CN100546591C - A kind of Gekko Swinhonis Radix Actinidiae Chinensis water extract that is used for the treatment of hepatocarcinoma - Google Patents
A kind of Gekko Swinhonis Radix Actinidiae Chinensis water extract that is used for the treatment of hepatocarcinoma Download PDFInfo
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- CN100546591C CN100546591C CNB2006100669938A CN200610066993A CN100546591C CN 100546591 C CN100546591 C CN 100546591C CN B2006100669938 A CNB2006100669938 A CN B2006100669938A CN 200610066993 A CN200610066993 A CN 200610066993A CN 100546591 C CN100546591 C CN 100546591C
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- actinidiae chinensis
- radix actinidiae
- gekko swinhonis
- water extract
- hepatocarcinoma
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Abstract
The present invention relates to a kind of water extract that is used for the treatment of the Gekko Swinhonis Radix Actinidiae Chinensis of hepatocarcinoma, rice is fried back Gekko Swinhonis dry powder body and Radix Actinidiae Chinensis carry out water extraction together and obtain.With this Gekko Swinhonis Radix Actinidiae Chinensis water extract is effective ingredient, can have the inhibition effect to hepatoma cell strain Bel-7402, and suppression ratio can reach 60% under low concentration, shows that it can be used to prepare the medicine for the treatment of hepatocarcinoma.The present invention has widened the medical applications field of this compound recipe of Gekko Swinhonis Radix Actinidiae Chinensis.
Description
Technical field
The invention belongs to the field of Chinese medicines, specifically relate to a kind of water extract that is used for the treatment of the Gekko Swinhonis Radix Actinidiae Chinensis of hepatocarcinoma.
Background technology
The Invasion and Metastasis of cancer is cancer patient's main causes of death, seeks hypotoxic natural drug and has great importance for clinical practice as tumor cell proliferation, Invasion and Metastasis inhibitor and natural death of cerebral cells derivant.At China's many single, proved recipes that are being scattered among the people, these prescriptions are often having good effect aspect the treatment cancer, and the embodiment of Chinese medicine marrow is also just aspect herbal mixture.House liyard powder and Radix Actinidiae Chinensis are two kinds of relatively common Chinese herbal medicine, are eliminating evil medicine.
According to the Compendium of Material Medica record, Gekko Swinhonis has counteracting toxic substances and treats skin ulcer, effects such as tonifying the lung kidney, foster essence and blood, relieving cough and asthma, expelling wind for relieving convulsion, dispelling toxins and dredging collaterals, eliminating stagnation.In the nearly more than ten years, Gekko Swinhonis is widely used in the multiple disease of clinical treatment, particularly when being used for multiple difficult miscellaneous diseases such as various malignant tumor, tuberculosis, myelopathy, fistula disease, sinus tract and surgery infection, definite curative effect (Xie Shuan etc. are all arranged, bright Gekko Swinhonis extract suppresses the increment of C6 glioma cell and induces and transfer the research of dying, treatment and prevention of tumour research, 2003,30 (6): 458-461; Chen Ming etc., Chinese medicine Gekko Swinhonis modern study progress, one modernization of Chinese medicine of World Science technology, 2001,3 (4): 53-56; Song Hongen etc., magnificent brave inside fire attack soup and hot compress Xiaoaisan powder for curing cancer treatment primary lung cancer 118 examples, the Jiangsu traditional Chinese medical science, 1996,17 (7): 22-23; Wu Benduan, compound recipe house liyard powder cooperate the radiotherapy in the treatment advanced esophageal carcinoma to observe practical Chinese medicine magazine, 1999,15 (10): 16-17).
