CN101063163A - Method for detecting foodstuff packaging material surface fungus and special filter paper - Google Patents
Method for detecting foodstuff packaging material surface fungus and special filter paper Download PDFInfo
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- CN101063163A CN101063163A CN 200710014458 CN200710014458A CN101063163A CN 101063163 A CN101063163 A CN 101063163A CN 200710014458 CN200710014458 CN 200710014458 CN 200710014458 A CN200710014458 A CN 200710014458A CN 101063163 A CN101063163 A CN 101063163A
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Abstract
The invention discloses a detecting method of food packaging material inner surface fungus, which comprises the following steps: comprising private medium and production of medium filter paper; daubing the filter paper in setting area of program food packaging bag; or daubing cleaning mixture of packing vessel on the filter paper; putting the filter paper into sterile culture dish; culturing at 28-30 deg. c; watching and counting colony count on the surface of filter paper; counting fungus number in unit area; judging the fungus. This method can save time of medium allocation and decrease labor strength.
Description
Technical field
The present invention relates to a kind of microorganism detection method, relate in particular to a kind of detection method and special filter paper of foodstuff packaging material surface fungus, belong to the food hygiene detection technical field.
Background technology
Mould for foodstuff packaging material surface detects still not unified standard at present, still colony counting methods that adopt classics are about to be dissolved in the sterilized water of certain volume dilution back spread plate after a certain amount of sample to be checked is pulverized more, by cultivating the colony number on the statistics flat board.And at present packaging material for food mould examination criteria is only required its inner aseptic getting final product, and for the appearance no requirement (NR), its judgement criteria is the microorganism number that contains of unit surface normally.Therefore, the classic flat-plate method has certain limitation for the detection of packaging material for food, and procedure is loaded down with trivial details, and traditional method also needs in earlier stage numerous and diverse preparation works such as the sterilising treatment of substratum and preparation and cultivation vessel, therefore, it is very necessary and urgent to set up a kind of method for quick at contained fungi count in the packaging material for food unit surface.
Summary of the invention
At deficiencies such as big surface-area of packaging material for food and classic flat-plate detection technique complex operations, the problem to be solved in the present invention provides a kind of detection method and special filter paper of foodstuff packaging material surface fungus, to simplify and to accelerate the detection method of mould, improve detection efficiency to foodstuff packaging material surface fungus.
The detection method of foodstuff packaging material surface fungus of the present invention comprises the steps:
(1) special filter paper preparation: filter paper is cut into the filter paper that area is 6～10cm * 6～10cm, 115 ℃ of sterilization 20min, above-mentioned filter paper is soaked into to drenched with sterilized mould medium, vacuum-drying or change in 80 ℃ of loft drier then, dry and take out filter paper after 2～3 hours, put into the culture dish of sterilization, standby;
(2) with the wetting filter paper of sterilized water elder generation, then this filter paper is set on food pack to be measured surface and repeated erasing 3～4 times in the area, again this filter paper is put into sterile petri dish, place 28～30 ℃ to cultivate 36～48 hours down, each sample repeats to do 3～5, observe and count the colony number that the filter paper surface mould forms, the fungi count that the Units of Account area contains judges with this whether foodstuff packaging material surface fungus detects qualified;
Or (2) are poured quantitative sterilized water earlier in the food container to be detected, shake, with the water washing container inner wall, getting washings afterwards evenly is added drop-wise on the aseptic filter paper sheet, place 28～30 ℃ to cultivate 36～48 hours down, each sample repeats to do 3～5, observes and count the colony number that the filter paper surface mould forms, the fungi count that the Units of Account area contains judges with this whether foodstuff packaging material surface fungus detects qualified;
Wherein: the described mould medium prescription of step (1) is: yeast powder 1.5 grams, Tryptones 0.5 gram, glucose 5 grams, potassium primary phosphate 0.3 gram, sal epsom 0.15 gram, 1/3000 rose-bengal solution 2mL, distilled water 100mL, pH nature.
In the detection method of above-mentioned foodstuff packaging material surface fungus: the described filter paper area of step (1) is preferably 6cm * 6cm.
In the detection method of above-mentioned foodstuff packaging material surface fungus: preferably 30 ℃ of the described culture temperature of step (2), described incubation time preferably 40 hours.
Foodstuff packaging material surface fungus of the present invention detects special filter paper, it is characterized in that: described special filter paper is made the filter paper of sterilization by the mould medium of sterilization after the fully wetting and oven dry, wherein: described mould medium prescription is: yeast soaks powder 1.5 grams, Tryptones 0.5 gram, glucose 5 grams, potassium primary phosphate 0.3 gram, sal epsom 0.15 gram, 1/3000 rose-bengal solution 2mL, distilled water 100ml, pH nature.
