CN101053674B - Method for preparing artificial dura meter of brain using with bacterial cellulose - Google Patents
Method for preparing artificial dura meter of brain using with bacterial cellulose Download PDFInfo
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- CN101053674B CN101053674B CN2007100155375A CN200710015537A CN101053674B CN 101053674 B CN101053674 B CN 101053674B CN 2007100155375 A CN2007100155375 A CN 2007100155375A CN 200710015537 A CN200710015537 A CN 200710015537A CN 101053674 B CN101053674 B CN 101053674B
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Abstract
The invention discloses a method of preparaing artificial dura mater by bacteria cellulose, which steps are crushing moisture film of bacteria cellulose to get bacteria cellulose homogenate; blending the bacteria cellulose homogenate and polyvinyl alcohol solution with 7%-15% concentration in ratio of 1:1-10, water content of the bacteria cellulose homogenate is 95%-98%; then spreading the mixture in a mould with thickness is 0.26 mm-0.90 mm, freezing 20-30 hours in condition of -20--4 DEG C; drying in vacuum freeze dryer to get formed artificial dura mater. The artificial dura mater of the invention have good elasticity and flexibility in hygrometric state, experimental detecting: commonly thickness is 0.18-0.78 mm, water seepage rate is 0, breaking tenacity is 3-7 N/mm2, extensibilityat break is 30-98%, pawn strength is 3-7 N/mm2, which is uncapable of tearing and frilling when sewing. The method of the invention has environmental protective production process, simple production technics, low cost, is suit for spreading.
Description
Technical field
The present invention relates to a kind of implantation or repair the human organ preparation methods, relate in particular to a kind of method of utilizing Bacterial cellulose to prepare artificial dura mater.
Background technology
Clinically, people's cerebral dura mater is normal because the destruction of the erosion of open injury, tumor, inflammation and other brain disorder are former thereby it is damaged to cause, and needs in time to repair in case cerebrospinal fluid is excessive complication such as intracranial infection.It repairs succedaneum freezing human cerebral dura mater, but its source is limited; The biomembrane that useful pig peritoneum, fetal membrane, amniotic membrane etc. get through special the processing, the artificial meninges that perhaps uses simple bioprotein to make, as the collagen protein artificial dura mater, the defective of this series products is easily cerebral tissue to be produced the abnormal pigmentary deposit on the skin trace, stimulate, can be absorbed fully, be unfavorable for post surgery treatment, therefore, it is very necessary and urgent developing ideal cerebral dura mater substitute.
Bacterial cellulose was found by Brown in 1886, acetobacter xylinum (Gluconacetobacter xylinum) forms one deck white fiber shape material in media surface when static cultivation, determine that through chemistry and physical method analysis this type of material has cellulosic structure and chemical property, because of belonging to antibacterial, it synthesizes, so the called after Bacterial cellulose (bacterial cellulose, BC).The Bacterial cellulose diameter only is 1/10 of artificial synthetic fibers, is 10nm～100nm, is a kind of novel nano grade biological material.It has the character of many uniquenesses, as high-crystallinity and high chemical purity, and high-tensile and elastic modelling quantity, very strong water associativity, splendid shape maintains ability and anti-tear power, higher biocompatibility and good biodegradability.So the research and development of this new bio nano material are subjected to the attention of various countries' researcher day by day.But, in the existing relevant research of Bacterial cellulose, utilize Bacterial cellulose to prepare the research and the correlation technique of cerebral dura mater substitute, yet there are no report by retrieval.
Summary of the invention
At the deficiencies in the prior art, the purpose of this invention is to provide a kind of method of utilizing Bacterial cellulose to prepare artificial dura mater.This method can utilize Bacterial cellulose to prepare the cerebral dura mater substitute, and improving the using value of Bacterial cellulose, and properties of product and biological tissue's compatibility are good, nontoxic, and material processing technique is simple.
