CN101058808B - Breast cancer relevant p60 gene, coding protein and application thereof - Google Patents
Breast cancer relevant p60 gene, coding protein and application thereof Download PDFInfo
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- CN101058808B CN101058808B CN2006100762782A CN200610076278A CN101058808B CN 101058808 B CN101058808 B CN 101058808B CN 2006100762782 A CN2006100762782 A CN 2006100762782A CN 200610076278 A CN200610076278 A CN 200610076278A CN 101058808 B CN101058808 B CN 101058808B
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Abstract
The invention discloses a p60 gene and its coded Q9H972-2 protein, which also provides an application of the gene and protein in the medicine, wherein the protein participates the occurrence and development of breast cancer, which provides cue and method for theoretical study and clinical therapy of breast cancer.
Description
Technical field
The present invention relates to a kind of and breast cancer related p60 gene, the Q9H9 72-2 albumen of this genes encoding, and described gene and the application of albumen in medical science.Described albumen participates in developing of mammary cancer, for the theoretical investigation of mammary cancer and clinical treatment is given a clue and thinking.
Background technology
Some albumen can suppress or promote the growth and the propagation of tumour cell, finds new inhibition or promotes tumor growth and the gene of propagation and encoded protein can be tumor treatment new ways and means is provided.
Summary of the invention
The present invention has found a kind of PQ 9H972-2 that participates in mammary cancer generation evolution, cloned this proteic gene p60 of coding, made up the host cell that contains this expression carrier and contain this expression vector, and find that this albumen can influence the growth and the propagation of tumour cells such as mammary cancer, based on above discovery, finished the present invention.
P60 has another name called HGNC:20162, FLJ12154.Be positioned karyomit(e) 14q11.2, cDNA total length 2352bp, the about 60kD of expressing protein, ORF is long to be 1617bp, 539 amino acid of encoding.
The homologous sequence comparison
Kind sequence number feature
Pongo?pygmaeus CR85760.1 cDNA?DKFZp468C2110
Canis familiaris NM_537369.2 is similar to PROTEIN C 14 or f93 precursor
Bos taurus NM_583415.2 is similar to PROTEIN C 14 or f93 precursor
Pan troglodytes XM_522798.1 is similar to PROTEIN C 14 or f93 precursor
Rattus XM_573773.1 is similar to RIKEN cDNA 4931414P19
norvegicus
The present invention in the MCF-7 cell, expresses the p60 gene pcDNA3.1 (-)-p60 expression plasmid transfection in this cell, extract total protein of cell, and Western Blot has identified the existence of p60 gene expression product.
Mtt assay detects the influence of p60 gene expression product to breast cancer cell propagation, and the result shows that the p60 gene expression product plays restraining effect to the propagation of breast cancer cell.
Therefore, according to the present invention, provide:
1, can influence the growth of tumour cell and the albumen of propagation, especially recombinant protein, this albumen has the aminoacid sequence shown in the sequence 2 in the sequence table, perhaps by the one or more amino acid whose disappearance in the described aminoacid sequence, replaces or increases and the aminoacid sequence that obtains.
2, the above-mentioned proteic gene of coding, it be have the DNA of the base sequence shown in the sequence 1 or its complementary sequence or with the DNA of described DNA hybridize under stringent condition.
3, contain the said gene or the above-mentioned proteic expression carrier of encoding.
4, by above-mentioned expression vector transformed host cells.
5, produce the method for recombinant protein of the present invention, this method comprises cultivates above-mentioned transformed host cells, makes transformant produce recombinant protein of the present invention.
6, at as above 1 described proteic antibody, the specific antibody that it is to use described recombinant protein to produce as immunogen.It can be mono-clonal or polyclonal antibody.
7, above 1 described albumen or above the application of 2 described genes in the medicine of preparation treatment mammary cancer.
8, the pharmaceutical composition that contains above 1 described albumen and pharmaceutically acceptable carrier.
9, a kind ofly be used for the primer that carries out pcr amplification rightly, primer is as follows:
Primer 1:5 '-TAT AGA ATT CGC CAC CAT GTC CTT CAG TGC CAC CAT-3 '
Primer 2: 5 '-TAT AGC CAC CGA ATT CAT GTC CTT CAG TGC CAC CAT-3 '
Description of drawings
Fig. 1 is 1% agarose electrophoresis result behind the PCR.
