CN100503832C - Expression vector for promotor of casein gene of milch goats of 'guanzhong' - Google Patents

Expression vector for promotor of casein gene of milch goats of 'guanzhong' Download PDF

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CN100503832C
CN100503832C CNB2004100731643A CN200410073164A CN100503832C CN 100503832 C CN100503832 C CN 100503832C CN B2004100731643 A CNB2004100731643 A CN B2004100731643A CN 200410073164 A CN200410073164 A CN 200410073164A CN 100503832 C CN100503832 C CN 100503832C
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gene
milk
sequence
goat
promotor
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CN1661018A (en
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陈苏民
梁克明
童德文
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Fourth Military Medical University FMMU
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Abstract

An expression carrier configured by using the casein gene promoter domain of Guanzhong milk goat is disclosed. It features that its DNA sequence contains the promoter of beta-casein gene in milk gland tissue of said milk goat, its peripheral regulation sequence, the first intron, the first and the second extrons, and its signal peptide 6.5 Kb. It can be used to configure the milk gland bioreactor of transgenic goat for producing target protein.

Description

Central Shanxi Plain milk goat promotor of casein gene expression vector
Technical field
The invention belongs to technical field of bioengineering, relate to and a kind ofly can make target protein specific expressed justacrine expression vector in the goat milk in goat mammary gland with what clone milk goat promotor of casein gene district, the Central Shanxi Plain made up.
Background technology
(1) developing history of animal mammary gland bioreactor and present Research
1980, Gordon[1] adopt micro-injection method that recombinant DNA is imported mouse fertilized egg, obtained to have the transgenic mice of foreign gene first, this is that the mankind carry out engineered trial to mammiferous genetic information first.Nineteen eighty-two, Palmiter etc. [2] produce hormone gene with rat and are expelled in the mouse fertilized egg, have obtained " supermouse " more much bigger than common mouse first, and the imagination of the valuable pharmaceutical protein that proposes to purify from transgenic animal." supermouse " this fact that is born shows that the genetic manipulation of trafficability characteristic cell can import foreign gene in the mammiferous genome, and it is expressed effectively, thereby produces corresponding physiological effect.This result of study indicates the formation of brand-new research system one a transgenic animal system in the life science simultaneously.
Transgenic animal be considered to after genetics relaying gene linkage analysis, somatocyte heredity and the DNA reorganization the 4th generation modern biotechnology.Two ten years after " supermouse " is born, Study on Transgenic Animal has obtained development at full speed.At present, the transgenic mice technology of preparing is own through very ripe, and has obtained using widely at biological field, and huge pushing effect has been brought into play in the development of Biological Science and Technology.After the eighties, the animal transgenic technology is applied to other animals such as rabbit, poultry, goat, sheep, pig, ox gradually, oneself has tens of kinds of foreign genes to obtain to express in above-mentioned animal at present, come true from imagination as oneself warp of the factory's (claiming animal bioreactor again) that produces activated protein with transgenic animal, and demonstrate huge economic potential, be described as the gold industry of 21st century.
In animal bioreactor research, what progress was the fastest, DEVELOPMENT PROSPECT is best is animal mammary gland bioreactor.Its ultimate principle is to have goal gene and the fusion of milk protein gene expression regulation sequence that important exploitation is worth, and is built into mammary gland special (fixed point) expressing gene member, will obtain the transgenic animal offspring behind this gene component importing animal embryo.When these animal galactopoiesis, foreign gene obtains to express in mammary gland inducing under the guidance with the milk protein gene regulating and controlling sequence of prolactin antagonist, expression level can near or surpass the content of normal milk-protein.Therefore, the mammary gland of this transgenic animal as long as feed with feed to animal, just can obtain the valuable albumen of biologically active just as producing the natural factory of activated protein continuously from its milk.
Since recombinant DNA technology was come out, human oneself produced pharmaceutical protein through having set up many gene engineering expressions system.Though these expression systems all have its advantage separately, all can't compare with animal mammary gland bioreactor.What use was the widest at present is prokaryotic micro-organisms gene engineering expression system, though its technical process is simple relatively, the lead time is short, cost is lower, its technology is also quite ripe, and existing a considerable amount of gene engineering products promote the well-being of mankind, but because prokaryotic cell prokaryocyte can not effectively be processed eukaryotic protein, folding and modification, complicated eukaryotic gene expression product does not often have biological activity, or exist with insoluble inclusion body form, need could obtain useful products through complicated sex change and renaturation process.Studying at present and using the another kind of system of many genetically engineereds is the animal cell expression system, though its expression product is active higher, technology and conditional request harshness that cell large scale is cultivated, expression level is not high yet, cost an arm and a leg, so its actual application value is restricted.Mammary gland bioreactor of transgenic animals not only has the advantage of above-mentioned expression system, also can overcome their shortcoming and defect, mammiferous mammary gland expression system has the following advantages: (1) is not being damaged under the healthy situation of animal itself, easily a large amount of expression products of collecting goal gene from milk.(2) animal has higher fecundity, can make scale operation become possibility, and has the low advantage of production cost.(3) mammary epithelial cell has the ability of perfect translation post-treatment to expressed proteins, as glycosylation, phosphorylation and carboxylated etc., thereby makes expressed proteins have the function similar to native protein.(4) moiety of normal mammalian milk oneself study more clearlyly, the separation of being convenient to the destination gene expression product is like this purified.Protein content is 30～35 grams per liters in the Mammals milk, one cow head protein 10 00 gram of can giving milk every day, milk goat protein 20 0 gram of can giving milk, and the expression level of foreign gene in animal's mammary gland can near or surpass the content [3] of normal milk-protein.
Nineteen ninety, Dutch Pharming company (claiming Php again) cultivates the transgenic cattle of successful expressing human lactoferrin, contains human lactoferrin 1 gram in every liter of milk.Lactoferrin not only can promote the absorption of baby to iron, and can improve infant immunity, and the infection of opposing digestive tract diseases is the favorable substitutes of breast milk.3 transgenosis milk cows are produced 10 tons in milk per year, are worth 5,000,000,000 dollars.Recently, Dutch scientist has successfully cultivated the transgenic cattle that contains erythropoietin (EPO) again.1991, Edinburgh, Britain PPL drugmaker cultivated the Transgenic Sheep [4] of successful expression α 1 one antitrypsins (AAT).Have the activity that suppresses elastoser, be mainly used in treatment cystic fibrosis (CF) and pulmonary emphysema.AAT content in this Transgenic Sheep milk is up to 35 grams per liters, and therefrom the genetically engineered drug of Ti Chuning has passed through the II clinical trial phase, and enters American market.At present, the said firm has cultivated more than 200 of the transgenic sheep that produces AAT, and the target product of expressing with transgenic sheep and bovine mammary gland bio-reactor is nearly 20 kinds.The U.S. Genzyme Transgene company (GTC) and the SomitomoMetals of Japan cooperate, common its leading product thrombogen III of exploitation, the expression amount of transgenic goat Ruzhong recombinant products is 4g/L, has entered clinical trial [3] at present, and the portion gene product sees Table one.
Table one is the separation and purification target protein from transgenic animal milk liquid
Target protein Purification technique Transgenic animal The purifying characteristics Data source
Alpha1 Anti-trypsin Double-aqueous phase system distributes Transgenic Sheep Be applicable to that extensive purification requires target protein concentration height, Harris etc. [15]
Plasma thromboplastin component Immunoaffinity chromatography Transgenic Sheep Specificity height, but productive rate lower (2.0%～2.5%) Clark etc. [13]
Alpha1 Anti-trypsin Ion talk chromatography, dye affinity chromatography, gel-filtration Transgenic Sheep Unite multiple chromatography method product purity〉95% Wright or the like [2]
The Profibrinolysin active factor Immunoaffinity chromatography, hydrophobic chromatography Transgenic goat Specificity is good, the purity high yield〉25%, purity〉98 Denman etc. [16]
Activated protein C Immunity is affine, and layer is rolled over Transgenic pig Specificity is good Van Cott [17]
Activated protein C Immobilized metal affinity chromatography Transgenic animal Efficiently, rapid Dalton
Blood coagulation factor VIII The immunobead absorption method Transgenic pig The specificity height Paleya nda
Plasma thromboplastin component and PROTEIN C Zn (2+)-selective precipitation method Transgenic animal The product purity height〉95% Degene r
[13]Clark A J，Bessos H，Bishop J O et al.Bio/Technology，1989，7：487-492
[14]Wright G，Binicda A，Udell M.J Chem Tech Biotechnol，1994，59：1I0
[15]Harris D P，Andrens A T，Wright G et al.Bioseparation，1997，7(1)：31-37
[16]Denman J，Hayes M，Oday C et al.Bio/Technolagy，1991，9：839～843
[17]Van Cott K E，Williams B，Velander W H et al.J Mol Recagnit，1996，9(5～6)：407～414
[18]Dalton J C，Bruley D F，Kang K A et al.Ado Exp Med Biol，1997，411：419～428
[19]Degener A，Belew M，Velander W H et al.J
Figure C200410073164D0013153908QIETU
，I998，13：125～137
[2]Wright G，Carver A，Cotto D et al.Bio/Technology.1991，9：830～834
[10]Paleyanda R K，Velander W H，Lee T K et al.Nat Biotechnol，1997，15：97I～975
Some companies of countries such as Britain, the U.S., Holland utilize bio-reactor to produce anticoagulation III, human lactoferrin, EPO, Interferon, rabbit etc. and have also entered the clinical experiment stage.Britain Roslyn institute utilizes transgenic sheep, produces the emophysematous haemophiliachemophiliac goat milk of treatment in batches.China has obtained to change human serum albumin, Regular Insulin, the isogenic ox of Interferon, rabbit, sheep and rabbit.
The professor Li Ning of China Agricultural University etc. adopt microinjection that external source people MAAT (modifier antitrypsin) gene is imported in the goat pronuclear-stage embryos, plan is from the milk of goat, extract people's pharmaceutical protein, research and development contain people keep healthy proteic dietetic product and biologics.The relevant expert thinks, obtains the people's gene transgenic sheep, indicates that China's transgenic technology has entered the new stage, has explored another new approach for utilizing animal mammary gland bioreactor to produce biologics.External economist's prediction, after about 10 years, the biological products that transgenic animal produce will the legs of the tripod in the world market, singly be that the annual sales amount of medicine just will be above 25,000,000,000 dollars.
(2) gene regulating of mammary specific expression research
The structure of animal's mammary gland specific expression carrier and the height of expression level thereof depend on the understanding to milk protein gene expression regulation mechanism to a great extent.At present, cloned many milk protein genes, oneself is delivered the nuclear former times acid of part milk protein gene and regulating and controlling sequence thereof.The controlling element that is used for the galactophore of transgenic animal localization and expression mainly contains following four classes:
The first kind: β-lactoglobulin (BLG) gene regulating element.Simons etc. change the BLG gene of sheep over to mouse, and β-lactoglobulin of sheep is specific expressed in mouse mammary gland. and content can reach 23g/L[8 in its milk liquid].
Second class: casein gene regulating and controlling sequence.The regulating and controlling sequence of cow commonly used and sheep beta-casein gene.As αS1-Lao Danbaijiyin regulating and controlling sequence Conrad interleukin-2 gene successful expression [9] in transgene rabbit milk liquid.
The 3rd class: whey acid protein (WAP) gene regulating sequence.WAP is the main protein in the rodent milk liquid, does not have the existence of WAP in domestic animal milk liquid. but WAP gene regulating sequence can instruct foreign gene to express [10] in domestic animal milk liquid.
The 4th class: whey albumin gene regulating sequence.
Although the content difference of milk-protein in the different animals milk, but what content was the highest all is casein, beta-casein accounts for more than 37% and 50% of total milk protein (Provot et al 1995) respectively in milk and goat milk, show that the beta-casein promoter activity is very strong, it can start foreign gene and express (vi-vito) in the mammary tissue of transgenic animal.Therefore casein gene is a main research object.
[1] Gordon K.et al.Genetic transformation of mouse embryos by microinjection of purifiedDNA.PNAS.1980,77:7380 1
[2]Palmiter RD et al.Dramatic growth of mice that develop from eggs microin-jected withmetallothionein-growth hormone fusiongenes Nature.1982，300：611
[3] present Research of Jing Lunlu scholar who combines profoundity with virtue Xue galactophore biological reactor, biotechnology circular .1998,3:17 1
[4]Wright G，Carver A et al.High level expression of active human alpha-l-antitrypsin inthe milk of transgenic sheep.Bio/Technology.1991，9：830-834
[8]Simons J P，Mccienaghan M，Clark A J.Nature，1987，328：530～532
[9]Buhler T，Bruyere T B，Went D F et al.Bio/Technology，1990，8：140～143
[10]Paleyanda R K，Velander W H，Lee T K et al.Nat Biotechnol，1997，15：97I～975
(3), technology related to the present invention
Professor Ceng Yitao of Shanghai Inst. of Medical Genetics, Shanghai city Children's Hospital leader's study group is at Acta Genetica Sinica 29 (3); 206-211,2002 deliver about in " the transgenic mice milk that goat beta-casein gene promotor instructs efficiently expresses human blood coagulation IX " literary composition, use beta-casein gene promotor 6.7kb and instruct exogenous gene expression, its constructed goat beta-casein gene promoter sequence has also kept upstream regulatory region and beta-casein first intron, first exon and second exon outside keeping original near-end composition.Carry out the secretion expression but target protein is inserted carrier, its effect is undesirable, and cause and effect all needs through test again.
Summary of the invention
At above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of expression vector of originating as the beta-casein gene promoter sequence with the mammary tissue of the distinctive high yield milk goat of northwest Yang Ling, this expression vector can be used in sets up the transgenic goat galactophore biological reactor, use that the constructed target protein of genetic engineering means is expressed specifically in goat mammary gland, justacrine in goat milk, produce target protein for preparation.
The Central Shanxi Plain of the present invention milk goat promotor of casein gene expression vector, have the merchant and sell the beta-casein gene promoter region that plasmid vector and beta-casein gene promotor and regulating and controlling sequence on every side and first intron, first exon and second exon thereof are constituted, it is characterized in that:
(1), described beta-casein gene promoter region sequence also is included in the terminal restriction endonuclease Sgf I site sequence of introducing as signal peptide of second exon; This starts regional sequence and is-4359～+ 2106bp (basepair), total length 6465bp;
(2), described beta-casein gene promotor is that mammary tissue with the distinctive high yield of northwest Yang Ling Central Shanxi Plain milk goat is as the source, therefrom extract genomic dna as template, the design synthetic primer, archaeal dna polymerase with high-fidelity carries out polymerase chain reaction (PCR), obtain the PCR product, get through splicing, clone and mensuration sequence;
(3), described Central Shanxi Plain milk goat promotor of casein gene expression vector is to start regional sequence 6465bp and insert in the plasmid vector that the merchant sells by restriction enzyme and get (seeing below) containing Central Shanxi Plain milk goat beta-casein gene.
