CN100578223C - Agarose gel film substrate, its production and use - Google Patents
Agarose gel film substrate, its production and use Download PDFInfo
- Publication number
- CN100578223C CN100578223C CN200610065057A CN200610065057A CN100578223C CN 100578223 C CN100578223 C CN 100578223C CN 200610065057 A CN200610065057 A CN 200610065057A CN 200610065057 A CN200610065057 A CN 200610065057A CN 100578223 C CN100578223 C CN 100578223C
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- Prior art keywords
- substrate
- agarose
- row
- gel
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000000034 method Methods 0.000 claims description 55
- 239000000499 gel Substances 0.000 claims description 50
- 238000002360 preparation method Methods 0.000 claims description 33
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- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 claims description 20
- 238000007254 oxidation reaction Methods 0.000 claims description 15
- 239000002002 slurry Substances 0.000 claims description 15
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- 229910052751 metal Inorganic materials 0.000 claims description 4
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- -1 polypropylene Polymers 0.000 claims description 4
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- 239000000523 sample Substances 0.000 abstract description 23
- 239000012472 biological sample Substances 0.000 abstract description 7
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 8
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- 241000872931 Myoporum sandwicense Species 0.000 description 3
- 239000002033 PVDF binder Substances 0.000 description 3
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- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 2
- 125000004414 alkyl thio group Chemical group 0.000 description 2
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- 239000007788 liquid Substances 0.000 description 2
- 229920003052 natural elastomer Polymers 0.000 description 2
- 229920001194 natural rubber Polymers 0.000 description 2
- 229920002120 photoresistant polymer Polymers 0.000 description 2
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
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- 238000007790 scraping Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000001338 self-assembly Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000004593 Epoxy Substances 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
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- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000001259 photo etching Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920002627 poly(phosphazenes) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 238000006884 silylation reaction Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
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Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200610065057A CN100578223C (en) | 2006-03-17 | 2006-03-17 | Agarose gel film substrate, its production and use |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200610065057A CN100578223C (en) | 2006-03-17 | 2006-03-17 | Agarose gel film substrate, its production and use |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1818649A CN1818649A (en) | 2006-08-16 |
CN100578223C true CN100578223C (en) | 2010-01-06 |
Family
ID=36918748
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200610065057A Expired - Fee Related CN100578223C (en) | 2006-03-17 | 2006-03-17 | Agarose gel film substrate, its production and use |
Country Status (1)
Country | Link |
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CN (1) | CN100578223C (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101629955B (en) * | 2009-08-07 | 2012-09-26 | 中国海洋大学 | Fish lymphocystis disease virus (LCDV) immunity detection chip and preparation method and application thereof |
CN101629954B (en) * | 2009-08-07 | 2012-09-26 | 中国海洋大学 | Immunity detection chip of prawn white spot syndrome virus (WSSV) and preparation method thereof and application |
CN109827814A (en) * | 2019-03-11 | 2019-05-31 | 南京大学 | A kind of sampling film preparation of novel air particulate matter agar and exempt from solvent extraction cell process for exposing |
CN112630420B (en) * | 2020-12-07 | 2022-10-14 | 北京科技大学 | Method for realizing directional coupling by using glycosyl of antibody and solid-phase carrier material |
-
2006
- 2006-03-17 CN CN200610065057A patent/CN100578223C/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
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CN1818649A (en) | 2006-08-16 |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee |
Owner name: CAPITALBIO CORPORATION CO., LTD. Free format text: FORMER NAME: CAPITALBIO CORPORATION |
|
CP01 | Change in the name or title of a patent holder |
Address after: 102206 Beijing City, Changping District Life Science Park Road No. 18 Patentee after: CAPITALBIO Corp. Patentee after: TSINGHUA University Address before: 102206 Beijing City, Changping District Life Science Park Road No. 18 Patentee before: CAPITALBIO Corp. Patentee before: Tsinghua University |
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100106 |
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CF01 | Termination of patent right due to non-payment of annual fee |