Fig. 2 is the expression figure (Westernblot) of p60 encoded protein in eukaryotic cell MCF-7.One anti-is mouse anti human Flag monoclonal antibody, and the two anti-anti-mouse horseradish peroxidase of rabbit (HRP) link coupled of preserving for this laboratory two resist.1 negative contrast, 2 is cell pyrolysis liquid.PcDNA3.1 (-)-p60 plasmid transfection MCF-7 cell with band Flag label.Protein level is with anti-Flag antibody test.1, the cell lysates of negative control and MCF-7 is represented in 2 roads respectively.
Fig. 3 is the chromosomal localization of gene, is positioned karyomit(e) 14q11.2.
Embodiment
One aspect of the present invention is the PQ 9H972-2 that can suppress breast cancer cell propagation, and its aminoacid sequence is shown in SEQ ID No.2 in the sequence table.Albumen of the present invention can be recombinant protein, native protein, synthetic proteins etc., preferably recombinant protein.Albumen of the present invention can be the product of natural purifying, or the product of chemosynthesis, or uses recombinant technology to produce from protokaryon or eucaryon host (for example, bacterium, yeast, higher plant, insect and mammalian cell).Albumen of the present invention can be that glycosylation maybe can be non-glycosylated.
Another aspect of the present invention is the above-mentioned proteic gene p60 of coding, and its nucleotide sequence is shown in SEQ ID No.1 in the sequence table.The polynucleotide sequence fragment comprises that its complementary strand can be dna form or rna form.Dna form comprises the DNA of cDNA, genomic dna or synthetic.DNA can be strand or two strands.DNA can be coding strand or noncoding strand.Or the varient of above-mentioned polynucleotide, its coding has the polypeptide of identical aminoacid sequence or fragment, analogue and the derivative of polypeptide with the present invention.This nucleotide diversity body comprises and replaces varient, deletion mutation body and insert varient.
The invention still further relates to the carrier that comprises polynucleotide of the present invention, with the genetically engineered host cell of carrier manufacturing of the present invention and the polypeptide products of the present invention and the method for producing with recombinant technology.Polynucleotide sequence of the present invention can be included in any one of numerous expression vectors of being used for express polypeptide.Suitable carrier for example comprises bacterial plasmid, phage, yeast plasmid, vegetable cell virus, mammalian cell virus as adenovirus, adeno-associated virus, retrovirus or other carrier.The expression vector that is suitable among the present invention can be protokaryon or carrier for expression of eukaryon, as long as can duplicate in host and stablize, any plasmid and carrier can be used.A key character of expression vector is to contain replication orgin, promotor, marker gene and translation controlling elements usually.The carrier that contains gene order of the present invention can be used for transforming or the suitable host cell of transduceing so that this polypeptide of host expresses.
Host cell can be a higher eucaryotic cells, as mammalian cell; Or eukaryotic cell such as low, as yeast cell; Or prokaryotic cell prokaryocyte, for example bacterial cell such as intestinal bacteria.
Utilize conventional gene recombination technology, can utilize polymerized nucleoside acid sequence of the present invention to be used for expressing or producing the protein polypeptide of the genes encoding with inhibition breast cancer cell propagation of reorganization.Comprise: (1) transforms or the transduction proper host cell with the gene p60 (or varient) with inhibition breast cancer cell propagation of the present invention, the recombinant expression vector that perhaps contains this gene p60; (2) host cell of in suitable medium, cultivating; (3) comprise separation, protein purification substratum or the cultured cells from culture.Thereby can mass production polypeptide of the present invention.
Albumen of the present invention has the effect that suppresses breast cancer cell propagation, and it can be used for the treatment of mammary cancer.Therefore, an object of the present invention is above-mentioned albumen of the present invention in preparation treatment tumour, the especially purposes in the mammary cancer.
Albumen of the present invention can be separately or is used in combination with suitable pharmaceutical carrier.This composition can comprise albumen or antagonist and the pharmaceutically acceptable carrier or the excipient for the treatment of significant quantity, and such carrier includes but not limited to: salt solution, buffer saline, glucose, water, glycol, ethanol, or mixture, these preparations should be fit to administering mode.
