5572
Ala Arg Ala Asn Glu Ser Ala Thr Ile Leu Met Thr Ala Thr Pro
1805 1810 1815
cct ggg act agt gat gaa ttt cca cat tca aat ggt gaa ata gaa 5617
Pro Gly Thr Ser Asp Glu Phe Pro His Ser Asn Gly Glu Ile Glu
1820 1825 1830
gat gtt caa acg gac ata ccc agt gag ccc tgg aae aca ggg cat 5662
Asp Val Gln Thr Asp Ile Pro Ser Glu Pro Trp Asn Thr Gly His
1835 1840 1845
gac tgg atc ctg gct gac aaa agg ccc acg gca tgg ttc ctt cca 5707
Asp Trp Ile Leu Ala Asp Lys Arg Pro Thr Ala Trp Phe Leu Pro
1850 1855 1860
tcc atc aga gct gca aat gtc atg gct gcc tct ttg cgt aag gct 5752
Ser Ile Arg Ala Ala Asn Val Met Ala Ala Ser Leu Arg Lys Ala
1865 1870 1875
gga aag agt gtg gtg gtc ctg aac agg aaa acc ttt gag aga gaa 5797
Gly Lys Ser Val Val Val Leu Asn Arg Lys Thr Phe Glu Arg Glu
1880 1885 1890
tac ccc acg ata aag cag aag aaa cct gac ttt ata ttg gcc act 5842
Tyr Pro Thr Ile Lys Gln Lys Lys Pro Asp Phe Ile Leu Ala Thr
1895 1900 1905
gac ata gct gaa atg gga gcc aac ctt tgc gtg gag cga gtg ctg 5887
Asp Ile Ala Glu Met Gly Ala Asn Leu Cys Val Glu Arg Val Leu
1910 1915 1920
gat tgc agg acg gct ttt aag cct gtg ctt gtg gat gaa ggg agg 5932
Asp Cys Arg Thr Ala Phe Lys Pro Val Leu Val Asp Glu Gly Arg
1925 1930 1935
aag gtg gca ata aaa ggg cca ctt cgt atc tcc gca tcc tct gct 5977
Lys Val Ala Ile Lys Gly Pro Leu Arg Ile Ser Ala Ser Ser Ala
1940 1945 1950
gct caa agg agg ggg cgc att ggg aga aat ccc aac aga gat gga 6022
Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro Asn Arg Asp Gly
1955 1960 1965
gac tca tac tac tat tct gag cct aca agt gaa aat aat gcc cac 6067
Asp Ser Tyr Tyr Tyr Ser Glu Pro Thr Ser Glu Asn Asn Ala His
1970 1975 1980
cac gtc tgc tgg ttg gag gcc tca atg ctc ttg gac aac atg gag 6112
His Val Cys Trp Leu Glu Ala Ser Met Leu Leu Asp Asn Met Glu
1985 1990 1995
gtg agg ggt gga atg gtc gcc cca ctc tat ggc gtt gaa gga act 6157
Val Arg Gly Gly Met Val Ala Pro Leu Tyr Gly Val Glu Gly Thr
2000 2005 2010
aaa aca cca gtt tcc cct ggt gaa atg aga ctg agg gat gac cag 6202
Lys Thr Pro Val Ser Pro Gly Glu Met Arg Leu Arg Asp Asp Gln
2015 2020 2025
agg aaa gtc ttc aga gaa cta gtg agg aat tgt gac ctg ccc gtt 6247
Arg Lys Val Phe Arg Glu Leu Val Arg Asn Cys Asp Leu Pro Val
2030 2035 2040
tgg ctt tcg tgg caa gtg gcc aag gct ggt ttg aag acg aat gat 6292
Trp Leu Ser Trp Gln Val Ala Lys Ala Gly Leu Lys Thr Asn Asp
2045 2050 2055
cgt aag tgg tgt ttt gaa ggc cct gag gaa cat gag atc ttg aat 6337
Arg Lys Trp Cys Phe Glu Gly Pro Glu Glu His Glu Ile Leu Asn
2060 2065 2070
gac agc ggt gaa aca gtg aag tgc agg gct cct gga gga gca aag 6382
Asp Ser Gly Glu Thr Val Lys Cys Arg Ala Pro Gly Gly Ala Lys
2075 2080 2085
aag cct ctg cgc cca agg tgg tgt gat gaa agg gtg tca tct gac 6427
Lys Pro Leu Arg Pro Arg Trp Cys Asp Glu Arg Val Ser Ser Asp
2090 2095 2100
cag agt gcg ctg tct gaa ttt att aag ttt gct gaa ggt agg agg 6472
Gln Ser Ala Leu Ser Glu Phe Ile Lys Phe Ala Glu Gly Arg Arg
2105 2110 2115
gga gct gct gaa gtg cta gtt gtg ctg agt gaa ctc cct gat ttc 6517
Gly Ala Ala Glu Val Leu Val Val Leu Ser Glu Leu Pro Asp Phe
2120 2125 2130
ctg gct aaa aaa ggt gga gag gca atg gat acc atc agt gtg ttc 6562
Leu Ala Lys Lys Gly Gly Glu Ala Met Asp Thr Ile Ser Val Phe
2135 2140 2145
ctc cac tct gag gaa ggc tct agg gct tac cgc aat gca cta tca 6607
Leu His Ser Glu Glu Gly Ser Arg Ala Tyr Arg Asn Ala Leu Ser
2150 2155 2160
atg atg cct gag gca atg aca ata gtc atg ctg ttt ata ctg gct 6652
Met Met Pro Glu Ala Met Thr Ile Val Met Leu Phe Ile Leu Ala
2165 2170 2175
gga cta ctg aca tcg gga atg gtc atc ttt ttc atg tct ccc aaa 6697
Gly Leu Leu Thr Ser Gly Met Val Ile Phe Phe Met Ser Pro Lys
2180 2185 2190
ggc atc agt aga atg tct atg gcg atg ggc aca atg gcc ggc tgt 6742
Gly Ile Ser Arg Met Ser Met Ala Met Gly Thr Met Ala Gly Cys
2195 2200 2205
gga tat ctc atg ttc ctt gga ggc gtc aaa ccc act cac atc tcc 6787
Gly Tyr Leu Met Phe Leu Gly Gly Val Lys Pro Thr His Ile Ser
2210 2215 2220
tat gtc atg ctc ata ttc ttt gtc ctg atg gtg gtt gtg atc ccc 6832
Tyr Val Met Leu Ile Phe Phe Val Leu Met Val Val Val Ile Pro
2225 2230 2235
gag cca ggg caa caa agg tcc atc caa gac aac caa gtg gca tac 6877
Glu Pro Gly Gln Gln Arg Ser Ile Gln Asp Asn Gln Val Ala Tyr
2240 2245 2250
ctc att att ggc atc ctg acg ctg gtt tca gcg gtg gca gcc aac 6922
Leu Ile Ile Gly Ile Leu Thr Leu Val Ser Ala Val Ala Ala Asn
2255 2260 2265
gag cta ggc atg ctg gag aaa acc aaa gag gac ctc ttt ggg aag 6967
Glu Leu Gly Met Leu Glu Lys Thr Lys Glu Asp Leu Phe Gly Lys
2270 2275 2280
aag aac tta att cca tct agt gct tca ccc tgg agt tgg ccg gat 7012
Lys Asn Leu Ile Pro Ser Ser Ala Ser Pro Trp Ser Trp Pro Asp
2285 2290 2295
ctt gac ctg aag cca gga gct gcc tgg aca gtg tac gtt ggc att 7057
Leu Asp Leu Lys Pro Gly Ala Ala Trp Thr Val Tyr Val Gly Ile
2300 2305 2310
gtt aca atg ctc tct cca atg ttg cac cac tgg atc aaa gtc gaa 7102
Val Thr Met Leu Ser Pro Met Leu His His Trp Ile Lys Val Glu
2315 2320 2325
tat ggc aac ctg tct ctg tct gga ata gcc cag tca gcc tca gtc 7147
Tyr Gly Asn Leu Ser Leu Ser Gly Ile Ala Gln Ser Ala Ser Val
2330 2335 2340
ctt tct ttc atg gac aag ggg ata cca ttc atg aag atg aat atc 7192
Leu Ser Phe Met Asp Lys Gly Ile Pro Phe Met Lys Met Asn Ile
2345 2350 2355
tcg gtc ata atg ctg ctg gtc agt ggc tgg aat tca ata aca gtg 7237
Ser Val Ile Met Leu Leu Val Ser Gly Trp Asn Ser Ile Thr Val
2360 2365 2370
atg cct ctg ctc tgt ggc ata ggg tgc gcc atg ctc cac tgg tct 7282
Met Pro Leu Leu Cys Gly Ile Gly Cys Ala Met Leu His Trp Ser
2375 2380 2385
ctc att tta cct gga atc aaa gcg cag cag tca aag ctt gca cag 7327
Leu Ile Leu Pro Gly Ile Lys Ala Gln Gln Ser Lys Leu Ala Gln
2390 2395 2400
aga agg gtg ttc cat ggc gtt gcc aag aac cct gtg gtt gat ggg 7372
Arg Arg Val Phe His Gly Val Ala Lys Asn Pro Val Val Asp Gly
2405 2410 2415
aat cca aca gtt gac att gag gaa gct cct gaa atg cct gcc ctt 7417
Asn Pro Thr Val Asp Ile Glu Glu Ala Pro Glu Met Pro Ala Leu
2420 2425 2430
tat gag aag aaa ctg gct cta tat ctc ctt ctt gct ctc agc cta 7462
Tyr Glu Lys Lys Leu Ala Leu Tyr Leu Leu Leu Ala Leu Ser Leu
2435 2440 2445
gct tct gtt gcc atg tgc aga acg ccc ttt tca ttg gct gaa ggc 7507
Ala Ser Val Ala Met Cys Arg Thr Pro Phe Ser Leu Ala Glu Gly
2450 2455 2460
att gtc cta gca tca gct gcc tta ggg ccg ctc ata gag gga aac 7552
Ile Val Leu Ala Ser Ala Ala Leu Gly Pro Leu Ile Glu Gly Asn
2465 2470 2475
acc agc ctt ctt tgg aat gga ccc atg gct gtc tcc atg aca gga 7597
Thr Ser Leu Leu Trp Asn Gly Pro Met Ala Val Ser Met Thr Gly
2480 2485 2490
gtc atg agg ggg aat cac tat gct ttt gtg gga gtc atg tac aat 7642
Val Met Arg Gly Asn His Tyr Ala Phe Val Gly Val Met Tyr Asn
2495 2500 2505
cta tgg aag atg aaa act gga cgc cgg ggg agc gcg aat gga aaa 7687
Leu Trp Lys Met Lys Thr Gly Arg Arg Gly Ser Ala Asn Gly Lys
2510 2515 2520
act ttg ggt gaa gtc tgg aag agg gaa ctg aat ctg ttg gac aag 7732
Thr Leu Gly Glu Val Trp Lys Arg Glu Leu Asn Leu Leu Asp Lys
2525 2530 2535
cga cag ttt gag ttg tat aaa agg acc gac att gtg gag gtg gat 7777
Arg Gln Phe Glu Leu Tyr Lys Arg Thr Asp Ile Val Glu Val Asp
2540 2545 2550
cgt gat acg gca cgc agg cat ttg gcc gaa ggg aag gtg gac acc 7822
Arg Asp Thr Ala Arg Arg His Leu Ala Glu Gly Lys Val Asp Thr
2555 2560 2565
ggg gtg gcg gtc tcc agg ggg acc gca aag tta agg tgg ttc cat 7867
Gly Val Ala Val Ser Arg Gly Thr Ala Lys Leu Arg Trp Phe His
2570 2575 2580
gag cgt ggc tat gtc aag ctg gaa ggt agg gtg att gac ctg ggg 7912
Glu Arg Gly Tyr Val Lys Leu Glu Gly Arg Val Ile Asp Leu Gly
2585 2590 2595
tgt ggc cgc gga ggc tgg tgt tac tac gct gct gcg caa aag gaa 7957
Cys Gly Arg Gly Gly Trp Cys Tyr Tyr Ala Ala Ala Gln Lys Glu
2600 2605 2610
gtg agt ggg gtc aaa gga ttt act ctt gga aga gac ggc cat gag 8002
Val Ser Gly Val Lys Gly Phe Thr Leu Gly Arg Asp Gly His Glu
2615 2620 2625
aaa ccc atg aat gtg caa agt ctg gga tgg aac atc atc acc ttc 8047
Lys Pro Met Asn Val Gln Ser Leu Gly Trp Asn Ile Ile Thr Phe
2630 2635 2640
aag gac aaa act gat atc cac cgc cta gaa cca gtg aaa tgt gac 8092
Lys Asp Lys Thr Asp Ile His Arg Leu Glu Pro Val Lys Cys Asp
2645 2650 2655
acc ctt ttg tgt gac att gga gag tca tca tcg tca tcg gtc aca 8137
Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Ser Ser Val Thr
2660 2665 2670
gag ggg gaa agg acc gtg aga gtt ctt gat act gta gaa aaa tgg 8182
Glu Gly Glu Arg Thr Val Arg Val Leu Asp Thr Val Glu Lys Trp
2675 2680 2685
ctg gct tgt ggg gtt gac aac ttc tgt gtg aag gtg tta gct cca 8227
Leu Ala Cys Gly Val Asp Asn Phe Cys Val Lys Val Leu Ala Pro
2690 2695 2700
tac atg cca gat gtt ctt gag aaa ctg gaa ttg ctc caa agg agg 8272
Tyr Met Pro Asp Val Leu Glu Lys Leu Glu Leu Leu Gln Arg Arg
2705 2710 2715
ttt ggc gga aca gtg atc agg aac cct ctc tcc agg aat tcc act 8317
Phe Gly Gly Thr Val Ile Arg Asn Pro Leu Ser Arg Asn Ser Thr
2720 2725 2730
cat gaa atg tac tac gtg tct gga gcc cgc agc aat gtc aca ttt 8362
His Glu Met Tyr Tyr Val Ser Gly Ala Arg Ser Asn Val Thr Phe
2735 2740 2745
act gtg aac caa aca tcc cgc ctc ctg atg agg aga atg agg egt 8407
Thr Val Asn Gln Thr Ser Arg Leu Leu Met Arg Arg Met Arg Arg
2750 2755 2760
cca act gga aaa gtg acc ctg gag gct gac gtc atc ctc cca att 8452
Pro Thr Gly Lys Val Thr Leu Glu Ala Asp Val Ile Leu Pro Ile
2765 2770 2775
ggg aca cgc agt gtt gag aca gac aag gga ccc ctg gac aaa gag 8497
Gly Thr Arg Ser Val Glu Thr Asp Lys Gly Pro Leu Asp Lys Glu
2780 2785 2790
gcc ata gaa gaa agg gtt gag agg ata aaa tct gag tac atg acc 8542
Ala Ile Glu Glu Arg Val Glu Arg Ile Lys Ser Glu Tyr Met Thr
2795 2800 2805
tct tgg ttt tat gac aat gac aac ccc tac agg acc tgg cac tac 8587
Ser Trp Phe Tyr Asp Asn Asp Asn Pro Tyr Arg Thr Trp His Tyr
2810 2815 2820
tgt ggc tcc tat gtc aca aaa acc tcc gga agt gcg gcg agc atg 8632
Cys Gly Ser Tyr Val Thr Lys Thr Ser Gly Ser Ala Ala Ser Met
2825 2830 2835
gta aat ggt gtt att aaa att ctg aca tat cca tgg gac agg ata 8677
Val Asn Gly Val Ile Lys Ile Leu Thr Tyr Pro Trp Asp Arg Ile
2840 2845 2850
gag gag gtc aca aga atg gca atg act gac aca acc cct ttt gga 8722
Glu Glu Val Thr Arg Met Ala Met Thr Asp Thr Thr Pro Phe Gly
2855 2860 2865
cag caa aga gtg ttt aaa gaa aaa gtt gac acc aga gca aag gat 8767
Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Ala Lys Asp
2870 2875 2880
cca cca gcg gga act agg aag atc atg aaa gtt gtc aac agg tgg 8812
Pro Pro Ala Gly Thr Arg Lys Ile Met Lys Val Val Asn Arg Trp
2885 2890 2895
ctg ttc cgc cac ctg gcc aga gaa aag aac ccc aga ctg tgc aca 8857
Leu Phe Arg His Leu Ala Arg Glu Lys Asn Pro Arg Leu Cys Thr
2900 2905 2910
aag gaa gaa ttt att gca aaa gtc cga agt cat gca gcc att gga 8902
Lys Glu Glu Phe Ile Ala Lys Val Arg Ser His Ala Ala Ile Gly
2915 2920 2925
gct tac ctg gaa gaa caa gaa cag tgg aag act gcc aat gag gct 8947
Ala Tyr Leu Glu Glu Gln Glu Gln Trp Lys Thr Ala Asn Glu Ala
2930 2935 2940
gtc caa gac cca aag ttc tgg gaa ctg gtg gat gaa gaa agg aag 8992
Val Gln Asp Pro Lys Phe Trp Glu Leu Val Asp Glu Glu Arg Lys
2945 2950 2955
ctg cac caa caa ggc agg tgt cgg act tgt gtg tac aac atg atg 9037
Leu His Gln Gln Gly Arg Cys Arg Thr Cys Val Tyr Asn Met Met
2960 2965 2970
ggg aaa aga gag aag aag ctg tca gag ttt ggg aaa gca aag gga 9082
Gly Lys Arg Glu Lys Lys Leu Ser Glu Phe Gly Lys Ala Lys Gly
2975 2980 2985
agc cgt gcc ata tgg tat atg tgg ctg gga gcg cgg tat ctt gag 9127
Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Tyr Leu Glu
2990 2995 3000
ttt gag gcc ctg gga ttc ctg aat gag gac cat tgg gct tcc agg 9172
Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Ala Ser Arg
3005 3010 3015
gaa aac tca gga gga gga gtg gaa ggc att ggc tta caa tac cta 9217
Glu Asn Ser Gly Gly Gly Val Glu Gly Ile Gly Leu Gln Tyr Leu
3020 3025 3030
gga tat gtg atc aga gac ctg gct gca atg gat ggt ggt gga ttc 9262
Gly Tyr Val Ile Arg Asp Leu Ala Ala Met Asp Gly Gly Gly Phe
3035 3040 3045
tac gcg gat gac acc gct gga tgg gac acg cgc atc aca gag gca 9307
Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu Ala
3050 3055 3060
gac ctt gat gat gaa cag gag atc ttg aac tac atg agc cca cat 9352
Asp Leu Asp Asp Glu Gln Glu Ile Leu Asn Tyr Met Ser Pro His
3065 3070 3075
cac aaa aaa ctg gca caa gca gtg atg gaa atg aca tac aag aac 9397
His Lys Lys Leu Ala Gln Ala Val Met Glu Met Thr Tyr Lys Asn
3080 3085 3090
aaa gtg gtg aaa gtg ttg aga cca gcc cca gga ggg aaa gcc tac 9442
Lys Val Val Lys Val Leu Arg Pro Ala Pro Gly Gly Lys Ala Tyr
3095 3100 3105
atg gat gtc ata agt cga cga gac cag aga gga tcc ggg cag gta 9487
Mct Asp Val Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly Gln Val
3110 3115 3120
gtg act tat gct ctg aac acc atc acc aac ttg aaa gtc caa ttg 9532
Val Thr Tyr Ala Leu Asn Thr Ile Thr Asn Leu Lys Val Gln Leu
3125 3130 3135
atc aga atg gca gaa gca gag atg gtg ata cat cac caa cat gtt 9577
Ile Arg Met Ala Glu Ala Glu Met Val Ile His His Gln His Val
3140 3145 3150
caa gat tgt gat gaa tca gtt ctg acc agg ctg gag gca tgg ctc 9622
Gln Asp Cys Asp Glu Ser Val Leu Thr Arg Leu Glu Ala Trp Leu
3155 3160 3165
act gag cac gga tgt gac aga ctg aag agg atg gcg gtg agt gga 9667
Thr Glu His Gly Cys Asp Arg Leu Lys Arg Met Ala Val Ser Gly
3170 3175 3180
gac gac tgt gtg gtc cgg ccc atc gat gac agg ttc ggc ctg gcc 9712
Asp Asp Cys Val Val Arg Pro Ile Asp Asp Arg Phe Gly Leu Ala
3185 3190 3195
ctg tcc cat ctc aac gcc atg tcc aag gtt aga aag gac ata tct 9757
Lcu Ser His Leu Asn Ala Met Ser Lys Val Arg Lys Asp Ile Ser
3200 3205 3210
gaa tgg cag cca tca aaa ggg tgg aat gat tgg gag aat gtg ccc 9802
Glu Trp Gln Pro Ser Lys Gly Trp Asn Asp Trp Glu Asn Val Pro
3215 3220 3225
ttc tgt tcc cac cac ttc cat gaa cta cag ctg aag gat ggc agg 9847
Phe Cys Ser His His Phe His Glu Leu Gln Leu Lys Asp Gly Arg
3230 3235 3240
agg att gtg gtg cct tgc cga gaa cag gac gag ctc att ggg aga 9892
Arg Ile Val Val Pro Cys Arg Glu Gln Asp Glu Leu Ile Gly Arg
3245 3250 3255
gga agg gtg tct cca gga aac ggc tgg atg atc aag gaa aca gct 9937
Gly Arg Val Ser Pro Gly Asn Gly Trp Met Ile Lys Glu Thr Ala
3260 3265 3270
tgc ctc agc aaa gcc tat gcc aac atg tgg tca ctg atg tat ttt 9982
Cys Leu Ser Lys Ala Tyr Ala Asn Met Trp Ser Leu Met Tyr Phe
3275 3280 3285
cac aaa agg gac atg agg cta ctg tca ttg gct gtt tcc tca gct 10027
