CN100581557C - Chinese medicinal composition, its preparation and use in pharmacy - Google Patents
Chinese medicinal composition, its preparation and use in pharmacy Download PDFInfo
- Publication number
- CN100581557C CN100581557C CN200610096418A CN200610096418A CN100581557C CN 100581557 C CN100581557 C CN 100581557C CN 200610096418 A CN200610096418 A CN 200610096418A CN 200610096418 A CN200610096418 A CN 200610096418A CN 100581557 C CN100581557 C CN 100581557C
- Authority
- CN
- China
- Prior art keywords
- extract
- olibanum
- extraction
- weight
- supercritical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicinal Preparation (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
A Chinese-medicinal composition used to prepare the medicines for relaxing pain and relieving inflammation is prepared from frankincense through supercritical extracting, including the extract with beta-cyclodextrin, reflux extracting of the dregs in alcohol, pulverizing the alcohol extract, and mixing it with said included substance.
Description
Technical field
The invention belongs to field of traditional Chinese medicine pharmacy, relate to a kind of clathrate of Olibanum supercritical extract and the Chinese medicine composition that supercritical extraction medicinal residues ethanol extract is formed, the invention still further relates to the preparation technology and the application of this Chinese medicine composition in pharmacy of this Chinese medicine composition.
Background technology
Olibanum is olive subject plant dungarunga or exsiccant resins such as arbor Boswellia carterii Birdw Boswellia carterii Birdn. Bao Da Boswellia carterii Boswellia bhdw-dagand Birdw and wild Boswellia carterii Boswellia neglecta M Moore, contains compositions such as pinene, limonene, a-boswellic acid.About the medicinal part of Olibanum anti-inflammatory and antalgic, different researcheres have different opinions, a kind ofly think that Olibanum volatile oil has toxic action and zest, easily cause nausea, reaction such as vomiting, should give and remove.And another kind of viewpoint is thought, Olibanum volatile oil is the effective site of analgesic activity, should keep.For the antiinflammatory action of Olibanum, the scholar who has thinks that the Olibanum volatile ingredient is an effective ingredient, and the alcohol extraction position has antiinflammatory action, but does not have antipyretic effect.Other has the scholar to think that Olibanum water extract has antiphlogistic effects preferably, is supercritical extract secondly.
The influence factor of supercritical extraction has fluid ratio, the CO of SFE
2Flow, pressure, time, temperature, comminuted powder granularity etc.Pressure is most important parameter among the SFE.Under the uniform temperature, along with the increase of pressure, fluid density significantly increases, and the dissolubility of solute increases, and extraction efficiency improves.But too high pressure obviously improves production cost, and its extraction yield increases limited.In the SFE process, temperature increases, and has strengthened its diffusivity, makes extract at supercritical CO
2Middle dissolubility increases.Help extraction.But along with the increase of temperature, the dissolubility of impurity also increases, and makes subtractive process complicated, thereby reduces the yield of product.Simultaneous temperature increases, CO
2Fluidic density reduces, and makes to the dissolving power decline of solute, reduces product yield.The extraction time increases, and helps the dissolution equilibrium of effective ingredient in supercritical fluid and the solute, and the time that increases extraction just increases the extraction yield.Owing to behind the extraction certain hour, along with the minimizing of effective ingredient in the solute, increase the extraction time again, the extraction yield increases slowly, and energy consumption increases.And some invalid components also is extracted out more, directly influences the quality of product.
The clathrate technology can make medicine peak time in vivo shorten, promote the medicine absorption in vivo, reaching peak concentration raises, improve bioavailability, and the medicine holdup time is in vivo shortened, and eliminate the half-life shortening, eliminate rapidly, medicine can be detained for a long time when reaching the curative effect of curing the disease in vivo, reduce side effect.Cyclodextrin is the product that starch forms after the cyclodextrin glucosan translocase effect that cultivation obtains with the basophilia bacillus cereus, and common has three kinds, is respectively α, β, gamma-cyclodextrin, wherein commonly used with beta-schardinger dextrin-.The loss of volatile oil increases its stability when adopting the beta-cyclodextrin inclusion compound technology to prevent preparation stored; Cover bad stink, reducing stimulates; Improve drug releasing rate, promote drug absorption, improve curative effect.
