CN101052646B - Cell cycle phase markers - Google Patents

Abstract

The present invention relates to polypeptide and nucleic acid construct, it can be used for the Cell cycle status determining mammalian cell.Can be used for determining the impact of test agent on mammalian cell cycle with the host cell of these nucleic acid construct transfections.

Description

Cell cycle phase markers

Technical field

The present invention relates to cell cycle phase Specific marker and the method for determining the transformation in mammalian cell between cell cycle different steps.

Background of invention

Eukaryotic cell division was undertaken by the cell cycle comprising the successive stage being called G1, S, G2 and M of high degree of controlled.The destruction that cell cycle or cell cycle control can cause cellular abnormality or morbid state such as cancer, and this results from multiple hereditary change, and the cell transformation of growth restriction has been become the cell of very invasive by it, and they are not replied the normal control of growth.Normal cell to cancer cells transformation by the correct function of DNA replication dna and DNA repair mechanism forfeiture and produce.Cell in all divisions is all controlled by many controlling mechanisms, is called cell cycle chechpoint, and it is complete by stagnating abnormal cells or inducing paracytic destruction to maintain genome.Therefore the research of cell cycle progress and control is have suitable importance (Flatt, P.M. and Pietenpol, J.A.Drug Metab.Rev., (2000), 32 (3-4), 283-305 for design cancer therapy drug; Buolamwini, J.K.Current PharmaceuticalDesign, (2000), 6,379-392).

Cell cycle progress is strictly controlled by specified time of many Cycle Regulation agent and space expression, location and destroying, they present dynamic (Pines, J., the Nature Cell Biology of height during the cell cycle, (1999) 1, E73-E79).Such as, at specific cell cycle phase, some protein are from nuclear translocation to tenuigenin, or on the contrary, and some protein are degraded rapidly.Understand known cell cycle control composition and interactional details, see Kohn, Molecular Biology of the Cell (1999), 10,2703-2734.

Accurately the determining of Cell cycle status studies the key request of cell processes affecting the cell cycle or depend on cell cycle position.These are determined in drug screening application is vital especially, wherein:

I) material directly or indirectly changing cell cycle progress is needed, such as, for the research as potential anticancer therapy;

Ii) harmful effect of drug candidate cell cycle progress is checked; And/or

Iii) suspect that the cell of reagent to the specific cells phase of the cycles is active or non-activity.

Traditionally, the Cell cycle status of cell mass is determined by flow cytometry, wherein makes the nuclear DNA (Barlogie of fluorochromine, B.et al, Cancer Res., (1983), 43 (9), 3982-97).Flow cytometry produces the quantitative information of cell DNA content, therefore, it is possible to determine the relative cell numbers of G1, S and G2+M phase being in the cell cycle.But this analysis is destructive non-dynamic process, need the serial sampling of cell mass to determine time dependent Cell cycle status.More shortcomings of Flow Cytometry relate to according to DNA content indirectly with the cell cycle position of the determination cell of inferential.Because nuclear DNA content changed in quite expected mode in the whole cell cycle, namely the cell being in G2 or M has the DNA content of G1 cell twice, be in the DNA that cell that the S phase carries out DNA synthesis has intermediate quantity, it is possible for therefore monitoring the Relative distribution of cell between cell cycle different steps.But, this technology accurately can not determine the cell cycle position of any individual cells, this be uncertainty owing to cell to be attributed to G2 or the M phase and due in cell mass between cell and cell the intrinsic difference of DNA content cause further inaccurate, this may eliminate the accurate differentiation between cell that the boundary between the phase adjacent with the cell cycle is close.In addition, this technology of difference requirements of DNA content between the different cell types of different tissues or biology and DNA dyeing is optimized often kind of cell type, immediate data more complicated (the Herman between cell type or between experiment may be made, Cancer (1992), 69 (6), 1553-1556).Therefore flow cytometry is suitable for the whole cell period profile checking cell in cell mass, but can not be used for monitoring the time dependent precise cellular cycle status of individual cells.

EP 798386 describes the method for the cell cycle for analyzing the cell subsets existed in heterogeneous cell sample.The method uses with the continuous incubated samples of fluorescently-labeled monoclonal antibody to differentiate particular cell types and the fluorescence dye of specific binding nucleic acid.This makes it possible to the cell cycle distribution of the cell subsets determining to exist in sample.But, owing to this process employs flow cytometry, therefore it only produces non-dynamic data, need after being exposed to studied reagent, independent cell sample carries out METHOD FOR CONTINUOUS DETERMINATION to determine the Cell cycle status of cell mass over time, to determine the impact that cell cycle is in progress.

Many investigators employed need cell to fix or the routine report enzyme of molten born of the same parents to study the cell cycle.Such as, Hauser & Bauer (Plant and Soil, (2000), 226,1-10) employ β-glucuronidase (GUS) and study cell fission in plant meristematic tissue, Brandeis & Hunt (EMBO J., (1996), 15,5280-5289) employ E.C. 2.3.1.28 (CAT) fusion rotein to study the change of cyclin white level.US6048693 describes the method for screening the compound affecting cyclin, wherein the expression of reporter gene is connected with controlling elements, and these elements are worked by cyclin or other cell cycle control proteins.In the method, it is driven in the mode that the cell cycle is special that the timeliness of reporter gene product is expressed, and the compound acting on one or more cell cycle control compositions can increase or reduce expression level.

US 6159691 describes the nuclear localization signal (NLS) being derived from cell cycle phase idiosyncratic transcription factor DP-3 and E2F-1, the method for the claimed presumption conditioning agent for analysis of cells cycle progress.In the method, the nuclear localization signal (NLS) being derived from cell cycle phase idiosyncratic transcription factor DP-3 and E2F-1 can be used for analysis of compounds activity, and this compound is used for increasing or reducing the nuclear location being fused to the specific NLS sequence from DP-3 and E2F-1 on detectable label.

Jones et al (Nat Biotech., (2004), 23,306-312) describes mitotic biological sensor, and it is based on plasma membrane target signal and the SV40 large T antigen NLS be fused on EYFP.In the whole cell cycle, reporter gene is all arranged in nucleus, but during mitotic division, nuclear membrane decompose and again formed between translocated on plasma membrane.

WO 03/031612 describes DNA and reports construct and the method for cell cycle position for mammalian cell of determining to live, and it relies on the specific expressed controlling elements of cell cycle phase and destruct limit element.

Gu et al. (Mol Biol Cell., 2004,15,3320-3332) have studied the function of people's DNA helicase B (HDHB) recently, show it in the G1 phase substantially at nucleus, in S and the G2 phase substantially at tenuigenin, DNA damage induces it to be positioned at epipole, this epipole pattern needs HDHB active, and HDHB location controls by CDK phosphorylation.

Aforesaid method does not all clearly describe can be used to indicate the sensor of G1, S and G2 phase of cell cycle in stable integration to genome.Therefore, need a kind of method, it makes these cell cycle phases non-destructively can be determined in single mammalian cell alive, same cell can be detected repeatedly in time, and make it possible to study the impact of reagent that cell cycle has potential expectation or undesirably affect.Also the method can carrying out parallel evaluation on these impacts of plurality of reagents is needed.

Summary of the invention

The invention describes a kind of method, the means that it utilizes the key component of cell cycle controlling mechanism to provide new with the combination of regulation, to provide the nondestructive process of dynamic read to determine the Cell cycle status of individual viable cell.

The present invention further provides protein, DNA construct, carrier and express the stable cell lines of these protein, they present the transposition that can detect reporter molecule with cell cycle phase specificity pattern, it is by being directly connected to G1/S cell cycle phase dependency setting control sequence by report signal.This greatly improves the accuracy determining cell cycle phase state, and makes it possible to face the cell cycle progress surveyed in individual cells continuously.And, have been found that and can be separated by the functional element of cell cycle controlling mechanism and extract crucial controlling elements, this makes it possible to design dynamically controlled cell cycle phase reporter molecule, but and as one man can operate independently with endogenous cell periodic Control composition, thus the means provided for monitoring Cell cycle status, its do not affect or the interference cell cycle be naturally in progress.

According to a first aspect of the invention, provide polypeptide construct, it comprises detectable live cell reporter molecule, this molecule is less than 112 by having, the group of 000 Dalton molecular weight is connected at least one cell cycle phase dependency positioning controling element, described element be positioned at G1 and the S phase during change, the transposition of wherein said construct in mammalian cell shows cell cycle position.

It being understood that transposition is defined as reporter molecule and can moves to another with detecting from a Subcellular Localization, typically from nucleus to tenuigenin or contrary.To further understand that, when relating to reporter molecule, term " viable cell " defines the reporter molecule producing detectable signal in viable cell, or the reporter molecule that such as antigenic markers is such, it expresses and can be detected after fixing by immunological method in viable cell, so just be suitable in imaging system, such as IN Cell Analyzer (GE Healthcare).

Suitably, described group has and is less than 100,000 daltonian molecular weight.

Suitably, group has and is less than 50,000 daltonian molecular weight.

Suitably, group has and is less than 25,000 daltonian molecular weight.

Suitably, group has and is less than 10,000 daltonian molecular weight.

Suitably, group has and is less than 1,000 daltonian molecular weight.

Suitably, group has and is less than 700 daltonian molecular weight.

Suitably, group has and is less than 500 daltonian molecular weight.

Preferably, group is polypeptide.Peptide group should be relatively little, and comprise the amino acid allowing reporter molecule to have flexibility relative to cell cycle phase dependency positioning controling element and/or can rotate.More preferably, peptide group is seven peptides.Most preferably, described seven peptide groups are Gycine-Asparagine-Gly-Gly-Asparagine-Alanine-Serines (GNGGNAS).As mentioned above, any amino acid allowing reporter molecule to have flexibility relative to positioning controling element and/or can rotate can be used in polypeptide.

Suitably, cell cycle phase specific localization controlling elements is selected from the peptide group be made up of Rag2, Chaf1B, Fen1, PPP1R2, helicase B, sgk, CDC6 or its motif, the phosphorylation dependency subcellular localization domain (PSLD) in the C-terminal Special controlling district (C-terminal specialcontrol region) of described motif such as helicase B.Known helicase B causes not controlled DNA license and may be harmful to cell survival when overexpression.Therefore, preferably, cell cycle phase dependency positioning controling element is the phosphorylation dependency subcellular localization domain (PSLD) in the C-terminal Special controlling district of helicase B.

Report and characterized people's helicase B homologue (Taneja et al J.Biol.Chem., (2002), 277,40853-40861); Nucleotide sequence (NM 033647) and corresponding protein sequence are shown in SEQ ID No.1 and SEQ ID No.2.Report proves during G1, need helicase activity to promote that G1/S changes.Gu et al (Mol.Biol.Cell., (2004), 15,3320-3332) show, be called that the little C-terminal district of the helicase 1 B gene of phosphorylation dependency subcellular localization domain (PSLD) is by Cdk2/ cyclin E phosphorylation, containing NLS and NES sequence.Gu et al (Mol.Biol.Cell., (2004), 15,3320-3332) be studied on the cell of the plasmid transient transfection with the coding EGFP-β Gal-PSLD fusions of being expressed by CMV promoter (comprise beta-galactosidase enzymes (β Gal) at construct and be similar to helicase B as inertia group in size to make whole fusion rotein).The cell being in G1 mainly shows EGFP signal in nucleus, and the cell being in other cell cycle phase main showed cell matter EGFP signal.These investigators conclude, before and after the G1/S phase of cell cycle changes, PSLD has guided reporter molecule from nucleus to cytoplasmic transposition.

Suitably, live cell reporter molecule is selected from and comprises fluorescin, enzyme and antigenic markers.Preferably, fluorescin is derived from other member (Labas et al., Proc.Natl.Acad.Sci, (2002), 99,4256-4261) of Aequoria Victoria, Renilla reniformis or Hydrozoa and Anthozoa.More preferably, fluorescin is EGFP (BD Clontech), Emerald (Tsien, Annu.Revs.Biochem., (1998), 67,509-544) or J-Red (Evrogen).Most preferably, fluorescin is selected from green fluorescent protein (EGFP), Emerald and J-Red of green fluorescent protein (GFP), enhancing.

