CN100551357C - The application of prepared from coriolus versicolor mycelium in preparation resisting fatigue medicament - Google Patents
The application of prepared from coriolus versicolor mycelium in preparation resisting fatigue medicament Download PDFInfo
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Abstract
The application of prepared from coriolus versicolor mycelium in preparation resisting fatigue medicament, this medicaments preparation method is: get prepared from coriolus versicolor mycelium, adding 10 times of water gagings decocts three times, each 2 hours, collecting decoction, being evaporated to relative density at 60 ℃ is 1.04～1.10, put coldly, add 4 times of amount ethanol and make and contain alcohol amount and reach 75%, left standstill 8～12 hours at 0 ± 5 ℃, sucking filtration or the centrifugal precipitate that gets, 70 ℃ of vacuum dryings are pulverized, and cross sieve No. 3, the solid or the liquid excipient that add organic or inorganic mix, and press the medicinal preparation for oral administration of the conventional preparation method preparation of galenic pharmacy.The extract krestin of prepared from coriolus versicolor mycelium proves that through the test of pesticide effectiveness prepared from coriolus versicolor mycelium can obviously prolong big intensity endurance training rat treadmill exercise to the time that power exhausts, and has antifatigue effect.
Description
Technical field
The invention belongs to the medicinal preparation technical field of the product that contains raw material or itself and not clear structure, be specifically related to derive from the material of plant.
Background technology
Human motion to one timing, along with the prolongation of movement time, motor capacity descends gradually, sports fatigue can occur.Sports fatigue is that the physiological process of body can not continue maybe can not keep predetermined exercise intensity at a specified level.It is tired a kind of specific form that power exhausts, and is tired final stage, is meant to continue motion when fatigue, can not keep motion up to muscle or organ, and the power of being exhausts.
Sports fatigue is the combined reaction that the human body integral function changes, at present, to its mechanism still among exploring.1980, Edwards during from fatigue energy expenditure, muscular strength descend and cell excitement forfeiture three-dimensional relationship, tired catastrophe theory is proposed, and think that this is the biochemical foundation of sports fatigue, tired catastrophe theory is thought the contraction process of muscle, from the nerve to the muscle cell as chain (tired Quality Initiative): brain → spinal cord → peripheral nervous → sarolemma → transverse tubular system → Ca
2+Connection → cross-bridges anxiety → strength and power output between → actomyosin, any joint fracture all might causing motor capacity of this chain produces decline.Sports fatigue is a very complex physical phenomenon, the motion of heterogeneity, varying strength and various durations, its tired reason difference that produces.When anaerobic, a large amount of consumption of nervus centralis and decline of neuromuscular apparatus function and phosphagen are to produce tired main cause.Practice periods prolongs along with the reduction of exercise, and then lactic acid is piled up in muscle and the blood, pH value descends also becomes the reason that causes fatigue.During aerobic exercise, because the function restriction of oxygen transportation system causes the oxygen supply deficiency, and a large amount of consumption of muscle glycogen, hepatic glycogen and blood glucose is to produce tired main cause.
Sports fatigue divides central fatigue and periphery fatigue by the position that takes place, and central fatigue mainly refers to brain, has influenced cerebral activity, and periphery fatigue mainly occurs in the activation that neuromuscular point and cell membrane, calcium discharges, the contraction process position that myofilament slides.
The factor that sports fatigue produces is that sugared stockage is few in the body." depletion " opinion thinks that tired reason is that energy substance exhausts in the body.Its evidence is in the prolonged exercise, produces the same often with the reduction of blood sugar concentration of fatigue, and after the additional sugar, ability to work improves.Discoveries such as Cannon, Canis familiaris L. move to when drained, can continue again to run behind the injection epinephrine, further decompose because of epinephrine can make hepatic glycogen, thereby blood sugar level is improved.As everyone knows, glycogen is an important energy deposit material in the body, in the prolonged exercise process, the energy i (in vivo) supply, blood sugar concentration keep main decomposition by hepatic glycogen.Someone thinks that when moving for a long time, glucose because the reduction of blood glucose has influenced the work of maincenter, causes dyskinesia because the minimizing of hepatic glycogen has limited glyconeogenesis.Also the someone thinks, the minimizing of muscle glycogen content causes movement velocity to descend, and is the major reason of prolonged exercise fatigue.Now prove: when muscle glycogen reached floor level, power exhausted just and takes place, and the time that the power of reaching exhausts is directly relevant with the muscle glycogen level of motion beginning.As seen, improve before the motion glycogen stock with postpone tired take place directly related.
The factor that sports fatigue produces is that lactic acid generates more in the muscle." obstruction " opinion theory thinks that tired reason is because some metabolite accumulates in muscular tissue.Its evidence is that the lactic acid product increases in the tired muscle.Lactic acid occupies critical role in the energy supply system, it is the end-product of glycolysis energy supplying system, it is again the important oxidation matrix of aerobic metabolism energy supplying system, also may in liver, change glucose into through glyconeogenesis, but, the influence of the too much internal environment acid-base balance of lactic acid becomes negative effect again, causes tired the generation.The U.S. in 1975 research that biochemical worker blocks Ademilson of moving thinks that the lactic acid accumulation can cause the muscle ability drop, and reason is to work by the hydrion on the lactic acid molecules.Therefore, the accumulation that reduces lactic acid in the body is another factor that the delayed motion fatigue takes place, the generation and the exercise intensity of lactic acid are in close relations, the relative anoxia of motion body of big intensity of short time, the sugar anaerobic metabolism becomes the main energy supplying system of body, and therefore, lactic acid generates more in the muscle, along with the prolongation gradually of movement time, the ratio of body aerobic metabolism energy supply increases lactic acid gradually and generates also just minimizing thereupon.Long-time low-intensity motion by mobilizing the reserve capacity of oxygen transportation system, makes oxygen-supplying amount satisfy the demand of moving, so blood lactic acid does not have tangible rising.When exercise intensity was increased to 75% left and right sides of my maximal oxygen uptake, blood lactic acid obviously raise, and human body begins to mobilize the energy supply of anaerobic metabolism approach, and the bleeding from anus lactic acid concn increases progressively with the increase of exercise intensity.A large amount of studies have shown that lactic acid is piled up many more in muscle, degree of fatigue is just serious more], so, select the material that can reduce the blood lactic acid content during motion for use, can play and reduce tired effect.
