CN100535634C - Biochip detection method with light intensity real-time detection and detection system thereof - Google Patents
Biochip detection method with light intensity real-time detection and detection system thereof Download PDFInfo
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- CN100535634C CN100535634C CNB2004100090447A CN200410009044A CN100535634C CN 100535634 C CN100535634 C CN 100535634C CN B2004100090447 A CNB2004100090447 A CN B2004100090447A CN 200410009044 A CN200410009044 A CN 200410009044A CN 100535634 C CN100535634 C CN 100535634C
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- 238000001514 detection method Methods 0.000 title claims abstract description 39
- 238000000018 DNA microarray Methods 0.000 title claims abstract description 12
- 238000011897 real-time detection Methods 0.000 title claims description 6
- 229910052724 xenon Inorganic materials 0.000 claims abstract description 16
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 230000003287 optical effect Effects 0.000 claims description 32
- 238000012360 testing method Methods 0.000 claims description 9
- 238000010998 test method Methods 0.000 claims description 6
- 230000023077 detection of light stimulus Effects 0.000 claims description 4
- 230000005284 excitation Effects 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims description 2
- 239000011248 coating agent Substances 0.000 claims description 2
- 238000000576 coating method Methods 0.000 claims description 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 7
- 239000000523 sample Substances 0.000 abstract description 2
- 239000012472 biological sample Substances 0.000 abstract 1
- 230000002596 correlated effect Effects 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A biochip detecting method with real-time light intensity detection and its detecting system features that a light density disk is added to the light path of exciting light to regulate light intensity, a light detecting system is installed under the slide holder to detect the light intensity of the light emitted from xenon lamp to the sample to be detected, which truly reflects the true condition of exciting light source, and the light intensity is recorded in computer. The invention can adjust the light intensity through the light density disc, and can also adjust the exposure of the biological sample wafer, thus avoiding the detection error caused by the light intensity difference or uniformity change of the exciting light source in the biochip detection, and ensuring the true and reliable detection data of the biochip detection system.
Description
Affiliated technical field
The present invention relates to a kind of biochip test method and detection system thereof that light intensity detects in real time that have.
Technical background
In the biological chips detection system testing process; because the serviceable life of exciting light sources xenon lamp and the factors such as instability of line voltage; usually can cause that the light intensity of xenon source and homogeneity change; so not only can make biological chips detection system in testing process because the variation of light source intensity and detection data that the homogeneity difference causes biological chips detection system produce difference; cause misjudgment; and photobleaching can appear, biochip is caused damage.At present, multiple biochip supplies analysis is arranged both at home and abroad, but in these instruments, all do not adopt real-time detection technique of light intensity and adjustment measure, change the influence that is brought to solve light source intensity.
Summary of the invention
Technology of the present invention is dealt with problems and is: for overcome that biological chips detection system occurs in testing process by the caused detection error problem of light source intensity difference, a kind of biological chips detection system that light intensity detects in real time that has is provided, make and in time to be detected when the light source intensity change conditions appears in biological chips detection system in testing process, and in time take measures by computing machine, adjust the exposure of optical density dish or control print, make that the detection data are true and reliable.
The technical solution adopted for the present invention to solve the technical problems is: have the biochip test method that light intensity detects in real time, it is characterized in that: in the exciting light light path of biological chips detection system, add an optical density dish, to adjust light intensity, an optical detection system is installed below slide holder, its objective is the light intensity of the light on the detected print of arrival that detection sent by xenon source, this light intensity value has reflected the truth of excitation source light intensity really, this light intensity value record to some extent in computing machine, when this light intensity value change, computer system is judged according to the signal that testing circuit provides, in time take measures, adjust the exposure of optical density dish or adjustment print, to guarantee to detect the true and reliable of data.