The report that does not at present adopt supercritical extraction and clathrate technology that Olibanum is studied.
Summary of the invention
The purpose of this invention is to provide a kind of Chinese medicine composition of forming by the clathrate and the supercritical extraction medicinal residues ethanol extract of Olibanum supercritical extract.
Another object of the present invention provides the preparation technology of this Chinese medicine composition.
A further object of the invention provides the application of this Chinese medicine composition in pharmacy.
The objective of the invention is to realize by following technical measures:
A kind of Chinese medicine composition, this Chinese medicine composition obtains through the following steps:
A. Olibanum supercritical extraction, enclose: Olibanum (system) crushed after being dried is become 40～80 purpose granules, CO
2Supercritical extraction, extracting pressure are 18～34Mpa, and extraction temperature is 35～60 ℃, CO
2Flow velocity be 10～30L/h, extracted the separated and collected extract 0.5～2.5 hour; Extract adds the anhydrous alcohol solution of extract weight 10～30%, get the water dissolution of the beta-schardinger dextrin-of 6～10 times of amounts of extract weight with 1～3 times of beta-schardinger dextrin-weight, adopt polishing to carry out enclose, the enclose time is 20～40 minutes, and vacuum drying promptly gets Olibanum CO
2The clathrate of supercritical extract;
B. alcohol extraction: the medicinal residues of Olibanum (system) supercritical extraction are that the ethanol stirring and refluxing of 50-70% is extracted 1～3 time with the concentration of 6～10 times of amounts of medicinal residues weight, each 1～2 hour, left standstill 12～48 hours, draw supernatant, filter, it is 1.15～1.25 clear paste that filtrate decompression is concentrated into 60 ℃ of relative densities, and vacuum drying promptly gets the ethanol extract of Olibanum supercritical extraction medicinal residues;
C. mix: clathrate and ethanol extract are mixed, promptly get described Chinese medicine composition.
Described Chinese medicine composition, its dosage form are pill, tablet, powder, granule, capsule, soft capsule, drop pill.
The preparation technology of described Chinese medicine composition, this preparation technology comprises the following steps:
A. Olibanum supercritical extraction, enclose: Olibanum (system) crushed after being dried is become 40～80 purpose granules, CO
2Supercritical extraction, extracting pressure are 18～34Mpa, and extraction temperature is 35～60 ℃, CO
2Flow velocity be 10～30L/h, extracted the separated and collected extract 0.5～2.5 hour; Extract adds the anhydrous alcohol solution of extract weight 10～30%, get the water dissolution of the beta-schardinger dextrin-of 6～10 times of amounts of extract weight with 1～3 times of beta-schardinger dextrin-weight, adopt polishing to carry out enclose, the enclose time is 20～40 minutes, and vacuum drying promptly gets Olibanum CO
2The clathrate of supercritical extract;
B. alcohol extraction: the medicinal residues of Olibanum (system) supercritical extraction are that the ethanol stirring and refluxing of 50-70% is extracted 1～3 time with the concentration of 6～10 times of amounts of medicinal residues weight, each 1～2 hour, left standstill 12～48 hours, draw supernatant, filter, it is 1.15～1.25 clear paste that filtrate decompression is concentrated into 60 ℃ of relative densities, and vacuum drying promptly gets the ethanol extract of Olibanum supercritical extraction medicinal residues;
C. mix: clathrate and ethanol extract are mixed, get final product.
The application of described Chinese medicine composition in preparation analgesia, anti-inflammatory drug.
Beneficial effect of the present invention:
The Chinese medicine composition that the clathrate of Olibanum supercritical extract provided by the invention and supercritical extraction medicinal residues ethanol extract are formed has antiinflammatory, analgesic activity preferably.Adopt beta-schardinger dextrin-that the Olibanum oil through supercritical extraction is carried out enclose, liquid-powderization can be improved its stability and covers bad smell, improve the utilization rate of medicine, be convenient to preparation, improve curative effect.The supercritical extraction medicinal residues are carried out alcohol extraction, can further better wherein antiinflammatory, analgesic effective site be extracted.Both are mixed, can better bring into play antiinflammatory, the analgesic activity of Olibanum.