His Lys Arg Asp Met Arg Leu Leu Ser Leu Ala Val Ser Ser Ala
3290 3295 3300
gtt ccc acc tca tgg gtt cca caa gga cgc aca aca tgg tcg att 10072
Val Pro Thr Ser Trp Val Pro Gln Gly Arg Thr Thr Trp Ser Ile
3305 3310 3315
cat ggg aaa ggg gag tgg atg acc acg gaa gac atg ctt gag gtg 10117
His Gly Lys Gly Glu Trp Met Thr Thr Glu Asp Met Leu Glu Val
3320 3325 3330
tgg aac aga gta tgg ata acc aac aac cca cac atg cag gac aag 10162
Trp Asn Arg Val Trp Ile Thr Asn Asn Pro His Met Gln Asp Lys
3335 3340 3345
aca atg gtg aaa aaa tgg aga gat gtc cct tat cta acc aag aga 10207
Thr Met Val Lys Lys Trp Arg Asp Val Pro Tyr Leu Thr Lys Arg
3350 3355 3360
caa gac aag ctg tgc gga tca ctg att gga atg acc aat agg gcc 10252
Gln Asp Lys Leu Cys Gly Ser Leu Ile Gly Met Thr Asn Arg Ala
3365 3370 3375
acc tgg gcc tcc cac atc cat tta gtc atc cat cgt atc cga acg 10297
Thr Trp Ala Ser His Ile His Leu Val Ile His Arg Ile Arg Thr
3380 3385 3390
ctg att gga cag gag aaa tac act gac tac cta aca gtc atg gac 10342
Leu Ile Gly Gln Glu Lys Tyr Thr Asp Tyr Leu Thr Val Met Asp
3395 3400 3405
agg tat tct gtg gat gct gac ctg caa ctg ggt gag ctt atc 10384
Arg Tyr Ser Val Asp Ala Asp Leu Gln Leu Gly Glu Leu Ile
3410 3415 3420
tgaaacacca tctaacagga ataaccggga tacaaaccac gggtggagaa ccggactccc 10444
cacaacctga aaccgggata taaaccacgg ctggagaacc ggactccgca cttaaaatga 10504
aacagaaacc gggataaaaa ctacggatgg agaaccggac tccacacatt gagacagaag 10564
aagttgtcag cccagaaccc cacacgagtt ttgccactgc taagctgtga ggcagtgcag 10624
gctgggacag ccgacctcca ggttgcgaaa aacctggttt ctgggacctc ccaccccaga 10684
gtaaaaagaa cggagcctcc gctaccaccc tcccacgtgg tggtagaaag acggggtcta 10744
gaggttagag gagaccctcc agggaacaaa tagtgggacc atattgacgc cagggaaaga 10804
ccggagtggt tctctgcttt tcctccagag gtctgtgagc acagtttgct casgaataag 10864
cagacctttg gatgacaaac acaaaaaccac aa 10896
<210>21
<211>3422
<212>PRT
<213>人工的
<220>
<223>合成的构建体
<400>21
Met Ser Gly Arg Lys Ala Gln Gly Lys Thr Leu Gly Val Asn Met Val
1 5 10 15
Arg Arg Gly Val Arg Ser Leu Ser Asn Lys Ile Lys Gln Lys Thr Lys
20 25 30
Gln Ile Gly Asn Arg Pro Gly Pro Ser Arg Gly Val Gln Gly Phe Ile
35 40 45
Phe Phe Phe Leu Phe Asn Ile Leu Thr Gly Lys Lys Ile Thr Ala His
50 55 60
Leu Lys Arg Leu Trp Lys Met Leu Asp Pro Arg Gln Gly Leu Ala Val
65 70 75 80
Leu Arg Lys Val Lys Arg Val Val Ala Ser Leu Met Arg Gly Leu Ser
85 90 95
Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val Gln Phe Leu Ile
100 105 110
Leu Gly Met Leu Leu Met Thr Gly Gly Val Thr Leu Ser Asn Phe Gln
115 120 125
Gly Lys Val Met Met Thr Val Asn Ala Thr Asp Val Thr Asp Val Ile
130 135 140
Thr Ile Pro Thr Ala Ala Gly Lys Asn Leu Cys Ile Val Arg Ala Met
145 150 155 160
Asp Val Gly Tyr Met Cys Asp Asp Thr Ile Thr Tyr Glu Cys Pro Val
165 170 175
Leu Ser Ala Gly Asn Asp Pro Glu Asp Ile Asp Cys Trp Cys Thr Lys
180 185 190
Ser Ala Val Tyr Val Arg Tyr Gly Arg Cys Thr Lys Thr Arg His Ser
195 200 205
Arg Arg Ser Arg Arg Ser Leu Thr Val Gln Thr His Gly Glu Ser Thr
210 215 220
Leu Ala Asn Lys Lys Gly Ala Trp Met Asp Ser Thr Lys Ala Thr Arg
225 230 235 240
Tyr Leu Val Lys Thr Glu Ser Trp Ile Leu Arg Asn Pro Gly Tyr Ala
245 250 255
Leu Val Ala Ala Val Ile Gly Trp Met Leu Gly Ser Asn Thr Met Gln
260 265 270
Arg Val Val Phe Val Val Leu Leu Leu Leu Val Ala Pro Ala Tyr Ser
275 280 285
Phe Asn Cys Leu Gly Met Ser Asn Arg Asp Phe Leu Glu Gly Val Ser
290 295 300
Gly Ala Thr Trp Val Asp Leu Val Leu Glu Gly Asp Ser Cys Val Thr
305 310 315 320
Ile Met Ser Lys Asp Lys Pro Thr Ile Asp Val Lys Met Met Asn Met
325 330 335
Glu Ala Ala Asn Leu Ala Glu Val Arg Ser Tyr Cys Tyr Leu Ala Thr
340 345 350
Val Ser Asp Leu Ser Thr Lys Ala Ala Cys Pro Thr Met Gly Glu Ala
355 360 365
His Asn Asp Lys Arg Ala Asp Pro Ala Phe Val Cys Arg Gln Gly Val
370 375 380
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Phe Phe Gly Lys Gly Ser
385 390 395 400
Ile Asp Thr Cys Ala Lys Phe Ala Cys Ser Thr Lys Ala Ile Gly Arg
405 410 415
Thr Ile Leu Lys Glu Asn Ile Lys Tyr Glu Val Ala Ile Phe Val His
420 425 430
Gly Pro Thr Thr Val Glu Ser His Gly Asn Tyr Ser Thr Gln Val Gly
435 440 445
Ala Thr Gln Ala Gly Arg Phe Ser Ile Thr Pro Ala Ala Pro Ser Tyr
450 455 460
Thr Leu Lys Leu Gly Glu Tyr Gly Glu Val Thr Val Asp Cys Glu Pro
465 470 475 480
Arg Ser Gly Ile Asp Thr Asn Ala Tyr Tyr Val Met Thr Val Gly Thr
485 490 495
Lys Thr Phe Leu Val His Arg Glu Trp Phe Met Asp Leu Asn Leu Pro
500 505 510
Trp Ser Ser Ala Gly Ser Thr Val Trp Arg Asn Arg Glu Thr Leu Met
515 520 525
Glu Phe Glu Glu Pro His Ala Thr Lys Gln Ser Val Ile Ala Leu Gly
530 535 540
Ser Gln Glu Gly Ala Leu His Gln Ala Leu Ala Gly Ala Ile Pro Val
545 550 555 560
Glu Phe Ser Ser Asn Thr Val Lys Leu Thr Ser Gly His Leu Lys Cys
565 570 575
Arg Val Lys Met Glu Lys Leu Gln Leu Lys Gly Thr Thr Tyr Gly Val
580 585 590
Cys Ser Lys Ala Phe Lys Phe Leu Arg Thr Pro Val Asp Thr Gly His
595 600 605
Gly Thr Val Val Leu Glu Leu Gln Tyr Thr Gly Thr Asp Gly Pro Cys
610 615 620
Lys Val Pro Ile Ser Ser Val Ala Ser Leu Asn Asp Leu Thr Pro Val
625 630 635 640
Gly Arg Leu Val Thr Val Asn Pro Phe Val Ser Val Ala Thr Ala Asn
645 650 655
Ala Lys Val Leu Ile Glu Leu Glu Pro Pro Phe Gly Asp Ser Tyr Ile
660 665 670
Val Val Gly Arg Gly Glu Gln Gln Ile Asn His His Trp His Lys Ser
675 680 685
Gly Ser Ser Ile Gly Lys Ala Phe Thr Thr Thr Leu Lys Gly Ala Gln
690 695 700
Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly
705 710 715 720
Gly Val Phe Thr Ser Val Gly Arg Ala Val His Gln Val Phe Gly Gly
725 730 735
Ala Phe Arg Ser Leu Phe Gly Gly Met Ser Trp Ile Thr Gln Gly Leu
740 745 750
Leu Gly Ala Leu Leu Leu Trp Met Gly Ile Asn Ala Arg Asp Arg Ser
755 760 765
Ile Ala Leu Thr Phe Leu Ala Val Gly Gly Val Leu Leu Phe Leu Ser
770 775 780
Val Asn Val Gly Ala Asp Gln Gly Cys Ala Ile Asn Phe Gly Lys Arg
785 790 795 800
Glu Leu Lys Cys Gly Asp Gly Ile Phe Ile Phe Arg Asp Ser Asp Asp
805 810 815
Trp Leu Asn Lys Tyr Ser Tyr Tyr Pro Glu Asp Pro Val Lys Leu Ala
820 825 830
Ser Ile Val Lys Ala Ser Phe Glu Glu Gly Lys Cys Gly Leu Asn Ser
835 840 845
Val Asp Ser Leu Glu His Glu Met Trp Arg Ser Arg Ala Asp Glu Ile
850 855 860
Asn Ala Ile Phe Glu Glu Asn Glu Val Asp Ile Ser Val Val Val Gln
865 870 875 880
Asp Pro Lys Asn Val Tyr Gln Arg Gly Thr His Pro Phe Ser Arg Ile
885 890 895
Arg Asp Gly Leu Gln Tyr Gly Trp Lys Thr Trp Gly Lys Asn Leu Val
900 905 910
Phe Ser Pro Gly Arg Lys Asn Gly Ser Phe Ile Ile Asp Gly Lys Ser
915 920 925
Arg Lys Glu Cys Pro Phe Ser Asn Arg Val Trp Asn Ser Phe Gln Ile
930 935 940
Glu Glu Phe Gly Thr Gly Val Phe Thr Thr Arg Val Tyr Met Asp Ala
945 950 955 960
Val Phe Glu Tyr Thr Ile Asp Cys Asp Gly Ser Ile Leu Gly Ala Ala
965 970 975
Val Asn Gly Lys Lys Ser Ala His Gly Ser Pro Thr Phe Trp Met Gly
980 985 990
Ser His Glu Val Asn Gly Thr Trp Met Ile His Thr Leu Glu Ala Leu
995 1000 1005
Asp Tyr Lys Glu Cys Glu Trp Pro Leu Thr His Thr Ile Gly Thr
1010 1015 1020
Ser Val Glu Glu Ser Glu Met Phe Met Pro Arg Ser Ile Gly Gly
1025 1030 1035
Pro Val Ser Ser His Asn His Ile Pro Gly Tyr Lys Val Gln Thr
1040 1045 1050
Asn Gly Pro Trp Met Gln Val Pro Leu Glu Val Lys Arg Glu Ala
1055 1060 1065
Cys Pro Gly Thr Ser Val Ile Ile Asp Gly Asn Cys Asp Gly Arg
1070 1075 1080
Gly Lys Ser Thr Arg Ser Thr Thr Asp Ser Gly Lys Val Ile Pro
1085 1090 1095
Glu Trp Cys Cys Arg Ser Cys Thr Met Pro Pro Val Ser Phe His
1100 1105 1110
Gly Ser Asp Gly Cys Trp Tyr Pro Met Glu Ile Arg Pro Arg Lys
1115 1120 1125
Thr His Glu Ser His Leu Val Arg Ser Trp Val Thr Ala Gly Glu
1130 1135 1140
Ile His Ala Val Pro Phe Gly Leu Val Ser Met Met Ile Ala Met
1145 1150 1155
Glu Val Val Leu Arg Lys Arg Gln Gly Pro Lys Gln Met Leu Val
1160 1165 1170
Gly Gly Val Val Leu Leu Gly Ala Met Leu Val Gly Gln Val Thr
1175 1180 1185
Leu Leu Asn Leu Leu Lys Leu Thr Val Ala Val Gly Leu His Phe
1190 1195 1200
His Glu Met Asn Asn Gly Gly Asp Ala Met Tyr Met Ala Leu Ile
1205 1210 1215
Ala Ala Phe Ser Ile Arg Pro Gly Leu Leu Ile Gly Phe Gly Leu
1220 1225 1230
Arg Thr Leu Trn Ser Pro Arg Glu Arg Leu Val Leu Thr Leu Gly
1235 1240 1245
Ala Ala Met Val Glu Ile Ala Leu Gly Gly Val Met Gly Gly Leu
1250 1255 1260
Trp Lys Tyr Leu Asn Ala Val Ser Leu Cys Ile Leu Thr Ile Asn
1265 1270 1275
Ala Val Ala Ser Arg Lys Ala Ser Asn Thr Ile Leu Pro Leu Met
1280 1285 1290
Ala Leu Leu Thr Pro Val Thr Met Ala Glu Val Arg Leu Ala Ala
1295 1300 1305
Met Phe Phe Cys Ala Met Val Ile Ile Gly Val Leu His Gln Asn
1310 1315 1320
Phe Lys Asn Thr Ser Met Gln Lys Thr Ile Pro Leu Val Ala Leu
1325 1330 1335
Thr Leu Thr Ser Tyr Leu Gly Leu Thr Gln Pro Phe Leu Gly Leu
1340 1345 1350
Cys Ala Phe Leu Ala Thr Arg Ile Phe Gly Arg Arg Ser Ile Pro
1355 1360 1365
Val Asn Glu Ala Leu Ala Ala Ala Gly Leu Val Gly Val Leu Ala
1370 1375 1380
Gly Leu Ala Phe Gln Glu Met Glu Asn Phe Leu Gly Pro Ile Ala
1385 1390 1395
Val Gly Gly Leu Leu Met Met Leu Val Ser Val Ala Gly Arg Val
1400 1405 1410
Asp Gly Leu Glu Leu Lys Lys Leu Gly Glu Val Ser Trp Glu Glu
1415 1420 1425
Glu Ala Glu Ile Ser Gly Ser Ser Ala Arg Tyr Asp Val Ala Leu
1430 1435 1440
Ser Glu Gln Gly Glu Phe Lys Leu Leu Ser Glu Glu Lys Val Pro
1445 1450 1455
Trp Asp Gln Val Val Met Thr Ser Leu Ala Leu Val Gly Ala Ala
1460 1465 1470
Leu His Pro Phe Ala Leu Leu Leu Val Leu Ala Gly Trp Leu Phe
1475 1480 1485
His Val Arg Gly Ala Arg Arg Ser Gly Asp Val Leu Trp Asp Ile
1490 1495 1500
Pro Thr Pro Lys Ile Ile Glu Glu Cys Glu His Leu Glu Asp Gly
1505 1510 1515
Ile Tyr Gly Ile Phe Gln Ser Thr Phe Leu Gly Ala Ser Gln Arg
1520 1525 1530
Gly Val Gly Val Ala Gln Gly Gly Val Phe His Thr Met Trp His
1535 1540 1545
Val Thr Arg Gly Ala Phe Leu Val Arg Asn Gly Lys Lys Leu Ile
1550 1555 1560
Pro Ser Trp Ala Ser Val Lys Glu Asp Leu Val Ala Tyr Gly Gly
1565 1570 1575
Ser Trp Lys Leu Glu Gly Arg Trp Asp Gly Glu Glu Glu Val Gln
1580 1585 1590
Leu Ile Ala Ala Val Pro Gly Lys Asn Val Val Asn Val Gln Thr
1595 1600 1605
Lys Pro Ser Leu Phe Lys Val Arg Asn Gly Gly Glu Ile Gly Ala
1610 1615 1620
Val Ala Leu Asp Tyr Pro Ser Gly Thr Ser Gly Ser Pro Ile Val
1625 1630 1635
Asn Arg Asn Gly Glu Val Ile Gly Leu Tyr Gly Asn Gly Ile Leu
1640 1645 1650
Val Gly Asp Asn Ser Phe Val Ser Ala Ile Ser Gln Thr Glu Val
1655 1660 1665
Lys Glu Glu Gly Lys Glu Glu Leu Gln Glu Ile Pro Thr Met Leu
1670 1675 1680
Lys Lys Gly Met Thr Thr Val Leu Asp Phe His Pro Gly Ala Gly
1685 1690 1695
Lys Thr Arg Arg Phe Leu Pro Gln Ile Leu Ala Glu Cys Ala Arg
1700 1705 1710
Arg Arg Leu Arg Thr Leu Val Leu Ala Pro Thr Arg Val Val Leu
1715 1720 1725
Ser Glu Met Lys Glu Ala Phe His Gly Leu Asp Val Lys Phe His
1730 1735 1740
Thr Gln Ala Phe Ser Ala His Gly Ser Gly Arg Glu Val Ile Asp
1745 1750 1755
Ala Met Cys His Ala Thr Leu Thr Tyr Arg Met Leu Glu Pro Thr
1760 1765 1770
Arg Val Val Asn Trp Glu Val Ile Ile Met Asp Glu Ala His Phe
1775 1780 1785
Leu Asp Pro Ala Ser Ile Ala Ala Arg Gly Trp Ala Ala His Arg
1790 1795 1800
Ala Arg Ala Asn Glu Ser Ala Thr Ile Leu Met Thr Ala Thr Pro
1805 1810 1815
Pro Gly Thr Ser Asp Glu Phe Pro His Ser Asn Gly Glu Ile Glu
1820 1825 1830
Asp Val Gln Thr Asp Ile Pro Ser Glu Pro Trp Asn Thr Gly His
1835 1840 1845
Asp Trp Ile Leu Ala Asp Lys Arg Pro Thr Ala Trp Phe Leu Pro
1850 1855 1860
Ser Ile Arg Ala Ala Asn Val Met Ala Ala Ser Leu Arg Lys Ala
1865 1870 1875
Gly Lys Ser Val Val Val Leu Asn Arg Lys Thr Phe Glu Arg Glu
1880 1885 1890
Tyr Pro Thr Ile Lys Gln Lys Lys Pro Asp Phe Ile Leu Ala Thr
1895 1900 1905
Asp Ile Ala Glu Met Gly Ala Asn Leu Cys Val Glu Arg Val Leu
1910 1915 1920
Asp Cys Arg Thr Ala Phe Lys Pro Val Leu Val Asp Glu Gly Arg
1925 1930 1935
Lys Val Ala Ile Lys Gly Pro Leu Arg Ile Ser Ala Ser Ser Ala
1940 1945 1950
Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro Asn Arg Asp Gly
1955 1960 1965
Asp Ser Tyr Tyr Tyr Ser Glu Pro Thr Ser Glu Asn Asn Ala His
1970 1975 1980
His Val Cys Trp Leu Glu Ala Ser Met Leu Leu Asp Asn Met Glu
1985 1990 1995
Val Arg Gly Gly Met Val Ala Pro Leu Tyr Gly Val Glu Gly Thr
2000 2005 2010
Lys Thr Pro Val Ser Pro Gly Glu Met Arg Leu Arg Asp Asp Gln
2015 2020 2025
Arg Lys Val Phe Arg Glu Leu Val Arg Asn Cys Asp Leu Pro Val
2030 2035 2040
Trp Leu Ser Trp Gln Val Ala Lys Ala Gly Leu Lys Thr Asn Asp
2045 2050 2055
Arg Lys Trp Cys Phe Glu Gly Pro Glu Glu His Glu Ile Leu Asn
2060 2065 2070
Asp Ser Gly Glu Thr Val Lys Cys Arg Ala Pro Gly Gly Ala Lys
2075 2080 2085
Lys Pro Leu Arg Pro Arg Trp Cys Asp Glu Arg Val Ser Ser Asp
2090 2095 2100
Gln Ser Ala Leu Ser Glu Phe Ile Lys Phe Ala Glu Gly Arg Arg
2105 2110 2115
Gly Ala Ala Glu Val Leu Val Val Leu Ser Glu Leu Pro Asp Phe
2120 2125 2130
Leu Ala Lys Lys Gly Gly Glu Ala Met Asp Thr Ile Ser Val Phe
2135 2140 2145
Leu His Ser Glu Glu Gly Ser Arg Ala Tyr Arg Asn Ala Leu Ser
2150 2155 2160
Met Met Pro Glu Ala Met Thr Ile Val Met Leu Phe Ile Leu Ala
2165 2170 2175
Gly Leu Leu Thr Ser Gly Met Val Ile Phe Phe Met Ser Pro Lys
2180 2185 2190
Gly Ile Ser Arg Met Ser Met Ala Met Gly Thr Met Ala Gly Cys
2195 2200 2205
Gly Tyr Leu Met Phe Leu Gly Gly Val Lys Pro Thr His Ile Ser
2210 2215 2220
Tyr Val Met Leu Ile Phe Phe Val Leu Met Val Val Val Ile Pro
2225 2230 2235
Glu Pro Gly Gln Gln Arg Ser Ile Gln Asp Asn Gln Val Ala Tyr
2240 2245 2250
Leu Ile Ile Gly Ile Leu Thr Leu Val Ser Ala Val Ala Ala Asn
2255 2260 2265
Glu Leu Gly Met Leu Glu Lys Thr Lys Glu Asp Leu Phe Gly Lys
2270 2275 2280
Lys Asn Leu Ile Pro Ser Ser Ala Ser Pro Trp Ser Trp Pro Asp
2285 2290 2295
Leu Asp Leu Lys Pro Gly Ala Ala Trp Thr Val Tyr Val Gly Ile
2300 2305 2310
Val Thr Met Leu Ser Pro Met Leu His His Trp Ile Lys Val Glu
2315 2320 2325
Tyr Gly Asn Leu Ser Leu Ser Gly Ile Ala Gln Ser Ala Ser Val
2330 2335 2340
Leu Ser Phe Met Asp Lys Gly Ile Pro Phe Met Lys Met Asn Ile
2345 2350 2355
Ser Val Ile Met Leu Leu Val Ser Gly Trp Asn Ser Ile Thr Val
2360 2365 2370
Met Pro Leu Leu Cys Gly Ile Gly Cys Ala Met Leu His Trp Ser
2375 2380 2385
Leu Ile Leu Pro Gly Ile Lys Ala Gln Gln Ser Lys Leu Ala Gln
2390 2395 2400
Arg Arg Val Phe His Gly Val Ala Lys Asn Pro Val Val Asp Gly
2405 2410 2415
Asn Pro Thr Val Asp Ile Glu Glu Ala Pro Glu Met Pro Ala Leu
2420 2425 2430
Tyr Glu Lys Lys Leu Ala Leu Tyr Leu Leu Leu Ala Leu Ser Leu