The medicine that is used for the treatment of at present sports fatigue clinically has supporing yang deficiency tonifying kind Chinese patent medicine, amino acid drug, creatine, is used to increase anabolism and muscle strength.1,6 one fructose diphosphate (FDP), creatine, L-carnitine, pyruvate are arranged, be used to promote energy metabolism.Also have vitamin E, vitamin C and auxilliary sour Q 10, be used for antioxidation, regulate homeostasis.The major defect of above-mentioned Western medicine be ageing strong, side effect is big, drug dependence is strong, influence athleticly to take for a long time.The Chinese medicine regulating power is more intense, side effect is little, but it is slow to take effect, and is difficult to adapt to high-intensity training in the athletics sports.
Summary of the invention
Technical problem to be solved by this invention is to overcome the shortcoming of said medicine, for prepared from coriolus versicolor mycelium provides a kind of new purposes.
Solving the problems of the technologies described above the technical scheme that is adopted and be by prepared from coriolus versicolor mycelium is the medicinal preparation for oral administration of raw material according to following method preparation:
Get prepared from coriolus versicolor mycelium, add 10 times of water gagings and decoct each 2 hours three times, collecting decoction, being evaporated to relative density at 60 ℃ is 1.04～1.10, puts cold, adding 4 times of amount ethanol makes and contains alcohol amount and reach 75%, left standstill 8～12 hours sucking filtration or centrifugal precipitate, 70 ℃ of vacuum dryings of getting at 0 ± 5 ℃, pulverize, cross sieve No. 3, the solid or the liquid excipient that add organic or inorganic mix, and press the medicinal preparation for oral administration of the conventional preparation method preparation of galenic pharmacy.
Medicinal preparation for oral administration of the present invention is tablet, capsule, granule, oral liquid.
Coriolous Dersicolor (Fr.) Quel claims colored leather to cover bacterium again, for Basidiomycetes, Aphyllophorales, Polyporaceae, Coriolous Dersicolor (Fr.) Quel belong to.Krestin is the extract of prepared from coriolus versicolor mycelium, produce by last Haikang boat fungus polysaccharide company limited, polysaccharose substance with multiple pharmacological effect, krestin is the immunoactivator of good strengthening the body resistance, has to regulate immunity, antitumor, anti-damage, antiinflammatory, analgesia, blood fat reducing, blood sugar lowering, the impaired hepatocyte recovery of promotion and improve effect such as some Senile disease symptom.The Coriolous Dersicolor (Fr.) Quel health beverage is also developed.
The proteoglycan body that krestin is made up of 15% protein is made up of glucose, L-fucose, D-mannose, galactose and five kinds of monosaccharide of α-rhamnose.Its structure be main chain by β-1,3 glycosidic bond, side chain is that β-1,6 glycosidic bond connects.Studies show that the krestin oral toxicity is minimum, it mainly has following pharmacological action: improve learning and memory, anti-injury, antiinflammatory, analgesia, hepatoprotective, blood fat reducing, press down tumor, defying age and raise immunity etc.
The structure of krestin be main chain by β-1,3 glycosidic bond, side chain is that β-1,6 glycosidic bond connects.
Krestin is made up of glucose, L-fucose, D-mannose, galactose and five kinds of monosaccharide of α-rhamnose.From krestin, do not detect α-rhamnose but have yet, but detect the report of xylose.
Discover that the connected mode of the glycosidic bond of krestin mainly is that β (1 → 3) connects, and wherein the content of glucose surpasses 50%, therefore, thinks that krestin is is the protein-contg glucosan of connected mode with β (1 → 3).But the α connected mode that discoveries such as Zhang Ji wherein also have.Problem about the connected mode of the aminoacid kind of forming krestin and aminoacid and glycosyl yet there are no result of study report at present both at home and abroad.Research about krestin at present mainly concentrates on pharmaceutical sanitary field, and its research that replenishes direction in sports medical science especially sport nutrition does not appear in the newspapers as yet.
Effective ingredient prepares the antifatigue oral drugs with prepared from coriolus versicolor mycelium to be used with the form of conventional pharmaceutical formulation; Described conventional pharmaceutical formulation contains as active component and mixes as solid or the liquid excipient that is suitable for the organic or inorganic in the gastrointestinal with pharmaceutically acceptable carrier in preparation, this pharmaceutical formulation can be solid form such as tablet, capsule, granule, powder, pill, also can be liquid form such as Emulsion, syrup etc.
Can contain auxiliary substance, stabilizing agent, wetting agent and other additive commonly used in the above-mentioned preparation, as lactose, citric acid, tartaric acid, stearic acid, magnesium stearate, Gypsum Fibrosum powder, sucrose, corn starch, Pulvis Talci, gelatin, agar, pectin, Oleum Arachidis hypogaeae semen, olive oil, cocoa butter, ethylene glycol, ascorbic acid etc.