Radix Actinidiae Chinensis is the root of Actinidiaceae plant Fructus actinidiae chinensis (Actinidia chinensis Planch) and Actinidia arguta Sieb.et Zucc (A.arguta Planch), beginning is stated from " Kaibao Bencao ", its Herb is rich in actinidine, root contains ursolic acid, oleanolic acid, succinic acid, daucosterol and arginine, lysine, leucine, alanine, aspartic acids etc. also are rich in Fe, Cu, Mn, elemental compositions such as Se, have and be good for the stomach, heat clearing away, effect (the Wei Jinyu etc. of dampness removing, in the experimentation of Radix Actinidiae Chinensis extract antitumor action more, the Guangxi Chinese medicine, 2003,26 (6): 49; Yao Qinghua etc., compound recipe Radix Actinidiae Chinensis preparation raises the research of PG GJIC function, tumor research and clinical, 2004,10 (16): 303-305; Zhong Zhenguo etc., in the more anti tumor activity in vitro research of Radix Actinidiae Chinensis extract, Chinese crude drug, 2005,28 (3): 215-218).
Wenzhou folklore boils the compound treatment gastric cancer of Fructus Jujubae with house liyard powder, Radix Actinidiae Chinensis.But do not see the report of this compound recipe for other tumor.
Summary of the invention
The object of the present invention is to provide a kind of water extract that is used for the treatment of the Gekko Swinhonis Radix Actinidiae Chinensis of hepatocarcinoma.
The objective of the invention is to realize by the following technical solutions:
The water extract that is used for the treatment of the Gekko Swinhonis Radix Actinidiae Chinensis of hepatocarcinoma provided by the invention is fried back Gekko Swinhonis dry powder body and Radix Actinidiae Chinensis with rice and is carried out water extraction together and obtain, and its concrete preparation method comprises the steps:
1) rice is fried the preparation of house liyard powder:
With commercially available house liyard powder and rice according to weight ratio 3～6: 1 mix homogeneously, put into marmite then and stir-fry, until the rice buff, remove rice, take out the house liyard powder cold of drying in the air;
2) preparation of house liyard powder and Radix Actinidiae Chinensis water extract:
After the house liyard powder of step 1) and Radix Actinidiae Chinensis mixed by weight 1: 3～9, add distilled water, reflux is carried out lixiviate, obtains the aqueous extract of Gekko Swinhonis Radix Actinidiae Chinensis; Remove at 30～45 ℃ of rotary evaporations then and desolvate, until obtaining brown paste, vacuum drying 24h obtains the water extract of Gekko Swinhonis Radix Actinidiae Chinensis;
Adding the volume of entry and the part by weight of Gekko Swinhonis Radix Actinidiae Chinensis mixture during lixiviate is 10～15ml: 1g;
Preferred 3～6 hours of reflux extracting time;
Preferred 1～3 time of reflux, extract, number of times.
With Gekko Swinhonis Radix Actinidiae Chinensis water extract provided by the invention is that effective ingredient suppresses the tumor cell experiment, the result shows, Gekko Swinhonis Radix Actinidiae Chinensis water extract has the inhibition effect to hepatoma cell strain Bel-7402, suppression ratio can reach 60% under low concentration, shows that it can be used to prepare the medicine for the treatment of hepatocarcinoma.The present invention has widened the medical applications field of this compound recipe of Gekko Swinhonis Radix Actinidiae Chinensis.
The specific embodiment
The water extract I of embodiment 1, preparation Gekko Swinhonis Radix Actinidiae Chinensis
Commercially available house liyard powder and rice according to 4: 1 mix homogeneously of mass ratio, are put into frying pan then and stir-fried,, remove rice, take out the house liyard powder cold of drying in the air, obtain rice and fry a house liyard powder until the rice buff.
Weighing 315 gram Radix Actinidiae Chinensis and 45 restrain house liyard powders, mix homogeneously respectively.Add 4500ml water, refluxed 4 hours, extract; Backflow lixiviate 3 times merges lixiviating solution, obtains the water extract of Gekko Swinhonis Radix Actinidiae Chinensis.This Gekko Swinhonis Radix Actinidiae Chinensis water extract at 38 ℃ of rotary evaporations, is removed and to desolvate, and until obtaining brown paste, vacuum drying 24h obtains the water extract I of Gekko Swinhonis Radix Actinidiae Chinensis.