The Central Shanxi Plain of the present invention milk goat beta-casein promotor detects through experiment, proves to have promotor and enhanser activity.Test experience and result are as follows:
1, take promotor and enhanser (the promoter and enhancer trap) technology of catching to detect:
The luciferase reporter gene carrier pGL3-Basic Vector (its ring junction composition is seen Fig. 3) that method is to use the merchant of Promega company to sell, the promoter sequence of 6465bp of the present invention is inserted between its polyclone restriction endonuclease sites KpnI and the BglII, be built into the plasmid pGL3-B65 that contains the 6465bp promoter sequence, the former goat mammary gland epithelial cell of being commissioned to train foster of transient transfection, induce down at prolactin (prolactin), the resultant quantity of reporter gene-luciferase significantly increases.Experimental data is as follows:
Figure C200410073164D00161
Experimental result proves absolutely that the 6465bp dna sequence dna of being cloned has promotor and enhanser activity.
2, take promotor (promoter trap) technology of catching to detect:
The luciferase reporter gene carrier pGL3-Enhancer Vector (its ring junction composition is seen accompanying drawing 4) that method is to use the merchant of Promega company to sell, the promoter sequence of 6465bp of the present invention is inserted between its polyclone restriction endonuclease sites KpnI and the BglII, be built into the plasmid pGL3-E65 of the promoter sequence that contains 6465bp, the former goat mammary gland epithelial cell of being commissioned to train foster of transient transfection, induce down at prolactin (prolactin), the resultant quantity of reporter gene-luciferase significantly increases.Experimental data is as follows:
Figure C200410073164D00171
Above-mentioned 2 groups of data statistics test of significance P＜0.05.Experimental result shows: the 6465bp dna sequence dna of being cloned has promoter activity.
Description of drawings
Fig. 1 is a beta-casein promoter region dna sequence dna synoptic diagram
Fig. 2 is that the merchant of Clontech company sells plasmid pcDNA3.1 (+/-) structural representation
Fig. 3 is that the merchant of Promega company sells plasmid pGL3-Basic Vector structural representation
Fig. 4 is that the merchant of Promega company sells plasmid pGL3-Enhancer Vector structural representation
Wherein:
Fig. 1: milch goat mammary gland beta-casein gene promoter region, the Central Shanxi Plain sequence of structure that the present invention clones comprises the promoter region of β-casein gene upstream, first, second extron and the First Intron of coding mammary gland beta-casein gene, and at the terminal restriction endonuclease Sgf I site sequence of introducing of Second Exon, be beneficial to insertion and the expression of external source genes of interest.
Among Fig. 2: Pcmv is the CMV promoter; BGH PA is the polyadenylic acid sequence of bovine growth hormone gene; Flori is dna single chain origin of replication; SV40ori is SV40 virus replication starting point; Neomycin is neomycin resistance gene; Ampicillin is ampicillin resistance gene; SV40pA is SV40 virus polyadenylic acid sequence; PUCori is pUC plasmid origin of replication; T7 Nhe1 ... Pme1 is many restriction endonuclease sites sequence, is the position that foreign DNA inserts. The present invention is with the foreign DNA insertion position T7 Nhe1 among the beta-casein gene promoter region sequence 6465bp insertion pcDNA3.1 (-) that clones ... the many restriction endonuclease sites of Pme1, namely obtain a kind of Central Shanxi Plain milch goat promotor of casein gene expression vector of wanting required for the present invention, between restriction endonuclease sites XhoI and BamHI, be built into carrier for expression of eukaryon.
Among Fig. 3: AmprBe the parasiticin resistant gene; Flori is dna single chain origin of replication; Synthetic poly (A) signal/transcriptional pause site is polyadenylic acid composite signal/transcribe terminating point, and this sequence can reduce background; KpnI.....HindIII is many restriction endonuclease sites sequence, inserts dna sequence dna to be detected at this; Luc+ is luciferase gene, is a kind of reporter gene; SV40 late poly (A) signal is the polyadenylic acid composite signal of SV40 virus late transcription unit; SalI, BamHI, HpaI, XbaI, NarI, NcoI are respectively the sites of different restriction endonuclease, and show the sequence location in its site.
Among Fig. 4: SV40 Enhancer is the SV40 enhancer sequence; AmprBe the parasiticin resistant gene; Flori is dna single chain origin of replication; Synthetic poly (A) signal/transcriptional pause site is polyadenylic acid composite signal/transcribe terminating point, and this sequence can reduce background; KpnI.....HindIII is many restriction endonuclease sites sequence, inserts dna sequence dna to be detected at this; Luc+ is luciferase gene, is a kind of reporter gene; SV40 late poly (A) signal is the polyadenylic acid composite signal of SV40 virus late transcription unit; SalI, BamHI, HpaI, XbaI, NarI, NcoI are respectively the sites of different restriction endonuclease, and show the sequence location in its site.
Embodiment
Embodiment 1:
Selling plasmid pcDNA3.1 (-) with the merchant of Clontech company is example, in conjunction with Fig. 2, the Central Shanxi Plain of the present invention milk goat promotor of casein gene expression vector is further described:
From the milk goat casein gene of the Central Shanxi Plain, extract genomic dna as template, the design synthetic primer, carry out polymerase chain reaction (being called for short PCR) amplification with the high archaeal dna polymerase of protecting of sequence, the PCR product that obtains, the Central Shanxi Plain milk goat beta-casein gene-4359-+2106bp that obtains through splicing, clone and mensuration sequence starts regional sequence (total length 6465bp) again; The merchant that the sequence of total length 6465bp is inserted Clontech company sells between the foreign DNA restriction endonuclease sites XhoI and BamHI among the plasmid pcDNA3.1 (-), obtains a kind of Central Shanxi Plain milk goat promotor of casein gene expression vector of wanting required for the present invention; The merchant of Clontech company sells polyadenylic acid sequence, Neomycin neomycin resistance gene, Ampicillin ampicillin resistance gene, the SV40pA virus polyadenylic acid sequence that plasmid pcDNA3.1 (-) plasmid contains Pcmv CMV promotor, BGHPA bovine growth hormone gene, its dna sequence dna total length is 5427bp
Embodiment 2:
Selling pGL3-Basic Vector plasmid with the merchant of Promega company is example, in conjunction with Fig. 3, the Central Shanxi Plain of the present invention milk goat promotor of casein gene expression vector is further described:
From the milk goat casein gene of the Central Shanxi Plain, extract genomic dna as template, the design synthetic primer, archaeal dna polymerase with the sequence high-fidelity carries out polymerase chain reaction (being called for short PCR) amplification, the PCR product that obtains, the Central Shanxi Plain milk goat beta-casein gene-4359-+2106bp that obtains through splicing, clone and mensuration sequence starts regional sequence (total length 6465bp) again; The merchant that the sequence of total length 6465bp is inserted Promega company sells in the pGL3-BasicVector plasmid between the KpnI and the many restriction endonuclease sites of BglII, obtains the another kind of Central Shanxi Plain milk goat promotor of casein gene expression vector of wanting required for the present invention; The merchant of Promega company sells in the pGL3-Basic Vector plasmid contains Amp rPenicillin G resistant gene, Luc+ are luciferase gene, the unitary polyadenylic acid composite signal of SV40 virus late transcription.
Embodiment 3:
Selling pGL3-Enhancer Vector plasmid with the merchant of Promega company is example, in conjunction with Fig. 4, the Central Shanxi Plain of the present invention milk goat promotor of casein gene expression vector is further described:
From the milk goat casein gene of the Central Shanxi Plain, extract genomic dna as template, the design synthetic primer, archaeal dna polymerase with the sequence high-fidelity carries out polymerase chain reaction (being called for short PCR) amplification, the PCR product that obtains, again through splicing, clone with measure sequence and the Central Shanxi Plain milk goat beta-casein gene-4359 that obtains～+ 2106bp starts regional sequence (total length 6465bp); The merchant that the sequence of total length 6465bp is inserted Promega company sells in the pGL3-EnhancerVector plasmid between the KpnI and the many restriction endonuclease sites of BglII, obtains the another kind of Central Shanxi Plain milk goat promotor of casein gene expression vector of wanting required for the present invention; The merchant of Promega company sells in the pGL3-Basic Vector plasmid contains Amp rPenicillin G resistant gene, Luc+ are luciferase gene, the unitary polyadenylic acid composite signal of SV40 virus late transcription, SV40 virus enhancer gene order.
It is as follows that Central Shanxi Plain milk goat beta-casein gene starts regional sequence total length 6465bp:
1 AGATGATTTT GCAACCCCCT GCCTCAGGAG ACACTGGGAA ATTTCCTGAG ACATTTTTGA
TCTACTAAAA CGTTGGGGGA CGGAGTCCTC TGTGACCCTT TAAAGGACTC TGTAAAAACT
61 TTCCAAAAGC TGTGCAGTTG GTGCTTCTAC CATCTTCGTG GTAGAGGTCA AGGATGCTGC
AAGGTTTTCG ACACGTCAAC CACGAAGATG GTAGAAGCAC CATCTCCAGT TCCTACGACG
121 TAAACATTCT ACAACACATT AAGAAAACCC CCACAACAAA GAATTCTTCC GCCAAAAATA
ATTTGTAAGA TGTTGTGTAA TTCTTTTGGG GGTGTTGTTT CTTAAGAAGG CGGTTTTTAT
181 TCAATAATAT GAAGGTTGAA AAATACTGGT CTAGCATGTA GTATGTGCTC AATAGCAAGG
AGTTATTATA CTTCCAACTT TTTATGACCA GATCGTACAT CATACACGAG TTATCGTTCC
241 AGAGAAAAGA AAGCCTTCCT CACTGATTAA TGCAAAGAAA TAGAGGAAAA CAATAGAATG
TCTCTTTTCT TTCGGAAGGA GTGACTAATT ACGTTTCTTT ATCTCCTTTT GTTATCTTAC
301 GGAAAGACTA GAGAGCTCTT CAAGCAAATT AGAGATATCA AGGGAACATT TCACGCAAAG
CCTTTCTGAT CTCTCGAGAA GTTCGTTTAA TCTCTATAGT TCCCTTGTAA AGTGCGTTTC
361 ATGGGCACAA TAAAGGACAG AAATTTTATG GAGGAGTTGC TGATGGAGAG GGAGGCCTGG
