CN100493568C - Rhizome Anemarrhenae total saponin and its preparation method - Google Patents
Rhizome Anemarrhenae total saponin and its preparation method Download PDFInfo
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- CN100493568C CN100493568C CNB2005100546331A CN200510054633A CN100493568C CN 100493568 C CN100493568 C CN 100493568C CN B2005100546331 A CNB2005100546331 A CN B2005100546331A CN 200510054633 A CN200510054633 A CN 200510054633A CN 100493568 C CN100493568 C CN 100493568C
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Abstract
The invention provides Rhizome Anemarrhenae use of medicine preparation for treating or preventing diabetes mellitus. Specifically, the Rhizome Anemarrhenae inhibits Alpha-glucosidase, reduces the blood sugar after dinner, promotes the glucose metabolism, balances the blood sugar, reduces the blood fat, advances the insulin secretion and keeps the blood sugar stability.
Description
Technical field
The present invention relates to the new medical usage of Rhizoma Anemarrhenae total saponins, relate to the application of Rhizoma Anemarrhenae total saponins in the medicine of preparation treatment or prevent diabetes particularly.
Background technology
The Rhizoma Anemarrhenae is the dry rhizome of hundred standing grain section (liliaceae) the plant Rhizoma Anemarrhenaes (Anemarrhena asphodeloides Bge.).Studies have shown that in a large number Rhizoma Anemarrhenae total saponins is its main active, 17 kinds of monomer saponins appear in the newspapers, but the relevant Rhizoma Anemarrhenae total saponins of Shang Weijian suppresses alpha-glucosidase activity, reduces post-prandial glycemia; Promote carbohydrate metabolism, balance blood sugar, blood fat reducing promotes insulin secretion, keeps the report of the medicine of blood glucose long-term stability.
Summary of the invention
The invention provides the application of Rhizoma Anemarrhenae total saponins in the medicine of preparation treatment or prevent diabetes.
The invention provides Rhizoma Anemarrhenae total saponins suppressing alpha-glucosidase, reduce post-prandial glycemia; Promote carbohydrate metabolism, balance blood sugar, blood fat reducing promotes insulin secretion, keeps the application of blood glucose aspect steady in a long-term.
Rhizoma Anemarrhenae total saponins of the present invention, 100%〉Rhizoma Anemarrhenae total saponins content 〉=50%, 100% wherein〉content 〉=30% of greenbrier Chinese yam sapogenin, it can prepare by the following method: rhizoma ane marrhenae is ground into coarse powder, add soak with ethanol, room temperature supersound extraction then, extracting solution merge filters, and filtrate concentrates, macroporous adsorbent resin on the concentrated solution, different alcoholic solution eluting, eluent is evaporated to stiff paste, the dry Rhizoma Anemarrhenae total saponins that gets; But be not limited thereto.
The specific embodiment
Preparation example 1: preparation Rhizoma Anemarrhenae total saponins
Rhizoma ane marrhenae is ground into coarse powder, adding 70% ethanol soaked 4 hours earlier, the room temperature supersound extraction is three times then, the solvent multiple is 4:4:3, each 3 hours of extraction time, extracting solution merges filtration, filtrate decompression is concentrated into ethanol content about 30%, the AB-8 macroporous adsorbent resin of handling well on the concentrated solution, AB-8 macroporous adsorbent resin behind the last sample is used 2 column volumes of 30% ethanol elution respectively, 1 column volume of 50% ethanol elution, 2 column volumes of 95% ethanol elution, 95% ethanol elution is evaporated to stiff paste, and the stiff paste vacuum drying is got Rhizoma Anemarrhenae total saponins; Its total saponin content 54%, the content 32.3% of greenbrier Chinese yam sapogenin.