The preparation method of medicinal tablet of the present invention is as follows:
Get prepared from coriolus versicolor mycelium, add 10 times of water gagings and decoct three times, each 2 hours, collecting decoction, being evaporated to relative density at 60 ℃ is 1.04～1.10, puts coldly, adds 4 times of amount ethanol and makes and contain alcohol amount and reach 75%, leaves standstill 8～12 hours at 0 ± 5 ℃, sucking filtration or the centrifugal precipitate that gets, 70 ℃ of vacuum dryings are pulverized, and cross sieve No. 3, add starch, mix homogeneously adds 95% ethanol moistening, 16 mesh sieves are granulated, oven dry below 60 ℃, 14 mesh sieve granulate, tabletting, packing, promptly.Every heavy 0.4g, every contains prepared from coriolus versicolor mycelium 14.222g.
The preparation method of medicine capsule of the present invention is as follows:
Prepared from coriolus versicolor mycelium raw material that medicine capsule of the present invention is used and weight proportion and medicinal tablet of the present invention are identical, the extraction process step of prepared from coriolus versicolor mycelium is identical with the extraction process step of method for preparing tablet thereof prepared from coriolus versicolor mycelium of the present invention, and used adjuvant and other processing step are undertaken by the conventional preparation method of capsule.Every heavy 0.3g, every contains prepared from coriolus versicolor mycelium 10.667g.
The preparation method of medicinal granule of the present invention is as follows:
The used prepared from coriolus versicolor mycelium raw material of prepared from coriolus versicolor mycelium raw material that medicinal granule of the present invention is used and weight proportion and medicinal tablet of the present invention is identical, the extraction process step of prepared from coriolus versicolor mycelium is identical with the extraction process step of method for preparing tablet thereof prepared from coriolus versicolor mycelium of the present invention, and used adjuvant and other processing step are undertaken by the conventional preparation method of granule.Every bag heavy 5g, every gram contains prepared from coriolus versicolor mycelium 8.533g.
The preparation technology of medicine oral liquid of the present invention is as follows:
Prepared from coriolus versicolor mycelium raw material that medicine oral liquid of the present invention is used and weight proportion and medicinal tablet of the present invention are identical, the extraction process step of prepared from coriolus versicolor mycelium is identical with the extraction process step of method for preparing tablet thereof prepared from coriolus versicolor mycelium of the present invention, and used adjuvant and other processing step carry out according to the conventional preparation method of oral liquid.Every bottle of 10mL, every milliliter contains prepared from coriolus versicolor mycelium 4.267g.
The effective ingredient prepared from coriolus versicolor mycelium is as the antifatigue oral drugs, and the oral drugs of the various dosage forms of prepared one-tenth become human oral, and prepared from coriolus versicolor mycelium content is 128g every day in the medicine
The extract krestin of the crude drug prepared from coriolus versicolor mycelium of medicine of the present invention proves that through the test of pesticide effectiveness prepared from coriolus versicolor mycelium can obviously prolong big intensity endurance training rat treadmill exercise to the time that power exhausts, and has antifatigue effect.
The specific embodiment
Send out below that the present invention is described in more detail in conjunction with the embodiments, but the invention is not restricted to these embodiment.
Embodiment 1
With 1000 of production tablet products of the present invention is that the used medicinal raw material of example and adjuvant and weight proportion thereof are:
Prepared from coriolus versicolor mycelium 14222g
Starch adds to 400g.
Get prepared from coriolus versicolor mycelium 14222g, add 10 times of water gagings and decoct three times, each 2 hours, collecting decoction, being evaporated to relative density at 60 ℃ is 1.04～1.10, puts coldly, adds 4 times of amount ethanol and makes and contain alcohol amount and reach 75%, leaves standstill 8～12 hours at 0 ± 5 ℃, sucking filtration or the centrifugal precipitate that gets, 70 ℃ of vacuum dryings are pulverized, cross sieve No. 3, add starch to 400g, mix homogeneously, add 95% ethanol moistening, 16 mesh sieves and granulate, oven dry below 60 ℃, 14 mesh sieve granulate, tabletting, packing, promptly.Every heavy 0.4g, every contains prepared from coriolus versicolor mycelium 14.222g.
Embodiment 2
With 1000 of production capsule products of the present invention is that the used medicinal raw material of example and adjuvant and weight proportion thereof are:
Prepared from coriolus versicolor mycelium 10667g
Starch adds to 300g.
Its preparation method is undertaken by the preparation method of capsule of the present invention.Every heavy 0.3g, every contains prepared from coriolus versicolor mycelium 10.667g.
Embodiment 3
With production granule product of the present invention 1000 grams is that the used medicinal raw material of example and adjuvant and weight proportion thereof are:
Prepared from coriolus versicolor mycelium 8533g
Sucrose 400g
Dextrin adds to 1000g.
Its preparation method is undertaken by the preparation technology of granule of the present invention.Every bag heavy 5g, every gram contains prepared from coriolus versicolor mycelium 8.533g.
Embodiment 4
With production oral liquid product of the present invention 1000mL is that the used medicinal raw material of example and adjuvant and weight proportion thereof are:
Prepared from coriolus versicolor mycelium 4267g
Sucrose 400g
Distilled water adds to 1000mL.
Its preparation method is undertaken by oral liquor of the present invention.Every bottle of 10mL, every milliliter contains prepared from coriolus versicolor mycelium 4.267g.
In order to verify the effect of medicine of the present invention to the fatigue treatment, the inventor adopts the extract krestin of the crude drug prepared from coriolus versicolor mycelium of medicine of the present invention to carry out the test of pesticide effectiveness, and various test situation are as follows:
Test objective: adopt the whole animal test, observe the antifatigue effect of medicine of the present invention, the effect of reflection medicine of the present invention with cure mainly, for clinical trial provides theoretical foundation.