The method of utilizing Bacterial cellulose to prepare artificial dura mater of the present invention, step is:
(1) bacterial cellulose wet-coating is cut into 0.5cm
2～4cm
2Fritter, place the container that fills water, adopt ultrasound wave or mechanical system fragmentation, Bacterial cellulose homogenate;
(2) water content of mensuration Bacterial cellulose homogenate;
(3) water soluble polymer auxiliary pva (PVA) being mixed with mass concentration is 7%～15% solution;
(4) water content in Bacterial cellulose homogenate is 95%～98%, is 7%～15% poly-vinyl alcohol solution with described Bacterial cellulose homogenate and concentration according to 1: 1～10 ratio uniform mixing;
(5) mixture is layered in the mould uniformly, and to make its thickness be 0.26mm～0.90mm, placed then under-20 ℃～-4 ℃ conditions freezing 20 hours～30 hours;
(6) be placed on the interior lyophilizing of vacuum freeze drier 20 hours～24 hours, the artificial dura mater that must be shaped then.
Wherein: the described bacterial cellulose wet-coating of step (1) is prepared by following method:
Bacterial strain is selected: select acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.1812 or acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.2378; Seed and fluid medium preparation: by weight percentage, at the glucose or fructose or the sucrose that contain 2%～4%, 0.5%～1% yeast powder, 0.5%～1% tryptone, 0.5%～1%Na
2HPO
40.2%～0.3% citric acid, add the sodium alginate of 0.2～0.8g/L in the seed culture medium of 2%～3% calcium carbonate or the fluid medium or do not add, the pH value of regulating culture medium then is 5～6,20～the 25min that sterilizes under 121 ℃ temperature makes and contains sodium alginate seed and fluid medium, after being cooled to 30 ℃, standby; Seed cell is cultivated: get the good inclined-plane seed of 1～2 ring activation and insert and contain in the fluid medium of sodium alginate, 8 ℃～32 ℃ shaken cultivation 24 hours～36 hours, shaking speed is 140～180r/min, seed liquor; Static liquid fermented-producing bacteria cellulose: be that 5%～10% inoculum concentration is inoculated into seed liquor in the fluid medium that contains sodium alginate with percent by volume, fully vibration makes bacterium liquid even, 28 ℃～32 ℃ leave standstill cultivation 6～10 days, have the bacteria cellulose film of generation to float on liquid level; Bacteria cellulose film purification: the bacteria cellulose film of getting generation, water flushing 6～10 times, remove striping surface medium and impurity, film is soaked in the aqueous slkali of 0.08～0.12M, 80 ℃～100 ℃ are boiled 20min～30min again, remove thalline and residual culture medium in the film, film be creamy white translucent after, with distilled water flushing and survey film pH value to 7.0～7.2 o'clock, flushing stops, bacterial cellulose wet-coating.
In the above-mentioned method for preparing bacterial cellulose wet-coating, preferred embodiment be:
Described seed and fluid medium preparation are by weight percentage, contain 2% glucose or fructose or sucrose, 0.5% yeast powder, 0.5% tryptone, 0.5%Na
2HPO
4, 0.2% citric acid adds the sodium alginate of 0.4～0.6g/L in the seed culture medium of 2% calcium carbonate or the fluid medium, and the pH value of regulating culture medium then is 6.
It is to get the good inclined-plane seed of 1～2 ring activation to insert and contain in the fluid medium of sodium alginate that described seed cell is cultivated, 30 ℃～32 ℃ shaken cultivation 24 hours～26 hours, shaking speed is 150～160r/min, seed liquor.
Described static liquid fermented-producing bacteria cellulose is to be that 6%～8% inoculum concentration is inoculated into seed liquor in the fluid medium that contains sodium alginate with percent by volume, fully vibration makes bacterium liquid even, 30 ℃～32 ℃ leave standstill cultivation 6～8 days, have the bacteria cellulose film of generation to float on liquid level.