2435 2440 2445
Ala Ser Val Ala Met Cys Arg Thr Pro Phe Ser Leu Ala Glu Gly
2450 2455 2460
Ile Val Leu Ala Ser Ala Ala Leu Gly Pro Leu Ile Glu Gly Asn
2465 2470 2475
Thr Ser Leu Leu Trp Asn Gly Pro Met Ala Val Ser Met Thr Gly
2480 2485 2490
Val Met Arg Gly Asn His Tyr Ala Phe Val Gly Val Met Tyr Asn
2495 2500 2505
Leu Trp Lys Met Lys Thr Gly Arg Arg Gly Ser Ala Asn Gly Lys
2510 2515 2520
Thr Leu Gly Glu Val Trp Lys Arg Glu Leu Asn Leu Leu Asp Lys
2525 2530 2535
Arg Gln Phe Glu Leu Tyr Lys Arg Thr Asp Ile Val Glu Val Asp
2540 2545 2550
Arg Asp Thr Ala Arg Arg His Leu Ala Glu Gly Lys Val Asp Thr
2555 2560 2565
Gly Val Ala Val Ser Arg Gly Thr Ala Lys Leu Arg Trp Phe His
2570 2575 2580
Glu Arg Gly Tyr Val Lys Leu Glu Gly Arg Val Ile Asp Leu Gly
2585 2590 2595
Cys Gly Arg Gly Gly Trp Cys Tyr Tyr Ala Ala Ala Gln Lys Glu
2600 2605 2610
Val Ser Gly Val Lys Gly Phe Thr Leu Gly Arg Asp Gly His Glu
2615 2620 2625
Lys Pro Met Asn Val Gln Ser Leu Gly Trn Asn Ile Ile Thr Phe
2630 2635 2640
Lys Asp Lys Thr Asp Ile His Arg Leu Glu Pro Val Lys Cys Asp
2645 2650 2655
Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Ser Ser Val Thr
2660 2665 2670
Glu Gly Glu Arg Thr Val Arg Val Leu Asp Thr Val Glu Lys Trp
2675 2680 2685
Leu Ala Cys Gly Val Asp Asn Phe Cys Val Lys Val Leu Ala Pro
2690 2695 2700
Tyr Met Pro Asp Val Leu Glu Lys Leu Glu Leu Leu Gln Arg Arg
2705 2710 2715
Phe Gly Gly Thr Val Ile Arg Asn Pro Leu Ser Arg Asn Ser Thr
2720 2725 2730
His Glu Met Tyr Tyr Val Ser Gly Ala Arg Ser Asn Val Thr Phe
2735 2740 2745
Thr Val Asn Gln Thr Ser Arg Leu Leu Met Arg Arg Met Arg Arg
2750 2755 2760
Pro Thr Gly Lys Val Thr Leu Glu Ala Asp Val Ile Leu Pro Ile
2765 2770 2775
Gly Thr Arg Ser Val Glu Thr Asn Lys Gly Pro Leu Asn Lys Glu
2780 2785 2790
Ala Ile Glu Glu Arg Val Glu Arg Lle Lys Ser Glu Tyr Met Thr
2795 2800 2805
Ser Trp Phe Tyr Asp Asn Asp Asn Pro Tyr Arg Thr Trp His Tyr
2810 2815 2820
Cys Gly Ser Tyr Val Thr Lys Thr Ser Gly Ser Ala Ala Ser Met
2825 2830 2835
Val Asn Gly Val Ile Lys Ile Leu Thr Tyr Pro Trn Asn Arg Ile
2840 2845 2850
Glu Glu Val Thr Arg Met Ala Met Thr Asp Thr Thr Pro Phe Gly
2855 2860 2865
Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Ala Lys Asp
2870 2875 2880
Pro Pro Ala Gly Thr Arg Lys Ile Met Lys Val Val Asn Arg Trp
2885 2890 2895
Leu Phe Arg His Leu Ala Arg Glu Lys Asn Pro Arg Leu Cys Thr
2900 2905 2910
Lys Glu Glu Phe Ile Ala Lys Val Arg Ser His Ala Ala Ile Gly
2915 2920 2925
Ala Tyr Leu Glu Glu Gln Glu Gln Trp Lys Thr Ala Asn Glu Ala
2930 2935 2940
Val Gln Asp Pro Lys Phe Trp Glu Leu Val Asp Glu Glu Arg Lys
2945 2950 2955
Leu His Gln Gln Gly Arg Cys Arg Thr Cys Val Tyr Asn Met Met
2960 2965 2970
Gly Lys Arg Glu Lys Lys Leu Ser Glu Phe Gly Lys Ala Lys Gly
2975 2980 2985
Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Tyr Leu Glu
2990 2995 3000
Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Ala Ser Arg
3005 3010 3015
Glu Asn Ser Gly Gly Gly Val Glu Gly Ile Gly Leu Gln Tyr Leu
3020 3025 3030
Gly Tyr Val Ile Arg Asp Leu Ala Ala Met Asp Gly Gly Gly Phe
3035 3040 3045
Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu Ala
3050 3055 3060
Asp Leu Asp Asp Glu Gln Glu Ile Leu Asn Tyr Met Ser Pro His
3065 3070 3075
His Lys Lys Leu Ala Gln Ala Val Met Glu Met Thr Tyr Lys Asn
3080 3085 3090
Lys Val Val Lys Val Leu Arg Pro Ala Pro Gly Gly Lys Ala Tyr
3095 3100 3105
Met Asp Val Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly Gln Val
3110 3115 3120
Val Thr Tyr Ala Leu Asn Thr Ile Thr Asn Leu Lys Val Gln Leu
3125 3130 3135
Ile Arg Met Ala Glu Ala Glu Met Val Ile His His Gln His Val
3140 3145 3150
Gln Asp Cys Asp Glu Ser Val Leu Thr Arg Leu Glu Ala Trp Leu
3155 3160 3165
Thr Glu His Gly Cys Asp Arg Leu Lys Arg Met Ala Val Ser Gly
3170 3175 3180
Asp Asp Cys Val Val Arg Pro Ile Asp Asp Arg Phe Gly Leu Ala
3185 3190 3195
Leu Ser His Leu Asn Ala Met Ser Lys Val Arg Lys Asp Ile Ser
3200 3205 3210
Glu Trp Gln Pro Ser Lys Gly Trp Asn Asp Trp Glu Asn Val Pro
3215 3220 3225
Phe Cys Ser His His Phe His Glu Leu Gln Leu Lys Asp Gly Arg
3230 3235 3240
Arg Ile Val Val Pro Cys Arg Glu Gln Asn Glu Leu Ile Gly Arg
3245 3250 3255
Gly Arg Val Ser Pro Gly Asn Gly Trp Met Ile Lys Glu Thr Ala
3260 3265 3270
Cys Leu Ser Lys Ala Tyr Ala Asn Met Trp Ser Leu Met Tyr Phe
3275 3280 3285
His Lys Arg Asp Met Arg Leu Leu Ser Leu Ala Val Ser Ser Ala
3290 3295 3300
Val Pro Thr Ser Trp Val Pro Gln Gly Arg Thr Thr Trp Ser Ile
3305 3310 3315
His Gly Lys Gly Glu Trp Met Thr Thr Glu Asp Met Leu Glu Val
3320 3325 3330
Trp Asn Arg Val Trp Ile Thr Asn Asn Pro His Met Gln Asp Lys
3335 3340 3345
Thr Met Val Lys Lys Trp Arg Asp Val Pro Tyr Leu Thr Lys Arg
3350 3355 3360
Gln Asp Lys Leu Cys Gly Ser Leu Ile Gly Met Thr Asn Arg Ala
3365 3370 3375
Thr Trp Ala Ser His Ile His Leu Val Ile His Arg Ile Arg Thr
3380 3385 3390
Leu Ile Gly Gln Glu Lys Tyr Thr Asp Tyr Leu Thr Val Met Asp
3395 3400 3405
Arg Tyr Ser Val Asp Ala Asp Leu Gln Leu Gly Glu Leu Ile
3410 3415 3420
<210>22
<211>10896
<212>DNA
<213>人工的
<220>
<223>衍生于黄热病病毒和西尼罗病毒
<220>
<221>misc_feature
<222>(1)..(1)
<223>n是a,c,g,或t
<220>
<221>CDS
<222>(119)..(10384)
<400>22
ngtaaatcct gtgtgctaat tgaggtgcat tggtctgcaa atcgagttgc taggcaataa 60
acacatttgg attaatttta atcgttcgtt gagcgattag cagagaactg accagaac 118
atg tct ggt cgt aaa gct cag gga aaa acc ctg ggc gtc aat atg gta 166
Met Ser Gly Arg Lys Ala Gln Gly Lys Thr Leu Gly Val Asn Met Val
1 5 10 15
cga cga gga gtt cgc tcc ttg tca aac aaa ata aaa caa aaa aea aaa 214
Arg Arg Gly Val Arg Ser Leu Ser Asn Lys Ile Lys Gln Lys Thr Lys
20 25 30
caa att gga aac aga cct gga cct tca aga ggt gtt caa gga ttt atc 262
Gln Ile Gly Asn Arg Pro Gly Pro Ser Arg Gly Val Gln Gly Phe Ile
35 40 45
ttt ttc ttt ttg ttc aac att ttg act gga aaa aag atc aca gcc cac 310
Phe Phe Phe Leu Phe Asn Ile Leu Thr Gly Lys Lys Ile Thr Ala His
50 55 60
cta aag agg ttg tgg aaa atg ctg gac cca aga caa ggc ttg gct gtt 358
Leu Lys Arg Leu Trp Lys Met Leu Asp Pro Arg Gln Gly Leu Ala Val
65 70 75 80
cta agg aaa gtc aag aga gtg gtg gcc agt ttg atg aga gga ttg tcc 406
Leu Arg Lys Val Lys Arg Val Val Ala Ser Leu Met Arg Gly Leu Ser
85 90 95
tca agg aaa cgc cgt tcc cat gat gtt ctg act gtg caa ttc cta att 454
Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val Gln Phe Leu Ile
100 105 110
ttg gga atg ctg ttg atg acg ggt gga gtt acc ctc tct aac ttc caa 502
Leu Gly Met Leu Leu Met Thr Gly Gly Val Thr Leu Ser Asn Phe Gln
115 120 125
ggg aag gtg atg atg acg gta aat gct act gac gtc aca gat gtc atc 550
Gly Lys Val Met Met Thr Val Asn Ala Thr Asp Val Thr Asp Val Ile
130 135 140
acg att cca aca gct gct gga aag aac cta tgc att gtc aga gca atg 598
Thr Ile Pro Thr Ala Ala Gly Lys Asn Leu Cys Ile Val Arg Ala Met
145 150 155 160
gat gtg gga tac atg tgc gat gat act atc act tat gaa tgc cca gtg 646
Asp Val Gly Tyr Met Cys Asp Asp Thr Ile Thr Tyr Glu Cys Pro Val
165 170 175
ctg tcg gct ggt aat gat cca gaa gac atc gac tgt tgg tgc aca aag 694
Leu Ser Ala Gly Asn Asp Pro Glu Asp Ile Asp Cys Trp Cys Thr Lys
180 185 190
tca gca gtc tac gtc agg tat gga aga tgc acc aag aca cgc cac tca 742
Ser Ala Val Tyr Val Arg Tyr Gly Arg Cys Thr Lys Thr Arg His Ser
195 200 205
aga cgc agt cgg agg tca ctg aca gtg cag aca cac gga gaa agc act 790
Arg Arg Ser Arg Arg Ser Leu Thr Val Gln Thr His Gly Glu Ser Thr
210 215 220
cta gcg aac aag aag ggg gct tgg atg gac agc acc aag gcc aca agg 838
Leu Ala Asn Lys Lys Gly Ala Trp Met Asp Ser Thr Lys Ala Thr Arg
225 230 235 240
tat ttg gta aaa aca gaa tca tgg atc ttg agg aac cct gga tat gcc 886
Tyr Leu Val Lys Thr Glu Ser Trp Ile Leu Arg Asn Pro Gly Tyr Ala
245 250 255
ctg gtg gca gcc gtc att ggt tgg atg ctt ggg agc aac acc atg cag 934
Leu Val Ala Ala Val Ile Gly Trp Met Leu Gly Ser Asn Thr Met Gln
260 265 270
aga gtt gtg ttt gtc gtg cca ttg ctt ttg gtg gcc cca gct tac agc 982
Arg Val Val Phe Val Val Pro Leu Leu Leu Val Ala Pro Ala Tyr Ser
275 280 285
ttc aac tgc ctt gga atg agc aac aga gac ttc ttg gaa gga gtg tct 1030
Phe Asn Cys Leu Gly Met Ser Asn Arg Asp Phe Leu Glu Gly Val Ser
290 295 300
gga gca aca tgg gtg gat ttg gtt ctc gaa ggc gac agc tgc gtg act 1078
Gly Ala Thr Trp Val Asp Leu Val Leu Glu Gly Asp Ser Cys Val Thr
305 310 315 320
atc atg tct aag gac aag cct acc atc gac gtc aag atg atg aat atg 1126
Ile Met Ser Lys Asp Lys Pro Thr Ile Asp Val Lys Met Met Asn Met
325 330 335
gag gcg gcc aac ctg gca gag gtc cgc agt tat tgc tat ttg gct acc 1174
Glu Ala Ala Asn Leu Ala Glu Val Arg Ser Tyr Cys Tyr Leu Ala Thr
340 345 350
gtc agc gat ctc tcc acc aaa gct gca tgc ccg acc atg gga gaa gct 1222
Val Ser Asp Leu Ser Thr Lys Ala Ala Cys Pro Thr Met Gly Glu Ala
355 360 365
cac aat gac aaa cgt gct gac cca gct ttt gtg tgc aga caa gga gtg 1270
His Asn Asp Lys Arg Ala Asp Pro Ala Phe Val Cys Arg Gln Gly Val
370 375 380
gtg gac agg ggc tgg ggc aac ggc tgc gga ttt ttt ggc aaa gga tcc 1318
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Phe Phe Gly Lys Gly Ser
385 390 395 400
att gac aca tgc gcc aaa ttt gcc tgc tct acc aag gca ata gga aga 1366
Ile Asp Thr Cys Ala Lys Phe Ala Cys Ser Thr Lys Ala Ile Gly Arg
405 410 415
acc atc ttg aaa gag aat atc aag tac gaa gtg gcc att ttt gtc cat 1414
Thr Ile Leu Lys Glu Asn Ile Lys Tyr Glu Val Ala Ile Phe Val His
420 425 430
gga cca act act gtg gag tcg cac gga aat tac tcc aca cag gtt gga 1462
Gly Pro Thr Thr Val Glu Ser His Gly Asn Tyr Ser Thr Gln Val Gly
435 440 445
gcc act cag gcc ggc cga ttc agc atc act cct gct gcg cct tca tac 1510
Ala Thr Gln Ala Gly Arg Phe Ser Ile Thr Pro Ala Ala Pro Ser Tyr
450 455 460
aca cta aag ctt gga gaa tat gga gag gtg aca gtg gac tgt gaa cca 1558
Thr Leu Lys Leu Gly Glu Tyr Gly Glu Val Thr Val Asp Cys Glu Pro
465 470 475 480
cgg tca ggg att gac acc aat gca tac tac gtg atg act gtt gga aca 1606
Arg Ser Gly Ile Asp Thr Asn Ala Tyr Tyr Val Met Thr Val Gly Thr
485 490 495
aag acg ttc ttg gtc cat cgt gag tgg ttc atg gac ctc aac ctc cct 1654
Lys Thr Phe Leu Val His Arg Glu Trp Phe Met Asp Leu Asn Leu Pro
500 505 510
tgg agc agt gct gga agt act gtg tgg agg aac aga gag acg tta atg 1702
Trp Ser Ser Ala Gly Ser Thr Val Trp Arg Asn Arg Glu Thr Leu Met
515 520 525
gag ttt gag gaa cca cac gcc acg aag cag tct gtg ata gca ttg ggc 1750
Glu Phe Glu Glu Pro His Ala Thr Lys Gln Ser Val Ile Ala Leu Gly
530 535 540
tca caa gag gga gct ctg cat caa gct ttg gct gga gcc att cct gtg 1798
Ser Gln Glu Gly Ala Leu His Gln Ala Leu Ala Gly Ala Ile Pro Val
545 550 555 560
gaa ttt tca agc aac act gtc aag ttg acg tcg ggt cat ttg aag tgt 1846
Glu Phe Ser Ser Asn Thr Val Lys Leu Thr Ser Gly His Leu Lys Cys
565 570 575
aga gtg aag atg gaa aaa ttg cag ttg aag gga aca acc tat ggc gtc 1894
Arg Val Lys Met Glu Lys Leu Gln Leu Lys Gly Thr Thr Tyr Gly Val
580 585 590
tgt tca aag gct ttc aag ttt ctt agg act ccc gtg gac acc ggt cac 1942
Cys Ser Lys Ala Phe Lys Phe Leu Arg Thr Pro Val Asp Thr Gly His
595 600 605
ggc act gtg gtg ttg gaa ttg cag tac act ggc acg gat gga cct tgc 1990
Gly Thr Val Val Leu Glu Leu Gln Tyr Thr Gly Thr Asp Gly Pro Cys
610 615 620
aaa gtt cct atc tcg tca gtg gct tca ttg aac gac cta acg cca gtg 2038
Lys Val Pro Ile Ser Ser Val Ala Ser Leu Asn Asp Leu Thr Pro Val
625 630 635 640
ggc aga ttg gtc act gtc aac cct ttt gtt tca gtg gcc acg gcc aac 2086
Gly Arg Leu Val Thr Val Asn Pro Phe Val Ser Val Ala Thr Ala Asn
645 650 655
gct aag gtc ctg att gaa ttg gaa cca ccc ttt gga gac tca tac ata 2134
Ala Lys Val Leu Ile Glu Leu Glu Pro Pro Phe Gly Asp Ser Tyr Ile
660 665 670
gtg gtg ggc aga gga gaa caa cag atc aat cac cat tgg cac aag tct 2182
Val Val Gly Arg Gly Glu Gln Gln Ile Asn His His Trp His Lys Ser
675 680 685
gga agc agc att ggc aaa gcc ttt aca acc acc ctc aaa gga gcg cag 2230
Gly Ser Ser Ile Gly Lys Ala Phe Thr Thr Thr Leu Lys Gly Ala Gln
690 695 700
aga cta gcc gct cta gga gac aca gct tgg gac ttt gga tca gtt gga 2278
Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly
705 710 715 720
ggg gtg ttc act agt gtt ggg cgg gct gtc cat caa gtg ttc gga gga 2326
Gly Val Phe Thr Ser Val Gly Arg Ala Val His Gln Val Phe Gly Gly
725 730 735
gca ttc cgc tca ctg ttc gga ggc atg tcc tgg ata acg caa gga ttg 2374
Ala Phe Arg Ser Leu Phe Gly Gly Met Ser Trp Ile Thr Gln Gly Leu
740 745 750
ctg ggg gct ctc ctg ttg tgg atg ggc atc aat gct cgt gat agg tcc 2422
Leu Gly Ala Leu Leu Leu Trp Met Gly Ile Asn Ala Arg Asp Arg Ser
755 760 765
ata gct ctc acg ttt ctc gca gtt gga gga gtt ctg ctc ttc ctc tcc 2470
Ile Ala Leu Thr Phe Leu Ala Val Gly Gly Val Leu Leu Phe Leu Ser
770 775 780
gtg aac gtg ggc gcc gat caa gga tgc gcc atc aac ttt ggc aag aga 2518
Val Asn Val Gly Ala Asp Gln Gly Cys Ala Ile Asn Phe Gly Lys Arg
785 790 795 800
gag ctc aag tgc gga gat ggt atc ttc ata ttt aga gac tct gat gac 2566
Glu Leu Lys Cys Gly Asp Gly Ile Phe Ile Phe Arg Asp Ser Asp Asp
805 810 815
tgg ctg aac aag tac tca tac tat cca gaa gat cct gtg aag ctt gca 2614
Trp Leu Asn Lys Tyr Ser Tyr Tyr Pro Glu Asp Pro Val Lys Leu Ala
820 825 830
tca ata gtg aaa gcc tct ttt gaa gaa ggg aag tgt ggc cta aat tca 2662
Ser Ile Val Lys Ala Ser Phe Glu Glu Gly Lys Cys Gly Leu Asn Ser
835 840 845
gtt gac tcc ctt gag cat gag atg tgg aga agc agg gca gat gag atc 2710
Val Asp Ser Leu Glu His Glu Met Trp Arg Ser Arg Ala Asp Glu Ile
850 855 860
aat gcc att ttt gag gaa aac gag gtg gac att tct gtt gtc gtg cag 2758
Asn Ala Ile Phe Glu Glu Asn Glu Val Asp Ile Ser Val Val Val Gln
865 870 875 880
gat cca aag aat gtt tac cag aga gga act cat cca ttt tcc aga att 2806
Asp Pro Lys Asn Val Tyr Gln Arg Gly Thr His Pro Phe Ser Arg Ile
885 890 895
cgg gat ggt ctg cag tat ggt tgg aag act tgg ggt aag aac ctt gtg 2854
Arg Asp Gly Leu Gln Tyr Gly Trp Lys Thr Trp Gly Lys Asn Leu Val
900 905 910
ttc tcc cca ggg agg aag aat gga agc ttc atc ata gat gga aag tcc 2902
Phe Ser Pro Gly Arg Lys Asn Gly Ser Phe Ile Ile Asp Gly Lys Ser
915 920 925
agg aaa gaa tgc ccg ttt tca aac cgg gtc tgg aat tct ttc cag ata 2950
Arg Lys Glu Cys Pro Phe Ser Asn Arg Val Trp Asn Ser Phe Gln Ile
930 935 940
gag gag ttt ggg acg gga gtg ttc acc aca cgc gtg tac atg gac gca 2998
