CN101024670A - Soluble maize protein, its preparing method and use - Google Patents
Soluble maize protein, its preparing method and use Download PDFInfo
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- CN101024670A CN101024670A CN 200710086634 CN200710086634A CN101024670A CN 101024670 A CN101024670 A CN 101024670A CN 200710086634 CN200710086634 CN 200710086634 CN 200710086634 A CN200710086634 A CN 200710086634A CN 101024670 A CN101024670 A CN 101024670A
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Abstract
The invention discloses a caustic solubility zein, manufacture method and the application. The zein molecule chain has aldehyde group, and the molecular weight is 100-50000. The raw material is convenient to gain, and the producing technology is easy. The distilled residue could be used as forage. It has high social benefits and social benefits.
Description
One, technical field
The present invention relates to a kind of zein and its production and application, more particularly relate to a kind of soluble maize protein and its production and application.
Two, background technology
The present domestic molten zein of alkali that also do not have, external production technology is also mainly to produce pure molten technology.Its general technology extracts with unit alcohol or polyvalent alcohol for being raw material with the Zein powder, as ethanol or Virahol, with freezing or cold water albumen is separated out then.Its technology causes one side solvent usage quantity very big because solvent is an alcohols, has increased production cost; Make that on the other hand the albumen Application Areas that makes is wideless, has certain limitation.
Consuming day by day and the nondegradation of synthetic materials and the serious environmental pollution that causes of China's Nonrenewable resources.Reproducible environment-friendly material is more and more noticeable, and it is nontoxic to environment.Because traditional zein film is pure dissolubility, cost is higher, and the intensity difference of protein film, and fragility is big, and temperature tolerance is poor.
Only this is in wheat and paddy rice in the output of China for corn, and Zein powder is the byproduct of W-Gum processing, is mainly used to be processed into feed at present.
Three, summary of the invention
One of purpose of the present invention provides a kind of soluble maize protein.
Two of purpose of the present invention provides a kind of preparation method of soluble maize protein.
Three of purpose of the present invention provides a kind of application of soluble maize protein.
These and other purpose of the present invention will further embody and set forth by following detailed description and explanation.
Soluble maize protein of the present invention contains aldehyde radical in the zein molecule segment
Its molecular weight is 100-50000, and further its molecular weight is 500-3000.
The preparation method of soluble maize protein of the present invention comprises the steps:
A, (raw material obtains from starch factory with Zein powder, produce as Shijiazhuang City, Hebei Province city Taurus feed factory) to put in solid-to-liquid ratio be 2-5: the 50-60 of 1 ratio ℃ water soaked 2-4 hour, took out the back cold water flush, dash clarify to liquid till, extract, make the solid liquid carrying rate less than 60%;
B, be 2-8 according to liquid-solid ratio: 1 to add concentration be the NaOH aqueous solution of 10-15%, and accent PH is 11-14, under 60-80 ℃, stirred 3-4 hour, rotating speed 500-1500 rev/min, rotating speed centrifuging 2-10 minute of pressing 4000-10000r/min is then got supernatant liquor after 20-50 ℃ of insulation;
C, in supernatant liquor, press 0.01g/l-10g/l and add halfcystine, 30-50 ℃ insulated and stirred 1-3 hour, rotating speed is 100-1000 rev/min;
D, under 4000-10000r/min centrifugal 2-10 minute, cross the leaching supernatant liquor by 20-50 ℃;
E, add glutaraldehyde or add 1g/l-10g/l formaldehyde by 0.01g/l-8g/l in the supernatant liquor that the D step obtains, be incubated 40-60 ℃, stirred 1-3 hour, rotating speed is 500-1500r/min;
F, adding neutral salt cooperate zone electrophoresis, district bandwidth 2-10cm, and voltage gradient: 10-30v/cm, electrophoresis time 20-50min, temperature: 30-50 ℃ separates out protein;
G, cross the post desalination, dry then dry powder albumen (yield is 65%-95%) with glucose gel G-25.
Soluble maize protein of the present invention has biocidal property, oxidation-resistance and biodegradability, and can be applied in the makeup, with other fiber blend or be fit to do industry environmental protection textile materials.