Described bacteria cellulose film purification is a bacteria cellulose film of getting generation, water flushing 8～10 times, remove striping surface medium and impurity, again film is soaked in the aqueous slkali of 0.1M, 100 ℃ are boiled 20min, remove thalline and residual culture medium in the film, film be creamy white translucent after, with distilled water flushing and survey film pH value to 7.0～7.2 o'clock, flushing stops.
Wherein: described aqueous slkali is NaOH solution or Na
2CO
3Solution.
In seed culture medium of the present invention and the fluid medium mainly by 20～30g/L carbohydrate raw material, 5～10g/L nitrogen-containing material, 1～1.5g/L antistain agent, 0.2～0.6g/L additive is formed, above-mentioned raw materials is added the water heating for dissolving, and pH regulator to 5～6 can obtain.
Wherein: carbohydrate mainly comprises fructose, glucose, sucrose or Sucus Cocois etc., and nitrogen-containing material mainly refers to yeast extract or peptone etc., and additive mainly is meant sodium alginate.
Described pH regulator mainly adopts citric acid, acetic acid or Na
2HPO
4Or K
2HPO
4Or KH
2PO
4
The above-mentioned utilization in the method that Bacterial cellulose prepares artificial dura mater preferred embodiment is:
The described broken homogenizer fragmentation of adopting of step (1), treatment conditions are 10000～12000 rev/mins, 2～3min.
The described polyvinyl alcohol molecular weight of step (3) is 2.5～150,000, and the degree of polymerization is 500～2000, and alcoholysis degree is 85-99%; The mass concentration of described polyvinyl alcohol (PVA) solution is 10%.
Described Bacterial cellulose homogenate of step (4) and concentration are that 7%～15% the blended ratio of poly-vinyl alcohol solution is 1: 5～8.More preferably: 1: 7.
The described freezing conditions of step (5) is-18 ℃～-15 ℃.
Make a kind of novel biomaterial as artificial dura mater with the water soluble polymer auxiliary pva is compound after the bacterial cellulose wet-coating homogenate that the present invention generates acetobacter xylinum fermentation, Bacterial cellulose is a nano-scale fiber, add polyvinyl alcohol and kept its high strength, improve its anti-stitching again, make the artificial dura mater of making have following characteristics: 1, have reliable hardness and good pliability, elasticity; When surgical repair and after repairing, certain power can be born, distortion can be produced and not damaged.2, has good biological tissue's compatibility, no foreign body rejection.3, can not be absorbed fully; Can play the effect of protection cerebral tissue, can play a part again to isolate scalp soft tissue and cerebral tissue well, make later cranioplasty surgery safer, reliable.4, prevent or alleviate partial adhesion in operation back and cicatrization, the generation of prevention epilepsy.5, prevent the generation of cerebrospinal leak.
The present invention utilizes Bacterial cellulose to prepare the method for artificial dura mater, the production process environmental protection, and production technology is simple, and cost is low, is suitable for promoting.
The present invention utilizes the artificial dura mater of Bacterial cellulose preparation that better elastic and pliability are arranged when hygrometric state, and measuring: thickness is generally 0.18～0.78mm, and seepage rate is 0, and fracture strength is 3～7N/mm
2, elongation at break is 30～98%, colluding is by force 3～7N/mm
2Do not tear during stitching, not off-clip.
The specific embodiment
Embodiment 1
(1) bacterial strain is selected: select acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.1812.
(2) contain the preparation of sodium alginate seed and fluid medium: by weight percentage, contain 2% fructose, 0.5% yeast powder, 0.5% tryptone, 0.5%Na
2HPO
4, 0.2% citric acid adds the sodium alginate of 0.5g/L in the seed culture medium of 2% calcium carbonate or the fluid medium, the pH value of regulating culture medium then is 6, the 20min that under 121 ℃ temperature, sterilizes, be cooled to 30 ℃ after, standby;
(3) seed cell is cultivated: good inclined-plane seed (acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.1812) access contains in the fluid medium of sodium alginate to get 1～2 ring activation, 30 ℃ of shaken cultivation 24 hours, shaking speed is 160r/min, gets seed liquor;
(4) static liquid fermented-producing bacteria cellulose: be that 6% inoculum concentration is inoculated into seed liquor in the fluid medium that contains sodium alginate with percent by volume, fully vibration makes bacterium liquid even, 30 ℃ leave standstill cultivation 8 days, have the bacteria cellulose film of generation to float on liquid level.
