CN101082053B - Biofermentation method of foodstuff garbage - Google Patents
Biofermentation method of foodstuff garbage Download PDFInfo
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- CN101082053B CN101082053B CN200710072170A CN200710072170A CN101082053B CN 101082053 B CN101082053 B CN 101082053B CN 200710072170 A CN200710072170 A CN 200710072170A CN 200710072170 A CN200710072170 A CN 200710072170A CN 101082053 B CN101082053 B CN 101082053B
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- rubbish
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- food garbage
- aspergillus niger
- lactic acid
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Abstract
The biological food refuse fermenting process includes the following steps: 1. filtering to dry food refuse and inoculating aspergillus niger to ferment for preparing amylase; 2. adding the amylase and biofermenting bacteria to new food refuse to ferment; and 3. separating and purifying to obtain fermented product. The process has saving in resource, high food refuse utilizing rate, high yield of fermented product and low production cost.
Description
Technical field
The present invention relates to a kind of biofermentation method of rubbish.
Background technology
Food garbage refers to the changing food waste of dining room, dining room, family and the organic waste in the foodstuffs industry.Food garbage is a biomass waste, and the organic content height mainly comprises starch, Mierocrystalline cellulose, protein etc., can be used as biomass energy to utilize once again, and occupy larger specific gravity in domestic waste.The resource utilization of food garbage has been subjected to the great attention of scientific research scholar and government department, and fermentation and acid, alcohol, hydrogen, methane are the main modes of food garbage resource utilization.Because food garbage contains biomacromolecules such as protein, fat, polyose, be difficult to directly effectively be utilized by product fermentative production bacterial strain (as milk-acid bacteria, methanobacteria etc.); Utilize the organic acid tunning of food garbage to be raw material biosynthesizing polyhydroxyalkanoate (Polyhydroxyalkanoate as people such as U.S. GUOCHENG DU, PHA), organic acid concentration can only reach acetate 5.5g/L, propionic acid 1.8g/L, butyric acid 7.4g/L, lactic acid 32.7g/L (Environ.Sci.Technol.2002 (36): 5511-5516) behind 16 days anaerobically fermentings; People such as Japan KENJI SAKAI utilize the food garbage fermenting lactic acid, and lactic acid concn is also less than 30g/L (Journal Of BioscienceAnd Bioengineering.98 (1): 48-56) behind the fermentation 48h.Though add the output that the commercial enzyme preparation can improve target product in zymotechnique, production cost increases.
Summary of the invention
The objective of the invention is to be difficult to the defective directly effectively being utilized and use commercial enzyme preparation production cost to raise by product fermentative production bacterial strain in order to solve biomacromolecules such as protein in the present food garbage, fat, polyose, and the biofermentation method of a kind of food garbage that provides.
Food garbage is biological fermentation according to the following steps: (one) filters stem grafting kind aspergillus niger with food garbage, afterwards solid state fermentation preparation in 5～6 days saccharifying enzyme song under 30～35 ℃ condition; (2) saccharifying enzyme song and biological fermentation bacterial classification are joined in the fresh food garbage, and the fermentation of the tap water of quality such as adding and fresh provisions rubbish; (3) separate, purify, promptly get tunning.
Present embodiment can be used for fermentation production of organic acid, alcohol, hydrogen and methane, and can select biological fermentation bacterial classification and fermentation condition according to needed tunning, and the separation of tunning, method of purification can be adopted existing ordinary method.
The saccharifying enzyme Qu Bubi that the present invention prepares is dry and freezing, can directly use, and has saved a large amount of resources and has reduced production cost.Aspergillus niger in the saccharifying enzyme song of the present invention is because of without the dry and freezing high activity that kept, continued growth during the fermentation, product enzyme, and with fermented bacterium synergy, improved the utilization ratio of food garbage resource and the output of tunning greatly, can obviously reduce production costs.
Description of drawings
Fig. 1 is the saccharifying enzyme koji fermentation lactic acid and the production pattern that uses commercial enzyme preparation fermentation lactic acid that utilizes the aspergillus niger preparation in the embodiment 16, " zero " curve is to use the yield curve of commercial enzyme preparation fermentation lactic acid among Fig. 1, and " ■ " curve is the yield curve that utilizes the saccharifying enzyme koji fermentation lactic acid of aspergillus niger preparation among the figure; Fig. 2 is the bacterium discharge curve figure in aspergillus niger and the milk-acid bacteria cooperative fermentation process in the saccharifying enzyme song in the embodiment 16, and " zero " curve is a milk-acid bacteria bacterium discharge curve among Fig. 2, and " ■ " curve is an aspergillus niger spore quantity curve among Fig. 2.
Embodiment
Embodiment one: the present embodiment food garbage is biological fermentation according to the following steps: (one) filters stem grafting kind aspergillus niger with food garbage, afterwards solid state fermentation preparation in 5～6 days saccharifying enzyme song under 30～35 ℃ condition; (2) saccharifying enzyme song and biological fermentation bacterial classification are joined in the fresh food garbage, and the fermentation of the tap water of quality such as adding and fresh provisions rubbish; (3) separate, purify, promptly get tunning.