TACCCGTGTT ATTTCCTGTC TTTAAAATAC CTCCTCAACG ACTACCTCTC CCTCCGGACC
421 CGTGCTGCGA TTCCTGGGGT CGCAAAGAGT CGGACACAAC TGAGCGACTG AATTGAACTG
GCACGACGCT AAGGACCCCA GCGTTTCTCA GCCTGTGTTG ACTCGCTGAC TTAACTTGAC
481 AACTGAACTG GACAAAGCAG AAGATATTAA GAAGAGGTGG TAAGAATACA CAGAAGAACA
TTGACTTGAC CTGTTTCGTC TTCTATAATT CTTCTCCACC ATTCTTATGT GTCTTCTTGT
541 ATATAAAAAA GATCTTCATG ACCCAGATAA CCACGATGAT GTGATCACTC ACCTAGAGCC
TATATTTTTT CTAGAAGTAC TGGGTCTATT GGTGCTACTA CACTAGTGAG TGGATCTCGG
601 AGACACCCTG GAATGCAAAG TCAAACGGCC TTAGAAAGCC TCACTATGAA CAAAGCTAGT
TCTGTGGGAC CTTACGTTTC AGTTTGCCGG AATCTTTCGG AGTGATACTT GTTTCGATCA
661 GGAGGTAATG GAATTCCAGT TGAGCTATTT CAAATCTTAA AAGGTGATGC TGTGAAAGTG
CCTCCATTAC CTTAAGGTCA ACTCGATAAA GTTTAGAATT TTCCACTACG ACACTTTCAC
721 CTGCACTCAA TATGTCAGCA AATTTGGAAA ACTCAGCAGT GGCCACAGGA CTGCCACAAT
GACGTGAGTT ATACAGTCGT TTAAACCTTT TGAGTCGTCA CCGGTGTCCT GACGGTGTTA
781 CCCAAAGAAA AGCAATGACA AAGAATGTTC AAACACCCAC ATGATTGCAC TCATCTCACA
GGGTTTCTTT TCGTTACTGT TTCTTACAAG TTTGTGGGTG TACTAACGTG AGTAGAGTGT
841 TGCTAGCAAA ATAACTCTCA AAATTCTCCA AGCCAGGCTC CAACAGTACG TGGACCATGA
ACGATCGTTT TATTGAGAGT TTTAAGAGGT TCGGTCCGAG GTTGTCATGC ACCTGGTACT
901 ACTTCCAGAT GTTCAAGCTG GATTTAGAAA AGGCAGAGGA ACCAGAGATC AAATTGCCAA
TGAAGGTCTA CAAGTTCGAC CTAAATCTTT TCCGTCTCCT TGGTCTCTAG TTTAACGGTT
961 CATCCATTGG ATCATCAAAA AAGCACGAGA GTTCCAGAAA AACATCTGCT TTATTGACTA
GTAGGTAACC TAGTAGTTTT TTCGTGCTCT CAAGGTCTTT TTGTAGACGA AATAACTGAT
1021 CGCTAAAGCC TTTGATTGTG TGGATCACAA TAAACTGTGG AAAATTCTTC AAGAGATGGG
GCGATTTCGG AAACTAACAC ACCTAGTGTT ATTTGACACC TTTTAAGAAG TTCTCTACCC
1081 AATACCAGAC CACTTTACCT GCCTCCTGAG AAATCTGTAT ACAGGTCCAG AAGCAGCAGT
TTATGGTCTG GTGAAATGGA CGGAGGACTC TTTAGACATA TGTCCAGGTC TTCGTCGTCA
1141 TAGAACTGGA CATGGAACAA CAGACTGGTT CCAAACTGCG AAAGGGGTAC ATCAAGGAAT
ATCTTGACCT GTACCTTGTT GTCTGACCAA GGTTTGACGC TTTCCCCATG TAGTTCCTTA
1201 ATTCATTGGA AGGATTGATG CTGAAGCTGA AACTCCTATA CTTTGGCCAC CTAATGTGAA
TAAGTAACCT TCCTAACTAC GACTTCGACT TTGAGGATAT GAAACCGGTG GATTACACTT
1261 GATCTGACTC ATTGGAAAAG ACTCCAATGC TGGGAAAGAT TGAAGGCAGG AGAAGAGGAT
CTAGACTGAG TAACCTTTTC TGAGGTTACG ACCCTTTCTA ACTTCCGTCC TCTTCTCCTA
1321 GACAGAGGAT GAGATGGTTG GATGGGATCA CTGACTCAAT GGACATGAGT TTGAGTAAGC
CTGTCTCCTA CTCTACCAAC CTACCCTAGT GACTGAGTTA CCTGTACTCA AACTCATTCG
1381 TCCAGGGGTT GGTGGTGGAC AGGAAAGCCT GGCGTGCTGC AGTCCACAAG GTCACAAAGA
AGGTCCCCAA CCACCACCTG TCCTTTCGGA CCGCACGACG TCAGGTGTTC CAGTGTTTCT
1441 TTCGGACATG ACTGAGTGAC TGAACTGATA CTGATGTGCT CAACAAATGT ATCTTGAACT
AAGCCTGTAC TGACTCACTG ACTTGACTAT GACTACACGA GTTGTTTACA TAGAACTTGA
1501 TGTGTGAAGT TCTATGGTCA CATGTAAAGG AAGAATAATC AGGATTAGCT GTGTGTCTTA
ACACACTTCA AGATACCAGT GTACATTTCC TTCTTATTAG TCCTAATCGA CACACAGAAT
1561 GGAATCAGGG TTCTGAGTTT TATGTGTTCA TAGTATCTGC TGGTTCACAA AACATTTTTC
CCTTAGTCCC AAGACTCAAA ATACACAAGT ATCATAGACG ACCAAGTGTT TTGTAAAAAG
1621 TTATTCTCTG GTTCTTGATT TACTTTATAA AGTAATCTTA ATAGTTATAC TTCACATAGA
AATAAGAGAC CAAGAACTAA ATGAAATATT TCATTAGAAT TATCAATATG AAGTGTATCT
1681 TACGAAATTA TTATATTTGG ATAATCTCAT GGAAAGGATT AAATACTCCA TCTATTACGA
ATGCTTTAAT AATATAAACC TATTAGAGTA CCTTTCCTAA TTTATGAGGT AGATAATGCT
1741 GTAATGCTGA ACTATCTACT CCTACCTAAT AATTTGTCAG AATTCACTAA TTCTGTGTTA
CATTACGACT TGATAGATGA GGATGGATTA TTAAACAGTC TTAAGTGATT AAGACACAAT
1801 TATTGTTTCT AAATCTGAAT CATTATATGA ATCCTCAGTA TTTTGTTTTC CTTCCTCTAT
ATAACAAAGA TTTAGACTTA GTAATATACT TAGGAGTCAT AAAACAAAAG GAAGGAGATA
1861 ATTTTGGAAT TTATTAAACA GTGCTTCAAA TAATTTTTAG GAAACTGAAG TTTTTAGTAA
TAAAACCTTA AATAATTTGT CACGAAGTTT ATTAAAAATC CTTTGACTTC AAAAATCATT
1921 CAGCTCTATC TCTAAATAGC TTTAGTATCT TGAAAAAGTA ATACAAATTC TCACATCCTT
GTCGAGATAG AGATTTATCG AAATCATAGA ACTTTTTCAT TATGTTTAAG AGTGTAGGAA
1981 AATTTCCTCT TCTCTAAAAT ATCTTTAAAA TATTCTATGA ATGATATCTC TTAATATTTA
TTAAAGGAGA AGAGATTTTA TAGAAATTTT ATAAGATACT TACTATAGAG AATTATAAAT
2041 TTTTTTTGGC AATCCAACAC AGCTTATGGG ATCTTAGTTC CCCAGTGAGG GATTATATCC
AAAAAAACCG TTAGGTTGTG TCGAATACCC TAGAATCAAG GGGTCACTCC CTAATATAGG
2101 ATGCCAACTG CAGTGAAAGT ACAAAATCCT AAACTGGACT CACCAGGGAT TTCCCAATAT
TACGGTTGAC GTCACTTTCA TGTTTTAGGA TTTGACCTGA GTGGTCCCTA AAGGGTTATA
2161 CTCCTCTAGT TCTTATTTCT GAATATTTTT GGTCCCTTTA TTGTACTCTT CATCCAACTT
GAGGAGATCA AGAATAAAGA CTTATAAAAA CCAGGGAAAT AACATGAGAA GTAGGTTGAA
2221 TTCTATTGAT TTCTTTCTTG AGGTTATTAT TTACTTGGTT TCAGTTAGAA ATATATGCAA
AAGATAACTA AAGAAAGAAC TCCAATAATA AATGAACCAA AGTCAATCTT TATATACGTT
2281 ATCTCAGGAC TGCATATTTC AGATTCATTG GCCAATATGG GAAAAAACCT TTGGCTGAAC
TAGAGTCCTG ACGTATAAAG TCTAAGTAAC CGGTTATACC CTTTTTTGGA AACCGACTTG
2341 AAATCATGCT TATAAAAAAT AGTACTAGAG CATCCTACTT TGACTATATC TTGCTCCTCA
TTTAGTACGA ATATTTTTTA TCATGATCTC GTAGGATGAA ACTGATATAG AACGAGGAGT
2401 TTCAGGGTTA TCTAATACAA TTTCCCCACA TGAAATTCTT TTGCATTATA AAAATGGAAG
AAGTCCCAAT AGATTATGTT AAAGGGGTGT ACTTTAAGAA AACGTAATAT TTTTACCTTC
2461 CTCTTAGGTA ACATTGCAAA AATTCGAGTT GCTCATATGG CACTTTGCTT CTTACTGGTC
GAGAATCCAT TGTAACGTTT TTAAGCTCAA CGAGTATACC GTGAAACGAA GAATGACCAG
2521 ATTGTGTTCT GAGGCTTACC TGGACAGGTG GTACCTGATG TCATCTTAAA TTGCTGGCTT
TAACACAAGA CTCCGAATGG ACCTGTCCAC CATGGACTAC AGTAGAATTT AACGACCGAA
2581 TTTGATTTTC CATTGGACAA GCTTCTTTCT TTAGTATATT GTTAAGGATT TCCTTGATCA
AAACTAAAAG GTAACCTGTT CGAAGAAAGA AATCATATAA CAATTCCTAA AGGAACTAGT
2641 AGATTTTACC TACTTTTCTG GTCCAATTGG TGAGAGACAG TCATAAGGAA ATGCTGTGTT
TCTAAAATGG ATGAAAAGAC CAGGTTAACC ACTCTCTGTC AGTATTCCTT TACGACACAA
2701 TATTGCACAA TATGTAAAGC ATCTTCCTGA GAAAATAAAA GGGAAATGTT GAATGGGAAG
ATAACGTGTT ATACATTTCG TAGAAGGACT CTTTTATTTT CCCTTTACAA CTTACCCTTC
2761 GATATGCTTT CTTTTGTATT CCTTTTCTGA GAAATCAAAC TTTTTCACCT GTGGCCTTGG
CTATACGAAA GAAAACATAA GGAAAAGACT CTTTAGTTTG AAAAAGTGGA CACCGGAACC
2821 CCACCAAAAG CTAACAAATA AAGGCATATG AAGTAGCCAA GGCCTTTTCT AGTTATATCT
GGTGGTTTTC GATTGTTTAT TTCCGTATAC TTCATCGGTT CCGGAAAAGA TCAATATAGA
2881 ATAACACTGA GTTCATTTCA TCATTTATTT TCCTGACTTC CTCCTGGGTC CATATGAGCA
TATTGTGACT CAAGTAAAGT AGTAAATAAA AGGACTGAAG GAGGACCCAG GTATACTCGT
2941 GTCTTAGAAT GAATATTAGC TGAATAATCC AAATACATAG TAGATGTTGA TTTGGGTTTT
CAGAATCTTA CTTATAATCG ACTTATTAGG TTTATGTATC ATCTACAACT AAACCCAAAA
3001 CTAAGCAATC CAAGACTTGT ATGACAGTAA GATGTATTAC CATCCAACAC ACATCTCAGC
GATTCGTTAG GTTCTGAACA TACTGTCATT CTACATAATG GTAGGTTGTG TGTAGAGTCG
3061 ATGATATAAA TGCAAGGTAT ATTGTGAAGA AAAATTTTTA ATTATGTCAA AGTGCTTACT
TACTATATTT ACGTTCCATA TAACACTTCT TTTTAAAAAT TAATACAGTT TCACGAATGA
3121 TTAGAAGGTC ATCTATCTGT CCCAAAGCTG TGAATATATA TATTGAAGGT AATGAATAGA
AATCTTCCAG TAGATAGACA GGGTTTCGAC ACTTATATAT ATAACTTCCA TTACTTATCT
3181 TGAAGCTAAC CTTGTAAAAA TGAGTAGTGT GAAATACAAC TACAATTATG AACATCTGTC
ACTTCGATTG GAACATTTTT ACTCATCACA CTTTATGTTG ATGTTAATAC TTGTAGACAG
3241 ACTAAAGAGG CAAAGAAACT TGAAGATTGC TTTTGCAAAT GGGCTCCTAT TAATAAAAAG
TGATTTCTCC GTTTCTTTGA ACTTCTAACG AAAACGTTTA CCCGAGGATA ATTATTTTTC
3301 TACTTTTGAG GTCTGGCTCA GACTCTATTG TAGTACTTAG GGTAAGACCC TCCTCCTGTA
ATGAAAACTC CAGACCGAGT CTGAGATAAC ATCATGAATC CCATTCTGGG AGGAGGACAT
3361 TGGGCTTTCA TTTTCTTTCT TGCTTCCCTC ATTTGCCCTT CCATGAATAC TAGCTGATAA
ACCCGAAAGT AAAAGAAAGA ACGAAGGGAG TAAACGGGAA GGTACTTATG ATCGACTATT
3421 ACATTGACTA TAAAAGATAT GAGGCCAAAC TTGAGCTGTC CCATTTTAAT AAATCTGTAT
TGTAACTGAT ATTTTCTATA CTCCGGTTTG AACTCGACAG GGTAAAATTA TTTAGACATA
3481 AAATAATATT TGTTCTACAA AAGTATTATC TAAATAAATG TTACTTTCTG TCTTAAAATC
TTTATTATAA ACAAGATGTT TTCATAATAG ATTTATTTAC AATGAAAGAC AGAATTTTAG
3541 CCTCAACAAA TCCCCACTAT CTAGAGAATA AGATTGACAT TCCCTGGAAT CACAGCATGC
GGAGTTGTTT AGGGGTGATA GATCTCTTAT TCTAACTGTA AGGGACCTTA GTGTCGTACG
3601 TTTGTCTGCC ATTATCTGAC CCCTTTCTCT TTCTCTCTTC TCACCTCCAT CTACTCCTTT
AAACAGACGG TAATAGACTG GGGAAAGAGA AAGAGAGAAG AGTGGAGGTA GATGAGGAAA
3661 TTCCTTGCAA TTCATGACCC AGATTCACTG TTTGATTTGG CTTGCATGTG TGTGTGCTGA
AAGGAACGTT AAGTACTGGG TCTAAGTGAC AAACTAAACC GAACGTACAC ACACACGACT
3721 GTTGCGTCTG ACTGTTATCA ACCCCATGAA TGATAGTCCA CCAGGCTCTA CTGTCCATGA
CAACGCAGAC TGACAATAGT TGGGGTACTT ACTATCAGGT GGTCCGAGAT GACAGGTACT
3781 AATTTTCCAG TCAAGAATAC TGGAGTGGAT TGCATTTCCT ACTCCATTTG ATTAATTTAG
TTAAAAGGTC AGTTCTTATG ACCTCACCTA ACGTAAAGGA TGAGGTAAAC TAATTAAATC
3841 TGACTTTTAA ATTTCTTTTT CCATATTCGG GAGCCTATTC TTCCTTTTTA GTCTATACTC
ACTGAAAATT TAAAGAAAAA GGTATAAGCC CTCGGATAAG AAGGAAAAAT CAGATATGAG
3901 TCTTCACTCT TCAGGTCTAA GGTATCATCG TGTGCTTGTT AGCTTGTTAC TTTCTCCATT
AGAAGTGAGA AGTCCAGATT CCATAGTAGC ACACGAACAA TCGAACAATG AAAGAGGTAA
3961 ATAGCTTAAG CACTAACAAC TGTTCAGGTT GGCATGAAAT TGTGTTCTTT GTGTGGCCTG
TATCGAATTC GTGATTGTTG ACAAGTCCAA CCGTACTTTA ACACAAGAAA CACACCGGAC
4021 TATATTTCTG TTGTGTATTA GAATTTACCC CAAGATCTCA AAGACCCACT GAATACTAAA
ATATAAAGAC AACACATAAT CTTAAATGGG GTTCTAGAGT TTCTGGGTGA CTTATGATTT
4081 GAGACCTCAT TGTGGTTACA ATAATTTGGG GACTGGGCCA AAACTACCGT GCATCCCAGC
CTCTGGAGTA ACACCAATGT TATTAAACCC CTGACCCGGT TTTGATGGCA CGTAGGGTCG
4141 CAAGATCTGT AGCTACTGGA CAATTTCATT TCCTTTATCA GATTGTGAGT TATTCCTGTT
GTTCTAGACA TCGATGACCT GTTAAAGTAA AGGAAATAGT CTAACACTCA ATAAGGACAA
4201 AAAATGCTCC CCAGAATTTC TGGGGACAGA AAAATAGGAA GAATTCATTT CCTAATCATG
TTTTACGAGG GGTCTTAAAG ACCCCTGTCT TTTTATCCTT CTTAAGTAAA GGATTAGTAC
4261 CAGATTTCTA GGAATTCAAA TCCACTGTTG GTTTTATTTC AAACCACAAA ATTAGCATGC
GTCTAAAGAT CCTTAAGTTT AGGTGACAAC CAAAATAAAG TTTGGTGTTT TAATCGTACG
4321 CATTAAATAC TATATATAAA CAGCCACTAA ATCAGATCAT TATCCATTCA GCTTCTCCTT
GTAATTTATG ATATATATTT GTCGGTGATT TAGTCTAGTA ATAGGTAAGT CGAAGAGGAA
4381 CACTTCTTCT CCTCTACTTT GGAAAAAAGG TAAGAATCTC AGATATAATT TCAGGTGTAT
GTGAAGAAGA GGAGATGAAA CCTTTTTTCC ATTCTTAGAG TCTATATTAA AGTCCACATA
4441 CTGCTACTCA TCTTTATTTT GGACTAGGTT AAAATGTAGA AAGAACATAA TTGCTTAAAA
GACGATGAGT AGAAATAAAA CCTGATCCAA TTTTACATCT TTCTTGTATT AACGAATTTT
4501 TAGATCTTAA AAATAAGGGT GTTTAAGATA AGGTTTACAC TATTTTCAGC AGATATGTTA
ATCTAGAATT TTTATTCCCA CAAATTCTAT TCCAAATGTG ATAAAAGTCG TCTATACAAT
4561 AAAAATAGAA GTGACTATAA AGACTTGATA AAAATTATAG TGACTGCAAA TGTTTTAGGA
TTTTTATCTT CACTGATATT TCTGAACTAT TTTTAATATC ACTGACGTTT ACAAAATCCT
4621 ATATAATAAG ATATAATAAC GGTGGTTGCT ATTTTCTTTA GCACAAGACT AGTTAACAGG
TATATTATTC TATATTATTG CCACCAACGA TAAAAGAAAT CGTGTTCTGA TCAATTGTCC
4681 CTGTATTAAA AGATCTTTTC TTGAATTAAA TATTTTCAAT TTGATTAAAC CTACCTCAGC
GACATAATTT TCTAGAAAAG AACTTAATTT ATAAAAGTTA AACTAATTTG GATGGAGTCG
4741 CATAAAGGCA AGCACATTTC ATTTATACTA TGGGGATTTG AATAATTATT ACTGAAGAAG
GTATTTCCGT TCGTGTAAAG TAAATATGAT ACCCCTAAAC TTATTAATAA TGACTTCTTC
4801 CTCTACCAAC AAAAAGTTTA TAGAGCTATC ATATTTAGTC AAGAGATAAA GAGGGTTGTT
GAGATGGTTG TTTTTCAAAT ATCTCGATAG TATAAATCAG TTCTCTATTT CTCCCAACAA
4861 AGGATATATA TGCTATTTGA AAGGTATTTA TAAAAGAAGA GTATATTTAT CAAAATTTCT
TCCTATATAT ACGATAAACT TTCCATAAAT ATTTTCTTCT CATATAAATA GTTTTAAAGA