Be subjected to the reagent thing: the extract krestin of prepared from coriolus versicolor mycelium, produce by last Haikang boat fungus polysaccharide company limited.
Experiment reagent: protein quantification (biuret) test kit, bio-engineering research institute is built up in Nanjing; Superoxide dismutase (SOD) testing cassete, bio-engineering research institute is built up in Nanjing; Malonaldehyde (MDA) testing cassete, bio-engineering research institute is built up in Nanjing; Total antioxidant capacity (T-AOC) testing cassete, bio-engineering research institute is built up in Nanjing; Glutathion peroxidase (GSH-Px) testing cassete, bio-engineering research institute is built up in Nanjing; Catalase (CAT) testing cassete, bio-engineering research institute is built up in Nanjing; Nitric oxide (NO) testing cassete, bio-engineering research institute is built up in Nanjing; Nitric oxide synthetase (NOS) testing cassete, bio-engineering research institute is built up in Nanjing; Lactic acid dehydrogenase (LDH) testing cassete, bio-engineering research institute is built up in Nanjing; Muscle glycogen and hepatic glycogen test kit, Nanjing build up bio-engineering research institute; Coomassie brilliant blue protein reagent box, Nanjing build up bio-engineering research institute; Creatinine assay test kit, Nanjing build up bio-engineering research institute; Aspartate amino transferase reagent, Nanjing build up bio-engineering research institute; Alanine aminotransferase test kit, Nanjing build up bio-engineering research institute; Creatine kinase test kit, Nanjing build up bio-engineering research institute; Blood sugar detection test kit, Nanjing build up bio-engineering research institute; Ferric sesquichloride hemoglobin standard liquid, bio-engineering research institute is built up in Nanjing; Hemoglobinometry test kit, Nanjing build up bio-engineering research institute; Anticoagulant heparin, Nanjing build up bio-engineering research institute; Sodium carboxymethyl cellulose 0.3%, self-control; Dehydrated alcohol (AR), Xi'an chemical reagent factory; Ether (AR), Shanghai chemical reagents corporation of Chinese Medicine group; Glacial acetic acid (AR), Tianjin chemical reagent three factories.
Experiment equipment: electronic treadmill, Chinese Hang Zhouduanshi makes; The desk-top high-band refrigerated centrifuger of TGL-16G, Anting Scientific Instrument Factory, Shanghai; 721B type spectrophotometer, Shanghai the 3rd analytical tool factory; 751B type ultraviolet spectrophotometer, Shanghai the 3rd analytical tool factory; DK-98-1A constant temperature bath, safe this peculiar limit company in Tianjin; BCD-203A appearance sound refrigerator, dragon electrical equipment limited company of Chinese section; The TN-100 table pan torsion bal, Wuhan instrument and meter for automation factory; The onso-TCS-2000A electronic scale, Wuhan instrument and meter for automation factory; R-200D type electronic scale, Germany; The two circle version of MP-200-1 type electronic scale, Shanghai Second Balance Factory.
Laboratory animal: 32 of male and healthy rats, body weight 170-220g is provided by Xi'an Jiaotong University Medical College's Experimental Animal Center.The dry forage feed of national standard rodent, free diet, 23 ℃ ± 5 ℃ of animal housing's temperature, relative humidity 40%-70%, sub-cage rearing is standby.
1, sets up experimental animal model
After the laboratory animal adaptability was raised 7d, with 15m/min, the 5min/d quantity of motion scheduled to last the screening of 3d, superseded indivedual incompatibility treadmill training persons to animal.To remain rat and be divided into 4 groups at random: 8 of quiet matched groups, 8 of quiet dosing groups, motoricity exhaust 8 of matched groups, motoricity exhausts 8 of dosing groups.Quiet matched group and quiet dosing group are raised under rest state, and motoricity is exhausted matched group and motoricity exhaust the dosing group and move, (W) six times weekly, motion scheme sees Table 1.Carry out power when training the last time and exhaust motion, power exhausts criterion and is, animal does not catch up with predetermined speed, and the rat buttocks is pressed in cage tool rear wall, and hind leg drags after with rotating belt and reaches 30 seconds, hairbrush stimulate drive invalid.Behavior characteristics is anxious deeply for breathing, amplitude is big, and look tired, and hanging one's head in the ventricumbent position, stimulates the back reactionless.
Do with the speed of 15m/min before the every day training and adapt to formal training behind the motion 5min, training pattern is adjusted in conjunction with reality slightly based on the Bedford model, 8 weeks of training period.Concrete experimental program sees Table 1.
Table 1 laboratory animal motion scheme
2, medication
Quiet matched group: the sodium carboxymethyl cellulose normal saline solution 2ml gastric infusion with 0.3%, once a day, totally 42 days.
The motion matched group: the sodium carboxymethyl cellulose reason saline solution 2ml gastric infusion with 0.3%, once a day, totally 42 days.
Motion administration group: it is that dosing group dosage is 140mg/kg/d in 0.3% the sodium carboxymethyl cellulose normal saline solution that krestin is dissolved in 2ml concentration, once a day, and totally 42 days.
3, preparation experiment specimen
The 8th last 1 day of week, quiet matched group and quiet dosing group are weighed under rest state, motion matched group and motion dosing group exhaust outside motion back record power exhausts the time, weighs in power, each group etherization, broken end is got blood, after getting normal saline that brain, kidney, liver, the heart and quadriceps femoris place pre-cooling rapidly and cleaning blood stains, blot with filter paper and to be placed on-20 ℃ of refrigerators and to preserve standby.