Adopt the more traditional organic solvent extraction method of method of supercritical carbon dioxide extraction very big advantage all to be arranged at aspects such as product quality, yield and environmental conservation.Extraction process provided by the invention has that extraction temperature is low, the time is short, extract composition advantage more completely.The present invention adopts orthogonal experiment that the enclose condition is screened, and selecting clathrate yield, clathrate oil content, oily utilization rate for use is the index screening optimised process, and it is investigated and studies.The present invention adopts orthogonal experiment that the alcohol extraction condition is screened, and adopts to such an extent that the cream amount is the index screening optimised process, and it is investigated and studies.Mixture preparation technology provided by the invention is had simple to operate, effective, be easy to advantages such as commercial application.
Preparation technology's screening of the present invention:
1. Olibanum supercritical extraction process list factor is investigated
The principal element that influences Olibanum supercritical extraction yield is extraction kettle pressure, extraction kettle temperature and extraction time.
1.2. extraction kettle pressure is to the influence of Olibanum oil yield
Setting extraction temperature is 50 ℃, extraction 1.5h, and extracting pressure is respectively 18,22,26,30,34Mpa.The results of analysis of variance shows, between each level of extraction kettle pressure significant difference is arranged, but bigger from the too high operating pressure of the angle of producing to the requirement of equipment, drop into higher, to there being certain influence equipment life, the safety that also is unfavorable for operator, therefore final definite preferred 30Mpa of extracting pressure.
1.3. the extraction kettle temperature is to the influence of Olibanum oil yield
Setting extraction kettle pressure is 30MPa, extraction 1.5h, and extraction temperature result's demonstration, in this scope of experiment, the high more extraction that helps Olibanum oil more of temperature.This is because temperature raises, and the motion of Olibanum oil molecule is accelerated, and its viscosity reduces, and mobile increasing helps its dissolving and diffusion.The results of analysis of variance shows that extraction conditions does not have significant difference under 55 ℃ and 60 ℃, therefore determines 55 ℃ of extraction temperature selections.
1.4. the selection of extraction Best Times
Based on above experimentation, setting extraction kettle pressure is 30MPa, and temperature is 55 ℃, the screening optimum extraction time.After variance analysis extracted 1.5h as can be known, Olibanum was fuel-displaced slowly, extraction 2h and 1.5h and there was no significant difference.For saving energy consumption, increase efficient, determine that the extraction time is 1.5h.Can draw optimum process condition from above data is: extraction kettle pressure 30MPa, 55 ℃ of extraction kettle temperature, extraction time 1.5h.
1.5. supercritical extraction optimised process confirmatory experiment
Carry out 3 batches of demonstration tests separately according to the Olibanum optimum process condition, measurement result, the Olibanum oil yield is followed successively by: 19.81%, 19.23%, 19.63%, RSD=1.52%.The confirmatory experiment result, oily extraction yield all is in higher level, shows the optimization credible result, and this process stabilizing is feasible.
2. Olibanum oil clathrate process research
2.1. the preparation method of clathrate
The principal element that influences polishing enclose Olibanum oil effect have oil and β-CD mass ratio, enclose time, add that water is doubly measured and four factors of pure consumption, this empirical factor and horizontal division such as table 1 are arranged in design.
Table 1 orthogonal experiment factor level table
2.2 enclose result and analysis
The result adopts comprehensive grading to seek optimised process thus, and computational methods are as follows: in two indexs of clathrate, choose the soprano respectively as 100 minutes, all the other by formula calculate: score y
i'=100-peak+y
i, then two indexs are invested different weights.Give oily utilization rate 0.6 respectively, the weight coefficient of oil content 0.4 is an index with each index comprehensive scoring, determines optimised process.By intuitive analysis as can be known, each factor affecting A＞C＞B＞D, The results of analysis of variance shows that A factor, B factor, C factor all have significant difference, horizontally is respectively 2＞3＞1,3＞2＞1,2＞3＞1, D factor there was no significant difference determines that technology is: A
2B
3C
2D
3Each level of B factor is compared analysis, do not have significant difference between 2 levels and 3 levels.Therefore can determine that enclose Olibanum oil optimised process is: A
2B
2C
2D
3, i.e. Olibanum oil: beta-schardinger dextrin-(1: 8), enclose time 30min, to add water 2 times of amounts, pure consumptions be 20% of water.