Pharmaceutical composition of the present invention administration by rights is as by part, intravenously, intraperitoneal, intramuscular, administration such as subcutaneous.The consumption that delivers medicine to the patient depends on many factors, as administering mode, and person's to be treated physical qualification and diagnostician's judgement.
The polypeptide of equivalence can obtain its antibody on albumen of the application of the invention or the immunology, and antibody is used for described proteic detection and purifying.Secondly can utilize albumen of the present invention or its partial amino-acid series to produce antibody originally as immunity.Produce polyclonal antibody by antibody being inoculated in addition, can also produce monoclonal antibody by conventional hybridization knurl method to host animal and the ordinary method that reclaims serum.
The p60 gene is carried out bioinformatic analysis, find that its express spectra is more extensive.
The p60 expressing information:
(1) tissue of high expression level: BM-CD34+, tire brain, cauda equina nerve joint, BM-CD105+ endotheliocyte, marrow, superior cervical ganglion, tonsilla, thalamus, uterus, skeletal muscle, cardiac muscle, pancreas islet, Burkitt lymphomas, 721B lymphoma, PB-CD4+T cell.
(2) low tissue of expressing: gasserian ganglion, hypothalamus, tonsilla, cerebellum, prefrontal cortex, top, full brain, pancreas, sialisterium, prostate gland, suprarenal gland, tire Tiroidina, chronic myelocytic leukemia, Lymphocytic leukemia, PB-CD14+ monocyte.
Therefore, the present invention can promote our understanding to the mammary cancer mechanism, and with this theoretical foundation as diagnoses and treatment.The albumen of this coded by said gene of what is more important can be used for the drug candidate as tumour especially breast cancer treatment.
Embodiment
Embodiment 1:
With PCR method from the proteic p60 gene of human breast carcinoma cDNA library clone coding Q9H972-2.With mammary cancer cDNA library is template, and Flag label on P60 gene N termination carries out pcr amplification with following primer:
Primer 1:5 '-TAT AGA ATT CGC CAC CAT GTC CTT CAG TGC CAC CAT-3 '
Primer 2: 5 '-TAT AGC CAC CGA ATT CAT GTC CTT CAG TGC CAC CAT-3 '
The condition of amplified reaction: in the reaction volume of 50 μ L, contain 50mmol/L KCl, 10mmol/L Tris-C1 (PH8.5), 1.5mmol/L MgCl
2, 200 μ mol/L dNTP, 10pmol primer, the pfu archaeal dna polymerase of 0.25U (Takara company product).Press 30 cycles of following conditioned response: 94 ℃ of 30sec; 56 ℃ of 30sec; 72 ℃ of 2min 30sec.Amplified production is with the test kit purifying of QIAGEN company, behind EcoRI and BamHI double digestion, is cloned in pcDNA3.1 (-) carrier for expression of eukaryon that same enzyme cuts.The dna sequence analysis result shows that the PCR product conforms to substantially with the dna sequence dna of expection.
The expression of embodiment 2:p60 in eukaryotic cell
Western Blot grows up on the basis of protein electrophorese and solid-phase immunoassay, and its ultimate principle is antigen antibody reaction.The cell protein extract is transferred to (nitrocellulose filter is adopted in this experiment) on the solid support after polyacrylamide gel electrophoresis separates.(the anti-people Flag of this laboratory mice monoclonal antibody Sigma), is hatched colour developing with horseradish peroxidase (HRP) link coupled two anti-(it is anti-that the anti-mouse of rabbit two is preserved in this laboratory) again, can obtain the western blot figure of differential protein molecule with special one anti-reaction.This method has detect specific antigenic characteristics from mixes antigen, so this experiment is used to detect the expression behind the p60 gene transfection MCF-7 cell that has the Flag label.
1) transient transfection experiment:
In transfection the day before yesterday with the MCF-7 cell inoculation in the 10cm culture plate, inoculum density is 2 * 10
6Individual cells/well, every hole adds nutrient solution 10ml.After 24 hours, cell grows to when covering the about 85-90% of culture hole floorage, can prepare transfection.The following solution of preparation in the EP pipe: solution A: 24 μ g pcDNA3.1 (-)-p60 expression plasmid are dissolved in the unparalleled anti-DMEM nutrient solution of 1.5mL serum-free.Solution B: 48 μ L liposome solutions (lipofectamine 2000 reagent) are joined in the unparalleled anti-DMEM nutrient solution of 1.5mL serum-free, and room temperature left standstill 5 minutes.Then solution A, B are mixed, room temperature left standstill 20 minutes, with serum-free with do not contain pair DMEM nutrient solution washed cells that resist.