The molten zein of alkali of the present invention, because solvent system routinely, can participate in the blending and pure the spinning of multiple fiber, wherein more representational be mix with cotton oar Mierocrystalline cellulose, wood pulp fiber element and bamboo pulp fiber element, grafting, prepare the long filament and the staple fibre of all size by the wet spinning technology spinning.Can improve the bad performance of original fiber.Keep pure and fresh, effectively prevent smell retention clothing, suppress bacterial growth, clean simply degradable behind all product abandonments.The soluble maize protein molecular structure has a similarity with hair, can with wool blend, increase feel.Simultaneously, macromolecular chain segment has natural kindliness, can then can improve fabric softness with the regenerated fiber blending.
The albumen that utilizes the present invention to make can have been made better physical strength through crosslinked, modification, and temperature tolerance is good, and the mulch film than high water vapor barrier and oxygen-barrier property is arranged.
Method raw material of the present invention is easy to get, and production technique is simple, and the residue after the extraction can reuse.And the used soluble maize protein of the present invention is that molecular weight is controlled, zein purity height, widened the range of application of zein, and it is big to have solved the traditional technology solvent load, spinning property difference and preparation shortcomings such as fragility is big during protein film with other fiber blends the time.And this soluble maize protein produces the raw materials used offal that corn processed prepares starch that is, and realized the resource circulation utilization, improved the added value of agricultural byproducts, has very high social benefit and economic benefit.
Below further specify the present invention by specific embodiment, but embodiment only be used for the explanation, can not limit the scope of the invention.
In the present invention, if not refer in particular to, all parts, amount are the weight unit based on gross weight, and all raw materials all can be buied from market.
Four, embodiment
Embodiment 1:
Take by weighing Zein powder 333.3g, wherein contain albumen in the Zein powder and calculate by 60%.Zein powder is put into 50 ℃, soaked 3 hours in the water of 1000ml, take out the back cold water flush, dash, extract solid band liquid 45% to the liquid clarification.By adding concentration at 3: 1 is 15% the NaOH aqueous solution, and transferring PH is 11.Under 60 ℃, stirred rotating speed 700r/min 4 hours.Centrifuging 3000r/min, 10min, 20 ℃ get supernatant liquor.In supernatant liquor, add the halfcystine of 0.05g/l, be incubated 50 ℃, stirred rotating speed 400r/min 2 hours.Centrifuging 4000r/min; 10min, gets supernatant liquor by 20 ℃.Add the glutaraldehyde of 1.7g/l, be incubated 40 ℃, stirred rotating speed 400r/min 2 hours.Add ammonium sulfate, cooperate zone electrophoresis, district bandwidth 4cm, voltage gradient: 12v/cm, electrophoresis time: 30min, temperature separates out protein for 40 ℃.Cross the post desalination with glucose gel G-25.Drying, having obtained molecular weight is 700, yield is 72% dry powder albumen.
Embodiment 2:
Take by weighing Zein powder 333.3g, wherein contain albumen in the Zein powder and calculate by 60%.Zein powder is put into 50 ℃, soaked 2 hours in the water of 1000m, take out the back cold water flush, dash, extract solid band liquid 55% to the liquid clarification.By adding concentration at 3: 1 is 15% the NaOH aqueous solution, and transferring PH is 11.Under 80 ℃, stirred rotating speed 700r/min 4 hours.Centrifuging 3000r/min, 10min, 20 ℃ get supernatant liquor.In supernatant liquor, add the halfcystine of 0.05g/l, be incubated 50 ℃, stirred rotating speed 400r/min 2 hours.Centrifuging 6000r/min; 10min, gets supernatant liquor by 20 ℃.Add the glutaraldehyde of 1.7g/l, be incubated 40 ℃, stirred rotating speed 400r/min 2 hours.Add sal epsom, cooperate zone electrophoresis, district bandwidth 4cm, voltage gradient: 12v/cm, electrophoresis time: 30min, 40 ℃ of temperature are separated out protein.Cross the post desalination with glucose gel G-25.Drying, having obtained molecular weight is 700, yield is 80% dry powder albumen.