Preparation serum: get in the rearmounted 37 ℃ of water-baths of blood after 30 minutes, then with 2500～3000 rev/mins centrifugal 10 minutes, to extract supernatant be serum and preserve standby in 4 ℃ of refrigerators.
The preparation tissue homogenate: taking by weighing 0.2～1g tissue is that 1/9 ratio adds the homogenate medium (pH7.4 that gets pre-cooling in W (g) piece of tissue weight/V (ml) homogenate medium, 0.01mol/L Tris-HCL, 0.0001mol/L disodiumedetate [EDTA-2Na], 0.01mol/L sucrose, 0.8% NaCL solution) in beaker, cut for a short time with ophthalmology and to shred piece of tissue (above all operating in the ice-water bath carried out) as early as possible.After the manual preparation homogenate, 3000 rev/mins of low-temperature centrifugations 10～15 minutes, 4 ℃ of refrigerator cold-storages of separation and Extraction supernatant or-20 ℃ of refrigerators are freezing standby, discard following precipitation.
Statistical procedures: carry out date processing by the SPSS statistical software, (X ± s), carry out the t check determines the significance of difference to conventional method computation of mean values ± standard deviation.
4, experimental result
(1) prepared from coriolus versicolor mycelium is to the influence of experimental rat body weight and some organ index
Experimental technique: weigh before laboratory animal is put to death, core immediately after the execution, liver, spleen, thymic tissue, take out the back and clean, and weigh after blotting with filter paper with normal saline.
Test result sees Table 2.
Table 2 prepared from coriolus versicolor mycelium is to the influence of experimental rat body weight and some organ index
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
Table 2 result shows, quiet dosing group rat and motion dosing group rat body weight all are lower than its matched group slightly, but there was no significant difference, feel concerned about number, liver index, spleen index and the thymus index of quiet dosing group all are higher than quiet matched group, but have only liver index differential significantly (P＜0.05); Each organ index of motion matched group is compared no significant difference with quiet matched group; Each organ index of motion dosing group is all than motion matched group height, but has only liver index differential significantly (P＜0.05).
(2) prepared from coriolus versicolor mycelium exhausts the influence of time to treadmill exercise rat power
Experimental technique: the 8th last 1 day of the week of taking medicine to rat, motion matched group and motion dosing group rat are placed respectively on the animal treadmill, carrying out disposable power exhausts test and carries out the test that power exhausts time index, power exhausts criterion, animal does not catch up with predetermined speed, the rat buttocks is pressed in cage tool rear wall, and hind leg drags after with rotating belt and reaches 30 seconds, hairbrush stimulate drive invalid.Behavior characteristics is anxious deeply for breathing, amplitude is big, and look tired, and hanging one's head in the ventricumbent position, stimulates the back reactionless.
Test result sees Table 3.
Table 3 prepared from coriolus versicolor mycelium exhausts the influence of time to treadmill training rat power
Annotate:
▲Expression compares with the training group that there were significant differences (P＜0.05)
Table 3 result shows, prepared from coriolus versicolor mycelium can significant prolongation rat treadmill exercise to the time that power exhausts, compare with the motion matched group, motion dosing group power has exhausted time lengthening 24.57%.
(3) prepared from coriolus versicolor mycelium is to the influence of big some serum index of intensity endurance exercise rat
Experimental technique: get respectively that whole blood 0.05ml and serum 1ml carry out alanine aminotransferase (ALT), aspartate amino transferase (AST), lactic acid dehydrogenase (LDH), creatine kinase (CK), BUN (blood urea nitrogen), (test of creatinine, Bla (blood lactic acid) index, method of testing is carried out according to the operational approach of alanine aminotransferase, aspartate amino transferase, lactic acid dehydrogenase, creatine kinase, blood urea nitrogen, hemoglobin, creatinine, blood lactic acid test kit respectively for Hb (hemoglobin), Cr.Test result sees Table 4.
The influence of table 4 prepared from coriolus versicolor mycelium active and serum urea nitrogen, hemoglobin, creatinine, blood lactic acid content to some sero-enzyme of treadmill training rat
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
Table 4 result shows, compares with quiet matched group, and quiet dosing group rat serum hemoglobin and creatinine content significantly or extremely significantly raise (P＜0.05 or P＜0.01), and other numerical value then do not have significant difference; The motion matched group is compared serum alanine aminotransferase, aspartate amino transferase, lactic acid dehydrogenase, creatine kinase activity and blood urea nitrogen, blood lactic acid content all significantly or extremely significantly rise (P＜0.05 or P＜0.01) with quiet matched group, content of hemoglobin significantly reduces (P＜0.05), and creatinine content has reduction trend but relatively do not have significant difference; Compare with the motion matched group, motion dosing group rat blood serum alanine aminotransferase, aspartate amino transferase, creatine kinase, lactic acid dehydrogenase activity and blood urea nitrogen, blood lactic acid content all significantly reduce (P＜0.05), hemoglobin and creatinine content significantly raise (P＜0.05).
(4) prepared from coriolus versicolor mycelium is to the influence of big intensity endurance training rat blood sugar, hepatic glycogen and muscle glycogen content
Experimental technique: get the test that serum 0.2ml, 0.37g quadriceps femoris and 0.75g hepatic tissue carry out blood glucose and flesh hepatic glycogen index respectively, method of testing is carried out according to the operational approach of blood glucose and hepatic glycogen test kit respectively.
Test result sees Table 5.
