CN101024688A - Natural antibiotic antiseptic polylysine and its preparing method - Google Patents

Abstract

The invention relates to a natural antibiotic antisepsis antistaling agent and the manufacturing method. It uses streptomyces albus as fungus, taking microorganism ferentation method, expanding cultivating, fermenting, filtering, and gaining polylysine. The process includes the following steps: filtering streptomyces albus, taking multi-step expanding cultivating, mixing fermenting cultivating liquid, taking fermenting to streptomyces albusand cultivating liquid, filtering to gain polylysine. The invention has the advantages of strong producing ability for polylysine, easy to control fermenting condition, and high yield.

Description

A kind of natural antibiotic antiseptic polylysine and preparation method thereof

Technical field

The present invention relates to food processing field, especially relate to a kind of natural antibacterial antisepsis antistaling agent and preparation method thereof.

Background technology

The putrid and deteriorated meeting of food causes enormous economic loss, how to prevent that corruption from being the problem that the food scientist pays close attention to the most..At present, anti-corrosion measure commonly used is to add food preservatives, and food preservatives is divided into Chemical Preservative, natural antiseptic agent and composite anti-corrosion agent, and wherein the most widely used is Chemical Preservative. at present.Except that mineral water, pure water, nearly all quality guaranteed period surpasses the beverage (comprising the various beverages that children drink) more than month, and food such as preserved fruit, pickles comprise the fresh-keeping of medicine, health-care food oral liquid, all need add antibiotic antiseptic.Conventional antibiotic antiseptic mainly is chemosynthesis, and as Sodium Benzoate, potassium sorbate etc., the toxicology safety evaluation proves that already the life-time service Sodium Benzoate can produce harm to people's liver, and can be carcinogenic; Potassium sorbate also has certain side effect to human body, and along with the attention of people to health, the application meeting of natural antiseptic agent and composite anti-corrosion agent is more and more widely..Therefore, research and development antibiotic antiseptic natural, that have no side effect replaces chemical antibiotic antiseptic to become inevitable trend.

Summary of the invention

The preparation method who the purpose of this invention is to provide a kind of polylysine, it produces, and the polylysine ability is strong, and it is stable to go down to posterity, and fermentation condition is grasped easily, polylysine productivity height; Another object of the present invention provides a kind of natural biological metabolism product---polylysine, and it is a kind of natural powerful antimicrobial antistaling agent.

The objective of the invention is to be achieved through the following technical solutions: with the streptomyces albus is bacterial classification, and by microbe fermentation method, through enlarged culturing, fermentation, steps such as filtration obtain final biological metabolism product---polylysine.Its concrete steps are:

A. seed selection: with the streptomyces albus is the polylysine bacterial classification;

B. enlarged culturing: under aseptic condition, be carbon source with glucose, be nitrogenous source with the extractum carnis, be mixed with culture dish with inorganic salt, described polylysine bacterial classification is transplanted to carries out enlarged culturing on the culture dish 2～4 times, each the equal controlled temperature 25-30 of enlarged culturing ℃, hold-time 50-75 hour;

C. prepare fermentation culture: Fructus Hordei Germinatus high-quality Fructus Hordei Germinatus is pulverized the back mix making beating with 3～6 times water, the wort that obtains with wort indirect heating to 40 ℃-55 ℃, and is incubated 40-70 minute; Continue indirect heating to 60 ℃-70 ℃, and insulation 15-40 minute; Indirect heating to 75 is ℃-80 ℃ for the third time, and insulation 10-20 minute; Obtain the wort that concentration is 15%-35% after the filtration, it is standby that this wort is diluted to 5%-20%;

D. fermentation: with step c gained fermentation culture, send into and be warming up to 20 ℃-35 ℃ in the fermentor tank, add step b gained polylysine bacterial classification, be incubated 45-75 hour continuously;

E. filter: the gained mixture that will ferment filters, and filter residue discards, and filtrate was left standstill 20-30 hour, gets supernatant liquor and separates polylysine.

