CN100564292C - A kind of animal dung urine deodorizing method and application thereof - Google Patents
A kind of animal dung urine deodorizing method and application thereof Download PDFInfo
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- CN100564292C CN100564292C CN 200710071380 CN200710071380A CN100564292C CN 100564292 C CN100564292 C CN 100564292C CN 200710071380 CN200710071380 CN 200710071380 CN 200710071380 A CN200710071380 A CN 200710071380A CN 100564292 C CN100564292 C CN 100564292C
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Abstract
A kind of animal dung urine deodorizing method and application thereof.It is characterized in that superoxide enzyme solution and 1%～4% superoxol of preparation 0.5U/mL～1.5U/mL earlier, on the livestock and poultry fecaluria, spray superoxide enzyme solution (every liter of fecaluria sprays 3mL) earlier, spray isopyknic superoxol again, or (every liter of fecaluria sprays 0.3g～0.5g) get final product to spray 75% calcium peroxide powder.Utilize the peroxidase can be in temperature, pH value, Pollutant levels and the salinity range of broad, with hydrogen peroxide or calcium peroxide is electron acceptor(EA), with the phenolic compound catalyzed oxidation is the principle that free radical decomposes removal, is used to drive away the foul smell that the livestock and poultry farm fecaluria produces.Deodorizing method is simple, without any need for the facility of complexity, needn't increase employee and equipment, no investment cost, and running cost is lower, and deodorizing effect is remarkable and permanent, can not cause secondary pollution.Have broad application prospects in this small profit trade of aquaculture.
Description
Technical field
The present invention relates to a kind of animal dung urine deodorizing method and application thereof.
Background technology
The development of the fast development, particularly large-scale cultivation of producing along with livestock and poultry breeding industry, the generation of a large amount of animal wastes causes the poultry industry development and the contradiction of environmental pollution to show especially day by day.The topmost pollutent of livestock and poultry breeding industry is the foul smell that fecaluria and anaerobically fermenting thereof produce.The main component of foul smell is voltaile fatty acid, indoles, phenolic compound, ammonia, volatile amine, volatile sulfur compounds etc.
Large-scale odor removal investment and running cost are all higher, and for the higher point source of emission concentration, effect is obvious.But to the uncontrollable discharge source of livestock and poultry farm and so on, collection and confinement of gases pipeline complexity, cost of investment increases greatly, and treatment effect is also undesirable.Existing market also has the reodorant of employing de-odorised, mainly contains chemical deodorizing agent, plant deodorant, microbial deodorant.Chemical deodorizing agent and plant deodorant are except that cost is higher, and the chemical deodorizing agent may produce secondary pollution, and the microbial deodorant cost is low than the above two, mainly are that flora is less, the cause that fermentation costs reduces, but flora I haven't seen you for ages reduces deodorizing effect.In addition, microbial deodorant can not long storage time, is difficult to long-distance transportation, and application is restricted.
Peroxidase can be an electron acceptor(EA) with hydrogen peroxide or calcium peroxide in temperature, pH value, Pollutant levels and the salinity range of broad, is that the phenol oxyradical decomposes removal with the phenolic compound catalyzed oxidation.Handle in the research and show containing phenol, amino benzene analog waste water, can remove phenol and aniline more than 30 kinds, the clearance of some phenol is reached 99%.
Summary of the invention
The purpose of this invention is to provide a kind of deodorizing method and application thereof of livestock and poultry fecaluria, it can overcome the above-mentioned shortcoming of prior art, and this deodorizing method is simple, and cost is lower, and deodorizing effect is remarkable, can not cause secondary pollution.Plant can needn't increase employee and equipment by oneself.
Animal dung urine deodorizing method of the present invention, it is characterized in that superoxide enzyme solution and 1%～4% superoxol of preparation 0.5U/mL～1.5U/mL earlier, on the livestock and poultry fecaluria, spray superoxide enzyme solution (every liter of fecaluria sprays 3mL) earlier, spray isopyknic superoxol again, or 75% calcium peroxide powder (every liter of fecaluria sprays 0.3g～0.5g) get final product.
