CN100529073C - Clone and application for pig growth character gene INPP5F - Google Patents
Clone and application for pig growth character gene INPP5F Download PDFInfo
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- CN100529073C CN100529073C CNB2007100517728A CN200710051772A CN100529073C CN 100529073 C CN100529073 C CN 100529073C CN B2007100517728 A CNB2007100517728 A CN B2007100517728A CN 200710051772 A CN200710051772 A CN 200710051772A CN 100529073 C CN100529073 C CN 100529073C
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Abstract
The present invention belongs to animal gene engineering field, more specificly pig growth character gene INPP5F clone and its uses. In the present invention pig growth character gene INPP5F is cloned, DNA sequence is indicated as the following list SEQ ID NO:1. The 279 bp point of SEQ ID NO:1 has a A279-G279 base mutation which result in Hinc II-RFLP polymorphism. The present invention also discloses pig growth character gene INPP5F preparation method, primer design and its uses in assistant selection for pig molecular mark.
Description
Technical field
The invention belongs to animal gene engineering technology field, the clone who is specifically related to pig growth traits genes involved INPP5F gene with and application on marker assisted selection.
Background technology
China raises pigs with a long history, existing 6000～10000 years.Pig industry is the important component part of livestock industry always, and in recent years, pork accounts for about 65% of meat gross output always, for the life that improves the people, increases farmers' income, and guarantees that social stability plays an important role.Especially along with the reform of China's economic system, peasant's merchandise consciousness progressively strengthens, pig industry just by traditional-family's sideline production to specialization, mass-producing, intensification,
The batch production direction develops rapidly.Therefore, the development of quickening genetic thremmatology is used to support that pig industry has very important effect.
The major objective proterties of selecting in the pig breeding comprises following two aspects, be carcass trait on the one hand, comprise the metric (weighing or the like) of each component of trunk and the weight percent (as dressing percentage, leg stern ratio, leg stern meat bone rate, leaf fat rate and lactones or the like) of each composition as back-fat thickness, eye muscle area, trunk length, small intestine length, various internal organ; Be the speed of growth on the other hand, its testing index generally comprises 60 age in days body weight, average daily gain in early stage, later stage average daily gain and full phase average daily gain or the like.
Along with the increase of people to the meat requirement, livestock industry must be shortened the cattle breeding phase, more provides meat product for people.Growth traits belongs to quantitative character, by controlled by multiple genes and there is key-gene (major gene) effect.The development of Protocols in Molecular Biology, make people can seek the key-gene or the molecule marker closely linked of control growing proterties at dna level with it, in breeding process, be used for marker assisted selection (markerassisted selection, MAS), select progress so that improve, improve the pig growth traits better, satisfy people's needs, obtain bigger economic benefit simultaneously.
By the candidate gene method, found some to influence the gene of the speed of growth of pig.Be positioned at growth hormone gene (the Growth Hormon on No. 12 karyomit(e)s of pig, GH) be the core gene of regulating and control animal growth in the neurosecretion in the growth axis, its product tethelin has the effects such as metabolic, that promotion is grown of regulating, be the main candidate relevant with the growth of pig, many scholars have carried out extensive studies to its genotype and the relation of the production traits.Experimental results show that: exogenous pig GH can promote the metabolism of pig in vegetative period, strengthen digestive tube digesting and assimilating to feed, impel mytolin, collagen protein synthesis to increase and the fatty deposits minimizing, insulin-like growth factor I GF-I concentration in the blood is risen to some extent, and the long and has improved the average daily gain of pig, and (he is flooded ability etc. to have increased lean ratio, Porcine somatotropin and pig industry, Jiangxi animal and veterinary magazine, 1994,3:12-14).(square the U.S. and Britain etc. such as Fang Meiying, six kind pig growth hormone gene seat genetic polymorphisms detect, zoological research, 1998,19 (4): 334-336) (Song Chengyi etc. such as Song Chengyi, pig GH Gene Partial mutational site is to the influence of growth performance, heredity, 2001,23 (5): 427-430) elementary (Yu Pei is elementary for Yu Pei, fragrant pig growth hormone gene different genotype is to the influence of growth performance, Shanghai Communications University's journal, 2006,24 (4), 326-329) also once found different GH genotype and the average daily gain of pig Different Month, the Different Month body weight has significant correlation.(Guan Xuemin etc. such as Guan Xuemin, the sudden change of pig muscle amicine gene 5 '-control region and the correlation analysis of production performance, northwest agricultural journal, 2006,15 (2): 7-9) discover that in landrace, Large White, duroc, Shanxi bacon hogs new lines (SD-III system) and 5 kinds of Shanxi local variety length pig myostatin (myostatin, MSTN) ring significantly the birth ghost image by genotype.
(myostatin is to influence the supressor that Skeletal Muscle Growth is grown MSTN) to be positioned at No. 15 chromosomal myostatins.Its to lean pork amount and fatty deposits have significant effects (Sonstegard T S etc., Myostatin maps to porcine chromosome 15 by linkageand physical analyses.Anim Genet, 1998,29:19-22).Its sudden change of researchs such as Grobet L report can cause muscle growth (Grobet L etc., A deletion in the bovine myostatin gene causes the double-muscled phenotype incattle.Nat Genet, 1997,17:71-74).(Te Pas M F etc. such as Te Pas, Influences of myogenin genotypes onbirth weight, growth rate, carcass weight, backfat thickness, and lean weight of pigs.J AnimSci, 1999,77:2352-2356) expression level of finding Myogenin mRNA simultaneously can show the satellite cell number that is activated in the muscle, the speed of growth of reflection muscle.By pig MSTN gene changes of expression level in growth course is also illustrated relevant (the ji S etc. with the speed of growth of MSTN expression of gene, Myostatin expression in porcine tissues:tissue specificity and developmental andpostnatal egulation.Am.J.Physiol, 1998,275:1265-1273).
Pig MC4R gene is considered to one of major gene that influences the grow-finish proterties (Kim K S etc., Linkage and physical mappingof the porcine melanocortin-4receptor (MC4R) gene.Journal of Animal Science, 2000,78:791-792).It is positioned at karyomit(e) No. 1.(Xiao Shijun etc. such as Xiao Shijun, pig resource family MC4R genescan and with the correlation analysis of fatty character, journal of animal science and veterinary medicine, 2006,37 (9): 841-845) adopt the PCR-TaqI-RFLP technology, 9 Chinese native pig breeds have been detected, 3 external pig kinds and 1 612 individualities and white Du Luoke * 418 F individuality of painted face in Beijing opera resource family of cultivating the pig kind are imitated the heritable variation in site MC4R gene D298N master, growth traits significant correlations such as MC4R genotype and day weight gain are found in statistical study, simultaneously, 240 ages in days of GG genotype individuality are heavy, to 240 age in days average daily gains obviously greater than AA genotype individuality.(Kim K S etc., Linkage and physical mapping of theporcine melanocortin-4 receptor (MC4R) gene.Journal of Animal Science, 2000,78:791-792 such as Kim; Kim KS etc., A missense vari ant of the porcine melanocortin-4 receptor (MC4R) gene is associated withfatness, growth, and feed intake traits.Mammalian Genome, 2000,1:131-135) studies show that: pig MC4R is strong correlation in the genotype in this mutational site and the thickness of backfat of some strains and the food consumption of growth rate and all strains.
In addition, pork insulin like growth factor (IGF-1), the PIT1 gene, ob gene (LEP) etc. all is considered to relevant with the speed of growth of pig.
INPP5F (inositol polyphosphate-5-phosphatase F) gene is the gene of a coding inositol monophosphate enzyme, and the inositol monophosphate enzyme participates in the engulfing and efflux effect of cell of nerve ending.Jonathan etc. utilize tissue-specific chip technology, and one of them the transcript Inpp5f_v2 that verifies this gene is the maternal marking on mouse, this transcript special expression and marking in the brain of mouse.(Jonathan etc., A NovelVariant of Inpp5f Is Imprinted in Brain, and Its Expression Is Correlated with DifferentialMethylation of an Internal CpG Island.MOLECULAR AND CELLULAR BIOLOGY, 2005,13:5514-5522).Afterwards discover the brain of Inpp5f_v2 people's fetus, heart and tongue the inside also are maternal imprinted gene.The genome research result of people and mouse is positioned the INPP5F gene for people's 10 (10q26.11) number karyomit(e) and No. 7 karyomit(e)s of mouse respectively.The INPP5F gene has the transcript of three alternative splicings on the people, and its dna sequence dna total length is 103051bp, and has had to a transcript on mouse at present, and its DNA total length is 85072bp.By the sequence similarity between people and the mouse is found relatively it is high conservatives that Inpp5f_v2 has 5 exons between people and mouse.The inositol monophosphate enzyme participates in the cytophagy of nerve ending and effluxes effect, and this process is closely-related with the recirculation of synaptic vesicle with coming off of clathrin coating, hint that this gene might be at (the Arai etc. that play an important role aspect the growth of body brain and the neonatal survival rate, Developmental changes of synaptojanin expression in the human cerebrum and cerebellum.Dev.Brain Res..2001,129:1-9).But up to the present, still do not see the report of comprehensive research INPP5F gene function.The polymorphism of research mutational site in colony, and carry out the very strong means that the proterties association analysis is the research gene function.So the applicant has carried out polymorphic research and association analysis to the exon of the part of this gene, in the hope of finding its function.
Summary of the invention
The objective of the invention is to the clone pig growth character gene INPP 5 F, seek the mutational site of INPP5F gene and as the detection method of pig growth traits gene pleiomorphism, for marker-assisted breeding provides a kind of useful molecule marker.
The present invention is achieved in that
One boar growth related gene INPP5F, its dna sequence dna is as described in the sequence table SEQ ID NO:1.
The INPP5F genome sequence total length of pcr amplification is 355bp, is as accompanying drawing 2 described extron 20 sequences entirely wherein.
In the INPP5F Gene Partial dna sequence dna that is obtained 1 base mutation as the described A279-G279 of sequence table SEQ ID NO:1 is arranged, cause Hinc II-RFLP (Restriction Fragment Length Polymorphism) polymorphism.
A kind of method for preparing pig growth traits INPP5F gene DNA sequence, according to following steps:
Personnel selection INPP5F gene cDNA is the information probe, does the homologous sequence screening, obtains the expressed sequence tag (EST) of similarity more than 90%; Splice pig EST-contig then; According to EST-contig design primer, primer is used for pcr amplification, to PCR product purification, clone and the order-checking that obtains, carries out sequential analysis, obtains as the described dna sequence dna of sequence table SEQ ID NO:1.
The applicant uses above-mentioned PCR-RFLP method the base mutation of the 279th of pig INPP5F gene is detected.
Below the invention will be further described:
1, the clone of the 20th exon of INPP5P gene
(1) design of primers:
(the GenBank number of including: NM_014937) be the information probe of personnel selection INPP5F gene cDNA, utilize the BLAST instrument among the NCBI in GenBank pig est database, to do the homologous sequence screening, obtain a series of similaritys and be the ESTs (fragment length is greater than 100bp) more than 90%, the number of including of these ESTs is inquired about corresponding sequence with ENTREZ (http://www.ncbi.nlm.nih.gov/Web/Search/index.html) in NCBI, use the ASSEMBLY program construction pig EST-contig among the GeneTool then.According to EST splicing sequences Design primer, sequence is as follows:
5 ' GACTCCTACCACTCCGATGAAT 3 ' (forward),
5 ' GCTGGGTCACTTTGTTTTGTG 3 ' (oppositely).
(2) purifying of PCR product, clone and order-checking:
The purifying of PCR product: under ultraviolet lamp, contain the segmental gel of purpose from the cutting-out of low melting-point agarose gel, put into 1.5mL Ependorff pipe, use PCR product purification test kit (available from Promega company) purified pcr product then, according to test kit specification sheets operation, concrete steps are the S of 300 μ L in the gel of every 100mg
1Liquid melts in 65 ℃ of incubation 10min to gel, fully with S
1/ DNA mixture changes over to receiving post, and the centrifugal 30s of 9200g makes slurries extrude by Minicolumn.Waste liquid in the following pipe is outwelled, added the W of 500 μ L again
1Liquid is to pipe, and the centrifugal 15s of 9200g outwells the waste liquid in the pipe.The W that adds 500 μ L again
1Liquid leaves standstill 1min, and the centrifugal 30s of 9200g takes off Minicolumn and packs in the 1.5ml Ependorff pipe, adds aqua sterilisa or the TE liquid of 25 μ L, leaves standstill after the 1min, and the centrifugal 1min of 9200g is stored in the Ependorff pipe with eluted dna.
Ligation: purified pcr product is connected with pGEM-T carrier (available from Promega company), the ligation cumulative volume is 5 μ l, comprising 2.5 μ l, 2 * buffer, 0.5 the T carrier of μ l, 0.5 the purified pcr product of μ l, 0.5 the T4 ligase enzyme of μ l adds 1 μ l aqua sterilisa at last and puts 4 ℃ of water-baths and spend the night.