With adding the unparalleled anti-DMEM nutrient solution of 12mL serum-free in the mixed solution of above-mentioned A, B solution, add to cell surface behind the mixing.37 ℃, after the 5%CO2 condition is hatched 4-6 hour, replace aforementioned nutrient solution with the DMEM nutrient solution that normally contains 10% foetal calf serum again, continue culturing cell to 48 hour.
2) extraction of total protein of cell:
Cultured cells makes it to disperse through 0.25% trysinization, and 4 ℃ of low-speed centrifugals remove supernatant, and precipitation is placed 30min on ice, and the PBS with precooling washes per 10 2 times again
6Cell adds lysate 100 μ L, makes the abundant cracking 30min of cell, and again in 4 ℃, the centrifugal 15min of 20000 * g collects supernatant.
3) the Bradford method is measured the proteic concentration of cell pyrolysis liquid:
At first prepare coomassie brilliant blue staining liquid; Standard bovine serum albumin (BSA) is mixed with the protein standard liquid of 1mg/ml, 10 μ g, 20 μ g, 30 μ g, 40 μ g, 50 μ g, 60 μ g protein standard liquid and 5 μ L testing protein quality samples are added in the 3mL Xylene Brilliant Cyanine G dye liquor respectively, incubated at room 10min measures absorption value at 595nm wavelength place.BSA standard model bioassay standard curve, OD within the specific limits
595Be directly proportional with protein concentration.The concentration of sample protein matter is calculated through the typical curve fit equation.
4) electrophoresis and immune response:
Flow process is as follows: A, glue (5% concentrates glue, 10% separation gel); B, 50 μ g protein samples add sample-loading buffer, boil last sample; C, electrophoresis concentrate glue 80V voltage, separation gel 120V voltage; D, commentaries on classics film, 100V, 1.5h; E, sealing, 1h; F, an anti-reaction, are spent the night by 4 ℃; G, TBST wash film, and three times, each 10min; H, two anti-reactions, 1.5h; I, TBST wash film, and three times, each 10min; J, colour developing, exposure.
Embodiment 3:MTT method detects the influence of the Q9H972-2 albumen of different concns to breast cancer cell propagation
MTT (tetramethyl-azo azoles orchid) can be by cellular uptake, and is reduced into a kind of water-fast bluish voilet product first a ceremonial jade-ladle, used in libation by mitochondrial dehydrogenase in the viable cell, and is deposited in the cell, and dead cell does not have this function.The first a ceremonial jade-ladle, used in libation dissolves in dimethyl sulfoxide (DMSO) (DMSO), and solution has maximum absorption at the 570nm place.So the big more representative survivaling cell of this wave band place absorption value amount is many more.Mtt assay is widely used in cytotoxicity experiment, cell growth measurement etc.This experiment utilizes mtt assay to observe the influence of Q9H972-2 albumen to the MCF-7 growth.Concrete operation method is as follows:
In 96 orifice plates, every hole inoculating cell number is 1 * 10 with the MCF-7 cell inoculation of cultivating logarithmic phase
4Individual.Add Q9H972-2 albumen (25,50,100ng) according to concentration gradient next day, every mass action 6 porocytes, and 37 ℃, 5%CO2 cultivates.After cultivating 1d, 2d, 3d, 4d respectively, the MTT solution that in each cell cultures hole, adds 50 μ lL1mg/mL, same culture condition effect down took out after 4 hours, liquid in the careful every hole of sucking-off, every hole adds dimethyl sulfoxide (DMSO) 150 μ L, slight vibration 10 minutes fully is dissolved in the dimethyl sulfoxide (DMSO) bluish voilet crystallization.Measure in 96 orifice plates every hole at the light absorption value at 570nm place with automatic microplate reader.The MTT experiment shows that Q9H972-2 albumen plays restraining effect to the MCF-7 growth and proliferation of cell, and relevant with dosage.