2.3. demonstration test is to the optimised process A of gained
2B
2C
2D
3, carry out 3 batches of demonstration tests, clathrate oil content meansigma methods is 9.35%, RSD=0.64%, the average oil utilization rate is 84.14%, RSD=0.65% illustrates that this process stabilizing is feasible.
3. alcohol extraction process determines
3.1, the Orthogonal Experiment and Design principal element that influences alcohol extraction process has concentration of alcohol, adds alcohol times amount, reflux extracting time and extraction time.Above-mentioned 4 factors are respectively got 3 levels and are carried out L
9(3
4) orthogonal test, factor level sees Table 2, and selecting dried cream amount for use is that evaluation index is estimated, the screening optimum extraction process.
Table 2 factor water-glass
3.2, variance analysis is index with dried cream amount, range analysis result: B＞A＞D＞C.The factor that promptly influences the alcohol extraction effect is in proper order: solvent is doubly measured＞concentration of alcohol＞extraction time＞extraction time.Optimum process condition is A
2B
2C
2D
2, i.e. 60% ethanol, 8 times of amounts each 1.5 hours, are extracted 2 times.
3.3, confirmatory experiment carries out 3 batches of confirmatory experiments according to optimised process, the result shows that this process stabilizing is feasible.
The pharmacodynamics test of medicine of the present invention:
1 experiment material
1.1 laboratory animal
Kunming mouse (Second Military Medical University, PLA's Experimental Animal Center, male and female half and half); SD rat (Second Military Medical University, PLA's Experimental Animal Center, male and female half and half).
1.2 test apparatus
The 4504 type Zu Disi digital stopwatch (Hong Kong Huaxing foundation company limited) that moves; YSD pharmacology physiology is used instrument (production of Bengbu medical college radio two factories) more; FA1004 type electronic balance (Shanghai Hengping Science Instrument Co., Ltd.); 501 type ultrathermostats (Shanghai City experimental apparatus head factory); Volume determination instrument (self-control of physiology teaching and research room of China Medicine University); T6 ultra-violet and visible spectrophotometer (Beijing Puxi General Instrument Co., Ltd's production).
1.3 trial drug
Sodium carboxymethyl cellulose: Shanghai chemical reagents corporation of Chinese Medicine group, lot number: F20020928
Ibuprofen: Sino-America Tianjin Shike Pharmaceutical Co., Ltd., lot number: 05040122
The mixture of the clathrate of Olibanum supercritical extract and supercritical extraction medicinal residues ethanol extract: make according to embodiment 1, hereinafter to be referred as " Chinese medicine composition of the present invention ".
The foundation of 2 animal models and experimental technique
2.1 animal grouping
Each effect experiment is set at matched group (0.5%CMC-Na), positive drug group (ibuprofen), is subjected to reagent thing group (Chinese medicine composition of the present invention) according to divided into groups by the reagent thing.
2.2 mice acetic acid twisting method analgesic test
Get healthy kunming mice, by the body weight random packet, by mice administration volume 0.5ml/20g, gastric infusion is 6 days continuously, fasting 12 hours is freely drunk water, and is administered once in the 7th day again, after the administration 40min lumbar injection 0.6% acetum 0.20ml/ only, observe and record lumbar injection acetum after the writhing response number of times of animal in the 15min.Represent the index of pain degree with number of times, each experimental group writhing response number of times average and matched group compares and carry out the t check.