Preferably, reporter molecule is the enzyme reporter molecule of such as halo-tag (Promega).

Suitably, reporter molecule is EGFP or J-Red, and cell cycle phase dependency positioning controling element is PSLD.

Suitably, reporter molecule is series connection (being namely rendered as tandem sequence repeats).

Polypeptide construct, comprises the aminoacid sequence of SEQ ID No.5.

According to a second aspect of the invention, the nucleic acid construct of any polypeptide construct described above of encoding is provided.

Suitably, described nucleic acid construct additionally comprises and is operably connected to and is controlled by least one cell cycle independent expression controlling elements.

Term " is operably connected " and represents that the arrangement of element makes they and its expection object as one man work, and such as initiation transcription in promotor, carries out the DNA sequence dna by code book invention reporter molecule.

Suitably, express controlling elements and control to transcribe in the long-term time period, the transcriptional level of whole cell cycle is limited variable.Preferably, expressing controlling elements is ubiquitin C or CMVI/E promotor, and it provides transcribing for a long time required for stable cell lines production.

Preferably, nucleic acid construct comprises the sequence of ubiquitin C promotor and coding PSLD and EGFP or J-Red.

Optionally, nucleic acid construct comprises the sequence of CMV promoter and coding PSLD and EGFP or J-Red.

In a third aspect of the present invention, provide the carrier comprising nucleic acid construct arbitrarily as mentioned above.Suitably, described carrier is virus vector or plasmid.Suitably, described virus vector is adenovirus carrier or lentiviral vectors.

Optionally, carrier is additionally included in eukaryotic cell the drug resistance gene having function, in mammalian cell, preferably have the drug resistance gene of function.

Expression vector also can comprise other nucleotide sequence, such as poly-adenosine signal, donor splicing site/acceptor splicing site signal, intervening sequence, transcription enhancer sequences, translational enhancer sequence etc.Optionally, drug resistance gene and reporter gene are by internal ribosome entry site (IRES) (Jang et al., J.Virology, (1988), 62,2636-2643) be operably connected, instead of by the promoters driven of separating two genes.PIRES-neo and the pIRES carrier obtained from Clontech business can be used.

A fourth aspect of the present invention, provides the host cell with nucleic acid construct transfection as above.The host cell introducing this construct or the expression vector containing this construct wherein can be any mammalian cell can expressing this construct.

The technology of well known to a person skilled in the art can be used by the DNA transfection of reporter constructs for preparing in host cell.These technology can comprise: electroporation (Tur-Kaspa et al, Mol.Cell Biol. (1986), 6,716-718), based on method (such as Graham and the Van der Eb of calcium phosphate, Virology, (1973), 52,456-467), direct microinjection, method based on cation lipid (such as uses Superfect (Qiagen) or Fugene6 (Roche) and uses transgenosis (the Jiao et al of bombardment mediation, Biotechnology, (1993) 11, 497-502).Utilize the native abilities of virus to enter cell for more alternative approach DNA construct be transfected in cell.The carrier that these methods comprise and transfection procedure are based on such as hsv (United States Patent (USP) 5288641), cytomegalovirus (Miller, Curr.Top.Microbiol.Immunol., (1992), 158,1), vaccinia virus (Baichwal and Sugden, 1986, in Gene Transfer, ed.R.Kucherlapati, New York, PlenumPress, and adenovirus and adeno associated virus (Muzyczka, Curr.Top.Microbiol.Immunol., (1992) p117-148), 158,97-129).

The example of suitable recombinant host cell comprises the mammal cell line of HeLa cell, Vera cell, Chinese hamster ovary cell (CHO), U2OS, COS, BHK, HepG2, NIH 3T3MDCK, RIN, HEK293 and other vitro culture.Preferred host cell is people's cell.These clones can available from American type culture collection (ATCC), Bethesda, Maryland, U.S.A.The present invention also expects the cell comprised from primary cell line, and these clones for some time that after taking off cell from Mammals, then culturing cell is limited is established.

In preferred embodiments, clone is the stable cell lines of the multiple host cell comprised according to fourth aspect.

Also standard method can be used to be used for the clone of the stably express showing cell cycle position reporter molecule to set up in host animal the heterograft (Krasagakis of through engineering approaches cell, KJ et al, Cell Physiol., (2001), 187 (3), 386-91; Paris, S.et al, Clin.Exp.Metastasis, (1999), 17 (10), 817-22).Through engineering approaches is carried out study tumor cell division with the tumor cell line heterograft of express cell period position reporter molecule by making it possible to Modling model system, is stagnated and shift and screen new cancer therapy drug.

In a fifth aspect of the present invention, provide the purposes of polypeptide as above, for determining the cell cycle position of mammalian cell.

The engineered cell lines of express cell period position reporter molecule or genetically modified organism are used as the allograft in host animal, this can study mechanism (the Pye & Watt of tolerance or the repulsion affecting tissue transplantation, J.Anat., (2001), 198 (Pt 2), 163-73; Brod, S.A.et al, Transplantation (2000), 69 (10), 2162-6).

According to a sixth aspect of the invention, provide the method for the cell cycle position for determining mammalian cell, described method comprises:

A) at cells nucleic acid construct as above; And

B) signal launched by monitoring reporter molecule determines cell cycle position.

For carrying out the method for the cell cycle position for determining cell according to the 6th aspect, transfection can be cultivated for some time under certain condition at the cell of DNA reporter constructs, it is enough to make the specified phase in the cell cycle express reporter molecule.Typically, the expression of reporter molecule will occur 16 to 72 hours after transfection, but may change according to culture condition.If reporter molecule is based on green fluorescent protein sequence, reporter molecule may need the time determined to be folded into the conformation of fluorescence.This time depends on the primary sequence of used green fluorescent protein derivative.Fluorescent reporters also can change color (see such as Terskikh, Science, (2000), 290,1585-8) in time, and this needs after transfection with specific timed interval imaging.

If reporter molecule creates the fluorescent signal in the method for the 6th aspect, just can use the fluorescent microscope of routine or monitor the signal of transmitting based on confocal fluorescent microscope.Use these technology, just can determine the cell proportion of expressing reporter molecule, and the location of reporter molecule.In the method according to the invention, can to measure in the cell sorter of such as spectrophotometer, photofluorometer, fluorescent microscope, cooled charge coupled device (CCD) imager (such as scanning imaging instrument or area imaging instrument), fluorescence excitation, confocal microscope or scanning confocal equipment suitably by optical instrument and transform or the fluorescence of cell of transfection DNA construct, wherein excite by scan light and launch the spectral quality determining cell in substratum.

In embodiments of the invention, nucleic acid reporter construct wherein comprises drug resistance gene, in transfection with after expressing drug resistance gene (usual 1-2 days), by the cell of the reporter gene of expressing modified is selected in cell growth under microbiotic exists, the existence due to selection marker thing gene makes the Cells with Antibiotics of transfection have resistance.Adding antibiotic object is the cell of selecting to express reporter gene, and the promotor that reporter gene associates together with it is incorporated in the genome of clone by these cells in some cases.After selection, standard technique can be used to be separated the cloned cell line of expression construct.Then cloned cell line can grow at the standard conditions, and the specified point in the cell cycle is expressed reporter molecule and produced detectable signal.

Can cultivate the cell of transfection according to nucleic acid reporter construct of the present invention when lacking and/or there is test agent to be studied, the impact of the cell cycle of described agents on cellular is to be determined.By determining to express the location of the signal in the ratio of cell of reporter molecule and cell, determine that the impact of test agent on the cell cycle of cell is possible, such as whether test macro makes cells arrest in the specified phase of cell cycle, or impact accelerates or deceleration cell fission.

Therefore, according to a seventh aspect of the invention, provide and determine the method for test agent on the impact of the cell cycle position of mammalian cell, the method comprises:

A) nucleic acid reporter construct as above is expressed when lacking and there is test agent; And

B) determine cell cycle position by the signal launched of monitoring reporter molecule, wherein when lacking and there is test agent measured transmit between difference indicate the impact of test agent on the cell cycle position of cell.

Term " test agent " should be understood to form or the chemical entities of electromagnetic radiation.Preferably, test agent is the chemical entities being selected from medicine, nucleic acid, hormone, protein and peptide.Test agent can be that exogenous application arrives cell, or can be carrying out peptide expressed in the cell studied or protein.

In a eighth aspect of the present invention, provide and determine the method for test agent on the impact of the cell cycle position of mammalian cell, the method comprises:

A) when there is described test agent at described cells nucleic acid reporter construct as above;

B) signal launched by monitoring reporter molecule determines cell cycle position, and

C) given value of signal and the signal launched when lacking test agent launched when there is test agent is compared;

Difference wherein when there is test agent between measured given value when transmitting and lack test agent indicates the impact of test agent on the cell cycle position of cell.

In a ninth aspect of the present invention, provide and determine the method for test agent on the impact of the Cell cycle status of mammalian cell, the method comprises:

A) cell containing nucleic acid reporter construct described above is provided;

B) exist and lack test agent and cultivate the first and second cell masses under allowing the condition of express nucleic acid reporter constructs respectively; And

C) signal that the reporter molecule in the first and second cell masses is launched is measured;

Wherein in the first and second cell masses measured transmit between difference indicate the impact of test agent on the cell cycle position of cell.

According to the tenth aspect of the invention, provide and determine the method for mammalian cell cycle on the impact of cell processes, what described process changed in test agent by known response first can detect reporter molecule to measure, and the method comprises:

A) when there is test agent at cells the second nucleic acid reporter construct as above;

B) signal launched by monitoring the second reporter molecule determines cell cycle position; And

C) monitoring first can detect the signal that reporter molecule is launched,

Wherein step b) whether be cell cycle dependant to determined cell cycle position and first relation that can detect between signal that reporter molecule launches if indicating described cell processes.

In a eleventh aspect of the present invention, provide polypeptide as above for measuring the purposes of the CDK2 activity in cell.

According to a twelfth aspect of the invention, provide the method measuring CDK2 activity in cell, said method comprising the steps of

A) at cells nucleic acid construct as above, and

B) signal launched by monitoring reporter molecule determines that CDK2 is active.

According to a thirteenth aspect of the invention, provide for determining the method for test agent on the impact of the CDK2 activity of mammalian cell, described method comprises:

A) when lacking and there is described test agent at described cells nucleic acid construct as above; And

B) determine that CDK2 is active by the signal launched of monitoring reporter molecule, wherein when lacking and there is described test agent measured transmit between difference indicate the impact of test agent on CDK2 activity.

In a fourteenth aspect of the present invention, provide and determine the method for test agent on the impact of mammalian cell CDK2 activity, described method comprises:

A) when there is described test agent at described cells nucleic acid construct as above; And

B) signal launched by monitoring reporter molecule determines cell cycle position,

C) signal launched when there is test agent and the given value transmitted when lacking test agent is compared;

Difference when wherein there is test agent between measured described given value when transmitting and lack test agent indicates the impact of test agent on cell CDK2 activity.

Accompanying drawing is sketched

The present invention is set forth further with reference to the following examples and accompanying drawing, wherein:

The location of Fig. 1-HDHB in nucleus or tenuigenin.

(A) analyze tenuigenin and the nucleus extraction thing of U2OS cell, it is by denaturing gel electrophoresis and the western trace that carries out with the antibody of anti-restructuring HDHB, alpha-tubulin and PCNA.By chemiluminescence detection immunoreactive protein.

(B) HDHB of the GFP mark of also transient expression in microinjection to U2OS cell is observed by fluorescent microscopy.With Hoechst dyeing nucleus.Proportional sizes, 10 μm.

(C) HDHB of the FLAG mark of also transient expression in microinjection to U2OS cell is observed by fluorescent microscopy.

The Subcellular Localization of Fig. 2-GFP-HDHB is cell cycle dependant.