Glu Glu Phe Gly Thr Gly Val Phe Thr Thr Arg Val Tyr Met Asp Ala
945 950 955 960
gtc ttt gaa tac acc ata gac tgc gat gga tct atc ttg ggt gca gcg 3046
Val Phe Glu Tyr Thr Ile Asp Cys Asp Gly Ser Ile Leu Gly Ala Ala
965 970 975
gtg aac gga aaa aag agt gcc cat ggc tct cca aca ttt tgg atg gga 3094
Val Asn Gly Lys Lys Ser Ala His Gly Ser Pro Thr Phe Trp Met Gly
980 985 990
agt cat gaa gta aat ggg aca tgg atg atc cac acc ttg gag gca tta 3142
Ser His Glu Val Asn Gly Thr Trp Met Ile His Thr Leu Glu Ala Leu
995 1000 1005
gat tac aag gag tgt gag tgg cca ctg aca cat acg att gga aca 3187
Asp Tyr Lys Glu Cys Glu Trp Pro Leu Thr His Thr Ile Gly Thr
1010 1015 1020
tca gtt gaa gag agt gaa atg ttc atg ccg aga tca atc gga ggc 3232
Ser Val Glu Glu Ser Glu Met Phe Met Pro Arg Ser Ile Gly Gly
1025 1030 1035
cca gtt agc tct cac aat cat atc cct gga tac aag gtt cag acg 3277
Pro Val Ser Ser His Asn His Ile Pro Gly Tyr Lys Val Gln Thr
1040 1045 1050
aac gga cct tgg atg cag gta cca cta gaa gtg aag aga gaa gct 3322
Asn Gly Pro Trp Met Gln Val Pro Leu Glu Val Lys Arg Glu Ala
1055 1060 1065
tgc cca ggg act agc gtg atc att gat ggc aac tgt gat gga cgg 3367
Cys Pro Gly Thr Ser Val Ile Ile Asp Gly Asn Cys Asp Gly Arg
1070 1075 1080
gga aaa tca acc aga tcc acc acg gat agc ggg aaa gtt att cct 3412
Gly Lys Ser Thr Arg Ser Thr Thr Asp Ser Gly Lys Val Ile Pro
1085 1090 1095
gaa tgg tgt tgc cgc tcc tgc aca atg ccg cct gtg agc ttc cat 3457
Glu Trp Cys Cys Arg Ser Cys Thr Met Pro Pro Val Ser Phe His
1100 1105 1110
ggt agt gat ggg tgt tgg tat ccc atg gaa att agg cca agg aaa 3502
Gly Ser Asp Gly Cys Trp Tyr Pro Met Glu Ile Arg Pro Arg Lys
1115 1120 1125
acg cat gaa agc cat ctg gtg cgc tcc tgg gtt aca gct gga gaa 3547
Thr His Glu Ser His Leu Val Arg Ser Trp Val Thr Ala Gly Glu
1130 1135 1140
ata cat gct gtc cct ttt ggt ttg gtg agc atg atg ata gca atg 3592
Ile His Ala Val Pro Phe Gly Leu Val Ser Met Met Ile Ala Met
1145 1150 1155
gaa gtg gtc cta agg aaa aga cag gga cca aag caa atg ttg gtt 3637
Glu Val Val Leu Arg Lys Arg Gln Gly Pro Lys Gln Met Leu Val
1160 1165 1170
gga gga gta gtg ctc ttg gga gca atg ctg gtc ggg caa gta act 3682
Gly Gly Val Val Leu Leu Gly Ala Met Leu Val Gly Gln Val Thr
1175 1180 1185
ctc ctt gat ttg ctg aaa ctc aca gtg gct gtg gga ttg cat ttc 3727
Leu Leu Asp Leu Leu Lys Leu Thr Val Ala Val Gly Leu His Phe
1190 1195 1200
cat gag atg aac aat gga gga gac gcc atg tat atg gcg ttg att 3772
His Glu Met Asn Asn Gly Gly Asp Ala Met Tyr Met Ala Leu Ile
1205 1210 1215
gct gcc ttt tca atc aga cca ggg ctg ctc atc ggc ttt ggg ctc 3817
Ala Ala Phe Ser Ile Arg Pro Gly Leu Leu Ile Gly Phe Gly Leu
1220 1225 1230
agg acc cta tgg agc cct cgg gaa cgc ctt gtg ctg acc cta gga 3862
Arg Thr Leu Trp Ser Pro Arg Glu Arg Leu Val Leu Thr Leu Gly
1235 1240 1245
gca gcc atg gtg gag att gcc ttg ggt ggc gtg atg ggc ggc ctg 3907
Ala Ala Met Val Glu Ile Ala Leu Gly Gly Val Met Gly Gly Leu
1250 1255 1260
tgg aag tat cta aat gca gtt tct ctc tgc atc ctg aca ata aat 3952
Trp Lys Tyr Leu Asn Ala Val Ser Leu Cys Ile Leu Thr Ile Asn
1265 1270 1275
gct gtt gct tct agg aaa gca tca aat acc atc ttg ccc ctc atg 3997
Ala Val Ala Ser Arg Lys Ala Ser Asn Thr Ile Leu Pro Leu Met
1280 1285 1290
gct ctg ttg aca cct gtc act atg gct gag gtg aga ctt gcc gca 4042
Ala Leu Leu Thr Pro Val Thr Met Ala Glu Val Arg Leu Ala Ala
1295 1300 1305
atg ttc ttt tgt gcc atg gtt atc ata ggg gtc ctt cac cag aat 4087
Met Phe Phe Cys Ala Met Val Ile Ile Gly Val Leu His Gln Asn
1310 1315 1320
ttc aag gac acc tcc atg cag aag act ata cct ctg gtg gcc ctc 4132
Phe Lys Asp Thr Ser Met Gln Lys Thr Ile Pro Leu Val Ala Leu
1325 1330 1335
aca ctc aca tct tac ctg ggc ttg aca caa cct ttt ttg ggc ctg 4177
Thr Leu Thr Ser Tyr Leu Gly Leu Thr Gln Pro Phe Leu Gly Leu
1340 1345 1350
tgt gca ttt ctg gca acc cgc ata ttt ggg cga agg agt atc cca 4222
Cys Ala Phe Leu Ala Thr Arg Ile Phe Gly Arg Arg Ser Ile Pro
1355 1360 1365
gtg aat gag gca ctc gca gca gct ggt cta gtg gga gtg ctg gca 4267
Val Asn Glu Ala Leu Ala Ala Ala Gly Leu Val Gly Val Leu Ala
1370 1375 1380
gga ctg gct ttt cag gag atg gag aac ttc ctt ggt ccg att gca 4312
Gly Leu Ala Phe Gln Glu Met Glu Asn Phe Leu Gly Pro Ile Ala
1385 1390 1395
gtt gga gga ctc ctg atg atg ctg gtt agc gtg gct ggg agg gtg 4357
Val Gly Gly Leu Leu Met Met Leu Val Ser Val Ala Gly Arg Val
1400 1405 1410
gat ggg cta gag ctc aag aag ctt ggt gaa gtt tca tgg gaa gag 4402
Asp Gly Leu Glu Leu Lys Lys Leu Gly Glu Val Ser Trp Glu Glu
1415 1420 1425
gag gcg gag atc agc ggg agt tcc gcc cgc tat gat gtg gca ctc 4447
Glu Ala Glu Ile Ser Gly Ser Ser Ala Arg Tyr Asp Val Ala Leu
1430 1435 1440
agt gaa caa ggg gag ttc aag ctg ctt tct gaa gag aaa gtg cca 4492
Ser Glu Gln Gly Glu Phe Lys Leu Leu Ser Glu Glu Lys Val Pro
1445 1450 1455
tgg gac cag gtt gtg atg acc tcg ctg gcc ttg gtt ggg gct gcc 4537
Trp Asp Gln Val Val Met Thr Ser Leu Ala Leu Val Gly Ala Ala
1460 1465 1470
ctc cat cca ttt gct ctt ctg ctg gtc ctt gct ggg tgg ctg ttt 4582
Leu His Pro Phe Ala Leu Leu Leu Val Leu Ala Gly Trp Leu Phe
1475 1480 1485
cat gtc agg gga gct agg aga agt ggg gat gtc ttg tgg gat att 4627
His Val Arg Gly Ala Arg Arg Ser Gly Asp Val Leu Trp Asp Ile
1490 1495 1500
ccc act cct aag atc atc gag gaa tgt gaa cat ctg gag gat ggg 4672
Pro Thr Pro Lys Ile Ile Glu Glu Cys Glu His Leu Glu Asp Gly
1505 1510 1515
att tat ggc ata ttc cag tca acc ttc ttg ggg gcc tcc cag cga 4717
Ile Tyr Gly Ile Phe Gln Ser Thr Phe Leu Gly Ala Ser Gln Arg
1520 1525 1530
gga gtg gga gtg gca cag gga ggg gtg ttc cac aca atg tgg cat 4762
Gly Val Gly Val Ala Gln Gly Gly Val Phe His Thr Met Trp His
1535 1540 1545
gtc aca aga gga gct ttc ctt gtc agg aat ggc aag aag ttg att 4807
Val Thr Arg Gly Ala Phe Leu Val Arg Asn Gly Lys Lys Leu Ile
1550 1555 1560
cca tct tgg gct tca gta aag gaa gac ctt gtc gcc tat ggt ggc 4852
Pro Ser Trp Ala Ser Val Lys Glu Asp Leu Val Ala Tyr Gly Gly
1565 1570 1575
tca tgg aag ttg gaa ggc aga tgg gat gga gag gaa gag gtc cag 4897
Ser Trp Lys Leu Glu Gly Arg Trp Asp Gly Glu Glu Glu Val Gln
1580 1585 1590
ttg atc gcg gct gtt cca gga aag aac gtg gtc aac gtc cag aca 4942
Leu Ile Ala Ala Val Pro Gly Lys Asn Val Val Asn Val Gln Thr
1595 1600 1605
aaa ccg agc ttg ttc aaa gtg agg aat ggg gga gaa atc ggg gct 4987
Lys Pro Ser Leu Phe Lys Val Arg Asn Gly Gly Glu Ile Gly Ala
1610 1615 1620
gtc gct ctt gac tat ccg agt ggc act tca gga tct cct att gtt 5032
Val Ala Leu Asp Tyr Pro Ser Gly Thr Ser Gly Ser Pro Ile Val
1625 1630 1635
aac agg aac gga gag gtg att ggg ctg tac ggc aat ggc atc ctt 5077
Asn Arg Asn Gly Glu Val Ile Gly Leu Tyr Gly Asn Gly Ile Leu
1640 1645 1650
gtc ggt gac aac tcc ttc gtg tcc gcc ata tcc cag act gag gtg 5122
Val Gly Asp Asn Ser Phe Val Ser Ala Ile Ser Gln Thr Glu Val
1655 1660 1665
aag gaa gaa gga aag gag gag ctc caa gag atc ccg aca atg cta 5167
Lys Glu Glu Gly Lys Glu Glu Leu Gln Glu Ile Pro Thr Met Leu
1670 1675 1680
aag aaa gga atg aca act gtc ctt gat ttt cat cct gga gct ggg 5212
Lys Lys Gly Met Thr Thr Val Leu Asp Phe His Pro Gly Ala Gly
1685 1690 1695
aag aca aga cgt ttc ctc cca cag atc ttg gcc gag tgc gca cgg 5257
Lys Thr Arg Arg Phe Leu Pro Gln Ile Leu Ala Glu Cys Ala Arg
1700 1705 1710
aga cgc ttg cgc act ctt gtg ttg gcc ccc acc agg gtt gtt ctt 5302
Arg Arg Leu Arg Thr Leu Val Leu Ala Pro Thr Arg Val Val Leu
1715 1720 1725
tct gaa atg aag gag gct ttt cac ggc ctg gac gtg aaa ttc cac 5347
Ser Glu Met Lys Glu Ala Phe His Gly Leu Asp Val Lys Phe His
1730 1735 1740
aca cag gct ttt tcc gct cac ggc agc ggg aga gaa gtc att gat 5392
Thr Gln Ala Phe Ser Ala His Gly Ser Gly Arg Glu Val Ile Asp
1745 1750 1755
gcc atg tgc cat gcc acc cta act tac agg atg ttg gaa cca act 5437
Ala Met Cys His Ala Thr Leu Thr Tyr Arg Met Leu Glu Pro Thr
1760 1765 1770
agg gtt gtt aac tgg gaa gtg atc att atg gat gaa gcc cat ttt 5482
Arg Val Val Asn Trp Glu Val Ile Ile Met Asp Glu Ala His Phe
1775 1780 1785
ttg gat cca gcc agc ata gcc gct aga ggt tgg gca gcg cac aga 5527
Leu Asp Pro Ala Ser Ile Ala Ala Arg Gly Trp Ala Ala His Arg
1790 1795 1800
gct agg gca aat gaa agt gca aca atc ttg atg aca gcc aca ccg 5572
Ala Arg Ala Asn Glu Ser Ala Thr Ile Leu Met Thr Ala Thr Pro
1805 1810 1815
cct ggg act agt gat gaa ttt cca cat tca aat ggt gaa ata gaa 5617
Pro Gly Thr Ser Asp Glu Phe Pro His Ser Asn Gly Glu Ile Glu
1820 1825 1830
gat gtt caa acg gac ata ccc agt gag ccc tgg aac aca ggg cat 5662
Asp Val Gln Thr Asp Ile Pro Ser Glu Pro Trp Asn Thr Gly His
1835 1840 1845
gac tgg atc ctg gct gac aaa agg ccc acg gca tgg ttc ctt cca 5707
Asp Trp Ile Leu Ala Asp Lys Arg Pro Thr Ala Trp Phe Leu Pro
1850 1855 1860
tcc atc aga gct gca aat gtc atg gct gcc tct ttg cgt aag gct 5752
Ser Ile Arg Ala Ala Asn Val Met Ala Ala Ser Leu Arg Lys Ala
1865 1870 1875
gga aag agt gtg gtg gtc ctg aac agg aaa acc ttt gag aga gaa 5797
Gly Lys Ser Val Val Val Leu Asn Arg Lys Thr Phe Glu Arg Glu
1880 1885 1890
tac ccc acg ata aag cag aag aaa cct gac ttt ata ttg gcc act 5842
Tyr Pro Thr Ile Lys Gln Lys Lys Pro Asp Phe Ile Leu Ala Thr
1895 1900 1905
gac ata gct gaa atg gga gcc aac ctt tgc gtg gag cga gtg ctg 5887
Asp Ile Ala Glu Met Gly Ala Asn Leu Cys Val Glu Arg Val Leu
1910 1915 1920
gat tgc agg acg gct ttt aag cct gtg ctt gtg gat gaa ggg agg 5932
Asp Cys Arg Thr Ala Phe Lys Pro Val Leu Val Asp Glu Gly Arg
1925 1930 1935
aag gtg gca ata aaa ggg cca ctt cgt atc tcc gca tcc tct gct 5977
Lys Val Ala Ile Lys Gly Pro Leu Arg Ile Ser Ala Ser Ser Ala
1940 1945 1950
gct caa agg agg ggg cgc att ggg aga aat ccc aac aga gat gga 6022
Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro Asn Arg Asp Gly
1955 1960 1965
gac tca tac tac tat tct gag cct aca agt gaa aat aat gcc cac 6067
Asp Ser Tyr Tyr Tyr Ser Glu Pro Thr Ser Glu Asn Asn Ala His
1970 1975 1980
cac gtc tgc tgg ttg gag gcc tca atg ctc ttg gac aac atg gag 6112
His Val Cys Trp Leu Glu Ala Ser Met Leu Leu Asp Asn Met Glu
1985 1990 1995
gtg agg ggt gga atg gtc gcc cca ctc tat ggc gtt gaa gga act 6157
Val Arg Gly Gly Met Val Ala Pro Leu Tyr Gly Val Glu Gly Thr
2000 2005 2010
aaa aca cca gtt tcc cct ggt gaa atg aga ctg agg gat gac cag 6202
Lys Thr Pro Val Ser Pro Gly Glu Met Arg Leu Arg Asp Asp Gln
2015 2020 2025
agg aaa gtc ttc aga gaa cta gtg agg aat tgt gac ctg ccc gtt 6247
Arg Lys Val Phe Arg Glu Leu Val Arg Asn Cys Asp Leu Pro Val
2030 2035 2040
tgg ctt tcg tgg caa gtg gcc aag gct ggt ttg aag acg aat gat 6292
Trp Leu Ser Trp Gln Val Ala Lys Ala Gly Leu Lys Thr Asn Asp
2045 2050 2055
cgt aag tgg tgt ttt gaa ggc cct gag gaa cat gag atc ttg aat 6337
Arg Lys Trp Cys Phe Glu Gly Pro Glu Glu His Glu Ile Leu Asn
2060 2065 2070
gac agc ggt gaa aca gtg aag tgc agg gct cct gga gga gca aag 6382
Asp Ser Gly Glu Thr Val Lys Cys Arg Ala Pro Gly Gly Ala Lys
2075 2080 2085
aag cct ctg cgc cca agg tgg tgt gat gaa agg gtg tca tct gac 6427
Lys Pro Leu Arg Pro Arg Trp Cys Asp Glu Arg Val Ser Ser Asp
2090 2095 2100
cag agt gcg ctg tct gaa ttt att aag ttt gct gaa ggt agg agg 6472
Gln Ser Ala Leu Ser Glu Phe Ile Lys Phe Ala Glu Gly Arg Arg
2105 2110 2115
gga gct gct gaa gtg cta gtt gtg ctg agt gaa ctc cct gat ttc 6517
Gly Ala Ala Glu Val Leu Val Val Leu Ser Glu Leu Pro Asp Phe
2120 2125 2130
ctg gct aaa aaa ggt gga gag gca atg gat acc atc agt gtg ttc 6562
Leu Ala Lys Lys Gly Gly Glu Ala Met Asp Thr Ile Ser Val Phe
2135 2140 2145
ctc cac tct gag gaa ggc tct agg gct tac cgc aat gca cta tca 6607
Leu His Ser Glu Glu Gly Ser Arg Ala Tyr Arg Asn Ala Leu Ser
2150 2155 2160
atg atg cct gag gca atg aca ata gtc atg ctg ttt ata ctg gct 6652
Met Met Pro Glu Ala Met Thr Ile Val Met Leu Phe Ile Leu Ala
2165 2170 2175
gga cta ctg aca tcg gga atg gtc atc ttt ttc atg tct ccc aaa 6697
Gly Leu Leu Thr Ser Gly Met Val Ile Phe Phe Met Ser Pro Lys
2180 2185 2190
ggc atc agt aga atg tct atg gcg atg ggc aca atg gcc ggc tgt 6742
Gly Ile Ser Arg Met Ser Met Ala Met Gly Thr Met Ala Gly Cys
2195 2200 2205
gga tat ctc atg ttc ctt gga ggc gtc aaa ccc act cac atc tcc 6787
Gly Tyr Leu Met Phe Leu Gly Gly Val Lys Pro Thr His Ile Ser
2210 2215 2220
tat gtc atg ctc ata ttc ttt gtc ctg atg gtg gtt gtg atc ccc 6832
Tyr Val Met Leu Ile Phe Phe Val Leu Met Val Val Val Ile Pro
2225 2230 2235
gag cca ggg caa caa agg tcc atc caa gac aac caa gtg gca tac 6877
Glu Pro Gly Gln Gln Arg Ser Ile Gln Asp Asn Gln Val Ala Tyr
2240 2245 2250
ctc att att ggc atc ctg acg ctg gtt tca gcg gtg gca gcc aac 6922
Leu Ile Ile Gly Ile Leu Thr Leu Val Ser Ala Val Ala Ala Asn
2255 2260 2265
gag cta ggc atg ctg gag aaa acc aaa gag gac ctc ttt ggg aag 6967
Glu Leu Gly Met Leu Glu Lys Thr Lys Glu Asp Leu Phe Gly Lys
2270 2275 2280
aag aac tta att cca tct agt gct tca ccc tgg agt tgg ccg gat 7012
Lys Asn Leu Ile Pro Ser Ser Ala Ser Pro Trp Ser Trp Pro Asp
2285 2290 2295
ctt gac ctg aag cca gga gct gcc tgg aca gtg tac gtt ggc att 7057
Leu Asp Leu Lys Pro Gly Ala Ala Trp Thr Val Tyr Val Gly Ile
2300 2305 2310
gtt aca atg ctc tct cca atg ttg cac cac tgg atc aaa gtc gaa 7102
Val Thr Met Leu Ser Pro Met Leu His His Trp Ile Lys Val Glu
2315 2320 2325
tat ggc aac ctg tct ctg tct gga ata gcc cag tca gcc tca gtc 7147
Tyr Gly Asn Leu Ser Leu Ser Gly Ile Ala Gln Ser Ala Ser Val
2330 2335 2340
ctt tct ttc atg gac aag ggg ata cca ttc atg aag atg aat atc 7192
Leu Ser Phe Met Asp Lys Gly Ile Pro Phe Met Lys Met Asn Ile
2345 2350 2355
tcg gtc ata atg ctg ctg gtc agt ggc tgg aat tca ata aca gtg 7237
Ser Val Ile Met Leu Leu Val Ser Gly Trp Asn Ser Ile Thr Val
2360 2365 2370
atg cct ctg ctc tgt ggc ata ggg tgc gcc atg ctc cac tgg tct 7282
Met Pro Leu Leu Cys Gly Ile Gly Cys Ala Met Leu His Trp Ser
2375 2380 2385
ctc att tta cct gga atc aaa gcg cag cag tca aag ctt gca cag 7327
Leu Ile Leu Pro Gly Ile Lys Ala Gln Gln Ser Lys Leu Ala Gln
2390 2395 2400
aga agg gtg ttc cat ggc gtt gcc aag aac cct gtg gtt gat ggg 7372
Arg Arg Val Phe His Gly Val Ala Lys Asn Pro Val Val Asp Gly
2405 2410 2415
aat cca aca gtt gac att gag gaa gct cct gaa atg cct gcc ctt 7417
Asn Pro Thr Val Asp Ile Glu Glu Ala Pro Glu Met Pro Ala Leu
2420 2425 2430
tat gag aag aaa ctg gct cta tat ctc ctt ctt gct ctc agc cta 7462
Tyr Glu Lys Lys Leu Ala Leu Tyr Leu Leu Leu Ala Leu Ser Leu