The advantage of animal dung urine deodorizing method provided by the invention is:
1) without any need for the deodorizing facility, no cost of investment.Though the price of horseradish peroxidase is higher, consumption is few, also can select the lower soybean peel peroxidase of price or the isozyme of other peroxidase, so total cost is low.
2) do not produce secondary pollution, but also help the subsequent disposal utilization of fecaluria.The oxygen that peroxide breakdown produces can make oxygen content increase in the fecaluria, produces foul smell thereby reduce by the fecaluria anaerobically fermenting.The water that hydrogen peroxide decomposition produces does not have influence to environment.Calcium peroxide decomposes the physico-chemical property that the calcium ion that produces can improve soil, makes fecaluria land use preferably.
3) deodorizing effect is permanent, after the sprinkling, can keep not having stink 3 days.
4) the enzyme source of peroxidase is abundant, and storing is convenient.Livestock and poultry farm can spray and use voluntarily with using with joining, and is simple and convenient.
Embodiment
Following examples adopt the various foul smell compositions of gas-chromatography (flame photometric detector) assay determination, and the gasometry condition is as follows: vaporizer temperature and detector temperature are 220 ℃, and column temperature adopts temperature programming, and at first 35 ℃ kept 1 minute down; Heated up 7.67 minutes with 15 ℃/minute speed again, kept 2 minutes down at 150 ℃; Heated up 2.4 minutes with 25 ℃/minute speed again, until 210 ℃; Kept 5 minutes down at 210 ℃ at last.Sampling volume is 1 μ L, is carrier gas (the ND indicated concentration is lower than the detectability of instrument) with the helium.Odor concentration adopts sensory test method.
Embodiment 1: peroxidase concn is to the influence research of deodorizing effect
1) take by weighing different mass horseradish peroxidase powder preparation (250U/mg), water be mixed with different concns (each 50mL of superoxide enzyme solution of 0.1～3.0U/mL), standby.
2) get hydrogen peroxide strong solution (30%) 367mL, it is 10% dilute solution 1100mL that water is mixed with concentration, standby.
3) prepare 24 plastics bucket with covers (high 22cm, bottom internal diameter 18cm, top internal diameter 30cm), swine excrement and urine mixing and stirring are starchiness, in every bucket, put 3L.
4) the superoxide enzyme solution 15mL that above-mentioned different concns sprays in elder generation on the fecaluria of 21 buckets sprays the 15mL Hydrogen Peroxide Dilute Solution again, does replicate(determination) for 3 one group.Only spray distilled water 30mL on the fecaluria of 3 buckets in addition, in contrast.To cover immediately after spraying deodorizing solution, build.Extract 10mL fecaluria mixed solution after 2 hours, analyze the concentration of wherein several representative foul smell compositions.And differentiate the stench degree with sense organ.Treatment effect is shown in Table 1.
The deodorizing effect of the peroxidase of table 1 different concns on pig manure urine
As seen from Table 1, along with the raising of peroxidase concn, the concentration of various foul smell compositions all descends thereupon.Peroxidase concn surpasses after the 2.0U/mL, the fall of the concentration of various foul smell compositions is little, consider deodorizing effect and cost two aspects, therefore the superoxide enzyme solution is comparatively suitable with 0.5U/mL～1.5U/mL concentration, and the dosage of peroxidase is with every liter of fecaluria 3mL (about 2.5U～7.5U) be advisable.
Embodiment 2: superoxol concentration is to the influence research of deodorizing effect
1) take by weighing horseradish peroxidase powder preparation (250U/mg), water is mixed with the superoxide enzyme solution 350mL of 1.0U/mL concentration, and is standby.
2) get different volumes hydrogen peroxide strong solution (30%) 1～10mL, water is mixed with the dilute solution 50mL of 0.5%～6% concentration respectively, and is standby.