4921 CAGAACATCC AAATTTCAAG TTTATCATTT ATCTTACAAT ATTTCAAAAA TATTAAAATA
GTCTTGTAGG TTTAAAGTTC AAATAGTAAA TAGAATGTTA TAAAGTTTTT ATAATTTTAT
4981 GATACTGAAA TACAGAAGTA AATTAAAGAG AAAGTATTTT ACTTGGTAAA AAAATTCTAG
CTATGACTTT ATGTCTTCAT TTAATTTCTC TTTCATAAAA TGAACCATTT TTTTAAGATC
5041 GTTGGACAGA GAGTGCCAGG AAACAAAAAC AATGAAAAAT GTGACCTGAC AGGAATTATA
CAACCTGTCT CTCACGGTCC TTTGTTTTTG TTACTTTTTA CACTGGACTG TCCTTAATAT
5101 GCTCAAAGTA TAGTAGTAAG TAATGAAATG GCTTAAAAAT TGGTATATAA AATGCTAGTT
CGAGTTTCAT ATCATCATTC ATTACTTTAC CGAATTTTTA ACCATATATT TTACGATCAA
5161 ATAAAATAAA CAAAATGCAA TAATATCCTC CCTACATGTA ATGAATTCTA GGTATTATGC
TATTTTATTT GTTTTACGTT ATTATAGGAG GGATGTACAT TACTTAAGAT CCATAATACG
5221 TCTTTTTGGA AGTCTTGACA ATAAAAATTT TTTTAGAAGT TTATAGGCAT CTTGAATAAA
AGAAAAACCT TCAGAACTGT TATTTTTAAA AAAATCTTCA AATATCCGTA GAACTTATTT
5281 GTGAAACAAA TTAAGAATTA GTATCCATGA GAAAAATATA GAACAATTTT CCTAATTTAG
CACTTTGTTT AATTCTTAAT CATAGGTACT CTTTTTATAT CTTGTTAAAA GGATTAAATC
5341 TTTGAAAATC TGGGATTGAA GATGTGTGTC AAGAGATGTT GGTGGCAAGA ACATTTTTTT
AAACTTTTAG ACCCTAACTT CTACACACAG TTCTCTACAA CCACCGTTCT TGTAAAAAAA
5401 TTCAAGAACT TATAAAAATG CAACAAAACA AACCATTTAA TACATTTTGG TCAAAATCAA
AAGTTCTTGA ATATTTTTAC GTTGTTTTGT TTGGTAAATT ATGTAAAACC AGTTTTAGTT
5461 TAATGTATTT TATTTTATGC TCCAAGGAGC ATAAAATTGG GGACTGGGCA AGAGAAACTG
ATTACATAAA ATAAAATACG AGGTTCCTCG TATTTTAACC CCTGACCCGT TCTCTTTGAC
5521 ACACCCTGGT AAATTACCAA GAGATAAGTA CACAGTTCTA TGTAGAGAAA ATAAGCATAG
TGTGGGACCA TTTAATGGTT CTCTATTCAT GTGTCAAGAT ACATCTCTTT TATTCGTATC
5581 TGTATGATCT CTAAAATTAT GTGAGACAAA GGAGAGATGA CATTAGGCAT GTGGGGATGA
ACATACTAGA GATTTTAATA CACTCTGTTT CCTCTCTACT GTAATCCGTA CACCCCTACT
5641 AGACTGAGTA GAGAAGAAAC AATCTAATCA GTCCAAGAAA ACATCTCGAT CAGTGGAACA
TCTGACTCAT CTCTTCTTTG TTAGATTAGT CAGGTTCTTT TGTAGAGCTA GTCACCTTGT
5701 AATAGAAGAA ATGCTAAAAT GAAACAGAAG TCTTACTGGA AATAAAAGAT ATGCATAAGA
TTATCTTCTT TACGATTTTA CTTTGTCTTC AGAATGACCT TTATTTTCTA TACGTATTCT
5761 CAAAAATTCA TGAAAATCAC TTAGTTTAGC AGAGAAAAGA TAAAAATAAA GTATGACCTT
GTTTTTAAGT ACTTTTAGTG AATCAAATCG TCTCTTTTCT ATTTTTATTT CATACTGGAA
5821 CTTCATATAC ATTGTTTGAT CATATGCACC TCAATAAAAC TGAGTCTCCA ACAGAAATGA
GAAGTATATG TAACAAACTA GTATACGTGG AGTTATTTTG ACTCAGAGGT TGTCTTTACT
5881 AACATTAATA TTTTGTTCAC TGCTCTAATC CCAGAATCTA AGCGATATCT GGCAATAAAA
TTGTAATTAT AAAACAAGTG ACGAGATTAG GGTCTTAGAT TCGCTATAGA CCGTTATTTT
5941 ATAATAAATA TATATTTTTT AATAAATGAA TCAACCACTT AATTTTTCTG TAAATATCTG
TATTATTTAT ATATAAAAAA TTATTTACTT AGTTGGTGAA TTAAAAAGAC ATTTATAGAC
6001 TAACTTCTCT TCTGTCTTTC CAAAAACACT CATAAGTACT GTGAATGAGA TGAAAAAGAG
ATTGAAGAGA AGACAGAAAG GTTTTTGTGA GTATTCATGA CACTTACTCT ACTTTTTCTC
6061 TGAAGTAGGA TATAGGCTGT TAGCAGAAAA CATCTGAATG GCTGGCAGTG AAACATTAAC
ACTTCATCCT ATATCCGACA ATCGTCTTTT GTAGACTTAC CGACCGTCAC TTTGTAATTG
6121 TTGAAATGTA AGATTAATGA GTAATAGTAA ATTTTAACCT TGGCCATATG ATAAAATGTT
AACTTTACAT TCTAATTACT CATTATCATT TAAAATTGGA ACCGGTATAC TATTTTACAA
6181 CATTAATATT TTTCTAGAAT ACAGGGCTTT TTGTTTTTGC CATGAGGTTT GCAGGATCTT
GTAATTATAA AAAGATCTTA TGTCCCGAAA AACAAAAACG GTACTCCAAA CGTCCTAGAA
6241 GGTTCCCTGA CCAGGGATCA AACCTGCACT CCCCTGGAAG CATGGAGTCT TGGACATTTG
CCAAGGGACT GGTCCCTAGT TTGGACGTGA GGGGACCTTC GTACCTCAGA ACCTGTAAAC
6301 TATTATACAC TATCTTTGGT TCCTTTTAAA GGGAAGTAAT TTTACTTAAA TAAGAAAATA
ATAATATGTG ATAGAAACCA AGGAAAATTT CCCTTCATTA AAATGAATTT ATTCTTTTAT
6361 GATTGACAAG TAATACGCTG TTTCCTCATC TTCCCATTCA CAGGAATCGA GAGCCATGAA
CTAACTGTTC ATTATGCGAC AAAGGAGTAG AAGGGTAAGT GTCCTTAGCT CTCGGTACTT
6421 GGTCCTCATC CTTGCCTGTC TGGTGGCTCT GGCCATTGCG ATCGC
CCAGGAGTAG GAACGGACAG ACCACCGAGA CCGGTAACGC TAGCG
The merchant sells plasmid vector and the Central Shanxi Plain milk goat beta-casein gene that is inserted and starts regional sequence 6465bp to constitute total length jointly be that the Central Shanxi Plain milk goat beta-casein gene opener expression vector sequence of 11838bp is as follows:
1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
CTGCCTAGCC CTCTAGAGGG CTAGGGGATA CCACGTGAGA GTCATGTTAG ACGAGACTAC
61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
GGCGTATCAA TTCGGTCATA GACGAGGGAC GAACACACAA CCTCCAGCGA CTCATCACGC
121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
GCTCGTTTTA AATTCGATGT TGTTCCGTTC CGAACTGGCT GTTAACGTAC TTCTTAGACG
181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT
AATCCCAATC CGCAAAACGC GACGAAGCGC TACATGCCCG GTCTATATGC GCAACTGTAA
241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA
CTAATAACTG ATCAATAATT ATCATTAGTT AATGCCCCAG TAATCAAGTA TCGGGTATAT
301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
ACCTCAAGGC GCAATGTATT GAATGCCATT TACCGGGCGG ACCGACTGGC GGGTTGCTGG
361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
GGGCGGGTAA CTGCAGTTAT TACTGCATAC AAGGGTATCA TTGCGGTTAT CCCTGAAAGG
421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
TAACTGCAGT TACCCACCTC ATAAATGCCA TTTGACGGGT GAACCGTCAT GTAGTTCACA
481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
TAGTATACGG TTCATGCGGG GGATAACTGC AGTTACTGCC ATTTACCGGG CGGACCGTAA
541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
TACGGGTCAT GTACTGGAAT ACCCTGAAAG GATGAACCGT CATGTAGATG CATAATCAGT
601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
AGCGATAATG GTACCACTAC GCCAAAACCG TCATGTAGTT ACCCGCACCT ATCGCCAAAC
661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
TGAGTGCCCC TAAAGGTTCA GAGGTGGGGT AACTGCAGTT ACCCTCAAAC AAAACCGTGG
721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
TTTTAGTTGC CCTGAAAGGT TTTACAGCAT TGTTGAGGCG GGGTAACTGC GTTTACCCGC
781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA
CATCCGCACA TGCCACCCTC CAGATATATT CGTCTCGAGA GACCGATTGA TCTCTTGGGT
841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC
GACGAATGAC CGAATAGCTT TAATTATGCT GAGTGATATC CCTCTGGGTT CGACCGATCG
841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC
GACGAATGAC CGAATAGCTT TAATTATGCT GAGTGATATC CCTCTGGGTT CGACCGATCG
901 GTTTAAACGG GCCCTCTAGA CAGATGATTT TGCAACCCCC TGCCTCAGGA GACACTGGGA
CAAATTTGCC CGGGAGATCT GTCTACTAAA ACGTTGGGGG ACGGAGTCCT CTGTGACCCT
961 AATTTCCTGA GACATTTTTG ATTCCAAAAG CTGTGCAGTT GGTGCTTCTA CCATCTTCGT
TTAAAGGACT CTGTAAAAAC TAAGGTTTTC GACACGTCAA CCACGAAGAT GGTAGAAGCA
1021 GGTAGAGGTC AAGGATGCTG CTAAACATTC TACAACACAT TAAGAAAACC CCCACAACAA
CCATCTCCAG TTCCTACGAC GATTTGTAAG ATGTTGTGTA ATTCTTTTGG GGGTGTTGTT
1081 AGAATTCTTC CGCCAAAAAT ATCAATAATA TGAAGGTTGA AAAATACTGG TCTAGCATGT
TCTTAAGAAG GCGGTTTTTA TAGTTATTAT ACTTCCAACT TTTTATGACC AGATCGTACA
1141 AGTATGTGCT CAATAGCAAG GAGAGAAAAG AAAGCCTTCC TCACTGATTA ATGCAAAGAA
TCATACACGA GTTATCGTTC CTCTCTTTTC TTTCGGAAGG AGTGACTAAT TACGTTTCTT
1201 ATAGAGGAAA ACAATAGAAT GGGAAAGACT AGAGAGCTCT TCAAGCAAAT TAGAGATATC
TATCTCCTTT TGTTATCTTA CCCTTTCTGA TCTCTCGAGA AGTTCGTTTA ATCTCTATAG
1261 AAGGGAACAT TTCACGCAAA GATGGGCACA ATAAAGGACA GAAATTTTAT GGAGGAGTTG
TTCCCTTGTA AAGTGCGTTT CTACCCGTGT TATTTCCTGT CTTTAAAATA CCTCCTCAAC
1321 CTGATGGAGA GGGAGGCCTG GCGTGCTGCG ATTCCTGGGG TCGCAAAGAG TCGGACACAA
GACTACCTCT CCCTCCGGAC CGCACGACGC TAAGGACCCC AGCGTTTCTC AGCCTGTGTT
1381 CTGAGCGACT GAATTGAACT GAACTGAACT GGACAAAGCA GAAGATATTA AGAAGAGGTG
GACTCGCTGA CTTAACTTGA CTTGACTTGA CCTGTTTCGT CTTCTATAAT TCTTCTCCAC
1441 GTAAGAATAC ACAGAAGAAC AATATAAAAA AGATCTTCAT GACCCAGATA ACCACGATGA
CATTCTTATG TGTCTTCTTG TTATATTTTT TCTAGAAGTA CTGGGTCTAT TGGTGCTACT
1501 TGTGATCACT CACCTAGAGC CAGACACCCT GGAATGCAAA GTCAAACGGC CTTAGAAAGC
ACACTAGTGA GTGGATCTCG GTCTGTGGGA CCTTACGTTT CAGTTTGCCG GAATCTTTCG
1561 CTCACTATGA ACAAAGCTAG TGGAGGTAAT GGAATTCCAG TTGAGCTATT TCAAATCTTA
GAGTGATACT TGTTTCGATC ACCTCCATTA CCTTAAGGTC AACTCGATAA AGTTTAGAAT
1621 AAAGGTGATG CTGTGAAAGT GCTGCACTCA ATATGTCAGC AAATTTGGAA AACTCAGCAG
TTTCCACTAC GACACTTTCA CGACGTGAGT TATACAGTCG TTTAAACCTT TTGAGTCGTC
1681 TGGCCACAGG ACTGCCACAA TCCCAAAGAA AAGCAATGAC AAAGAATGTT CAAACACCCA
ACCGGTGTCC TGACGGTGTT AGGGTTTCTT TTCGTTACTG TTTCTTACAA GTTTGTGGGT
1741 CATGATTGCA CTCATCTCAC ATGCTAGCAA AATAACTCTC AAAATTCTCC AAGCCAGGCT
GTACTAACGT GAGTAGAGTG TACGATCGTT TTATTGAGAG TTTTAAGAGG TTCGGTCCGA
1801 CCAACAGTAC GTGGACCATG AACTTCCAGA TGTTCAAGCT GGATTTAGAA AAGGCAGAGG
GGTTGTCATG CACCTGGTAC TTGAAGGTCT ACAAGTTCGA CCTAAATCTT TTCCGTCTCC
1861 AACCAGAGAT CAAATTGCCA ACATCCATTG GATCATCAAA AAAGCACGAG AGTTCCAGAA
TTGGTCTCTA GTTTAACGGT TGTAGGTAAC CTAGTAGTTT TTTCGTGCTC TCAAGGTCTT
1921 AAACATCTGC TTTATTGACT ACGCTAAAGC CTTTGATTGT GTGGATCACA ATAAACTGTG
TTTGTAGACG AAATAACTGA TGCGATTTCG GAAACTAACA CACCTAGTGT TATTTGACAC
1981 GAAAATTCTT CAAGAGATGG GAATACCAGA CCACTTTACC TGCCTCCTGA GAAATCTGTA
CTTTTAAGAA GTTCTCTACC CTTATGGTCT GGTGAAATGG ACGGAGGACT CTTTAGACAT
2041 TACAGGTCCA GAAGCAGCAG TTAGAACTGG ACATGGAACA ACAGACTGGT TCCAAACTGC
ATGTCCAGGT CTTCGTCGTC AATCTTGACC TGTACCTTGT TGTCTGACCA AGGTTTGACG
2101 GAAAGGGGTA CATCAAGGAA TATTCATTGG AAGGATTGAT GCTGAAGCTG AAACTCCTAT
CTTTCCCCAT GTAGTTCCTT ATAAGTAACC TTCCTAACTA CGACTTCGAC TTTGAGGATA
2161 ACTTTGGCCA CCTAATGTGA AGATCTGACT CATTGGAAAA GACTCCAATG CTGGGAAAGA
TGAAACCGGT GGATTACACT TCTAGACTGA GTAACCTTTT CTGAGGTTAC GACCCTTTCT
2221 TTGAAGGCAG GAGAAGAGGA TGACAGAGGA TGAGATGGTT GGATGGGATC ACTGACTCAA
AACTTCCGTC CTCTTCTCCT ACTGTCTCCT ACTCTACCAA CCTACCCTAG TGACTGAGTT
2281 TGGACATGAG TTTGAGTAAG CTCCAGGGGT TGGTGGTGGA CAGGAAAGCC TGGCGTGCTG
ACCTGTACTC AAACTCATTC GAGGTCCCCA ACCACCACCT GTCCTTTCGG ACCGCACGAC
2341 CAGTCCACAA GGTCACAAAG ATTCGGACAT GACTGAGTGA CTGAACTGAT ACTGATGTGC
GTCAGGTGTT CCAGTGTTTC TAAGCCTGTA CTGACTCACT GACTTGACTA TGACTACACG
2401 TCAACAAATG TATCTTGAAC TTGTGTGAAG TTCTATGGTC ACATGTAAAG GAAGAATAAT
AGTTGTTTAC ATAGAACTTG AACACACTTC AAGATACCAG TGTACATTTC CTTCTTATTA
2461 CAGGATTAGC TGTGTGTCTT AGGAATCAGG GTTCTGAGTT TTATGTGTTC ATAGTATCTG
GTCCTAATCG ACACACAGAA TCCTTAGTCC CAAGACTCAA AATACACAAG TATCATAGAC
2521 CTGGTTCACA AAACATTTTT CTTATTCTCT GGTTCTTGAT TTACTTTATA AAGTAATCTT
GACCAAGTGT TTTGTAAAAA GAATAAGAGA CCAAGAACTA AATGAAATAT TTCATTAGAA
2581 AATAGTTATA CTTCACATAG ATACGAAATT ATTATATTTG GATAATCTCA TGGAAAGGAT
TTATCAATAT GAAGTGTATC TATGCTTTAA TAATATAAAC CTATTAGAGT ACCTTTCCTA
2641 TAAATACTCC ATCTATTACG AGTAATGCTG AACTATCTAC TCCTACCTAA TAATTTGTCA
ATTTATGAGG TAGATAATGC TCATTACGAC TTGATAGATG AGGATGGATT ATTAAACAGT
2701 GAATTCACTA ATTCTGTGTT ATATTGTTTC TAAATCTGAA TCATTATATG AATCCTCAGT
CTTAAGTGAT TAAGACACAA TATAACAAAG ATTTAGACTT AGTAATATAC TTAGGAGTCA
2761 ATTTTGTTTT CCTTCCTCTA TATTTTGGAA TTTATTAAAC AGTGCTTCAA ATAATTTTTA
TAAAACAAAA GGAAGGAGAT ATAAAACCTT AAATAATTTG TCACGAAGTT TATTAAAAAT
2821 GGAAACTGAA GTTTTTAGTA ACAGCTCTAT CTCTAAATAG CTTTAGTATC TTGAAAAAGT
CCTTTGACTT CAAAAATCAT TGTCGAGATA GAGATTTATC GAAATCATAG AACTTTTTCA