2435 2440 2445
gct tct gtt gcc atg tgc aga acg ccc ttt tca ttg gct gaa ggc 7507
Ala Ser Val Ala Met Cys Arg Thr Pro Phe Ser Leu Ala Glu Gly
2450 2455 2460
att gtc cta gca tca gct gcc tta ggg ccg ctc ata gag gga aae 7552
Ile Val Leu Ala Ser Ala Ala Leu Gly Pro Leu Ile Glu Gly Asn
2465 2470 2475
acc agc ctt ctt tgg aat gga ccc atg gct gtc tcc atg aca gga 7597
Thr Ser Leu Leu Trp Asn Gly Pro Met Ala Val Ser Met Thr Gly
2480 2485 2490
gtc atg agg ggg aat cac tat gct ttt gtg gga gtc atg tac aat 7642
Val Met Arg Gly Asn His Tyr Ala Phe Val Gly Val Met Tyr Asn
2495 2500 2505
cta tgg aag atg aaa act gga cgc cgg ggg agc gcg aat gga aaa 7687
Leu Trp Lys Met Lys Thr Gly Arg Arg Gly Ser Ala Asn Gly Lys
2510 2515 2520
act ttg ggt gaa gtc tgg aag agg gaa ctg aat ctg ttg gac aag 7732
Thr Leu Gly Glu Val Trp Lys Arg Glu Leu Asn Leu Leu Asn Lys
2525 2530 2535
cga cag ttt gag ttg tat aaa agg acc gac att gtg gag gtg gat 7777
Arg Gln Phe Glu Leu Tyr Lys Arg Thr Asp Ile Val Glu Val Asp
2540 2545 2550
cgt gat acg gca cgc agg cat ttg gcc gaa ggg aag gtg gac acc 7822
Arg Asp Thr Ala Arg Arg His Leu Ala Glu Gly Lys Val Asp Thr
2555 2560 2565
ggg gtg gcg gtc tcc agg ggg acc gca aag tta agg tgg ttc cat 7867
Gly Val Ala Val Ser Arg Gly Thr Ala Lys Leu Arg Trp Phe His
2570 2575 2580
gag cgt ggc tat gtc aag ctg gaa ggt agg gtg att gac ctg ggg 7912
Glu Arg Gly Tyr Val Lys Leu Glu Gly Arg Val Ile Asp Leu Gly
2585 2590 2595
tgt ggc cgc gga ggc tgg tgt tac tac gct gct gcg caa aag gaa 7957
Cys Gly Arg Gly Gly Trp Cys Tyr Tyr Ala Ala Ala Gln Lys Glu
2600 2605 2610
gtg agt ggg gtc aaa gga ttt act ctt gga aga gac ggc cat gag 8002
Val Ser Gly Val Lys Gly Phe Thr Leu Gly Arg Asp Gly His Glu
2615 2620 2625
aaa ccc atg aat gtg caa agt ctg gga tgg aac atc atc acc ttc 8047
Lys Pro Met Asn Val Gln Ser Leu Gly Trp Asn Ile Ile Thr Phe
2630 2635 2640
aag gac aaa act gat atc cac cgc cta gaa cca gtg aaa tgt gac 8092
Lys Asp Lys Thr Asp Ile His Arg Leu Glu Pro Val Lys Cys Asp
2645 2650 2655
acc ctt ttg tgt gac att gga gag tca tca tcg tca tcg gtc aca 8137
Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Ser Ser Val Thr
2660 2665 2670
gag ggg gaa agg acc gtg aga gtt ctt gat act gta gaa aaa tgg 8182
Glu Gly Glu Arg Thr Val Arg Val Leu Asp Thr Val Glu Lys Trp
2675 2680 2685
ctg gct tgt ggg gtt gac aac ttc tgt gtg aag gtg tta gct cca 8227
Leu Ala Cys Gly Val Asp Asn Phe Cys Val Lys Val Leu Ala Pro
2690 2695 2700
tac atg cca gat gtt ctt gag aaa ctg gaa ttg ctc caa agg agg 8272
Tyr Met Pro Asp Val Leu Glu Lys Leu Glu Leu Leu Gln Arg Arg
2705 2710 2715
ttt ggc gga aca gtg atc agg aac cct ctc tcc agg aat tcc act 8317
Phe Gly Gly Thr Val Ile Arg Asn Pro Leu Ser Arg Asn Ser Thr
2720 2725 2730
cat gaa atg tac tac gtg tct gga gcc cgc agc aat gtc aca ttt 8362
His Glu Met Tyr Tyr Val Ser Gly Ala Arg Ser Asn Val Thr Phe
2735 2740 2745
act gtg aac caa aca tcc cgc ctc ctg atg agg aga atg agg cgt 8407
Thr Val Asn Gln Thr Ser Arg Leu Leu Met Arg Arg Met Arg Arg
2750 2755 2760
cca act gga aaa gtg acc ctg gag gct gac gtc atc ctc cca att 8452
Pro Thr Gly Lys Val Thr Leu Glu Ala Asp Val Ile Leu Pro Ile
2765 2770 2775
ggg aca cgc agt gtt gag aca gac aag gga ccc ctg gac aaa gag 8497
Gly Thr Arg Ser Val Glu Thr Asp Lys Gly Pro Leu Asp Lys Glu
2780 2785 2790
gcc ata gaa gaa agg gtt gag agg ata aaa tct gag tac atg acc 8542
Ala Ile Glu Glu Arg Val Glu Arg Ile Lys Ser Glu Tyr Met Thr
2795 2800 2805
tct tgg ttt tat gac aat gac aac ccc tac agg acc tgg cac tac 8587
Ser Trp Phe Tyr Asp Asn Asp Asn Pro Tyr Arg Thr Trp His Tyr
2810 2815 2820
tgt ggc tcc tat gtc aca aaa acc tcc gga agt gcg gcg agc atg 8632
Cys Gly Ser Tyr Val Thr Lys Thr Ser Gly Ser Ala Ala Ser Met
2825 2830 2835
gta aat ggt gtt att aaa att ctg aca tat cca tgg gac agg ata 8677
Val Asn Gly Val Ile Lys Ile Leu Thr Tyr Pro Trp Asp Arg Ile
2840 2845 2850
gag gag gtc aca aga atg gca atg act gac aca acc cct ttt gga 8722
Glu Glu Val Thr Arg Met Ala Met Thr Asp Thr Thr Pro Phe Gly
2855 2860 2865
cag caa aga gtg ttt aaa gaa aaa gtt gac acc aga gca aag gat 8767
Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Ala Lys Asp
2870 2875 2880
cca cca gcg gga act agg aag atc atg aaa gtt gtc aac agg tgg 8812
Pro Pro Ala Gly Thr Arg Lys Ile Met Lys Val Val Asn Arg Trp
2885 2890 2895
ctg ttc cgc cac ctg gcc aga gaa aag aac ccc aga ctg tgc aca 8857
Leu Phe Arg His Leu Ala Arg Glu Lys Asn Pro Arg Leu Cys Thr
2900 2905 2910
aag gaa gaa ttt att gca aaa gtc cga agt cat gca gcc att gga 8902
Lys Glu Glu Phe Ile Ala Lys Val Arg Ser His Ala Ala Ile Gly
2915 2920 2925
gct tac ctg gaa gaa caa gaa cag tgg aag act gcc aat gag gct 8947
Ala Tyr Leu Glu Glu Gln Glu Gln Trp Lys Thr Ala Asn Glu Ala
2930 2935 2940
gtc caa gac cca aag ttc tgg gaa ctg gtg gat gaa gaa agg aag 8992
Val Gln Asp Pro Lys Phe Trp Glu Leu Val Asp Glu Glu Arg Lys
2945 2950 2955
ctg cac caa caa ggc agg tgt cgg act tgt gtg tac aac atg atg 9037
Leu His Gln Gln Gly Arg Cys Arg Thr Cys Val Tyr Asn Met Met
2960 2965 2970
ggg aaa aga gag aag aag ctg tca gag ttt ggg aaa gca aag gga 9082
Gly Lys Arg Glu Lys Lys Leu Ser Glu Phe Gly Lys Ala Lys Gly
2975 2980 2985
agc cgt gcc ata tgg tat atg tgg ctg gga gcg cgg tat ctt gag 9127
Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Tyr Leu Glu
2990 2995 3000
ttt gag gcc ctg gga ttc ctg aat gag gac cat tgg gct tcc agg 9172
Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Ala Ser Arg
3005 3010 3015
gaa aac tca gga gga gga gtg gaa ggc att ggc tta caa tac cta 9217
Glu Asn Ser Gly Gly Gly Val Glu Gly Ile Gly Leu Gln Tyr Leu
3020 3025 3030
gga tat gtg atc aga gac ctg gct gca atg gat ggt ggt gga ttc 9262
Gly Tyr Val Ile Arg Asp Leu Ala Ala Met Asp Gly Gly Gly Phe
3035 3040 3045
tac gcg gat gac acc gct gga tgg gac acg cgc atc aca gag gca 9307
Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu Ala
3050 3055 3060
gac ctt gat gat gaa cag gag atc ttg aac tac atg agc cca cat 9352
Asp Leu Asp Asp Glu Gln Glu Ile Leu Asn Tyr Met Ser Pro His
3065 3070 3075
cac aaa aaa ctg gca caa gca gtg atg gaa atg aca tac aag aac 9397
His Lys Lys Leu Ala Gln Ala Val Met Glu Met Thr Tyr Lys Asn
3080 3085 3090
aaa gtg gtg aaa gtg ttg aga cca gcc cca gga ggg aaa gcc tac 9442
Lys Val Val Lys Val Leu Arg Pro Ala Pro Gly Gly Lys Ala Tyr
3095 3100 3105
atg gat gtc ata agt cga cga gac cag aga gga tcc ggg cag gta 9487
Met Asp Val Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly Gln Val
3110 3115 3120
gtg act tat gct ctg aac acc atc acc aac ttg aaa gtc caa ttg 9532
Val Thr Tyr Ala Leu Asn Thr Ile Thr Asn Leu Lys Val Gln Leu
3125 3130 3135
atc aga atg gca gaa gca gag atg gtg ata cat cac caa cat gtt 9577
Ile Arg Met Ala Glu Ala Glu Met Val Ile His His Gln His Val
3140 3145 3150
caa gat tgt gat gaa tca gtt ctg acc agg ctg gag gca tgg ctc 9622
Gln Asp Cys Asp Glu Ser Val Leu Thr Arg Leu Glu Ala Trp Leu
3155 3160 3165
act gag cac gga tgt gac aga ctg aag agg atg gcg gtg agt gga 9667
Thr Glu His Gly Cys Asp Arg Leu Lys Arg Met Ala Val Ser Gly
3170 3175 3180
gac gac tgt gtg gtc cgg ccc atc gat gac agg ttc ggc ctg gcc 9712
Asp Asp Cys Val Val Arg Pro Ile Asp Asp Arg Phe Gly Leu Ala
3185 3190 3195
ctg tcc cat ctc aac gcc atg tcc aag gtt aga aag gac ata tct 9757
Leu Ser His Leu Asn Ala Met Ser Lys Val Arg Lys Asp Ile Ser
3200 3205 3210
gaa tgg cag cca tca aaa ggg tgg aat gat tgg gag aat gtg ccc 9802
Glu Trp Gln Pro Ser Lys Gly Trp Asn Asp Trp Glu Asn Val Pro
3215 3220 3225
ttc tgt tcc cac cac ttc cat gaa cta cag ctg aag gat ggc agg 9847
Phe Cys Ser His His Phe His Glu Leu Gln Leu Lys Asp Gly Arg
3230 3235 3240
agg att gtg gtg cct tgc cga gaa cag gac gag ctc att ggg aga 9892
Arg Ile Val Val Pro Cys Arg Glu Gln Asp Glu Leu Ile Gly Arg
3245 3250 3255
gga agg gtg tct cca gga aac ggc tgg atg atc aag gaa aca gct 9937
Gly Arg Val Ser Pro Gly Asn Gly Trp Met Ile Lys Glu Thr Ala
3260 3265 3270
tgc ctc agc aaa gcc tat gcc aac atg tgg tca ctg atg tat ttt 9982
Cys Leu Ser Lys Ala Tyr Ala Asn Met Trp Ser Leu Met Tyr Phe
3275 3280 3285
cac aaa agg gac atg agg cta ctg tca ttg gct gtt tcc tca gct 10027
His Lys Arg Asp Met Arg Leu Leu Ser Leu Ala Val Ser Ser Ala
3290 3295 3300
gtt ccc acc tca tgg gtt cca caa gga cgc aca aca tgg tcg att 10072
Val Pro Thr Ser Trp Val Pro Gln Gly Arg Thr Thr Trp Ser Ile
3305 3310 3315
cat ggg aaa ggg gag tgg atg acc acg gaa gac atg ctt gag gtg 10117
His Gly Lys Gly Glu Trp Met Thr Thr Glu Asp Met Leu Glu Val
3320 3325 3330
tgg aac aga gta tgg ata acc aac aac cca cac atg cag gac aag 10162
Trp Asn Arg Val Trp Ile Thr Asn Asn Pro His Met Gln Asp Lys
3335 3340 3345
aca atg gtg aaa aaa tgg aga gat gtc cct tat cta acc aag aga 10207
Thr Met Val Lys Lys Trp Arg Asp Val Pro Tyr Leu Thr Lys Arg
3350 3355 3360
caa gac aag ctg tgc gga tca ctg att gga atg acc aat agg gcc 10252
Gln Asp Lys Leu Cys Gly Ser Leu Ile Gly Met Thr Asn Arg Ala
3365 3370 3375
acc tgg gcc tcc cac atc cat tta gtc atc cat cgt atc cga acg 10297
Thr Trp Ala Ser His Ile His Leu Val Ile His Arg Ile Arg Thr
3380 3385 3390
ctg att gga cag gag aaa tac act gac tac cta aca gtc atg gac 10342
Leu Ile Gly Gln Glu Lys Tyr Thr Asp Tyr Leu Thr Val Met Asp
3395 3400 3405
agg tat tct gtg gat gct gac ctg caa ctg ggt gag ctt atc 10384
Arg Tyr Ser Val Asp Ala Asp Leu Gln Leu Gly Glu Leu Ile
3410 3415 3420
tgaaacacca tctaacagga ataaccggga tacaaaccac gggtggagaa ccggactccc 10444
cacaacctga aaccgggata taaaccacgg ctggagaacc ggactccgca cttaaaatga 10504
aacagaaacc gggataaaaa ctacggatgg agaaccggac tccacacatt gagacagaag 10564
aagttgtcag cccagaaccc cacacgagtt ttgccactgc taagctgtga ggcagtgcag 10624
gctgggacag ccgacctcca ggttgcgaaa aacctggttt ctgggacctc ccaccccaga 10684
gtaaaaagaa cggagcctcc gctaccaccc tcccacgtgg tggtagaaag acggggtcta 10744
gaggttagag gagaccctcc agggaacaaa tagtgggacc atattgacgc cagggaaaga 10804
ccggagtggt tctctgcttt tcctccagag gtctgtgagc acagtttgct caagaataag 10864
cagacctttg gatgacaaac acaaaaccac aa 10896
<210>23
<211>3422
<212>PRT
<213>人工的
<220>
<223>合成的构建体
<400>23
Met Ser Gly Arg Lys Ala Gln Gly Lys Thr Leu Gly Val Asn Met Val
1 5 10 15
Arg Arg Gly Val Arg Ser Leu Ser Asn Lys Ile Lys Gln Lys Thr Lys
20 25 30
Gln Ile Gly Asn Arg Pro Gly Pro Ser Arg Gly Val Gln Gly Phe Ile
35 40 45
Phe Phe Phe Leu Phe Asn Ile Leu Thr Gly Lys Lys Ile Thr Ala His
50 55 60
Leu Lys Arg Leu Trp Lys Met Leu Asp Pro Arg Gln Gly Leu Ala Val
65 70 75 80
Leu Arg Lys Val Lys Arg Val Val Ala Ser Leu Met Arg Gly Leu Ser
85 90 95
Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val Gln Phe Leu Ile
100 105 110
Leu Gly Met Leu Leu Met Thr Gly Gly Val Thr Leu Ser Asn Phe Gln
115 120 125
Gly Lys Val Met Met Thr Val Asn Ala Thr Asp Val Thr Asp Val Ile
130 135 140
Thr Ile Pro Thr Ala Ala Gly Lys Asn Leu Cys Ile Val Arg Ala Met
145 150 155 160
Asp Val Gly Tyr Met Cys Asp Asp Thr Ile Thr Tyr Glu Cys Pro Val
165 170 175
Leu Ser Ala Gly Asn Asp Pro Glu Asp Ile Asp Cys Trp Cys Thr Lys
180 185 190
Ser Ala Val Tyr Val Arg Tyr Gly Arg Cys Thr Lys Thr Arg His Ser
195 200 205
Arg Arg Ser Arg Arg Ser Leu Thr Val Gln Thr His Gly Glu Ser Thr
210 215 220
Leu Ala Asn Lys Lys Gly Ala Trp Met Asp Ser Thr Lys Ala Thr Arg
225 230 235 240
Tyr Leu Val Lys Thr Glu Ser Trp Ile Leu Arg Asn Pro Gly Tyr Ala
245 250 255
Leu Val Ala Ala Val Ile Gly Trp Met Leu Gly Ser Asn Thr Met Gln
260 265 270
Arg Val Val Phe Val Val Pro Leu Leu Leu Val Ala Pro Ala Tyr Ser
275 280 285
Phe Asn Cys Leu Gly Met Ser Asn Arg Asp Phe Leu Glu Gly Val Ser
290 295 300
Gly Ala Thr Trp Val Asp Leu Val Leu Glu Gly Asp Ser Cys Val Thr
305 310 315 320
Ile Met Ser Lys Asp Lys Pro Thr Ile Asp Val Lys Met Met Asn Met
325 330 335
Glu Ala Ala Asn Leu Ala Glu Val Arg Ser Tyr Cys Tyr Leu Ala Thr
340 345 350
Val Ser Asp Leu Ser Thr Lys Ala Ala Cys Pro Thr Met Gly Glu Ala
355 360 365
His Asn Asp Lys Arg Ala Asp Pro Ala Phe Val Cys Arg Gln Gly Val
370 375 380
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Phe Phe Gly Lys Gly Ser
385 390 395 400
Ile Asp Thr Cys Ala Lys Phe Ala Cys Ser Thr Lys Ala Ile Gly Arg
405 410 415
Thr Ile Leu Lys Glu Asn Ile Lys Tyr Glu Val Ala Ile Phe Val His
420 425 430
Gly Pro Thr Thr Val Glu Ser His Gly Asn Tyr Ser Thr Gln Val Gly
435 440 445
Ala Thr Gln Ala Gly Arg Phe Ser Ile Thr Pro Ala Ala Pro Ser Tyr
450 455 460
Thr Leu Lys Leu Gly Glu Tyr Gly Glu Val Thr Val Asp Cys Glu Pro
465 470 475 480
Arg Ser Gly Ile Asp Thr Asn Ala Tyr Tyr Val Met Thr Val Gly Thr
485 490 495
Lys Thr Phe Leu Val His Arg Glu Trp Phe Met Asp Leu Asn Leu Pro
500 505 510
Trp Ser Ser Ala Gly Ser Thr Val Trp Arg Asn Arg Glu Thr Leu Met
515 520 525
Glu Phe Glu Glu Pro His Ala Thr Lys Gln Ser Val Ile Ala Leu Gly
530 535 540
Ser Gln Glu Gly Ala Leu His Gln Ala Leu Ala Gly Ala Ile Pro Val
545 550 555 560
Glu Phe Ser Ser Asn Thr Val Lys Leu Thr Ser Gly His Leu Lys Cys
565 570 575
Arg Val Lys Met Glu Lys Leu Gln Leu Lys Gly Thr Thr Tyr Gly Val
580 585 590
Cys Ser Lys Ala Phe Lys Phe Leu Arg Thr Pro Val Asp Thr Gly His
595 600 605
Gly Thr Val Val Leu Glu Leu Gln Tyr Thr Gly Thr Asp Gly Pro Cys
610 615 620
Lys Val Pro Ile Ser Ser Val Ala Ser Leu Asn Asp Leu Thr Pro Val
625 630 635 640
Gly Arg Leu Val Thr Val Asn Pro Phe Val Ser Val Ala Thr Ala Asn
645 650 655
Ala Lys Val Leu Ile Glu Leu Glu Pro Pro Phe Gly Asp Ser Tyr Ile
660 665 670
Val Val Gly Arg Gly Glu Gln Gln Ile Asn His His Trp His Lys Ser
675 680 685
Gly Ser Ser Ile Gly Lys Ala Phe Thr Thr Thr Leu Lys Gly Ala Gln
690 695 700
Arg Leu Ala Ala Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly
705 710 715 720
Gly Val Phe Thr Ser Val Gly Arg Ala Val His Gln Val Phe Gly Gly
725 730 735
Ala Phe Arg Ser Leu Phe Gly Gly Met Ser Trp Ile Thr Gln Gly Leu
740 745 750
Leu Gly Ala Leu Leu Leu Trp Met Gly Ile Asn Ala Arg Asp Arg Ser
755 760 765
Ile Ala Leu Thr Phe Leu Ala Val Gly Gly Val Leu Leu Phe Leu Ser
770 775 780
Val Asn Val Gly Ala Asp Gln Gly Cys Ala Ile Asn Phe Gly Lys Arg
785 790 795 800
Glu Leu Lys Cys Gly Asp Gly Ile Phe Ile Phe Arg Asp Ser Asp Asp
805 810 815
Trp Leu Asn Lys Tyr Ser Tyr Tyr Pro Glu Asp Pro Val Lys Leu Ala
820 825 830
Ser Ile Val Lys Ala Ser Phe Glu Glu Gly Lys Cys Gly Leu Asn Ser