The water extract II of embodiment 2, preparation Gekko Swinhonis Radix Actinidiae Chinensis
Commercially available house liyard powder and rice according to 3: 1 mix homogeneously of mass ratio, are put into frying pan then and stir-fried,, remove rice, take out the house liyard powder cold of drying in the air, obtain rice and fry a house liyard powder until the rice buff.
Weighing 135 gram Radix Actinidiae Chinensis and 45 restrain house liyard powders, mix homogeneously respectively.Add 1800ml water, refluxed 3 hours, extract; Backflow lixiviate 2 times merges lixiviating solution, obtains the water extract of Gekko Swinhonis Radix Actinidiae Chinensis.This Gekko Swinhonis Radix Actinidiae Chinensis water extract at 30 ℃ of rotary evaporations, is removed and to desolvate, and until obtaining brown paste, vacuum drying 24h obtains the water extract II of Gekko Swinhonis Radix Actinidiae Chinensis.
The water extract III of embodiment 3, preparation Gekko Swinhonis Radix Actinidiae Chinensis
Commercially available house liyard powder and rice according to 6: 1 mix homogeneously of mass ratio, are put into frying pan then and stir-fried,, remove rice, take out the house liyard powder cold of drying in the air, obtain rice and fry a house liyard powder until the rice buff.
Weighing 405 gram Radix Actinidiae Chinensis and 45 restrain house liyard powders, mix homogeneously respectively.Add 6750ml water, refluxed 6 hours, extract, obtain the water extract of Gekko Swinhonis Radix Actinidiae Chinensis.This Gekko Swinhonis Radix Actinidiae Chinensis water extract at 45 ℃ of rotary evaporations, is removed and to desolvate, and until obtaining brown paste, vacuum drying 24h obtains the water extract III of Gekko Swinhonis Radix Actinidiae Chinensis.
Embodiment 4, Gekko Swinhonis Radix Actinidiae Chinensis cell experiment
Cell strain: hepatoma cell strain Bel-7402 is provided by the Shanghai cell
Screening technique: sulphonyl rhodamine B (Sulforhodamine B, SRB) protein staining method;
Action time: 72 hours
Experimental unit: The National Center for Drug Screening
The Gekko Swinhonis Radix Actinidiae Chinensis water extract I that embodiment 1 is obtained suppresses the cell experiment of tumor.The method of experiment is: the hepatoma cell strain Bel-7402 of the trophophase of taking the logarithm is inoculated in 96 well culture plates, and cell is placed 37 ℃, 5%CO
2Pre-after adherent 24 hours in the incubator, abandoning supernatant, the Gekko Swinhonis Radix Actinidiae Chinensis water extract of adding variable concentrations.Each concentration is provided with three multiple holes.And the normal saline solvent that is provided with respective concentration contrasts and acellular withered hole.Tumor cell is at 37 ℃, 5%CO
2Cultivated 72 hours under the condition, the culture fluid that inclines adds SRB solution and trihydroxy methyl ammonia and methane (Tris) solution successively, measures each hole absorbance under microplate reader under the 515nm wavelength.And calculate by following formula, the results are shown in table 1.
Table 1, Gekko Swinhonis Radix Actinidiae Chinensis water extract are tested the inhibition of hepatoma cell strain Bel-7402
The above results shows that Gekko Swinhonis Radix Actinidiae Chinensis water extract provided by the invention is better to the inhibition effect of hepatoma cell strain Bel-7402 than independent Radix Actinidiae Chinensis and house liyard powder water extract, especially has high inhibitory under the low concentration of 0.063mg/ml.