The sterilising conditions of above-mentioned steps (1) or described filter paper of step (2) or mould medium is: sterilized 20 minutes for 115 ℃.The filter paper that described filter paper is selected for use and breathed freely, adsorptivity is strong.
The present invention obtains a kind of substratum that is suitable for common mould-growth by medium optimization; As culture medium carrier,, be prepared into the filter paper that contains the abundant nutrition composition with good permeability, strong, the suitable big or small filter paper material of adsorptivity through filter paper abundant wetting and processed in mould medium; According to detecting packaging material for food, adopt erasing or washings to coat mode such as filter paper and realize that packaging material for food is carried out quick mould to be detected.
Foodstuff packaging material surface fungus of the present invention detects that special filter paper has can long-term storage, and the characteristics of applying flexible can have been saved the time that substratum preparation, required sterilizing containers, cleaning etc. are handled greatly, have reduced labour intensity and have saved detection time.
Embodiment
Embodiment 1
Take by weighing peptone, yeast powder, glucose is dissolved in the 500mL distilled water with formula rate, and the pH nature with the rose-bengal solution of formula rate adding 1/3000, is put into high-pressure steam sterilizing pan with the substratum of preparation, sterilizes 20 minutes for 115 ℃.
Filter paper is cut into 6cm * 6cm size, packages, put into high-pressure steam sterilizing pan, sterilized 20 minutes for 115 ℃ with kraft paper.
The filter paper of sterilization is soaked into to drenched with sterilized substratum, put into 80 ℃ of loft drier oven dry 2 hours then, take out, put into the sterile petri dish that diameter is 9cm, cryopreservation, stand-by.
Soak into filter paper with sterilized water 2mL, then this filter paper is repeated to smear 3 times in the certain area of food pack to be measured, again this filter paper is put into sterile petri dish, place 30 ℃ to cultivate 48 hours down, each sample is done 3 repetitions, observe and count the colony number that the filter paper surface mould forms, the fungi count that the Units of Account area contains judges with this whether foodstuff packaging material surface fungus detects qualified.
Embodiment 2
Take by weighing peptone, yeast powder, glucose is dissolved in the 500mL distilled water with formula rate, and the pH nature with the rose-bengal solution of formula rate adding 1/3000, is put into high-pressure steam sterilizing pan with the substratum of preparation, sterilizes 20 minutes for 115 ℃.
Filter paper is cut into 8cm * 8cm size, packages, put into high-pressure steam sterilizing pan, sterilized 20 minutes for 115 ℃ with kraft paper.
The filter paper of sterilization is soaked into to drenched with sterilized substratum, put into 80 ℃ of loft drier oven dry 2 hours then, take out, put into the sterile petri dish that diameter is 12cm, cryopreservation, stand-by.
Get the sterilized water of set amount, put into food product packets tinning to be measured (bottle), washing tank (bottle) body, get the 3mL washings, evenly be added drop-wise on the aseptic filter paper sheet of above-mentioned 8cm * 8cm, place 28 ℃ to cultivate 45 hours down, each sample is done 3 repetitions, observe and count the colony number that the filter paper surface mould forms, the fungi count that the Units of Account area contains judges with this whether foodstuff packaging material surface fungus detects qualified.
Embodiment 3
(1) special filter paper preparation: filter paper is cut into the filter paper that area is 10cm * 10cm, sterilization.The filter paper of sterilization is soaked into to drenched with sterilized mould medium, and vacuum-drying is then taken out filter paper and put into sterile petri dish, and is standby;
(2) with the wetting filter paper of sterilized water elder generation, then this filter paper is set on food product pack to be measured surface and repeated erasing 4 times in the area, again this filter paper is put into sterile petri dish, place 29 ℃ to cultivate 38 hours down, each sample repeats to do 5, observe and count the colony number that the filter paper surface mould forms, the fungi count that the Units of Account area contains judges with this whether foodstuff packaging material surface fungus detects qualified;
Wherein: the described mould medium prescription of step (1) is: yeast soaks powder 1.5 grams, Tryptones 0.5 gram, glucose 5 grams, potassium primary phosphate 0.3 gram, sal epsom 0.15 gram, 1/3000 rose-bengal solution 2mL, distilled water 100ml, pH nature.
The sterilising conditions of above-mentioned steps (1) or described filter paper of step (2) or mould medium is: sterilized 20 minutes for 115 ℃.