2.3 the inhibitory action of mice caused by dimethylbenzene xylene ear swelling test
Get kunming mice, by the body weight random packet, gastric infusion is 6 days continuously, and fasting was spent the night in 12 hours, was administered once in the 7th day again, after 1 hour, evenly was coated with dimethylbenzene 0.05mL on the two sides, front and back of mouse right ear, and left ear is left intact.Each group is taken off cervical vertebra execution mice respectively at causing scorching back 60 minutes, cuts ears and lays round auricle in the ears same area respectively with 7mm diameter card punch, and electronic balance claims quality.Calculate swelling degree and inhibitory rate of intumesce, relatively administration group and matched group difference condition are carried out the t check.Computing formula is:
Swelling degree (mg)=auris dextra tablet quality (mg)-left auricle quality (mg)
Inhibitory rate of intumesce=(matched group swelling degree-administration group swelling degree) ÷ matched group swelling degree * 100%.
3 result of the tests and analysis
3.1 Olibanum acetic acid twisting analgesic test experimental result
Result of the test represents that with writhing response number of times in the 15min t check the results are shown in Table 7 between statistical test employing group.
Table 7 acetic acid twisting analgesic test experimental result
Annotate:
*: P＜0.01; Compare (t check) with matched group.
Experimental result shows that the mouse writhing stoichiometric number of Chinese medicine composition of the present invention and matched group relatively have utmost point significant difference (P＜0.01).Chinese medicine composition Dichlorodiphenyl Acetate of the present invention causes the mouse writhing test and has significant analgesia role.
3.2 Olibanum series xylol causes the inhibitory action test of mice ear
Result of the test shows that with the swelling kilsyth basalt t check the results are shown in Table 8 between statistical test employing group.
Table 8 Chinese medicine composition xylol of the present invention causes the inhibitory action result of the test of mice ear
Annotate:
*: P＜0.01; Compare (t check) with matched group
The result shows that the ear swelling degree of Chinese medicine composition of the present invention, ibuprofen group and matched group relatively have utmost point significant difference (P＜0.01), Chinese medicine composition of the present invention is described, the ibuprofen xylol causes mice ear that significant inhibitory effect is arranged.
The specific embodiment
The invention will be further elaborated by the following examples.
Embodiment 1
A. Olibanum supercritical extraction, enclose: Olibanum (system) crushed after being dried is become 60 purpose fine powders, CO
2Supercritical extraction, extracting pressure are 30Mpa, and extraction temperature is 55 ℃, CO
2Flow velocity be 20L/h, extracted the separated and collected extract 0.5 hour.Extract adds 20% anhydrous alcohol solution of extract weight, gets the water dissolutioies of 8 times of amount beta-schardinger dextrin-s of extract weight with 2 times of amounts of beta-schardinger dextrin-weight, adopts polishing to carry out enclose, and the enclose time is 30 minutes, and vacuum drying promptly gets Olibanum CO
2The clathrate of supercritical extract.
B. alcohol extraction: the medicinal residues of Olibanum (system) supercritical extraction are that 60% ethanol stirring and refluxing is extracted 2 times with the concentration of 8 times of amounts of medicinal residues weight, each 1.5 hours, left standstill 12 hours, draw supernatant, filter, filtrate decompression is concentrated into the clear paste of relative density 1.20 (60 ℃), and vacuum drying promptly gets the ethanol extract of Olibanum supercritical extraction medicinal residues.
C, mixing: the gained clathrate is mixed with ethanol extract, promptly get Chinese medicine composition.
D, get the gained Chinese medicine composition, make tablet according to a conventional method.
Embodiment 2
A. Olibanum supercritical extraction, enclose: Olibanum (system) crushed after being dried is become 80 purpose fine powders, CO
2Supercritical extraction, extracting pressure are 34Mpa, and extraction temperature is 60 ℃, CO
2Flow velocity be 30L/h, extracted the separated and collected extract 2.5 hours.Extract adds the anhydrous alcohol solution of extract weight 30%, gets the water dissolution of the beta-schardinger dextrin-of 10 times of amounts of extract weight with 3 times of amounts of beta-schardinger dextrin-weight, adopts polishing to carry out enclose, and the enclose time is 40 minutes, and vacuum drying promptly gets Olibanum CO
2The clathrate of supercritical extract.