Preparation example 2: preparation Rhizoma Anemarrhenae total saponins
Rhizoma ane marrhenae is ground into coarse powder, adding 70% ethanol soaked 4 hours earlier, the room temperature supersound extraction is three times then, the solvent multiple is 4:4:3, each 3 hours of extraction time, extracting solution merges filtration, filtrate decompression is concentrated into ethanol content about 30%, the AB-8 macroporous adsorbent resin of handling well on the concentrated solution, AB-8 macroporous adsorbent resin behind the last sample is used 2 column volumes of 30% ethanol elution respectively, 1 column volume of 50% ethanol elution, 2 column volumes of 95% ethanol elution, 95% ethanol elution is evaporated to stiff paste, and the stiff paste vacuum drying is got Rhizoma Anemarrhenae total saponins; Its total saponin content 84%, the content 52.3% of greenbrier Chinese yam sapogenin.
Test example one: to the body outer suppressioning experiment of alpha-glucosidase
1. material:
Rhizoma Anemarrhenae total saponins: by preparation example 1 preparation;
Rat: Shandong Province's natural drug Engineering Technical Research Centre provides
Glucobay (acarbose): Bayer medicines and health protection company limited, lot number: 101181
2. method:
Get rat upper small intestine 20g, add the PH6.8 phosphate buffer 60ml of 4 ℃ of pre-coolings, homogenate, 4 ℃, the centrifugal 20min of 4000rpm get the supernatant branch and pack in the test tube, and-20 ℃ of freezing preservations are standby; Get enzyme liquid (small intestinal homogenate) 0.2ml and add PH6.8 phosphate buffer 0.7ml, 37 ℃ of water-bath 10min add 0.5mol/L sucrose 0.1ml, behind the reaction 20min, add 1ml0.1mol/LNaCO
3Cessation reaction; Enzymatic Determination of Glucose concentration is divided 3 groups, blank group, Rhizoma Anemarrhenae total saponins group and glucobay (acarbose) group, every group of tee pipe, parallel assay; Will be at 37 ℃, generate the 0.01mmol glucose in the per minute 1L solution and be defined as an enzyme activity unit, see Table 1.
Table 1 Rhizoma Anemarrhenae total saponins is to the vitro inhibition of alpha-glucosidase activity
Test example two: to the influence experiment of normal mouse blood sugar
1. material:
White mice: Shandong Province's natural drug Engineering Technical Research Centre provides;
Rhizoma Anemarrhenae total saponins: by the preparation of preparation example 2 methods;
2. method:
Get 50 of white mice, male and female half and half, body weight 24-28g surveys fasting glucose, is divided into 5 groups at random: normal control group, 4 dosage groups of Rhizoma Anemarrhenae total saponins (50mg/Kg, 150mg/Kg, 450mg/Kg, 1350mg/Kg).Continuous irrigation stomach 7 days, blood sugar concentration is surveyed in the docking blood sampling behind the last administration 7h; The sacrificed by decapitation mice is got the centrifugal blood separation of serum then, puts-20 ℃ of refrigerators and preserves, and it is standby to do insulin radioimmunoassay; Mice is cut open the belly, and portal vein is pressed 10ml/ dosage only in liver, with syringe normal saline is slowly injected liver, remained blood in the liver is rinsed out, take out liver, weigh, add PH7.4 isotonic phosphate buffer liquid 50ml by the 1g liver and prepare homogenate with tissue grinder, get homogenate 0.5ml, add 4% sulfosalicylic acid solution 0.5ml, fully mixing, centrifugal, abandon precipitation, anthrone method is measured hepatic glycogen content, sees Table 2.