Embodiment 4
(1) special filter paper preparation: filter paper is cut into the filter paper that area is 7cm * 7cm, sterilization.The filter paper of sterilization is soaked into to drenched with sterilized mould medium, change over to then in 80 ℃ of loft drier, dry and take out filter paper after 3 hours, put into sterile petri dish, standby;
(2) quantitative sterilized water is poured into earlier in the food container to be detected, shake, with the water washing container inner wall, getting washings afterwards evenly is added drop-wise on the aseptic filter paper sheet, place 30 ℃ to cultivate 48 hours down, each sample repeats to do 3, observes and count the colony number that the filter paper surface mould forms, the fungi count that the Units of Account area contains judges with this whether foodstuff packaging material surface fungus detects qualified;
Wherein: the described mould medium prescription of step (1) is: yeast soaks powder 1.5 grams, Tryptones 0.5 gram, glucose 5 grams, potassium primary phosphate 0.3 gram, sal epsom 0.15 gram, 1/3000 rose-bengal solution 2mL, distilled water 100ml, pH nature.
The sterilising conditions of above-mentioned steps (1) or described filter paper of step (2) or mould medium is: sterilized 20 minutes for 115 ℃.
Claims (4)
1. the detection method of a foodstuff packaging material surface fungus comprises the steps:
(1) special filter paper preparation: filter paper is cut into the filter paper that area is 6～10cm * 6～10cm, 115 ℃ of sterilization 20min; Above-mentioned filter paper is soaked into to drenched with sterilized mould medium, and vacuum-drying or change in 80 ℃ of loft drier is then dried and taken out filter paper after 2～3 hours, puts into sterile petri dish, and is standby;
(2) with the wetting filter paper of sterilized water elder generation, then this filter paper is set on food pack to be measured surface and repeated erasing 3～4 times in the area, again this filter paper is put into sterile petri dish, place 28～30 ℃ to cultivate 36～48 hours down, each sample repeats to do 3～5, observe and count the colony number that the filter paper surface mould forms, the fungi count that the Units of Account area contains judges with this whether foodstuff packaging material surface fungus detects qualified;
Or (2) are poured quantitative sterilized water earlier in the food container to be detected, shake, with the water washing container inner wall, getting washings afterwards evenly is added drop-wise on the aseptic filter paper sheet, place 28～30 ℃ to cultivate 36～48 hours down, each sample repeats to do 3～5, observes and count the colony number that the filter paper surface mould forms, the fungi count that the Units of Account area contains judges with this whether foodstuff packaging material surface fungus detects qualified;
Wherein: the described mould medium prescription of step (1) is: yeast soaks powder 1.5 grams, Tryptones 0.5 gram, glucose 5 grams, potassium primary phosphate 0.3 gram, sal epsom 0.15 gram, 1/3000 rose-bengal solution 2mL, distilled water 100ml, pH nature.
2. the detection method of foodstuff packaging material surface fungus according to claim 1, it is characterized in that: the described filter paper area of step (1) is 6cm * 6cm.
3. the detection method of foodstuff packaging material surface fungus according to claim 1, it is characterized in that: the described culture temperature of step (2) is 30 ℃, and described incubation time is 40 hours.
4. foodstuff packaging material surface fungus detects special filter paper, it is characterized in that: described special filter paper is made the filter paper of sterilization by the mould medium of sterilization after the fully wetting and oven dry, wherein: described mould medium prescription is: yeast soaks powder 1.5 grams, Tryptones 0.5 gram, glucose 5 grams, potassium primary phosphate 0.3 gram, sal epsom 0.15 gram, 1/3000 rose-bengal solution 2mL, distilled water 100ml, pH nature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200710014458 CN101063163A (en) | 2007-05-23 | 2007-05-23 | Method for detecting foodstuff packaging material surface fungus and special filter paper |
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| CN 200710014458 CN101063163A (en) | 2007-05-23 | 2007-05-23 | Method for detecting foodstuff packaging material surface fungus and special filter paper |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102151437A (en) * | 2011-01-28 | 2011-08-17 | 厦门建霖工业有限公司 | PP (polypropylene) filter paper with functions of resisting bacteria, dechlorinating and removing heavy metal ions and preparation method thereof |
| CN102337324A (en) * | 2011-10-24 | 2012-02-01 | 西南大学 | Preparation method of adsorption pad type culture medium for detecting microorganisms |
| CN112322692A (en) * | 2020-11-09 | 2021-02-05 | 安徽屾远材料技术有限公司 | Mould rapid detection patch and preparation method and use method thereof |
-
2007
- 2007-05-23 CN CN 200710014458 patent/CN101063163A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102151437A (en) * | 2011-01-28 | 2011-08-17 | 厦门建霖工业有限公司 | PP (polypropylene) filter paper with functions of resisting bacteria, dechlorinating and removing heavy metal ions and preparation method thereof |
| CN102337324A (en) * | 2011-10-24 | 2012-02-01 | 西南大学 | Preparation method of adsorption pad type culture medium for detecting microorganisms |
| CN112322692A (en) * | 2020-11-09 | 2021-02-05 | 安徽屾远材料技术有限公司 | Mould rapid detection patch and preparation method and use method thereof |
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Open date: 20071031 |