2881 AATACAAATT CTCACATCCT TAATTTCCTC TTCTCTAAAA TATCTTTAAA ATATTCTATG
TTATGTTTAA GAGTGTAGGA ATTAAAGGAG AAGAGATTTT ATAGAAATTT TATAAGATAC
2941 AATGATATCT CTTAATATTT ATTTTTTTGG CAATCCAACA CAGCTTATGG GATCTTAGTT
TTACTATAGA GAATTATAAA TAAAAAAACC GTTAGGTTGT GTCGAATACC CTAGAATCAA
3001 CCCCAGTGAG GGATTATATC CATGCCAACT GCAGTGAAAG TACAAAATCC TAAACTGGAC
GGGGTCACTC CCTAATATAG GTACGGTTGA CGTCACTTTC ATGTTTTAGG ATTTGACCTG
3061 TCACCAGGGA TTTCCCAATA TCTCCTCTAG TTCTTATTTC TGAATATTTT TGGTCCCTTT
AGTGGTCCCT AAAGGGTTAT AGAGGAGATC AAGAATAAAG ACTTATAAAA ACCAGGGAAA
3121 ATTGTACTCT TCATCCAACT TTTCTATTGA TTTCTTTCTT GAGGTTATTA TTTACTTGGT
TAACATGAGA AGTAGGTTGA AAAGATAACT AAAGAAAGAA CTCCAATAAT AAATGAACCA
3181 TTCAGTTAGA AATATATGCA AATCTCAGGA CTGCATATTT CAGATTCATT GGCCAATATG
AAGTCAATCT TTATATACGT TTAGAGTCCT GACGTATAAA GTCTAAGTAA CCGGTTATAC
3241 GGAAAAAACC TTTGGCTGAA CAAATCATGC TTATAAAAAA TAGTACTAGA GCATCCTACT
CCTTTTTTGG AAACCGACTT GTTTAGTACG AATATTTTTT ATCATGATCT CGTAGGATGA
3301 TTGACTATAT CTTGCTCCTC ATTCAGGGTT ATCTAATACA ATTTCCCCAC ATGAAATTCT
AACTGATATA GAACGAGGAG TAAGTCCCAA TAGATTATGT TAAAGGGGTG TACTTTAAGA
3361 TTTGCATTAT AAAAATGGAA GCTCTTAGGT AACATTGCAA AAATTCGAGT TGCTCATATG
AAACGTAATA TTTTTACCTT CGAGAATCCA TTGTAACGTT TTTAAGCTCA ACGAGTATAC
3421 GCACTTTGCT TCTTACTGGT CATTGTGTTC TGAGGCTTAC CTGGACAGGT GGTACCTGAT
CGTGAAACGA AGAATGACCA GTAACACAAG ACTCCGAATG GACCTGTCCA CCATGGACTA
3481 GTCATCTTAA ATTGCTGGCT TTTTGATTTT CCATTGGACA AGCTTCTTTC TTTAGTATAT
CAGTAGAATT TAACGACCGA AAAACTAAAA GGTAACCTGT TCGAAGAAAG AAATCATATA
3541 TGTTAAGGAT TTCCTTGATC AAGATTTTAC CTACTTTTCT GGTCCAATTG GTGAGAGACA
ACAATTCCTA AAGGAACTAG TTCTAAAATG GATGAAAAGA CCAGGTTAAC CACTCTCTGT
3601 GTCATAAGGA AATGCTGTGT TTATTGCACA ATATGTAAAG CATCTTCCTG AGAAAATAAA
CAGTATTCCT TTACGACACA AATAACGTGT TATACATTTC GTAGAAGGAC TCTTTTATTT
3661 AGGGAAATGT TGAATGGGAA GGATATGCTT TCTTTTGTAT TCCTTTTCTG AGAAATCAAA
TCCCTTTACA ACTTACCCTT CCTATACGAA AGAAAACATA AGGAAAAGAC TCTTTAGTTT
3721 CTTTTTCACC TGTGGCCTTG GCCACCAAAA GCTAACAAAT AAAGGCATAT GAAGTAGCCA
GAAAAAGTGG ACACCGGAAC CGGTGGTTTT CGATTGTTTA TTTCCGTATA CTTCATCGGT
3781 AGGCCTTTTC TAGTTATATC TATAACACTG AGTTCATTTC ATCATTTATT TTCCTGACTT
TCCGGAAAAG ATCAATATAG ATATTGTGAC TCAAGTAAAG TAGTAAATAA AAGGACTGAA
3841 CCTCCTGGGT CCATATGAGC AGTCTTAGAA TGAATATTAG CTGAATAATC CAAATACATA
GGAGGACCCA GGTATACTCG TCAGAATCTT ACTTATAATC GACTTATTAG GTTTATGTAT
3901 GTAGATGTTG ATTTGGGTTT TCTAAGCAAT CCAAGACTTG TATGACAGTA AGATGTATTA
CATCTACAAC TAAACCCAAA AGATTCGTTA GGTTCTGAAC ATACTGTCAT TCTACATAAT
3961 CCATCCAACA CACATCTCAG CATGATATAA ATGCAAGGTA TATTGTGAAG AAAAATTTTT
GGTAGGTTGT GTGTAGAGTC GTACTATATT TACGTTCCAT ATAACACTTC TTTTTAAAAA
4021 AATTATGTCA AAGTGCTTAC TTTAGAAGGT CATCTATCTG TCCCAAAGCT GTGAATATAT
TTAATACAGT TTCACGAATG AAATCTTCCA GTAGATAGAC AGGGTTTCGA CACTTATATA
4081 ATATTGAAGG TAATGAATAG ATGAAGCTAA CCTTGTAAAA ATGAGTAGTG TGAAATACAA
TATAACTTCC ATTACTTATC TACTTCGATT GGAACATTTT TACTCATCAC ACTTTATGTT
4141 CTACAATTAT GAACATCTGT CACTAAAGAG GCAAAGAAAC TTGAAGATTG CTTTTGCAAA
GATGTTAATA CTTGTAGACA GTGATTTCTC CGTTTCTTTG AACTTCTAAC GAAAACGTTT
4201 TGGGCTCCTA TTAATAAAAA GTACTTTTGA GGTCTGGCTC AGACTCTATT GTAGTACTTA
ACCCGAGGAT AATTATTTTT CATGAAAACT CCAGACCGAG TCTGAGATAA CATCATGAAT
4261 GGGTAAGACC CTCCTCCTGT ATGGGCTTTC ATTTTCTTTC TTGCTTCCCT CATTTGCCCT
CCCATTCTGG GAGGAGGACA TACCCGAAAG TAAAAGAAAG AACGAAGGGA GTAAACGGGA
4321 TCCATGAATA CTAGCTGATA AACATTGACT ATAAAAGATA TGAGGCCAAA CTTGAGCTGT
AGGTACTTAT GATCGACTAT TTGTAACTGA TATTTTCTAT ACTCCGGTTT GAACTCGACA
4381 CCCATTTTAA TAAATCTGTA TAAATAATAT TTGTTCTACA AAAGTATTAT CTAAATAAAT
GGGTAAAATT ATTTAGACAT ATTTATTATA AACAAGATGT TTTCATAATA GATTTATTTA
4441 GTTACTTTCT GTCTTAAAAT CCCTCAACAA ATCCCCACTA TCTAGAGAAT AAGATTGACA
CAATGAAAGA CAGAATTTTA GGGAGTTGTT TAGGGGTGAT AGATCTCTTA TTCTAACTGT
4501 TTCCCTGGAA TCACAGCATG CTTTGTCTGC CATTATCTGA CCCCTTTCTC TTTCTCTCTT
AAGGGACCTT AGTGTCGTAC GAAACAGACG GTAATAGACT GGGGAAAGAG AAAGAGAGAA
4561 CTCACCTCCA TCTACTCCTT TTTCCTTGCA ATTCATGACC CAGATTCACT GTTTGATTTG
GAGTGGAGGT AGATGAGGAA AAAGGAACGT TAAGTACTGG GTCTAAGTGA CAAACTAAAC
4621 GCTTGCATGT GTGTGTGCTG AGTTGCGTCT GACTGTTATC AACCCCATGA ATGATAGTCC
CGAACGTACA CACACACGAC TCAACGCAGA CTGACAATAG TTGGGGTACT TACTATCAGG
4681 ACCAGGCTCT ACTGTCCATG AAATTTTCCA GTCAAGAATA CTGGAGTGGA TTGCATTTCC
TGGTCCGAGA TGACAGGTAC TTTAAAAGGT CAGTTCTTAT GACCTCACCT AACGTAAAGG
4741 TACTCCATTT GATTAATTTA GTGACTTTTA AATTTCTTTT TCCATATTCG GGAGCCTATT
ATGAGGTAAA CTAATTAAAT CACTGAAAAT TTAAAGAAAA AGGTATAAGC CCTCGGATAA
4801 CTTCCTTTTT AGTCTATACT CTCTTCACTC TTCAGGTCTA AGGTATCATC GTGTGCTTGT
GAAGGAAAAA TCAGATATGA GAGAAGTGAG AAGTCCAGAT TCCATAGTAG CACACGAACA
4861 TAGCTTGTTA CTTTCTCCAT TATAGCTTAA GCACTAACAA CTGTTCAGGT TGGCATGAAA
ATCGAACAAT GAAAGAGGTA ATATCGAATT CGTGATTGTT GACAAGTCCA ACCGTACTTT
4921 TTGTGTTCTT TGTGTGGCCT GTATATTTCT GTTGTGTATT AGAATTTACC CCAAGATCTC
AACACAAGAA ACACACCGGA CATATAAAGA CAACACATAA TCTTAAATGG GGTTCTAGAG
4981 AAAGACCCAC TGAATACTAA AGAGACCTCA TTGTGGTTAC AATAATTTGG GGACTGGGCC
TTTCTGGGTG ACTTATGATT TCTCTGGAGT AACACCAATG TTATTAAACC CCTGACCCGG
5041 AAAACTACCG TGCATCCCAG CCAAGATCTG TAGCTACTGG ACAATTTCAT TTCCTTTATC
TTTTGATGGC ACGTAGGGTC GGTTCTAGAC ATCGATGACC TGTTAAAGTA AAGGAAATAG
5101 AGATTGTGAG TTATTCCTGT TAAAATGCTC CCCAGAATTT CTGGGGACAG AAAAATAGGA
TCTAACACTC AATAAGGACA ATTTTACGAG GGGTCTTAAA GACCCCTGTC TTTTTATCCT
5161 AGAATTCATT TCCTAATCAT GCAGATTTCT AGGAATTCAA ATCCACTGTT GGTTTTATTT
TCTTAAGTAA AGGATTAGTA CGTCTAAAGA TCCTTAAGTT TAGGTGACAA CCAAAATAAA
5221 CAAACCACAA AATTAGCATG CCATTAAATA CTATATATAA ACAGCCACTA AATCAGATCA
GTTTGGTGTT TTAATCGTAC GGTAATTTAT GATATATATT TGTCGGTGAT TTAGTCTAGT
5281 TTATCCATTC AGCTTCTCCT TCACTTCTTC TCCTCTACTT TGGAAAAAAG GTAAGAATCT
AATAGGTAAG TCGAAGAGGA AGTGAAGAAG AGGAGATGAA ACCTTTTTTC CATTCTTAGA
5341 CAGATATAAT TTCAGGTGTA TCTGCTACTC ATCTTTATTT TGGACTAGGT TAAAATGTAG
GTCTATATTA AAGTCCACAT AGACGATGAG TAGAAATAAA ACCTGATCCA ATTTTACATC
5401 AAAGAACATA ATTGCTTAAA ATAGATCTTA AAAATAAGGG TGTTTAAGAT AAGGTTTACA
TTTCTTGTAT TAACGAATTT TATCTAGAAT TTTTATTCCC ACAAATTCTA TTCCAAATGT
5461 CTATTTTCAG CAGATATGTT AAAAAATAGA AGTGACTATA AAGACTTGAT AAAAATTATA
GATAAAAGTC GTCTATACAA TTTTTTATCT TCACTGATAT TTCTGAACTA TTTTTAATAT
5521 GTGACTGCAA ATGTTTTAGG AATATAATAA GATATAATAA CGGTGGTTGC TATTTTCTTT
CACTGACGTT TACAAAATCC TTATATTATT CTATATTATT GCCACCAACG ATAAAAGAAA
5581 AGCACAAGAC TAGTTAACAG GCTGTATTAA AAGATCTTTT CTTGAATTAA ATATTTTCAA
TCGTGTTCTG ATCAATTGTC CGACATAATT TTCTAGAAAA GAACTTAATT TATAAAAGTT
5641 TTTGATTAAA CCTACCTCAG CCATAAAGGC AAGCACATTT CATTTATACT ATGGGGATTT
AAACTAATTT GGATGGAGTC GGTATTTCCG TTCGTGTAAA GTAAATATGA TACCCCTAAA
5701 GAATAATTAT TACTGAAGAA GCTCTACCAA CAAAAAGTTT ATAGAGCTAT CATATTTAGT
CTTATTAATA ATGACTTCTT CGAGATGGTT GTTTTTCAAA TATCTCGATA GTATAAATCA
5761 CAAGAGATAA AGAGGGTTGT TAGGATATAT ATGCTATTTG AAAGGTATTT ATAAAAGAAG
GTTCTCTATT TCTCCCAACA ATCCTATATA TACGATAAAC TTTCCATAAA TATTTTCTTC
5821 AGTATATTTA TCAAAATTTC TCAGAACATC CAAATTTCAA GTTTATCATT TATCTTACAA
TCATATAAAT AGTTTTAAAG AGTCTTGTAG GTTTAAAGTT CAAATAGTAA ATAGAATGTT
5881 TATTTCAAAA ATATTAAAAT AGATACTGAA ATACAGAAGT AAATTAAAGA GAAAGTATTT
ATAAAGTTTT TATAATTTTA TCTATGACTT TATGTCTTCA TTTAATTTCT CTTTCATAAA
5941 TACTTGGTAA AAAAATTCTA GGTTGGACAG AGAGTGCCAG GAAACAAAAA CAATGAAAAA
ATGAACCATT TTTTTAAGAT CCAACCTGTC TCTCACGGTC CTTTGTTTTT GTTACTTTTT
6001 TGTGACCTGA CAGGAATTAT AGCTCAAAGT ATAGTAGTAA GTAATGAAAT GGCTTAAAAA
ACACTGGACT GTCCTTAATA TCGAGTTTCA TATCATCATT CATTACTTTA CCGAATTTTT
6061 TTGGTATATA AAATGCTAGT TATAAAATAA ACAAAATGCA ATAATATCCT CCCTACATGT
AACCATATAT TTTACGATCA ATATTTTATT TGTTTTACGT TATTATAGGA GGGATGTACA
6121 AATGAATTCT AGGTATTATG CTCTTTTTGG AAGTCTTGAC AATAAAAATT TTTTTAGAAG
TTACTTAAGA TCCATAATAC GAGAAAAACC TTCAGAACTG TTATTTTTAA AAAAATCTTC
6181 TTTATAGGCA TCTTGAATAA AGTGAAACAA ATTAAGAATT AGTATCCATG AGAAAAATAT
AAATATCCGT AGAACTTATT TCACTTTGTT TAATTCTTAA TCATAGGTAC TCTTTTTATA
6241 AGAACAATTT TCCTAATTTA GTTTGAAAAT CTGGGATTGA AGATGTGTGT CAAGAGATGT
TCTTGTTAAA AGGATTAAAT CAAACTTTTA GACCCTAACT TCTACACACA GTTCTCTACA
6301 TGGTGGCAAG AACATTTTTT TTTCAAGAAC TTATAAAAAT GCAACAAAAC AAACCATTTA
ACCACCGTTC TTGTAAAAAA AAAGTTCTTG AATATTTTTA CGTTGTTTTG TTTGGTAAAT
6361 ATACATTTTG GTCAAAATCA ATAATGTATT TTATTTTATG CTCCAAGGAG CATAAAATTG
TATGTAAAAC CAGTTTTAGT TATTACATAA AATAAAATAC GAGGTTCCTC GTATTTTAAC
6421 GGGACTGGGC AAGAGAAACT GACACCCTGG TAAATTACCA AGAGATAAGT ACACAGTTCT
CCCTGACCCG TTCTCTTTGA CTGTGGGACC ATTTAATGGT TCTCTATTCA TGTGTCAAGA
6481 ATGTAGAGAA AATAAGCATA GTGTATGATC TCTAAAATTA TGTGAGACAA AGGAGAGATG
TACATCTCTT TTATTCGTAT CACATACTAG AGATTTTAAT ACACTCTGTT TCCTCTCTAC
6541 ACATTAGGCA TGTGGGGATG AAGACTGAGT AGAGAAGAAA CAATCTAATC AGTCCAAGAA
TGTAATCCGT ACACCCCTAC TTCTGACTCA TCTCTTCTTT GTTAGATTAG TCAGGTTCTT
6601 AACATCTCGA TCAGTGGAAC AAATAGAAGA AATGCTAAAA TGAAACAGAA GTCTTACTGG
TTGTAGAGCT AGTCACCTTG TTTATCTTCT TTACGATTTT ACTTTGTCTT CAGAATGACC
6661 AAATAAAAGA TATGCATAAG ACAAAAATTC ATGAAAATCA CTTAGTTTAG CAGAGAAAAG
TTTATTTTCT ATACGTATTC TGTTTTTAAG TACTTTTAGT GAATCAAATC GTCTCTTTTC
6721 ATAAAAATAA AGTATGACCT TCTTCATATA CATTGTTTGA TCATATGCAC CTCAATAAAA
TATTTTTATT TCATACTGGA AGAAGTATAT GTAACAAACT AGTATACGTG GAGTTATTTT
6781 CTGAGTCTCC AACAGAAATG AAACATTAAT ATTTTGTTCA CTGCTCTAAT CCCAGAATCT
GACTCAGAGG TTGTCTTTAC TTTGTAATTA TAAAACAAGT GACGAGATTA GGGTCTTAGA
6841 AAGCGATATC TGGCAATAAA AATAATAAAT ATATATTTTT TAATAAATGA ATCAACCACT
TTCGCTATAG ACCGTTATTT TTATTATTTA TATATAAAAA ATTATTTACT TAGTTGGTGA
6901 TAATTTTTCT GTAAATATCT GTAACTTCTC TTCTGTCTTT CCAAAAACAC TCATAAGTAC
ATTAAAAAGA CATTTATAGA CATTGAAGAG AAGACAGAAA GGTTTTTGTG AGTATTCATG
6961 TGTGAATGAG ATGAAAAAGA GTGAAGTAGG ATATAGGCTG TTAGCAGAAA ACATCTGAAT
ACACTTACTC TACTTTTTCT CACTTCATCC TATATCCGAC AATCGTCTTT TGTAGACTTA
7021 GGCTGGCAGT GAAACATTAA CTTGAAATGT AAGATTAATG AGTAATAGTA AATTTTAACC
CCGACCGTCA CTTTGTAATT GAACTTTACA TTCTAATTAC TCATTATCAT TTAAAATTGG
7081 TTGGCCATAT GATAAAATGT TCATTAATAT TTTTCTAGAA TACAGGGCTT TTTGTTTTTG
AACCGGTATA CTATTTTACA AGTAATTATA AAAAGATCTT ATGTCCCGAA AAACAAAAAC
7141 CCATGAGGTT TGCAGGATCT TGGTTCCCTG ACCAGGGATC AAACCTGCAC TCCCCTGGAA
GGTACTCCAA ACGTCCTAGA ACCAAGGGAC TGGTCCCTAG TTTGGACGTG AGGGGACCTT
7201 GCATGGAGTC TTGGACATTT GTATTATACA CTATCTTTGG TTCCTTTTAA AGGGAAGTAA
CGTACCTCAG AACCTGTAAA CATAATATGT GATAGAAACC AAGGAAAATT TCCCTTCATT
7261 TTTTACTTAA ATAAGAAAAT AGATTGACAA GTAATACGCT GTTTCCTCAT CTTCCCATTC
AAAATGAATT TATTCTTTTA TCTAACTGTT CATTATGCGA CAAAGGAGTA GAAGGGTAAG
7321 ACAGGAATCG AGAGCCATGA AGGTCCTCAT CCTTGCCTGT CTGGTGGCTC TGGCCATTGC
TGTCCTTAGC TCTCGGTACT TCCAGGAGTA GGAACGGACA GACCACCGAG ACCGGTAACG