(5) bacteria cellulose film purification: the bacteria cellulose film of getting generation, water flushing 8～10 times, remove striping surface medium and impurity, film is soaked in the NaOH solution of 0.1M, 100 ℃ are boiled 20min again, remove thalline and residual culture medium in the film, film be creamy white translucent after, with distilled water flushing and survey film pH value to 7.0～7.2 o'clock, flushing stops, bacterial cellulose wet-coating.
(6) bacterial cellulose wet-coating is cut into 0.5cm
2～4cm
2Fritter, place the container that fills water, adopt ultrasonic disruption, Bacterial cellulose homogenate;
(7) water content of mensuration Bacterial cellulose homogenate is 95% (V/V);
(8) water soluble polymer auxiliary pva (PVA, molecular weight are 3.5 ten thousand, and the degree of polymerization is 600, and alcoholysis degree is 97%) being mixed with mass concentration is 15% solution;
(9) water content in Bacterial cellulose homogenate is 95%, is 15% poly-vinyl alcohol solution with described Bacterial cellulose homogenate and concentration according to 1: 2 ratio uniform mixing;
(10) mixture is layered in the mould uniformly, and to make its thickness be 0.26mm～0.90mm, placed then under-20 ℃ of conditions freezing 20 hours;
(11) be placed on the interior lyophilizing of vacuum freeze drier 24 hours, the artificial dura mater that must be shaped then.
Artificial dura mater thickness is 0.366mm, and being cut into 30mm * 5mm small pieces mensuration fracture strength is 4.236N/mm
2With elongation at break be 30%, will cut off in the middle of the above-mentioned material, the end place is sewed up with 4 trumpeter's art round needle single lines, measures that to collude be by force 4.222N/mm
2
Embodiment 2
(1) bacterial strain is selected: select acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.2378.
(2) seed and fluid medium preparation: by weight percentage, the sucrose with 4%, 1% yeast powder, 1% tryptone, 1%Na
2HPO
4, 0.3% citric acid, 3% calcium carbonate is prepared into seed culture medium or fluid medium, the pH value of regulating culture medium then is 5.3, the 20min that under 121 ℃ temperature, sterilizes, be cooled to 30 ℃ after, standby;
(3) seed cell is cultivated: get the good inclined-plane seed (acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.2378) of 1～2 ring activation and insert in the fluid medium, 32 ℃ of shaken cultivation 24 hours, shaking speed is 180r/min, gets seed liquor;
(4) static liquid fermented-producing bacteria cellulose: be that 6% inoculum concentration is inoculated into seed liquor in the fluid medium with percent by volume, fully vibration makes bacterium liquid even, and 30 ℃ leave standstill and cultivated 8 days, have the bacteria cellulose film of generation to float on liquid level.
(5) bacteria cellulose film purification: get the bacteria cellulose film of generation, water flushing 10 times removes striping surface medium and impurity, film is soaked in the Na of 0.12M again
2CO
3In the solution, 100 ℃ are boiled 30min, remove thalline and residual culture medium in the film, film be creamy white translucent after, with distilled water flushing and survey film pH value to 7.0～7.1 o'clock, flushing stops, bacterial cellulose wet-coating.
