CN100509364C

CN100509364C - Method for preparing disposable plastic container for cultivating cell

Info

Publication number: CN100509364C
Application number: CNB2007101182844A
Authority: CN
Inventors: 陈凯
Original assignee: Individual
Current assignee: Individual
Priority date: 2007-07-04
Filing date: 2007-07-04
Publication date: 2009-07-08
Anticipated expiration: 2027-07-04
Also published as: CN101077630A

Abstract

The present invention relates to process of produce disposable plastic container for cell culture, and the plastic container is irradiated with ultrasonic ray to make the surface possess characteristic for cell to adhere for growth. The process includes preheating the ultrasonic ray source in wavelength range of 180-300 nm for 1-3 min, setting the container of plastic with molecular skeleton containing benzene ring structure in 1-10 cm below the ultraviolet lamp, controlling the humidity at 0-70 % and temperature at 20-60 deg.c, and irradiating for 5-20 min in the gas medium of air or oxygen. Thus produced disposable plastic container for cell culture has small contact angle and high hydrophilicity, and is suitable for cell to adhesion grow.

Description

The preparation method of the disposable plastic container of cultured cell

Technical field

The present invention relates to technical field of bioengineering, particularly the preparation method of the disposable plastic container of cultured cell.

Background technology

Disposable cell culture container (bottle, plate or ware) is that widely used cell of biomedicine field and tissue are cultivated consumptive material.Product with easy and simple to handle, efficient is high, no cross pollution is an advantage, is widely used in the aspect such as scientific research, teaching of biological medicine, universities and colleges, scientific research institution.This series products of China's use at present mainly is a dependence on import, or uses the glass wares of the need repeatability cleaning of easy cross pollution.

Plastics (polymer) are widely used as the container of cell Aseptic Culture, but have only the material through surperficial specially treated just to have the effect that makes cell attachment, division, growth and secretion bioactivator.Abroad, use the history that special treatment method is used for the cell culturing surfaces exploitation and produces surplus the series of products (Tissue Culture Flask, culture dish and culture plate) existing 30 of disposable cell culture container year.They adopt gas corona discharge and radio frequency plasma body technique that frosting is handled mostly, use these methods for the requirement of equipment than higher, fund input is relatively also bigger.

Summary of the invention

The object of the invention provides a kind of preparation method of disposable plastic container of cultured cell, and the inventive method is to handle frosting with ultraviolet irradiation technique, makes frosting have the characteristic that promotes cell attachment.

The inventive method mainly is that the ultraviolet ray of adopting quartzy low pressure mercury lamp to produce places the frosting that contains benzene ring structure in the molecular skeleton under the quartzy low pressure mercury lamp; Control relative air humidity and temperature; Be to carry out treatment with irradiation under the gas medium with air or oxygen.

Concrete steps of the present invention are:

Step 1: will produce the ultraviolet light source preheating 1-3min of wave-length coverage at 180nm-300nm; Step 2: the frosting that contains benzene ring structure in the molecular skeleton is placed 1-10cm place under the ultraviolet light source;

Step 3: gas relative humidity is controlled at .0%-70%;

Step 4: environment temperature is controlled at 20 ℃-60 ℃;

Step 5: irradiation time is controlled at 5-20min;

Step 6: the gas medium that adopts during irradiation is an air or oxygen.

Description of drawings

The zone that Fig. 1 handled for the inventive method and the contrast figure of untreated areas cell attachment growing state.

The specific embodiment

Embodiment 1:

With the quartzy low pressure mercury lamp of 40W, at the preheating 2min of pre-irradiation elder generation, the ultraviolet peak wavelength of generation is 184.9nm and 253.7nm; The polystyrene plastics bottle surface of unprocessed mistake is placed 5cm place under the light source; Relative air humidity is 60%; Environment temperature is 24 ℃; Irradiation time is 15min; Gas medium is an air.

Embodiment 2:

With the quartzy low pressure mercury lamp of 40W, at the preheating 2min of pre-irradiation elder generation, the ultraviolet peak wavelength of generation is 184.9nm and 253.7nm; The polystyrene plastics plate surface of unprocessed mistake is placed 5cm place under the light source; Relative air humidity is 20%; Environment temperature is 24 ℃; Irradiation time is 15min; Gas medium is an air.

