CN100577789C - The method of Artifically-cultured Chinese caterpillar fungus - Google Patents
The method of Artifically-cultured Chinese caterpillar fungus Download PDFInfo
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- CN100577789C CN100577789C CN200510034483A CN200510034483A CN100577789C CN 100577789 C CN100577789 C CN 100577789C CN 200510034483 A CN200510034483 A CN 200510034483A CN 200510034483 A CN200510034483 A CN 200510034483A CN 100577789 C CN100577789 C CN 100577789C
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Abstract
A kind of method of Artifically-cultured Chinese caterpillar fungus, it is that bacterial classification inoculation with Cordyceps militaris (L.) Link. is on rice medium, send out bacterium, illumination annesl through the darkroom, and when fruit body primordium forms, substratum is relayed on the rack from culturing bottle, make the end, face and the periphery of substratum all grow sporophore.After gathering for the first time, remove the old mycelia on the substratum, spray nutritive water, can grow sporophore for the second time after 20 days again.The present invention is than adopting glass preserving jar production less investment, and cost is low, and technology is simple, amounts to biological transformation ratio more than 150%, and the many 1-2 of rate ratio prior art doubly.
Description
Technical field
The present invention relates to the method for a kind of method of plant growing, particularly a kind of Artifically-cultured Chinese caterpillar fungus.
Background technology
Cordyceps is the distinctive Chinese medicine rarity of China, is Clavicipitaceae, Cordyceps, and more famous has two kinds, and a kind of is the Cordyceps sinensis that is grown on the Qinghai-Tibet mountain region; Another kind is the Cordyceps militaris (L.) Link. that is grown in the Northeast, two kinds chemical ingredients and amino acid, micro-basic identical, the actives that all contains number of different types such as the distinctive cordycepic acid of Chinese caterpillar fungus, cordycepin, Cordyceps polysaccharide, adenosine and superoxide-dismutase makes Cordyceps have extensively and significantly dietotherapy effect.Modern study proves that Cordyceps also has multiple pharmacological effect except that benefiting action; Bronchial smooth muscle there is tangible dilating effect, can strengthens adrenergic effect, be used for the treatment of chronic bronchitis, obstructive emphysema, pulmonary tuberculosis etc., Cordyceps can suppress heart, makes decreased heart rate, is used for the treatment of irregular pulse, essential hypotension etc.; Energy enhance immunity function delays senility, and suppresses tumour cell, and can improve the untoward reaction that chemotherapy causes, can be used for treating the complex therapy of multiple difficult and complicated illness such as neurasthenia.Be used for the postoperative recovery physical efficiency, more can show remarkable effect and since Cordyceps improve the health, disease preventing and treating, antifatigue, aspect effect brilliance such as promote longevity, by ancient Chinese medicine doctor is praised highly.It is special that Cordyceps sinensis requires ecotope, and strict parasitics is arranged again, and it is difficult to gather, resource scarcity, and prices are rather stiff.In recent years, owing to illegally excavate, make China's wild cordyceps resource be close to exhaustion.Country classifies it as Chinese Second Class Key Protected Plant, and the order limit is adopted.The cultivation Cordyceps both can satisfy the growing needs of people, can protect the plateau vegetation again, and remarkable economic efficiency and social benefit are arranged.Prior art is technical ripe with the glass preserving jar Artifically-cultured Chinese caterpillar fungus, but is subjected at the bottom of the vial and the restriction of periphery, can only be at media surface eldest son entity, and biological transformation ratio the highlyest is no more than 80% generally about 50%.
Summary of the invention
The method that the purpose of this invention is to provide a kind of Artifically-cultured Chinese caterpillar fungus, it is that bacterial classification inoculation with Cordyceps militaris (L.) Link. is on rice medium, send out bacterium, illumination annesl through the darkroom, and when fruit body primordium forms, substratum is relayed on the rack from culturing bottle, make the end, face and the periphery of substratum all grow sporophore.After gathering for the first time, remove the old mycelia on the substratum, spray nutritive water, can grow sporophore for the second time after 20 days again.
