CN101016551A - Method of introducing a plurality of DNA fragments simultaneously into DNA vector - Google Patents
Method of introducing a plurality of DNA fragments simultaneously into DNA vector Download PDFInfo
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- CN101016551A CN101016551A CN 200710019865 CN200710019865A CN101016551A CN 101016551 A CN101016551 A CN 101016551A CN 200710019865 CN200710019865 CN 200710019865 CN 200710019865 A CN200710019865 A CN 200710019865A CN 101016551 A CN101016551 A CN 101016551A
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CN200710019865A CN101016551B (en) | 2007-02-01 | 2007-02-01 | Method of introducing a plurality of DNA fragments simultaneously into DNA vector |
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CN200710019865A CN101016551B (en) | 2007-02-01 | 2007-02-01 | Method of introducing a plurality of DNA fragments simultaneously into DNA vector |
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CN101016551A true CN101016551A (en) | 2007-08-15 |
CN101016551B CN101016551B (en) | 2010-05-19 |
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CN200710019865A Expired - Fee Related CN101016551B (en) | 2007-02-01 | 2007-02-01 | Method of introducing a plurality of DNA fragments simultaneously into DNA vector |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101921800A (en) * | 2010-06-08 | 2010-12-22 | 南京师范大学 | Escherichia coli protein expression vector using trigger factor as fusion tag and construction method and application thereof |
CN102719481A (en) * | 2012-06-14 | 2012-10-10 | 中国科学院微生物研究所 | Method for simultaneously transferring multiple genes into microbial genome |
CN103484492A (en) * | 2013-09-30 | 2014-01-01 | 湖南农业大学 | Method for simultaneously ligating multiple DNA (deoxyribonucleic acid) fragments to same carrier |
CN104531749A (en) * | 2014-12-16 | 2015-04-22 | 同济大学 | One-step method for constructing multi-gene modified carrier for eukaryocyte |
CN105483188A (en) * | 2015-12-21 | 2016-04-13 | 生工生物工程(上海)股份有限公司 | Splicing method of DNA fragment |
CN106636213A (en) * | 2016-12-23 | 2017-05-10 | 西安建筑科技大学 | Method for improving acquisition efficiency of electroporated positive recombinants of escherichia coli |
WO2022166572A1 (en) * | 2021-02-08 | 2022-08-11 | 中国科学院分子植物科学卓越创新中心 | Method and system for continuous cloning of long dna fragment |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
WO2002103010A1 (en) * | 2001-06-14 | 2002-12-27 | Plant Bioscience Limited | Methods and materials for targeted gene disruption in actinomycete bacteria |
US20030134270A1 (en) * | 2002-01-14 | 2003-07-17 | Burgess, Robert M. | Rapid method of selecting cells for gene disruption by homologous recombination |
-
2007
- 2007-02-01 CN CN200710019865A patent/CN101016551B/en not_active Expired - Fee Related
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101921800A (en) * | 2010-06-08 | 2010-12-22 | 南京师范大学 | Escherichia coli protein expression vector using trigger factor as fusion tag and construction method and application thereof |
CN101921800B (en) * | 2010-06-08 | 2012-07-04 | 南京师范大学 | Escherichia coli protein expression vector using trigger factor as fusion tag and construction method and application thereof |
CN102719481A (en) * | 2012-06-14 | 2012-10-10 | 中国科学院微生物研究所 | Method for simultaneously transferring multiple genes into microbial genome |
CN102719481B (en) * | 2012-06-14 | 2014-11-05 | 中国科学院微生物研究所 | Method for simultaneously transferring multiple genes into microbial genome |
CN103484492A (en) * | 2013-09-30 | 2014-01-01 | 湖南农业大学 | Method for simultaneously ligating multiple DNA (deoxyribonucleic acid) fragments to same carrier |
CN104531749A (en) * | 2014-12-16 | 2015-04-22 | 同济大学 | One-step method for constructing multi-gene modified carrier for eukaryocyte |
CN105483188A (en) * | 2015-12-21 | 2016-04-13 | 生工生物工程(上海)股份有限公司 | Splicing method of DNA fragment |
CN105483188B (en) * | 2015-12-21 | 2019-04-23 | 生工生物工程(上海)股份有限公司 | A kind of joining method of DNA fragmentation |
CN106636213A (en) * | 2016-12-23 | 2017-05-10 | 西安建筑科技大学 | Method for improving acquisition efficiency of electroporated positive recombinants of escherichia coli |
WO2022166572A1 (en) * | 2021-02-08 | 2022-08-11 | 中国科学院分子植物科学卓越创新中心 | Method and system for continuous cloning of long dna fragment |
CN114908111A (en) * | 2021-02-08 | 2022-08-16 | 中国科学院分子植物科学卓越创新中心 | Method and system for continuous cloning of long DNA fragments |
CN114908111B (en) * | 2021-02-08 | 2024-02-09 | 中国科学院分子植物科学卓越创新中心 | Method and system for continuous cloning of long DNA fragments |
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CN101016551B (en) | 2010-05-19 |
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