CN101059501B - Preparation method of collection plate for assay - Google Patents
Preparation method of collection plate for assay Download PDFInfo
- Publication number
- CN101059501B CN101059501B CN2007100223245A CN200710022324A CN101059501B CN 101059501 B CN101059501 B CN 101059501B CN 2007100223245 A CN2007100223245 A CN 2007100223245A CN 200710022324 A CN200710022324 A CN 200710022324A CN 101059501 B CN101059501 B CN 101059501B
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- China
- Prior art keywords
- carrier
- slide surface
- adhesive
- microslide
- film
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Abstract
The invention relates to a method for preparing analysis liquid collecting plate of carrier, comprising that washing and drying the surface of carrier, coating light sensitive adhesive on idle part, solidifying via ultraviolet light, putting a clamper with the carrier into a vacuum room, vacuuming to 10<-3>Pa, adding argon gas, making vacuum degree to 0-10Pa, adding voltage on target material, generating glow discharge between the target material and the clamper to form a plasma area, processing magnetic-control splash, depositing film on the surface of the carrier, removing the light-sensitiveadhesive, coating connecting adhesive on the adhesive part of a cover, adhering the cover to the plated surface of the carrier, heating and drying the adhesive. The invention can resolve the defects of low accuracy and mistake caused by laminated cell layer. The invention uses capillary action on the surface of carrier, to expand cell group via analysis liquid along the container formed by film, to be detected under microscope, to improve accuracy.
Description
Technical field
The present invention relates to a kind of chemical examination liquid harvester, particularly a kind of preparation method of the chemical examination liquid collection plate to microslide.
Background technology
Before the present invention, existing chemical examination collection to microslide exists following defective.The overlapping analysis result poor accuracy that causes of cellular layer, or even erroneous judgement.This method be with the analysis drop in slide surface, firmly get rid of flat liquid then, or liquid pushed away flat with another sheet microslide, measure again, this causes measuring accuracy to reduce greatly certainly, and analysis result is inaccurate naturally, even feasible measurement can't be carried out at all.
Summary of the invention
Purpose of the present invention just is to overcome above-mentioned defective, develops a kind of preparation method of novel collection plate for assay.
Technical scheme of the present invention is:
The preparation method of collection plate for assay, its major technique step is
(1) cleans, dries up slide surface;
(2) in slide surface blank parts coating photoresists, ultraviolet curing;
(3) anchor clamps that microslide will be housed are put into vacuum chamber, are evacuated to 10
-3Pa;
(4) charge into argon gas in vacuum chamber, vacuum tightness reaches 0～10Pa;
(5) apply voltage to target, between target and anchor clamps, produce glow discharge, form plasma slab;
(6) carry out magnetron sputtering, at the slide surface deposit film;
(7) photoresists of removal slide surface;
(8) evenly apply the connection glue in cover glass adhesion coating area part;
(9) cover glass is adhered to the coated surface of microslide, and the heating, drying glue.
Advantage of the present invention and effect are to adopt and are coated with definite shape and certain thickness, uniform film in slide surface, utilization capillary action principle, analytic liquid evenly launches cell mass along the container area that film forms between cover glass lower surface and microslide upper surface, form one deck analysis layer, very convenient in examining under a microscope, and improved the measurement statistical accuracy greatly.
This method has that coating is even, bright and clean, difficult drop-off; Analytic liquid flattens advantages such as speed is fast, processing is clean, easy to operate, analysis is accurate.Can be widely used in the data statistics of health care, vegeto-animal analysis and various precision measurements.
Description of drawings
Fig. 1---chemical examination liquid collection plate planimetric map.
Lid fragmentation adhesion district 1, lid fragmentation adhesion coating area 2, chemical examination liquid flattened region 3, chemical examination liquid buffer zone 4, chemical examination liquid inlet 5.
Fig. 2---cover glass structure knot shape synoptic diagram.
Fig. 3---the plated film synoptic diagram of chemical examination liquid collection plate.
Electric rotating machine 6, target 7, work rest 8, thermopair 9, Ar inflation inlet 10, vacuum pumping opening 11, measured film thickness instrument 12, vacuum chamber 13.
Embodiment
Collection plate is to be formed by the glue adhesion by microslide of surface coating (Fig. 1) and cover glass (Fig. 2), in lid fragmentation adhesion district 1, lid fragmentation adhesion coating area 2 is coated glue; At the top is inlet 5 positions of chemical examination liquid.Wherein, after chemical examination liquid injects from inlet 5, between the lower surface of the upper surface of microslide and cover glass, flatten along coating film area, in chemical examination liquid flattened region 3, fully launch by capillary action, form a unimolecule cell analysis layer, the analytic liquid of redundance will enter chemical examination liquid buffer zone 4, thereby guaranteed that most important analysis area part can form uniform monolayer in the collection plate, the very few statistics that causes of the excessive and liquid of effectively having avoided liquid too much to cause phenomenon on the low side is placed on microscopically with collection plate after liquid to be analyzed flattens fully and carries out observation analysis.