Table 5 prepared from coriolus versicolor mycelium is to the influence of big intensity endurance training rat blood sugar, hepatic glycogen and muscle glycogen content
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
As can be seen from Table 5, blood glucose of quiet dosing group rat and hepatic glycogen content all are significantly higher than quiet matched group (P＜0.05), and the muscle glycogen content of quiet dosing group rat is compared on the rise with quiet matched group, but difference is not remarkable; Power exhausts motion control rats blood glucose, and the hepatic glycogen level is compared with quiet matched group, all has significantly or the reduction (P＜0.05 or P＜0.01) of utmost point significance; Blood glucose, hepatic glycogen and the muscle glycogen level that power exhausts dosing group rat all significantly or the utmost point power of being significantly higher than exhaust exercise group (P＜0.05 or P＜0.01).
(5) prepared from coriolus versicolor mycelium is organized the influence of Radical Metabolism to big intensity endurance training rat heart muscle
Experimental technique: get the test that serum 1ml carries out serum Radical Metabolism index, method of testing is carried out according to the operational approach of Total antioxidant capacity (T-AOC), malonaldehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) test kit respectively.
Test result sees Table 6.
Table 6 prepared from coriolus versicolor mycelium is organized the influence of Radical Metabolism to big intensity endurance training rat heart muscle
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
As can be seen from Table 6, Total antioxidant capacity, superoxide dismutase and glutathion peroxidase all are significantly higher than quiet matched group (P＜0.05) in the quiet dosing group rat heart muscle tissue, catalase has been compared rising trend but there was no significant difference with quiet matched group, and malonaldehyde is compared remarkable reduction (P＜0.05) with quiet matched group; Total antioxidant capacity, catalase and glutathion peroxidase are lower than quiet matched group in the motion matched group cardiac muscular tissue, data difference has significantly or extremely remarkable meaning (P＜0.05 or P＜0.01), and mda content and superoxide dismutase activity are remarkable or the utmost point is significantly higher than quiet matched group (P＜0.05 or P＜0.01); Motion dosing group rat heart muscle and motoricity exhaust group to be compared Total antioxidant capacity, catalase, superoxide dismutase and glutathion peroxidase and significantly raises (P＜0.05), and the malonaldehyde level significantly reduces (P＜0.05).
(6) prepared from coriolus versicolor mycelium is to the influence of big intensity endurance training liver tissues of rats Radical Metabolism
Experimental technique: get the test that the 1ml of liver tissue homogenate carries out hepatic tissue Radical Metabolism index, method of testing is carried out according to the operational approach of Total antioxidant capacity (T-AOC), malonaldehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) test kit respectively.
Test result sees Table 7.
Table 7 prepared from coriolus versicolor mycelium is to the influence of big intensity endurance training liver tissues of rats Radical Metabolism
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
As can be seen from Table 7, Total antioxidant capacity, superoxide dismutase and glutathion peroxidase all are significantly higher than quiet matched group (P＜0.05) in the quiet dosing group liver tissues of rats, catalase has been compared rising trend but there was no significant difference with quiet matched group, and malonaldehyde is compared remarkable reduction (P＜0.05) with quiet matched group; Total antioxidant capacity, catalase and glutathion peroxidase are lower than quiet matched group in the training group hepatic tissue, data difference has significantly or extremely remarkable meaning (P＜0.05 or P＜0.01), and mda content and superoxide dismutase activity all the utmost point be significantly higher than quiet matched group (P＜0.01); Motion dosing group liver tissues of rats and motoricity exhaust group to be compared Total antioxidant capacity, catalase, superoxide dismutase and glutathion peroxidase and significantly raises (P＜0.05), and the malonaldehyde level significantly reduces (P＜0.05).
(7) prepared from coriolus versicolor mycelium is organized the influence of Radical Metabolism to big intensity endurance training rat quadriceps femoris
Experimental technique: get quadriceps femoris tissue homogenate 1ml and carry out the test that quadriceps femoris is organized the Radical Metabolism index, method of testing is carried out according to the operational approach of Total antioxidant capacity (T-AOC), malonaldehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) test kit respectively.
Test result sees Table 8.
Table 8 prepared from coriolus versicolor mycelium is organized the influence of Radical Metabolism to big intensity endurance training rat quadriceps femoris
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
As can be seen from Table 8, Total antioxidant capacity, superoxide dismutase and catalase all are significantly higher than quiet matched group (P＜0.05) in the quiet dosing group rat quadriceps femoris tissue, glutathion peroxidase has been compared rising trend but there was no significant difference with quiet matched group, and malonaldehyde is compared remarkable reduction (P＜0.05) with quiet matched group; Catalase and glutathion peroxidase are lower than quiet matched group in the training group quadriceps femoris tissue, data difference has significantly or extremely remarkable meaning (P＜0.05 or P＜0.01), and mda content and superoxide dismutase activity are remarkable or the utmost point is significantly higher than quiet matched group (P＜0.05 or P＜0.01); Motion dosing group rat quadriceps femoris tissue and motoricity exhaust group to be compared Total antioxidant capacity, catalase, superoxide dismutase and glutathion peroxidase and significantly or extremely significantly raises (P＜0.05 or P＜0.01), and the malonaldehyde level significantly reduces (P＜0.05).
(8) prepared from coriolus versicolor mycelium is organized the influence of Radical Metabolism to big intensity endurance training kidney of rats
Experimental technique: get the test that kidney homogenate 1ml carries out nephridial tissue Radical Metabolism index, method of testing is carried out according to the operational approach of Total antioxidant capacity (T-AOC), malonaldehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) test kit respectively.
Test result sees Table 2.