Embodiment 3:
Take by weighing Zein powder 333.3g, wherein contain albumen in the Zein powder and calculate by 60%.Zein powder is put into 50 ℃, soaked 3 hours in the water of 1000ml, take out the back cold water flush, dash, extract solid band liquid 40% to the liquid clarification.Adding concentration than 5: 1 by the liquid material is 15% the NaOH aqueous solution, and transferring PH is 14.Under 60 ℃, stirred rotating speed 700r/min 3 hours.Centrifuging 3000r/min, 10min, 20 ℃ get supernatant liquor.In supernatant liquor, add the halfcystine of 0.1g/l, be incubated 50 ℃, stirred rotating speed 400r/min 2 hours.Centrifuging 6000r/min; 10min, gets supernatant liquor by 20 ℃.Add glutaraldehyde 1.7g/l, be incubated 40 ℃, stirred rotating speed 400r/min 2 hours.Add sal epsom, cooperate zone electrophoresis, district bandwidth 4cm, voltage gradient: 12v/cm, electrophoresis time: 30min, 40 ℃ of temperature are separated out protein.Cross the post desalination with glucose gel G-25.Drying, having obtained molecular weight is 1500, yield is 84% dry powder albumen.
Embodiment 4:
Take by weighing Zein powder 333.3g, wherein contain albumen in the Zein powder and calculate by 60%.Zein powder is put into 50 ℃, soaked 3 hours in the water of 1000ml, take out the back cold water flush, dash, extract solid band liquid 42% to the liquid clarification.Adding concentration than 5: 1 by the liquid material is 15% the NaOH aqueous solution, and transferring PH is 11.Under 60 ℃, stirred rotating speed 700r/min 3 hours.Centrifuging 3000r/min, 10min, 20 ℃ get supernatant liquor.In supernatant liquor, add the halfcystine of 0.5g/l, be incubated 50 ℃, stirred rotating speed 400r/min 2 hours.Centrifuging 6000r/min; 10min, gets supernatant liquor by 20 ℃.Add glutaraldehyde 1.7g/l, be incubated 40 ℃, stirred rotating speed 400r/min 2 hours.Add sal epsom, cooperate zone electrophoresis, district bandwidth 4cm, voltage gradient: 12v/cm, electrophoresis time: 30min, 40 ℃ of temperature are separated out protein.Cross the post desalination with glucose gel G-25.Drying, having obtained molecular weight is 3300, yield is 90% dry powder albumen.
Embodiment 5
Take by weighing Zein powder 333.3g, wherein contain albumen in the Zein powder and calculate by 60%.Zein powder is put into 50 ℃, soaked 3 hours in the water of 1000ml, take out the back cold water flush, dash, extract to the liquid clarification.Solid band liquid 50%.Adding concentration than 5: 1 by the liquid material is 15% the NaOH aqueous solution, and transferring PH is 11.Under 60 ℃, stirred rotating speed 700r/min 3 hours.Centrifuging 3000r/min, 10min, 20 ℃ get supernatant liquor.In supernatant liquor, add the halfcystine of 1.5g/l, be incubated 50 ℃, stirred rotating speed 400r/min 2 hours.Centrifuging 6000r/min; 10min, gets supernatant liquor by 20 ℃.Add glutaraldehyde 3g/l, be incubated 40 ℃, stirred rotating speed 400r/min 2 hours.Add sal epsom, cooperate zone electrophoresis, district bandwidth 4cm, voltage gradient: 12v/cm, electrophoresis time: 30min, 40 ℃ of temperature are separated out protein.Cross the post desalination with glucose gel G-25.Drying, having obtained molecular weight is 4000, yield is 85% dry powder albumen.
Embodiment 6
Take by weighing Zein powder 333.3g, wherein contain albumen in the Zein powder and calculate by 60%.Zein powder is put into 50 ℃, soaked 3 hours in the water of 1000ml, take out the back cold water flush, dash, extract solid band liquid 49% to the liquid clarification.Adding concentration than 4: 1 by the liquid material is 15% the NaOH aqueous solution, and transferring PH is 11.Under 60 ℃, stirred rotating speed 700r/min 3 hours.Centrifuging 3000r/min, 10min, 20 ℃ get supernatant liquor.In supernatant liquor, add the halfcystine of 0.5g/l, be incubated 30 ℃, stirred rotating speed 400r/min 2 hours.Centrifuging 6000r/min; 10min, gets supernatant liquor by 20 ℃.Add glutaraldehyde 3g/l, be incubated 60 ℃, stirred rotating speed 400r/min 2 hours.Add sal epsom, cooperate zone electrophoresis, district bandwidth 4cm, voltage gradient: 12v/cm, electrophoresis time: 30min, 40 ℃ of temperature are separated out protein.Cross the post desalination with glucose gel G-25.Drying, having obtained molecular weight is 1500, yield is 90% dry powder albumen.