(A) Subcellular Localization of the HDHB of the GFP mark of transient expression in quantitatively asynchronous, G1 and S phase U2OS cell.The quantity of the GFP positive cell with specific distribution pattern is expressed as the per-cent of GFP positive cell sum (>100 cell).

(B) analyze synchronous U2OS cell (G1 and S phase) tenuigenin and nucleus extraction thing, it is by denaturing gel electrophoresis and the western trace that carries out of antibody with anti-restructuring HDHB, alpha-tubulin and PCNA.By chemiluminescence detection immunoreactive protein.

The confirmation in Fig. 3-HDHB apoptotic nueleolus desired structure territory.

(A) schematic diagram of HDHB protein, shows seven potential CDK phosphorylation sites (SP or TP), the subcellular localization domain (SLD) of presumption and phosphorylation SLD (PSLD), walker A and walker B helicase motif.Total number of atnino acid is shown below protein.

(B) HDHB of GFP and the FLAG mark produced in research and C-terminal truncated mutant.The C-terminal of HDHB SLD (residue 1040-1087) and PSLD (residue 957-1087) is fused on GFP-β Gal reporter molecule to form GFP-β Gal-SLD and GFP-β Gal-PSLD respectively.

(C) Subcellular Localization of the GFP-HDHB-Δ SLD of transient expression in quantitatively asynchronous, G1 and S phase U2OS cell, and be expressed as the per-cent of GFP positive cell sum.

Fig. 4-GFP-β Gal-PSLD Subcellular Localization pattern is with the change of cell cycle.

(A) GFP-β Gal, the GFP-β Gal-SLD of transient expression and the Subcellular Localization of GFP-β Gal-PSLD in quantitatively asynchronous, G1 and S phase U2OS cell, and be expressed as the per-cent of GFP positive cell sum.

The discriminating of the functional rev type nuclear export signal (NES) in the SLD of Fig. 5-HDHB.

(A) NES (Henderson and Eleftheriou, 2000 of identifying in the presumption NES in HDHB and other cell cycle related proteins matter; Fabbro and Henderson, 2003) comparison.The subscript on aminoacid sequence top represents residue number.Conservative aliphatic residue in thick arrow points NES.The two pairs of residues estimated in HDHB in NES are sported L-Ala, as shown in thin arrow, form Mut1 and Mut2.

(B) with (+) or need not (-) LMB HDHB that transient expression GFP and FLAG marks in the U2OS cell of asynchronous growth with the core suppressing CRM1 and mediate output.The Subcellular Localization of GFP-HDHB and FLAG-HDHB in quantitatively asynchronous, G1 and S phase cell, and be expressed as the per-cent of GFP positive cell sum in this sample.

(C) Subcellular Localization of GFP-HDHB and the GFP-β Gal-PSLD of wild-type and sudden change in quantitative nonsynchronous USOS cell, and be expressed as the per-cent of GFP positive cell sum in this sample.

FLAG-HDHB phosphorylation in Fig. 6-cell cycle dependant body.

(A) use [ ³²p] orthophosphoric acid salt mark transient expression FLAG-HDHB (swimming lane 1) and the U2OS cell of truncated mutant 1-1039 (swimming lane 2) and 1-874 (swimming lane 3) thereof.With anti-FLAG resin immunoprecipitation cell extract.Through the protein of 7.5%SDS-PAGE precipitation separation, on transfer road pvdf membrane, by radioautograph (on) or western trace (under) detect.The position instruction on the left of the protein marker of known molecular amount.

(B) with the FLAG-HDHB of anti-FLAG resin immunoprecipitation U2OS cells, when existence (+) or when lacking (-) inhibitors of phosphatases with (+) or need not (-) λ-Phosphoric acid esterase (λ-PPase) incubation, as indicated, to analyze and with anti-HDHB antibody immunoblotting through SDS-PAGE.

(C) by express FLAG-HDHB U2OS cells arrest G1/S (on) or G2/M (under), then remove blocking-up.At the time point results FLAG-HDHB of instruction, with anti-FLAG resin immunoprecipitation, use (+) or need not process by (-) λ-PPase, analyzing as in (B).

Fig. 7-confirmation S967 is phosphorylation in vivo site main in HDHB.

(A) phosphoamino acid (right side) of FLAG-HDHB of two dimensional separation phosphoamino acid mark (left side) and 32P mark ex vivo, passes through autoradiography observation.Some phospho-peptides be not exclusively hydrolyzed are retained near original place (+).

(B) with radio-labeled wild-type and mutant FLAG-HDHB protein in orthophosphoric acid salt body, immunoprecipitation, is separated through SDS-PAGE, by radioautograph (on) and with anti-HDHB immunoblotting (under) analyze.

(C) phospho-peptide of the wild-type of two dimensional separation 32P mark and the trypsin hydrolyzing of S967A mutant FLAG-HDHB, passes through autoradiography observation.

Fig. 8-confirmation cyclin E/CDK2 is the potential G1/S kinases of HDHB S967.

(A) two dimensional separation carrys out the phospho-peptide of the FLAG-HDHB of phosphorylation in vivo in Fig. 7 C freely or the trypsin hydrolyzing of purified cyclin E/CDK2 or cyclin A/CDK2 phosphorylation in vitro restructuring HDHB, be separated separately or as mixture, pass through autoradiography observation.

(B) by analyze U2OS cells with the antibody immunoblotting of anti-HDHB (swimming lane 1-6), cyclin E (swimming lane 1-3) or cyclin A (swimming lane 4-6) with the protein of FLAG carrier (swimming lane 1,4) or FLAG-HDHB (swimming lane 2,5) co-immunoprecipitation.Parallel analysis (swimming lane 3,6) is carried out as positive control using 1/10th of molten for the cell being used for immunoprecipitation born of the same parents' thing.

The Subcellular Localization of Fig. 9-HDHB is subject to the control of S967 phosphorylation.

(A) Subcellular Localization of GFP-HDHBS967A and S967D of quantitatively asynchronous, G1 and S phase U2OS cells.

In the asynchronous U2OS cell of the stably express of Figure 10-display pCORON1002-EGFP-C1-PSLD carrier, the location of EGFP-PSLD is cell cycle dependant.Fluorescent microscopy is carried out to the same partial visual field of cell, wherein (A) uses Hoechst dyeing nucleus, (B) EGFP-PSLD is observed, (C) nucleus is exposed to BrdU 1 hour, then fix, with the antibody test of Cy-5 mark, to indicate S phase cell.(D) redness (the Cy-5 Immunofluorescence test of BrdU) of the individual cells existed in complete vision and the nucleus fluorescence intensity figure of green (EGFP-PSLD).

Figure 11-pCORON1002-EGFP-C1-PSLD Vector map.

Figure 12-pCORON1002-EGFP-C1-β Gal-PSLD Vector map.

Figure 13-flow cytometry data, compares brightness and the homogeneity of the signal of representative stable cell lines and the parent U2OS clone developed with pCORON1002-EGFP-C1-PSLD, pCORON1002-EGFP-C1-β Gal-PSLD.

Detailed Description Of The Invention

method

plasmid

By using total length HDHB cDNA as BglII/NotI fragment (Taneja et al., J.Biol.Chem., (2002) 277,40853-40861) be inserted into pEGFP-C1 carrier (Clontech, Palo Alto, CA) NotI site form pGFP-HDHB and mutant derivative (see Fig. 4 and 6).PFLAG-HDHB is built by the NotI site HindIII/NotI fragment containing total length HDHB cDNA being inserted into pFlag-CMV2 carrier (Eastman Kodak Co., Rochester, NY).The structure of HDHB-SLD (1-1039) of mark is by with the NruI after residue 1034 encoding sequence and with the NotI cracking mark HDHB plasmid in poly joint, by replacing small segment with the double-end oligonucleotide of the compatible 5 ' end of the flush end of residue 1035 to 1039 of encoding, terminator codon and outstanding NotI.For forming pFLAG-HDHB (1-874), the pFLAG-HDHB DNA digested with Klenow polysaccharase treatment S tuI, to form flat end, is connected in pFLAG-CMV2 carrier.For forming pEGFP-β Gal, by the DNA fragmentation of PCR by p β Gal-control (Clontech) amplification coding intestinal bacteria beta-galactosidase enzymes (β Gal), be inserted into 3 ' end of the GFP encoding sequence in pEGFP-C1, wherein use HindIII site.The HDHB sequence of pcr amplification amino-acid residue 1040-1087 (SLD) and 957-1087 (PSLD), is inserted into the 3 ' end of the β Gal cDNA in pEGFP-β Gal, forms pGFP-β Gal-SLD and pGFP-β Gal-PSLD respectively.In HDHB cDNA, NES mutant and phosphorylation site mutant is formed by site-directed mutagenesis (QuikChange, Stratagene, La JoIIa, CA).

PCORON1002-EGFP-C1-PSLD is built by pcr amplification 390bpPSLD region from DNA construct pGFP-C1-β Gal-PSLD.5 ' NheI and 3 ' SalI restriction enzyme sites is incorporated in PSLD fragment, makes it possible to be subcloned in carrier pCORON1002-EGFP-C1 (GE Healthcare, Amersham, UK).The 6704bp DNA construct pCORON1002-EGFP-C1-PSLD obtained, containing ubiquitin C promotor, bacterium ampicillin resistance gene and Mammals neomycin resistance gene (Figure 11).The nucleotide sequence of carrier is shown in SEQ ID No.3.Standard cloning techniques (Sambrook, J.et al (1989)) is used to form three more forms of this carrier; First replace EGFP gene with J-Red (Evrogen), replace neomycin resistance gene by hygromycin gene, replace ubiquitin C promotor by CMV I/E promotor.

The structure of pCORON1002-EGFP-C1-β Gal-PSLD is by NheI and Xmal restriction enzyme digestion pEGFP-C1-β Gal-PSLD, 4242bp EGFP-β Gal-PSLD fragment is inserted in pCORON1002 carrier (GE Healthcare).9937bp DNA construct pCORON1002-EGFP-C1-β Gal-PSLD (Figure 12) obtained is containing ubiquitin C promotor, bacterium ampicillin resistance gene and Mammals neomycin resistance gene.The nucleotide sequence of carrier is shown in SEQ ID No.4.

The protein of EGFP-PSLD fusion rotein and nucleotide sequence are shown in SEQ ID No.5 and 6.

Confirm all constructs by DNA sequencing and replace the correct DNA sequence dna of mutant.

antibody

For restructuring HDHB (the Bethyl Laboratories of purifying, Montgomery, TX) anti-HDHB antibody is produced, affinity purification (Harlow & Lane, Antibodies:A laboratory manual.Cold Spring Harbor Laboratory) on fixing HDHB.

cell cultures, synchronization, microinjection, electroporation, transfection and stable cell lines are formed

U2OS cell is being supplemented 10% foetal calf serum (FBS) (Atlanta Biologicals, Norcross, GA) Eagle substratum (DMEM) (the Gibco BRLLifetechnologies that Dulbecco improves, Carlsbad, CA) in be trained the individual layer of exponential growth in 37 DEG C.Make the U2OS cells arrest of exponential growth at G1/S by incubation 24h in the DMEM containing 5mM thymidine (Sigma-Aldrich, St.Louis, MO).Interim for cell is discharged into S, sucking-off substratum, adds 10%FBS rinsing cell three times with the DMEM heated, adds incubation in 10%FBS at fresh DMEM.In the DMEM that can reach azoles (Sigma-Aldrich) containing 30ng/ml Lip river by the U2OS cells arrest of exponential growth at G2/M16h.For release cells enters G1, collecting mitotic cell by shaking out gently, adding 10%FBS rinsing three times with DMEM, to be then laid on glass cover-slip for microinjection or to be laid in culture dish for further operation.

Cell cycle synchronization is verified by foregoing flow cytometry (Taneja et al., J.Biol.Chem., (2002) 277,40853-40861).Blocking in the experiment that nucleoprotein exports, cell being cultivated 3h in the DMEM of the leptomycin B (LMB) containing 10ng/ml and 10 μMs of cycloheximides (Calbiochem, San Diego, CA) and synthesizes to stop new albumen.As described in (Herbig et al., 1999) cell be laid on glass cover-slip is carried out microinjection, but injection be plasmid DNA instead of protein.