Table 9 prepared from coriolus versicolor mycelium is organized the influence of Radical Metabolism to big intensity endurance training kidney of rats
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
As can be seen from Table 9, superoxide dismutase and catalase all are significantly higher than quiet matched group (P＜0.05) in the quiet dosing group kidney of rats tissue, glutathion peroxidase has been compared rising trend but there was no significant difference with Total antioxidant capacity with quiet matched group, and malonaldehyde is compared remarkable reduction (P＜0.05) with quiet matched group; Total antioxidant capacity, catalase, superoxide dismutase and glutathion peroxidase are lower than quiet matched group in the training group nephridial tissue, data difference has significantly or extremely remarkable meaning (P＜0.05 or P＜0.01), and mda content is significantly higher than quiet matched group (P＜0.05); Motion dosing group kidney of rats tissue exhausts group with motoricity and compares all significantly risings (P＜0.05) of Total antioxidant capacity, catalase, superoxide dismutase and glutathion peroxidase, and the malonaldehyde level significantly reduces (P＜0.05).
(9) prepared from coriolus versicolor mycelium is to the influence of big intensity endurance training rat cerebral tissue Radical Metabolism
Experimental technique: get the test that the 1ml of brain tissue homogenate carries out cerebral tissue Radical Metabolism index, method of testing is carried out according to the operational approach of Total antioxidant capacity (T-AOC), malonaldehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) test kit respectively.
Test result sees Table 10.
Table 10 prepared from coriolus versicolor mycelium is to the influence of big intensity endurance training rat cerebral tissue Radical Metabolism
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
As can be seen from Table 10, Total antioxidant capacity, superoxide dismutase, catalase and glutathion peroxidase all are significantly higher than quiet matched group (P＜0.05) in the peace and quiet dosing group rat cerebral tissue, and malonaldehyde is compared remarkable reduction (P＜0.05) with quiet matched group; Total antioxidant capacity, catalase, superoxide dismutase and glutathion peroxidase are lower than quiet matched group in the training group cerebral tissue, data difference has significantly or extremely remarkable meaning (P＜0.05 or P＜0.01), and mda content is significantly higher than quiet matched group (P＜0.05); Motion dosing group rat cerebral tissue and motoricity exhaust group and compare Total antioxidant capacity, superoxide dismutase and glutathion peroxidase significantly raise (P＜0.05), catalase is higher than the training group but difference is not remarkable, and the malonaldehyde level significantly reduces (P＜0.05).
(10) prepared from coriolus versicolor mycelium is to the active influence of the big total nitric oxide synthetase of intensity endurance training rat portion of tissue
Experimental technique: get liver tissue homogenate, cardiac muscular tissue's homogenate, quadriceps femoris tissue homogenate, brain tissue homogenate, kidney homogenate 0.1ml respectively and respectively organize the active test of total nitric oxide synthetase, method of testing is carried out according to the operational approach of nitric oxide synthetase test kit.
Test result sees Table 11.
Table 11 prepared from coriolus versicolor mycelium is to the active influence of the big total nitric oxide synthetase of intensity endurance training rat tissue (unit: U/mg prot)
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
As can be seen from Table 11, power exhausts the quieter matched group height of total nitric oxide synthetase activity in motion control rats liver, kidney, the quadriceps femoris tissue, have remarkable or utmost point significant difference (P＜0.05 or P＜0.01), and total quieter matched group height of nitric oxide synthetase activity but difference is not remarkable in the cardiac muscle, total nitric oxide synthetase activity in the cerebral tissue is lower than quiet matched group, and difference is not remarkable; Power exhausts in liver, cardiac muscle, quadriceps femoris and the cerebral tissue of motion dosing group total nitric oxide synthetase activity and is higher than power and exhausts exercise group, and there were significant differences (P＜0.05), and total nitric oxide synthetase activity exhausts matched group with power and compares no significant difference in the nephridial tissue.
(11) prepared from coriolus versicolor mycelium is to the active influence of big intensity endurance training rat portion of tissue inducible nitric oxide synthase
Experimental technique: get liver tissue homogenate, cardiac muscular tissue's homogenate, quadriceps femoris tissue homogenate, brain tissue homogenate, kidney homogenate 0.1ml respectively and respectively organize the active test of inducible nitric oxide synthase, method of testing is carried out according to the operational approach of nitric oxide synthetase test kit.
Test result sees Table 12.
Table 12 prepared from coriolus versicolor mycelium is to the active influence of big intensity endurance training rat tissue's inducible nitric oxide synthase (unit: U/mg prot)
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
As can be seen from Table 12, power exhaust inducible nitric oxide synthase in control rats liver, cardiac muscle, quadriceps femoris, the nephridial tissue active significantly or the utmost point be significantly higher than quiet matched group (P＜0.05 or P＜0.01), quieter matched group height in the cerebral tissue but difference is not remarkable; Power exhausts in dosing group rats'liver, cardiac muscle, the quadriceps femoris inducible nitric oxide synthase activity, and to exhaust matched group than power low, has significantly or utmost point significant difference (P＜0.05 or P＜0.01).To exhaust matched group than power low for the inducible nitric oxide synthase activity in the nephridial tissue, but difference is not remarkable.The inducible nitric oxide synthase activity power of being significantly higher than exhausts matched group level (P＜0.05) in the cerebral tissue.
(12) prepared from coriolus versicolor mycelium is to big intensity endurance training rat portion of tissue effect of nitric oxide
Experimental technique: get the test that liver tissue homogenate, cardiac muscular tissue's homogenate, quadriceps femoris tissue homogenate, brain tissue homogenate, kidney homogenate 0.1ml respectively organize content of nitric oxide respectively, method of testing is carried out according to the operational approach of nitric oxide test kit.
Test result sees Table 13.