Table 2 Rhizoma Anemarrhenae total saponins is to the influence of normal mouse blood sugar, blood insulin levels and hepatic glycogen content
Compare with the normal control group: * P＜0.05, * * P＜0.01
Test example three: to the influence experiment of diabetic mice blood glucose
1. material:
White mice: Shandong Province's natural drug Engineering Technical Research Centre provides;
Rhizoma Anemarrhenae total saponins: by the preparation of preparation example method;
Glyburide: Tianjin Lik-Sang pharmaceutical factory product, lot number 990304
2. method
Get 150 of white mice, male and female half and half, body weight 24-28g, 10 is the normal control group, all the other tail vein injection alloxan 55mg/Kg, survey fasting glucose behind the 72h, choose blood glucose value 60 of the hyperglycemia mices of 11mmo/L, be divided into 6 groups at random by the blood glucose height: diabetes model matched group, glyburide group and 4 dosage groups of Rhizoma Anemarrhenae total saponins (50mg/Kg, 150mg/Kg, 450mg/Kg 1350mg/Kg), makes the blood glucose there was no significant difference of each group.Gastric infusion 10d, 7h after the last administration, blood sugar concentration is surveyed in the docking blood sampling; The sacrificed by decapitation mice is got the centrifugal blood separation of serum then, puts-20 ℃ of refrigerators and preserves, and it is standby to do insulin radioimmunoassay; Mice is cut open the belly, and portal vein is pressed 10ml/ dosage only in liver, with syringe normal saline is slowly injected liver, remained blood in the liver is rinsed out, take out liver, weigh, add PH7.4 isotonic phosphate buffer liquid 50ml by the 1g liver and prepare homogenate with tissue grinder, get homogenate 0.5ml, add 4% sulfosalicylic acid solution 0.5ml, fully mixing, centrifugal, abandon precipitation, anthrone method is measured hepatic glycogen content, sees Table 3.
Table 3 Rhizoma Anemarrhenae total saponins is to the influence of alloxan induced hyperglycemia mice blood glucose, blood insulin levels and hepatic glycogen content
Compare with the normal control group:
△P＜0.05,
△ △P＜0.01; Compare with model control group: * P＜0.05, * * P＜0.01 test example four: the influence experiment of normal mouse carbohydrate tolerance
1. material:
Rhizoma Anemarrhenae total saponins: by preparation example 1 preparation;
Rat: Shandong Province's natural drug Engineering Technical Research Centre provides
Glucobay (acarbose): Bayer medicines and health protection company limited, lot number: 101181
2. method:
Get 60 of white mice, male and female half and half, body weight 24-28g surveys fasting glucose, be divided into 6 groups at random: normal control group, glucobay (acarbose) group and 4 dosage groups of Rhizoma Anemarrhenae total saponins (50mg/Kg, 150mg/Kg, 450mg/Kg, 1350mg/Kg), the blood glucose there was no significant difference that each is organized.After the administration, irritate stomach,, see Table 4 respectively at surveying blood glucose to 0.5h, 1h, 2h behind the starch with 10g/Kg dosage starch.
Table 4 Rhizoma Anemarrhenae total saponins is to the influence of normal mouse carbohydrate tolerance
Compare with the normal control group: * P＜0.05, * * P＜0.01
Test example five: to the influence experiment of diabetic mice carbohydrate tolerance
1. material:
Rhizoma Anemarrhenae total saponins: by preparation example 1 preparation;
Rat: Shandong Province's natural drug Engineering Technical Research Centre provides
Glucobay (acarbose): Bayer medicines and health protection company limited, lot number: 101181
2. method:
Get 150 of white mice, male and female half and half, body weight 24-28g, 10 as the normal control group, all the other tail vein injection alloxan 55mg/Kg survey fasting glucose behind the 72h, choose blood glucose value〉50 of the hyperglycemia mices of 11mmo/L, be divided into 5 groups at random: 4 dosage groups of glucobay (acarbose) group and Rhizoma Anemarrhenae total saponins (50mg/Kg, 150mg/Kg, 450mg/Kg, 1350mg/Kg), the blood glucose there was no significant difference that each is organized.After the administration, irritate stomach,, see Table 5 respectively at surveying blood glucose to 0.5h, 1h, 2h behind the starch with 10g/Kg dosage starch.