At first slide surface is cleaned:
With alcohol and ether mixed liquor, press the 3:1 proportioning and soak, remove greasy dirt; Use ultrasonic cleaning again; Dry with acetate at last and dry up slide surface with dry heat blower fan and flat spout.
Carry out making sheet:
According to shown in Figure 1, evenly apply photoresists, ultraviolet curing in the slide surface blank parts.
Magnetron sputtering:
As shown in Figure 3, be placed on microslide shown in Figure 1 on the work rest 8 and put into vacuum chamber 13, start electric rotating machine 6, make wave carrier piece with work rest 8 rotations; By vacuum pumping opening 11 vacuum chamber 13 is evacuated to 10
-3Pa; Charge into argon gas by Ar inflation inlet 10 in vacuum chamber 13, vacuum tightness reaches 0～10Pa, and this example is 2Pa, applies voltage to target 7, produces glow discharge and forms plasma; By the surface temperature in the thermopair 9 MEASUREMENTS OF THIN deposition process; Determine the thickness of film according to the cell dia of measuring samples, and pass through FTM-III (C) quartz crystal measured film thickness instrument 12 on-line monitoring growth for Thin Film thickness; Film thickness equals to measure the maximum gauge of cell, at 6～9um, is set at 9.0um as the red blood cell diameter scope, neutrophil leucocyte, and diameter 10～12 μ m are set at 12.0um; On measured film thickness instrument 12, set thin film deposition thickness; Regulate sputtering power,, arrive and cut off shielding power supply when setting thickness automatically at the slide surface deposit film; Remove the photoresists of slide surface, stay figure shown in Figure 1; Lid fragmentation adhesion coating area 2 parts in Fig. 1 evenly apply the connection glue; Adopt cover glass shown in Figure 2, adhere to the lid fragmentation adhesion district 1 of the coated surface of microslide among Fig. 1, and the heating, drying glue, container type chemical examination liquid collection plate formed.
Claims (1)
1. the preparation method of collection plate for assay, its step is
(1) cleans, dries up slide surface;
(2) in slide surface blank parts coating photoresists, ultraviolet curing;
(3) anchor clamps that microslide will be housed are put into vacuum chamber, are evacuated to 10
-3Pa;
(4) charge into argon gas in vacuum chamber, vacuum tightness reaches 0～10Pa;
(5) apply voltage to target, between target and anchor clamps, produce glow discharge, form plasma slab;
(6) carry out magnetron sputtering, at the slide surface deposit film, film thickness equals to measure the maximum gauge of cell;
(7) photoresists of removal slide surface;
(8) evenly apply the connection glue in cover glass adhesion coating area part;
(9) cover glass is adhered to the coated surface of microslide, and the heating, drying glue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100223245A CN101059501B (en) | 2007-05-14 | 2007-05-14 | Preparation method of collection plate for assay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100223245A CN101059501B (en) | 2007-05-14 | 2007-05-14 | Preparation method of collection plate for assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101059501A CN101059501A (en) | 2007-10-24 |
| CN101059501B true CN101059501B (en) | 2011-08-17 |
Family
ID=38865721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100223245A Expired - Fee Related CN101059501B (en) | 2007-05-14 | 2007-05-14 | Preparation method of collection plate for assay |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101059501B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105628476B (en) * | 2016-03-21 | 2018-12-21 | 厦门通灵生物医药科技有限公司 | A kind of multichannel treating device for pathology specimen |
| CN112577794A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Method for preparing film-taking enriched bacteria by shadowless adhesive and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1195113A (en) * | 1998-04-17 | 1998-10-07 | 徐祝平 | Plastic slide and making method thereof |
| CN1335501A (en) * | 2001-04-27 | 2002-02-13 | 上海晶泰生物技术有限公司 | Multiple-sample microarray biochip |
| US20060121197A1 (en) * | 2003-09-05 | 2006-06-08 | Moffat William A | Method for silane coating of indium tin oxide surfaced substrates |
| CN1935724A (en) * | 2005-09-20 | 2007-03-28 | 徐志国 | Process for manufacturing positive charge colour glass slide |
-
2007
- 2007-05-14 CN CN2007100223245A patent/CN101059501B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1195113A (en) * | 1998-04-17 | 1998-10-07 | 徐祝平 | Plastic slide and making method thereof |
| CN1335501A (en) * | 2001-04-27 | 2002-02-13 | 上海晶泰生物技术有限公司 | Multiple-sample microarray biochip |
| US20060121197A1 (en) * | 2003-09-05 | 2006-06-08 | Moffat William A | Method for silane coating of indium tin oxide surfaced substrates |
| CN1935724A (en) * | 2005-09-20 | 2007-03-28 | 徐志国 | Process for manufacturing positive charge colour glass slide |
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| Publication number | Publication date |
|---|---|
| CN101059501A (en) | 2007-10-24 |
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Granted publication date: 20110817 Termination date: 20140514 |