CN100515461C - Gamboge soft paste preparation - Google Patents
Gamboge soft paste preparation Download PDFInfo
- Publication number
- CN100515461C CN100515461C CNB2006101037155A CN200610103715A CN100515461C CN 100515461 C CN100515461 C CN 100515461C CN B2006101037155 A CNB2006101037155 A CN B2006101037155A CN 200610103715 A CN200610103715 A CN 200610103715A CN 100515461 C CN100515461 C CN 100515461C
- Authority
- CN
- China
- Prior art keywords
- gamboge
- resina garciniae
- cell
- extractum
- paraffin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to Chinese medicine preparation, and especially gamboge ointment and its preparation process. The gamboge ointment is prepared with gamboge, windpipe trachea, dandelion, semiaquilaia root, airpotato yam and other five kinds of Chinese medicinal materials in certain weight proportion. It is used in treating skin cancer, skin ulcer, innominate toxic swelling, etc. and has obvious curative effect and no toxic side effect.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation, specifically, is a kind of Gamboge soft paste preparation.
Background technology
Resina garciniae is a Chinese medicine simply, motherland's medical book record, and Resina garciniae has counteracting toxic substances, phagedenoma, and removing blood stasis eliminating stagnation etc. is attacked effect, and external treatment carbuncle, scrofula, stubborn dermatitis, malignant boil, overdose have saying of envelope larynx.The inquiry data at home and abroad does not see to be useful on skin ulcer, the treatment report of innominate toxic swelling, skin cancer etc.
Summary of the invention
The object of the present invention is to provide a kind of Gamboge soft paste preparation based on Resina garciniae.
For achieving the above object, the present invention adopts following technical scheme: it is made by the raw material of following parts by weight: Resina garciniae 40-50, seven leaves-branch flower 80-100, Fructus Gardeniae 80-100, Herba Taraxaci 80-100, Radix Semiaquilegiae 80-100, Rhizoma Dioscoreae Bulbiferae 80-100, Herba Polygalae Japonicae 80-100, vaseline 500-600, paraffin 60-80, Borneolum Syntheticum 15-20.
Preparation technology of the present invention is as follows:
A, Resina garciniae alcohol extraction concentrate extractum;
B, get Rhizoma Paridis, Fructus Gardeniae, Herba Taraxaci, Radix Semiaquilegiae, Rhizoma Dioscoreae Bulbiferae, Herba Polygalae Japonicae, decocting in water concentrate extractum;
C, above-mentioned two kinds of extractum mixings add vaseline, paraffin, Borneolum Syntheticum, stir and make ointment.
The inventor is from June, 1973, and, antiinflammatory anticancer to Resina garciniae carried out a series of research, and the scientific research through hardships decades has confirmed that the main anticancer effective component in the Resina garciniae is gamlogic acid, allogambogic acid, neogambogic acid.The inventor has not only broken through traditional usage that Resina garciniae cannot be taken orally to the research of Resina garciniae, and has received the special result of treatment cancer.Resina garciniae treatment cancer, its toxic and side effects is little than chemotherapeutics such as the 5-Fu of clinical use, camptothecine, cyclophosphamide, no hemogram inhibitory action.