For electroporation, by the U2OS cell (5x10 of asynchronous growth ⁶) trypsinized, collected by centrifugation, is resuspended to the 20mM HEPES (pH 7.4) of 800 μ l, 0.7mMNa ₂hPO4/NaH ₂pO4,137mM NaCl, 5mM KCl, 6mM glucose, final pH 7.4.Add the DNA of 10 μ g, transfer in 0.4cm electroporation cup (BioRad, Hercules, CA), use Gene Pulser Il equipment (BioRad) to carry out electroporation.By plating cells 1h in tissue culture dishes, use fresh culture rinsing, then cultivate 23h.

Prove with the work of transient transfection cell, due to low transfection efficiency, express the problem that high throughput analysis that is uneven and these data produces, porous flat plate form is very difficult.The impact of therefore screening a large amount of siRNA or agents on cellular cycle just needs the generation of the stable cell lines of homogeneous.Toxic action during overexpression long-term due to HDHB, creates stable cell lines with the PSLD region being connected to reporter molecule.With the J-Red derivative transient transfection U2OS cell of pCORON1002-EGFP-C1-PSLD (Figure 11), pCORON1002-EGFP-C1-β Gal-PSLD (Figure 12) or above-mentioned carrier.In a suitable case, 1mg/ml G418 (Sigma) or Totomycin is used to select to express the stable clone of recombination fusion protein.The primary colonies (each construct about 60) be separated by flow cytometry to confirm the level that sensor is expressed and homogeneity, and in a suitable case, uses aforesaid method exploitation subculture clone.

fluorescent microscopy

For indirect IF staining, with phosphate buffered saline (PBS) (PBS) rinsing cell three times, fix 20min with 3.7% formaldehyde in PBS, in 0.2%Triton X-100, thoroughly change 5min, with the 10%FBS incubation 45min in PBS.Add that 10%FBS detects FLAG-HDHB 2h in room temperature with the anti-FLAG antibody (Sigma-Aldrich) of the 1:100 mouse monoclonal in PBS.After rinsing, the red goat anti-mouse of Texas two anti-(Jackson ImmunoResearch Laboratories, WestGrove, PA) of having puted together with 1:100 in PBS adds that 10%FBS is in incubation at room temperature 1h.After three rinsings, with Hoechst33258 (2 μMs in PBS) Incubate cells 10min.Cover glass is placed on ProLongAntifade (Molecular Probes, Eugene, OR).Openlab 3.0 software (Improvision, Lexington, MA) is used to obtain image in ZeissAxioplan 2 imaging system (Carl Zeiss Inc.) with Hamamatsu digital camera.The cell count of the Subcellular Localization showing often kind of pattern is counted, is expressed as the per-cent of evaluated total cellular score (in each experiment 100 to 150 cells).In at least two independent experiments, qualitative assessment often plants the subcellular proteomics of protein.

For GFP fluorescence, with phosphate buffered saline (PBS) (PBS) rinsing cell three times, fix 20min with 3.7% formaldehyde containing 2 μMs of Hoechst 33258, carry out imaging and assessment as above-mentioned.

Triton X-100 is extracted, with cold cytoskeleton damping fluid (CSK, 10mM HEPES [pH 7.4], 300mM sucrose, 100mM NaCl, 3mM MgCl ₂) rinsing cell twice, extract 5min with the 0.5%TritonX-100 in CSK damping fluid (supplementing 1X proteinase inhibitor) on ice, then fix as mentioned above.

In a suitable case, for high-throughput imaging, dynamic imaging (24hr) in the porous flat plate form of stable cell lines fluorescent microscopy and analysis use high-throughput confocal imaging system (IN Cell Analyzer 1000 or IN Cell Analyzer 3000, GEHealthcare, Amersham, UK) in transfection red fluorescent protein (redFP) derivative of pCORON1002-EGFP-C1-PSLD, pCORON1002-EGFP-C1-β Gal-PSLD or these carriers cell on carry out.Cell cycle phase markers algorithm (GE Health Care) is used to analyze image.

metabolic phosphate marks

With wild-type or mutant FLAGHDHB transient transfection U2OS cell (2.5x10 ⁶).After 24h, by cell incubation 15min in the phosphatic DMEM of removing (Gibco BRL Lifetechnologies), with 32P-H3PO4 (0.35mCi/ml substratum; ICNPharmaceuticals Inc., Costa Mesa, CA) radio-labeled 4h.From extract, the FLAG-HDHB of immunoprecipitation phosphoric acid salt mark, is separated through 7.5%SDS/PAGE, transfers on poly(vinylidene fluoride) (PVDF) film as described below.

cell extraction, immunoprecipitation and westrern trace

24h after transfection, by the culture of the FLAG-HDHB transfection for being undertaken analyzing by immunoprecipitation and immunoblotting at molten born of the same parents' damping fluid (50mM Tris-HCI pH 7.5,10% glycerine, 0.1%NP-40,1mM DTT, 25mM NaF, 100 μ g/ml PMSF, 1 μ g/ml AKOLINE, 1 μ g/ml leupeptin) molten born of the same parents in (0.5ml in each 35mm culture dish, or in each 60mm culture dish or 75cm flask 1ml).Extract is wiped off, at the centrifugal 10min of incubated on ice 5min, 14000g from culture dish.With the anti-FLAG agarose (Sigma) of 10 μ l on turner in 4 DEG C of incubation supernatant samples (0.5 to 1mg protein) 2h.With molten born of the same parents' damping fluid rinsing sepharose 4B three times.The protein transduction of immunoprecipitation is moved on on pvdf membrane, with anti-HDHB peptide serum (1:5000), cyclin E antibody (1:1000), cyclin A antibody (1:1000) (Santa Cruz BiotechnologyInc., Santa Cruz, and chemoluminescence agent (SuperSignal CA), PierceBiotechnology Inc., Rockford, IL) through western engram analysis.

Extract for selecting cell core and cytoplasm protein, the U2OS cell converged by tryptic digestion results 80-90%, uses PBS rinsing.By their resuspensions and at 10mMTris-HCl [pH 7.5], 10mM KCl, 1.5mM MgCl ₂, 0.25M sucrose, 10% glycerine, 75 μ g/ml digitonins, 1mM DTT, 10mM NaF, 1mM Na ₃vO ₄, 100 μ g/ml PMSF, in molten born of the same parents 10min on ice in 1 μ g/ml AKOLINE and 1 μ g/ml leupeptin, at the centrifugal 5min of 1000x g.Supernatant liquor fraction is collected as cytosolic extract.To rinsing be precipitated, be resuspended in high-salt buffer (10mM Tris-HCl [pH7.5], 400mM NaCl, 1mM EDTA, 1mM EGTA, 1mM DTT, 1%NP-40,100 μ g/ml PMSF, 1 μ g/ml peptide presses down enzyme and 1 μ g/ml leupeptin) in, at 4 DEG C of shake 10min.After supersound process, using containing solubility and suspended substance in conjunction with chromatinic protein analyze as nucleus extraction thing.By the protein in 8.5%SDS-PAGE analysis of cells core and Cytoplasmic extract, the antibody of anti alpha tubulin, PCNA (all from Santa Cruz Biotechnology) and restructuring HDHB is then used to carry out wester trace.

protein phosphatase reacts

30 DEG C with 100U in phosphatase buffer (50mM Tris-HCl [pH 7.5], 0.1mM EDTA, λ-Phosphoric acid esterase (New England Biolabs, Beverly, MA) incubation 0.01%NP-40) is attached to the FLAG-HDHB 1h of anti-FLAG pearl.Exist or lacking inhibitors of phosphatases (5mM Na ₃vO ₄, 50mM NaF) and when reacts.By 7.5%SDSPAGE (acrylamide-bisacrylamide ratio, 30:0.36) isolated protein, detect HDHB with anti-HDHB serum and chemoluminescence through western trace.

the peptide mapping of trypsin hydrolyzing and phosphorylated amino acid assay

24h after transfection, as above the culture of the radiolabeled FLAG-HDHB transfection by being used for immunoprecipitation and phosphoamino acid or phospho-peptide mapping is processed, but with RIPA damping fluid (50mM Tris-HCl [pH7.5], 150mM NaCl, 1%NP-40,0.5% Septochol, 1%SDS, 50mM NaF, 1mM EDTA, 5mM Na ₃vO ₄, 100 μ g/mlPMSF, 1 μ g/ml AKOLINE and 1 μ g/ml leupeptin) and replace molten born of the same parents' damping fluid.The protein precipitated through 7.5%SDS-PAGE separating immune is also transferred on pvdf membrane.The film twice containing radiolabeled HDHB is fully rinsed, then through autoradiography observation phosphoprotein with deionized water.Then phosphoprotein is cut, with methyl alcohol then with water again wetting film fragment.With the 50mMNH containing 0.1%Tween 20 (Sigma-Aldrich) ₄hCO ₃at room temperature closing membrane 30min, use 50mM NH ₄hCO ₃rinsing three times, then uses the ox pancreas trypsin Worthington of L-(tolylsulfonyl amido-2-phenyl) ethyl chloride methyl ketone process, Lakewood, NJ) phosphoprotein is scaled off from enzyme PVDF.Then as described in detail in other place, (Boyle et al., Meth.Enzymology, (1991), 201,110-149) carries out two-dimentional phospho-peptide mapping or the phosphorylated amino acid assay of peptide.

cyclin dependant vitro kinase is reacted

Cyclin/CDK (200pmol/h) (being provided by R.Ott and C.Voitenleitner) of purifying and restructuring HDHB (the Taneja et al. of purifying are provided, J.Biol.Chem., (2002) 277,40853-40861) carry out kinase reaction (Voitenleitner et al. as substrate like that according to described in the past, MoI.Cell.Biol., (1999), 19,646-56).

brdU mark, the Chemical cell Cycle block fastened at stabilized cell and RNAi experiment are really recognize

The substratum (100 μ l/ hole) of antibiotic-free is used to express the stabilized cell of pCORON1002-EGFP-C1-PSLD construct with 0.3x10 ⁵/ ml is seeded in 96 hole Greiner flat boards, incubation 16 hours.

For confirming that EGFP-PSLD is in the interim distribution of S, cell amplification test kit (AmershamBiosciences, GE Health Care) BrdU is used to mark stabilized cell 1hr.Cell is fixing in 2% formalin, with second antibody system (the cell amplification test kit of Cy-5 mark; GE Health Care) BrdU that mixed by Immunofluorescence test.With hoechst (2 μMs) staining cell core.

For chemical block research (table 1), stabilized cell is exposed to olomoucine, roscovitine, Luo Keda azoles, mimosine, Omaine or colchicine (Sigma).Cell is fixing in 2% formalin, with hoechst (2 μMs) staining cell core.

SiRNA is studied, by resisting the siRNA of some cyclin, MCM albumen, CDK, polo sample kinases (PLK) set (Dharmacon) and random controls duplex (table 2) to be diluted to 25nM in lipofectamine/optimemI (Invitrogen), join 4hr in stabilized cell.Replace substratum, Incubate plates 48hr.Cell is fixing in 2% formalin, with hoechst (2 μMs) staining cell core.