7381 GATCGCGGAT CCGAGCTCGG TACCAAGCTT AAGTTTAAAC CCGCTGATCA GCCTCGACTG
CTAGCGCCTA GGCTCGAGCC ATGGTTCGAA TTCAAATTTG GGCGACTAGT CGGAGCTGAC
7441 TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG
ACGGAAGATC AACGGTCGGT AGACAACAAA CGGGGAGGGG GCACGGAAGG AACTGGGACC
7501 AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA
TTCCACGGTG AGGGTGACAG GAAAGGATTA TTTTACTCCT TTAACGTAGC GTAACAGACT
7561 GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG
CATCCACAGT AAGATAAGAC CCCCCACCCC ACCCCGTCCT GTCGTTCCCC CTCCTAACCC
7621 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA
TTCTGTTATC GTCCGTACGA CCCCTACGCC ACCCGAGATA CCGAAGACTC CGCCTTTCTT
7681 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
GGTCGACCCC GAGATCCCCC ATAGGGGTGC GCGGGACATC GCCGCGTAAT TCGCGCCGCC
7741 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
CACACCACCA ATGCGCGTCG CACTGGCGAT GTGAACGGTC GCGGGATCGC GGGCGAGGAA
7801 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
AGCGAAAGAA GGGAAGGAAA GAGCGGTGCA AGCGGCCGAA AGGGGCAGTT CGAGATTTAG
7861 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
CCCCCGAGGG AAATCCCAAG GCTAAATCAC GAAATGCCGT GGAGCTGGGG TTTTTTGAAC
7921 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA
TAATCCCACT ACCAAGTGCA TCACCCGGTA GCGGGACTAT CTGCCAAAAA GCGGGAAACT
7981 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC
GCAACCTCAG GTGCAAGAAA TTATCACCTG AGAACAAGGT TTGACCTTGT TGTGAGTTGG
8041 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA
GATAGAGCCA GATAAGAAAA CTAAATATTC CCTAAAACGG CTAAAGCCGG ATAACCAATT
8101 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT
TTTTACTCGA CTAAATTGTT TTTAAATTGC GCTTAATTAA GACACCTTAC ACACAGTCAA
8161 AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA
TCCCACACCT TTCAGGGGTC CGAGGGGTCG TCCGTCTTCA TACGTTTCGT ACGTAGAGTT
8221 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG
AATCAGTCGT TGGTCCACAC CTTTCAGGGG TCCGAGGGGT CGTCCGTCTT CATACGTTTC
8281 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT
GTACGTAGAG TTAATCAGTC GTTGGTATCA GGGCGGGGAT TGAGGCGGGT AGGGCGGGGA
8341 AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC
TTGAGGCGGG TCAAGGCGGG TAAGAGGCGG GGTACCGACT GATTAAAAAA AATAAATACG
8401 AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG
TCTCCGGCTC CGGCGGAGAC GGAGACTCGA TAAGGTCTTC ATCACTCCTC CGAAAAAACC
8461 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA
TCCGGATCCG AAAACGTTTT TCGAGGGCCC TCGAACATAT AGGTAAAAGC CTAGACTAGT
8521 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC
TCTCTGTCCT ACTCCTAGCA AAGCGTACTA ACTTGTTCTA CCTAACGTGC GTCCAAGAGG
8581 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC
CCGGCGAACC CACCTCTCCG ATAAGCCGAT ACTGACCCGT GTTGTCTGTT AGCCGACGAG
8641 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA
ACTACGGCGG CACAAGGCCG ACAGTCGCGT CCCCGCGGGC CAAGAAAAAC AGTTCTGGCT
8701 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC
GGACAGGCCA CGGGACTTAC TTGACGTCCT GCTCCGTCGC GCCGATAGCA CCGACCGGTG
8761 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT
CTGCCCGCAA GGAACGCGTC GACACGAGCT GCAACAGTGA CTTCGCCCTT CCCTGACCGA
8821 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA
CGATAACCCG CTTCACGGCC CCGTCCTAGA GGACAGTAGA GTGGAACGAG GACGGCTCTT
8881 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC
TCATAGGTAG TACCGACTAC GTTACGCCGC CGACGTATGC GAACTAGGCC GATGGACGGG
8941 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT
TAAGCTGGTG GTTCGCTTTG TAGCGTAGCT CGCTCGTGCA TGAGCCTACC TTCGGCCAGA
9001 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC
ACAGCTAGTC CTACTAGACC TGCTTCTCGT AGTCCCCGAG CGCGGTCGGC TTGACAAGCG
9061 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG
GTCCGAGTTC CGCGCGTACG GGCTGCCGCT CCTAGAGCAG CACTGGGTAC CGCTACGGAC
9121 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT
GAACGGCTTA TAGTACCACC TTTTACCGGC GAAAAGACCT AAGTAGCTGA CACCGGCCGA
9181 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT
CCCACACCGC CTGGCGATAG TCCTGTATCG CAACCGATGG GCACTATAAC GACTTCTCGA
9241 TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA
ACCGCCGCTT ACCCGACTGG CGAAGGAGCA CGAAATGCCA TAGCGGCGAG GGCTAAGCGT
9301 GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA
CGCGTAGCGG AAGATAGCGG AAGAACTGCT CAAGAAGACT CGCCCTGAGA CCCCAAGCTT
9361 ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC
TACTGGCTGG TTCGCTGCGG GTTGGACGGT AGTGCTCTAA AGCTAAGGTG GCGGCGGAAG
9421 TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC
ATACTTTCCA ACCCGAAGCC TTAGCAAAAG GCCCTGCGGC CGACCTACTA GGAGGTCGCG
9481 GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT
CCCCTAGAGT ACGACCTCAA GAAGCGGGTG GGGTTGAACA AATAACGTCG AATATTACCA
9541 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT
ATGTTTATTT CGTTATCGTA GTGTTTAAAG TGTTTATTTC GTAAAAAAAG TGACGTAAGA
9601 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT
TCAACACCAA ACAGGTTTGA GTAGTTACAT AGAATAGTAC AGACATATGG CAGCTGGAGA
9661 AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
TCGATCTCGA ACCGCATTAG TACCAGTATC GACAAAGGAC ACACTTTAAC AATAGGCGAG
9721 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
TGTTAAGGTG TGTTGTATGC TCGGCCTTCG TATTTCACAT TTCGGACCCC ACGGATTACT
9781 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
CACTCGATTG AGTGTAATTA ACGCAACGCG AGTGACGGGC GAAAGGTCAG CCCTTTGGAC
9841 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
AGCACGGTCG ACGTAATTAC TTAGCCGGTT GCGCGCCCCT CTCCGCCAAA CGCATAACCC
9901 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
GCGAGAAGGC GAAGGAGCGA GTGACTGAGC GACGCGAGCC AGCAAGCCGA CGCCGCTCGC
9961 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA
CATAGTCGAG TGAGTTTCCG CCATTATGCC AATAGGTGTC TTAGTCCCCT ATTGCGTCCT
10021 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
TTCTTGTACA CTCGTTTTCC GGTCGTTTTC CGGTCCTTGG CATTTTTCCG GCGCAACGAC
10081 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
CGCAAAAAGG TATCCGAGGC GGGGGGACTG CTCGTAGTGT TTTTAGCTGC GAGTTCAGTC
10141 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
TCCACCGCTT TGGGCTGTCC TGATATTTCT ATGGTCCGCA AAGGGGGACC TTCGAGGGAG
10201 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
CACGCGAGAG GACAAGGCTG GGACGGCGAA TGGCCTATGG ACAGGCGGAA AGAGGGAAGC
10261 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
CCTTCGCACC GCGAAAGAGT ATCGAGTGCG ACATCCATAG AGTCAAGCCA CATCCAGCAA
10321 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
GCGAGGTTCG ACCCGACACA CGTGCTTGGG GGGCAAGTCG GGCTGGCGAC GCGGAATAGG
10381 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
CCATTGATAG CAGAACTCAG GTTGGGCCAT TCTGTGCTGA ATAGCGGTGA CCGTCGTCGG
10441 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
TGACCATTGT CCTAATCGTC TCGCTCCATA CATCCGCCAC GATGTCTCAA GAACTTCACC
10501 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
ACCGGATTGA TGCCGATGTG ATCTTCTTGT CATAAACCAT AGACGCGAGA CGACTTCGGT
10561 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
CAATGGAAGC CTTTTTCTCA ACCATCGAGA ACTAGGCCGT TTGTTTGGTG GCGACCATCG
10621 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
CCAAAAAAAC AAACGTTCGT CGTCTAATGC GCGTCTTTTT TTCCTAGAGT TCTTCTAGGA
10681 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
AACTAGAAAA GATGCCCCAG ACTGCGAGTC ACCTTGCTTT TGAGTGCAAT TCCCTAAAAC
10741 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
CAGTACTCTA ATAGTTTTTC CTAGAAGTGG ATCTAGGAAA ATTTAATTTT TACTTCAAAA
10801 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
TTTAGTTAGA TTTCATATAT ACTCATTTGA ACCAGACTGT CAATGGTTAC GAATTAGTCA
10861 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
CTCCGTGGAT AGAGTCGCTA GACAGATAAA GCAAGTAGGT ATCAACGGAC TGAGGGGCAG
10921 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
CACATCTATT GATGCTATGC CCTCCCGAAT GGTAGACCGG GGTCACGACG TTACTATGGC
10981 CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
GCTCTGGGTG CGAGTGGCCG AGGTCTAAAT AGTCGTTATT TGGTCGGTCG GCCTTCCCGG
11041 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
CTCGCGTCTT CACCAGGACG TTGAAATAGG CGGAGGTAGG TCAGATAATT AACAACGGCC
11101 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
CTTCGATCTC ATTCATCAAG CGGTCAATTA TCAAACGCGT TGCAACAACG GTAACGATGT
11161 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
CCGTAGCACC ACAGTGCGAG CAGCAAACCA TACCGAAGTA AGTCGAGGCC AAGGGTTGCT
11221 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
AGTTCCGCTC AATGTACTAG GGGGTACAAC ACGTTTTTTC GCCAATCGAG GAAGCCAGGA
11281 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
GGCTAGCAAC AGTCTTCATT CAACCGGCGT CACAATAGTG AGTACCAATA CCGTCGTGAC
11341 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
GTATTAAGAG AATGACAGTA CGGTAGGCAT TCTACGAAAA GACACTGACC ACTCATGAGT
11401 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
TGGTTCAGTA AGACTCTTAT CACATACGCC GCTGGCTCAA CGAGAACGGG CCGCAGTTAT
11461 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
GCCCTATTAT GGCGCGGTGT ATCGTCTTGA AATTTTCACG AGTAGTAACC TTTTGCAAGA
11521 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGATGTAACCCACT
AGCCCCGCTT TTGAGAGTTC CTAGAATGGC GACAACTCTA GGTCAAGCTA CATTGGGTGA
11581 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
GCACGTGGGT TGACTAGAAG TCGTAGAAAA TGAAAGTGGT CGCAAAGACC CACTCGTTTT
11641 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
TGTCCTTCCG TTTTACGGCG TTTTTTCCCT TATTCCCGCT GTGCCTTTAC AACTTATGAG
11701 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
TATGAGAAGG AAAAAGTTAT AATAACTTCG TAAATAGTCC CAATAACAGA GTACTCGCCT
11761 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
ATGTATAAAC TTACATAAAT CTTTTTATTT GTTTATCCCC AAGGCGCGTG TAAAGGGGCT
11821 AAAGTGCCAC CTGACGTC
TTTCACGGTG GACTGCAG

Claims (2)

1, a kind of promotor, its sequence is:
agatgatttt gcaaccccct gcctcaggag acactgggaa atttcctgag acatttttga 60
ttccaaaagc tgtgcagttg gtgcttctac catcttcgtg gtagaggtca aggatgctgc 120
taaacattct acaacacatt aagaaaaccc ccacaacaaa gaattcttcc gccaaaaata 180
tcaataatat gaaggttgaa aaatactggt ctagcatgta gtatgtgctc aatagcaagg 240
agagaaaaga aagccttcct cactgattaa tgcaaagaaa tagaggaaaa caatagaatg 300
ggaaagacta gagagctctt caagcaaatt agagatatca agggaacatt tcacgcaaag 360
atgggcacaa taaaggacag aaattttatg gaggagttgc tgatggagag ggaggcctgg 420
cgtgctgcga ttcctggggt cgcaaagagt cggacacaac tgagcgactg aattgaactg 480
aactgaactg gacaaagcag aagatattaa gaagaggtgg taagaataca cagaagaaca 540
atataaaaaa gatcttcatg acccagataa ccacgatgat gtgatcactc acctagagcc 600
agacaccctg gaatgcaaag tcaaacggcc ttagaaagcc tcactatgaa caaagctagt 660
ggaggtaatg gaattccagt tgagctattt caaatcttaa aaggtgatgc tgtgaaagtg 720