This shows that the performance of compound recipe drug effect is not the simple addition of single active ingredient, but exist between the composition in the compound recipe collaborative or produce new complexing actions such as pharmacology.For this compound recipe, may be because the synergism that produces between house liyard powder and the Radix Actinidiae Chinensis makes it under very low concentration, be better than single medicinal material for the inhibition effect of hepatocarcinoma.This result shows that also this compound recipe can be used to prepare the medicine for the treatment of hepatocarcinoma simultaneously.The present invention has widened the medical applications field of Gekko Swinhonis Radix Actinidiae Chinensis.
Claims (5)
1, a kind of water extract that is used for the treatment of the Gekko Swinhonis Radix Actinidiae Chinensis of hepatocarcinoma is rice to be fried back Gekko Swinhonis dry powder body and Radix Actinidiae Chinensis carry out water extraction together and obtain;
Described rice is fried being prepared as follows of house liyard powder:
With house liyard powder and rice according to weight ratio 3～6: 1 mix homogeneously, put into marmite then and stir-fry, until the rice buff, remove rice, take out the house liyard powder cold of drying in the air;
The preparation of described house liyard powder and Radix Actinidiae Chinensis water extract:
With above-mentioned after rice is fried house liyard powder and after Radix Actinidiae Chinensis mixes by weight 1: 3～9, add distilled water, reflux is carried out lixiviate, obtains the aqueous extract of Gekko Swinhonis Radix Actinidiae Chinensis; Then at 30～45 ℃ of rotary evaporations, remove and desolvate, until obtaining brown paste, vacuum drying 24h obtains the water extract of Gekko Swinhonis Radix Actinidiae Chinensis.
2, the water extract that is used for the treatment of the Gekko Swinhonis Radix Actinidiae Chinensis of hepatocarcinoma as claimed in claim 1 is characterized in that: the part by weight of the volume of described adding distilled water and Gekko Swinhonis Radix Actinidiae Chinensis mixture is 10～15ml: 1g.
3, the water extract that is used for the treatment of the Gekko Swinhonis Radix Actinidiae Chinensis of hepatocarcinoma as claimed in claim 1, it is characterized in that: described backflow extraction time is 3～6 hours.
4, the water extract that is used for the treatment of the Gekko Swinhonis Radix Actinidiae Chinensis of hepatocarcinoma as claimed in claim 1 is characterized in that: described backflow lixiviate number of times is 1～3 time.
5, the described purposes of water extract in the medicine of preparation treatment hepatocarcinoma that is used for the treatment of the Gekko Swinhonis Radix Actinidiae Chinensis of hepatocarcinoma of one of claim 1 to 4.
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| CNB2006100669938A CN100546591C (en) | 2006-04-04 | 2006-04-04 | A kind of Gekko Swinhonis Radix Actinidiae Chinensis water extract that is used for the treatment of hepatocarcinoma |
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| CNB2006100669938A CN100546591C (en) | 2006-04-04 | 2006-04-04 | A kind of Gekko Swinhonis Radix Actinidiae Chinensis water extract that is used for the treatment of hepatocarcinoma |
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| CN101049326A CN101049326A (en) | 2007-10-10 |
| CN100546591C true CN100546591C (en) | 2009-10-07 |
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| CN106620835A (en) * | 2017-02-23 | 2017-05-10 | 福建吉特瑞生物科技有限公司 | Medical collagen film with wound healing function |
| CN109394804A (en) * | 2018-12-10 | 2019-03-01 | 曾付英 | A kind of Chinese medicine composition for the treatment of cancer disease |
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Non-Patent Citations (2)
| Title |
|---|
| 壁虎藤梨根治疗胃癌临床及实验研究. 朱秀山，许继平，黄德辉，黄国荣，应栩华.中国民间疗法，第3期. 1999 |
| 壁虎藤梨根治疗胃癌临床及实验研究. 朱秀山，许继平，黄德辉，黄国荣，应栩华.中国民间疗法，第3期. 1999 * |
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Granted publication date: 20091007 Termination date: 20130404 |