B. alcohol extraction: the medicinal residues of Olibanum (system) supercritical extraction are that 50% ethanol stirring and refluxing is extracted 1 time with the concentration of 6 times of amounts of medicinal residues weight, each 1 hour, left standstill 24 hours, draw supernatant, filter, filtrate decompression is concentrated into the clear paste of relative density 1.15 (60 ℃), and vacuum drying promptly gets the ethanol extract of Olibanum supercritical extraction medicinal residues.
C, mixing: the gained clathrate is mixed with ethanol extract, promptly get Chinese medicine composition.
D, get the gained Chinese medicine composition, make capsule or soft capsule according to a conventional method.
Embodiment 3
A. Olibanum supercritical extraction, enclose: Olibanum (system) crushed after being dried is become 40 purpose fine powders, CO
2Supercritical extraction, extracting pressure are 18Mpa, and extraction temperature is 35 ℃, CO
2Flow velocity be 10L/h, extracted the separated and collected extract 1.5 hours.Extract adds the anhydrous alcohol solution of extract weight 10%, gets the water dissolution of the beta-schardinger dextrin-of 6 times of amounts of extract weight with 1 times of amount of beta-schardinger dextrin-weight, adopts polishing to carry out enclose, and the enclose time is 20 minutes, and vacuum drying promptly gets Olibanum CO
2The clathrate of supercritical extract.
B. alcohol extraction: the medicinal residues of Olibanum (system) supercritical extraction are that 70% ethanol stirring and refluxing is extracted 3 times with the concentration of 10 times of amounts of medicinal residues weight, each 2 hours, left standstill 36 hours, draw supernatant, filter, filtrate decompression is concentrated into the clear paste of relative density 1.25 (60 ℃), and vacuum drying promptly gets the ethanol extract of Olibanum supercritical extraction medicinal residues.
C, mixing: the gained clathrate is mixed with ethanol extract, promptly get Chinese medicine composition.
D, get the gained Chinese medicine composition, make granule or powder according to a conventional method.
Embodiment 4
A. Olibanum supercritical extraction, enclose: Olibanum (system) crushed after being dried is become 60 purpose fine powders, CO
2Supercritical extraction, extracting pressure are 26Mpa, and extraction temperature is 45 ℃, CO
2Flow velocity be 20L/h, extracted the separated and collected extract 2 hours.Extract adds the anhydrous alcohol solution of extract weight 20%, gets the water dissolution of the beta-schardinger dextrin-of 8 times of amounts of extract weight with 2.5 times of amounts of beta-schardinger dextrin-weight, adopts polishing to carry out enclose, and the enclose time is 30 minutes, and vacuum drying promptly gets Olibanum CO
2The clathrate of supercritical extract.
B. alcohol extraction: the medicinal residues of Olibanum (system) supercritical extraction are that 60% ethanol stirring and refluxing is extracted 3 times with the concentration of 6 times of amounts of medicinal residues weight, each 1.5 hours, left standstill 48 hours, draw supernatant, filter, filtrate decompression is concentrated into the clear paste of relative density 1.15 (60 ℃), and vacuum drying promptly gets the ethanol extract of Olibanum supercritical extraction medicinal residues.
C, mixing: the gained clathrate is mixed with ethanol extract, promptly get Chinese medicine composition.
D, get the gained Chinese medicine composition, make pill according to a conventional method.
Embodiment 5
A. Olibanum supercritical extraction, enclose: Olibanum (system) crushed after being dried is become 50 purpose fine powders, CO
2Supercritical extraction, extracting pressure are 28Mpa, and extraction temperature is 40 ℃, CO
2Flow velocity be 15L/h, extracted the separated and collected extract 1 hour.Extract adds the anhydrous alcohol solution of extract weight 15%, gets the water dissolution of the beta-schardinger dextrin-of 7 times of amounts of extract weight with 3 times of amounts of beta-schardinger dextrin-weight, adopts polishing to carry out enclose, and the enclose time is 40 minutes, and vacuum drying promptly gets Olibanum CO
2The clathrate of supercritical extract.