Embodiment 3:

With the quartzy low pressure mercury lamp of 40W, at the preheating 2min of pre-irradiation elder generation, the ultraviolet peak wavelength of generation is 184.9nm and 253.7nm; The polystyrene plastics ware surface of unprocessed mistake is placed 5cm place under the light source; Relative air humidity is 20%; Environment temperature is 60 ℃; Irradiation time is 10min; Gas medium is an air.

Embodiment 4:

With the quartzy low pressure mercury lamp of 100W, at the preheating 3min of pre-irradiation elder generation, the ultraviolet peak wavelength of generation is 184.9nm and 253.7nm; The polystyrene plastics vessel surface of unprocessed mistake is placed 1cm place under the light source; Relative air humidity is 60%; Environment temperature is 24 ℃; Irradiation time is 10min; Gas medium is an air.

Embodiment 5:

With the quartzy low pressure mercury lamp of 100W, at the preheating 3min of pre-irradiation elder generation, the ultraviolet peak wavelength of generation is 184.9nm and 253.7nm; The polystyrene plastics surface of unprocessed mistake is placed 1cm place under the light source; Relative air humidity is 60%; Environment temperature is 24 ℃; Irradiation time is 12min; Gas medium is an air.

Embodiment 6:

With the quartzy low pressure mercury lamp of 40W, at the preheating 2min of pre-irradiation elder generation, the ultraviolet peak wavelength of generation is 184.9nm and 253.7nm; The polystyrene plastics bottle surface of unprocessed mistake is placed 10cm place under the light source; Relative air humidity is 10%; Environment temperature is 20 ℃; Irradiation time is 20min; Gas medium is an oxygen.

The advantage that the present invention has is:

1. because this method adopts ultraviolet irradiation technique to process frosting, it has establishes Standby require low, small investment, easy and simple to handle, promote the advantages such as the cell attachment effect is good, be suitable for Industrialization.

2. domestic enterprise and scientific research institution not yet grasp this frosting and process skill at present Art, the technology of the present invention can remedy blank in this respect, simultaneously, can form at home New industry improves China in this field integral level and efficient, has great society's effect Benefit also can be accelerated the research work of China biologic medical mechanism, universities and colleges, scientific research institution Progress, and can bring considerable economic benefit thus.

With market on main product Corning Tissue Culture Flask compare, such as table 1:

Table 1

Title	Material	Contact angle (mean value)	Remarks
Title	Material	Contact angle (mean value)	Remarks	The Corning tissue is cultivated the level surface	Polystyrene	55.75	The mainstream product in the market
Corning CellBIND surface	Polystyrene	13.4°	The optimum product of Corning, but price is slightly expensive	The Corning tissue is cultivated the level surface	Polystyrene	55.75	The mainstream product in the market
Corning CellBIND surface	Polystyrene	13.4°		This method treatment surface	Polystyrene	12.1°	The product that uses present technique to produce can be controlled in 50 % of price of external like products

As can be seen from Table 1, what contact angle reflected is the hydrophilic height of frosting, and contact angle more Little, hydrophily is more high, and hydrophilic height directly determines the cell attachment effect, and hydrophily is more high, The cell attachment effect is more good, and therefore, we can know from table 1: use technique to process The frosting contact angle is very low, and hydrophily is very high, is suitable for the cell attachment growth, the cell culture experiments card Real this result.

The product of the inventive method preparation, exploitation and operation all can be satisfied the product that increases rapidly both at home and abroad The product demand. Simultaneously, can form at home new industry, improve China and reach in this field integral level Efficient has great social benefit, also can accelerate China biologic medical mechanism, universities and colleges, section Grind the research work progress of mechanism, and bring therefrom considerable economic benefit.

Claims

1, a kind of preparation method of disposable plastic container of cultured cell is characterized in that:

Step 1: with ultraviolet light source is quartzy low pressure mercury lamp, and the ultraviolet peak wavelength that quartzy low pressure mercury lamp produces is the ultraviolet light source preheating 1-3min of 184.9nm and 253.7nm;

Step 2: the polystyrene plastics surface is placed 1-10cm place under the ultraviolet light source;

Step 3: gas relative humidity is controlled at 0%-70%;

Step 4: environment temperature is controlled at 20 ℃-60 ℃;

Step 5: irradiation time is controlled at 5-20min;

Step 6: the gas medium that adopts during irradiation is an air or oxygen.

2, the preparation method of the disposable plastic container of cultured cell as claimed in claim 1 is characterized in that: described disposable plastic cell culture container is bottle, plate or ware.