(6) bacterial cellulose wet-coating is cut into small pieces, places homogenizing in the homogenizer, revolution is 12000 rev/mins, and the time is 2min, obtains Bacterial cellulose homogenate, and the water content of measuring Bacterial cellulose is 98% (V/V).It is 10% solution that PVA (molecular weight is 50,000, and the degree of polymerization is 1000, and alcoholysis degree is 95%) is mixed with mass concentration, with Bacterial cellulose homogenate and 10%PVA according to 1: 7 mix homogeneously.Mixture is layered in the culture dish that diameter is 90mm uniformly, and thickness is about 0.46mm, carries out 24 hours film forming of lyophilization after placing-18 ℃ of refrigerators freezing.
Embodiment 3
(1) bacterial strain is selected: select acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.1812.
(2) contain the preparation of sodium alginate seed and fluid medium: by weight percentage, contain 3% glucose, 0.8% yeast powder, 0.7% tryptone, 0.8%Na
2HPO
4, 0.25% citric acid adds the sodium alginate of 0.7g/L in the seed culture medium of 2.5% calcium carbonate or the fluid medium, the pH value of regulating culture medium then is 5.7, the 20min that under 121 ℃ temperature, sterilizes, be cooled to 30 ℃ after, standby;
(3) seed cell is cultivated: good inclined-plane seed acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.1812 access contains in the fluid medium of sodium alginate to get 1～2 ring activation, 22 ℃ of shaken cultivation 36 hours, shaking speed is 150r/min, gets seed liquor;
(4) static liquid fermented-producing bacteria cellulose: be that 5% inoculum concentration is inoculated into seed liquor in the fluid medium that contains sodium alginate with percent by volume, fully vibration makes bacterium liquid even, 28 ℃ leave standstill cultivation 10 days, have the bacteria cellulose film of generation to float on liquid level.
(5) bacteria cellulose film purification: the bacteria cellulose film of getting generation, water flushing 10 times, remove striping surface medium and impurity, film is soaked in the NaOH solution of 0.09M, 80 ℃ are boiled 30min again, remove thalline and residual culture medium in the film, film be creamy white translucent after, with distilled water flushing and survey film pH value to 7.1～7.2 o'clock, flushing stops, bacterial cellulose wet-coating.
(6) bacterial cellulose wet-coating is cut into 0.5cm
2～4cm
2Fritter, place the container that fills water, adopt the homogenizer fragmentation, treatment conditions are 12000 rev/mins, 2min, Bacterial cellulose homogenate;
(7) water content of mensuration Bacterial cellulose homogenate is 97% (V/V);
(8) water soluble polymer auxiliary pva (molecular weight is 100,000, and the degree of polymerization is 1000, and alcoholysis degree is 99%) being mixed with mass concentration is 7% solution;
(9) water content in Bacterial cellulose homogenate is 97%, is 7% poly-vinyl alcohol solution with described Bacterial cellulose homogenate and concentration according to 1: 10 ratio uniform mixing;
(10) mixture is layered in the mould uniformly, and to make its thickness be 0.26mm～0.90mm, placed then under-4 ℃ of conditions freezing 30 hours;
(11) be placed on the interior lyophilizing of vacuum freeze drier 20 hours, the artificial dura mater that must be shaped then.
Embodiment 4
Utilize Bacterial cellulose to prepare the method for artificial dura mater, step is:
(1) bacterial cellulose wet-coating is cut into 0.5cm
2～4cm
2Fritter, place the container that fills water, adopt the homogenizer fragmentation, treatment conditions are 10000 rev/mins, 3min, Bacterial cellulose homogenate;
(2) water content of mensuration Bacterial cellulose homogenate is 98% (V/V);
(3) water soluble polymer auxiliary pva (molecular weight is 150,000, and the degree of polymerization is 1500, and alcoholysis degree is 85%) being mixed with mass concentration is 12% solution;
(4) water content in Bacterial cellulose homogenate is 98%, is 12% poly-vinyl alcohol solution with described Bacterial cellulose homogenate and concentration according to 1: 8 ratio uniform mixing;
(5) mixture is layered in the mould uniformly, and to make its thickness be 0.26mm～0.90mm, placed then under-14 ℃ of conditions freezing 25 hours;
(6) be placed on the interior lyophilizing of vacuum freeze drier 22 hours, the artificial dura mater that must be shaped then.