The present invention includes following steps:
(1) preparation of substratum
Get peptone 10-30 gram, glucose 10-30 gram, dipotassium hydrogen phosphate 0.1-0.5 gram, sal epsom 0.1-1 gram, VITMAIN B1 0.1 gram earlier, be dissolved in 1000 ml tap waters and make nutrient solution, standby; Select for use the glass preserving jar of 500 milliliters of capacity to make culturing bottle again, every bottle is amplified rice 20-40 gram, nutrient solution 30-50 milliliter, and bottleneck polypropylene foil film envelope is tight;
(2) sterilization
Charged culturing bottle is placed in the steam pressure cooker, at 1.5kg/cm
2Pressure sterilization 60 minutes, take the dish out of the pot relief its lower the temperature naturally, standby;
(3) inoculation
After culturing bottle drops to room temperature, adopt syringe to penetrate the foil seal film and inject liquid cordyceps species 2-10 milliliter, seal pin hole with adhesive tape;
(4) culturing bottle with inoculation moves in the dark place, sends out bacterium for controlled temperature 10-25 ℃, and mycelia was covered with whole substratum in 12-15 days;
(5) after mycelia is covered with substratum, in the daytime adopt natural scattering illumination, the lighting in evening, maintenance light application time every day is no less than 14 hours, mycelia transfers orange to by white after 3-5 days, behind the annesl evening light requirement photograph not, keeping in the daytime, scattered-light intensity is the 100-500 lux, controlled temperature 10-25 ℃, the surface of 10-15 days substratum can form a meter granulous fruit body primordium;
(6) promptly be not bacterial contamination after the Cordyceps fruit body primordium forms, open the foil seal film, substratum pressed from both sides out be placed on the rack, controlled temperature 15-25 ℃, humidity 90-95%, 15-20 days, whole media surface all grow the sporophore of 6-8 cm long, and biological transformation ratio can reach more than 100%;
(7) the old mycelia of media surface is removed after gathering, sprayed 1-10% sucrose nutritive water, keep 20-25 ℃ of temperature, humidity grows second batch of sporophore after 90%, 20 day, and biological transformation ratio can reach more than 150%
The present invention is than adopting glass preserving jar production less investment, and cost is low, and technology is simple, amounts to biological transformation ratio more than 150%, and the many 1-2 of rate ratio prior art doubly.
Embodiment
Take by weighing peptone 20 grams, glucose 20 grams, dipotassium hydrogen phosphate 0.2 gram, sal epsom 0.5 gram, VITMAIN B1 0.1 gram earlier, be dissolved in 1000 ml tap waters and make nutrient solution, standby; Select for use the glass preserving jar of 500 milliliters of capacity to make culturing bottle again, every bottle is amplified rice 30 grams, 48 milliliters of nutrient solutions, and bottleneck polypropylene foil film envelope is tight, makes substratum;
Charged culturing bottle is placed in the steam pressure cooker, at 1.5kg/cm
2Pressure sterilization 60 minutes, take the dish out of the pot relief its lower the temperature naturally, standby;
After culturing bottle drops to room temperature, adopt syringe to penetrate the foil seal film and inject 5 milliliters of liquid cordyceps species, after the inoculation, seal pin hole with adhesive tape;
The culturing bottle of inoculation is moved in the dark place, and controlled temperature is sent out bacterium for 15 ℃, and mycelia was covered with whole substratum in 12-15 days;
Mycelia promptly enters the annesl stage after covering with substratum, in the daytime adopt natural scattering illumination, evening, light prolonged illumination, keep the every day light application time to be no less than 14 hours, mycelia transfers orange to by white after 3-5 days, and need not illumination evening behind the annesl, keeping in the daytime, scattered-light intensity is 200 luxs, controlled temperature is 22 ℃ in the daytime, reduces to 15 ℃ evening, and the surface of 10-15 days substratum can form a meter granulous fruit body primordium;
After forming, the Cordyceps fruit body primordium promptly is not bacterial contamination, rack on the bottle outlet, open the foil seal desert, the substratum that forms fruit body primordium pressed from both sides out be placed on the rack, the end, face and the periphery of controlled temperature 22-25 ℃, humidity 90%, 15-20 days, whole substratum all grow the Cordyceps sporophore of 6-8 cm long, and biological transformation ratio can reach more than 100%;
After gathering the old mycelia of media surface is removed, sprayed 5% sucrose nutritive water, keep 22-25 ℃ of temperature, humidity can grow second batch of Cordyceps sporophore after 90%, 20 day, amount to biological transformation ratio more than 150%, and the many 1-2 of rate ratio prior art doubly.