After IN Cell Analyzer system (GEHC) carries out high-throughput imaging and analyzing, hoescht is used to obtain the data of the nuclear signal intensity (BrdU) under the average core density of the individual cells sum in the visual field and N:C ratio (EGFP signal), core size (hoescht signal) and suitable conditions as nucleus marks thing and IN Cell Analyzer 3000 cell cycle phase markers algorithm (GEHC).Concerning each hole, (mainly core EGFP distributes the total cellular score in each visual field to be categorized into the G1 phase; High EGFP-PSLD nuclear density and N:C than), S phase (3SD on core BrdU signal > background; EGFP-PSLD N:C is than about 1) and G2 phase (larger core size; Lower EGFP-PSLD N:C ratio).Although it is possible for distinguishing M phase cell (according to less core size and very strong EGFP signal), in the hole fixed with formalin, seldom see such cell, because they have been removed in rinsing and fixation procedure.

result

hDHB is present in epipole or tenuigenin

For determining the Subcellular Localization of endogenous HDHB, selective extraction core and cytoplasm protein from people U2OS cell, be separated by denaturing gel electrophoresis, by western engram analysis (Fig. 1).First the existence of PCNA in often kind of extract and alpha-tubulin is monitored to assess extraction procedure.PCNA is enrichment in nuclear extract, and not enrichment in tenuigenin fraction, and alpha-tubulin is mainly found in tenuigenin fraction, confirms fractional separation.HDHB (Fig. 1) is all detected in core and tenuigenin fraction.Tenuigenin HDHB moves slower (Fig. 1) than core fraction, has implied the possibility of posttranslational modification.

These the possibility of result show or HDHB is distributed in whole cell, or mixed cellularity group all contains HDHB in nucleus or tenuigenin.For distinguishing these options, HDHB is located at individual cells situ; By the HDHB that transient transfection marks at people U2OS cells GFP and FLAG.Because mark or the long-term overexpression of unmarked HDHB are Cytotoxic, all experiments are all carried out (usual 24h) in possible most short duration.The mark HDHB analyzed in individual cells by fluorescent microscopy is located.GFP-HDHB and FLAG-HDHB all show two kinds of main station-keeping modes, or in the epipole of disperseing in nucleus, or in tenuigenin (Fig. 1).Also the transient expression of GFP-HDHB in human fibroblasts is all observed in nucleus or tenuigenin.

the confirmation of cell cycle dependant subcellular localization domain in HDHB

With Luo Keda azoles by U2OS cells arrest at G2/M, be discharged into G1 tri-hours, then use in pGFP-HDHB DNA microinjection to their nucleus.After six hours, the expression of GFP-HDHB is easy to detect, and now the G1 phase cell of about 70% is mainly at nucleus accumulation fusion rotein (Fig. 2).On the contrary, when with thymidine by cell synchronization at G1/S time, be discharged into the S phase, then use pGFP-HDHB DNA microinjection, the S phase cell more than 70% is accumulation fusion rotein (Fig. 2) in tenuigenin mainly.The selective extraction display of G1 and S phase U2OS cell, the most of core at G1 of endogenous HDHB and the tenuigenin of S phase (Fig. 2 b).But endogenous HDHB can detect significantly in two kinds of subcellular fractions.Compared with G1 phase albumen, the mobility of S phase HDHB is by slight delay.These results show, the Subcellular Localization of HDHB is regulated in the cell cycle, and the HDHB of GFP mark reflects the location of endogenous unmarked helicase.

Be subject to Bloom Cotard helicase and other RecQ family helicase (Hickson, Nature Rev.Cancer, (2003) 3, the prompting that 169-178), C-terminal nuclear localization signal is differentiated, differentiates the most C-terminal (Fig. 3) at HDHB by possible subcellular localization domain (SLD).For determining whether this SLD estimated is important for HDHB location, prepared C-terminal 48 residues lacked containing SLD HDHB truncated mutant (GFP-HDHB-.SLD) (Fig. 3).By expression vector microinjection in G1 or S phase U2OS cell, after six hours, checked the Subcellular Localization of fusion rotein by fluorescent microscopy.Cell more than 95% have accumulated fusion rotein in tenuigenin, and no matter how (Fig. 3 c) the HDHB cell cycle time of expressing.This result shows that HDHB may with NLS, and it is lacked by C-terminal and weakened or eliminate in GFP-HDHB-Δ SLD.

Whether enough for nuclear location for determining the C-terminal structural domain of HDHB, use bacteria beta-galactosidase (β Gal) as report albumen, because it has the molecular weight (112kDa) close to HDHB, and not containing subcellular localization signal (Kalderon et al., Cell, (1984), 39,499-509).In contrast, preparation GFP-β Gal expression vector (Fig. 3), will monitor the Subcellular Localization of fusion rotein after expression vector microinjection to U2OS cell.As expected, GFP-β Gal main accumulation in tenuigenin (Fig. 4).On the contrary, in the nucleus and tenuigenin of asynchronous or synchronized U2OS cell, all find GFP-β Gal-SLD (Fig. 4), shown that SLD contains NLS, but be inadequate for the nuclear location of report albumen.Infer perhaps contiguous potential CDK phosphorylation site and may have impact on Subcellular Localization (Fig. 3) in the cell cycle, just construct GFP-β Gal-PSLD, wherein C-terminal 131 residues of the HDHB of the SLD containing presumption and potential CDK phosphorylation site bunch are attached to the C-terminal (Fig. 3) of GFP-β Gal.When GFP-β Gal-PSLD plasmid DNA during transient expression, has found GFP-β Gal-PSLD (Fig. 4) in the tenuigenin of the S phase cell in the nucleus of the G1 phase cell more than 90% and more than 70% in asynchronous and synchronous U2OS cell.Compared with the confocal pattern observed the core GFP-HDHB in G1, being uniformly distributed in GFP-β Gal-PSLD and the EGFP-PSLD albumen whole nucleus in G1, is only a small amount of at kernel.Being uniformly distributed of EGFP-PSLD signal or main at cytoplasmic distribution (Figure 10) is shown to highlighting S phase cell (equaling the asynchronous group of about 60%) with the analysis of stable cell lines of the expression pCORON1002-EGFP-C1-PSLD of BrdU mark.S phase cell does not show the main core of the EGFP-PSLD relevant to G1 cell and distributes.Find that some cells demonstrate absolute kernel repulsion (Figure 10) of EGFP-PSLD reporter molecule, but these cells do not mix BrdU.We suppose, the cell definitely removing EGFP-PSLD in showed cell core is G2.The dynamic imaging of the EGFP-PSLD stable cell lines more than 24 hours demonstrates in the G1 core of EGFP-PSLD mainly after mitotic division, before and after indicating G1/S transformation, (after division of cytoplasm about 3.5 hours) are moved to tenuigenin by core rapidly, and further from G1/S until G2 latter stage (about 19 hours) is gradually from nuclear translocation to tenuigenin; Now, before again dividing, there is cell rounding.These observationss seem to confirm that G2 cell demonstrates the absolute tenuigenin distribution of EGFP-PSLD reporter molecule.When compared with U2OS cell or G2M cell cycle phase markers clone (GEHC), the stable cell lines not finding to express EGFP-PSLD fusions affects the overall length (about 24 hours) of cell cycle.In a word, these data show that the Subcellular Localization of HDHB depends on the cell cycle, the C-terminal PSLD structural domain of HDHB plays Main Function in the Subcellular Localization relying on mode Function protein matter with the cell cycle, HDHB in the core of G1, but translocates to tenuigenin in the S phase with possibly during G2 gradually.

the discriminating of functional rev type NES in HDHB

The verified numerous protein shuttled back and forth between nucleus and tenuigenin comprises the NES (Fig. 5) of the prototype NES being similar to HIV rev albumen.Protein containing rev type NES needs to export factor CRM 1 (exporting albumen 1 also referred to as core) and removes conjugated protein and it is transported to tenuigenin (by Weis, Cell, (2003), 112,441-451 commentary) from nucleus.CRM1 activity (Wolffet al., Chem.Biol., (1997), 4,139-147 during Leptomycin B (LMB) specificity suppresses nucleoprotein to export; Kudo et al., Exp.Cell.Res., (1998), 242,540-547).The inspection of the PSLD sequence in HDHB is shown to the rev type NES (LxxxLxxLxL of presumption; Fig. 5).For determining that the tenuigenin location of HDHB is the need of functional NES, by the expression plasmid of GFP-HDHB or FLAG-HDHB DNA when existing or lacking LMB in microinjection to nonsynchronous, G1 and S phase cell.Checked the location of fusion rotein by fluorescent microscopy, and carry out quantitatively.When there is LMB, two kinds of fusion roteins are all accumulated in nucleus, and with cell cycle irrelevant (Fig. 5), the possibility that this and HDHB contain the rev type NES worked by CRM1 is consistent.But also possible that, HDHB may not be the direct transport thing of CRM1, and its output may be mediated indirectly by some other oroteins.For whether the presumption NES in assessment HDHB is functional, Val/Leu and Leu/Leu of NES motif is mutated into L-Ala to produce NES mutant 1 and 2 (Fig. 5) by us.Comprise GFP-HDHB and GFP-β Gal-PSLD transient expression in asynchronous or synchronized U2OS cell of these NES mutant.Two kinds of NES mutein fusion protein are all accumulated in nucleus in more than the cell of 80%, and no matter when they express (Fig. 5) in asynchronous or synchronization cell.This result shows, NES mutation specific weakens the output of GFP-HDHB and GFP-β Gal-PSLD, proves that functional NES is contained in the PSLD region of HDHB.

fLAG-HDHB is phosphorylated with cell cycle dependant manner in vivo.

Potential CDK phosphorylation site bunch (Fig. 3) in the PSLD structural domain of HDHB shows, the phosphorylation of HDHB may regulate its Subcellular Localization in the cell cycle.If like this, the PSLD region of expection HDHB is phosphorylated with cell cycle dependant manner by people.The phosphorylation in PSLD whether is experienced for test HDHB, with the wild-type of FLAG-HDHB and the expression plasmid transient transfection U2OS cell of C-terminal clipped form, use phosphoric acid salt radio-labeled, then by FLAG-HDHB from cell extract immunoprecipitation out, by the protein (Fig. 6) of denaturing gel electrophoresis, immunoblotting and radioautographic analysis immunoprecipitation.The radio-labeled band (Fig. 6 A, swimming lane 1) of FLAG-HDHB is detected at the same position of immunocompetence HDHB band.Also in vivo by strong phosphorylation (swimming lane 2), and the brachymemma FLAG-HDHB (1-874) lacking PSLD is not by remarkable phosphorylation (swimming lane 3) for the brachymemma FLAG-HDHB that lacks SLD.These results show, SLD is not that HDHDB phosphorylation is necessary, and PSLD is required, and shows that phosphorylation site may be positioned at PSLD.

For checking the HDHB time of phosphorylation in the cell cycle, not using radio-labeled to detect phosphorylation will be easily.Because phosphorylation often reduces the electrophoretic mobility of protein in denaturant gel, by the FLAG-HDHB immunoprecipitation of transient expression, before and after processing with λ-Phosphoric acid esterase (λ-Ppase), check its mobility (Fig. 6 B).When not having λ-Ppase to process, in western trace, detect FLAG-HDHB (swimming lane 1) in two migration bands closely, and the FLAG-HDHB of phosphorylation carries out moving (swimming lane 2) with the mobility of the very fast band of two band as single band.When there is λ-Ppase inhibitor in reacting, FLAG-HDHB carries out moving (swimming lane 3) as two bands identical with the protein of vacation process.These data show, the electrophoretic mobility of FLAG-HDHB is phosphorylated and reduces, and this analytical method may be applicable to follow the trail of the phosphorylation of HDHB in the cell cycle.

For determining whether HDHB relies on mode with the cell cycle and be phosphorylated, and by adding in thymidine to substratum, the U2OS cells arrest of transient expression FLAG-HDHB is stuck in G2/M at G1/S or by adding in Luo Keda azoles to substratum.Cell is discharged the different time periods from blocking-up, and from cell extract, immunoprecipitation goes out FLAG-HDHB.

