CN100559157C - Utilize discarded ion exchange resin ball to measure the method for soil available phosphorus - Google Patents
Utilize discarded ion exchange resin ball to measure the method for soil available phosphorus Download PDFInfo
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- CN100559157C CN100559157C CNB2008100593425A CN200810059342A CN100559157C CN 100559157 C CN100559157 C CN 100559157C CN B2008100593425 A CNB2008100593425 A CN B2008100593425A CN 200810059342 A CN200810059342 A CN 200810059342A CN 100559157 C CN100559157 C CN 100559157C
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- ion exchange
- resin ball
- exchange resin
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Abstract
The invention discloses a kind of method of utilizing discarded ion exchange resin ball mensuration soil available phosphorus, may further comprise the steps: 1) discarded ion exchange resin ball is carried out Regeneration Treatment; 2) adopt experimental determination method or employing field in-site detecting method; 3) resin balls of taking out is cleaned with small amount of deionized water earlier; And then utilizing concentration to carry out wash-out for the hydrochloric acid solution of 0.5mol/L, co-elute 2 times is collected all eluents; 4) measure phosphorus concentration in the eluent with conventional molybdenum antimony resistance colorimetric method.The discarded ion exchange resin ball of utilization of the present invention is measured the method for soil available phosphorus, and is with low cost, easy and simple to handle, and test result is accurate.
Description
Technical field
The present invention relates to a kind of to regeneration of discarding ion exchange resin ball and the method for utilizing regenerating resin ball mensuration soil available phosphorus.
Background technology
Soil available phosphorus is also referred to as rapid available phosphorus, is can be comprised whole water-soluble phosphoruses, part ADSORPTION STATE phosphorus and easy mineralising attitude organophosphorus by the phosphorus component of plant absorbing in the soil.The content of soil available phosphorus has reflected the phosphorus supply ability of soil to this season crop to a certain extent, is an important indicator judging the soil fertility height, and general index according to soil available phosphorus instructs and uses phosphate fertilizer in agricultural production.
The assay method of soil available phosphorus is a lot, since the difference of the digestion agent of selecting, the difference of extracting method, and the application of measurement result is also inequality.The selection of digestion agent is decided according to soil property, and the method for the mensuration calcareous of laboratory routine and neutral soil rapid available phosphorus adopts the Olsen method at present, promptly utilizes the NaHCO of 0.5mol/L
3(pH8.5) make extraction agent and extract available phosphorus in the soil; For subacidity soil, 0.03NNH commonly used
4F-0.025NHCl lixiviate (Bray-I method) also has the Olsen of employing method.The distinct disadvantage of these conventional methods is influences that measurement result is subject to the factors such as extracting condition such as native liquor ratio, temperature, time, mode of oscillation.The application ion exchange resin ball that is grown up by the people such as professor Skogely of the upright university of Montana, United States is measured the method for available nutrients ion in recent years, more near the actual conditions of soil one plant system, therefore and be applicable to all soil, more and more accepted by agriculturist in the world.But resin balls costs an arm and a leg (4 dollars of every prices of " general good " resin balls), and after using once, promptly pass into disuse, cause analysis cost too high.
Summary of the invention
The technical problem to be solved in the present invention provides the method that the discarded ion exchange resin ball of a kind of with low cost, easy and simple to handle, accurate test result utilization is measured soil available phosphorus.
In order to solve the problems of the technologies described above, the invention provides a kind of method of utilizing discarded ion exchange resin ball mensuration soil available phosphorus, may further comprise the steps:
1), discarded ion exchange resin ball is carried out Regeneration Treatment:
Discarded ion exchange resin ball is soaked in the sodium bicarbonate (NaHCO that concentration is 1.0mol/L in the mode that stirs
3) 2h in the solution; Be soaked in the NaHCO that concentration is 0.1mol/L in the mode that stirs again after the taking-up
32h in the solution; Then use deionized water rinsing 1～2 time, with the unnecessary NaHCO of flush away
3Be soaked at last in the deionized water, get the regenerating resin ball, stand-by;
2), adopt the experimental determination method: claim 1.00g soil to place container, add deionized water 30ml, and then add 1 in regenerating resin ball; With the 2h that vibrates behind the container closure, take out resin balls;
Perhaps adopt field in-site detecting method: regenerating resin ball ball is placed soil table 7.5cm place down, on schedule after, the taking-up resin balls;
3), the resin balls of taking out is cleaned with small amount of deionized water earlier; And then utilizing concentration to carry out wash-out for the hydrochloric acid solution of 0.5mol/L, co-elute 2 times is collected all eluents;
4), measure phosphorus (P) concentration in the eluent with conventional molybdenum antimony resistance colorimetric method.