Claims (9)
1. method of utilizing Bacterial cellulose to prepare artificial dura mater, step is:
(1) bacterial cellulose wet-coating is cut into 0.5cm
2～4cm
2Fritter, place the container that fills water, adopt ultrasound wave or mechanical system fragmentation, Bacterial cellulose homogenate;
(2) water content of mensuration Bacterial cellulose homogenate;
(3) the water soluble polymer auxiliary pva being mixed with mass concentration is 7%～15% solution;
(4) water content in Bacterial cellulose homogenate is 95%～98%, is 7%～15% poly-vinyl alcohol solution with described Bacterial cellulose homogenate and concentration according to 1: 1～10 ratio uniform mixing;
(5) mixture is layered in the mould uniformly, and to make its thickness be 0.26mm～0.90mm, placed then under-20 ℃～-4 ℃ conditions freezing 20 hours～30 hours;
(6) be placed on the interior lyophilizing of vacuum freeze drier 20 hours～24 hours, the artificial dura mater that must be shaped then;
Wherein: the described bacterial cellulose wet-coating of step (1) is prepared by following method:
Bacterial strain is selected: select acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.1812 or acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.2378; Seed and fluid medium preparation: by weight percentage, at the glucose or fructose or the sucrose that contain 2%～4%, 0.5%～1% yeast powder, 0.5%～1% tryptone, 0.5%～1%Na
2HPO
40.2%～0.3% citric acid, add the sodium alginate of 0.2～0.8g/L in the seed culture medium of 2%～3% calcium carbonate or the fluid medium, the pH value of regulating culture medium then is 5～6,20～the 25min that sterilizes under 121 ℃ temperature makes seed and fluid medium, after being cooled to 30 ℃, standby; Seed cell is cultivated: get the good inclined-plane seed of 1～2 ring activation and insert and contain in the fluid medium of sodium alginate, 8 ℃～32 ℃ shaken cultivation 24 hours～36 hours, shaking speed is 140～180r/min, seed liquor; Static liquid fermented-producing bacteria cellulose: be that 5%～10% inoculum concentration is inoculated into seed liquor in the fluid medium that contains sodium alginate with percent by volume, fully vibration makes bacterium liquid even, 28 ℃～32 ℃ leave standstill cultivation 6～10 days, have the bacteria cellulose film of generation to float on liquid level; Bacteria cellulose film purification: the bacteria cellulose film of getting generation, water flushing 6～10 times, remove striping surface medium and impurity, film is soaked in the aqueous slkali of 0.08～0.12M, 80 ℃～100 ℃ are boiled 20min～30min again, remove thalline and residual culture medium in the film, film be creamy white translucent after, with distilled water flushing and survey film pH value to 7.0～7.2 o'clock, flushing stops, bacterial cellulose wet-coating.
2. utilize Bacterial cellulose to prepare the method for artificial dura mater according to claim 1, it is characterized in that: described seed and fluid medium preparation are by weight percentage, contain 2% glucose or fructose or sucrose, 0.5% yeast powder, 0.5% tryptone, 0.5%Na
2HPO
4, 0.2% citric acid adds the sodium alginate of 0.4～0.6g/L in the seed culture medium of 2% calcium carbonate or the fluid medium, and the pH value of regulating culture medium then is 6.
3. utilize Bacterial cellulose to prepare the method for artificial dura mater according to claim 1, it is characterized in that: it is to get 1～2 ring activation inclined-plane seed access well to contain in the fluid medium of sodium alginate that described seed cell is cultivated, 30 ℃～32 ℃ shaken cultivation 24 hours～26 hours, shaking speed is 150～160r/min, gets seed liquor.