Efficacy experiment
1, Resina garciniae antitumor action
Experiments such as nineteen eighty-two Lei Qiumo show that Resina garciniae is to mouse hydroperitoneum type hepatocarcinoma ECA, ARS, S
180, S
37, MA
737, U
14, ARA
4, W
256Inhibitory action is arranged, and suppression ratio is 35.6%～80.0%, the lethal effect that the human liver cancer cell BEL-7402 and the strain of HeLa cervical cancer cell of In vitro culture also had varying degree; The SMMC-7721 hepatoma cell strain is also had tangible lethal effect, and this lethal effect and cyclophosphamide are approximate, greater than 5-fluorouracil, less than amycin.The Cao Ji people in 1980 etc. once had obvious suppression and lethal effect to In vitro culture hepatoma cell strain SMMC-7721.Contrast experiment for index observes from cultured cell morphology damage, and Resina garciniae is eager to excel than the inhibitory action of amine salt, pretazettine in camptothecine, the lycorine.Liu Ruoyong etc. merge radiotherapy to Resina garciniae to be had potentiation, can improve mouse tumor MA
737Radiocurable effect.Cheng Tingkai etc. can cause S by the electron microscopic observation Resina garciniae
180Cell surface microvilli reduces, even disappears; Rough endoplasmic reticulum obviously reduces, and mitochondrion swells, gauffer, even breaks, and can make mice reticulum cell sarcoma ARA
4Ascites cells mitosis and amitosis index significantly reduce.Radiocolloid
198Gold does not have obvious enhancing or inhibition to the animal body immunologic function; It is synthetic that the isotope autoradiography points out this medicine to suppress ARS oncocyte DNA, influences the number of chemical composition of oncocyte, similar with alkylating agent, belongs to the cell cycle nonspecific drug.1984, usefulness flow cytometry (FCM) such as mound Xiang Huan in 1986 and precociously assemble the influence that the research of chromosome (PCC) method was divided a word with a hyphen at the end of a line to HeLa cell, Murine Ascitic Hepatoma Cells cycle also proves G
2It is the main mechanism that suppresses that → M delays.
2, gamlogic acid antitumor action
Use flow cytometry (FCM) and precocious dyeing (PCC) method of assembling is observed the influence that gamlogic acid was divided a word with a hyphen at the end of a line to HeLa cell and Murine Ascitic Hepatoma Cells cycle, found that 1.8 μ g/ml pair cells have obviously kills and wounds, and cell death is more than 20%.Mouse hydroperitoneum type hepatocarcinoma is had same inhibitory action during with 15mg/kg dosage, effect suppress for gamlogic acid can cause the Hela cell cycle each the time phase cell number change, G phase, S phase and the minimizing of M phase cell.And G
2The phase cell increases, and illustrates that medicine can suppress G
2The phase cell was divided a word with a hyphen at the end of a line to the M phase; Dong became the gamlogic acid effect 24hr with 5 μ g/ml in 1988, can effectively kill hepatoma carcinoma cell, and its antitumaous effect and drug level and action time are proportionate; Yellow Yongchang is reported the gamlogic acid lumbar injection S
180Ascitic type has the obvious suppression effect; But for avoiding the individual variation of tumor, select the not good mice transplantability net cell plastid sarcoma of energy for growth to carry out inhibition test, suppression ratio is respectively 32.9%, 38.1% and 40.1%, proves to have tumor-inhibiting action equally.During with H500 type scanning electron microscopic observation gamlogic acid intraperitoneal administration, the microvillus of ehrlich ascites cell is seen obvious minimizing or disappearance, mitochondrial swelling, and ultrastructure such as break; And ARA
4Cell mitogen and amitosis index significantly reduce, thereby point out this medicine may belong to a kind of cytotoxic drug.
3, neogambogic acid antitumor action
Experiment shows that neogambogic acid has that anticancer spectrum is wide, the low characteristics of toxicity.With the gamlogic acid contrast, neogambogic acid is to mouse leukemia L
1210Inhibitory action more excellent, prolongs the highest life cycle and can reach 292%, and the highest of gamlogic acid reaches 119%.Research to the ehrlich carcinoma curative effect has also shown effectively strong dependency of neogambogic acid, the increase that (ip) its trophophase prolongs with dosage in dosage 3.6mg/kg～7.0mg/kg scope increases, simultaneously administering mode with the next day, every 4 days or once heavy dose of administration ehrlich carcinoma all there is obvious curative effects, and than administration every day is excellent, for clinical application provides foundation.Neogambogic acid is to S simultaneously
180, Lewis lung cancer, Las
795Solid carcinomaes such as adenocarcinoma of lung all have better inhibitory action, and the effect of certain selectivity metastasis is arranged.Use micro ectrophotometry and scan method and studied neogambogic acid L
1210The influence in leukaemia's cycle, the result shows that neogambogic acid (10mg/kg) can make S phase cell reduce G
1The phase cytosis new cell peak occurs at low passage place.The appearance of these low DNA content cells has illustrated that neogambogic acid can suppress G
1The carrying out of～S phase cell; Also may be because this medicine has destroyed S phase cell.In addition, neogambogic acid (10mg/kg) is to L
1210The rna content of cell also has obvious attenuating effect.