Measure the improvement of the method for soil available phosphorus as the discarded ion exchange resin ball of utilization of the present invention: discarded ion exchange resin ball is discarded " general good " resin balls.
Measure the further improvements in methods of soil available phosphorus as the discarded ion exchange resin ball of utilization of the present invention: in the step 3), each resin balls of cleaning is put into a 60ml plastic bottle respectively carry out wash-out, each wash-out uses the hydrochloric acid solution and the 0.5h that vibrates of the 0.5mol/L of 30ml, with the phosphorus of wash-out resin adsorption.
The characteristics of the inventive method are earlier discarded " general good " resin balls to be carried out Regeneration Treatment, utilize ion exchange principle then, and the simulating plant root system is put into soil to the absorption of phosphate anion with resin balls, and both are fully contacted; The phosphate anion that adsorbs on the wash-out resin balls again.The assay method of soil available phosphorus of the present invention by to discarded " general good " (UNIBEST) resin balls reach the purpose of using repeatedly more from birth, the resin balls of i.e. use regeneration is measured the active phosphorus in the soil, method is easy, quick, test result is accurate, with low cost, therefore have broad application prospects.The inventive method can not only be applicable to the mensuration of the available phosphorus of all kinds soil simultaneously, and can be used for the monitoring of field original position soil available phosphorus.
Embodiment
Embodiment 1, a kind of method of utilizing discarded ion exchange resin ball mensuration soil available phosphorus, carry out following steps successively:
1), discarded ion exchange resin ball is carried out Regeneration Treatment:
100 discarded " general good " ion exchange resin balls are soaked in the NaHCO that 1L concentration is 1.0mol/L in the mode that stirs
32h in the solution; Be soaked in the NaHCO that 2.5L concentration is 0.1mol/L in the mode that stirs again after the taking-up
32h in the solution; Then use deionized water rinsing 1～2 time, with the unnecessary NaHCO of flush away
3Be soaked at last in the deionized water, get the regenerating resin ball, stand-by;
2), adopt the experimental determination method:
Select representational rice terrace piece to arrange 18 at the Jinhua Wucheng District and execute the NK treatment region, sub-district area 100m
2Nitrogenous fertilizer urea, early rice are executed pure N 150kg/ha, and late rice is executed pure N 180kg/ha; Potash fertilizer is executed potassium chloride, and early late rice is executed pure K 60kg/ha respectively.Rice maturity sampling and measuring plant overground part absorbs the phosphorus amount.Before test, get table soil (0-20cm) soil sample, air-dry, ground the 2mm sieve.
Select the plastic bottle (each soil repeats once) of 36 60ml for use, in each plastic bottle, put into 1.00g soil, 30ml deionized water and 1 above-mentioned steps 1) the regenerating resin ball of gained.With plastic bottle sealing back vibration 2h, take out resin balls.
3), the resin balls of taking out is carefully cleaned with small amount of deionized water earlier, to remove the soil on surface.In each 60ml plastic bottle, put into an above-mentioned steps 2 then) resin balls of gained, utilize concentration to carry out wash-out again for the HCl solution of 0.5mol/L, co-elute 2 times desorbs the phosphate anion of resin balls absorption guaranteeing; Collect all eluents.Each wash-out adds 30ml 0.5mol/L HCl solution, and vibration 0.5h is in order to the P of wash-out resin adsorption.
4), measure phosphorus concentration in the eluent with conventional molybdenum antimony resistance colorimetric method.