P60 gene [1] .ST25
SEQUENCE?LISTING
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Met?Ser?Phe?Ser?Ala?Thr?Ile?Leu?Phe?Ser?Pro?Pro?Ser?Gly?Ser?Glu
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gcc?aga?tgc?tgc?tgc?tgc?gcc?tgt?aag?agt?gag?act?aat?gga?ggc?aac 96
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Ile?Tyr?Gln?Arg?Val?Asp?Lys?Ala?Val?Gly?Leu?Ala?Glu?Ala?Ala?Leu
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ggt?ctt?gcc?agg?gcc?aac?aat?gag?ttg?tta?aaa?cgt?ctc?cag?gag?gaa 288
Gly?Leu?Ala?Arg?Ala?Asn?Asn?Glu?Leu?Leu?Lys?Arg?Leu?Gln?Glu?Glu
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Val?Gly?Asp?Leu?Arg?Gln?Gly?Lys?Val?Ser?Ile?Pro?Asp?Glu?Asp?Gly
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Glu?Ser?Arg?Ala?His?Ser?Ser?Pro?Pro?Glu?Glu?Pro?Gly?Pro?Leu?Lys
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Glu?Ser?Pro?Gly?Glu?Ala?Phe?Lys?Ala?Leu?Ser?Ala?Val?Glu?Glu?Glu
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Cys?Asp?Ser?Val?Gly?Ser?Gly?Val?Gln?Val?Val?Ile?Glu?Glu?Leu?Arg
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Gln?Leu?Gly?Ala?Ala?Ser?Val?Gly?Pro?Gly?Pro?Leu?Gly?Phe?Pro?Ala
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Thr?Gln?Arg?Asp?Met?Arg?Leu?Pro?Gly?Cys?Thr?Leu?Ala?Ala?Ser?Glu
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Thr?Gly?Glu?Leu?Arg?His?Ser?Leu?Gly?Leu?Thr?Val?Ser?Pro?Cys?Arg
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Phe?Ala?Asn?Arg?Ser?Ser?Ile?Met?Arg?His?Phe?Gly?Pro?Glu?Asp?Gln
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Ser?Leu?Asn?Glu?Pro?Gly?Val?Trp?Val?Ala?Arg?Pro?Pro?Arg?Phe?Arg
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His?Gly?Thr?Lys?Ala?Asn?Arg?Val?Tyr?Gly?Pro?Pro?Ser?Asp?Arg?Leu
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Pro?Ser?Ala?Glu?Ala?Gln?Leu?Leu?Pro?Pro?Glu?Leu?Tyr?Asn?Pro?Asn
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Thr?Gly?Ser?Gln?Gly?Gly?Asn?Pro?Pro?Pro?Ser?Thr?Pro?Ile?Thr?Val
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Thr?Gly?His?Gly?Leu?Ala?Val?Gln?Ser?Ser?Glu?Gln?Leu?Leu?His?Val
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Ile?Tyr?Gln?Arg?Val?Asp?Lys?Ala?Val?Gly?Leu?Ala?Glu?Ala?Ala?Leu
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Gly?Leu?Ala?Arg?Ala?Asn?Asn?Glu?Leu?Leu?Lys?Arg?Leu?Gln?Glu?Glu
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Val?Gly?Asp?Leu?Arg?Gln?Gly?Lys?Val?Ser?Ile?Pro?Asp?Glu?Asp?Gly
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Glu?Ser?Arg?Ala?His?Ser?Ser?Pro?Pro?Glu?Glu?Pro?Gly?Pro?Leu?Lys
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Glu?Ser?Pro?Gly?Glu?Ala?Phe?Lys?Ala?Leu?Ser?Ala?Val?Glu?Glu?Glu