3) prepare 24 plastics bucket with covers (high 22cm, bottom internal diameter 18cm, top internal diameter 30cm).Swine excrement and urine mixing and stirring are starchiness, in every bucket, put 3L.
4) spray superoxide enzyme solution 15mL earlier on the fecaluria of 21 buckets, the Hydrogen Peroxide Dilute Solution of spraying the 15mL different concns is again done replicate(determination) for 3 one group.Only spray distilled water 30mL on the fecaluria of 3 buckets in addition, in contrast.Immediately lid is built after spraying deodorizing solution.Extract 10mL fecaluria mixed solution after 2 hours, analyze the concentration of wherein several foul smell compositions, and differentiate the stench degree with sense organ.Treatment effect is shown in Table 2.
The deodorizing effect of the hydrogen peroxide of table 2 different concns on pig manure urine
As seen from Table 2, along with the raising of superoxol concentration, the concentration of various foul smell compositions all descends thereupon.Superoxide hydrogen solution concentration surpasses after 4%, the fall of the concentration of various foul smell compositions is little, consider deodorizing effect and cost two aspects, so the superoxide hydrogen solution is comparatively suitable with 1%～4% concentration, the dosage of hydrogen peroxide is advisable with every liter of fecaluria 0.05～0.2g.
Embodiment 3: the calcium peroxide powder of different mass is to the influence research of deodorizing effect
1) take by weighing horseradish peroxidase powder preparation (250U/mg), water is mixed with the superoxide enzyme solution 350mL of 1.0U/mL concentration, and is standby.
2) prepare 24 plastics bucket with covers (high 22cm, bottom internal diameter 18cm, top internal diameter 30cm), swine excrement and urine mixing and stirring are starchiness, in every bucket, put 3L.
3) on the fecaluria of 21 buckets, spray superoxide enzyme solution 15mL earlier, spray 75% calcium peroxide powder of different mass again, do replicate(determination) for 3 one group.Only spray distilled water 15mL on the fecaluria of 3 buckets in addition, in contrast.Immediately lid is built after spraying deodorizing solution.10mL fecaluria mixed solution after 2 hours is analyzed the concentration of wherein several foul smell compositions, and differentiates the stench degree with sense organ.Treatment effect is shown in Table 3.
The deodorizing effect of the calcium peroxide of table 3 different mass on pig manure urine
As seen from Table 3, along with the raising of calcium peroxide sprinkling quality, the concentration of various foul smell compositions all descends thereupon.The superoxide calcium peroxide sprays quality and surpasses after the 1.5g, and the fall of the concentration of various foul smell compositions is little, considers deodorizing effect and cost two aspects, and therefore when spraying calcium peroxide, it is comparatively suitable to spray 0.3～0.5g with every liter of fecaluria.
Embodiment 4: the deodorizing effect comparative studies of calcium peroxide and hydrogen peroxide
1) take by weighing horseradish peroxidase powder preparation (250U/mg), water is mixed with the superoxide enzyme solution 100mL of 1.0U/mL concentration, and is standby.
2) get certain volume hydrogen peroxide strong solution (30%), water is mixed with the dilute solution 50mL of 4% concentration respectively, and is standby.
3) prepare 9 plastics bucket with covers (high 22cm, bottom internal diameter 18cm, top internal diameter 30cm), swine excrement and urine mixing and stirring are starchiness, in every bucket, put 3L.
4) on the fecaluria of 6 buckets, spray earlier superoxide enzyme solution 15mL, spray the 15mL Hydrogen Peroxide Dilute Solution on 3 buckets therein again, spray 1.5g 75% calcium peroxide powder on 3, do replicate(determination) for 3 one group at other.Only spray distilled water 30mL on the fecaluria of 3 buckets in addition, in contrast.Immediately lid is built after spraying deodorizing solution.Extract 10mL fecaluria mixed solution after 2 hours, analyze the concentration of wherein several foul smell compositions, and differentiate the stench degree with sense organ.Treatment effect is shown in Table 4.