The biological chips detection system of realizing said method and designing with the real-time detection of light intensity, comprise optical system, the emission interference filter, computing machine, slide holder, xenon source, excite interference filter, it is characterized in that: after xenon source, add the optical density dish that is useful on the adjusting light intensity, photo-detector is installed below slide holder, the light that xenon lamp sends is through the optical density dish, after exciting interference filter, shine on the slide holder again, light intensity detector promptly may detect this light signal, after the integrated operational amplifier amplification, send into computing machine and handle, adjust the exposure of optical density dish or adjustment print again by computer control.
Principle of the present invention is: biological chips detection system can carry out online detection to the light intensity and the homogeneity of xenon lamp exciting light sources by inner light intensity detection system, and by computing machine according to the light intensity value that the is detected control of being correlated with, both can adjust light intensity by the optical density dish, also can adjust the exposure of biological print, to reduce biological chips detection system caused detection error of difference owing to exciting light sources in detection.
The invention has the beneficial effects as follows, has the biological chips detection system that light intensity is surveyed, can detect in time, effectively whether xenon source working properly, whether light intensity even, and can control adjustment by computing machine easily, the required electric current of light intensity probe portion circuit is very little, primary power is not impacted, and circuit only adopts photo-detector and integrated transporting discharging device, resistance, electric capacity etc., and the optical density dish is simple in structure, it is easy to adjust, economical, safe to install, reliably.
Description of drawings
Fig. 1 is a systematic schematic diagram of the present invention.
Embodiment
As shown in Figure 1, the embodiment of the invention is by image acquistion system 1, optical system 2, emission interference filter 3, computing machine 4, slide holder 5, photo-detector 6, integrated transporting discharging 7, xenon source 8, optical density dish 9 and excite interference filter 10 to form.Open xenon lamp power supply, the light that xenon lamp 8 sends is through optical density dish 9, after exciting interference filter 10, shine on the slide holder 5 again, at this moment, the light intensity detector 6 that is installed under the slide holder 5 promptly may detect this light signal, and this signal is after operational amplifier 7 amplifies, flow to computing machine 4, the light intensity data that 4 bases of computing machine receive is in time handled, and adjusts the exposure of optical density dish 9 or adjustment print, to eliminate the error information detection that causes because of the light source intensity variation.During detection, be placed on print on the slide holder 5 after excitation, the target molecule that is marked with fluorescent dye on the print is gone into optics system 2 at the monochromatic fluorescence that produces down of exciting through emission spike interference filter 3 is laggard, optical system 2 is delivered to data acquisition system (DAS) 1 with the picture intelligence of catching, the picture intelligence that data acquisition system (DAS) 1 is caught optical system 2 changes digital signal into delivers to computing machine 4, again the detection data processing of being correlated with by computing machine 4.
Claims (5)
1, has the biochip test method that light intensity detects in real time, it is characterized in that: in the exciting light light path of biological chips detection system, add an optical density dish, to adjust light intensity, an optical detection system is installed below slide holder, light intensity on the detected print of arrival that detection is sent by xenon source, this light intensity value has truly reflected the truth of excitation source light intensity, this light intensity value record to some extent in computing machine, when this light intensity value change, computer system is judged according to the signal that testing circuit provides, in time take measures, adjust the exposure of optical density dish or adjustment print, to guarantee to detect the true and reliable of data.
2, the biochip test method with the real-time detection of light intensity according to claim 1, it is characterized in that: the exposure of described adjustment optical density dish or adjustment print is meant: when the light intensity of exciting light sources changes, computing machine can spiral according to the light intensity signal automatic control motor drive optical density that detects and forward the desired position to adjust the incident intensity of exciting light, and computing machine also can change the exposure that being provided with of time shutter adjusted print as required.
3, the biochip test method with the real-time detection of light intensity according to claim 1, it is characterized in that: described optical density dish is to be made of the optical device through coating film treatment, and the transmitance of its light is evenly to distribute by 99%～5% in 0 °～360 ° scopes.