Table 5 Rhizoma Anemarrhenae total saponins is to the influence of alloxan model mice carbohydrate tolerance
Compare with model control group: * P＜0.05, * * P＜0.01
Claims (2)
1. a Rhizoma Anemarrhenae total saponins is prepared by following method: rhizoma ane marrhenae is pulverized, added 70% soak with ethanol 4 hours, room temperature supersound extraction three times, solvent multiple are 4,4,3, extraction time is 3 hours, merging filtrate, being evaporated to pure content after the filtration is 30%, on AB-8 macroporous resin handled well, with 2 column volumes of 30% ethanol elution, 1 column volume of 50% ethanol elution, 2 column volumes of 95% ethanol elution are collected 95% ethanol elution, be concentrated into cream, vacuum drying promptly.
2. the preparation method of a Rhizoma Anemarrhenae total saponins is pulverized rhizoma ane marrhenae, adds 70% soak with ethanol 4 hours, room temperature supersound extraction three times, solvent multiple are 4,4,3, extraction time is 3 hours, merging filtrate, being evaporated to pure content after the filtration is 30%, on AB-8 macroporous resin handled well, with 2 column volumes of 30% ethanol elution, 1 column volume of 50% ethanol elution, 2 column volumes of 95% ethanol elution are collected 95% ethanol elution, be concentrated into cream, vacuum drying promptly.
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| CNB2005100546331A CN100493568C (en) | 2004-03-08 | 2005-03-07 | Rhizome Anemarrhenae total saponin and its preparation method |
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| CNB2005100546331A CN100493568C (en) | 2004-03-08 | 2005-03-07 | Rhizome Anemarrhenae total saponin and its preparation method |
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| CN100493568C true CN100493568C (en) | 2009-06-03 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008001673A (en) * | 2006-06-26 | 2008-01-10 | Kano Shiyoujiyuan:Kk | Sugar absorption inhibiting or stomach mucous membrane-protecting composition containing dioscin as active ingredient, and dioscin analog compound |
| CN104606459B (en) * | 2014-12-29 | 2017-11-14 | 辽宁中医药大学 | A kind of preparation method of the hypoglycemic extract of the wind-weed |
| CN105395808B (en) * | 2015-12-31 | 2019-09-13 | 南京中医药大学 | It is a kind of for treating the synergism medicine composition of diabetes |
| CN105596684A (en) * | 2016-02-01 | 2016-05-25 | 北京宜生堂医药科技研究有限公司 | Rhizoma anemarrhenae extract and lily and rhizoma anemarrhenae extract |
| CN111732624B (en) * | 2020-07-03 | 2022-09-20 | 河南大学 | Steroid saponin compound, preparation method thereof and application thereof in preparing medicament for treating type 2 diabetes |
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2005
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Non-Patent Citations (6)
| Title |
|---|
| Isolation of pseudoprototimo saponin AIII fromrhizomesofAnemarrhena asphodeloides and its hypoglycemicactivityinstreptozotocin induced diabetic mice.. Nkashima N,Kimura I,Kimura M.Journal of Natural Products,Vol.56 No.3. 1993 |
| Isolation of pseudoprototimo saponin AIII fromrhizomesofAnemarrhena asphodeloides and its hypoglycemicactivityinstreptozotocin induced diabetic mice.. Nkashima N,Kimura I,Kimura M.Journal of Natural Products,Vol.56 No.3. 1993 * |
| 知母的化学成分及药理作用研究进展. 杨丽蓉.国外医学中医中药分册，第24卷第4期. 2002 |
| 知母的化学成分及药理作用研究进展. 杨丽蓉.国外医学中医中药分册，第24卷第4期. 2002 * |
| 降糖中药对α-葡萄糖苷酶抑制作用的研究. 沈忠明等.中国生化药物杂志，第21卷第2期. 2002 |
| 降糖中药对α-葡萄糖苷酶抑制作用的研究. 沈忠明等.中国生化药物杂志，第21卷第2期. 2002 * |
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