835 840 845
Val Asp Ser Leu Glu His Glu Met Trp Arg Ser Arg Ala Asp Glu Ile
850 855 860
Asn Ala Ile Phe Glu Glu Asn Glu Val Asp Ile Ser Val Val Val Gln
865 870 875 880
Asp Pro Lys Asn Val Tyr Gln Arg Gly Thr His Pro Phe Ser Arg Ile
885 890 895
Arg Asp Gly Leu Gln Tyr Gly Trp Lys Thr Trp Gly Lys Asn Leu Val
900 905 910
Phe Ser Pro Gly Arg Lys Asn Gly Ser Phe Ile Ile Asp Gly Lys Ser
915 920 925
Arg Lys Glu Cys Pro Phe Ser Asn Arg Val Trp Asn Ser Phe Gln Ile
930 935 940
Glu Glu Phe Gly Thr Gly Val Phe Thr Thr Arg Val Tyr Met Asp Ala
945 950 955 960
Val Phe Glu Tyr Thr Ile Asp Cys Asp Gly Ser Ile Leu Gly Ala Ala
965 970 975
Val Asn Gly Lys Lys Ser Ala His Gly Ser Pro Thr Phe Trp Met Gly
980 985 990
Ser His Glu Val Asn Gly Thr Trp Met Ile His Thr Leu Glu Ala Leu
995 1000 1005
Asp Tyr Lys Glu Cys Glu Trp Pro Leu Thr His Thr Ile Gly Thr
1010 1015 1020
Ser Val Glu Glu Ser Glu Met Phe Met Pro Arg Ser Ile Gly Gly
1025 1030 1035
Pro Val Ser Ser His Asn His Ile Pro Gly Tyr Lys Val Gln Thr
1040 1045 1050
Asn Gly Pro Trp Met Gln Val Pro Leu Glu Val Lys Arg Glu Ala
1055 1060 1065
Cys Pro Gly Thr Ser Val Ile Ile Asp Gly Asn Cys Asp Gly Arg
1070 1075 1080
Gly Lys Ser Thr Arg Ser Thr Thr Asp Ser Gly Lys Val Ile Pro
1085 1090 1095
Glu Trp Cys Cys Arg Ser Cys Thr Met Pro Pro Val Ser Phe His
1100 1105 1110
Gly Ser Asp Gly Cys Trp Tyr Pro Met Glu Ile Arg Pro Arg Lys
1115 1120 1125
Thr His Glu Ser His Leu Val Arg Ser Trp Val Thr Ala Gly Glu
1130 1135 1140
Ile His Ala Val Pro Phe Gly Leu Val Ser Met Met Ile Ala Met
1145 1150 1155
Glu Val Val Leu Arg Lys Arg Gln Gly Pro Lys Gln Met Leu Val
1160 1165 1170
Gly Gly Val Val Leu Leu Gly Ala Met Leu Val Gly Gln Val Thr
1175 1180 1185
Leu Leu Asp Leu Leu Lys Leu Thr Val Ala Val Gly Leu His Phe
1190 1195 1200
His Glu Met Asn Asn Gly Gly Asp Ala Met Tyr Met Ala Leu Ile
1205 1210 1215
Ala Ala Phe Ser Ile Arg Pro Gly Leu Leu Ile Gly Phe Gly Leu
1220 1225 1230
Arg Thr Leu Trp Ser Pro Arg Glu Arg Leu Val Leu Thr Leu Gly
1235 1240 1245
Ala Ala Met Val Glu Ile Ala Leu Gly Gly Val Met Gly Gly Leu
1250 1255 1260
Trp Lys Tyr Leu Asn Ala Val Ser Leu Cys Ile Leu Thr Ile Asn
1265 1270 1275
Ala Val Ala Ser Arg Lys Ala Ser Asn Thr Ile Leu Pro Leu Met
1280 1285 1290
Ala Leu Leu Thr Pro Val Thr Met Ala Glu Val Arg Leu Ala Ala
1295 1300 1305
Met Phe Phe Cys Ala Met Val Ile Ile Gly Val Leu His Gln Asn
1310 1315 1320
Phe Lys Asp Thr Ser Met Gln Lys Thr Ile Pro Leu Val Ala Leu
1325 1330 1335
Thr Leu Thr Ser Tyr Leu Gly Leu Thr Gln Pro Phe Leu Gly Leu
1340 1345 1350
Cys Ala Phe Leu Ala Thr Arg Ile Phe Gly Arg Arg Ser Ile Pro
1355 1360 1365
Val Asn Glu Ala Leu Ala Ala Ala Gly Leu Val Gly Val Leu Ala
1370 1375 1380
Gly Leu Ala Phe Gln Glu Met Glu Asn Phe Leu Gly Pro Ile Ala
1385 1390 1395
Val Gly Gly Leu Leu Met Met Leu Val Ser Val Ala Gly Arg Val
1400 1405 1410
Asp Gly Leu Glu Leu Lys Lys Leu Gly Glu Val Ser Trp Glu Glu
1415 1420 1425
Glu Ala Glu Ile Ser Gly Ser Ser Ala Arg Tyr Asp Val Ala Leu
1430 1435 1440
Ser Glu Gln Gly Glu Phe Lys Leu Leu Ser Glu Glu Lys Val Pro
1445 1450 1455
Trp Asp Gln Val Val Met Thr Ser Leu Ala Leu Val Gly Ala Ala
1460 1465 1470
Leu His Pro Phe Ala Leu Leu Leu Val Leu Ala Gly Trp Leu Phe
1475 1480 1485
His Val Arg Gly Ala Arg Arg Ser Gly Asp Val Leu Trp Asp Ile
1490 1495 1500
Pro Thr Pro Lys Ile Ile Glu Glu Cys Glu His Leu Glu Asp Gly
1505 1510 1515
Ile Tyr Gly Ile Phe Gln Ser Thr Phe Leu Gly Ala Ser Gln Arg
1520 1525 1530
Gly Val Gly Val Ala Gln Gly Gly Val Phe His Thr Met Trp His
1535 1540 1545
Val Thr Arg Gly Ala Phe Leu Val Arg Asn Gly Lys Lys Leu Ile
1550 1555 1560
Pro Ser Trp Ala Ser Val Lys Glu Asp Leu Val Ala Tyr Gly Gly
1565 1570 1575
Ser Trp Lys Leu Glu Gly Arg Trp Asp Gly Glu Glu Glu Val Gln
1580 1585 1590
Leu Ile Ala Ala Val Pro Gly Lys Asn Val Val Asn Val Gln Thr
1595 1600 1605
Lys Pro Ser Leu Phe Lys Val Arg Asn Gly Gly Glu Ile Gly Ala
1610 1615 1620
Val Ala Leu Asp Tyr Pro Ser Gly Thr Ser Gly Ser Pro Ile Val
1625 1630 1635
Asn Arg Asn Gly Glu Val Ile Gly Leu Tyr Gly Asn Gly Ile Leu
1640 1645 1650
Val Gly Asp Asn Ser Phe Val Ser Ala Ile Ser Gln Thr Glu Val
1655 1660 1665
Lys Glu Glu Gly Lys Glu Glu Leu Gln Glu Ile Pro Thr Met Leu
1670 1675 1680
Lys Lys Gly Met Thr Thr Val Leu Asp Phe His Pro Gly Ala Gly
1685 1690 1695
Lys Thr Arg Arg Phe Leu Pro Gln Ile Leu Ala Glu Cys Ala Arg
1700 1705 1710
Arg Arg Leu Arg Thr Leu Val Leu Ala Pro Thr Arg Val Val Leu
1715 1720 1725
Ser Glu Met Lys Glu Ala Phe His Gly Leu Asp Val Lys Phe His
1730 1735 1740
Thr Gln Ala Phe Ser Ala His Gly Ser Gly Arg Glu Val Ile Asp
1745 1750 1755
Ala Met Cys His Ala Thr Leu Thr Tyr Arg Met Leu Glu Pro Thr
1760 1765 1770
Arg Val Val Asn Trp Glu Val Ile Ile Met Asp Glu Ala His Phe
1775 1780 1785
Leu Asp Pro Ala Ser Ile Ala Ala Arg Gly Trp Ala Ala His Arg
1790 1795 1800
Ala Arg Ala Asn Glu Ser Ala Thr Ile Leu Met Thr Ala Thr Pro
1805 1810 1815
Pro Gly Thr Ser Asp Glu Phe Pro His Ser Asn Gly Glu Ile Glu
1820 1825 1830
Asp Val Gln Thr Asp Ile Pro Ser Glu Pro Trp Asn Thr Gly His
1835 1840 1845
Asp Trp Ile Leu Ala Asp Lys Arg Pro Thr Ala Trp Phe Leu Pro
1850 1855 1860
Ser Ile Arg Ala Ala Asn Val Met Ala Ala Ser Leu Arg Lys Ala
1865 1870 1875
Gly Lys Ser Val Val Val Leu Asn Arg Lys Thr Phe Glu Arg Glu
1880 1885 1890
Tyr Pro Thr Ile Lys Gln Lys Lys Pro Asp Phe Ile Leu Ala Thr
1895 1900 1905
Asp Ile Ala Glu Met Gly Ala Asn Leu Cys Val Glu Arg Val Leu
1910 1915 1920
Asp Cys Arg Thr Ala Phe Lys Pro Val Leu Val Asp Glu Gly Arg
1925 1930 1935
Lys Val Ala Ile Lys Gly Pro Leu Arg Ile Ser Ala Ser Ser Ala
1940 1945 1950
Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro Asn Arg Asp Gly
1955 1960 1965
Asp Ser Tyr Tyr Tyr Ser Glu Pro Thr Ser Glu Asn Asn Ala His
1970 1975 1980
His Val Cys Trp Leu Glu Ala Ser Met Leu Leu Asp Asn Met Glu
1985 1990 1995
Val Arg Gly Gly Met Val Ala Pro Leu Tyr Gly Val Glu Gly Thr
2000 2005 2010
Lys Thr Pro Val Ser Pro Gly Glu Met Arg Leu Arg Asp Asp Gln
2015 2020 2025
Arg Lys Val Phe Arg Glu Leu Val Arg Asn Cys Asp Leu Pro Val
2030 2035 2040
Trp Leu Ser Trp Gln Val Ala Lys Ala Gly Leu Lys Thr Asn Asp
2045 2050 2055
Arg Lys Trp Cys Phe Glu Gly Pro Glu Glu His Glu Ile Leu Asn
2060 2065 2070
Asp Ser Gly Glu Thr Val Lys Cys Arg Ala Pro Gly Gly Ala Lys
2075 2080 2085
Lys Pro Leu Arg Pro Arg Trp Cys Asp Glu Arg Val Ser Ser Asp
2090 2095 2100
Gln Ser Ala Leu Ser Glu Phe Ile Lys Phe Ala Glu Gly Arg Arg
2105 2110 2115
Gly Ala Ala Glu Val Leu Val Val Leu Ser Glu Leu Pro Asp Phe
2120 2125 2130
Leu Ala Lys Lys Gly Gly Glu Ala Met Asp Thr Ile Ser Val Phe
2135 2140 2145
Leu His Ser Glu Glu Gly Ser Arg Ala Tyr Arg Asn Ala Leu Ser
2150 2155 2160
Met Met Pro Glu Ala Met Thr Ile Val Met Leu Phe Ile Leu Ala
2165 2170 2175
Gly Leu Leu Thr Ser Gly Met Val Ile Phe Phe Met Ser Pro Lys
2180 2185 2190
Gly Ile Ser Arg Met Ser Met Ala Met Gly Thr Met Ala Gly Cys
2195 2200 2205
Gly Tyr Leu Met Phe Leu Gly Gly Val Lys Pro Thr His Ile Ser
2210 2215 2220
Tyr Val Met Leu Ile Phe Phe Val Leu Met Val Val Val Ile Pro
2225 2230 2235
Glu Pro Gly Gln Gln Arg Ser Ile Gln Asp Asn Gln Val Ala Tyr
2240 2245 2250
Leu Ile Ile Gly Ile Leu Thr Leu Val Ser Ala Val Ala Ala Asn
2255 2260 2265
Glu Leu Gly Met Leu Glu Lys Thr Lys Glu Asp Leu Phe Gly Lys
2270 2275 2280
Lys Asn Leu Ile Pro Ser Ser Ala Ser Pro Trp Ser Trp Pro Asp
2285 2290 2295
Leu Asp Leu Lys Pro Gly Ala Ala Trp Thr Val Tyr Val Gly Ile
2300 2305 2310
Val Thr Met Leu Ser Pro Met Leu His His Trp Ile Lys Val Glu
2315 2320 2325
Tyr Gly Asn Leu Ser Leu Ser Gly Ile Ala Gln Ser Ala Ser Val
2330 2335 2340
Leu Ser Phe Met Asp Lys Gly Ile Pro Phe Met Lys Met Asn Ile
2345 2350 2355
Ser Val Ile Met Leu Leu Val Ser Gly Trp Asn Ser Ile Thr Val
2360 2365 2370
Met Pro Leu Leu Cys Gly Ile Gly Cys Ala Met Leu His Trp Ser
2375 2380 2385
Leu Ile Leu Pro Gly Ile Lys Ala Gln Gln Ser Lys Leu Ala Gln
2390 2395 2400
Arg Arg Val Phe His Gly Val Ala Lys Asn Pro Val Val Asp Gly
2405 2410 2415
Asn Pro Thr Val Asp Ile Glu Glu Ala Pro Glu Met Pro Ala Leu
2420 2425 2430
Tyr Glu Lys Lys Leu Ala Leu Tyr Leu Leu Leu Ala Leu Ser Leu
2435 2440 2445
Ala Ser Val Ala Met Cys Arg Thr Pro Phe Ser Leu Ala Glu Gly
2450 2455 2460
Ile Val Leu Ala Ser Ala Ala Leu Gly Pro Leu Ile Glu Gly Asn
2465 2470 2475
Thr Ser Leu Leu Trp Asn Gly Pro Met Ala Val Ser Met Thr Gly
2480 2485 2490
Val Met Arg Gly Asn His Tyr Ala Phe Val Gly Val Met Tyr Asn
2495 2500 2505
Leu Trp Lys Met Lys Thr Gly Arg Arg Gly Ser Ala Asn Gly Lys
2510 2515 2520
Thr Leu Gly Glu Val Trp Lys Arg Glu Leu Asn Leu Leu Asp Lys
2525 2530 2535
Arg Gln Phe Glu Leu Tyr Lys Arg Thr Asp Ile Val Glu Val Asp
2540 2545 2550
Arg Asp Thr Ala Arg Arg His Leu Ala Glu Gly Lys Val Asp Thr
2555 2560 2565
Gly Val Ala Val Ser Arg Gly Thr Ala Lys Leu Arg Trp Phe His
2570 2575 2580
Glu Arg Gly Tyr Val Lys Leu Glu Gly Arg Val Ile Asp Leu Gly
2585 2590 2595
Cys Gly Arg Gly Gly Trp Cys Tyr Tyr Ala Ala Ala Gln Lys Glu
2600 2605 2610
Val Ser Gly Val Lys Gly Phe Thr Leu Gly Arg Asp Gly His Glu
2615 2620 2625
Lys Pro Met Asn Val Gln Ser Leu Gly Trp Asn Ile Ile Thr Phe
2630 2635 2640
Lys Asp Lys Thr Asp Ile His Arg Leu Glu Pro Val Lys Cys Asp
2645 2650 2655
Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Ser Ser Val Thr
2660 2665 2670
Glu Gly Glu Arg Thr Val Arg Val Leu Asp Thr Val Glu Lys Trp
2675 2680 2685
Leu Ala Cys Gly Val Asp Asn Phe Cys Val Lys Val Leu Ala Pro
2690 2695 2700
Tyr Met Pro Asp Val Leu Glu Lys Leu Glu Leu Leu Gln Arg Arg
2705 2710 2715
Phe Gly Gly Thr Val Ile Arg Asn Pro Leu Ser Arg Asn Ser Thr
2720 2725 2730
His Glu Met Tyr Tyr Val Ser Gly Ala Arg Ser Asn Val Thr Phe
2735 2740 2745
Thr Val Asn Gln Thr Ser Arg Leu Leu Met Arg Arg Met Arg Arg
2750 2755 2760
Pro Thr Gly Lys Val Thr Leu Glu Ala Asp Val Ile Leu Pro Ile
2765 2770 2775
Gly Thr Arg Ser Val Glu Thr Asp Lys Gly Pro Leu Asp Lys Glu
2780 2785 2790
Ala Ile Glu Glu Arg Val Glu Arg Ile Lys Ser Glu Tyr Met Thr
2795 2800 2805
Ser Trp Phe Tyr Asp Asn Asp Asn Pro Tyr Arg Thr Trp His Tyr
2810 2815 2820
Cys Gly Ser Tyr Val Thr Lys Thr Ser Gly Ser Ala Ala Ser Met
2825 2830 2835
Val Asn Gly Val Ile Lys Ile Leu Thr Tyr Pro Trp Asp Arg Ile
2840 2845 2850
Glu Glu Val Thr Arg Met Ala Met Thr Asp Thr Thr Pro Phe Gly
2855 2860 2865
Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg Ala Lys Asp
2870 2875 2880
Pro Pro Ala Gly Thr Arg Lys Ile Met Lys Val Val Asn Arg Trp
2885 2890 2895
Leu Phe Arg His Leu Ala Arg Glu Lys Asn Pro Arg Leu Cys Thr
2900 2905 2910
Lys Glu Glu Phe Ile Ala Lys Val Arg Ser His Ala Ala Ile Gly
2915 2920 2925
Ala Tyr Leu Glu Glu Gln Glu Gln Trp Lys Thr Ala Asn Glu Ala
2930 2935 2940
Val Gln Asp Pro Lys Phe Trp Glu Leu Val Asp Glu Glu Arg Lys
2945 2950 2955
Leu His Gln Gln Gly Arg Cys Arg Thr Cys Val Tyr Asn Met Met
2960 2965 2970
Gly Lys Arg Glu Lys Lys Leu Ser Glu Phe Gly Lys Ala Lys Gly
2975 2980 2985
Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg Tyr Leu Glu
2990 2995 3000
Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp Ala Ser Arg
3005 3010 3015
Glu Asn Ser Gly Gly Gly Val Glu Gly Ile Gly Leu Gln Tyr Leu
3020 3025 3030
Gly Tyr Val Ile Arg Asp Leu Ala Ala Met Asp Gly Gly Gly Phe
3035 3040 3045
Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile Thr Glu Ala
3050 3055 3060
Asp Leu Asp Asp Glu Gln Glu Ile Leu Asn Tyr Met Ser Pro His
3065 3070 3075
His Lys Lys Leu Ala Gln Ala Val Met Glu Met Thr Tyr Lys Asn
3080 3085 3090
Lys Val Val Lys Val Leu Arg Pro Ala Pro Gly Gly Lys Ala Tyr
3095 3100 3105
Met Asp Val Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly Gln Val
3110 3115 3120
Val Thr Tyr Ala Leu Asn Thr Ile Thr Asn Leu Lys Val Gln Leu
3125 3130 3135
Ile Arg Met Ala Glu Ala Glu Met Val Ile His His Gln His Val
3140 3145 3150
Gln Asp Cys Asp Glu Ser Val Leu Thr Arg Leu Glu Ala Trp Leu
3155 3160 3165
Thr Glu His Gly Cys Asp Arg Leu Lys Arg Met Ala Val Ser Gly
3170 3175 3180
Asp Asp Cys Val Val Arg Pro Ile Asp Asp Arg Phe Gly Leu Ala
3185 3190 3195
Leu Ser His Leu Asn Ala Met Ser Lys Val Arg Lys Asp Ile Ser
3200 3205 3210
Glu Trp Gln Pro Ser Lys Gly Trp Asn Asp Trp Glu Asn Val Pro
3215 3220 3225
Phe Cys Ser His His Phe His Glu Leu Gln Leu Lys Asp Gly Arg
3230 3235 3240
Arg Ile Val Val Pro Cys Arg Glu Gln Asp Glu Leu Ile Gly Arg
3245 3250 3255
Gly Arg Val Ser Pro Gly Asn Gly Trp Met Ile Lys Glu Thr Ala
3260 3265 3270
Cys Leu Ser Lys Ala Tyr Ala Asn Met Trp Ser Leu Met Tyr Phe
3275 3280 3285
His Lys Arg Asp Met Arg Leu Leu Ser Leu Ala Val Ser Ser Ala
3290 3295 3300
Val Pro Thr Ser Trp Val Pro Gln Gly Arg Thr Thr Trp Ser Ile
3305 3310 3315
His Gly Lys Gly Glu Trp Met Thr Thr Glu Asp Met Leu Glu Val
3320 3325 3330
Trp Asn Arg Val Trp Ile Thr Asn Asn Pro His Met Gln Asp Lys
3335 3340 3345
Thr Met Val Lys Lys Trp Arg Asp Val Pro Tyr Leu Thr Lys Arg
3350 3355 3360
Gln Asp Lys Leu Cys Gly Ser Leu Ile Gly Met Thr Asn Arg Ala
3365 3370 3375
Thr Trp Ala Ser His Ile His Leu Val Ile His Arg Ile Arg Thr
3380 3385 3390
Leu Ile Gly Gln Glu Lys Tyr Thr Asp Tyr Leu Thr Val Met Asp
3395 3400 3405
Arg Tyr Ser Val Asp Ala Asp Leu Gln Leu Gly Glu Leu Ile
3410 3415 3420
<210>24
<211>10239
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和西尼罗病毒
<400>24
gtaaatcctg tgtgctaatt gaggtgcatt ggtctgcaaa tcgagttgct aggcaataaa 60
cacatttgga ttaattttaa tcgttcgttg agcgattagc agagaactga ccagaacatg 120
tctggtcgta aagctcaggg aaaaaccctg ggcgtcaata tggtacgacg aggagttcgc 180
tccttgtcaa acaaaataaa acaaaaaaca aaacaaattg gaaacagacc tggaccttca 240
agaggtgttc aaggatttat ctttttcttt ttgttcaaca ttttgactgg aaaaaagatc 300
acagcccacc taaagaggtt gtggaaaatg ctggacccaa gacaaggctt ggctgttcta 360
aggaaagtca agagagtggt ggccagtttg atgagaggat tgtcctcaag gaaacgccgt 420
tcccatgatg ttctgactgt gcaattccta attttgggaa tgctgttgat gacgggtgga 480
gttaccctct ctaacttcca agggaaggtg atgatgacgg taaatgctac tgacgtcaca 540
gatgtcatca cgattccaac agctgctgga aagaacctat gcattgtcag agcaatggat 600
gtgggataca tgtgcgatga tactatcact tatgaatgcc cagtgctgtc ggctggtaat 660
gatccagaag acatcgactg ttggtgcaca aagtcagcag tctacgtcag gtatggaaga 720
tgcaccaaga cacgccactc aagacgcagt cggaggtcac tgacagtgca gacacacgga 780