ctgcactcaa tatgtcagca aatttggaaa actcagcagt ggccacagga ctgccacaat 780
cccaaagaaa agcaatgaca aagaatgttc aaacacccac atgattgcac tcatctcaca 840
tgctagcaaa ataactctca aaattctcca agccaggctc caacagtacg tggaccatga 900
acttccagat gttcaagctg gatttagaaa aggcagagga accagagatc aaattgccaa 960
catccattgg atcatcaaaa aagcacgaga gttccagaaa aacatctgct ttattgacta 1020
cgctaaagcc tttgattgtg tggatcacaa taaactgtgg aaaattcttc aagagatggg 1080
aataccagac cactttacct gcctcctgag aaatctgtat acaggtccag aagcagcagt 1140
tagaactgga catggaacaa cagactggtt ccaaactgcg aaaggggtac atcaaggaat 1200
attcattgga aggattgatg ctgaagctga aactcctata ctttggccac ctaatgtgaa 1260
gatctgactc attggaaaag actccaatgc tgggaaagat tgaaggcagg agaagaggat 1320
gacagaggat gagatggttg gatgggatca ctgactcaat ggacatgagt ttgagtaagc 1380
tccaggggtt ggtggtggac aggaaagcct ggcgtgctgc agtccacaag gtcacaaaga 1440
ttcggacatg actgagtgac tgaactgata ctgatgtgct caacaaatgt atcttgaact 1500
tgtgtgaagt tctatggtca catgtaaagg aagaataatc aggattagct gtgtgtctta 1560
ggaatcaggg ttctgagttt tatgtgttca tagtatctgc tggttcacaa aacatttttc 1620
ttattctctg gttcttgatt tactttataa agtaatctta atagttatac ttcacataga 1680
tacgaaatta ttatatttgg ataatctcat ggaaaggatt aaatactcca tctattacga 1740
gtaatgctga actatctact cctacctaat aatttgtcag aattcactaa ttctgtgtta 1800
tattgtttct aaatctgaat cattatatga atcctcagta ttttgttttc cttcctctat 1860
attttggaat ttattaaaca gtgcttcaaa taatttttag gaaactgaag tttttagtaa 1920
cagctctatc tctaaatagc tttagtatct tgaaaaagta atacaaattc tcacatcctt 1980
aatttcctct tctctaaaat atctttaaaa tattctatga atgatatctc ttaatattta 2040
tttttttggc aatccaacac agcttatggg atcttagttc cccagtgagg gattatatcc 2100
atgccaactg cagtgaaagt acaaaatcct aaactggact caccagggat ttcccaatat 2160
ctcctctagt tcttatttct gaatattttt ggtcccttta ttgtactctt catccaactt 2220
ttctattgat ttctttcttg aggttattat ttacttggtt tcagttagaa atatatgcaa 2280
atctcaggac tgcatatttc agattcattg gccaatatgg gaaaaaacct ttggctgaac 2340
aaatcatgct tataaaaaat agtactagag catcctactt tgactatatc ttgctcctca 2400
ttcagggtta tctaatacaa tttccccaca tgaaattctt ttgcattata aaaatggaag 2460
ctcttaggta acattgcaaa aattcgagtt gctcatatgg cactttgctt cttactggtc 2520
attgtgttct gaggcttacc tggacaggtg gtacctgatg tcatcttaaa ttgctggctt 2580
tttgattttc cattggacaa gcttctttct ttagtatatt gttaaggatt tccttgatca 2640
agattttacc tacttttctg gtccaattgg tgagagacag tcataaggaa atgctgtgtt 2700
tattgcacaa tatgtaaagc atcttcctga gaaaataaaa gggaaatgtt gaatgggaag 2760
gatatgcttt cttttgtatt ccttttctga gaaatcaaac tttttcacct gtggccttgg 2820
ccaccaaaag ctaacaaata aaggcatatg aagtagccaa ggccttttct agttatatct 2880
ataacactga gttcatttca tcatttattt tcctgacttc ctcctgggtc catatgagca 2940
gtcttagaat gaatattagc tgaataatcc aaatacatag tagatgttga tttgggtttt 3000
ctaagcaatc caagacttgt atgacagtaa gatgtattac catccaacac acatctcagc 3060
atgatataaa tgcaaggtat attgtgaaga aaaattttta attatgtcaa agtgcttact 3120
ttagaaggtc atctatctgt cccaaagctg tgaatatata tattgaaggt aatgaataga 3180
tgaagctaac cttgtaaaaa tgagtagtgt gaaatacaac tacaattatg aacatctgtc 3240
actaaagagg caaagaaact tgaagattgc ttttgcaaat gggctcctat taataaaaag 3300
tacttttgag gtctggctca gactctattg tagtacttag ggtaagaccc tcctcctgta 3360
tgggctttca ttttctttct tgcttccctc atttgccctt ccatgaatac tagctgataa 3420
acattgacta taaaagatat gaggccaaac ttgagctgtc ccattttaat aaatctgtat 3480
aaataatatt tgttctacaa aagtattatc taaataaatg ttactttctg tcttaaaatc 3540
cctcaacaaa tccccactat ctagagaata agattgacat tccctggaat cacagcatgc 3600
tttgtctgcc attatctgac ccctttctct ttctctcttc tcacctccat ctactccttt 3660
ttccttgcaa ttcatgaccc agattcactg tttgatttgg cttgcatgtg tgtgtgctga 3720
gttgcgtctg actgttatca accccatgaa tgatagtcca ccaggctcta ctgtccatga 3780
aattttccag tcaagaatac tggagtggat tgcatttcct actccatttg attaatttag 3840
tgacttttaa atttcttttt ccatattcgg gagcctattc ttccttttta gtctatactc 3900
tcttcactct tcaggtctaa ggtatcatcg tgtgcttgtt agcttgttac tttctccatt 3960
atagcttaag cactaacaac tgttcaggtt ggcatgaaat tgtgttcttt gtgtggcctg 4020
tatatttctg ttgtgtatta gaatttaccc caagatctca aagacccact gaatactaaa 4080
gagacctcat tgtggttaca ataatttggg gactgggcca aaactaccgt gcatcccagc 4140
caagatctgt agctactgga caatttcatt tcctttatca gattgtgagt tattcctgtt 4200
aaaatgctcc ccagaatttc tggggacaga aaaataggaa gaattcattt cctaatcatg 4260
cagatttcta ggaattcaaa tccactgttg gttttatttc aaaccacaaa attagcatgc 4320
cattaaatac tatatataaa cagccactaa atcagatcat tatccattca gcttctcctt 4380
cacttcttct cctctacttt ggaaaaaagg taagaatctc agatataatt tcaggtgtat 4440
ctgctactca tctttatttt ggactaggtt aaaatgtaga aagaacataa ttgcttaaaa 4500
tagatcttaa aaataagggt gtttaagata aggtttacac tattttcagc agatatgtta 4560
aaaaatagaa gtgactataa agacttgata aaaattatag tgactgcaaa tgttttagga 4620
atataataag atataataac ggtggttgct attttcttta gcacaagact agttaacagg 4680
ctgtattaaa agatcttttc ttgaattaaa tattttcaat ttgattaaac ctacctcagc 4740
cataaaggca agcacatttc atttatacta tggggatttg aataattatt actgaagaag 4800
ctctaccaac aaaaagttta tagagctatc atatttagtc aagagataaa gagggttgtt 4860
aggatatata tgctatttga aaggtattta taaaagaaga gtatatttat caaaatttct 4920
cagaacatcc aaatttcaag tttatcattt atcttacaat atttcaaaaa tattaaaata 4980
gatactgaaa tacagaagta aattaaagag aaagtatttt acttggtaaa aaaattctag 5040
gttggacaga gagtgccagg aaacaaaaac aatgaaaaat gtgacctgac aggaattata 5100
gctcaaagta tagtagtaag taatgaaatg gcttaaaaat tggtatataa aatgctagtt 5160
ataaaataaa caaaatgcaa taatatcctc cctacatgta atgaattcta ggtattatgc 5220
tctttttgga agtcttgaca ataaaaattt ttttagaagt ttataggcat cttgaataaa 5280
gtgaaacaaa ttaagaatta gtatccatga gaaaaatata gaacaatttt cctaatttag 5340
tttgaaaatc tgggattgaa gatgtgtgtc aagagatgtt ggtggcaaga acattttttt 5400
ttcaagaact tataaaaatg caacaaaaca aaccatttaa tacattttgg tcaaaatcaa 5460
taatgtattt tattttatgc tccaaggagc ataaaattgg ggactgggca agagaaactg 5520
acaccctggt aaattaccaa gagataagta cacagttcta tgtagagaaa ataagcatag 5580
tgtatgatct ctaaaattat gtgagacaaa ggagagatga cattaggcat gtggggatga 5640
agactgagta gagaagaaac aatctaatca gtccaagaaa acatctcgat cagtggaaca 5700
aatagaagaa atgctaaaat gaaacagaag tcttactgga aataaaagat atgcataaga 5760
caaaaattca tgaaaatcac ttagtttagc agagaaaaga taaaaataaa gtatgacctt 5820
cttcatatac attgtttgat catatgcacc tcaataaaac tgagtctcca acagaaatga 5880
aacattaata ttttgttcac tgctctaatc ccagaatcta agcgatatct ggcaataaaa 5940
ataataaata tatatttttt aataaatgaa tcaaccactt aatttttctg taaatatctg 6000
taacttctct tctgtctttc caaaaacact cataagtact gtgaatgaga tgaaaaagag 6060
tgaagtagga tataggctgt tagcagaaaa catctgaatg gctggcagtg aaacattaac 6120
ttgaaatgta agattaatga gtaatagtaa attttaacct tggccatatg ataaaatgtt 6180
cattaatatt tttctagaat acagggcttt ttgtttttgc catgaggttt gcaggatctt 6240
ggttccctga ccagggatca aacctgcact cccctggaag catggagtct tggacatttg 6300
tattatacac tatctttggt tccttttaaa gggaagtaat tttacttaaa taagaaaata 6360
gattgacaag taatacgctg tttcctcatc ttcccattca caggaatcga gagccatgaa 6420
ggtcctcatc cttgcctgtc tggtggctct ggccattgcg atcgc。6465
2, a kind of expression vector that contains the described promotor of claim 1, its sequence is:
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60
ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120
cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180
ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240
gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300
tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360
cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420
attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480
atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540
atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600
tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660
actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720
aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780
gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840
ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900
gtttaaacgg gccctctaga cagatgattt tgcaaccccc tgcctcagga gacactggga 960
aatttcctga gacatttttg attccaaaag ctgtgcagtt ggtgcttcta ccatcttcgt 1020
ggtagaggtc aaggatgctg ctaaacattc tacaacacat taagaaaacc cccacaacaa 1080
agaattcttc cgccaaaaat atcaataata tgaaggttga aaaatactgg tctagcatgt 1140
agtatgtgct caatagcaag gagagaaaag aaagccttcc tcactgatta atgcaaagaa 1200
atagaggaaa acaatagaat gggaaagact agagagctct tcaagcaaat tagagatatc 1260
aagggaacat ttcacgcaaa gatgggcaca ataaaggaca gaaattttat ggaggagttg 1320
ctgatggaga gggaggcctg gcgtgctgcg attcctgggg tcgcaaagag tcggacacaa 1380
ctgagcgact gaattgaact gaactgaact ggacaaagca gaagatatta agaagaggtg 1440
gtaagaatac acagaagaac aatataaaaa agatcttcat gacccagata accacgatga 1500
tgtgatcact cacctagagc cagacaccct ggaatgcaaa gtcaaacggc cttagaaagc 1560
ctcactatga acaaagctag tggaggtaat ggaattccag ttgagctatt tcaaatctta 1620
aaaggtgatg ctgtgaaagt gctgcactca atatgtcagc aaatttggaa aactcagcag 1680
tggccacagg actgccacaa tcccaaagaa aagcaatgac aaagaatgtt caaacaccca 1740
catgattgca ctcatctcac atgctagcaa aataactctc aaaattctcc aagccaggct 1800
ccaacagtac gtggaccatg aacttccaga tgttcaagct ggatttagaa aaggcagagg 1860
aaccagagat caaattgcca acatccattg gatcatcaaa aaagcacgag agttccagaa 1920
aaacatctgc tttattgact acgctaaagc ctttgattgt gtggatcaca ataaactgtg 1980
gaaaattctt caagagatgg gaataccaga ccactttacc tgcctcctga gaaatctgta 2040
tacaggtcca gaagcagcag ttagaactgg acatggaaca acagactggt tccaaactgc 2100
gaaaggggta catcaaggaa tattcattgg aaggattgat gctgaagctg aaactcctat 2160
actttggcca cctaatgtga agatctgact cattggaaaa gactccaatg ctgggaaaga 2220
ttgaaggcag gagaagagga tgacagagga tgagatggtt ggatgggatc actgactcaa 2280
tggacatgag tttgagtaag ctccaggggt tggtggtgga caggaaagcc tggcgtgctg 2340
cagtccacaa ggtcacaaag attcggacat gactgagtga ctgaactgat actgatgtgc 2400
tcaacaaatg tatcttgaac ttgtgtgaag ttctatggtc acatgtaaag gaagaataat 2460
caggattagc tgtgtgtctt aggaatcagg gttctgagtt ttatgtgttc atagtatctg 2520
ctggttcaca aaacattttt cttattctct ggttcttgat ttactttata aagtaatctt 2580
aatagttata cttcacatag atacgaaatt attatatttg gataatctca tggaaaggat 2640
taaatactcc atctattacg agtaatgctg aactatctac tcctacctaa taatttgtca 2700
gaattcacta attctgtgtt atattgtttc taaatctgaa tcattatatg aatcctcagt 2760
attttgtttt ccttcctcta tattttggaa tttattaaac agtgcttcaa ataattttta 2820
ggaaactgaa gtttttagta acagctctat ctctaaatag ctttagtatc ttgaaaaagt 2880
aatacaaatt ctcacatcct taatttcctc ttctctaaaa tatctttaaa atattctatg 2940
aatgatatct cttaatattt atttttttgg caatccaaca cagcttatgg gatcttagtt 3000
ccccagtgag ggattatatc catgccaact gcagtgaaag tacaaaatcc taaactggac 3060