B. alcohol extraction: the medicinal residues of Olibanum (system) supercritical extraction are that 65% ethanol stirring and refluxing is extracted 2 times with the concentration of 7 times of amounts of medicinal residues weight, each 1.5 hours, left standstill 48 hours, draw supernatant, filter, filtrate decompression is concentrated into the clear paste of relative density 1.20 (60 ℃), and vacuum drying promptly gets the ethanol extract of Olibanum supercritical extraction medicinal residues.
C, mixing: the gained clathrate is mixed with ethanol extract, promptly get Chinese medicine composition.
D, get the gained Chinese medicine composition, make drop pill according to a conventional method.
Claims (4)
1, a kind of Chinese medicine composition is characterized in that said composition obtains through the following steps:
A. Olibanum supercritical extraction, enclose: the Olibanum (processed) crushed after being dried is become 40～80 purpose granules, CO
2Supercritical extraction, extracting pressure are 18～34Mpa, and extraction temperature is 35～60 ℃, CO
2Flow velocity be 10～30L/h, extracted the separated and collected extract 0.5～2.5 hour; Extract adds the anhydrous alcohol solution of extract weight 10～30%, get the water dissolution of the beta-schardinger dextrin-of 6～10 times of amounts of extract weight with 1～3 times of beta-schardinger dextrin-weight, adopt polishing to carry out enclose, the enclose time is 20～40 minutes, and vacuum drying promptly gets Olibanum CO
2The clathrate of supercritical extract;
B. alcohol extraction: the medicinal residues of Olibanum (processed) supercritical extraction are that the ethanol stirring and refluxing of 50-70% is extracted 1～3 time with the concentration of 6～10 times of amounts of medicinal residues weight, each 1～2 hour, left standstill 12～48 hours, draw supernatant, filter, it is 1.15～1.25 clear paste that filtrate decompression is concentrated into 60 ℃ of relative densities, and vacuum drying promptly gets the ethanol extract of Olibanum supercritical extraction medicinal residues;
C. mix: clathrate and ethanol extract are mixed, promptly get described Chinese medicine composition.
2,, it is characterized in that said composition has following dosage form: pill, tablet, powder, granule, capsule according to the described Chinese medicine composition of claim 1.
3, the preparation technology of the described Chinese medicine composition of claim 1 is characterized in that this preparation technology comprises the following steps:
A. Olibanum supercritical extraction, enclose: the Olibanum (processed) crushed after being dried is become 40～80 purpose granules, CO
2Supercritical extraction, extracting pressure are 18～34Mpa, and extraction temperature is 35～60 ℃, CO
2Flow velocity be 10～30L/h, extracted the separated and collected extract 0.5～2.5 hour; Extract adds the anhydrous alcohol solution of extract weight 10～30%, get the water dissolution of the beta-schardinger dextrin-of 6～10 times of amounts of extract weight with 1～3 times of beta-schardinger dextrin-weight, adopt polishing to carry out enclose, the enclose time is 20～40 minutes, and vacuum drying promptly gets Olibanum CO
2The clathrate of supercritical extract;
B. alcohol extraction: the medicinal residues of Olibanum (processed) supercritical extraction are that the ethanol stirring and refluxing of 50-70% is extracted 1～3 time with the concentration of 6～10 times of amounts of medicinal residues weight, each 1～2 hour, left standstill 12～48 hours, draw supernatant, filter, it is 1.15～1.25 clear paste that filtrate decompression is concentrated into 60 ℃ of relative densities, and vacuum drying promptly gets the ethanol extract of Olibanum supercritical extraction medicinal residues;