gaaagcactc tagcgaacaa gaagggggct tggatggaca gcaccaaggc cacaaggtat 840
ttggtaaaaa cagaatcatg gatcttgagg aaccctggat atgccctggt ggcagccgtc 900
attggttgga tgcttgggag caacaccatg cagagagttg tgtttgtcgt gctattgctt 960
ttggtggccc cagcttacag cttcaactgc cttggaatga gcaacagaga cttcttggaa 1020
ggagtgtctg gagcaacatg ggtggatttg gttctcgaag gcgacagctg cgtgactatc 1080
atgtctaagg acaagcctac catcgacgtc aagatgatga atatggaggc ggccaacctg 1140
gcagaggtcc gcagttattg ctatttggct accgtcagcg atctctccac caaagctgca 1200
tgcccgacca tgggagaagc tcacaatgac aaacgtgctg acccagcttt tgtgtgcaga 1260
caaggagtgg tggacagggg ctggggcaac ggctgcggat tttttggcaa aggatccatt 1320
gacacatgcg ccaaatttgc ctgctctacc aaggcaatag gaagaaccat cttgaaagag 1380
aatatcaagt acgaagtggc catttttgtc catggaccaa ctactgtgga gtcgcacgga 1440
aattactcca cacaggttgg agccactcag gccggccgat tcagcatcac tcctgctgcg 1500
ccttcataca cactaaagct tggagaatat ggagaggtga cagtggactg tgaaccacgg 1560
tcagggattg acaccaatgc atactacgtg atgactgttg gaacaaagac gttcttggtc 1620
catcgtgagt ggttcatgga cctcaacctc ccttggagca gtgctggaag tactgtgtgg 1680
aggaacagag agacgttaat ggagtttgag gaaccacacg ccacgaagca gtctgtgata 1740
gcattgggct cacaagaggg agctctgcat caagctttgg ctggagccat tcctgtggaa 1800
ttttcaagca acactgtcaa gttgacgtcg ggtcatttga agtgtagagt gaagatggaa 1860
aaattgcagt tgaagggaac aacctatggc gtctgttcaa aggctttcaa gtttcttagg 1920
actcccgtgg acaccggtca cggcactgtg gtgttggaat tgcagtacac tggcacggat 1980
ggaccttgca aagttcctat ctcgtcagtg gcttcattga acgacctaac gccagtgggc 2040
agattggtca ctgtcaaccc ttttgtttca gtggccacgg ccaacgctaa ggtcctgatt 2100
gaattggaac caccctttgg agactcatac atagtggtgg gcagaggaga acaacagatc 2160
aatcaccatt ggcacaagtc tggaagcagc attggcaaag cctttacaac caccctcaaa 2220
ggagcgcaga gactagccgc tctaggagac acagcttggg actttggatc agttggaggg 2280
gtgttcacta gtgttgggcg ggctgtccat caagtgttcg gaggagcatt ccgctcactg 2340
ttcggaggca tgtcctggat aacgcaagga ttgctggggg ctctcctgtt gtggatgggc 2400
atcaatgctc gtgataggtc catagctctc acgtttctcg cagttggagg agttctgctc 2460
ttcctctccg tgaacgtggg cgccgatcaa ggatgcgcca tcaactttgg caagagagag 2520
ctcaagtgcg gagatggtat cttcatattt agagactctg atgactggct gaacaagtac 2580
tcatactatc cagaagatcc tgtgaagctt gcatcaatag tgaaagcctc ttttgaagaa 2640
gggaagtgtg gcctaaattc agttgactcc cttgagcatg agatgtggag aagcagggca 2700
gatgagatca atgccatttt tgaggaaaac gaggtggaca tttctgttgt cgtgcaggat 2760
ccaaagaatg tttaccagag aggaactcat ccattttcca gaattcggga tggtctgcag 2820
tatggttgga agacttgggg taagaacctt gtgttctccc cagggaggaa gaatggaagc 2880
ttcatcatag atggaaagtc caggaaagaa tgcccgtttt caaaccgggt ctggaattct 2940
ttccagatag aggagtttgg gacgggagtg ttcaccacac gcgtgtacat ggacgcagtc 3000
tttgaataca ccatagactg cgatggatct atcttgggtg cagcggtgaa cggaaaaaag 3060
agtgcccatg gctctccaac attttggatg ggaagtcatg aagtaaatgg gacatggatg 3120
atccacacct tggaggcatt agattacaag gagtgtgagt ggccactgac acatacgatt 3180
ggaacatcag ttgaagagag tgaaatgttc atgccgagat caatcggagg cccagttagc 3240
tctcacaatc atatccctgg atacaaggtt cagacgaacg gaccttggat gcaggtacca 3300
ctagaagtga agagagaagc ttgcccaggg actagcgtga tcattgatgg caactgtgat 3360
ggacggggaa aatcaaccag atccaccacg gatagcggga aagttattcc tgaatggtgt 3420
tgccgctcct gcacaatgcc gcctgtgagc ttccatggta gtgatgggtg ttggtatccc 3480
atggaaatta ggccaaggaa aacgcatgaa agccatctgg tgcgctcctg ggttacagct 3540
ggagaaatac atgctgtccc ttttggtttg gtgagcatga tgatagcaat ggaagtggtc 3600
ctaaggaaaa gacagggacc aaagcaaatg ttggttggag gagtagtgct cttgggagca 3660
atgctggtcg ggcaagtaac tctccttgat ttgctgaaac tcacagtggc tgtgggattg 3720
catttccatg agatgaacaa tggaggagac gccatgtata tggcgttgat tgctgccttt 3780
tcaatcagac cagggctgct catcggcttt gggctcagga ccctatggag ccctcgggaa 3840
cgccttgtgc tgaccctagg agcagccatg gtggagattg ccttgggtgg cgtgatgggc 3900
ggcctgtgga agtatctaaa tgcagtttct ctctgcatcc tgacaataaa tgctgttgct 3960
tctaggaaag catcaaatac catcttgccc ctcatggctc tgttgacacc tgtcactatg 4020
gctgaggtga gacttgccgc aatgttcttt tgtgccatgg ttatcatagg ggtccttcac 4080
cagaatttca aggacacctc catgcagaag actatacctc tggtggccct cacactcaca 4140
tcttacctgg gcttgacaca accttttttg ggcctgtgtg catttctggc aacccgcata 4200
tttgggcgaa ggagtatccc agtgaatgag gcactcgcag cagctggtct agtgggagtg 4260
ctggcaggac tggcttttca ggagatggag aacttccttg gtccgattgc agttggagga 4320
ctcctgatga tgctggttag cgtggctggg agggtggatg ggctagagct caagaagctt 4380
ggtgaagttt catgggaaga ggaggcggag atcagcggga gttccgcccg ctatgatgtg 4440
gcactcagtg aacaagggga gttcaagctg ctttctgaag agaaagtgcc atgggaccag 4500
gttgtgatga cctcgctggc cttggttggg gctgccctcc atccatttgc tcttctgctg 4560
gtccttgctg ggtggctgtt tcatgtcagg ggagctagga gaagtgggga tgtcttgtgg 4620
gatattccca ctcctaagat catcgaggaa tgtgaacatc tggaggatgg gatttatggc 4680
atattccagt caaccttctt gggggcctcc cagcgaggag tgggagtggc acagggaggg 4740
gtgttccaca caatgtggca tgtcacaaga ggagctttcc ttgtcaggaa tggcaagaag 4800
ttgattccat cttgggcttc agtaaaggaa gaccttgtcg cctatggtgg ctcatggaag 4860
ttggaaggca gatgggatgg agaggaagag gtccagttga tcgcggctgt tccaggaaag 4920
aacgtggtca acgtccagac aaaaccgagc ttgttcaaag tgaggaatgg gggagaaatc 4980
ggggctgtcg ctcttgacta tccgagtggc acttcaggat ctcctattgt taacaggaac 5040
ggagaggtga ttgggctgta cggcaatggc atccttgtcg gtgacaactc cttcgtgtcc 5100
gccatatccc agactgaggt gaaggaagaa ggaaaggagg agctccaaga gatcccgaca 5160
atgctaaaga aaggaatgac aactgtcctt gattttcatc ctggagctgg gaagacaaga 5220
cgtttcctcc cacagatctt ggccgagtgc gcacggagac gcttgcgcac tcttgtgttg 5280
gcccccacca gggttgttct ttctgaaatg aaggaggctt ttcacggcct ggacgtgaaa 5340
ttccacacac aggctttttc cgctcacggc agcgggagag aagtcattga tgccatgtgc 5400
catgccaccc taacttacag gatgttggaa ccaactaggg ttgttaactg ggaagtgatc 5460
attatggatg aagcccattt tttggatcca gccagcatag ccgctagagg ttgggcagcg 5520
cacagagcta gggcaaatga aagtgcaaca atcttgatga cagccacacc gcctgggact 5580
agtgatgaat ttccacattc aaatggtgaa atagaagatg ttcaaacgga catacccagt 5640
gagccctgga acacagggca tgactggatc ctggctgaca aaaggcccac ggcatggttc 5700
cttccatcca tcagagctgc aaatgtcatg gctgcctctt tgcgtaaggc tggaaagagt 5760
gtggtggtcc tgaacaggaa aacctttgag agagaatacc ccacgataaa gcagaagaaa 5820
cctgacttta tattggccac tgacatagct gaaatgggag ccaacctttg cgtggagcga 5880
gtgctggatt gcaggacggc ttttaagcct gtgcttgtgg atgaagggag gaaggtggca 5940
ataaaagggc cacttcgtat ctccgcatcc tctgctgctc aaaggagggg gcgcattggg 6000
agaaatccca acagagatgg agactcatac tactattctg agcctacaag tgaaaataat 6060
gcccaccacg tctgctggtt ggaggcctca atgctcttgg acaacatgga ggtgaggggt 6120
ggaatggtcg ccccactcta tggcgttgaa ggaactaaaa caccagtttc ccctggtgaa 6180
atgagactga gggatgacca gaggaaagtc ttcagagaac tagtgaggaa ttgtgacctg 6240
cccgtttggc tttcgtggca agtggccaag gctggtttga agacgaatga tcgtaagtgg 6300
tgttttgaag gccctgagga acatgagatc ttgaatgaca gcggtgaaac agtgaagtgc 6360
agggctcctg gaggagcaaa gaagcctctg cgcccaaggt ggtgtgatga aagggtgtca 6420
tctgaccaga gtgcgctgtc tgaatttatt aagtttgctg aaggtaggag gggagctgct 6480
gaagtgctag ttgtgctgag tgaactccct gatttcctgg ctaaaaaagg tggagaggca 6540
atggatacca tcagtgtgtt cctccactct gaggaaggct ctagggctta ccgcaatgca 6600
ctatcaatga tgcctgaggc aatgacaata gtcatgctgt ttatactggc tggactactg 6660
acatcgggaa tggtcatctt tttcatgtct cccaaaggca tcagtagaat gtctatggcg 6720
atgggcacaa tggccggctg tggatatctc atgttccttg gaggcgtcaa acccactcac 6780
atctcctatg tcatgctcat attctttgtc ctgatggtgg ttgtgatccc cgagccaggg 6840
caacaaaggt ccatccaaga caaccaagtg gcatacctca ttattggcat cctgacgctg 6900
gtttcagcgg tggcagccaa cgagctaggc atgctggaga aaaccaaaga ggacctcttt 6960
gggaagaaga acttaattcc atctagtgct tcaccctgga gttggccgga tcttgacctg 7020
aagccaggag ctgcctggac agtgtacgtt ggcattgtta caatgctctc tccaatgttg 7080
caccactgga tcaaagtcga atatggcaac ctgtctctgt ctggaatagc ccagtcagcc 7140
tcagtccttt ctttcatgga caaggggata ccattcatga agatgaatat ctcggtcata 7200
atgctgctgg tcagtggctg gaattcaata acagtgatgc ctctgctctg tggcataggg 7260
tgcgccatgc tccactggtc tctcatttta cctggaatca aagcgcagca gtcaaagctt 7320
gcacagagaa gggtgttcca tggcgttgcc aagaaccctg tggttgatgg gaatccaaca 7380
gttgacattg aggaagctcc tgaaatgcct gccctttatg agaagaaact ggctctatat 7440
ctccttcttg ctctcagcct agcttctgtt gccatgtgca gaacgccctt ttcattggct 7500
gaaggcattg tcctagcatc agctgcctta gggccgctca tagagggaaa caccagcctt 7560
ctttggaatg gacccatggc tgtctccatg acaggagtca tgagggggaa tcactatgct 7620
tttgtgggag tcatgtacaa tctatggaag atgaaaactg gacgccgggg gagcgcgaat 7680
ggaaaaactt tgggtgaagt ctggaagagg gaactgaatc tgttggacaa gcgacagttt 7740
gagttgtata aaaggaccga cattgtggag gtggatcgtg atacggcacg caggcatttg 7800
gccgaaggga aggtggacac cggggtggcg gtctccaggg ggaccgcaaa gttaaggtgg 7860
ttccatgagc gtggctatgt caagctggaa ggtagggtga ttgacctggg gtgtggccgc 7920
ggaggctggt gttactacgc tgctgcgcaa aaggaagtga gtggggtcaa aggatttact 7980
cttggaagag acggccatga gaaacccatg aatgtgcaaa gtctgggatg gaacatcatc 8040
accttcaagg acaaaactga tatccaccgc ctagaaccag tgaaatgtga cacccttttg 8100
tgtgacattg gagagtcatc atcgtcatcg gtcacagagg gggaaaggac cgtgagagtt 8160
cttgatactg tagaaaaatg gctggcttgt ggggttgaca acttctgtgt gaaggtgtta 8220
gctccataca tgccagatgt tcttgagaaa ctggaattgc tccaaaggag gtttggcgga 8280
acagtgatca ggaaccctct ctccaggaat tccactcatg aaatgtacta cgtgtctgga 8340
gcccgcagca atgtcacatt tactgtgaac caaacatccc gcctcctgat gaggagaatg 8400
aggcgtccaa ctggaaaagt gaccctggag gctgacgtca tcctcccaat tgggacacgc 8460
agtgttgaga cagacaaggg acccctggac aaagaggcca tagaagaaag ggttgagagg 8520
ataaaatctg agtacatgac ctcttggttt tatgacaatg acaaccccta caggacctgg 8580
cactactgtg gctcctatgt cacaaaaacc tccggaagtg cggcgagcat ggtaaatggt 8640
gttattaaaa ttctgacata tccatgggac aggatagagg aggtcacaag aatggcaatg 8700
actgacacaa ccccttttgg acagcaaaga gtgtttaaag aaaaagttga caccagagca 8760
aaggatccac cagcgggaac taggaagatc atgaaagttg tcaacaggtg gctgttccgc 8820
cacctggcca gagaaaagaa ccccagactg tgcacaaagg aagaatttat tgcaaaagtc 8880
cgaagtcatg cagccattgg agcttacctg gaagaacaag aacagtggaa gactgccaat 8940
gaggctgtcc aagacccaaa gttctgggaa ctggtggatg aagaaaggaa gctgcaccaa 9000
caaggcaggt gtcggacttg tgtgtacaac atgatgggga aaagagagaa gaagctgtca 9060
gagtttggga aagcaaaggg aagccgtgcc atatggtata tgtggctggg agcgcggtat 9120
cttgagtttg aggccctggg attcctgaat gaggaccatt gggcttccag ggaaaactca 9180
ggaggaggag tggaaggcat tggcttacaa tacctaggat atgtgatcag agacctggct 9240
gcaatggatg gtggtggatt ctacgcggat gacaccgctg gatgggacac gcgcatcaca 9300
gaggcagacc ttgatgatga acaggagatc ttgaactaca tgagcccaca tcacaaaaaa 9360
ctggcacaag cagtgatgga aatgacatac aagaacaaag tggtgaaagt gttgagacca 9420
gccccaggag ggaaagccta catggatgtc ataagtcgac gagaccagag aggatccggg 9480
caggtagtga cttatgctct gaacaccatc accaacttga aagtccaatt gatcagaatg 9540
gcagaagcag agatggtgat acatcaccaa catgttcaag attgtgatga atcagttctg 9600
accaggctgg aggcatggct cactgagcac ggatgtgaca gactgaagag gatggcggtg 9660
agtggagacg actgtgtggt ccggcccatc gatgacaggt tcggcctggc cctgtcccat 9720
ctcaacgcca tgtccaaggt tagaaaggac atatctgaat ggcagccatc aaaagggtgg 9780
aatgattggg agaatgtgcc cttctgttcc caccacttcc atgaactaca gctgaaggat 9840
ggcaggagga ttgtggtgcc ttgccgagaa caggacgagc tcattgggag aggaagggtg 9900
tctccaggaa acggctggat gatcaaggaa acagcttgcc tcagcaaagc ctatgccaac 9960
atgtggtcac tgatgtattt tcacaaaagg gacatgaggc tactgtcatt ggctgtttcc 10020
tcagctgttc ccacctcatg ggttccacaa ggacgcacaa catggtcgat tcatgggaaa 10080
ggggagtgga tgaccacgga agacatgctt gaggtgtgga acagagtatg gataaccaac 10140
aacccacaca tgcaggacaa gacaatggtg aaaaaatgga gagatgtccc ttatctaacc 10200
aagagacaag acaagctgtg cggatcactg attggaatg 10239
<210>25
<211>12
<212>PRT
<213>人工序列
<220>
<223>衍生于西尼罗病毒
<400>25
Ser Leu Thr Val Gln Thr His Gly Glu Ser Thr Leu
1 5 10
<210>26
<211>12
<212>PRT
<213>人工序列
<220>
<223>衍生于西尼罗病毒
<400>26
Val Val Leu Leu Leu Leu Val Ala Pro Ala Tyr Ser
1 5 10
<210>27
<211>12
<212>PRT
<213>人工序列
<220>
<223>衍生于西尼罗病毒
<400>27
Val Val Pro Leu Leu Leu Val Ala Pro Ala Tyr Ser
1 5 10
<210>28
<211>75
<212>PRT
<213>人工序列
<220>
<223>衍生于西尼罗病毒
<400>28
Ser Leu Thr Val Gln Thr His Gly Glu Ser Thr Leu Ala Asn Lys Lys
1 5 10 15
Gly Ala Trp Met Asp Ser Thr Lys Ala Thr Arg Tyr Leu Val Lys Thr
20 25 30
Glu Ser Trp Ile Leu Arg Asn Pro Gly Tyr Ala Leu Val Ala Ala Val
35 40 45
Ile Gly Trp Met Leu Gly Ser Asn Thr Met Gln Arg Val Val Phe Val
50 55 60
Val Leu Leu Leu Leu Val Ala Pro Ala Tyr Ser
65 70 75
<210>29
<211>74
<212>PRT
<213>人工序列
<220>
<223>衍生于西尼罗病毒
<400>29
Ser Leu Thr Val Gln Thr His Gly Glu Ser Thr Leu Ala Asn Lys Lys
1 5 10 15
Gly Ala Trp Met Asp Ser Thr Lys Ala Thr Arg Tyr Leu Val Lys Thr
20 25 30
Glu Ser Trp Ile Leu Arg Asn Pro Gly Tyr Ala Leu Val Ala Ala Val
35 40 45
Ile Gly Trp Met Leu Gly Ser Asn Thr Met Gln Arg Val Val Phe Val
50 55 60
Val Leu Leu Leu Val Ala Pro Ala Tyr Ser
65 70
<210>30
<211>75
<212>PRT
<213>人工序列
<220>
<223>衍生于西尼罗病毒
<400>30
Ser Leu Thr Val Gln Thr His Gly Glu Ser Thr Leu Ala Asn Lys Lys
1 5 10 15
Gly Ala Trp Met Asp Ser Thr Lys Ala Thr Arg Tyr Leu Val Lys Thr
20 25 30
Glu Ser Trp Ile Leu Arg Asn Pro Gly Tyr Ala Leu Val Ala Ala Val
35 40 45
Ile Gly Trp Met Leu Gly Ser Asn Thr Met Gln Arg Val Val Phe Val
50 55 60
Val Pro Leu Leu Leu Val Ala Pro Ala Tyr Ser
65 70 75
<210>31
<211>377
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒
<400>31
taaaaactac ggatggagaa ccggactcca cacattgaga cagaagaagt tgtcagccca 60
gaaccccaca cgagttttgc cactgctaag ctgtgaggca gtgcaggctg ggacagccga 120
cctccaggtt gcgataaacc tggtttctgg gacctcccac cccagagtaa aaagaacgga 180
gcctccgcta ccaccttccc acgtggtggt agaaagacgg ggtctagagg ttagaggaga 240
ccctccaggg aacaaatagt gggaccatat tgacgccagg gaaagaccgg agtggttctc 300
tgcttttcct ccagaggtct gtgagcacag tttgctcaag aataagcaga cctttggatg 360
acaaacacaa aaccact 377
<210>32
<211>343
<212>RNA
<213>人工序列
<220>
<223>衍生于黄热病病毒
<400>32
uugagacaga agaaguuguc agcccagaac cccacacgag uuuugccacu gcuaagcugu 60
gaggcagugc aggcugggac agccgaccuc cagguugcga aaaaccuggu uucugggacc 120
ucccacccca gaguaaaaag aacggagccu ccgcuaccac ccucccacgu ggugguagaa 180
agacgggguc uagagguuag aggagacccu ccagggaaca aauaguggga ccauauugac 240
gccagggaaa gaccggagug guucucugcu uuuccuccag aggucuguga gcacaguuug 300
cucaagaaua agcagaccuu uggaugacaa acacaaaacc acu 343
<210>33
<211>338
<212>RNA
<213>人工序列
<220>
<223>衍生于黄热病病毒
<400>33
uugagacaga agaaguuguc agcccagaac cccacacgag uuuugccacu gcuaagcugu 60