The saccharifying enzyme Qu Buxu drying that present embodiment step () is prepared can directly be used.
Present embodiment can be used for fermentation production of organic acid, alcohol, hydrogen and methane, and can select biological fermentation bacterial classification and fermentation condition according to needed tunning, and the separation of tunning, method of purification can be adopted existing ordinary method.
Embodiment two: the difference of present embodiment and embodiment one is: be ground into volume earlier less than 1.5cm after in the step () food garbage filter being done
3Particle.Other step and parameter are identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one is: 7%～9% inoculum density by the dried back of filter food garbage quality in the step () is 10
6～10
7The aspergillus niger spore suspension of spore/mL.Other step and parameter are identical with embodiment one.
Bent undried of saccharifying enzyme and purification that present embodiment is prepared, its glucoamylase enzyme work is 3500～4500U/g.
Embodiment four: present embodiment and embodiment one or threes' difference is: the aspergillus niger of inoculation is Aspergillus niger UV 448 in the step ().Other step and parameter are identical with embodiment one or three.
Present embodiment aspergillus niger Aspergillus niger UV 448 is available from Heilongjiang Province microorganism edible mushrooms institute.
Embodiment five: the difference of present embodiment and embodiment one is: 8% inoculum density by the dried back of filter food garbage quality in the step () is 10
6～10
7The aspergillus niger spore suspension of spore/mL.Other step and parameter are identical with embodiment one.
Embodiment six: the difference of present embodiment and embodiment one is: press 2%～3% of fresh provisions rubbish quality in the step (two) and add the saccharifying enzyme song, and add 6%～9% adding lactobacillus-fermented lactic acid of water secondary fermentation liquid total mass by food garbage.Other step and parameter are identical with embodiment one.
Embodiment seven: the difference of present embodiment and embodiment one is: press 2.5% of fresh provisions rubbish quality in the step (two) and add the saccharifying enzyme song, and add 7%～8% adding lactobacillus-fermented lactic acid of water secondary fermentation liquid total mass by food garbage.Other step and parameter are identical with embodiment one.
Embodiment eight: present embodiment and embodiment six or sevens' difference is: fresh provisions rubbish adds behind saccharifying enzyme song, milk-acid bacteria and the tap water condition bottom fermentation 25 ± 1h at 42～46 ℃ in the step (two).Other step and parameter are identical with embodiment six or seven.
Embodiment nine: the difference of present embodiment and embodiment one is: the water ratio of fresh provisions rubbish is 75%～85% in the step (two).Other step and parameter are identical with embodiment one.
Embodiment ten: the difference of present embodiment and embodiment one is: the water ratio of fresh provisions rubbish is 78%～83% in the step (two).Other step and parameter are identical with embodiment one.
Embodiment 11: the difference of present embodiment and embodiment one is: the water ratio of fresh provisions rubbish is 80% in the step (two).Other step and parameter are identical with embodiment one.
Embodiment 12: present embodiment and embodiment six or sevens' difference is: the milk-acid bacteria in the step (two) is Lactobacillus TD175.Other step and parameter are identical with embodiment six or seven.
Lactic bacterium strains Lactobacillus TD175 is milk-acid bacteria (the 1.Qunhui Wang that separates and identify from changing food waste in the present embodiment, Xuming Wang, et al.Bioconversion of KitchenGarbage to Lactic Acid by Two Wild Strains of Lactobacillus Species.Journal ofEnvironmental Science and Health, 40:1951-1962,2005; 2. Wang Xu is bright, Wang Qunhui etc. the isolation identification of a plant height lactic acid producing bacteria and leavening property research. and Harbin Institute of Technology's journal, 2006 (9): 1483-1486).
Embodiment 13: the difference of present embodiment and embodiment one is: in the step () behind the food garbage filter stem grafting kind aspergillus niger under 31～34 ℃ condition solid state fermentation 5 days, and stir once every 11～13h.Other step and parameter are identical with embodiment one.
Embodiment 14: the difference of present embodiment and embodiment one is: in the step () behind the food garbage filter stem grafting kind aspergillus niger under 32～33 ℃ condition solid state fermentation 5 days, and stir once every 12h.Other step and parameter are identical with embodiment one.
Embodiment 15: the difference of present embodiment and embodiment one is: the solids in step (two) the fresh provisions rubbish is pulverized is the particle of particle diameter less than 0.1cm.Other step and parameter are identical with embodiment one.