tcaccaggga tttcccaata tctcctctag ttcttatttc tgaatatttt tggtcccttt 3120
attgtactct tcatccaact tttctattga tttctttctt gaggttatta tttacttggt 3180
ttcagttaga aatatatgca aatctcagga ctgcatattt cagattcatt ggccaatatg 3240
ggaaaaaacc tttggctgaa caaatcatgc ttataaaaaa tagtactaga gcatcctact 3300
ttgactatat cttgctcctc attcagggtt atctaataca atttccccac atgaaattct 3360
tttgcattat aaaaatggaa gctcttaggt aacattgcaa aaattcgagt tgctcatatg 3420
gcactttgct tcttactggt cattgtgttc tgaggcttac ctggacaggt ggtacctgat 3480
gtcatcttaa attgctggct ttttgatttt ccattggaca agcttctttc tttagtatat 3540
tgttaaggat ttccttgatc aagattttac ctacttttct ggtccaattg gtgagagaca 3600
gtcataagga aatgctgtgt ttattgcaca atatgtaaag catcttcctg agaaaataaa 3660
agggaaatgt tgaatgggaa ggatatgctt tcttttgtat tccttttctg agaaatcaaa 3720
ctttttcacc tgtggccttg gccaccaaaa gctaacaaat aaaggcatat gaagtagcca 3780
aggccttttc tagttatatc tataacactg agttcatttc atcatttatt ttcctgactt 3840
cctcctgggt ccatatgagc agtcttagaa tgaatattag ctgaataatc caaatacata 3900
gtagatgttg atttgggttt tctaagcaat ccaagacttg tatgacagta agatgtatta 3960
ccatccaaca cacatctcag catgatataa atgcaaggta tattgtgaag aaaaattttt 4020
aattatgtca aagtgcttac tttagaaggt catctatctg tcccaaagct gtgaatatat 4080
atattgaagg taatgaatag atgaagctaa ccttgtaaaa atgagtagtg tgaaatacaa 4140
ctacaattat gaacatctgt cactaaagag gcaaagaaac ttgaagattg cttttgcaaa 4200
tgggctccta ttaataaaaa gtacttttga ggtctggctc agactctatt gtagtactta 4260
gggtaagacc ctcctcctgt atgggctttc attttctttc ttgcttccct catttgccct 4320
tccatgaata ctagctgata aacattgact ataaaagata tgaggccaaa cttgagctgt 4380
cccattttaa taaatctgta taaataatat ttgttctaca aaagtattat ctaaataaat 4440
gttactttct gtcttaaaat ccctcaacaa atccccacta tctagagaat aagattgaca 4500
ttccctggaa tcacagcatg ctttgtctgc cattatctga cccctttctc tttctctctt 4560
ctcacctcca tctactcctt tttccttgca attcatgacc cagattcact gtttgatttg 4620
gcttgcatgt gtgtgtgctg agttgcgtct gactgttatc aaccccatga atgatagtcc 4680
accaggctct actgtccatg aaattttcca gtcaagaata ctggagtgga ttgcatttcc 4740
tactccattt gattaattta gtgactttta aatttctttt tccatattcg ggagcctatt 4800
cttccttttt agtctatact ctcttcactc ttcaggtcta aggtatcatc gtgtgcttgt 4860
tagcttgtta ctttctccat tatagcttaa gcactaacaa ctgttcaggt tggcatgaaa 4920
ttgtgttctt tgtgtggcct gtatatttct gttgtgtatt agaatttacc ccaagatctc 4980
aaagacccac tgaatactaa agagacctca ttgtggttac aataatttgg ggactgggcc 5040
aaaactaccg tgcatcccag ccaagatctg tagctactgg acaatttcat ttccrrtatc 5100
agattgtgag ttattcctgt taaaatgctc cccagaattt ctggggacag aaaaatagga 5160
agaattcatt tcctaatcat gcagatttct aggaattcaa atccactgtt ggttttattt 5220
caaaccacaa aattagcatg ccattaaata ctatatataa acagccacta aatcagatca 5280
ttatccattc agcttctcct tcacttcttc tcctctactt tggaaaaaag gtaagaatct 5340
cagatataat ttcaggtgta tctgctactc atctttattt tggactaggt taaaatgtag 5400
aaagaacata attgcttaaa atagatctta aaaataaggg tgtttaagat aaggtttaca 5460
ctattttcag cagatatgtt aaaaaataga agtgactata aagacttgat aaaaattata 5520
gtgactgcaa atgttttagg aatataataa gatataataa cggtggttgc tattttcttt 5580
agcacaagac tagttaacag gctgtattaa aagatctttt cttgaattaa atattttcaa 5640
tttgattaaa cctacctcag ccataaaggc aagcacattt catttatact atggggattt 5700
gaataattat tactgaagaa gctctaccaa caaaaagttt atagagctat catatttagt 5760
caagagataa agagggttgt taggatatat atgctatttg aaaggtattt ataaaagaag 5820
agtatattta tcaaaatttc tcagaacatc caaatttcaa gtttatcatt tatcttacaa 5880
tatttcaaaa atattaaaat agatactgaa atacagaagt aaattaaaga gaaagtattt 5940
tacttggtaa aaaaattcta ggttggacag agagtgccag gaaacaaaaa caatgaaaaa 6000
tgtgacctga caggaattat agctcaaagt atagtagtaa gtaatgaaat ggcttaaaaa 6060
ttggtatata aaatgctagt tataaaataa acaaaatgca ataatatcct ccctacatgt 6120
aatgaattct aggtattatg ctctttttgg aagtcttgac aataaaaatt tttttagaag 6180
tttataggca tcttgaataa agtgaaacaa attaagaatt agtatccatg agaaaaatat 6240
agaacaattt tcctaattta gtttgaaaat ctgggattga agatgtgtgt caagagatgt 6300
tggtggcaag aacatttttt tttcaagaac ttataaaaat gcaacaaaac aaaccattta 6360
atacattttg gtcaaaatca ataatgtatt ttattttatg ctccaaggag cataaaattg 6420
gggactgggc aagagaaact gacaccctgg taaattacca agagataagt acacagttct 6480
atgtagagaa aataagcata gtgtatgatc tctaaaatta tgtgagacaa aggagagatg 6540
acattaggca tgtggggatg aagactgagt agagaagaaa caatctaatc agtccaagaa 6600
aacatctcga tcagtggaac aaatagaaga aatgctaaaa tgaaacagaa gtcttactgg 6660
aaataaaaga tatgcataag acaaaaattc atgaaaatca cttagtttag cagagaaaag 6720
ataaaaataa agtatgacct tcttcatata cattgtttga tcatatgcac ctcaataaaa 6780
ctgagtctcc aacagaaatg aaacattaat attttgttca ctgctctaat cccagaatct 6840
aagcgatatc tggcaataaa aataataaat atatattttt taataaatga atcaaccact 6900
taatttttct gtaaatatct gtaacttctc ttctgtcttt ccaaaaacac tcataagtac 6960
tgtgaatgag atgaaaaaga gtgaagtagg atataggctg ttagcagaaa acatctgaat 7020
ggctggcagt gaaacattaa cttgaaatgt aagattaatg agtaatagta aattttaacc 7080
ttggccatat gataaaatgt tcattaatat ttttctagaa tacagggctt tttgtttttg 7140
ccatgaggtt tgcaggatct tggttccctg accagggatc aaacctgcac tcccctggaa 7200
gcatggagtc ttggacattt gtattataca ctatctttgg ttccttttaa agggaagtaa 7260
ttttacttaa ataagaaaat agattgacaa gtaatacgct gtttcctcat cttcccattc 7320
acaggaatcg agagccatga aggtcctcat ccttgcctgt ctggtggctc tggccattgc 7380
gatcgcggat ccgagctcgg taccaagctt aagtttaaac ccgctgatca gcctcgactg 7440
tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 7500
aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 7560
gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 7620
aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 7680
ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg 7740
gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 7800
tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 7860
gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 7920
attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 7980
cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 8040
ctatctcggt ctattctttt gatttatrag ggattttgcc gatttcggcc tattggttaa 8100
aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt 8160
agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 8220
ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 8280
catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 8340
aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 8400
agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg 8460
aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca 8520
agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 8580
ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 8640
tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 8700
cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac 8760
gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 8820
gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 8880
agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 8940
attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 9000
tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 9060
caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 9120
cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 9180
gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 9240
tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 9300
gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 9360
atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 9420
tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 9480
ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 9540
tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 9600
agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 9660
agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 9720
acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 9780
gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 9840
tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 9900
cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 9960
gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 10020
aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 10080
gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 10140
aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 10200
gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 10260
ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 10320
cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 10380
ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 10440
actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 10500
tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 10560
gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 10620
ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 10680
ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 10740
gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 10800
aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 10860
gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 10920
gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 10980
cgagacccac gctcaccggc tccagattta tccagattta tcagcaataa cggaagggcc 11040
gagcgcagaa gtggtcctgc aactttatcc aactttatcc gcctccatcc ttgttgccgg 11100
gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 11160
ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 11220
tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 11280
ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 11340
cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 11400
accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 11460
cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 11520
tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 11580
cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 11640
acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 11700
atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 11760
tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 11820
aaagtgccac ctgacgtc。11838
CNB2004100731643A 2004-10-11 2004-10-11 Expression vector for promotor of casein gene of milch goats of 'guanzhong' Expired - Fee Related CN100503832C (en)

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CN103911393B (en) * 2012-12-31 2019-01-18 上海滔滔转基因工程股份有限公司 A kind of building and application of human transferrin mammary gland specific expression vector
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CN105779496A (en) * 2016-03-18 2016-07-20 青岛农业大学 Method of utilizing goat mammary gland bioreactor to prepare Canine distemper fusion protein gene recombinant vaccine

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