Polylysine is the polypeptide with bacteriostasis efficacy that is aggregated into by α-carboxyl and epsilon-amino by 20～35 lysine residues, it has broad-spectrum antibacterial, can suppress gram-positive microorganism, Gram-negative bacteria, fungi and some thermotolerance genus bacillus etc., the safety performance height, solvability in water is extremely strong, Heat stability is good, under neutral and slightly acidic environment condition, stronger biocidal property is arranged, it is a kind of natural powerful antimicrobial antistaling agent, it is not only harmless to HUMAN HEALTH, what have also has a certain nutrient value, is the direction of developing from now on, is the microbiology class food preservatives that has superior antiseptic property and huge business potential in the present natural antiseptic agent.

The present invention has that to produce the polylysine ability strong, and it is stable to go down to posterity, and fermentation condition is grasped easily, characteristics such as polylysine throughput height; Through the polylysine biological antibiotic sanitas that the inventive method makes, it is strong to have an antibacterial ability, good refreshing effect, and the good characteristics of security that have no side effect, its anti-bacterial refreshing power surpasses 5 times of potassium benzoate, 7 times of potassium sorbate.

Embodiment

Below by embodiment, technical scheme of the present invention is described in further detail.

Embodiment: with the streptomyces albus is the polylysine bacterial classification; In the aseptic inoculation case, be carbon source with glucose, be nitrogenous source with the extractum carnis, be mixed with culture dish with inorganic salt, described polylysine bacterial classification to be transplanted to be carried out enlarged culturing on the culture dish, controlled temperature is 27 ℃, 55 hours hold-times; 27 ℃ of temperature, humidity 60%, enlarged culturing is 3 times continuously; In addition high-quality Fructus Hordei Germinatus is pulverized, mixed with water, in water-soluble pot, heat, be incubated 45 minutes to 45 ℃ in 1: 3.5 ratio, be warming up to 65 ℃ again, be incubated 30 minutes, be warming up to 78 ℃ at last, be incubated 15 minutes, obtain the wort of concentration 22% after the filtration, this wort is diluted to 12% standby; Again wort is added fermentor tank, be warmed up to 30 ℃, will join fermentor tank through the bacterial strain of three grades of enlarged culturing then, keep 30 ℃ of fermentations of temperature 50 hours; The mixture that ferments is centrifugal, to remove slag, its filtrate was left standstill 24 hours, got supernatant liquor and separated polylysine.

At last, should be pointed out that above embodiment only is the more representational example of the present invention.Obviously, technical scheme of the present invention is not limited to the foregoing description, and many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.

Claims (1)

1. natural antibiotic antiseptic polylysine and manufacture method thereof is characterized in that comprising following steps:

A. seed selection: with the streptomyces albus is the polylysine bacterial classification;

B. enlarged culturing: under aseptic condition, be carbon source with glucose, be nitrogenous source with the extractum carnis, be mixed with culture dish with inorganic salt, described polylysine bacterial classification is transplanted to carries out enlarged culturing on the culture dish 2 ~ 4 times, each the equal controlled temperature 25-30 of enlarged culturing ℃, hold-time 50-75 hour, keeping humidity simultaneously was 55% ~ 60%;

C. prepare fermentation culture: Fructus Hordei Germinatus high-quality Fructus Hordei Germinatus is pulverized the back mix making beating with 3 ~ 6 times water, the wort that obtains with wort indirect heating to 40 ℃-55 ℃, and is incubated 40-70 minute; Continue indirect heating to 60 ℃-70 ℃, and insulation 15-40 minute; Indirect heating to 75 is ℃-80 ℃ for the third time, and insulation 10-20 minute; Obtain the wort that concentration is 15%-35% after the filtration, it is standby that this wort is diluted to 5%-20%;

D. fermentation: with step c gained fermentation culture, send into and be warming up to 20 ℃-35 ℃ in the fermentor tank, add step b gained polylysine bacterial classification, be incubated 45-75 hour continuously;

E. filter: the gained mixture that will ferment filters, and filter residue discards, and filtrate was left standstill 20-30 hour, gets supernatant liquor and separates polylysine.