Embodiment 16: the present embodiment food garbage is biological fermentation lactic acid according to the following steps: (one) is ground into volume earlier less than 1.5cm after the food garbage filter is done
3Particle, 8% inoculum density by filter dried food rubbish quality is 10 again
6～10
7The aspergillus niger spore suspension of spore/mL, solid state fermentation 5 days under 32 ℃ condition then, and stir once preparation saccharifying enzyme song every 12h; (2) earlier solids in the fresh provisions rubbish being pulverized is the particle of particle diameter less than 0.1cm, and the tap water of quality such as adding and fresh provisions rubbish, add the saccharifying enzyme song, add 7%～8% of water secondary fermentation liquid total mass by food garbage and add lactobacillus-fermented lactic acid by 2.5% of fresh provisions rubbish quality again, and then at 45 ℃ condition bottom fermentation 25h; (3) lactic acid in the employing electroosmose process purification fermented liquid after the solid-liquid separation promptly gets lactic acid; Wherein said aspergillus niger is Aspergillus niger UV448; Milk-acid bacteria is Lactobacillus TD 175; The water ratio of fresh provisions rubbish is 75%～85% in the step (two).
Aspergillus niger can maintain high activity in the present embodiment saccharifying enzyme song, can continued growth in lactic fermentation process, produce enzyme, produce synergy as accompanying drawing 2 with milk-acid bacteria, saccharification result obviously is better than using merely the commercial enzyme preparation, more can impel the fermentation of lactic acid.Present embodiment fresh provisions rubbish detects lactic acid concn after solid-liquid separation can reach 63g/L.The saccharifying enzyme Qu Buxu drying of present embodiment preparation can directly be used.
The contrast experiment:
With the commercial enzyme preparation (saccharifying enzyme of fresh provisions rubbish quality 1%, glucoamylase enzyme is lived and is 100000U/g) replace the saccharifying enzyme Qu Jinhang lactic fermentation in the present embodiment, (two) are identical with present embodiment (embodiment 16) step for other fermentation condition; Detecting lactic acid concn through commercial fermentation using enzyme fresh provisions rubbish after solid-liquid separation is 49g/L.
Utilize aspergillus niger saccharifying enzyme koji fermentation lactic acid for preparing and the output of using commercial enzyme preparation fermentation lactic acid as shown in Figure 1, present embodiment utilizes the rate ratio of the saccharifying enzyme koji fermentation lactic acid of aspergillus niger preparation to use commercial enzyme preparation fermentation lactic acid high by 28.6%, has promoted the biological fermentation of food garbage.
The commercial enzyme preparation is available from Zhaodong north zymin factory among the contrast experiment.
Claims (1)
1. the biofermentation method of a food garbage is characterized in that food garbage biological fermentation according to the following steps: (one) is ground into volume earlier less than 1.5cm after the food garbage filter is done
3Particle, 8% inoculum density by filter dried food rubbish quality is 10 again
6～10
7The aspergillus niger spore suspension of spore/mL, solid state fermentation 5 days under 32 ℃ condition then, and stir once preparation saccharifying enzyme song every 12h; (2) earlier solids in the fresh provisions rubbish being pulverized is the particle of particle diameter less than 0.1cm, and the tap water of quality such as adding and fresh provisions rubbish, add the saccharifying enzyme song, add 7%～8% of water secondary fermentation liquid total mass by food garbage and add lactobacillus-fermented lactic acid by 2.5% of fresh provisions rubbish quality again, and then at 45 ℃ condition bottom fermentation 25h; (3) lactic acid in the employing electroosmose process purification fermented liquid after the solid-liquid separation promptly gets lactic acid; Wherein said aspergillus niger is Aspergillus niger UV448; Milk-acid bacteria is Lactobacillus TD175; The water ratio of fresh provisions rubbish is 75%～85% in the step (two).
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| CN200710072170A CN101082053B (en) | 2007-04-30 | 2007-04-30 | Biofermentation method of foodstuff garbage |
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| CN200710072170A CN101082053B (en) | 2007-04-30 | 2007-04-30 | Biofermentation method of foodstuff garbage |
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| CN101082053B true CN101082053B (en) | 2010-05-26 |
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| CN105618462A (en) * | 2016-01-18 | 2016-06-01 | 董艺 | Multipurpose kitchen garbage treatment system |
| CN117660203A (en) * | 2023-12-18 | 2024-03-08 | 河北省科学院生物研究所 | Anaerobic fermentation promoter and preparation and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843452A (en) * | 2006-02-16 | 2006-10-11 | 张高红 | Plaster for relieving asthma and stopping cough and its preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1843452A (en) * | 2006-02-16 | 2006-10-11 | 张高红 | Plaster for relieving asthma and stopping cough and its preparation method |
Non-Patent Citations (4)
| Title |
|---|
| 樊华等.稻草秸秆发酵同时糖化酵解产乳酸的初步研究.江西化工 1.2007,(1),第99页材料和方法部分-第102页结论部分. |
| 樊华等.稻草秸秆发酵同时糖化酵解产乳酸的初步研究.江西化工 1.2007,(1),第99页材料和方法部分-第102页结论部分. * |
| 王旭明等.高产乳酸细菌发酵厨余垃圾生产乳酸的试验研究.现代化工 S1.2005,(1),第148页材料于方法部分-第150页结语部分. |
| 王旭明等.高产乳酸细菌发酵厨余垃圾生产乳酸的试验研究.现代化工 S1.2005,(1),第148页材料于方法部分-第150页结语部分. * |
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