Embodiment 7
Take by weighing Zein powder 333.3g, wherein contain albumen in the Zein powder and calculate by 60%.Zein powder is put into 50 ℃, soaked 3 hours in the water of 1000ml, take out the back cold water flush, towards clarifying to liquid.Extract solid band liquid 45%.Press the liquid material than the NaOH aqueous solution that adds concentration 15% at 4: 1, transferring PH is 11.Under 60 ℃, stirred rotating speed 700r/min 3 hours.Centrifuging 3000r/min, 10min, 20 ℃ get supernatant liquor.In supernatant liquor, add the halfcystine of 1.5g/l, be incubated 50 ℃, stirred rotating speed 400r/min 2 hours.Centrifuging 6000r/min; 10min, gets supernatant liquor by 20 ℃.Add glutaraldehyde 0.5g/l, be incubated 40 ℃, stirred rotating speed 400r/min 2 hours.Add sal epsom, cooperate zone electrophoresis, district bandwidth 4cm, voltage gradient: 12v/cm, electrophoresis time: 30min, 40 ℃ of temperature are separated out protein.Cross the post desalination with glucose gel G-25.Drying, having obtained molecular weight is 4000, yield is 80% dry powder albumen.
Embodiment 8
Take by weighing Zein powder 333.3g, wherein contain albumen in the Zein powder and calculate by 60%.Zein powder is put into 50 ℃, soaked 3 hours in the water of 1000ml, take out the back cold water flush, towards clarifying to liquid.Extract solid band liquid 43%.Adding concentration than 4: 1 by the liquid material is 15% the NaOH aqueous solution, and transferring PH is 11.Under 70 ℃, stirred rotating speed 700r/min 3 hours.Centrifuging 3000r/min, 10min, 20 ℃ get supernatant liquor.In supernatant liquor, add the halfcystine of 1g/l, be incubated 50 ℃, stirred rotating speed 400r/min 2 hours.Centrifuging 6000r/min; 10min, gets supernatant liquor by 20 ℃.Add glutaraldehyde 2g/l, be incubated 50 ℃, stirred rotating speed 400r/min 2 hours.Add sal epsom, cooperate zone electrophoresis, district bandwidth 4cm, voltage gradient: 12v/cm, electrophoresis time: 30min, 40 ℃ of temperature are separated out protein.Cross the post desalination with glucose gel G-25.Drying, obtaining molecular weight is 9000, yield is 85% dry powder albumen.
Embodiment 9
It is 40% the aqueous solution that the zein of preparation among the embodiment 1 is mixed with the albumen solid content, carry out wet-spinning with cellulose wood pulp cellulose uniform mixing then, wherein the amount of zein stoste is the 30wt% of total amount, adopt one time coagulation forming, precipitation bath removes and contains conventional sodium sulfate 260 grams per liters, zinc sulfate 10.5 grams per liters and sulfuric acid 130 grams per liters.45 ℃ of spinning bath temperature, 70 meters/minute of spinning speeds, degree of draft 20%, 20 ℃ of spinning room temperatures, relative humidity 70% prepares the zein long filament.
Embodiment 10
It is 40% the aqueous solution that the zein of preparation among the embodiment 2 is mixed with the albumen solid content, carry out wet-spinning with cellulose cotton oar Mierocrystalline cellulose uniform mixing then, wherein the amount of zein stoste is the 40wt% of total amount, adopt one time coagulation forming, regulate 55 ℃ of spinning bath temperature, 75 meters/minute of spinning speeds, drafting multiple: 1.9 times of total drafts, spinning pump is to 1.4 times of spinning discs, spinning disc is to 1.0 times of first boundlings, and first is bundled to 1.4 times of second boundlings, 30 ℃ of spinning room temperatures, relative humidity 70% prepares the zein short fiber.
Embodiment 11
It is 40% the aqueous solution that the zein of preparation among the embodiment 3 is mixed with the albumen solid content, carry out wet-spinning with cellulose cotton oar Mierocrystalline cellulose uniform mixing then, wherein the amount of zein stoste is the 50wt% of total amount, with the even curtain coating of liquid in comparatively smooth glass surface, make liquid nature levelling, after 3 hours, treat that solvent evaporates fully after, glass shows that the meeting nature forms thin film, this film is taken off can prepare the zein film.