The preparation of competent cell: the single colony inoculation of DH5 α of picking is in 2ml LB from 37 ℃ of fresh flat boards of having cultivated 16-20h, in 37 ℃ of shaking culture 3h, switching 1ml bacterium liquid is in the saline bottle that contains 30ml LB, continuation is at 37 ℃ of about 4h of shaking culture, treat that when OD600 reaches 0.3-0.4 saline bottle being put ice bath from the shaking table taking-up cools off 10-15min, then bacterium liquid is changed in the centrifuge tube in 4 ℃ 4, the centrifugal 10min of 000g is with collecting cell, centrifuge tube is inverted to abandon clean nutrient solution, ice the resuspended precipitation of CaCl2 of the 0.1mol/L of precooling with 10ml, ice bath 30min, repeat 4 ℃ 4, the centrifugal 10min of 000g once, with the resuspended precipitation of CaCl2 of the 0.1mol/L of 4ml ice precooling, it is standby to put 4 ℃ of preservations.
Transform: get 100-120 μ l competent cell under the sterile state in 1.5ml Ependorff pipe, the connection product of 5 μ l is added mixing, place 30min on ice, 42 ℃ of heat shock 90s, do not shake the Ependorff pipe therebetween, take out back ice bath 3 ~ 4min, add the LB liquid nutrient medium of 400 μ l antibiotic-frees, 37 ℃ of shaking culture 45min.Get 100 μ l and coat in advance that 4h has been coated with on the agar plate of IPTG (Isopropylthio-β-D-galactoside, isopropylthio-) and X-gal, be inverted cultivation after keeping flat 1h for 37 ℃.
(4) the dna sequence dna homology search is identified:
By the American National biotechnology (NCBI of information center, National Center for Biotechnology Information, http://www.ncbi.nlm.nih.gov) BLAST of website (Basic Local Alignment Search Tool) software, the known physiological function gene of announcing in the dna sequence dna that order-checking back is obtained and the GenBank database carries out sequence homology relatively, with evaluation with obtain the function information of this dna sequence dna.
2.PCR-RFLP diagnostic method is set up
(1) primer sequence
INPP5F：PL?15′-GACTCCTACCACTCCGATGAAT-3′
PR2?5′-GCTGGGTCACTTTGTTTTGTG-3′
This primer amplification fragment length 355bp.
(2) pcr amplification condition
PCR reaction cumulative volume 20 μ l, wherein the about 100ng of pig genomic dna contains 1 * buffer (available from Promega company), 1.5mmol/LMgCl
2, the dNTP final concentration is 150 μ mol/L, the primer final concentration is 0.2 μ mol/L, 2U Taq archaeal dna polymerase (available from Promega company).The pcr amplification program is: 94 ℃ of 4min, and the 94 ℃ of 30s that circulate 34 times, 55 ℃ of 30s, 72 ℃ of 20s, last 72 ℃ are extended 5min.The PCR reaction product detects with 2% agarose gel electrophoresis and takes pictures.
(3) RFLP testing conditions
PCR product endonuclease reaction volume is 10 μ l, 1 * buffer, 1 μ l wherein, and PCR product 3-5 μ l, restriction enzyme HincII is 0.2 μ l (2U), uses H
2O supplies 10 μ l, and with centrifugal behind the sample mixing, 37 ℃ of water-bath 4h detect enzyme with 2% agarose gel electrophoresis and cut the result, and the record genotype is taken pictures under ultraviolet lamp.
3. mark property association analysis
Utilizing the applicant's molecular biology genetic breeding laboratory Large White colony to do the proterties association analysis, 236 DNA samples (taking from the pig ear tissue) are used for genotype detection.The proterties of being analyzed has part growth traits and part reproductive trait.The GLM program is by the association analysis of carrying out with drag between genotype and proterties in the utilization SAS Vergion8.1 software (a kind of common computer software of public use), and analyzing has two steps, sets up earlier and rejects all systemic effects as drag:
yi＝μ+Gi+εi，
Wherein, yi is the proterties phenotypic number, and μ is a population mean, and Gi is genotype effect (comprising the Ai additive effect, the Di dominant effect).ε
iBe random error, suppose obey N (0, I σ
2) distribute.
Effect of the present invention is:
1, the clone of pig INPP5F Gene Partial dna sequence dna
Pcr amplification product is special PCR product through the demonstration of 2% agarose gel electrophoresis detected result.The PCR product is reclaimed the order-checking of purifying rear clone, and sequencing result shows that the length of PCR product is 355bp.It all is extron 20 sequence (shown in Fig. 2, SEQ ID NO:1).Sequencing result shows and has two allelotrope of A, G at this segmental 279bp place that order-checking peak figure sees Fig. 4.
2, the PCR-RFLP diagnostic method is set up
Obtain 355bp specific amplified fragment (seeing Fig. 3 for details) with INPP5F-PL1 and INPP5F-PR1 amplification pig genomic dna.There is HincII restriction enzyme site (GTY*RAC) in found that of order-checking in this 355bp fragment, wherein the 281bp place is the polymorphism point of contact, be arranged in extron 20, INPP5F gene acquisition length with INPP5F-PL1 and INPP5F-PR1 amplification pig is the PCR product of 355bp, enzyme is cut and is produced three kinds of genotype, and genotype BB type has 281bp and 74bp two bands, and the AA type has only the band of 355bp, heterozygote AB type has 355bp, 281bp and 74bp three bands.(wherein the band of 74bp be can't see in the drawings)
3, mark property association analysis
Pig INPP5F gene the 20th exon HincII-RFLP pleomorphism site and part growth, reproductive trait are carried out association analysis, genotype detection result shows that the AA genotype has 113 in 236 individualities, the AB genotype has 98, the BB genotype has 25 (because indivedual data disappearances of measuring proterties, therefore the actual value that obtains is shown in table 2, table 3), resultant relevant proterties is 30-100Kg day weight gain and omnidistance day weight gain.
As shown in Table 2, A allelotrope is the favourable mark of pig 30-100Kg day weight gain and omnidistance day weight gain, and AA genotype mark can be used for improving the selective marker (molecule marker) of growth speed of pigs.
Table 2INPP5F gene pleiomorphism is to the influence of 30-100Kg day weight gain
Table 3INPP5F gene pleiomorphism is to the influence of omnidistance day weight gain
Description of drawings
Sequence table SEQ ID NO:1 is the pig growth traits genes involved INPP5F dna sequence dna that the present invention clones.
Fig. 1: be the schema of the present invention about the preparation of INPP5F gene.
Fig. 2: being pig INPP5F gene 20 exon sequences among the present invention, is allelic mutational site in the bracket at the 279th bit base place wherein.
Fig. 3: be the electrophoretogram of the extension increasing sequence of pig INPP5F gene the 20 exon among the present invention, clip size is 355bp (agarose gel concentration is 1.5%).Among the figure: the M swimming lane: be dna molecular amount mark (250bp ladder).
Fig. 4: be the 279A-279G allelic mutation that pig INPP5F gene sequencing is found among the present invention.
Fig. 5: three kinds of genotype (the AA AB BB) electrophoretogram that is the HincII-RFLPs of pig INPP5F gene the 20 exon among the present invention.M:DNA molecular weight standard (250bp ladder)
Embodiment
The distribution situation of embodiment 1:PCR-RFLP-HincII polymorphism in each pig variety
Utilize PCR-HincII-RFLP to detect five pig kinds: the pig kind that wherein belongs to the place of china blood relationship is respectively the painted face in Beijing opera pig, the Arabescato pig, pig is deceived in Yushan, Jiangxi, the pig kind that belongs to external for example American-European blood relationship is respectively duroc and Large White, and these pig kinds contain the genotype of each pig kind substantially.The gene frequency of the pig kind that test is used is as shown in table 3, and the equal equipotential gene A of discovery local pig breed is preponderated, and the external equal equipotential gene of pig kind G preponderates.
Table 4INPP5F gene PCR product genotype frequency and gene frequency
The chi square test result such as the table 5 of genotype frequency between several kinds.x
2Check genotype frequency otherness discovery between five pig kinds, domestic pig and external pig exist significantly or utmost point significant difference.
The PCR-HincII-RFLP genotype distribution x of table 5INPP5F gene
2Assay
Annotate: shoulder motes
*Statement P＜0.05; Shoulder motes
*Statement P＜0.01
Embodiment 2:
The association analysis of pig mark property
Pig INPP5F gene the 20th exon HincII-RFLP pleomorphism site and part growth, reproductive trait are carried out association analysis, genotype detection result shows that the AA genotype has 113 in 236 individualities, the AB genotype has 98 individualities, the BB genotype has 25 (because indivedual data disappearances of measuring proterties, therefore the actual value that obtains is as shown in table 2), carrying out in the association analysis with the part producing proterties, the proterties of being analyzed has part growth traits and reproductive trait, and the proterties of being analyzed is 30-100Kg day weight gain and omnidistance day weight gain.