The result shows that available phosphorus that resin balls is extracted and paddy rice inhale the phosphorus amount and be remarkable positive correlation, related coefficient=0.60.
Embodiment 2, a kind of method of utilizing discarded ion exchange resin ball mensuration soil available phosphorus, carry out following steps successively:
1), discarded ion exchange resin ball is carried out Regeneration Treatment:
With embodiment 1.
2), adopt the experimental determination method:
Select representational rice terrace piece to arrange 24 at the Jinhua Wucheng District and execute the NPK treatment region, sub-district area 45m
2Nitrogenous fertilizer urea, early rice are executed pure N 150kg/ha, and late rice is executed pure N 180kg/ha; Phosphate fertilizer calcium superphosphate, early late rice is executed K 25kg/ha respectively; Potash fertilizer is executed potassium chloride, and early late rice is executed pure K 60kg/ha respectively.In rice tillering mid-term, measure the plant overground part respectively at panicle primordium dif ferentiation stage and heading stage and absorb the phosphorus amount.
In rice tillering mid-term, respectively 4 regenerating resin balls are uniformly distributed in soil table 7.5cm place down at panicle primordium dif ferentiation stage and heading stage, after one day and seven days, respectively take out two of resin balls again;
3), with embodiment 1
4), measure phosphorus concentration in the eluent with conventional molybdenum antimony resistance colorimetric method.
The result shows that available phosphorus that resin balls was extracted in a day in the rice growing season and paddy rice inhale the phosphorus amount and be extremely significantly positive correlation, related coefficient=0.64; Available phosphorus that resin balls was extracted in seven days in the rice growing season and paddy rice inhale the phosphorus amount and are extremely significantly positive correlation, related coefficient=0.65;
The relation of embodiment 3, soil available phosphorus and soil sticking attitude phosphorus
1, measure the adsorption isothermal of 20 kinds of soil:
Every kind of soil is all done following processing:
Choose the 6g soil of every kind of soil, 6 control groups are set respectively; The 1g soil that is about to every kind of soil is placed in the 50ml centrifuge tube.Add 25ml respectively and contain the 0.02mol/L KCl solution of different phosphate concentration in these 6 centrifuge tubes, making the P concentration scale in these 6 centrifuge tubes is 0,5,10,15,20,30mg/L.
Do a repetition again according to above-mentioned processing then, to guarantee the positive parasexuality of result of calculation.
Then above-mentioned centrifuge tube is descended vibration 24h at 25 ℃ respectively, centrifugal, measure P concentration in the centrifugate, calculate the adsorbance of soil to phosphorus.
2, the soil of the residue in each centrifuge tube is washed 1 time with 10ml alcohol, discarded washing lotion; The regenerating resin ball that in each centrifuge tube, adds 25ml 0.02mol/L KCl solution and 1 step 1) gained of the present invention then; Then vibrate down and extract 16h at 25 ℃.
Take out resin balls, again by step 3) of the present invention and 4) measure the soil available phosphorus that resin balls is extracted.
Experimental result shows that all soil available phosphorus and soil sticking phosphorus that extract for examination soil resin balls all are remarkable positive correlation, and related coefficient is between 0.972-0.999.
The release rule of soil available phosphorus under embodiment 4, the different temperatures
Adopt discarded " general good " resin balls of regeneration to measure the rice soil dynamic law that soil available phosphorus discharges under different temperatures.
Select 3 diameter 20cm for use, the plastic containers of dark 28cm are as vessel.
In each vessel, add wind desiceted soil 2.5kg, add water and stir evenly, keep water layer 5cm.In each vessel, put into 6 in 6 in " general good " regenerating resin ball of step 1) gained of the present invention and " general good " commercial resin ball, make above-mentioned resin balls place 7.5cm soil layer depths.Again 3 vessel are placed 15 ℃ respectively, 25 ℃, in 35 ℃ of incubators.After waterflooding 1,7,14,21,28,35d respectively takes out 1 " general good " regenerating resin ball and 1 " general good " commercial resin ball respectively.
And then according to step 3) of the present invention and 4) handle.