C. mix: clathrate and ethanol extract are mixed, get final product.
4, the application of the described Chinese medicine composition of claim 1 in preparation analgesia, anti-inflammatory drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610096418A CN100581557C (en) | 2006-09-25 | 2006-09-25 | Chinese medicinal composition, its preparation and use in pharmacy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610096418A CN100581557C (en) | 2006-09-25 | 2006-09-25 | Chinese medicinal composition, its preparation and use in pharmacy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1939377A CN1939377A (en) | 2007-04-04 |
| CN100581557C true CN100581557C (en) | 2010-01-20 |
Family
ID=37958079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200610096418A Active CN100581557C (en) | 2006-09-25 | 2006-09-25 | Chinese medicinal composition, its preparation and use in pharmacy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100581557C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104324066B (en) * | 2014-02-17 | 2017-04-19 | 广东省中医药工程技术研究院 | Preparation method of frankincense particles |
| CN105586151B (en) * | 2015-12-30 | 2020-07-14 | 南京中山制药有限公司 | Extraction method of frankincense volatile oil |
| CN117338829B (en) * | 2023-09-21 | 2024-05-14 | 珠海安生凤凰制药有限公司 | Traditional Chinese medicine inclusion compound and preparation method and application thereof |
-
2006
- 2006-09-25 CN CN200610096418A patent/CN100581557C/en active Active
Non-Patent Citations (4)
| Title |
|---|
| SFE等4种制备工艺对乳香镇痛、抗炎作用的影响. 郑方明等.南京中医药大学学报，第19卷第4期. 2003 |
| SFE等4种制备工艺对乳香镇痛、抗炎作用的影响. 郑方明等.南京中医药大学学报，第19卷第4期. 2003 * |
| 中药药剂学. 张兆旺等，364，中国中医药出版社. 2003 |
| 中药药剂学. 张兆旺等，364，中国中医药出版社. 2003 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1939377A (en) | 2007-04-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102430090B (en) | Traditional Tibetan medicine Ruyizhenbao composite preparation and preparation method thereof | |
| CN101274012B (en) | Composition of Prunella plant extracts, preparation and pharmaceutical use thereof | |
| CN104042577A (en) | Stable topiroxostat tablet and preparation method thereof | |
| JP2016539173A (en) | Oral solid preparations containing broad-kind grasses and total flavonoids, and uses thereof | |
| CN100581557C (en) | Chinese medicinal composition, its preparation and use in pharmacy | |
| CN102342945B (en) | Application of Cortex Ilicis Rotundae saponin compound in preparing anti-inflammatory and analgetic medicament | |
| CN102370635A (en) | Application and preparation method of novel nardosinone | |
| CN104000797A (en) | Pharmaceutic preparation including mangiferin glycoside and preparation method thereof | |
| CN104666585A (en) | Application of radix rehmanniae leaf extract in preparation of blood glucose-reducing medicines | |
| CN102772497B (en) | Alcohol extraction effective part of medicinal material of Relinqing granules and preparation method thereof | |
| CN103549434A (en) | Making method of corn stigma effervescent tablets | |
| CN102283874B (en) | Method for preparing taxillus chinensis danser extract and application | |
| CN102188483B (en) | Extract for treating pharyngolaryngitis and preparation method thereof | |
| CN100553648C (en) | A kind of Chinese medicine composition and preparation technology thereof and the application in pharmacy | |
| CN102552491B (en) | Dai-dai fruit total flavonoid self-microemulsion micro pill and preparation method thereof | |
| CN105687274A (en) | Novel application of duramen of Acanthopanax giraldii Harms or extract of duramen of Acanthopanax giraldii Harms | |
| Li et al. | Research of Morinda officinalis how’s oligosaccharide extraction and antidepressant effects | |
| CN100435809C (en) | Ginseng bupleurum drop pill for treating liver disease and its preparation method | |
| CN104116753A (en) | Application of aucubin to preparation of medicines for treating idiopathic pulmonary fibrosis | |
| CN104288142B (en) | The xylocarpus granatum element H and the like purposes in preparing anti-depression drug or food | |
| CN104784257A (en) | Preparation method of traditional Chinese medicine cortex magnoliae officinalis ultrafine powder and dissolution rate determination method | |
| CN101670020A (en) | Composition for treating premenstrual syndrome | |
| CN101554397B (en) | Applications of eucommia ulmoide total alkaloid | |
| CN101199540B (en) | Polygonin sheet and preparing method thereof | |
| CN101531616B (en) | Method for preparing effective ingredient with antitussive effect, namely 4-Hydroxybenzyl cyanide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |