CN101059438A - High flux real-time minimum multifunctional fluorescent detector - Google Patents
High flux real-time minimum multifunctional fluorescent detector Download PDFInfo
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- CN101059438A CN101059438A CN 200710022844 CN200710022844A CN101059438A CN 101059438 A CN101059438 A CN 101059438A CN 200710022844 CN200710022844 CN 200710022844 CN 200710022844 A CN200710022844 A CN 200710022844A CN 101059438 A CN101059438 A CN 101059438A
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Abstract
The invention discloses a high-flux real-time micro multifunctional fluorescence detector, comprising a wideband light source (1), a beam reducing lens, a digit micro lens array (2), a sample board, a radiating object lens (8), a collecting object lens (7), a beam splitter (5), and a spectrometer (6). The invention is characterized in that the sample board is a sample porous board (3) arranged with a plurality of holes which bottom is connected with a capillary (4), the capillary (4) comprises a sample which mark material is nanometer fluorescence material or quantum point, and the sample is processed in the capillary (4). The invention has high flux which can real-time detect multiply samples.
Description
Technical field
The present invention relates to a kind of high flux real-time minimum multifunctional fluorescent detector, it is applicable to a plurality of fields such as food, medicine detection, environment measuring drug screening, molecular biology, immunology, cytology, biological chemistry, microbial identification.
Background technology
What at present traditional detection system adopted is the method that simple sample is measured one by one, can not realize that up to a hundred samples measure and write down the function of all band spectrum simultaneously.And the whole world all is traditional measurement methods of narrow wave band or single wavelength at present, full wave measurement also can not break through technically, the quantity of information that is obtained can't satisfy the needs of scientific experiment, also can't reflect the form of biomolecule and cell and the real-time change of structure.And experimental technique now is very consuming time, and cost is also high.The present cell sample that is detected is for fixedly to be placed on the sample panel, and is mobile relatively poor, sensitivity and less stable when detecting.
Summary of the invention
The invention provides a kind of high flux real-time minimum multifunctional fluorescence detecting system, it is the flux height not only, and can realize that a plurality of samples measure in real time.
The present invention has adopted following technical scheme: a kind of high flux real-time minimum multifunctional fluorescent detector, it comprises that wide frequency light source, light beam subtract shape mirror, digital micromirror array, sample panel, illumination objective, collection object lens, spectroscope, spectrogram instrument, described sample panel is set to the sample microwell plate, on the sample microwell plate, be provided with some holes, bottom in each hole is connected with kapillary, the sample of nano fluorescent material or quantum dot is housed in kapillary, and sample detects in kapillary and finishes.
Described capillary diameter is the 0.1-1 micron.Described wide frequency light source is set to ozone-free Xe lamp, and ozone-free Xe lamp is fixed on the base of adjusting.Be provided with the polymers mirror in the described wide frequency light source, the non-disc lens combination that the polymers mirror is made for founding silicon dioxide, lens combination is installed in the box of light source, and the polymers mirror is arranged in the light source box relative with light source.The delivery outlet of described light source is provided with groove.Described spectrogram instrument is provided with the CCD camera.Described spectrogram instrument is connected with computing machine.Described sample microwell plate is 96/384/1536 hole.
The present invention has following beneficial effect: the present invention is by adopting biological microfluidic technology, be provided with kapillary by bottom at sample 96/384/1536 microwell plate, the sample cell that detects is placed in the kapillary, sample cell can be in flow in capillary tube, do not need optical filtering can so that exciting light from fluorescence emission, rejected completely, significantly reduce the cost of system, and can gather the full wave continuous absorption spectrum and the fluorescence spectrum of 96-384 sample simultaneously, form spectrogram by the spectrogram instrument, improved detection efficiency, can also all detect in real time at each crucial moment, can provide valuable information for research.Sample spectra is measured and can be carried out simultaneously and have no the mistiming in addition.Therefore measure can shortening in 1 second T.T. of all samples in 96/384/1536 microwell plate.The present invention utilizes non-disc lens combination focal spot circular in the light source to be converted into the focused beam of bar shaped.Can reduce the focusing deviation that aberration causes like this.Light beam subtracts the shape mirror can reach the scope that focus lamp and microcobjective are accepted with 5 times of the reduced of directional light.Light source of the present utility model adopts ozone-free Xe lamp, and it can realize the luminous flux more than 60%.What fluorescer of the present invention adopted is nano fluorescent or quantum dot, can improve the sensitivity when detecting like this, and measuring stability is strengthened.
Description of drawings
Fig. 1 is a structural representation of the present invention
Fig. 2 is the index path of light source of the present invention
Embodiment
In Fig. 1 and Fig. 2, a kind of high flux real-time minimum multifunctional fluorescent detector, it comprises wide frequency light source 1, light beam subtracts the shape mirror, DLP numeral micromirror array 2, sample panel, illumination objective 8, collect object lens 7, spectroscope 5, spectrogram instrument 6, wide frequency light source 1 is set to ozone-free Xe lamp, ozone-free Xe lamp is fixed on the base of adjusting, in wide frequency light source, be provided with polymers mirror (10), the non-disc lens combination that polymers mirror (10) is made for founding silicon dioxide, lens combination is installed in the box of light source, polymers mirror (10) (10) is arranged in the light source box relative with light source, the delivery outlet of light source is provided with the groove sample panel and is set to sample microwell plate 3, sample microwell plate 3 is 96/384/1536 hole, be connected with the kapillary that length is the 0.1-1 micron in the bottom in each hole, be placed with the sample that label is nano fluorescent or quantum in the kapillary, sample detects in kapillary 4 and finishes.Spectrogram instrument 6 is provided with CCD camera 9, and the spectrogram instrument is connected with computing machine.
Wide frequency light source: it can be used for shining the sample that has fluorescer, is used for excitation nano fluorescence or quantum emission light;
Polymers mirror (10): polymers mirror (10) is arranged in the light source box relative with light source, and wide frequency light source shines on the polymers mirror (10), and the light that polymers mirror (10) collection light source sends makes its parallel emission,
Light beam subtracts the shape mirror: the directional light of light source by polymers mirror (10) reduces through the diameter that light beam subtracts the directional light that the shape mirror comes out condenser,
Numeral micromirror array and illumination objective: subtract that directional light that the shape mirror comes out forms independent optical fiber through digital micromirror array and by the output of illumination objective direct irradiation by light beam;
Sample 96/384/1536 microwell plate, be used to place test sample, bottom at sample 96/384/1536 microwell plate is provided with kapillary, sample detects in kapillary, the nano fluorescent or the quantum emission light that are radiated at sample through the zero diopter of digital micromirror array and illumination objective can be in a plurality of light beams of different wavelengths such as 200-1000 nanometers
Spectroscope is used for the light beam of some wavelength is rejected, some other light beam process fluorescence microscopy object lens continuous spectrum,
The spectrogram instrument is noted continuous spectrum formation continuous spectrum image and is passed through the output of CCD camera imaging.Spectrogram is connected with computing machine, and the computing machine inherence is equipped with the SoftDT analysis software and is carried out spectral analysis.
Collect object lens, collect some light beam irradiates of spectroscope rejecting and to the sample of 96/384/1536 microwell plate, proceed cycle detection.
Claims (8)
1, a kind of high flux real-time minimum multifunctional fluorescent detector, it comprises that wide frequency light source (1), light beam subtract shape mirror, digital micromirror array (2), sample panel, illumination objective (8), collect object lens (7), spectroscope (5), spectrogram instrument (6), it is characterized in that described sample panel is set to sample microwell plate (3), on sample microwell plate (3), be provided with some holes, be connected with kapillary (4) in the bottom in each hole, the sample that label is nano fluorescent material or quantum dot is housed in kapillary (4), and sample detects in kapillary (4) and finishes.
2, high flux real-time minimum multifunctional fluorescent detector according to claim 1 is characterized in that described kapillary (4) diameter is the 0.1-1 micron.
3, high flux real-time minimum multifunctional fluorescent detector according to claim 1 is characterized in that described wide frequency light source is set to ozone-free Xe lamp, and ozone-free Xe lamp is fixed on the base of adjusting.
4, high flux real-time minimum multifunctional fluorescent detector according to claim 1, it is characterized in that being provided with in the described wide frequency light source (1) polymers mirror (10), the non-disc lens combination that polymers mirror (10) is made for founding silicon dioxide, lens combination is installed in the box of light source, and polymers mirror (10) is arranged in the light source box relative with light source.
5, high flux real-time minimum multifunctional fluorescence detecting system according to claim 1 is characterized in that the delivery outlet of described light source is provided with groove.
6, high flux real-time minimum multifunctional fluorescence detecting system according to claim 1 is characterized in that described spectrogram instrument (6) is provided with CCD camera (9).
7, high flux real-time minimum multifunctional fluorescence detecting system according to claim 1 is characterized in that described spectrogram instrument is connected with computing machine.
8, high flux real-time minimum multifunctional fluorescence detecting system according to claim 1 is characterized in that described sample microwell plate (3) is 96/384/1536 hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100228446A CN100543460C (en) | 2007-05-24 | 2007-05-24 | A kind of high flux real-time minimum multifunctional fluorescent detector |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2007100228446A CN100543460C (en) | 2007-05-24 | 2007-05-24 | A kind of high flux real-time minimum multifunctional fluorescent detector |
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| Publication Number | Publication Date |
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| CN101059438A true CN101059438A (en) | 2007-10-24 |
| CN100543460C CN100543460C (en) | 2009-09-23 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514987B (en) * | 2008-07-14 | 2012-11-21 | 马义才 | System for quantitative detection of quanta dot mark test bar and detection method thereof |
| CN103782155A (en) * | 2011-09-06 | 2014-05-07 | 皇家飞利浦有限公司 | Optical biosensor with a plurality of sensors regions |
| CN105717086A (en) * | 2016-03-07 | 2016-06-29 | 吉林大学 | Frequency conversion control method for light source of atomic fluorescence spectrometer based on digital micro-mirror |
| CN109813692A (en) * | 2019-01-02 | 2019-05-28 | 北京科技大学 | A kind of capillary analysis detection method based on ultrasound aggregation |
| CN112782131A (en) * | 2019-11-11 | 2021-05-11 | 成都辰显光电有限公司 | Spectrum detection system and spectrum detection method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5517313A (en) * | 1995-02-21 | 1996-05-14 | Colvin, Jr.; Arthur E. | Fluorescent optical sensor |
| US6654119B1 (en) * | 1999-04-21 | 2003-11-25 | Chromagen, Inc. | Scanning spectrophotometer for high throughput fluroescence detection |
| US6323495B1 (en) * | 1999-09-24 | 2001-11-27 | Umm Electronics, Inc. | Method and apparatus for the determination of phase delay in a lifetime fluorometer without the use of lifetime standards |
| FR2806159B1 (en) * | 2000-03-09 | 2003-03-07 | Lorraine Inst Nat Polytech | OPTICAL METHOD AND DEVICE FOR NON-INTRUSIVE TEMPERATURE MEASUREMENT IN A FLOWING LIQUID |
| CN1235038C (en) * | 2003-05-28 | 2006-01-04 | 中国科学院大连化学物理研究所 | Capillary array electrophoretic ratating laser scanning co-focusing fluorescent inspecting instrument |
| US7031084B2 (en) * | 2003-07-23 | 2006-04-18 | Eastman Kodak Company | Imaging system using combined dichroic/high-pass filters |
-
2007
- 2007-05-24 CN CNB2007100228446A patent/CN100543460C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514987B (en) * | 2008-07-14 | 2012-11-21 | 马义才 | System for quantitative detection of quanta dot mark test bar and detection method thereof |
| CN103782155A (en) * | 2011-09-06 | 2014-05-07 | 皇家飞利浦有限公司 | Optical biosensor with a plurality of sensors regions |
| CN103782155B (en) * | 2011-09-06 | 2016-11-16 | 皇家飞利浦有限公司 | There is the optical biosensor of multiple sensor region |
| CN105717086A (en) * | 2016-03-07 | 2016-06-29 | 吉林大学 | Frequency conversion control method for light source of atomic fluorescence spectrometer based on digital micro-mirror |
| CN105717086B (en) * | 2016-03-07 | 2018-03-27 | 吉林大学 | AFS light source method for controlling frequency conversion based on digital micro-mirror |
| CN109813692A (en) * | 2019-01-02 | 2019-05-28 | 北京科技大学 | A kind of capillary analysis detection method based on ultrasound aggregation |
| CN112782131A (en) * | 2019-11-11 | 2021-05-11 | 成都辰显光电有限公司 | Spectrum detection system and spectrum detection method |
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| Publication number | Publication date |
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| CN100543460C (en) | 2009-09-23 |
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