With or without λ-Ppase incubation immunoprecipitate, then by denaturing gel electrophoresis and western engram analysis (Fig. 6 C).Added by λ-Ppase process from the FLAG-HDHB mobility stagnated in the cell of G1/S, show that protein is phosphorylated (Fig. 6 C, upper figure) at G1/S.From G1/S blocks after release, Phosphoric acid esterase process FLAG-HDHB detects similar mobility and changes (upper figure) after at least nine hours, be also (Fig. 6 C, figure below) blocking in the cell of G2/M.But be released in G1 after four and eight hours at cell, FLAG-HDHB is as single band migration, and the impact by Phosphoric acid esterase process is just less (Fig. 6 C, figure below).By 12 hours after discharging from G2/M blocks, when most cells is just entering S phase (data do not show), the mobility of FLAG-HDHB is added by Phosphoric acid esterase process again, has recovered viewed pattern (figure below) in the cell of Luo Keda azoles stagnation.These results show strongly, and the phosphorylation of FLAG-HDHB is cell cycle dependant, from G1/S until maximum phosphorylation during G2/M, and minimum phosphorylation during G1.

serine 967 is the main phosphorylation sites of the HDHB of ectopic expression

For drawing the phosphorylation site collection of illustrative plates in FLAG-HDHB, first we wish to determine which amino-acid residue has been modified.In body, the phosphorylated amino acid assay display phosphoserine of radiolabeled FLAG-HDHB is FLAG-HDHB major phosphate amino acid in vivo (Fig. 7 A).Assuming that in the PSLD of the cell cycle dependant phosphorylation site of HDHB between residue 874 and 1039 (Fig. 3 A), these sites are modified by CDK, phosphoserine is adorned primary amino acid (Fig. 7 A), and so seven potential CDK sites only have four to incite somebody to action still alternatively site.For testing separately these sites each, construct the FLAG-HDHB expression plasmid becoming the sudden change of L-Ala with corresponding Serine.Use orthophosphoric acid salt radio-labeled in vivo by with the cell of these plasmid transient transfections, by FLAG-HDHB immunoprecipitation, undertaken analyzing (Fig. 7 B) by radioautograph and western trace.These results show, FLAG-HDHB and three kind of mutain is similar to phosphorylation comparably, and S967A mutain is only by weak phosphorylation (Fig. 7 B).This result implies, S967 may be the major site of HDHB phosphorylation in vivo.Explain consistent with this, the electrophoretic mobility of FLAG-HDHB after Phosphoric acid esterase process detecting immunoprecipitation with three kinds of mutains changes, but does not detect with S967A albumen.

For confirming that S967 is the main phosphorylation in vivo site of HDHB, the phospho-peptide carrying out trypsin hydrolyzing with wild-type and S967A sudden change FLAG-HDHB is drawn, and they have used orthophosphoric acid salt metabolic ground radio-labeled (Fig. 7 C).The peptide (left figure) of the radiolabeled peptide of a kind of advantage and a kind of weak mark is observed with wild-type protein.In S967A albumen, lack the phospho-peptide of dominant markers, but the peptide (Fig. 7 C, right figure) of weak mark still can be detected.These results provide extra evidence, prove that Serine 967 is the remarkable phosphorylation sites in HDHB body.

cyclin E/CDK2 is as the kinase whose confirmation of HDHB in potential modification G1/S

For in fact can test CDK modify HDHB, as by the HDHB phosphorylation time in the cell cycle and S967 as the major site modified confirmation as suggested in, the cyclin E/CDK2 of purifying with restructuring HDHB and the external incubation of radio-labeled ATP of purifying or cyclin A/CDK2.After kinase reaction, by denaturing gel electrophoresis isolated protein, transfer on pvdf membrane, detected by radioautograph.Result shows, and restructuring HDHB can by the strong phosphorylation of cyclin E/CDK2 and cyclin A/CDK2.Then process radiolabeled HDHB band further, the phospho-peptide for trypsin hydrolyzing is drawn.By the peptide two dimensional separation that often kind digests, or be separated separately, or be separated after mixing with the trypsin hydrolyzing peptide of phosphorylation in vivo FLAG-HDHB, by autoradiography observation (Fig. 8 A).Created and viewed substantially identical pattern in body internal labeling peptide mapping with the HDHB peptide of cyclin A/CDK2 phosphorylation by cyclin E/CDK2, have a major spot and one comparatively speckle (figure A).When mixture is outer and the peptide of body internal labeling when being separated on a kind of tomographic map, their migrations altogether (Fig. 8 A, the right side).These digital proofs are the sames modified in body in FLAG-HDHB by the major phosphate peptide of cyclin E/CDK2 and the external modification of cyclin A/CDK2 in the restructuring HDHB of purifying.

Because the cyclin E activity in people's cell occurred in the late G1 phase, and cyclin A activity be occur with the S phase subsequently together with (Pines, 1999 that occur; Erlandsson et al., 2000), so it is important for attempting distinguishing whether one of these kinases preferentially can modify HDHB.Cyclin subunit often forms the mixture with substrate protein white matter, their target phosphorylations (Endicott et al., 1999; Takeda et al., 2001).For whether test cell Cyclin E protein or cyclin A can associate with HDHB, the protein of immunoprecipitation FLAG-HDHB and association the cell extract of FLAG-HDHB expression vector or empty FLAG carrier in contrast from transfection.By western engram analysis cell extract and immunoprecipitate (Fig. 8 B).Cyclin E obviously and FLAG-HDHB co-precipitation, but cyclin A is not like this (Fig. 8 B, swimming lane 2 and 5), shows that FLAG-HDHB preferentially may interact with cyclin E in vivo.This interaction may be that HDHB is necessary by cyclin E/CDK2 phosphorylation in vivo, this is conceivable, if like this, the phosphorylation that the sudden change preventing it and cyclin E from associating in HDHB will stop cyclin E/CDK2 and carries out.For the possibility (Fig. 7 B, C) of test FLAG-HDHB mutant S967A owing to can not be phosphorylated in vivo in conjunction with cyclin E, the protein of immunoprecipitation FLAG-HDHB-S967A and association from transfected cell extract, is analyzed by western trace.Cyclin E is equally strong with wild-type FLAG-HDHB with the co-precipitation of mutain.

the phosphorylation of Serine 967 is critical for control HDHB locates.

Subcellular Localization and the phosphorylation of the HDHB of above-mentioned data presentation ectopic expression are controlled with cell cycle dependant manner, have maximum phosphorylation from G1/S to G2/M, and this and HDHB period accumulated in tenuigenin is consistent.These results and S967 as the main phosphorylation in vivo site in HDHB confirmation together, show that the phosphorylation of S967 may regulate the Subcellular Localization of HDHB.For testing this idea, by the expression plasmid microinjection of wild-type GFP-HDHB and mutant S967A, S984A, S1005A and S1021A in synchronized U2OS cell.As expected, wild-type GFP-HDHB is accumulated in the epipole of G1 cell, but in the tenuigenin of S phase cell.But regardless of cycle time, GFP-HDHB-S967A is positioned the epipole (Fig. 9) of about 70% fluorocyte.Other three kinds replace mutant or be positioned nucleus, or resemble wild-type GFP-HDHB and be positioned in tenuigenin.In the effort attempting simulation S967 phosphorylation, Serine 967 is mutated into aspartic acid, at nonsynchronous and synchronized U2OS cells GFP-HDHB-S967D, checks the subcellular proteomics of mutein fusion protein.

The cell of expressing about 60% of GFP-HDHB-S967D shows cytoplasmic fluorescence (Fig. 9 A) in nonsynchronous, G1 phase and S phase cell, proves that S967D suddenlys change and simulates the S967 of phosphorylation.The phosphorylation that these data effectively indicate Serine 967 is critical in the Subcellular Localization regulating HDHB.

the C-terminal structural domain of HDHB imparts cell cycle dependant location

The structural domain PSLD of 131 residues enough by HDHB, EGFP or β Gal reporter molecule with cell cycle dependant manner targeted cells core or tenuigenin (Fig. 4 and 10).Rev-type NES is arranged in this structural domain (Fig. 5), but its activity or to core export mechanism accessibility depend on the PSLD mainly phosphorylation of Serine 967 when G1/S changes (Fig. 6-9).S967 Perfect Matchings is in total CDK substrate identification motif (S/T) PX (K/R).Cyclin E/CDK2 and cyclin A/CDK2 both can external modification HDHB, but the ability of the HDHB compound in cyclin E/CDK2 and cell extract shows that it may be the initial kinases (Fig. 8) modifying HDHB when G1/S changes.For EGFP-PSLD stable cell lines, the adding of known Cdk2 inhibitor olomoucine and roscovitin (table 1) or mainly distribute at core for result in EGFP-PSLD the adding of siRNA (table 2) of cyclin E and be stuck in G1, this supports the possibility that Cdk2/ cyclin E is responsible for controlling the viewed phosphorylation dependency Subcellular Localization based on the cell cycle further.The phosphorylation of PSLD appears to last till the comparatively rear section of cell cycle, this and HDHB in S and G2 mainly tenuigenin locate very relevant.Show with the kinetics image of olomoucine process stable cell lines more than 24 hours, for the cell being stuck in G2, EGFP-PSLD signal redistributed nucleus from tenuigenin and exceedes (not having cell to pass through mitotic division) in about 4-8 hour, this shows when lacking cdk2 activity, EGFP-PSLD otherwise become dephosphorylation and reenter nucleus, to be destroyed and the protein of new synthesis is not phosphorylated because cdk2 suppresses, thus to be arranged in nucleus.

Table 1

Table 2

Can not distinguish whether HDHB experienced by dephosphorylation (Fig. 6 C) or may be targeted when M/G1 changes carry out proteolysis and again synthesize rapidly in early days at G1, it will enter nucleus at that time.But, stable cell lines kinetics imaging more than 24 hours shows, EGFP-PSLD signal during the M phase or M/G1 boundary time not by very large reduction, but within about 30 minutes after division of cytoplasm, just become and mainly suffered (then this state continues about 3 hours during G1) at core, this is formed with nuclear membrane is consistent.This shows that EGFP-PSLD construct is by dephosphorylation, instead of experience is significantly destroyed before and after the M/G1 boundary.

These data provide strong evidence, prove that PSLD contains the active targeting signal (Fig. 2-5,10) not relying on protein environment.Even if due to the sudden change HDHB with inactivation NES when it expresses during the S phase also in core, be exactly so presumably be phosphorylated (Fig. 5), possible NES is not phosphorylated institute's deactivation, CDK control major objective be NES.Extend this reasoning, NES may be masked during G1, and the CDK motif now in PSLD is not modified, and when S967 is phosphorylated, NES has just been released, and causes NES identification (Fig. 3-5) exporting the factor through core.Show to which forms amphipathic alpha-helix to the structural research of rev type NES, leucine is arranged on the side of spiral, and charged residue alignments is at opposite side (Rittinger et al., MoI.Cell.Biol. (1999), 4,153-166).SLD due to HDHB contains rev type NES and NLS, and the alkaline residue that may work as NLS is dispersed in NES, NES and NLS may be positioned at the opposite face of amphipathic helix.Additional sequences in PSLD will shelter NES in molecule, and this makes to only have NLS just can be identified.The phosphorylation of S967 shelters conformation to expose NES by changing in PSLD, and does not affect the exposure of NLS.

the inhibitor of high flux screening cell cycle is carried out with EGFP-PSLD stable cell lines

As mentioned above, understand the difficulty of porous flat plate form with the of service that transient transfection cell carries out, this is due to low transfection efficiency, expresses the problem that high throughput analysis that is uneven and these data produces.Therefore the screening of a large amount of siRNA or agents on cellular cycle influences is just needed to the generation of the stable cell lines of homogeneous.Produce stable cell lines with the PSLD region be connected on reporter molecule (EGFP), described connection is the joint (using pCORON1002-EGFP-C1-PSLD) of the seven amino acid by flexibility.As can be seen from Figure 13, the signal that the fluorescent signal produced with the stable cell lines that pCORON1002-EGFP-C1-β Gal-PSLD sets up produces than the clone of the joint with flexible seven amino acid obviously smaller (about ten times).This may be more need transcribing and translating mechanism of cell due to the size of β Gal albumen.

With pCORON1002-EGFP-C1-PSLD set up stable cell lines (see Figure 13) be homogeneous (average total cell RFU 435, SD 58 in itself; N=271; See Figure 10), provide sensitive, stable with consistent analytical method for the impact (table 1 and 2 of porous flat plate form research cell cycle and screening reagent cell cycle rapidly; Figure 10).