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Cys?Asp?Ser?Val?Gly?Ser?Gly?Val?Gln?Val?Val?Ile?Glu?Glu?Leu?Arg
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Gln?Leu?Gly?Ala?Ala?Ser?Val?Gly?Pro?Gly?Pro?Leu?Gly?Phe?Pro?Ala
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Thr?Gln?Arg?Asp?Met?Arg?Leu?Pro?Gly?Cys?Thr?Leu?Ala?Ala?Ser?Glu
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Ala?Ala?Pro?Leu?Leu?Asn?Pro?Leu?Val?Asp?Asp?Tyr?Val?Ala?Ser?Glu
195 200 205
Gly?Ala?Val?Gln?Arg?Val?Leu?Val?Pro?Ala?Tyr?Ala?Lys?Gln?Leu?Ser
210 215 220
Pro?Ala?Thr?Gln?Leu?Ala?Ile?Gln?Arg?Ala?Thr?Pro?Glu?Thr?Gly?Pro
225 230 235 240
Glu?Asn?Gly?Thr?Lys?Leu?Pro?Pro?Pro?Arg?Pro?Glu?Asp?Met?Leu?Asn
245 250 255
Ala?Ala?Ala?Ala?Leu?Asp?Ser?Ala?Leu?Glu?Glu?Ser?Gly?Pro?Gly?Ser
260 265 270
Thr?Gly?Glu?Leu?Arg?His?Ser?Leu?Gly?Leu?Thr?Val?Ser?Pro?Cys?Arg
275 280 285
Thr?Arg?Gly?Ser?Gly?Gln?Lys?Asn?Ser?Arg?Arg?Lys?Arg?Asp?Leu?Val
290 295 300
Leu?Ser?Lys?Leu?Val?His?Asn?Val?His?Asn?His?Ile?Thr?Asn?Asp?Lys
305 310 315 320
Arg?Phe?Asn?Gly?Ser?Glu?Ser?Ile?Lys?Ser?Ser?Trp?Asn?Ile?Ser?Val
325 330 335
Val?Lys?Phe?Leu?Leu?Glu?Lys?Leu?Lys?Gln?Glu?Leu?Val?Thr?Ser?Pro
340 345 350
His?Asn?Tyr?Thr?Asp?Lys?Glu?Leu?Lys?Gly?Ala?Cys?Val?Ala?Tyr?Phe
355 360 365
Leu?Thr?Lys?Arg?Arg?Glu?Tyr?Arg?Asn?Ser?Leu?Asn?Pro?Phe?Lys?Gly
370 375 380
Leu?Lys?Glu?Lys?Glu?Glu?Lys?Lys?Leu?Arg?Ser?Arg?Arg?Tyr?Arg?Leu
385 390 395 400
Phe?Ala?Asn?Arg?Ser?Ser?Ile?Met?Arg?His?Phe?Gly?Pro?Glu?Asp?Gln
405 410 415
Arg?Leu?Trp?Asn?Asp?Val?Thr?Glu?Glu?Leu?Met?Ser?Asp?Glu?Glu?Asp
420 425 430
Ser?Leu?Asn?Glu?Pro?Gly?Val?Trp?Val?Ala?Arg?Pro?Pro?Arg?Phe?Arg
435 440 445
Ala?Gln?Arg?Leu?Thr?Glu?Leu?Cys?Tyr?His?Leu?Asp?Ala?Asn?Ser?Lys
450 455 460
His?Gly?Thr?Lys?Ala?Asn?Arg?Val?Tyr?Gly?Pro?Pro?Ser?Asp?Arg?Leu
465 470 475 480
Pro?Ser?Ala?Glu?Ala?Gln?Leu?Leu?Pro?Pro?Glu?Leu?Tyr?Asn?Pro?Asn
485 490 495
Phe?Gln?Glu?Glu?Glu?Asp?Glu?Gly?Gly?Asp?Glu?Asn?Ala?Pro?Gly?Ser
500 505 510
Pro?Ser?Phe?Asp?Gln?Pro?His?Lys?Thr?Cys?Cys?Pro?Asp?Leu?Asn?Ser
515 520 525
Phe?Ile?Glu?Ile?Lys?Val?Glu?Lys?Asp?Glu
530 535
Claims (2)
1. aminoacid sequence is the application of gene in the medicine of preparation treatment mammary cancer that SEQ ID NO:2 recombinant protein or nucleotides sequence are classified SEQ ID NO:1 as in the sequence table.
2. a pharmaceutical composition for the treatment of mammary cancer comprises that aminoacid sequence is albumen and the pharmaceutically acceptable carrier of SEQ ID NO:2 in the sequence table.
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AY421032.EMBL.2003,1-6. |
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Q9H972.EMBL.2001,1-3. |
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