gaggcagugc aggcugggac agccgaccuc cagguugcga aaaaccuggu uucugggacc 120
ucccaccgua aaaagaaggg agccucggcu accacccucc cacguggugg uagaaagacg 180
gggucuagag guuagaggag acccuccagg gaacaaauag ugggaccaua uugaggccag 240
ggaaagaccg gagugguucu cugcuuuucc uccagagguc ugugagcaca guuugcucaa 300
gaauaagcag accuuuggau gacaaacaga aaaccacu 338
<210>34
<211>116
<212>PRT
<213>人工序列
<220>
<223>衍生于蜱传脑炎病毒
<400>34
Met Val Lys Lys Ala Ile Leu Lys Gly Lys Gly Gly Gly Pro Pro Arg
1 5 10 15
Arg Val Ser Lys Glu Thr Ala Thr Lys Thr Arg Gln Pro Arg Val Gln
20 25 30
Met Pro Asn Gly Leu Val Leu Met Arg Met Met Gly Ile Leu Trp His
35 40 45
Ala Val Ala Gly Thr Ala Arg Asn Pro Val Leu Lys Ala Phe Trp Asn
50 55 60
Ser Val Pro Leu Lys Gln Ala Thr Ala Ala Leu Arg Lys Ile Lys Arg
65 70 75 80
Thr Val Ser Ala Leu Met Val Gly Leu Gln Lys Arg Gly Lys Arg Arg
85 90 95
Ser Ala Thr Asp Trp Met Ser Trp Leu Leu Val Ile Thr Leu Leu Gly
100 105 110
Met Thr Leu Ala
115
<210>35
<211>121
<212>PRT
<213>人工序列
<220>
<223>衍生于黄热病病毒
<400>35
Met Ser Gly Arg Lys Ala Gln Gly Lys Thr Leu Gly Val Asn Met Val
1 5 10 15
Arg Arg Gly Val Arg Ser Leu Ser Asn Lys Ile Lys Gln Lys Thr Lys
20 25 30
Gln Ile Gly Asn Arg Pro Gly Pro Ser Arg Gly Val Gln Gly Phe Ile
35 40 45
Phe Phe Phe Leu Phe Asn Ile Leu Thr Gly Lys Lys Ile Thr Ala His
50 55 60
Leu Lys Arg Leu Trp Lys Met Leu Asp Pro Arg Gln Gly Leu Ala Val
65 70 75 80
Leu Arg Lys Val Lys Arg Val Val Ala Ser Leu Met Arg Gly Leu Ser
85 90 95
Ser Arg Lys Arg Arg Ser His Asp Val Leu Thr Val Gln Phe Leu Ile
100 105 110
Leu Gly Met Leu Leu Met Thr Gly Gly
115 120
<210>36
<211>11
<212>DNA
<213>人工序列
<220>
<223>合成的构建体
<400>36
gtaaatcctg t 11
<210>37
<211>12
<212>DNA
<213>人工序列
<220>
<223>合成的构建体
<400>37
acaaaaccac aa 12
<210>38
<211>10239
<212>DNA
<213>人工序列
<220>
<223>衍生于黄热病病毒和西尼罗病毒
<400>38
gtaaatcctg tgtgctaatt gaggtgcatt ggtctgcaaa tcgagttgct aggcaataaa 60
cacatttgga ttaattttaa tcgttcgttg agcgattagc agagaactga ccagaacatg 120
tctggtcgta aagctcaggg aaaaaccctg ggcgtcaata tggtacgacg aggagttcgc 180
tccttgtcaa acaaaataaa acaaaaaaca aaacaaattg gaaacagacc tggaccttca 240
agaggtgttc aaggatttat ctttttcttt ttgttcaaca ttttgactgg aaaaaagatc 300
acagcccacc taaagaggtt gtggaaaatg ctggacccaa gacaaggctt ggctgttcta 360
aggaaagtca agagagtggt ggccagtttg atgagaggat tgtcctcaag gaaacgccgt 420
tcccatgatg ttctgactgt gcaattccta attttgggaa tgctgttgat gacgggtgga 480
gttaccctct ctaacttcca agggaaggtg atgatgacgg taaatgctac tgacgtcaca 540
gatgtcatca cgattccaac agctgctgga aagaacctat gcattgtcag agcaatggat 600
gtgggataca tgtgcgatga tactatcact tatgaatgcc cagtgctgtc ggctggtaat 660
gatccagaag acatcgactg ttggtgcaca aagtcagcag tctacgtcag gtatggaaga 720
tgcaccaaga cacgccactc aagacgcagt cggaggtcac tgacagtgca gacacacgga 780
gaaagcactc tagcgaacaa gaagggggct tggatggaca gcaccaaggc cacaaggtat 840
ttggtaaaaa cagaatcatg gatcttgagg aaccctggat atgccctggt ggcagccgtc 900
attggttgga tgcttgggag caacaccatg cagagagttg tgtttgtcgt gccattgctt 960
ttggtggccc cagcttacag cttcaactgc cttggaatga gcaacagaga cttcttggaa 1020
ggagtgtctg gagcaacatg ggtggatttg gttctcgaag gcgacagctg cgtgactatc 1080
atgtctaagg acaagcctac catcgacgtc aagatgatga atatggaggc ggccaacctg 1140
gcagaggtcc gcagttattg ctatttggct accgtcagcg atctctccac caaagctgca 1200
tgcccgacca tgggagaagc tcacaatgac aaacgtgctg acccagcttt tgtgtgcaga 1260
caaggagtgg tggacagggg ctggggcaac ggctgcggat tttttggcaa aggatccatt 1320
gacacatgcg ccaaatttgc ctgctctacc aaggcaatag gaagaaccat cttgaaagag 1380
aatatcaagt acgaagtggc catttttgtc catggaccaa ctactgtgga gtcgcacgga 1440
aattactcca cacaggttgg agccactcag gccggccgat tcagcatcac tcctgctgcg 1500
ccttcataca cactaaagct tggagaatat ggagaggtga cagtggactg tgaaccacgg 1560
tcagggattg acaccaatgc atactacgtg atgactgttg gaacaaagac gttcttggtc 1620
catcgtgagt ggttcatgga cctcaacctc ccttggagca gtgctggaag tactgtgtgg 1680
aggaacagag agacgttaat ggagtttgag gaaccacacg ccacgaagca gtctgtgata 1740
gcattgggct cacaagaggg agctctgcat caagctttgg ctggagccat tcctgtggaa 1800
ttttcaagca acactgtcaa gttgacgtcg ggtcatttga agtgtagagt gaagatggaa 1860
aaattgcagt tgaagggaac aacctatggc gtctgttcaa aggctttcaa gtttcttagg 1920
actcccgtgg acaccggtca cggcactgtg gtgttggaat tgcagtacac tggcacggat 1980
ggaccttgca aagttcctat ctcgtcagtg gcttcattga acgacctaac gccagtgggc 2040
agattggtca ctgtcaaccc ttttgtttca gtggccacgg ccaacgctaa ggtcctgatt 2100
gaattggaac caccctttgg agactcatac atagtggtgg gcagaggaga acaacagatc 2160
aatcaccatt ggcacaagtc tggaagcagc attggcaaag cctttacaac caccctcaaa 2220
ggagcgcaga gactagccgc tctaggagac acagcttggg actttggatc agttggaggg 2280
gtgttcacta gtgttgggcg ggctgtccat caagtgttcg gaggagcatt ccgctcactg 2340
ttcggaggca tgtcctggat aacgcaagga ttgctggggg ctctcctgtt gtggatgggc 2400
atcaatgctc gtgataggtc catagctctc acgtttctcg cagttggagg agttctgctc 2460
ttcctctccg tgaacgtggg cgccgatcaa ggatgcgcca tcaactttgg caagagagag 2520
ctcaagtgcg gagatggtat cttcatattt agagactctg atgactggct gaacaagtac 2580
tcatactatc cagaagatcc tgtgaagctt gcatcaatag tgaaagcctc ttttgaagaa 2640
gggaagtgtg gcctaaattc agttgactcc cttgagcatg agatgtggag aagcagggca 2700
gatgagatca atgccatttt tgaggaaaac gaggtggaca tttctgttgt cgtgcaggat 2760
ccaaagaatg tttaccagag aggaactcat ccattttcca gaattcggga tggtctgcag 2820
tatggttgga agacttgggg taagaacctt gtgttctccc cagggaggaa gaatggaagc 2880
ttcatcatag atggaaagtc caggaaagaa tgcccgtttt caaaccgggt ctggaattct 2940
ttccagatag aggagtttgg gacgggagtg ttcaccacac gcgtgtacat ggacgcagtc 3000
tttgaataca ccatagactg cgatggatct atcttgggtg cagcggtgaa cggaaaaaag 3060
agtgcccatg gctctccaac attttggatg ggaagtcatg aagtaaatgg gacatggatg 3120
atccacacct tggaggcatt agattacaag gagtgtgagt ggccactgac acatacgatt 3180
ggaacatcag ttgaagagag tgaaatgttc atgccgagat caatcggagg cccagttagc 3240
tctcacaatc atatccctgg atacaaggtt cagacgaacg gaccttggat gcaggtacca 3300
ctagaagtga agagagaagc ttgcccaggg actagcgtga tcattgatgg caactgtgat 3360
ggacggggaa aatcaaccag atccaccacg gatagcggga aagttattcc tgaatggtgt 3420
tgccgctcct gcacaatgcc gcctgtgagc ttccatggta gtgatgggtg ttggtatccc 3480
atggaaatta ggccaaggaa aacgcatgaa agccatctgg tgcgctcctg ggttacagct 3540
ggagaaatac atgctgtccc ttttggtttg gtgagcatga tgatagcaat ggaagtggtc 3600
ctaaggaaaa gacagggacc aaagcaaatg ttggttggag gagtagtgct cttgggagca 3660
atgctggtcg ggcaagtaac tctccttgat ttgctgaaac tcacagtggc tgtgggattg 3720
catttccatg agatgaacaa tggaggagac gccatgtata tggcgttgat tgctgccttt 3780
tcaatcagac cagggctgct catcggcttt gggctcagga ccctatggag ccctcgggaa 3840
cgccttgtgc tgaccctagg agcagccatg gtggagattg ccttgggtgg cgtgatgggc 3900
ggcctgtgga agtatctaaa tgcagtttct ctctgcatcc tgacaataaa tgctgttgct 3960
tctaggaaag catcaaatac catcttgccc ctcatggctc tgttgacacc tgtcactatg 4020
gctgaggtga gacttgccgc aatgttcttt tgtgccatgg ttatcatagg ggtccttcac 4080
cagaatttca aggacacctc catgcagaag actatacctc tggtggccct cacactcaca 4140
tcttacctgg gcttgacaca accttttttg ggcctgtgtg catttctggc aacccgcata 4200
tttgggcgaa ggagtatccc agtgaatgag gcactcgcag cagctggtct agtgggagtg 4260
ctggcaggac tggcttttca ggagatggag aacttccttg gtccgattgc agttggagga 4320
ctcctgatga tgctggttag cgtggctggg agggtggatg ggctagagct caagaagctt 4380
ggtgaagttt catgggaaga ggaggcggag atcagcggga gttccgcccg ctatgatgtg 4440
gcactcagtg aacaagggga gttcaagctg ctttctgaag agaaagtgcc atgggaccag 4500
gttgtgatga cctcgctggc cttggttggg gctgccctcc atccatttgc tcttctgctg 4560
gtccttgctg ggtggctgtt tcatgtcagg ggagctagga gaagtgggga tgtcttgtgg 4620
gatattccca ctcctaagat catcgaggaa tgtgaacatc tggaggatgg gatttatggc 4680
atattccagt caaccttctt gggggcctcc cagcgaggag tgggagtggc acagggaggg 4740
gtgttccaca caatgtggca tgtcacaaga ggagctttcc ttgtcaggaa tggcaagaag 4800
ttgattccat cttgggcttc agtaaaggaa gaccttgtcg cctatggtgg ctcatggaag 4860
ttggaaggca gatgggatgg agaggaagag gtccagttga tcgcggctgt tccaggaaag 4920
aacgtggtca acgtccagac aaaaccgagc ttgttcaaag tgaggaatgg gggagaaatc 4980
ggggctgtcg ctcttgacta tccgagtggc acttcaggat ctcctattgt taacaggaac 5040
ggagaggtga ttgggctgta cggcaatggc atccttgtcg gtgacaactc cttcgtgtcc 5100
gccatatccc agactgaggt gaaggaagaa ggaaaggagg agctccaaga gatcccgaca 5160
atgctaaaga aaggaatgac aactgtcctt gattttcatc ctggagctgg gaagacaaga 5220
cgtttcctcc cacagatctt ggccgagtgc gcacggagac gcttgcgcac tcttgtgttg 5280
gcccccacca gggttgttct ttctgaaatg aaggaggctt ttcacggcct ggacgtgaaa 5340
ttccacacac aggctttttc cgctcacggc agcgggagag aagtcattga tgccatgtgc 5400
catgccaccc taacttacag gatgttggaa ccaactaggg ttgttaactg ggaagtgatc 5460
attatggatg aagcccattt tttggatcca gccagcatag ccgctagagg ttgggcagcg 5520
cacagagcta gggcaaatga aagtgcaaca atcttgatga cagccacacc gcctgggact 5580
agtgatgaat ttccacattc aaatggtgaa atagaagatg ttcaaacgga catacccagt 5640
gagccctgga acacagggca tgactggatc ctggctgaca aaaggcccac ggcatggttc 5700
cttccatcca tcagagctgc aaatgtcatg gctgcctctt tgcgtaaggc tggaaagagt 5760
gtggtggtcc tgaacaggaa aacctttgag agagaatacc ccacgataaa gcagaagaaa 5820
cctgacttta tattggccac tgacatagct gaaatgggag ccaacctttg cgtggagcga 5880
gtgctggatt gcaggacggc ttttaagcct gtgcttgtgg atgaagggag gaaggtggca 5940
ataaaagggc cacttcgtat ctccgcatcc tctgctgctc aaaggagggg gcgcattggg 6000
agaaatccca acagagatgg agactcatac tactattctg agcctacaag tgaaaataat 6060
gcccaccacg tctgctggtt ggaggcctca atgctcttgg acaacatgga ggtgaggggt 6120
ggaatggtcg ccccactcta tggcgttgaa ggaactaaaa caccagtttc ccctggtgaa 6180
atgagactga gggatgacca gaggaaagtc ttcagagaac tagtgaggaa ttgtgacctg 6240
cccgtttggc tttcgtggca agtggccaag gctggtttga agacgaatga tcgtaagtgg 6300
tgttttgaag gccctgagga acatgagatc ttgaatgaca gcggtgaaac agtgaagtgc 6360
agggctcctg gaggagcaaa gaagcctctg cgcccaaggt ggtgtgatga aagggtgtca 6420
tctgaccaga gtgcgctgtc tgaatttatt aagtttgctg aaggtaggag gggagctgct 6480
gaagtgctag ttgtgctgag tgaactccct gatttcctgg ctaaaaaagg tggagaggca 6540
atggatacca tcagtgtgtt cctccactct gaggaaggct ctagggctta ccgcaatgca 6600
ctatcaatga tgcctgaggc aatgacaata gtcatgctgt ttatactggc tggactactg 6660
acatcgggaa tggtcatctt tttcatgtct cccaaaggca tcagtagaat gtctatggcg 6720
atgggcacaa tggccggctg tggatatctc atgttccttg gaggcgtcaa acccactcac 6780
atctcctatg tcatgctcat attctttgtc ctgatggtgg ttgtgatccc cgagccaggg 6840
caacaaaggt ccatccaaga caaccaagtg gcatacctca ttattggcat cctgacgctg 6900
gtttcagcgg tggcagccaa cgagctaggc atgctggaga aaaccaaaga ggacctcttt 6960
gggaagaaga acttaattcc atctagtgct tcaccctgga gttggccgga tcttgacctg 7020
aagccaggag ctgcctggac agtgtacgtt ggcattgtta caatgctctc tccaatgttg 7080
caccactgga tcaaagtcga atatggcaac ctgtctctgt ctggaatagc ccagtcagcc 7140
tcagtccttt ctttcatgga caaggggata ccattcatga agatgaatat ctcggtcata 7200
atgctgctgg tcagtggctg gaattcaata acagtgatgc ctctgctctg tggcataggg 7260
tgcgccatgc tccactggtc tctcatttta cctggaatca aagcgcagca gtcaaagctt 7320
gcacagagaa gggtgttcca tggcgttgcc aagaaccctg tggttgatgg gaatccaaca 7380
gttgacattg aggaagctcc tgaaatgcct gccctttatg agaagaaact ggctctatat 7440
ctccttcttg ctctcagcct agcttctgtt gccatgtgca gaacgccctt ttcattggct 7500
gaaggcattg tcctagcatc agctgcctta gggccgctca tagagggaaa caccagcctt 7560
ctttggaatg gacccatggc tgtctccatg acaggagtca tgagggggaa tcactatgct 7620
tttgtgggag tcatgtacaa tctatggaag atgaaaactg gacgccgggg gagcgcgaat 7680
ggaaaaactt tgggtgaagt ctggaagagg gaactgaatc tgttggacaa gcgacagttt 7740
gagttgtata aaaggaccga cattgtggag gtggatcgtg atacggcacg caggcatttg 7800
gccgaaggga aggtggacac cggggtggcg gtctccaggg ggaccgcaaa gttaaggtgg 7860
ttccatgagc gtggctatgt caagctggaa ggtagggtga ttgacctggg gtgtggccgc 7920
ggaggctggt gttactacgc tgctgcgcaa aaggaagtga gtggggtcaa aggatttact 7980
cttggaagag acggccatga gaaacccatg aatgtgcaaa gtctgggatg gaacatcatc 8040
accttcaagg acaaaactga tatccaccgc ctagaaccag tgaaatgtga cacccttttg 8100
tgtgacattg gagagtcatc atcgtcatcg gtcacagagg gggaaaggac cgtgagagtt 8160
cttgatactg tagaaaaatg gctggcttgt ggggttgaca acttctgtgt gaaggtgtta 8220
gctccataca tgccagatgt tcttgagaaa ctggaattgc tccaaaggag gtttggcgga 8280
acagtgatca ggaaccctct ctccaggaat tccactcatg aaatgtacta cgtgtctgga 8340
gcccgcagca atgtcacatt tactgtgaac caaacatccc gcctcctgat gaggagaatg 8400
aggcgtccaa ctggaaaagt gaccctggag gctgacgtca tcctcccaat tgggacacgc 8460
agtgttgaga cagacaaggg acccctggac aaagaggcca tagaagaaag ggttgagagg 8520
ataaaatctg agtacatgac ctcttggttt tatgacaatg acaaccccta caggacctgg 8580
cactactgtg gctcctatgt cacaaaaacc tccggaagtg cggcgagcat ggtaaatggt 8640
gttattaaaa ttctgacata tccatgggac aggatagagg aggtcacaag aatggcaatg 8700
actgacacaa ccccttttgg acagcaaaga gtgtttaaag aaaaagttga caccagagca 8760
aaggatccac cagcgggaac taggaagatc atgaaagttg tcaacaggtg gctgttccgc 8820
cacctggcca gagaaaagaa ccccagactg tgcacaaagg aagaatttat tgcaaaagtc 8880
cgaagtcatg cagccattgg agcttacctg gaagaacaag aacagtggaa gactgccaat 8940
gaggctgtcc aagacccaaa gttctgggaa ctggtggatg aagaaaggaa gctgcaccaa 9000
caaggcaggt gtcggacttg tgtgtacaac atgatgggga aaagagagaa gaagctgtca 9060
gagtttggga aagcaaaggg aagccgtgcc atatggtata tgtggctggg agcgcggtat 9120
cttgagtttg aggccctggg attcctgaat gaggaccatt gggcttccag ggaaaactca 9180
ggaggaggag tggaaggcat tggcttacaa tacctaggat atgtgatcag agacctggct 9240
gcaatggatg gtggtggatt ctacgcggat gacaccgctg gatgggacac gcgcatcaca 9300
gaggcagacc ttgatgatga acaggagatc ttgaactaca tgagcccaca tcacaaaaaa 9360
ctggcacaag cagtgatgga aatgacatac aagaacaaag tggtgaaagt gttgagacca 9420
gccccaggag ggaaagccta catggatgtc ataagtcgac gagaccagag aggatccggg 9480
caggtagtga cttatgctct gaacaccatc accaacttga aagtccaatt gatcagaatg 9540
gcagaagcag agatggtgat acatcaccaa catgttcaag attgtgatga atcagttctg 9600
accaggctgg aggcatggct cactgagcac ggatgtgaca gactgaagag gatggcggtg 9660
agtggagacg actgtgtggt ccggcccatc gatgacaggt tcggcctggc cctgtcccat 9720
ctcaacgcca tgtccaaggt tagaaaggac atatctgaat ggcagccatc aaaagggtgg 9780
aatgattggg agaatgtgcc cttctgttcc caccacttcc atgaactaca gctgaaggat 9840
ggcaggagga ttgtggtgcc ttgccgagaa caggacgagc tcattgggag aggaagggtg 9900
tctccaggaa acggctggat gatcaaggaa acagcttgcc tcagcaaagc ctatgccaac 9960
atgtggtcac tgatgtattt tcacaaaagg gacatgaggc tactgtcatt ggctgtttcc 10020
tcagctgttc ccacctcatg ggttccacaa ggacgcacaa catggtcgat tcatgggaaa 10080
ggggagtgga tgaccacgga agacatgctt gaggtgtgga acagagtatg gataaccaac 10140
aacccacaca tgcaggacaa gacaatggtg aaaaaatgga gagatgtccc ttatctaacc 10200
aagagacaag acaagctgtg cggatcactg attggaatg 10239