4, other
Zhu Run weighing apparatus in 1984 waits once used 30% Resina garciniae tincture concentration 10
-7During g/ml to SM
44-RK
7Growth inhibitory action is arranged, think that tentatively Resina garciniae has direct inhibitory action to herpesvirus I type.Nanchang City Medical House microbe research chamber is measured the Resina garciniae antibacterial effect and is thought that gram-positive bacterium is had antibacterial action, and the antibacterial action to bacillus is stronger especially.
5, staining cell morphological observation
Mouse inoculation cancerous cell (ECA, S
180The ascitic type cell) after, with the treatment of gamlogic acid 5mg/kg amount, puts to death animal after continuous 7 days respectively, get the peritoneal exudate smear, establish normal control and cyclophosphamide (15mg/kg) positive control simultaneously.The matched group growth of cancer cells is vigorous, and RNA is abundant, and green one the Lip river uniform particles of first distributes, and cell is round bright-coloured, visible blue dark nuclear and thick red kernel, and division mostly is side plate shape, flower type mutually, divides the normal pseudopodium sample of phase endochylema projection also visible latter stage.Cyclophosphamide-a control group cancerous cell is similar to the normal control group, weakens but the cavity basophilia often appears in cancerous cell.The gamlogic acid experimental group does not find cancerous cell, sees a large amount of leukocyte.Mouse inoculation ECA cell, pneumoretroperitoneum administration in 6 days, morphocytology did not have much variations in 1,2 day, and after the administration in 3,4 days, cavity appears in visible cell, and the nuclear that has blurs, disappears or dissolving, and visible a large amount of leukocyte.
6, toxicity test
The ip in mice acute toxicity test, Resina garciniae LD
50Be 33mg/kg (fiducial limit 26-40); Gamlogic acid LD
50Be 6.2 ± 0.5mg/kg; Neogambogic acid LD
50Be 22.0 ± 2mg/kg.If it is heavy dose of medication can cause rabbit, rat, white mice organ disease, obvious with liver, kidney, lung especially; Low dose of accumulation medication, though dosage with last identical, pathological changes is significantly slight, limit to.Effective dose acts on Canis familiaris L., and the heart, liver, renal function do not have significant change, and only when adding 10 times of usual amounts, the electrocardiogram of rabbit has of short duration T ripple to shorten and the T ripple is little falls, so point out Resina garciniae to be used safely in clinical by the grasp dosage range.
7, internal metabolism
3The gamlogic acid of H labelling was still kept high concentration in 24 hours in the tumor body, and excretion is low, to organs and tissues selective gather in the acting body distribute higher with liver spleen content.Other is S
180The solid tumor autoradiography observation shows, is full of labeled drug at 5～7 hours in the hepatocellular endochylema, takes second place in the white pulp of spleen; A small amount of radioactive grain appears in the proximal convoluted tubule of 9 hours cardiac muscle fiber film places, kidney, and few in the lung; Radioactive grain after 24 hours in each organs and tissues does not increase.
The ointment that the present invention makes with Resina garciniae is used for cases such as clinical treatment skin carcinoma, skin ulcer, innominate toxic swelling, and is evident in efficacy, have no side effect, and be the motherland's Chinese medicine rarity that is worthy to be popularized simply.
The specific embodiment
Embodiment 1: it comprises the composition of following parts by weight: Resina garciniae 40, Rhizoma Paridis 80, Fructus Gardeniae 80, Herba Taraxaci 100, Radix Semiaquilegiae 100, Rhizoma Dioscoreae Bulbiferae 100, Herba Polygalae Japonicae 100, vaseline 500, paraffin 80, Borneolum Syntheticum 20.
Its preparation technology is as follows:
A, Resina garciniae alcohol extraction concentrate extractum;
B, get Rhizoma Paridis, Fructus Gardeniae, Herba Taraxaci, Radix Semiaquilegiae, Rhizoma Dioscoreae Bulbiferae, Herba Polygalae Japonicae, decocting in water concentrate extractum;
C, above-mentioned two kinds of extractum mixings add vaseline, paraffin, Borneolum Syntheticum, stir and make ointment.
Embodiment 2: it comprises the composition of following parts by weight: Resina garciniae 50, Rhizoma Paridis 100, Fructus Gardeniae 100, Herba Taraxaci 100, Radix Semiaquilegiae 100, Rhizoma Dioscoreae Bulbiferae 80, Herba Polygalae Japonicae 80, vaseline 600, paraffin 60, Borneolum Syntheticum 15.
Its preparation technology is as follows:
A, Resina garciniae alcohol extraction concentrate extractum;
B, get Rhizoma Paridis, Fructus Gardeniae, Herba Taraxaci, Radix Semiaquilegiae, Rhizoma Dioscoreae Bulbiferae, Herba Polygalae Japonicae, decocting in water concentrate extractum;
C, above-mentioned two kinds of extractum mixings add vaseline, paraffin, Borneolum Syntheticum, stir and make ointment.
Embodiment 3: it comprises the composition of following parts by weight: Resina garciniae 45, Rhizoma Paridis 90, Fructus Gardeniae 90, Herba Taraxaci 90, Radix Semiaquilegiae 90, Rhizoma Dioscoreae Bulbiferae 90, Herba Polygalae Japonicae 90, vaseline 550, paraffin 70, Borneolum Syntheticum 18.
Its preparation technology is as follows:
A, Resina garciniae alcohol extraction concentrate extractum;
B, get Rhizoma Paridis, Fructus Gardeniae, Herba Taraxaci, Radix Semiaquilegiae, Rhizoma Dioscoreae Bulbiferae, Herba Polygalae Japonicae, decocting in water concentrate extractum;
C, above-mentioned two kinds of extractum mixings add vaseline, paraffin, Borneolum Syntheticum, stir and make ointment.
Embodiment 4: it comprises the composition of following parts by weight: Resina garciniae 42, Rhizoma Paridis 85, Fructus Gardeniae 86, Herba Taraxaci 87, Radix Semiaquilegiae 90, Rhizoma Dioscoreae Bulbiferae 93, Herba Polygalae Japonicae 97, vaseline 580, paraffin 75, Borneolum Syntheticum 20.
Its preparation technology is as follows:
A, Resina garciniae alcohol extraction concentrate extractum;
B, get Rhizoma Paridis, Fructus Gardeniae, Herba Taraxaci, Radix Semiaquilegiae, Rhizoma Dioscoreae Bulbiferae, Herba Polygalae Japonicae, decocting in water concentrate extractum;
C, above-mentioned two kinds of extractum mixings add vaseline, paraffin, Borneolum Syntheticum, stir and make ointment.
Embodiment 5: it comprises the composition of following parts by weight: Resina garciniae 48, Rhizoma Paridis 98, Fructus Gardeniae 92, Herba Taraxaci 88, Radix Semiaquilegiae 86, Rhizoma Dioscoreae Bulbiferae 84, Herba Polygalae Japonicae 82, vaseline 530, paraffin 70, Borneolum Syntheticum 15.
Its preparation technology is as follows:
A, Resina garciniae alcohol extraction concentrate extractum;
B, get Rhizoma Paridis, Fructus Gardeniae, Herba Taraxaci, Radix Semiaquilegiae, Rhizoma Dioscoreae Bulbiferae, Herba Polygalae Japonicae, decocting in water concentrate extractum;
C, above-mentioned two kinds of extractum mixings add vaseline, paraffin, Borneolum Syntheticum, stir and make ointment.
Claims (1)
1, a kind of Gamboge soft paste preparation, it is made by the raw material of following parts by weight: Resina garciniae 40-50, Rhizoma Paridis 80-100, Fructus Gardeniae 80-100, Herba Taraxaci 80-100, Radix Semiaquilegiae 80-100, Rhizoma Dioscoreae Bulbiferae 80-100, Herba Polygalae Japonicae 80-100, vaseline 500-600, paraffin 60-80, Borneolum Syntheticum 15-20;
Its preparation technology is as follows:
A, Resina garciniae alcohol extraction concentrate extractum;
B, get Rhizoma Paridis, Fructus Gardeniae, Herba Taraxaci, Radix Semiaquilegiae, Rhizoma Dioscoreae Bulbiferae, Herba Polygalae Japonicae, decocting in water concentrate extractum;
C, above-mentioned two kinds of extractum mixings add vaseline, paraffin, Borneolum Syntheticum, stir and make ointment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101037155A CN100515461C (en) | 2006-07-27 | 2006-07-27 | Gamboge soft paste preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101037155A CN100515461C (en) | 2006-07-27 | 2006-07-27 | Gamboge soft paste preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1899484A CN1899484A (en) | 2007-01-24 |
| CN100515461C true CN100515461C (en) | 2009-07-22 |
Family
ID=37655595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006101037155A Expired - Fee Related CN100515461C (en) | 2006-07-27 | 2006-07-27 | Gamboge soft paste preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100515461C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112451617A (en) * | 2020-11-30 | 2021-03-09 | 江西藤康药业有限公司 | Gamboge ointment preparation and preparation process thereof |
-
2006
- 2006-07-27 CN CNB2006101037155A patent/CN100515461C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 中药藤黄制剂治疗41例皮肤癌. 林绍民.新医学. 1986 |
| 中药藤黄制剂治疗41例皮肤癌. 林绍民.新医学. 1986 * |
| 中药藤黄治疗皮肤癌13例临床观察. 宋奇思，衷诚任，刘定华，黄伟，陈俊.实用癌症杂志. 1986 |
| 中药藤黄治疗皮肤癌13例临床观察. 宋奇思，衷诚任，刘定华，黄伟，陈俊.实用癌症杂志. 1986 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1899484A (en) | 2007-01-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103768534A (en) | Traditional Chinese medicinal composition with anti-tumor activity | |
| CN102973608A (en) | Application of effective part of cockroach extract in preparing drug for inhibiting fungus growth | |
| CN101537036A (en) | Soap pod saponin extract as well as preparation method and application thereof | |
| CN1100063C (en) | Ginkgo Testa Polysaccharide and the preparation of pharmaceutical thereof | |
| CN100515461C (en) | Gamboge soft paste preparation | |
| CN1895337B (en) | Pharmaceutical use of Tangshen and Milkvetch root composition for improving life quality of late tumor patient | |
| CN115521385B (en) | Armillarisin mycelium polysaccharide, preparation method thereof and application thereof in resisting tumors | |
| CN1823787A (en) | Application of lucid ganoderma acid in preparation of cancer transfer inhibitor | |
| CN107095870B (en) | A kind of compound medicament composition and application thereof with anti-lymphadenoma effect | |
| CN1126539C (en) | Application of tanshinone in preparing medicine for treating tumor | |
| CN100515465C (en) | Gamboge preparation for treating various malignant tumors | |
| CN103330781A (en) | Traditional Chinese medicine composite with anti-tumor effect and preparation method of injection of same | |
| US20220000952A1 (en) | Method preparing antrodia cinnamomea extract and antrodia cinnamomea composition, and pharmaceutical composition | |
| CN1168457C (en) | Xiangshen injection and preparation process and use thereof | |
| CN100515453C (en) | Gamboge preparation | |
| CN1850149A (en) | Compounded preparation of Radix As tragali seu Hedy ari extract and mushroom polysaccharide and preparing method | |
| CN1709264A (en) | Use of ganoderic acid T and ganoderic and Me in tumour growth or proliferation inhibitor | |
| CN100367993C (en) | Gamboge paste for treating cervical erosion | |
| Chakravarty et al. | Two-way efficacy of alcoholic turmeric extract: Stimulatory for murine lymphocytes and inhibitory for fibrosarcoma cells | |
| US20230201294A1 (en) | Aqueous extraction process of plants, method thereof, and product by process | |
| CN103127296B (en) | Traditional Chinese medicine composition with lung cancer chemotherapeutic effect-enhancing and toxicity-reducing functions as well as preparation method and application thereof | |
| Xiao et al. | From Danggui Longhui Wan to meisoindigo: experience in the treatment of chronic myelogenous leukemia in China | |
| CN102416087A (en) | Chinese medicinal composition for treating pelvic inflammatory disease and preparation method as well as application thereof | |
| CN1081920C (en) | Herba pteridis semipinnatae extract and its medicinal composite | |
| CN1177592C (en) | Myocarditis treating medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090722 Termination date: 20180727 |