Claims (10)
1, a kind of soluble maize protein is characterized in that containing aldehyde radical in the zein molecule segment, and its molecular weight is 100-50000.
2, soluble maize protein according to claim 1 is characterized in that its molecular weight is 500-5000.
3, the preparation method of soluble maize protein according to claim 1 and 2 is characterized in that comprising the steps:
A, to put into Zein powder in solid-to-liquid ratio be 2-5: the 50-60 of 1 ratio ℃ water soaked 2-4 hour, took out the back cold water flush, dash clarify to liquid till, extract, make the solid liquid carrying rate less than 60%;
B, be 2-8 according to liquid-solid ratio: 1 to add concentration be the NaOH aqueous solution of 10-15%, and accent PH is 11-14, under 60-80 ℃, stirred 3-4 hour, rotating speed 500-1500 rev/min, rotating speed centrifuging 2-10 minute of pressing 4000-10000r/min is then got supernatant liquor after 20-50 ℃ of insulation;
C, in supernatant liquor, press 0.01g/l-10g/l and add halfcystine, 30-50 ℃ insulated and stirred 1-3 hour, rotating speed is 100-1000 rev/min;
D, under 4000-10000r/min centrifugal 2-10 minute, cross the leaching supernatant liquor by 20-50 ℃;
E, add glutaraldehyde by 0.01g/l-8g/l in the supernatant liquor that the D step obtains, be incubated 40-60 ℃, stirred 1-3 hour, rotating speed is 500-1500r/min;
F, adding neutral salt cooperate zone electrophoresis, district bandwidth 2-10cm, and voltage gradient: 10-30v/cm, electrophoresis time 20-50min, temperature: 30-50 ℃ separates out protein;
G, cross the post desalination, dry then dry powder albumen with glucose gel G-25.
4, the preparation method of soluble maize protein according to claim 3 is characterized in that adding 1g/l-10g/l formaldehyde and replaces glutaraldehyde in the E step.
5, the application of soluble maize protein according to claim 1 and 2 is characterized in that this albumen has biocidal property, oxidation-resistance and biodegradability.
6, the application of soluble maize protein according to claim 5 is characterized in that being used for makeup.
7, the application of soluble maize protein according to claim 5, it is characterized in that can with other fiber blend.
8, the application of soluble maize protein according to claim 5 is characterized in that being fit to do industry environmental protection textile materials.
9, the application of soluble maize protein according to claim 5, it is characterized in that this soluble maize protein can mix with cotton oar Mierocrystalline cellulose, wood pulp fiber element and bamboo pulp fiber element, grafting, prepare the long filament and the staple fibre of all size by the wet spinning technology spinning.
10, the application of soluble maize protein according to claim 5 is characterized in that the mulch film that this soluble maize protein can prepare to be had than high water vapor barrier and oxygen-barrier property.
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| CNB2007100866343A CN100500690C (en) | 2007-03-27 | 2007-03-27 | Soluble maize protein, its preparing method and use |
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| CNB2007100866343A CN100500690C (en) | 2007-03-27 | 2007-03-27 | Soluble maize protein, its preparing method and use |
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| CN101024670A true CN101024670A (en) | 2007-08-29 |
| CN100500690C CN100500690C (en) | 2009-06-17 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101168602B (en) * | 2007-11-29 | 2010-09-08 | 武汉大学 | Corn protein/cellulose mixed solution, and preparation method and use thereof |
| CN104039164A (en) * | 2011-11-14 | 2014-09-10 | 龟田制菓株式会社 | Rice-protein composition and method for manufacturing same |
-
2007
- 2007-03-27 CN CNB2007100866343A patent/CN100500690C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101168602B (en) * | 2007-11-29 | 2010-09-08 | 武汉大学 | Corn protein/cellulose mixed solution, and preparation method and use thereof |
| CN104039164A (en) * | 2011-11-14 | 2014-09-10 | 龟田制菓株式会社 | Rice-protein composition and method for manufacturing same |
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| Publication number | Publication date |
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| CN100500690C (en) | 2009-06-17 |
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