The different electron acceptor(EA)s of table 4 compare the deodorizing effect of pig manure urine
As seen from Table 4, hydrogen peroxide and calcium peroxide can be as the electron acceptor(EA)s of peroxidase, and under the situation of identical peroxide radical ion content, the deodorizing effect of calcium peroxide is better than hydrogen peroxide.Plant can select a kind of according to practical situation and the running cost of self voluntarily.
Embodiment 5: peroxidase long-acting deodorization Research on effect
1) take by weighing horseradish peroxidase powder preparation (250U/mg), water is mixed with the superoxide enzyme solution 250mL of 1.0U/mL concentration, and is standby.
2) get certain volume hydrogen peroxide strong solution (30%), water is mixed with the dilute solution 250mL of 4% concentration respectively, and is standby.
3) prepare 3 plastics bucket with covers (high 22cm, bottom internal diameter 18cm, top internal diameter 30cm), swine excrement and urine mixing and stirring are starchiness, in every bucket, put 3L.
4) on fecaluria, spray superoxide enzyme solution 15mL earlier, go up again and spray the 15mL Hydrogen Peroxide Dilute Solution, immediately lid is built after the spray solution.Before spraying, spray after 2 hours, 24 hours, 48 hours, 72 hours, 96 hours and extract 10mL fecaluria mixed solution respectively, analyze the concentration of wherein several foul smell compositions, and differentiate the stench degree, and differentiate the stench degree with sense organ with sense organ.Treatment effect is shown in Table 5.
The deodorizing effect of different test durations of table 5 to pig manure urine compares
As seen from Table 5, behind sprinkling peroxidase and the hydrogen peroxide, the foul smell composition descends significantly, and each composition just gos up to some extent after 72 hours, and this shows that the deodorizing time can reach 72 hours (about 3 days).
Claims (4)
1, a kind of animal dung urine deodorizing method, it is characterized in that on the livestock and poultry fecaluria, spraying earlier the superoxide enzyme solution of 0.5U/mL～1.5U/mL, every liter of fecaluria sprays 3mL, spraying isopyknic concentration again is 1%～4% superoxol, or 75% calcium peroxide powder, every liter of fecaluria sprays 0.3～0.5g and gets final product.
2, a kind of animal dung urine deodorizing method according to claim 1 is characterized in that peroxidase can adopt horseradish peroxidase, also can select the soybean peel peroxidase for use, or the isozyme of other peroxidase.
3, a kind of animal dung urine deodorizing method according to claim 1, the dosage that it is characterized in that hydrogen peroxide is every liter of fecaluria 0.05～0.2g.
4, a kind of livestock and poultry fecaluria deodorizing according to claim 1 is used, and it is characterized in that the application on feces of livestock and poultry, urine, slurry of feces and urine, compost deodorizing.
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| CN 200710071380 CN100564292C (en) | 2007-09-21 | 2007-09-21 | A kind of animal dung urine deodorizing method and application thereof |
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| CN101172745A CN101172745A (en) | 2008-05-07 |
| CN100564292C true CN100564292C (en) | 2009-12-02 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101954105A (en) * | 2010-09-16 | 2011-01-26 | 宁波工程学院 | Method for removing odor of feces and urine of animals and poultry through oxidation |
| CN103242070A (en) * | 2012-02-06 | 2013-08-14 | 高均 | Waste chemical reaction processor and waste processing method |
| CN105854059B (en) * | 2016-06-23 | 2019-04-23 | 南京首帆环保科技有限公司 | House refuse composite Nano deodorant and its preparation and application |
| CN106277353A (en) * | 2016-08-25 | 2017-01-04 | 南丹县环境卫生管理站 | A kind of deodorizing method of refuse depot |
| CN110143830B (en) * | 2019-06-28 | 2021-09-10 | 甘肃省农业科学院旱地农业研究所 | Application of peroxidase in compost, compost preparation method and application of compost |
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