4, has the biological chips detection system that light intensity detects in real time, it comprises optical system, the emission interference filter, computing machine, slide holder, xenon source, excite interference filter, it is characterized in that: after xenon source, add the optical density dish that is useful on the adjusting light intensity, photo-detector is installed below slide holder, the light that xenon lamp sends is through the optical density dish, after exciting interference filter, shine on the slide holder again, light intensity detector promptly may detect this light signal, after the integrated operational amplifier amplification, send into computing machine and handle, adjust the exposure of optical density dish or adjustment print again by computer control.
5, the biological chips detection system with the real-time detection of light intensity according to claim 4, it is characterized in that: described photo-detector adopts the GT101 photodetector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100090447A CN100535634C (en) | 2004-04-26 | 2004-04-26 | Biochip detection method with light intensity real-time detection and detection system thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100090447A CN100535634C (en) | 2004-04-26 | 2004-04-26 | Biochip detection method with light intensity real-time detection and detection system thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1690690A CN1690690A (en) | 2005-11-02 |
| CN100535634C true CN100535634C (en) | 2009-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2004100090447A Expired - Fee Related CN100535634C (en) | 2004-04-26 | 2004-04-26 | Biochip detection method with light intensity real-time detection and detection system thereof |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI464388B (en) * | 2012-08-17 | 2014-12-11 | Wistron Corp | Light calibration device, biological detection calibration system and operating method thereof |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7767441B2 (en) * | 2007-10-25 | 2010-08-03 | Industrial Technology Research Institute | Bioassay system including optical detection apparatuses, and method for detecting biomolecules |
| US7811810B2 (en) | 2007-10-25 | 2010-10-12 | Industrial Technology Research Institute | Bioassay system including optical detection apparatuses, and method for detecting biomolecules |
| CN105758834B (en) * | 2016-04-26 | 2018-11-27 | 福州大学 | A kind of biochip test method of induced with laser and CCD acquisition |
| CN111487037A (en) * | 2020-04-16 | 2020-08-04 | 中国科学院微电子研究所 | System and method for detecting uniformity of light source |
| CN113008798A (en) * | 2021-03-15 | 2021-06-22 | 上海华力微电子有限公司 | Illumination light path, defect detection device and light intensity measurement method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87207366U (en) * | 1987-07-31 | 1988-09-07 | 福建省光学技术研究所 | Apparatus for light intensity stabilization of laser |
| WO2003038413A1 (en) * | 2001-11-01 | 2003-05-08 | Diachip Co., Ltd. | Apparatus for analyzing florescent image of biochip |
| CN2581981Y (en) * | 2002-10-29 | 2003-10-22 | 南京大渊生物技术工程有限责任公司 | Recognizer for biochip |
-
2004
- 2004-04-26 CN CNB2004100090447A patent/CN100535634C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87207366U (en) * | 1987-07-31 | 1988-09-07 | 福建省光学技术研究所 | Apparatus for light intensity stabilization of laser |
| WO2003038413A1 (en) * | 2001-11-01 | 2003-05-08 | Diachip Co., Ltd. | Apparatus for analyzing florescent image of biochip |
| CN2581981Y (en) * | 2002-10-29 | 2003-10-22 | 南京大渊生物技术工程有限责任公司 | Recognizer for biochip |
Non-Patent Citations (3)
| Title |
|---|
| CCD生物芯片检测仪设计. 唐小萍等.微纳电子技术，第5期. 2002 * |
| 一种自适应视频信号调光的新方法. 廖开轩.光电工程，第21卷第5期. 1994 * |
| 自发光蛋白质芯片检测仪的设计. 陈兴俊等.微纳电子技术，第1期. 2004 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI464388B (en) * | 2012-08-17 | 2014-12-11 | Wistron Corp | Light calibration device, biological detection calibration system and operating method thereof |
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| Publication number | Publication date |
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| CN1690690A (en) | 2005-11-02 |
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