Some aspect disclosed by the present invention is above at Molecular Biology of theCell (15:3320-3332, in July, 2004) in disclose, and on May 14th, 2004 electronics be disclosed in MBC to be delivered, 10.1091/mbc.E04-03-0227, exercise question is " CellCycle-dependent Regulation of a Human DNA Helicase ThatLocalizes in DNA Damage Foci ", these disclosure entirety is introduced here as a reference.

Foregoing is that the present invention is described, is not interpreted as the restriction to it.Although described illustrative embodiments more of the present invention, those skilled in the art will it is readily understood that, the many amendments not deviating from fact new instruction of the present invention and advantage in illustrative embodiment are possible.Therefore, these all amendments are all confirmed as comprising within the scope of the invention, as defined in the claims.So it being understood that foregoing is that the present invention is described, be not interpreted as and be limited to disclosed particular, be all confirmed as comprising within the scope of the appended claims to the amendment of disclosed embodiment and other embodiment.The present invention is limited by claim below, and the equivalent of claim is included in wherein.

SEQ ID NO：1

atggccaggt cgagtccgta cctgcgccaa cttcagggac ctctgctccc acccagggat 60

ctggtggagg aggacgacga ctacctaaac gacgacgtgg aggaggatga agagtccgtg 120

ttcatcgacg ccgaggagct ctgcagtggg ggcgtaaagg ctggcagcct ccccgggtgc 180

ctccgcgttt ctatttgtga tgaaaacaca caagagacat gtaaagtgtt tggacgtttt 240

ccgataacag gtgcttggtg gagagtgaag gtacaagtaa agcctgtggt gggatcaagg 300

agctatcaat atcaagttca aggatttccg tcttactttt tgcagtctga tatgtcacca 360

ccaaatcaaa aacatatctg tgctctcttt cttaaagagt gtgaggtctc cagtgatgat 420

gttaataaat ttttaacatg ggtaaaggag gtatcaaact acaaaaacct aaactttgaa 480

aatcttaggg aaacactaag aactttccac aaggaaactg gaaggaaaga tcaaaagcag 540

cctacacaga atggtcagga agagttgttc ctagacaatg agatgagtct tcctctggaa 600

aacacaattc catttagaaa tgtaatgaca gctttgcagt ttccgaagat aatggaattc 660

cttccagttc ttctgcctcg acactttaaa tggatcatag ggtcaggttc taaagagatg 720

ttgaaagaga tagaagagat tttaggtaca catccgtgga aacttggatt tagtaaaata 780

acctacagag agtggaaact cctgcgatgt gaggcaagtt ggatagcatt ttgtcagtgt 840

gagtctcttc tccagctgat gactgatttg gagaagaatg cattaataat gtattccaga 900

ctgaagcaga tatgtagaga agatgggcac acatatgttg aagtgaatga cttaactttg 960

acattgtcaa atcatatgtc atttcatgct gcttcagagt ctctgaagtt tttgaaggat 1020

attggtgtgg tgacatatga gaagtcctgt gtcttccctt atgaccttta ccatgctgaa 1080

agagccatcg ccttttcaat ttgtgacctg atgaagaaac ctccttggca tttatgtgtc 1140

gatgtcgaaa aggtgcttgc ctctattcac accacaaaac ctgagaattc aagcgatgat 1200

gcattgaatg agagcaaacc tgatgaagta agattagaaa atcctgtgga tgttgtggac 1260

acacaggaca atggtgacca tatttggact aatggtgaaa atgaaattaa tgcagaaata 1320

agtgaagttc agctggatca ggatcaggtt gaagttccac tggatcggga tcaggtggct 1380

gctttggaaa tgatttgctc caatcctgtg acagtcataa gtgggaaagg tggatgtggg 1440

aagaccacaa tcgttagccg tctttttaag catatagagc agttggaaga aagagaagta 1500

aaaaaagcct gtgaagattt tgaacaagac cagaatgctt cagaagaatg gattaccttt 1560

actgagcaaa gtcaactaga ggcggacaag gctatagaag ttttgctcac agcacctaca 1620

gggaaagcag ctggcttact aagacagaaa actggtcttc atgcctacac actgtgtcag 1680

gtcaattata gcttctattc atggactcaa acaatgatga ccacaaacaa accatggaaa 1740

ttttcttcgg ttagagttct ggttgtggat gaagggagtt tggtatctgt aggaatcttc 1800

aaatcggtct taaatttatt gtgtgagcac tccaaacttt ctaagcttat tatccttggt 1860

gacattagac agttacccag tattgaacct ggtaacttgc tgaaagatct ttttgagact 1920

cttaagtcaa gaaattgtgc tattgagcta aagacaaacc atagagcaga atctcagctc 1980

attgtggaca atgctacaag aatctcaaga cgccaatttc caaaatttga tgcagaacta 2040

aatatctctg ataatccaac attacccatc tcaattcaag ataagacatt tatttttgtc 2100

aggctcccag aagaggatgc cagttctcag tcatctaaaa ctaatcatca ctcttgttta 2160

tattctgcag ttaaaacttt actacaagaa aataacttac aaaatgcaaa aacatcacaa 2220

tttattgcat ttagaaggca agactgtgat ctaattaatg actgctgctg caaacactac 2280

acaggccacc tcaccaaaga ccatcagagt agacttgttt ttggaattgg tgataaaatt 2340

tgttgtacca ggaatgcata cctctcagac ttactacctg aaaatatctc tggaagtcag 2400

caaaataatg atctagatgc cagtagtgaa gacttttctg gtacgcttcc tgattttgct 2460

aaaaataagc gtgactttga aagtaacgtt cgactgtgca atggagagat atttttcata 2520

acaaatgatg taactgatgt aacttttgga aagagaagat ctttgaccat taataatatg 2580

gctggcctgg aagtaactgt ggattttaag aaactaatga aatattgtcg cataaaacat 2640

gcatgggcaa gaactattca cacttttcag gggtccgagg agcaaacagt tgtctatgtg 2700

gtggggaagg cgggccgcca gcactggcag catgtctaca ccgccgtgac caggggccgc 2760

tgccgagtgt atgtgattgc agaggagtct cagctccgga atgccattat gaaaaacagt 2820

tttcctagaa aaactcgttt gaaacatttc ttgcaaagta agctctcctc tagcggcgca 2880

cctccagcag attttccgtc cccacggaag agctctggag acagtggagg acccagcaca 2940

ccgtcagcat ctccactccc tgtagtcaca gaccacgcca tgacaaatga tgtcacctgg 3000

agcgaggcct cttcgcctga tgagaggaca ctcacctttg ctgaaagatg gcaattatct 3060

tcacctgatg gagtagatac agatgatgat ttaccaaaat cgcgagcatc caaaagaacc 3120

tgtggtgtga atgatgatga aagtccaagc aaaattttta tggtgggaga atctccacaa 3180

gtgtcttcca gacttcagaa tttgagactg aataatttaa ttcccaggca acttttcaag 3240

cccaccgata atcaagaaac ttag 3264

SEQ ID NO：2

 

MARSSPYLRQ LQGPLLPPRD LVEEDDDYLN DDVEEDEESV FIDAEELCSG GVKAGSLPGC 60

LRVSICDENT QETCKVFGRF PITGAWWRVK VQVKPVVGSR SYQYQVQGFP SYFLQSDMSP 120

PNQKHICALF LKECEVSSDD VNKFLTWVKE VSNYKNLNFE NLRETLRTFH KETGRKDQKQ 180

PTQNGQEELF LDNEMSLPLE NTIPFRNVMT ALQFPKIMEF LPVLLPRHFK WIIGSGSKEM 240

LKEIEEILGT HPWKLGFSKI TYREWKLLRC EASWIAFCQC ESLLQLMTDL EKNALIMYSR 300

LKQICREDGH TYVEVNDLTL TLSNHMSFHA ASESLKFLKD IGVVTYEKSC VFPYDLYHAE 360

RAIAFSICDL MKKPPWHLCV DVEKVLASIH TTKPENSSDD ALNESKPDEV RLENPVDVVD 420

TQDNGDHIWT NGENEINAEI SEVQLDQDQV EVPLDRDQVA ALEMICSNPV TVISGKGGCG 480

KTTIVSRLFK HIEQLEEREV KKACEDFEQD QNASEEWITF TEQSQLEADK AIEVLLTAPT 540

GKAAGLLRQK TGLHAYTLCQ VNYSFYSWTQ TMMTTNKPWK FSSVRVLVVD EGSLVSVGIF 600

KSVLNLLCEH SKLSKLIILG DIRQLPSIEP GNLLKDLFET LKSRNCAIEL KTNHRAESQL 660

IVDNATRISR RQFPKFDAEL NISDNPTLPI SIQDKTFIFV RLPEEDASSQ SSKTNHHSCL 720

YSAVKTLLQE NNLQNAKTSQ FIAFRRQDCD LINDCCCKHY TGHLTKDHQS RLVFGIGDKI 780

CCTRNAYLSD LLPENISGSQ QNNDLDASSE DFSGTLPDFA KNKRDFESNV RLCNGEIFFI 840

TNDVTDVTFG KRRSLTINNM AGLEVTVDFK KLMKYCRIKH AWARTIHTFQ GSEEQTVVYV 900

VGKAGRQHWQ HVYTAVTRGR CRVYVIAEES QLRNAIMKNS FPRKTRLKHF LQSKLSSSGA 960

PPADFPSPRK SSGDSGGPST PSASPLPVVT DHAMTNDVTW SEASSPDERT LTFAERWQLS 1020

SPDGVDTDDD LPKSRASKRT CGVNDDESPS KIFMVGESPQ VSSRLQNLRL NNLIPRQLFK 1080

PTDNQET 1087

SEQ ID NO：3

 

ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg 60

ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag 120

cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag 180

gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg 240

aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat 300

gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt 360

cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct 420

ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc 480

caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg 540

cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga 600

ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt 660

cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct 720

gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt 780

tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc 840

gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg 900

cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat 960

cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg 1020

gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg 1080

ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca 1140

atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct 1200

tttttgttag acgaagcttg gtaccgagct cgatatcgcc accatggtga gcaagggcga 1260

ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 1320

caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 1380

gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 1440

ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 1500

gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 1560

ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 1620

gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 1680

caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 1740

caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 1800

cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 1860

cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 1920

cgccgccggg atcactctcg gcatggacga gctgtacaag ggcaatggcg gcaatgctag 1980

cagcggcgca cctccagcag attttccgtc cccacggaag agctctggag acagtggagg 2040

acccagcaca ccgtcagcat ctccactccc tgtagtcaca gaccacgcca tgacaaatga 2100

tgtcacctgg agcgaggcct cttcgcctga tgagaggaca ctcacctttg ctgaaagatg 2160

gcaattatct tcacctgatg gagtagatac agatgatgat ttaccaaaat cgcgagcatc 2220

caaaagaacc tgtggtgtga atgatgatga aagtccaagc aaaattttta tggtgggaga 2280

atctccacaa gtgtcttcca gacttcagaa tttgagactg aataatttaa ttcccaggca 2340

acttttcaag cccaccgata atcaagaaac ttaggtcgac ccgggcggcc gcttcgagca 2400

gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa 2460

tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat 2520

aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg 2580

gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatccgata aggatcgatc 2640

cgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 2700

tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 2760

gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 2820

ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 2880

agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 2940

ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 3000

gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 3060

ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 3120

ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg 3180

tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac 3240

catggcctga aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga 3300

accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 3360

gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 3420

ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 3480

ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 3540

gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 3600

agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt gattcttctg 3660

acacaacagt ctcgaactta aggctagagc caccatgatt gaacaagatg gattgcacgc 3720

aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 3780

cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 3840

caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 3900

gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 3960

ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 4020

tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 4080

tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 4140

agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 4200

actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 4260

cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 4320

tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 4380

tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 4440

cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 4500

gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgatggcc gcaataaaat 4560

atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat 4620

ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg 4680

acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 4740

cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 4800

gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat 4860

aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 4920

ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 4980

aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 5040

tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 5100

agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 5160

cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 5220

taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 5280

tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 5340

tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 5400

cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 5460

gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 5520

cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 5580

actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 5640

ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 5700

tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 5760

tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 5820

acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 5880

ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 5940

ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 6000

ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 6060

gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 6120

ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 6180

aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 6240

gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 6300

gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 6360

aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 6420

cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 6480

tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 6540

ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 6600

atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 6660

cctggccttt tgctggcctt ttgctcacat ggctcgacag atct 6704

SEQ ID NO：4

 

ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg 60

ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag 120

cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag 180

gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg 240

aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat 300

gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt 360

cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct 420

ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc 480

caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg 540

cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga 600

ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt 660

cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct 720

gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt 780

tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc 840

gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg 900

cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat 960

cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg 1020

gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg 1080

ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca 1140

atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct 1200

tttttgttag acgaagcttg gtaccgagct cgatatcgct agcgctaccg gtcgccacca 1260

tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 1320

gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 1380

gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 1440

tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 1500

agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 1560

tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 1620

tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 1680

agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 1740

gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 1800

accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 1860

acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 1920

tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg 1980

gactcagatc tcgagctcaa gcttccatgt cgtttacttt gaccaacaag aacgtgattt 2040

tcgttgccgg tctgggaggc attggtctgg acaccagcaa ggagctgctc aagcgcgatc 2100

ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 2160

cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 2220

cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag 2280

cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct 2340

caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta 2400

cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta 2460

atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact 2520

cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc 2580

cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg 2640

tgctgcgttg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca 2700

ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg 2760

ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg 2820

gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg 2880

ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc 2940

gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc 3000

tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct 3060

gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc 3120

cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg 3180

atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc 3240

gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt 3300

atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg 3360

atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc 3420

gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc 3480

acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag 3540

gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg 3600

aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg 3660

gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg 3720

gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct 3780

gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt 3840

acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct 3900

ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc 3960

agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg 4020

ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag 4080

tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc 4140

cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat 4200

ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg 4260

tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt 4320

gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga 4380

tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac 4440

cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg 4500

aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag 4560

atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa 4620

ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg 4680

ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc 4740

tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg 4800

accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc 4860

cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc 4920

cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga 4980

tggaaaccag ccatcgccat ttgctgcacg gggaagaagg cacatggctg aatatcgacg 5040

gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattac 5100

agctgagatc tagcggcgca cctccagcag attttccgtc cccacggaag agctctggag 5160

acagtggagg acccagcaca ccgtcagcat ctccactccc tgtagtcaca gaccacgcca 5220

tgacaaatga tgtcacctgg agcgaggcct cttcgcctga tgagaggaca ctcacctttg 5280

ctgaaagatg gcaattatct tcacctgatg gagtagatac agatgatgat ttaccaaaat 5340

cgcgagcatc caaaagaacc tgtggtgtga atgatgatga aagtccaagc aaaattttta 5400

tggtgggaga atctccacaa gtgtcttcca gacttcagaa tttgagactg aataatttaa 5460

ttcccaggca acttttcaag cccaccgata atcaagaaac ttagttttat ttcaaattgt 5520

tccgagtaac tatgtttttc tattggagac aaaatgaaca tcgtaacgtc aaagtaccaa 5580

gataagaatt ctgcagtcga cggtaccgcg ggcccgggcg gccgcttcga gcagacatga 5640

taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta 5700

tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 5760

ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt 5820

tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatccg ataaggatcg atccgggctg 5880

gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 5940

cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 6000

gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 6060

ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 6120

cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 6180

agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 6240

aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 6300

gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 6360

aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc 6420

tccttacgca tctgtgcggt atttcacacc gcatacgcgg atctgcgcag caccatggcc 6480

tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct 6540

gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 6600

gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 6660

aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 6720

tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 6780

aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 6840

gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgattctt ctgacacaac 6900

agtctcgaac ttaaggctag agccaccatg attgaacaag atggattgca cgcaggttct 6960

ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 7020

tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 7080

gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc 7140

acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 7200

ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 7260

aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 7320

ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 7380

cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 7440

gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc 7500

tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 7560

ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag 7620

cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 7680

cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg 7740

aaatgaccga ccaagcgacg cccaacctgc catcacgatg gccgcaataa aatatcttta 7800

ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagcgataag gatccgcgta 7860

tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 7920

ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 7980

gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 8040

gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 8100

gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 8160

tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 8220

caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 8280

ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 8340

gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 8400

aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 8460

ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 8520

atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 8580

gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 8640

gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 8700

atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 8760

aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 8820

actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 8880

aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 8940

tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 9000

ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 9060

agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 9120

tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 9180

aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 9240

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 9300

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 9360

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 9420

gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 9480

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 9540

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 9600

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 9660

cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 9720

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 9780

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 9840

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 9900

ttttgctggc cttttgctca catggctcga cagatct 9937

SEQ ID NO：5

 

MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT 60

LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL 120

VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA 180

DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYKG 240

NGGNASSGAP PADFPSPRKS SGDSGGPSTP SASPLPVVTD HAMTNDVTWS EASSPDERTL 300

TFAERWQLSS PDGVDTDDDL PKSRASKRTC GVNDDESPSK IFMVGESPQV SSRLQNLRLN 360

NLIPRQLFKP TDNQET 376

SEQ ID NO：6

 

atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc 720

aatggcggca atgctagcag cggcgcacct ccagcagatt ttccgtcccc acggaagagc 780

tctggagaca gtggaggacc cagcacaccg tcagcatctc cactccctgt agtcacagac 840

cacgccatga caaatgatgt cacctggagc gaggcctctt cgcctgatga gaggacactc 900

acctttgctg aaagatggca attatcttca cctgatggag tagatacaga tgatgattta 960

ccaaaatcgc gagcatccaa aagaacctgt ggtgtgaatg atgatgaaag tccaagcaaa 1020

atttttatgg tgggagaatc tccacaagtg tcttccagac ttcagaattt gagactgaat 1080

aatttaattc ccaggcaact tttcaagccc accgataatc aagaaact 1128

Claims (27)

1. polypeptide construct, it comprises detectable live cell reporter molecule and cell cycle phase dependency positioning controling element, described element is the phosphorylation dependency subcellular localization domain (PSLD) in the C-terminal Special controlling district of helicase B, described element be positioned at G1 and the S phase during change, the transposition of wherein said construct in mammalian cell shows cell cycle position, it is characterized in that described reporter molecule is less than 1000 daltonian peptide groups by molecular weight and is connected on described element.

2. polypeptide construct according to claim 1, wherein said peptide group has and is less than 700 daltonian molecular weight.

3., according to the polypeptide construct of claim 1 or 2, wherein said peptide group is seven peptides.

4. polypeptide construct according to claim 3, wherein said seven peptides are Gycine-Asparagine-Gly-Gly-Asparagine-Alanine-Serines (GNGGNAS).

5., according to the polypeptide construct of claim 1 or 2, wherein reporter molecule is fluorescin.

6. polypeptide construct according to claim 5, green fluorescent protein (EGFP), Emerald and J-Red that wherein said fluorescin is selected from green fluorescent protein (GFP), strengthens.

7., according to the polypeptide construct of claim 1 or 2, wherein reporter molecule is EGFP.

8. polypeptide construct, comprises the aminoacid sequence of SEQ ID No. 5.

9. coding is according to the nucleic acid construct of any polypeptide construct of aforementioned any one claim.

10. the nucleic acid construct of claim 9, wherein said construct additionally comprises and is operably connected to and is controlled by least one cell cycle independent expresses controlling elements.

The nucleic acid construct of 11. claims 10, wherein said expression controlling elements is ubiquitin C promotor or CMV promoter.

12. according to the nucleic acid construct of any one of claim 9 to 11, it comprises the sequence of CMV promoter and coding PSLD and EGFP or J-Red.

13. according to the nucleic acid construct of any one of claim 9 to 11, and it comprises the sequence of ubiquitin C promotor and coding PSLD and EGFP or J-Red.

14. carriers comprising any nucleic acid construct of any one of claim 9 to 13.

15. carriers according to claim 14, wherein said carrier is virus vector or plasmid.

16. with the host cell of the nucleic acid construct according to any one of claim 9 to 13 or the carrier transfection according to any one of claim 14 to 15.

17. stable cell lines comprising host cell according to claim 16.

18. according to the polypeptide construct of any one of claim 1 to 8 for determining the purposes of the cell cycle position of mammalian cell.

19. for the method for the cell cycle position of determining mammalian cell, described method comprises:

A) at cells according to the nucleic acid construct of any one of claim 9 to 13 or the carrier according to any one of claim 14 to 15; And

B) signal launched by monitoring reporter molecule determines cell cycle position.

20. determine the method for test agent on the impact of the cell cycle position of mammalian cell, described method comprises:

A) when lacking and there is described test agent, at described cells according to the nucleic acid construct of any one of claim 9 to 13 or the carrier according to any one of claim 14 to 15; And

B) determine cell cycle position by the signal launched of monitoring reporter molecule, wherein when lacking and there is described test agent measured transmit between difference indicate the impact of test agent on the cell cycle position of cell.

21. determine the method for test agent on the impact of the cell cycle position of mammalian cell, described method comprises:

A) when there is described test agent, at described cells according to the nucleic acid construct of any one of claim 9 to 13 or the carrier according to any one of claim 14 to 15; And

B) signal launched by monitoring reporter molecule determines cell cycle position,

C) signal launched when there is test agent and the given value transmitted when lacking test agent is compared;

Difference wherein when there is test agent between measured given value when transmitting and lack test agent indicates the impact of test agent on the cell cycle position of cell.

22. determine the method for test agent on the impact of the cell cycle position of mammalian cell, described method comprises:

A) cell containing the nucleic acid construct of with good grounds any one of claim 9 to 13 or the carrier according to any one of claim 14 to 15 is provided;

B) exist and lack test agent and under allowing the condition of express nucleic acid reporter constructs, cultivate the first and second cell masses of described cell respectively; And

C) signal that the reporter molecule in described first and second cell masses is launched is measured;

In wherein said first and second cell masses measured transmit between difference indicate the impact of described test agent on the cell cycle position of described cell.

23. determine the method for mammalian cell cycle on the impact of cell processes, and what described process changed in test agent by known response first can detect reporter molecule to measure, and described method comprises:

A) when there is described test agent, at described cells according to the second nucleic acid reporter construct of any one of claim 9 to 13 or the carrier according to any one of claim 14 to 15;

B) signal launched by monitoring the second reporter molecule determines cell cycle position; And

C) monitor described first and can detect the signal that reporter molecule launches,

Wherein step b) whether be cell cycle dependant to determined cell cycle position and first relation that can detect between signal that reporter molecule launches if indicating described cell processes.

24. according to the polypeptide construct of any one of claim 1 to 8 for measuring the purposes of the CDK2 activity in cell.

In 25. mensuration cells, the method for CDK2 activity, said method comprising the steps of

A) at cells according to the nucleic acid construct of any one of claim 9 to 13 or the carrier according to any one of claim 14 to 15, and

B) signal launched by monitoring reporter molecule determines that CDK2 is active.

26. for determining the method for test agent on the impact of the CDK2 activity of mammalian cell, and described method comprises:

A) when lacking and there is described test agent, at described cells according to the nucleic acid construct of any one of claim 9 to 13 or the carrier according to any one of claim 14 to 15; And

B) determine that CDK2 is active by the signal launched of monitoring reporter molecule, wherein when lacking and there is described test agent measured transmit between difference indicate the impact of test agent on CDK2 activity.

27. determine the method for test agent on the impact of mammalian cell CDK2 activity, described method comprises:

A) when there is described test agent, at described cells according to the nucleic acid construct of any one of claim 9 to 13 or the carrier according to any one of claim 14 to 15; And

B) signal launched by monitoring reporter molecule determines cell cycle position,

C) signal launched when there is test agent and the given value transmitted when lacking test agent is compared;

Difference when wherein there is test agent between measured described given value when transmitting and lack test agent indicates the impact of test agent on cell CDK2 activity.