Table 13 prepared from coriolus versicolor mycelium is to effect of nitric oxide (unit: μ mol/g prot) of big intensity endurance training rat tissue
Annotate: ★ represents to compare with quiet matched group ★ P＜0.05 ★ ★ P＜0.05
△ represents to compare with the motion matched group △ P＜0.05 △ △ P＜0.05
As can be seen from Table 13, power exhausts that content of nitric oxide is higher than quiet matched group in matched group liver, quadriceps femoris, the nephridial tissue, but has only nephridial tissue significant difference (P＜0.05), and myocardium content of nitric oxide is higher than quiet matched group, but difference is not remarkable.Low and the quiet matched group of content of nitric oxide in the brain, but difference is not remarkable; Power exhaust in dosing group rats'liver, cardiac muscle, quadriceps femoris, the cerebral tissue content of nitric oxide all the power of being significantly higher than exhaust matched group level (P＜0.05), content of nitric oxide exhausts matched group with power and compares no significant difference in the nephridial tissue.
5, experiment conclusion
(1) prepared from coriolus versicolor mycelium can obviously prolong big intensity endurance training rat treadmill exercise to the time that power exhausts, and has antifatigue effect.
(2) prepared from coriolus versicolor mycelium can improve the organ index of rat, improves their function, also might strengthen the immunocompetence of rat, simultaneously rat growthing development is had no adverse effects.
(3) prepared from coriolus versicolor mycelium can significantly reduce the increase that training and power exhaust rat blood serum alanine aminotransferase that motion causes, aspartate amino transferase, lactic acid dehydrogenase, creatine kinase activity, and prompting prompting prepared from coriolus versicolor mycelium can alleviate power and exhaust the damage of motion to tissues such as rat liver, cardiac muscles.
(4) prepared from coriolus versicolor mycelium can significantly raise and descend because of power exhausts the content of hemoglobin that causes that moves, significantly reduction power exhausts the rising that motion causes blood urea nitrogen, blood lactic acid content, make creatinine content rising in the rat serum, the prompting prepared from coriolus versicolor mycelium can improve aerobic sport ability, reduce motion rat body internal protein decomposition rate, increase creatine and phosphagen content in the muscle, improve muscle quality.
(5) prepared from coriolus versicolor mycelium can increase sugar deposit in the rat body, guarantees that the energy of tissues such as central nervous system, motor and erythrocyte is supplied with, and delays the generation of maincenter and periphery fatigue, thereby improves motor capacity.
(6) prepared from coriolus versicolor mycelium can significantly reduce MDA content in the big intensity endurance training rat heart, liver, flesh, brain, the kidney, improve the activity of enzymes such as superoxide dismutase, glutathion peroxidase, catalase, improve training rat body Total antioxidant capacity, thereby to the tangible retarding action that has of the kinetic exercise fatigue that causes because of the excessive generation of free radical.
(7) prepared from coriolus versicolor mycelium can improve the total nitric oxide synthetase activity of rat body, appropriateness improves rat tissue's content of nitric oxide, tissue blood flow's amounts such as skeletal muscle, cardiac muscle when improving motion effectively guarantee oxygen and the supply of other nutrient substance and taking out of of metabolite.
Function of the present invention: eliminate free radical, improve immunocompetence, anti-cardiac muscle and cerebral hypoxia ischemia, anti-fatigue effect.
Specification of the present invention: every heavy 0.3g of medicine capsule of the present invention, every contains prepared from coriolus versicolor mycelium 10.667g; Every heavy 0.4g of medicinal tablet of the present invention, every contains prepared from coriolus versicolor mycelium 14.222g; Every bag heavy 5g of medicinal granule of the present invention, every gram contains prepared from coriolus versicolor mycelium 8.533g; Every bottle of 10mL of medicine oral liquid of the present invention, every milliliter contains prepared from coriolus versicolor mycelium 4.267g.
Usage and dosage of the present invention: oral three times of every day, 1 bottle of 4 of each oral capsules or 3 in tablet or 1 bag of granule or oral liquid.
Claims (2)
1, the application of prepared from coriolus versicolor mycelium in preparation resisting fatigue medicament, this medicament prepares as follows:
Get prepared from coriolus versicolor mycelium, add 10 times of water gagings and decoct each 2 hours three times, collecting decoction, being evaporated to relative density at 60 ℃ is 1.04～1.10, puts cold, adding 4 times of amount ethanol makes and contains alcohol amount and reach 75%, left standstill 8～12 hours sucking filtration or centrifugal precipitate, 70 ℃ of vacuum dryings of getting at 0 ± 5 ℃, pulverize, cross sieve No. 3, the solid or the liquid excipient that add organic or inorganic mix, and press the medicinal preparation for oral administration of the conventional preparation method preparation of galenic pharmacy.
2, according to the application of the described prepared from coriolus versicolor mycelium of claim 1 in preparation resisting fatigue medicament, it is characterized in that: said medicinal preparation for oral administration is tablet, capsule, granule, oral liquid.
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Non-Patent Citations (8)
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| 不同云芝菌株及提取工艺所得结合蛋白多糖的分析比较. 杨晓彤，糜可，冯慧琴，吴予尘，杨庆尧.中国医药工业杂志，第31卷第12期. 2000 |
| 不同云芝菌株及提取工艺所得结合蛋白多糖的分析比较. 杨晓彤，糜可，冯慧琴，吴予尘，杨庆尧.中国医药工业杂志，第31卷第12期. 2000 * |
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Assignee: Sirio Pharma Co., Ltd. Assignor: Shaanxi Normal University Contract fulfillment period: 2009.11.23 to 2014.12.23 contract change Contract record no.: 2010440000134 Denomination of invention: Anti-fatigue medicine prepared from coriolus versicolor mycelium Granted publication date: 20091021 License type: Exclusive license Record date: 2010.1.6 |
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