The result shows that regeneration back resin balls is fit to power equation: RAQ equally to the extracted amount (RAQP) of soil available phosphorus and relation and " general good " commercial resin ball of incubation time under different temperatures
(i, t)=at
b
At last, it is also to be noted that what more than enumerate only is several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (2)
1, a kind of method of utilizing discarded ion exchange resin ball mensuration soil available phosphorus is characterized in that may further comprise the steps:
1), discarded ion exchange resin ball is carried out Regeneration Treatment:
Discarded ion exchange resin ball is soaked in 2h in the sodium bicarbonate solution that concentration is 1.0mol/L in the mode that stirs; Be soaked in 2h in the sodium bicarbonate solution that concentration is 0.1mol/L in the mode that stirs again after the taking-up; Then use deionized water rinsing 1～2 time, with the unnecessary sodium bicarbonate of flush away; Be soaked at last in the deionized water, get the regenerating resin ball, stand-by; Described discarded ion exchange resin ball is discarded " general good " resin balls;
2), adopt the experimental determination method: claim 1.00g soil to place container, add deionized water 30ml, and then add 1 in regenerating resin ball; With the 2h that vibrates behind the container closure, take out resin balls;
Perhaps adopt field in-site detecting method: the regenerating resin ball is placed soil table 7.5cm place down, on schedule after, the taking-up resin balls;
3), the resin balls of taking out is cleaned with small amount of deionized water earlier; And then utilizing concentration to carry out wash-out for the hydrochloric acid solution of 0.5mol/L, co-elute 2 times is collected all eluents;
4), measure phosphorus concentration in the eluent with conventional molybdenum antimony resistance colorimetric method.
2, the discarded ion exchange resin ball of utilization according to claim 1 is measured the method for soil available phosphorus, it is characterized in that: in the described step 3), each resin balls of cleaning is put into a 60ml plastic bottle respectively carry out wash-out, each wash-out uses the hydrochloric acid solution and the 0.5h that vibrates of the 0.5mol/L of 30ml, with the phosphorus of wash-out resin adsorption.
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| CNB2008100593425A CN100559157C (en) | 2008-01-25 | 2008-01-25 | Utilize discarded ion exchange resin ball to measure the method for soil available phosphorus |
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| CNB2008100593425A CN100559157C (en) | 2008-01-25 | 2008-01-25 | Utilize discarded ion exchange resin ball to measure the method for soil available phosphorus |
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| CN100559157C true CN100559157C (en) | 2009-11-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102890138A (en) * | 2012-09-17 | 2013-01-23 | 四川省农业科学院土壤肥料研究所 | Method for measuring readily available nutrients and reducing substances in soil of flooded rice field by utilizing ion exchange resin bag |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102128727B (en) * | 2010-12-25 | 2012-12-05 | 河南科技大学 | In-situ sampling method of phosphorus in soil |
| CN102175834B (en) * | 2011-01-24 | 2014-07-09 | 华南农业大学 | Device for stimulating nutrient absorption of plant root system and using method of device |
| CN105588745A (en) * | 2016-02-25 | 2016-05-18 | 四川省农业科学院土壤肥料研究所 | Quick detecting method for soil rapidly available nutrients in submersed paddy field |
| CN106770206B (en) * | 2017-03-15 | 2021-06-08 | 陕西省地质矿产实验研究所 | Method for measuring available phosphorus in alkaline soil by resin adsorption-assisted ICP-AES (inductively coupled plasma-atomic emission Spectrometry) method |
| CN114264528B (en) * | 2021-12-25 | 2023-10-20 | 湖北省地质局第一地质大队 | Resin treatment cylinder for detecting available phosphorus in soil and sample liquid preparation device |
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2008
- 2008-01-25 CN CNB2008100593425A patent/CN100559157C/en not_active Expired - Fee Related
Non-Patent Citations (4)
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| 应用离子交换树脂研究温度对水稻土养分释放动态的影响. 张奇春等.中国水稻科学，第17卷第4期. 2003 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102890138A (en) * | 2012-09-17 | 2013-01-23 | 四川省农业科学院土壤肥料研究所 | Method for measuring readily available nutrients and reducing substances in soil of flooded rice field by utilizing ion exchange resin bag |
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