As seen from table, A allelotrope is the favourable mark of 30-100Kg day weight gain and omnidistance day weight gain, and AA genotype mark can be used for improving the selective marker of growth speed of pigs.
Table 2INPP5F gene pleiomorphism is to the influence of 30-100Kg day weight gain
Table 3INPP5F gene pleiomorphism is to the influence of omnidistance day weight gain
Sequence table
SEQUENCE?LISTING
＜110〉Hua Zhong Agriculture University
＜120〉clone of pig growth character gene INPP 5 F and application thereof
<130>
<141>2007-03-30
<160>1
<170>PatentIn?version?3.1
<210>1
<211>355
<212>DNA
＜213〉pig (Sus scrofa)
<220>
<221>gene
<222>(1)..(355)
<223>
<220>
<221>mutation
<222>(279)..(279)
<223>
<400>1
gactcctacc?actccgatga?attccttaca?aattccaagt?ctgatgaaga?taggcagcta 60
gccaactctt?tagagaatgt?agggccgata?gactatgttc?tgcctagttg?tggcattatt 120
gcttcagcac?ctcgtttagg?cagtcgatcc?cagtctatta?gcagcactga?tattagcatt 180
cacgttcctt?cagaggttcc?tactgctcat?ggaagtgggc?ttggaaaagg?ccacgagtct 240
tctttgaaga?aaagtccttc?tgctgacaac?atacacacrt?tgactggctt?tgccaagcct 300
gtggatgttt?actgccacag?atttgtccaa?gacgcacaaa?acaaagtgac?ccagc 355
Claims (6)
1, a kind of pig growth character gene INPP 5 F, its dna sequence dna is as described in the sequence table SEQ ID NO:1.
2, a kind of pig growth character gene INPP 5 F, its dna sequence dna wherein have the base mutation of an A279-G279 at the 279bp place of sequence table SEQ ID NO:1 shown in sequence table SEQ ID NO:1, cause Hinc II-RFLP polymorphism.
3, the described a kind of pig growth character gene INPP 5 F of claim 1, the dna sequence dna of forward and reverse primer of sudden change that wherein detects A279-G279 base place is as follows:
Forward: 5 ' GACTCCTACCACTCCGATGAAT 3 ',
Oppositely: 5 ' GCTGGGTCACTTTGTTTTGTG 3 '.
4, claim 1 or 2 application of described a kind of pig growth character gene INPP 5 F in the pig marker assisted selection.
5, the application of the primer of a kind of pig growth character gene INPP 5 F described in the claim 3 in the pig marker assisted selection.
6, a kind of screening pig growth character gene INPP 5 F molecular marker method, according to following steps:
Personnel selection INPP5F gene cDNA is the information probe, does the homologous sequence screening, obtains the expressed sequence tag of similarity more than 90%; Splice pig expressed sequence tag-contig then; According to expressed sequence tag-contig design primer, utilize primer to carry out pcr amplification, to PCR product purification, clone and the order-checking that obtains, carry out sequential analysis, obtain as the described dna sequence dna of sequence table SEQ IDNO:1.
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| CNB2007100517728A CN100529073C (en) | 2007-04-02 | 2007-04-02 | Clone and application for pig growth character gene INPP5F |
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| CN101343663B (en) * | 2008-05-09 | 2012-12-12 | 华中农业大学 | Molecular marker clone correlated with pig growth rate and application thereof |
| CN101348832B (en) * | 2008-07-16 | 2011-08-10 | 华中农业大学 | Clone and use of molecular marker related to pig growth and meat quality traits |
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Non-Patent Citations (2)
| Title |
|---|
| A Novel Variant of Inpp5f Is Imprinted in Brain, and ItsExpression Is Correlated with Differential Methylation of andInternal CpG Island. Jonathan D.Choi, Lara A. Underkoffler, Andrew J.Wood等.Molecular and Cellular Biology,Vol.25 No.13. 2005 |
| A Novel Variant of Inpp5f Is Imprinted in Brain, and ItsExpression Is Correlated with Differential Methylation of andInternal CpG Island. Jonathan D.Choi, Lara A. Underkoffler, Andrew J.Wood等.Molecular and Cellular Biology,Vol.25 No.13. 2005 * |
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