Claims (1)
1, a kind of method of Artifically-cultured Chinese caterpillar fungus is characterized in that comprising the steps:
(1) preparation of substratum
Get peptone 10-30 gram, glucose 10-30 gram, dipotassium hydrogen phosphate 0.1-0.5 gram, sal epsom 0.1-1 gram, VITMAIN B1 0.1 gram earlier and be dissolved in 1000 ml tap waters and make nutrient solution, standby; Select for use the glass preserving jar of 500 milliliters of capacity to make culturing bottle again, every bottle is amplified rice 20-40 gram, nutrient solution 30-50 milliliter, and bottleneck polypropylene foil film envelope is tight;
(2) sterilization
Charged culturing bottle is placed in the steam pressure cooker, at 1.5k g/cm
2Pressure sterilization 60 minutes, take the dish out of the pot relief its lower the temperature standby naturally;
(3) inoculation
After culturing bottle drops to room temperature, adopt syringe to penetrate the foil seal film and inject liquid cordyceps species 2-10 milliliter, seal pin hole with adhesive tape;
(4) culturing bottle with inoculation moves in the dark place, sends out bacterium for controlled temperature 10-25 ℃, and mycelia was covered with whole substratum in 12-15 days;
(5) after mycelia is covered with substratum, in the daytime adopt natural scattering illumination, the lighting in evening, maintenance light application time every day is no less than 14 hours, mycelia transfers orange to by white after 3-5 days, behind the annesl evening light requirement photograph not, keeping in the daytime, scattered-light intensity is the 100-500 lux, controlled temperature 10-25 ℃, the surface of 10-15 days substratum can form a meter granulous fruit body primordium;
(6) promptly be not bacterial contamination after the Cordyceps fruit body primordium forms, open the foil seal film, substratum pressed from both sides out be placed on the rack, controlled temperature 15-25 ℃, humidity 90-95%, 15-20 days, whole media surface all grow the sporophore of 6-8 cm long, and biological transformation ratio can reach more than 100%;
(7) the old mycelia of media surface is removed after gathering, sprayed 1-10% sucrose nutritive water, keep 20-25 ℃ of temperature, humidity grows second batch of sporophore after 90%, 20 day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200510034483A CN100577789C (en) | 2005-04-28 | 2005-04-28 | The method of Artifically-cultured Chinese caterpillar fungus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200510034483A CN100577789C (en) | 2005-04-28 | 2005-04-28 | The method of Artifically-cultured Chinese caterpillar fungus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1853458A CN1853458A (en) | 2006-11-01 |
| CN100577789C true CN100577789C (en) | 2010-01-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN200510034483A Expired - Fee Related CN100577789C (en) | 2005-04-28 | 2005-04-28 | The method of Artifically-cultured Chinese caterpillar fungus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102177817A (en) * | 2011-02-24 | 2011-09-14 | 湖北圣峰药业有限公司 | Method for cultivating cordyceps militaris rich in SOD (superoxide dismutase) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101066090B (en) * | 2007-05-28 | 2012-05-23 | 李治德 | Aweto activated reinforced immunological feed additive and its production process |
| CN103130550B (en) * | 2013-03-20 | 2015-04-01 | 上海市农业科学院 | Culture medium and culture method of male agaric mycelium |
| CN104304016B (en) * | 2014-09-30 | 2017-03-08 | 中山大学 | A kind of method that utilization cultivating chamber prepares aweto strain material |
-
2005
- 2005-04-28 CN CN200510034483A patent/CN100577789C/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 灵芝 虫草 天麻与蜜环菌. 严清波等，88-92，科学技术文献出版社. 2002 * |
| 虫草人工栽培技术. 李昊，58-63，金盾出版社. 2001 灵芝 虫草 天麻与蜜环菌. 严清波等，88-92，科学技术文献出版社. 2002 |
| 虫草人工栽培技术. 李昊，58-63，金盾出版社. 2001 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102177817A (en) * | 2011-02-24 | 2011-09-14 | 湖北圣峰药业有限公司 | Method for cultivating cordyceps militaris rich in SOD (superoxide dismutase) |
| CN102177817B (en) * | 2011-02-24 | 2012-08-22 | 湖北圣峰药业有限公司 | Method for cultivating cordyceps militaris rich in SOD (superoxide dismutase) |
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| Publication number | Publication date |
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| CN1853458A (en) | 2006-11-01 |
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