4. utilize Bacterial cellulose to prepare the method for artificial dura mater according to claim 1, it is characterized in that: described static liquid fermented-producing bacteria cellulose is to be that 6%～8% inoculum concentration is inoculated into seed liquor in the fluid medium that contains sodium alginate with percent by volume, fully vibration makes bacterium liquid even, 30 ℃～32 ℃ leave standstill cultivation 6～8 days, have the bacteria cellulose film of generation to float on liquid level.
5. utilize Bacterial cellulose to prepare the method for artificial dura mater according to claim 1, it is characterized in that: described bacteria cellulose film purification is a bacteria cellulose film of getting generation, water flushing 8～10 times, remove striping surface medium and impurity, film is soaked in the aqueous slkali of 0.1M again, 100 ℃ are boiled 20min, remove thalline and residual culture medium in the film, film be creamy white translucent after, with distilled water flushing and survey film pH value to 7.0～7.2 o'clock, flushing stops.
6. utilize Bacterial cellulose to prepare the method for artificial dura mater according to claim 1, it is characterized in that: described aqueous slkali is NaOH solution or Na
2CO
3Solution.
7. utilize Bacterial cellulose to prepare the method for artificial dura mater according to claim 1, it is characterized in that: the described broken homogenizer fragmentation of adopting of step (1), treatment conditions are 10000～12000 rev/mins, 2～3min.
8. utilize Bacterial cellulose to prepare the method for artificial dura mater according to claim 1, it is characterized in that: the described polyvinyl alcohol molecular weight of step (3) is 2.5～150,000, and the degree of polymerization is 500～2000, and alcoholysis degree is 85-99%; The mass concentration of described poly-vinyl alcohol solution is 10%.
9. utilize Bacterial cellulose to prepare the method for artificial dura mater according to claim 1, it is characterized in that: described Bacterial cellulose homogenate of step (4) and concentration are that 7%～15% the blended ratio of poly-vinyl alcohol solution is 1: 5～8.
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| CN102210888A (en) * | 2010-04-08 | 2011-10-12 | 上海交通大学医学院附属第九人民医院 | Agarose-enhanced three-dimensional nanometer porous bacterial cellulose stent and application thereof |
| CN101948597B (en) * | 2010-09-03 | 2012-05-23 | 东华大学 | Method for preparing bacterial cellulose/polyvinyl alcohol composite membrane by wet process |
| CN102000357A (en) * | 2010-11-26 | 2011-04-06 | 天津信睿生物科技有限公司 | Preparation method of artificial endocranium |
| CN102671236B (en) * | 2012-05-03 | 2014-10-15 | 北京科技大学 | Method for preparing nanofiber reinforcement hydrogel bionic artificial meniscus composite material |
| CN110327135B (en) * | 2019-08-29 | 2019-12-20 | 上海白衣缘生物工程有限公司 | Biological endocranium patch and preparation method thereof |
| CN111420123B (en) * | 2020-03-16 | 2021-12-07 | 江西光至金辉医疗制品有限公司 | Degradable anti-adhesion double-layer dura mater patch and preparation method thereof |
| CN113069590B (en) * | 2021-03-02 | 2022-07-01 | 西北师范大学 | Preparation method of regenerated bacterial cellulose composite hydrogel dressing |
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| EP1660670A1 (en) * | 2003-07-03 | 2006-05-31 | Politechnika Lodzka | A method for the production of bacterial cellulose |
| CN1840677A (en) * | 2006-01-24 | 2006-10-04 | 李胜 | Method for preparing bacteria cellulose |
| CN1850302A (en) * | 2006-05-19 | 2006-10-25 | 东华大学 | Method for preparing bacterial cellulose wet-coating for artificial skin |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1660670A1 (en) * | 2003-07-03 | 2006-05-31 | Politechnika Lodzka | A method for the production of bacterial cellulose |
| CN1840677A (en) * | 2006-01-24 | 2006-10-04 | 李胜 | Method for preparing bacteria cellulose |
| CN1850302A (en) * | 2006-05-19 | 2006-10-25 | 东华大学 | Method for preparing bacterial cellulose wet-coating for artificial skin |
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